Roles of Epithelial and Mesenchymal TRP Channels in Mediating Inflammatory Fibrosis

The maintenance of normal vision is dependent on preserving corneal transparency. For this to occur, this tissue must remain avascular and its stromal architecture needs to be retained. Epithelial transparency is maintained provided the uppermost stratified layers of this tissue are composed of terminally differentiated non-keratinizing cells. In addition, it is essential that the underlying stromal connective tissue remains avascular and scar-free. Keratocytes are the source of fibroblasts that are interspersed within the collagenous framework and the extracellular matrix. In addition, there are sensory nerve fibers whose lineage is possibly either neural crest or mesenchymal. Corneal wound healing studies have been undertaken to delineate the underlying pathogenic responses that result in the development of opacification following chemical injury. An alkali burn is one type of injury that can result in severe and long- lasting losses in ocular transparency. During the subsequent wound healing process, numerous different proinflammatory cytokines and proteolytic enzymes undergo upregulation. Such increases in their expression levels induce maladaptive expression of sustained stromal inflammatory fibrosis, neovascularization, and losses in the smooth optical properties of the corneal outer surface. It is becoming apparent that different transient receptor potential channel (TRP) isoforms are important players in mediating these different events underlying the wound healing process since injury upregulates both their expression levels and functional involvement. In this review, we focus on the involvement of TRPV1, TRPA1 and TRPV4 in mediating some of the responses that underlie the control of anterior ocular tissue homeostasis under normal and pathological conditions. They are expressed on both different cell types throughout this tissue and also on corneal sensory nerve endings. Their roles have been extensively studied as sensors and transducers of environmental stimuli resulting from exposure to intrinsic modulators and extrinsic ligands. These triggers include alteration of the ambient temperature and mechanical stress, etc., that can induce pathophysiological responses underlying losses in tissue transparency activated by wound healing in mice losses in tissue transparency. In this article, experimental findings are reviewed about the role of injury-induced TRP channel activation in mediating inflammatory fibrotic responses during wound healing in mice.

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HUBUNGAN MOBILISASI DINI TERHADAP PENYEMBUHAN LUKA POST OPERASI SECTIO CESARIA

Jurnal Penelitian Keperawatan Medik ◽

10.36656/jpkm.v2i1.187 ◽

2019 ◽

Vol 2 (1) ◽

pp. 14-17

Author(s):

Nurul Aini Siagian ◽

Syafira Nusaibah ◽

Andayani Boang Manalu

Keyword(s):

Wound Healing ◽

Healing Process ◽

Early Mobilization ◽

Sectio Caesarea ◽

Wound Healing Process ◽

P Value ◽

Cross Sectional ◽

Care Services ◽

Healing Wound ◽

Rapid Healing

Early mobilization includes factors that can affect the process of wound healing after surgery. Immediate mobilization in stages is very useful for the process of healing wounds and preventing infection and venous thrombosis. The purpose of this study was to determine whether there is a relationship between early mobilization and the process of healing wound post operative sectio caesarea at Sinar Husni General Hospital Medan Helvetia. The research design used was analytic survey with cross sectional approach. The sample in this study used the Consecutive Sampling method of data collection using a checklist sheet conducted on a sample of 19 respondents. The results of the study in this study are the majority of respondents who did early mobilization and who experienced rapid wound healing process as many as 4 people (21%) while the minority of respondents who did early mobilization and who experienced slow wound healing process were 1 person (5.3%). The majority of respondents who did not mobilize early and who experienced a slow wound healing process were 11 people (57.9%) and a minority who did not mobilize early and who experienced rapid healing as many as 4 people (21.1%). Statistical test results obtained p value = 0.046 <0.005. The conclusions of the results of this study indicate there is a relationship between early mobilization and the process of healing post operative sectio of caesarea. Suggestions The results of this study can be applied as a reference to improve nursing care services, especially in providing counseling and assistance to patients.

