scholarly journals The morphological composition of the blood and the peculiarities of metabolism in gonadectomized cockerels of the Adler silvery breed

Author(s):  
I. Cheverda ◽  
M. Zakharenko

The article reflects the results of a study of the morphological composition of blood, indicators of metabolism and the content of hormones in the blood plasma of gonadectomized cockerels of the Adler silvery breed. In the experiment, 40 males were used at the age of 6 weeks, of which two groups were formed, a control and an experimental, 20 heads each. It was found that during the preparatory (leveling) period, the clinical state, morphological composition of blood and metabolic parameters in the experimental group of males did not differ from each other and were within the limits of their physiological values. There were also no differences in blood glucose concentration, protein content, triglycerol, cholesterol, uric acid, as well as the activity of alkaline phosphatase, alanine and aspartate aminotransferase in the blood plasma of cockerels in the experimental and control groups during the preparatory period. Gonadectomy of males by surgical intervention at the age of six weeks did not significantly affect the morphology of their blood on the third day, with the exception of ESR, the value of which in birds of the experimental group was 1.9 times higher than in the control. In gonadectomized males, the number of platelets in the blood decreased by 30.7% compared to the control, which indicates a decrease in platelet activity and is probably associated with their participation in blood coagulation processes in the body after gonadectomy. Other indicators of the morphological composition of blood, namely, the number of erythrocytes, leukocytes, lymphocytes, eosinophils and neutrophils in gonadectomized males did not change compared with the control. It was found that the concentration of glucose in the blood, as well as total protein and uric acid in the blood plasma of males who underwent gonadectomy on the third day of the research period were at the level of the values of the control group birds and corresponded to the physiological values of these parameters in clinically healthy birds. On the 125th day of the main period in gonadectomized males, the level of glucose and protein, as well as indicators of lipid metabolism, namely, the concentration of triglycerol and cholesterol in the blood plasma did not change, while the content of uric acid increased by 23.7% compared with the control. It was found that the content of cortisol in the blood plasma of gonadectomized males was 3.2 times lower, and testosterone 10.2 times lower than in the control. So, gonadectomy of cockerels does not affect the morphological composition of the blood, indicators of the metabolic status of the bird, but it helps to reduce the content of cortisol and testosterone in the blood plasma. Key words: roosters, gonadectomy, blood morphology, metabolic parameters, enzyme activity, cortisol, testosterone.

2020 ◽  
Vol 5 (2) ◽  
pp. 63-71
Author(s):  
Vladimir Nikulin ◽  
Aleksandra Mustafina

The aim of the study is to increase the productive qualities of broiler chickens by including ultrafine silicon oxide into main diet. During the experiment, the biological effect of ultrafine silicon oxide on broiler chickens was estab-lished. Use of ultrafine SiO2 particles for poultry feeding contributed to an increase in the number of red blood cells and content of total protein and albumins. By the end of the experiment, the number of red blood cells in birds in-creased by 17.43% (P≤0.001) – in the blood of birds of the first experimental group, 16.51% (P≤0.01) – the second one, 20.80% (P≤0.001) – the third experimental and 21.71% (P≤0.001) – the fourth experimental group, compared with the indicator of the control group. The amount of total protein in blood serum of chickens of the first and the second experimental groups increased by 1.36-1.39 %, in the third and fourth ones there was a significant (P≤0.05) increase by 5.45 and 3.05%, respectively. The blood glucose content of chickens in the experimental groups is higher by 8.04-23.65% compared to this indicator with ones in the control group. During the experiment feed con-sumption per 1 kg gain of live weight decreased: in the first experimental group by 3.00 % in the second by – 0.50 %, the third – 6.00 % the fourth– by 4.50 %, compared to this with the control group. The chicken’s vibrancy of the first the experimental group was higher by 4.77 %, the second – by 6.20 %, the third – by 19.25 % and the fourth– by 11.59% than in the control one. Consequently, when converting the feed energy into the body energy of a broiler chicken, the energy conversion coefficient of the experimental group of poultry is higher than that of the control one by 7.16-21.76 %. Thus, the most optimal dose for further research was determined.