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Hubungan Status Gizi Dengan Proses Penyembuhan Luka Di Rsud H. Andi Sulthan Daeng Radja Kabupaten Bulukumba

Jurnal Kesehatan Panrita Husada ◽

10.37362/jkph.v2i1.147 ◽

2017 ◽

Vol 2 (1) ◽

pp. 20-28

Author(s):

Fitriani ◽

Tenriwati

Keyword(s):

Wound Healing ◽

Nutritional Status ◽

Healing Process ◽

Data Retrieval ◽

Observational Research ◽

Wound Healing Process ◽

P Value ◽

Chi Square ◽

Cross Sectional ◽

Chi Square Test

At present, the incidence of injuries in Indonesia is quite high, as seen from data on traffic accidents in the general public. There are several factors that affect wound healing, one of which is nutritional status. Based on the preliminary data retrieval conducted by researchers in the seruni surgery room in the last 1 month namely in March, it was found that the number of wounded patients was 109 patients, where the number of men was 78 (71.56%) while the number of women was 31 ( 28.44%) The purpose of this study was to determine the relationship between nutritional status and the wound healing process in RSUD. H. Andi. Sulthan Daeng Radja Kab. Bulukumba. This research uses the type of design of this research is quantitative research. This type of research uses analytic observational research with cross sectional approach. The sample of this study were 41 respondents taken by purposive sampling method. Data analysis in this study used the chi-square test (chi square test). The results of the analysis used the chi-square statistical test with a confidence level (α = 0.05). Based on the results of this test, the p value is 0.001, thus p <α (0.001 <0.05), then Ho is rejected and Ha is accepted. The conclusion of this study is that there is a relationship between nutritional status and the process of wound healing in RSUD. H. Andi. Sulthan Daeng Radja Kab. Bulukumba. Researchers suggest that this study be used as a material consideration in the fulfillment of nutrition in wound care patients in RSUD.H.A. Sulthan Daeng Radja Kab. Bulukumba and this research can be continued by conducting research related to the wound healing process and linking it with other variables.

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Natural, Synthetic and their Combinatorial Nanocarriers Based Drug Delivery System in the Treatment Paradigm for Wound Healing Via Dermal Targeting

Current Pharmaceutical Design ◽

10.2174/1381612826666200612164511 ◽

2020 ◽

Vol 26 (36) ◽

pp. 4551-4568

Author(s):

Mohammad Kashif Iqubal ◽

Sadaf Saleem ◽

Ashif Iqubal ◽

Aiswarya Chaudhuri ◽

Faheem Hyder Pottoo ◽

...

Keyword(s):

Drug Delivery ◽

Wound Healing ◽

Photodynamic Therapy ◽

Growth Factors ◽

Combination Therapy ◽

Healing Process ◽

Wound Healing Process ◽

Wound Site ◽

Synthetic Drugs ◽

Treatment Paradigm

A wound refers to the epithelial loss, accompanied by loss of muscle fibers collagen, nerves and bone instigated by surgery, trauma, frictions or by heat. Process of wound healing is a compounded activity of recovering the functional integrity of the damaged tissues. This process is mediated by various cytokines and growth factors usually liberated at the wound site. A plethora of herbal and synthetic drugs, as well as photodynamic therapy, is available to facilitate the process of wound healing. Generally, the systems used for the management of wounds tend to act through covering the ruptured site, reduce pain, inflammation, and prevent the invasion and growth of microorganisms. The available systems are, though, enough to meet these requirements, but the involvement of nanotechnology can ameliorate the performance of these protective coverings. In recent years, nano-based formulations have gained immense popularity among researchers for the wound healing process due to the enhanced benefits they offer over the conventional preparations. Hereupon, this review aims to cover the entire roadmap of wound healing, beginning from the molecular factors involved in the process, the various synthetic and herbal agents, and combination therapy available for the treatment and the current nano-based systems available for delivery through the topical route for wound healing.

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Scientific aspect on Diet explained by Susrutha in Wound Healing

Journal of Ayurveda and Integrated Medical Sciences (JAIMS) ◽

10.21760/jaims.v2i05.10268 ◽

2017 ◽

Vol 2 (05) ◽

Author(s):

Manoj Kumar ◽

Amareshappa . ◽

Anjali Bharadwaj ◽

Shailaja S. V.