2020 ◽  
Vol 22 (100) ◽  
pp. 54-59
Author(s):  
S. О. Slobodian ◽  
B. V. Gutyj ◽  
D. F. Gufriy ◽  
P. S. Hnativ ◽  
S. D. Murska

In the conditions of modern technogenic pollution of the environment, environmental problems, improving the quality of livestock products and their food safety is an important and urgent issue today. Pollution of the environment with heavy metals due to man-made activities of the population has led to a number of problems of agricultural production. The aim of the study was to investigate the effect of sodium selenite and feed additive “Metisevit plus” on the protein-synthesizing function and functional state of the liver of rats in lead-cadmium intoxication. It was found that the level of total protein in the blood of rats exposed to cadmium and lead was reduced. These changes were due to a decrease in albumin levels and an increase in globulin levels. Thus, hypoproteinemia resulting from cadmium and lead poisoning may indicate impaired hepatic protein synthesis. Transamination enzymes play a major role in the relationship between protein and carbohydrate metabolism, which is important for switching the oxidation of energy substrates from glucose to amino acid energy nutrition, which is extremely important under the influence of various environmental factors. The most active transaminases are aspartate aminotransferase and alanine aminotransferase. In the study of the activity of aminotransferases in the serum of rats treated with lead acetate and cadmium chloride, an increase in the activity of enzymes in all periods of the study. The highest activity of aminotransferases was on days 21 and 28 of the experiment in the control group of rats, which carried out the combined load of cadmium and lead. Thus, the load of the body of animals with cadmium and lead contributes to the violation of the functional state and protein-synthesizing function of the liver of animals of the experimental groups. With the introduction of sodium selenite into the body of rats, the activity of ALT and AST in the serum of the first experimental group was slightly reduced compared with the control group. However, the activity of these enzymes did not reach physiological values. When setting the feed additive “Metisevit plus” throughout the experiment, it was found that the activity of aminotransferases in the serum of rats of the second experimental group ranged within physiological values. This is due to the hepatoprotective effect of the components of the feed additive “Metisevit Plus”. In the study of protein-synthesizing function of rat liver in experimental lead-cadmium toxicosis, it was found that after application to rats of the first experimental group of sodium selenite on days 14, 21 and 28, the level of total protein in serum gradually increased, but did not reach physiological values. Using the feed supplement “Metisevit plus” rats of the second experimental group, found a slightly higher level of total protein in their blood, which on the 28th day of the experiment reached physiological values and, accordingly, amounted to 66.8 ± 1.99 g/l. It should be noted that when administering the feed additive “Metisevit plus” protein-synthesizing liver function and liver function of rats of the second experimental group were restored faster than when administering sodium selenite to the first experimental group.


Author(s):  
O. V. Yaremko ◽  
R. A. Peleno

The effect of pyridoxine hydrochloride on the body mass of animals, absolute and average daily increment, relative growth rate, growth factor of live weight and multiplicity of increase in live weight of calves in the early stages of postnatal ontogenesis were studied. For the experiment, six groups of one-day calves (control and five experimental) were formed, with 5 animals in each, which, in addition to the main diet (MD), fed pyridoxine hydrochloride in different doses, according to the scheme: control group – MD, I experimental group – MD + 1 mg/kg g. m pyridoxine hydrochloride, II – MD + 2 mg/kg g. m of the preparation, ІІІ – MD + 3 mg/kg of w. m., IV – MD + 4 mg/kg g. m and V experimental group – MD + 5 mg/kg g. m pyridoxine hydrochloride. Conditionally, the research was divided into four periods: colostrum – from birth and up to 5 days, until the formation of digestion – from 5 to 21 days, the period of the formation of digestion scar – from 21 to 60 days and the end of the milk period – from 60 to 90 days. It was established that feeding to calves with pyridoxine hydrochloride resulted in an increase in the live weight of 60 days of calves by 5.9% in the second group (P < 0.05), by 8.7% in the third group, by 8.3% in IV and 9.2% in V groups (P < 0.001) and for 90 days it was higher by 9.3%, 9.1%, 9.2% and 9.1% respectively (P < 0.05). The highest average daily increments were in the colostrum period in calves IV and V groups, which were added to the main diet, respectively, 4 and 5 mg/kg b.w. pyridoxine hydrochloride, in the period prior to the formation of digestion scar in calves of group IV, in the period of the formation of digestion scar in calves of the III group and at the end of the milking period in calves of group V. Absolute incresements of calves in the colostrum period and the period prior to the formation of digestion scar were the highest in calves of Group IV and the difference in control was respectively 0.34 and 1.05 kg. During the period of the digestion scar formation and the end of the milk period, the highest absolute increases were in calves of group V, which was added to the main ration of 5 mg/kg b.w. pyridoxine hydrochloride. The highest increase in body weight of calves in colostric period and prior to the operation scar rate was in group IV, the period of digestion scar formation was in the third group and during the completion period of milk in the second group of calves. In colostric period the increase of live weight was higher in calves III, IV and V groups of 5 to 21 days in calves of group IV, from 21 to 60 days in the third group and during the completion period of milk in the second group of calves.