Keyword(s):

Wound Healing ◽

Mineral Metabolism ◽

Healing Process ◽

Surgical Techniques ◽

The Body ◽

Wound Healing Process ◽

Human Being ◽

Different Sources ◽

Scientific Aspect ◽

Important Branch

Wound healing has been the burning problem in a surgical practice because of a remarkable increase in the number of traumatic cases. A wound causes a number of changes in the body that can affect the healing process, including changes in energy, protein, carbohydrate, fat, vitamin and mineral metabolism. Various Ayurveda literatures, particularly, Sushruta Samhita, which is said to be an ancient textbook of surgery in Ayurveda, has mentioned about the diet for the person suffering from the wound, and the author said that diet plays a very important role in the wound healing process. Sushruta - The father of surgery has scientifically classified it in a systemic manner, whose wealth of clinical material and the principles of management are valid even today. Shalya Tantra (surgical branch in Ayurveda Science) is one of the important branch of Ayurveda, in which surgical and para-surgical techniques has described for management of various diseases. Vrana is the most important and widely described chapter of Shalya Tantra. Vrana (wound) is one of them, which have been managed by human being from starting of civilization. Under the circumstances, the first thing which the men came across was the injury from different sources which caused him the Vrana. Vrana is seen as debilitating and scaring disorder, usually seen affecting the human being at any age. Well balanced nutrition plays an essential role in the wound healing.

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PENGARUH MOBILISASI DINI TERHADAP PENYEMBUHAN LUKA POST SECTIO CAESAREA

Jurnal IPTEKS Terapan ◽

10.22216/jit.2017.v11i1.661 ◽

2017 ◽

Vol 11 (1) ◽

pp. 109

Author(s):

Reni Heryani ◽

Ardenny Denny

Keyword(s):

Wound Healing ◽

Placenta Previa ◽

Healing Process ◽

Early Mobilization ◽

Sectio Caesarea ◽

Uterine Action ◽

Wound Healing Process ◽

P Value ◽

Post Test ◽

Development Goals

ABSTRAK Salah satu tujuan pembangunan era Millenium Development goals (MDG’s) 2015 adalah perbaikan kesehatan maternal. Salah satu cara di dalam persalinan atau mengeluarkan bayi secara patologis yaitu dengan sectio caesarea. Menurut statistik 3.509 kasus sectio caesarea, indikasi terbanyak adalah disproporsi cephalo pelvik (21%), sedangkan indikasi lain adalah gawat janin (14%), plasenta previa (11%), pernah sectio caesarea (11%), incoordinate uterine action (9%), preeklamsi dan hipertensi (7%). Penelitian ini menggunakan desain penelitian Quasy Experiment dengan post test only with control. Hasil penelitian menunjukkan bahwa umumnya responden memiliki umur tidak berisiko terhadap tindakan operasi yati sebanyak 15 orang (75%), sebagian responden pertama kali dilakukan operasi caesarea yaitu sebanyak 10 orang (50,0%), sebagian responden mengalami penyembuhan luka yang normal yaitu sebanyak 13 orang (65,0%), dan sebagian responden responden melakukan mobilisasi dini yaitu sebanyak 14 orang (70,0%). Secara statistik terdapat pengaruh mobilisasi dini terhadap penyembuhan luka post sectio caesarea di RSUD Arifin Achmad Pekanbaru (p value = 0.007). Disarankan bagi respnden dapat meningkatkan kesadarannya dalam meningkatkan derajat kesehatan melalui latihan mobilisasi dini untuk mempercepat proses penyembuhan luka akibat operasi. Kata Kunci : sectio caesarea, mobilisasi, penyembuhan luka ABSTRACT One of the goals of development era of Millennium Development Goals (MDG's) in 2015 is the improvement of maternal health. One way in the delivery or the baby out with the pathological ie sectio caesarea. According to 3509 statistics compiled case sectio caesarea, an indication of cephalo pelvic disproportion is the highest (21%), while the other is an indication of fetal distress (14%), placenta previa (11%), never sectio caesarea (11%), incoordinate uterine action (9%), preeclampsia and hypertension (7%). This study research design quasy Experiment with post test only with control. The results showed that respondents generally have a lifespan of no risk for surgery yati many as 15 people (75%), the majority of respondents were first performed caesarea operation as many as 10 people (50.0%), the majority of respondents experienced a normal wound healing as many as 13 people (65.0%), and the majority of respondents respondents did early mobilization as many as 14 people (70.0%). There is a statistically significant relationship between early mobilization on wound healing post sectio caesarea (p value = 0.007). Suggested for respnden can increase awareness in improving health status through early mobilization exercises to accelerate the wound healing process as a result of the operation. Keywords: sectio caesarea, mobilization, wound healing

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Development of a Topical Insulin Polymeric Nanoformulation for Skin Burn Regeneration: An Experimental Approach

International Journal of Molecular Sciences ◽

10.3390/ijms22084087 ◽

2021 ◽

Vol 22 (8) ◽

pp. 4087

Author(s):

Maria Quitério ◽

Sandra Simões ◽

Andreia Ascenso ◽

Manuela Carvalheiro ◽

Ana Paula Leandro ◽

...