Author(s):  
P. О. Zaika ◽  
◽  
O. V. Kantemir ◽  

Dogs play a significant role in human life. It is difficult to list all the various aspects of their application. But along with the positive qualities of using dogs is the problem of regulating the numbers of stray animals. Among the agents that stimulate the processes of repair and regeneration, increase the basic physiological mechanisms of protection of the body are humic preparations such as sodium humate. Taking into account that preparations of natural origin have a special influence on the inflammatory process, it is necessary to find and study the possibilities of using biological preparations of humic acids. Six animals were selected for the detection of the effect of sodium humate on dogs by analogues. Castration was carried out in an open manner according to conventional methods After surgery, the animals were subcutaneously injected with a 0.1% solution of sodium humate at a dose of 0.1 ml per 1 kg of body weight, and the control saline solution once a day for 14 days. Examination of wounds and thermometry was performed twice daily for 16 days. Castration wounds were treated with antibacterial aerosol once daily. No additional medication was used. In the post-castration period, all operated dogs did not observe any bleeding, loss of the common vaginal sheath, intestine, cult of the seminal cord, or other complications. Since the third day in the general condition of the animals of the experimental group significant changes were observed, which were expressed in the attenuation of the signs of inflammation, which was manifested in the reduction of hyperemia of the wound edges and their edema. The tenderness of the scrotal tissue was noted, and the scrotal skin became folded. In control animals the development of the inflammatory process was more pronounced and occurred 2 to 3 days later than in the experimental animals. On the third day after surgery, the control animals showed clinical signs characteristic of acute inflammation: hyperemia, sharp pain and swelling of the scrotal tissues, elevated local temperature. In two animals of the control group swelling was spread beyond the scrotum - a slight swelling of the prepuce was noted. These signs were manifested against the background of general depression and decreased appetite. Recovery occurred on day 12 in the experimental group and on day 16 in the control one.


Author(s):  
V. Mykytyuk ◽  
T. Vasilenko ◽  
O. Orischuk ◽  
S. Tsap ◽  
I. Porotikova

The results of studies on the effect of various doses of cobalt with an optimized level of sulfur in rations of Askanian meat-and-wool ewes on digestibility of nutrients of consumed feed and digestibility of the studied mineral elements are presented. It was established that the level of nutrient digestibility of rations was higher in ewes of the experimental groups, which, to the generally accepted norm, were additionally injected with cobalt chloride in the amount of 10 %, which was equal 0.825 mg, 20 % – 0.900 mg, and 30 % – 0.975 mg per head per day . As a result of the conducted research, it was found that the ewes of the second experimental group differed in the highest indices of digestibility of nutrients of the ration, which increased the rate of cobalt by 10 %. The additional introduction of 20 % and 30 % cobalt to the basic ration did not contribute to a further increase in the digestibility factors of nutrients in the body of ewes of the experimental groups. The research has shown that the uptake of cobalt by experimental ewes increased as this element increased in the rations. Thus, 0.31 mg of cobalt was assimilated by the ewes of the second experimental group, which is 0.06 mg or 20.0 % higher compared to the control group; to the third-group of ewes by 0.07 mg or 28.0 % and to the fourth-group ewes by 0,08 mg or 32.0 % compared with analogues of the control group. However, when calculating these indicators in relative terms, it was established that the highest percentage of cobalt absorbed from that received was in the ewes of the second experimental group. Their advantage relative to the control group was 4.60 % (P <0.01), and III and IV experimental groups – 2.11 and 0.19 absolute percent.


Author(s):  
А.С. КУЗНЕЦОВ ◽  
Е.Л. ХАРИТОНОВ ◽  
К.С. ОСТРЕНКО ◽  
А.Н. ОВЧАРОВА

Изучен метаболизм азотсодержащих соединений в организме телят-молочников и бычков на откорме при включении в рационы кормовой добавки N-карбомилглутамат (NCG). Введение в основной рацион NCG приводит к снижению уровня свободного аммиака в плазме крови телят-молочников в 2 раза относительно контрольной группы. У молодняка на откорме в опытных группах концентрация аммиака в плазме крови была меньше на 20,9% в 1 группе и на 54,2% во II группе. При этом в опытной группе телят-молочников зафиксировано повышение прироста массы тела на 5,01% по сравнению с контролем. Введение кормовой добавки NCG способствовало переводу токсичного аммиака из плазмы крови, вследствие активизации орнитинового цикла в клетках печени и стенке рубца с образованием избытка карбомоилфосфата и синтеза эндогенного аргинина. Экзогенный аргинин из корма может быть заменен на NCG в рационе у жвачных животных. Добавление NCG в корм или ЗЦМ позволит улучшить утилизацию аммиака, повысить эффективность использования белка рациона, устойчивость к стрессам и будет являться фактором повышения продуктивности молодняка крупного рогатого скота. The influence of the metabolism of nitrogen-containing compounds in the body of dairy calves and calves on fattening was studied when the feed additive N-carbomylglutamate (NCG) was included in the diets. The introduction of the NCG feed supplement into the main diet leads to a decrease in the level of free ammonia in the blood plasma of dairy calves by 2 times compared to the control group. In fattened calves in the experimental groups, the concentration of ammonia in the blood plasma was less by 20.9% in group 1 and by 54.2% in group II. At the same time, in the experimental group of dairy calves, an increase in body weight gain was recorded by 5.01% compared to the control. The introduction of the feed additive NSP contributed to the transfer of toxic ammonia from the blood plasma, due to the activation of the ornithine cycle in the liver cells and the scar wall with the formation of an excess of carbomoylphosphate and the synthesis of endogenous arginine. Exogenous arginine from feed can be replaced with NCG in the diet of ruminants. The addition of NCG to feed or ZCM will improve the utilization of ammonia, increase the efficiency of the use of protein in the diet, resistance to stress, and will be a factor in increasing the productivity of young cattle.


2008 ◽  
Vol 62 (1-2) ◽  
pp. 13-24
Author(s):  
Horea Samanc ◽  
Danijela Kirovski ◽  
Milijan Jovanovic ◽  
Ivan Vujanac ◽  
Radisa Prodanovic ◽  
...  

The objective of these investigations was to examine possibilities for preventing fatty liver in cows during the peripartal period using the preparation HEPARENOL which includes methionine, lysine and choline in its composition. Investigations were performed on cattle of the Holstein breed whose average production during their last lactation was 8120 liters. The cows included in the experiment were divided into three groups of 10 cows each. Cows of the first group were administered the preparation HEPARENOL perorally 5-7 days before partus in daily doses of 50 ml per animal, and then daily during the first seven days after parturition in daily doses of 100 ml per animal. The second group of cows were administered the preparation in the same way as the first group, but they also received vitamin C injected deep i.m. in doses of 1000 mg per animal per day. The third group of cows were not exposed to any treatment and served as the control group. The body condition of the cows was marked according to the system Elanco Animal Health Bulletin Al 8478. Blood samples were taken by puncture of the jugular vein before the beginning of treatment, and on the third and 12th days after partus. The glucose concentration was determined in the blood samples. On the 12th day after partus, liver tissue samples were taken by biopsy for pathohistological examinations. Since the cows were fed in the same way and were in the same phase of the production-reproduction cycle, the average score of the body condition before treatment was approximately the same. However, on the 3rd day after partus, cows of the second experimental group showed average values that were significantly lower in comparison with the other two groups of cows. It is very interesting that a significantly lower value of glycemia (x=1.93?0.34 mmol/l) was established in the same group of cows at this time. In cows of the first experimental and the control group, glycemia was approximately the same during the examined period. In cows of the second experimental group on the 12th day after partus the glucose concentration significantly increased (x=2.42?0.58 mmol/l) against the values obtained for the 3rd day, but it was still significantly lower in comparison with the glycemia of cows of the first experimental (x=3.34?0.61 mmol/l) and the control group (x=3.48?0.49 mmol/l). Practically, throughout the entire period glycemia was statistically significantly lower in the second experimental group in comparison with the other two groups of cows. The degree of fatty liver on the 12th day postpartally, or five days after the end of treatment, was the lowest in cows of the second experimental group. In order to stress the differences, it is pointed out that there were two cows without fatty liver in the second group, while there were no such cows in the first experimental or in the control group. This group contained the smallest number of cows with a mean degree of fatty liver (n=2), while two cows had very strong fatty liver. There were no differences between the control and the first experimental group in the number of cows with a mean degree of fatty liver, with the exception that the control group had two and the first experimental group one cow with a mild degree of fatty liver. It seems that treatment using the preparation HEPARENOL, or methionine, lysine and choline, before and after parturition did not have a significant effect on the incidence and degree of fatty liver. Only cows with additional and simultaneous treatment with vitamin C had a lower incidence and degree of morphological changes in the liver, in addition to the sigfnificant changes in body condition and the blood glucose concentration.


2020 ◽  
Vol 22 (2) ◽  
pp. 15-19
Author(s):  
R. D. Ostapiv ◽  
◽  
O. I. Lukashchuk ◽  
V. Ya. Samaryk ◽  
M. I. Nagornyak ◽  
...  

The aim of the study was to test the ability of dextrin particles of different sizes, modified with N-stearylglutamic acid, to increase the transport of taurine into the blood plasma of rats. To achieve this goal, Wistar rats weighing 240–260 g were divided into control and four experimental groups. The control group was administered once perorally with water. The first experimental group was administered perorally with an aqueous solution of taurine at a dose of 100 mg/kg, the second and third ― a solution of taurine-dextrin associate with particle sizes of 60–90 nm and 500–800 nm, in which the dose of taurine was 100 mg/kg. The fourth experimental group (IV) was administered only dextrin. In 30 min, the rats were decapitated, and blood was collected. Blood cells and proteins were precipitated, and samples were derivatized with 1-fluoro-2,4-dinitrobenzene. Free amino acids of plasma were separated on a Dionex Ultimate 3000 liquid chromatograph equipped with a spectrophotometric detector, chromatographic column Luna C18 (2) 250×4.6 mm, elution type was gradient. It was registered that in the first and third experimental groups the content of taurine in the blood plasma of rats was 33.8% higher than in the control. Oral administration of taurine-dextrin associate with 60–90 nm particles increases the taurine content by 62.6% compared to the control and by 21.6% more than in the first and third experimental groups. This indicates efficient transport of taurine by dextrin only with particles of 60–90 nm. Administration of both taurine and taurine-dextrin associate reduced concentrations of glutamine, methionine, lysine, alanine, phenylalanine, and histidine. Moreover, the reason for the decrease in the content of the last two amino acids was not only taurine, but also the transporter itself. With the administration of the transporter the concentration of asparagine was lower than in control, and the content of arginine, on the contrary, higher. In the first experimental group, the concentration of aspartic acid and serine decreased, and in the second ― valine, leucine and tryptophan, in the third ― glutamic acid. The glycine content increased in the second experimental group. Ornithine content in all experimental groups except the third was higher, compared with the control. Thus, modified dextrin with a particle size of 60–90 nm increases the transport of taurine into the blood.


2018 ◽  
Vol 20 (92) ◽  
pp. 190-196 ◽  
Author(s):  
A. I. Ivanitskaya ◽  
Ya. V. Lesyk

The aim of the study was to find out the effect of casting of citrate nanosіlicon absorption and metasilicate sodium on the hematological parameters and lipid content and their fractional composition in blood plasma preparatory period 10 days prior to insemination and 20 days of lactation. The research was carried out on 60 rabbits of the Hyla hybrid, divided into three groups (control and two experimental), with 20 animals in each. The control animals were fed without limitation full-grain granulated feed with free access to water. Animals of the first experimental group (E-I) fed the diet of the control group and during the course of the day poured out the silicon citrate obtained using the nanotechnology method, 50 mkg Si/kg body weight with water. Samples of the second experimental group (E-II) feed the diet of the control group and set the sodium metasilicate (Na2SiO3H2O) 2.5 mg Si/kg body weight with water. The trial lasted 95 days, including the preparatory period of 10 days, the trial – 85 days. In the preparatory period of 10 days from the beginning of the study and in a trial of 20 days of lactation (65 days of supplements) in the rabbits, samples of blood from the marginal ear vein were taken. Hematologic studies were performed using the automatic hematologic analyzer “Orphee Mythic 18”, in the blood plasma, the content of total lipids and their fractional composition were determined. It was established that the number of erythrocytes and hemoglobin concentration in the blood of the rabbits of experimental group, which were given silicon citrate, were respectively higher by 15.2% (P < 0.05) and 12.0% (P < 0.01) by 20 days lactation versus control. This may indicate a more pronounced effect of the organic compound of silicon on the hematopoietic function of the body of the rabbits. According to white blood, the differences from control were found during the presentation of supplements with higher (P < 0.05) changes in 20 days of lactation in animal blood and experimental group. It was noted that the content of triacylglycerols in plasma of blood of groups I and II was lower by 31.2 and 32.8%, respectively (P < 0.05) compared to control. In blood plasma of animals in experimental groups an increase in the fractions of phosphatidylcholine, sphingomyelin and lysolecetin was observed compared to control. Such a redistribution was due to the reduction of the fractions of phosphatidic acid, phosphatidyl ethanolamine and phosphatidylinositol, and apparently, is associated with changes in the activity of the enzymes concerned. Hematological studies and determination of the fractional composition of lipids and phospholipids of blood plasma of the rabbits show positive changes that contribute to the metabolic accumulation of energy and plastic components of their organism.


2021 ◽  
Vol 6 (1) ◽  
pp. 345-351
Author(s):  
I. Baraliuc ◽  
◽  
V. Manolachi ◽  
A. Polevaia-Secareanu ◽  
A. Cotorcea

The purpose of the study was to identify the particularities of psychomotor abilities in 8-9 years old sambo athletes in the process of initial sport selection. Material and methods. The research presents an algorithm of the leading factors of the step-by-step selection of 165 8-9-year-old pupils for sambo wrestling. The first stage of selection was a thorough checkup, which determined that more than 50 % of the total number of pupils had disturbance of morphology and functionality of different body systems, there remained 68 examinees. At the third stage of selection the identification of intellectual abilities showed that only 73.53 % of pupils out of 68 (i.e. 50 pupils) had an average level of attention stability. According to the appreciation of psychomotor abilities of pupils we identified a decrease on the examinees number with 20 % out of 50 pupils. At the special stage of selection 17.5 % of 8-9 years old pupils, in 70 % of cases (i.e. for the majority of tests) failed to execute the requirements for motor abilities and coordination capacity. The basic experiment was elaborated and realized on the basis of an yearlong pedagogical experiment for which we organized two experimental study groups of sambo wrestlers in their first year of sport training: control group, that included 15 pupils that did not undergo any special selection, and the experimental group, consisting of 15 8-9 years old pupils that were specially selected. We created identical conditions for the learning and training process throughout a year for both groups. However, only in the experimental group we included the experimental methodology of sport diagnostics that contained a complex of scientifically based criteria and conditions. Results and discussion. During the experiment we identified the indices of “timely” reaction of the sambo wrestlers in the control group which improved only by 6 %, compared to the experimental group (57% more compared to the indices at the beginning of the experiment). It presented the dynamics of the tapping test of the sambo wrestlers in the control and experimental groups, from the first to the fourth period. The dynamics showed the local tiredness of the nervous and muscular system of the body, caused by the depression of their brain motor centers, which increased in both groups, but had a visible tendency of aggravation to the fourth period in the control group (from the first to the fourth period – within the limits of 62.80 %) compared to the experimental group, where the speed of elementary movements of children in the tapping test decreased inconclusively in every time period. Thus, at the end of the second period the results of the experimental group decreased by 11.25 %, to the end of the third period they lowered by 36.62 %, to the end of the fourth period they decreased by 15.55 % of the data of the previous period, which in conclusion comprised the decrease from the first to the fourth period up to 63.42 %. Conclusion. Thus, the veridically relevant situation of psychomotor development in the experimental group of sambo wrestlers, in our opinion, became possible due to their preliminary readiness (selection results) and the active experimental training action on them


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