Keyword(s):

Wound Healing ◽

Healing Process ◽

In Vitro Release ◽

Peptide Hormone ◽

Plga Nanoparticles ◽

Wound Healing Process ◽

Target Area ◽

Encapsulation Process

Insulin is a peptide hormone with many physiological functions, besides its use in diabetes treatment. An important role of insulin is related to the wound healing process—however, insulin itself is too sensitive to the external environment requiring the protective of a nanocarrier. Polymer-based nanoparticles can protect, deliver, and retain the protein in the target area. This study aims to produce and characterize a topical treatment for wound healing consisting of insulin-loaded poly-DL-lactide/glycolide (PLGA) nanoparticles. Insulin-loaded nanoparticles present a mean size of approximately 500 nm and neutral surface charge. Spherical shaped nanoparticles are observed by scanning electron microscopy and confirmed by atomic force microscopy. SDS-PAGE and circular dichroism analysis demonstrated that insulin preserved its integrity and secondary structure after the encapsulation process. In vitro release studies suggested a controlled release profile. Safety of the formulation was confirmed using cell lines, and cell viability was concentration and time-dependent. Preliminary safety in vivo assays also revealed promising results.

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Wound Healing Promotion by Hyaluronic Acid: Effect of Molecular Weight on Gene Expression and In Vivo Wound Closure

Pharmaceuticals ◽

10.3390/ph14040301 ◽

2021 ◽

Vol 14 (4) ◽

pp. 301

Author(s):

Yayoi Kawano ◽

Viorica Patrulea ◽

Emmanuelle Sublet ◽

Gerrit Borchard ◽

Takuya Iyoda ◽

...

Keyword(s):

Gene Expression ◽

Molecular Weight ◽

Wound Healing ◽

Hyaluronic Acid ◽

Healing Process ◽

Dermal Fibroblasts ◽

Water Soluble ◽

Wound Healing Process ◽

And Migration ◽

Proliferation And Migration

Hyaluronic acid (HA) has been known to play an important role in wound healing process. However, the effect of molecular weight (MW) of exogenously administered HA on the wound healing process has not been fully understood. In this study, we investigated HA with different MWs on wound healing process using human epidermal keratinocytes and dermal fibroblasts. Cell proliferation and migration ability were assessed by water soluble tetrazolium (WST) assay and wound scratch assay. We examined the effect of HA addition in a full-thickness wound model in mice and the gene expression related to wound healing. Proliferation and migration of HaCaT cells increased with the increase of MW and concentration of HA. Interleukin (IL-1β), IL-8 and vascular endothelial growth factor (VEGF) as well as matrix metalloproteinase (MMP)-9 and MMP-13 were significantly upregulated by high molecular weight (HMW) HA in keratinocytes. Together with VEGF upregulation and the observed promotion of HaCaT migration, HA with the MW of 2290 kDa may hold potential to improve re-epithelialization, a critical obstacle to heal chronic wounds.

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Role of glyoxalases in wound healing process: an in vitro study

Free Radical Biology and Medicine ◽

10.1016/j.freeradbiomed.2020.12.366 ◽

2021 ◽

Vol 165 ◽

pp. 39

Author(s):

Francesca Lombardi ◽

Silvano Santini ◽

Paola Palumbo ◽

Valeria Cordone ◽

Virginio Bignotti ◽

...

Keyword(s):

Wound Healing ◽

Healing Process ◽

In Vitro Study ◽

Vitro Study ◽

Wound Healing Process

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N-Acetylcysteine Added to Local Anesthesia Reduces Scar Area and Width in Early Wound Healing—An Animal Model Study

International Journal of Molecular Sciences ◽

10.3390/ijms22147549 ◽

2021 ◽

Vol 22 (14) ◽

pp. 7549

Author(s):

Wiktor Paskal ◽

Adriana M. Paskal ◽

Piotr Pietruski ◽

Albert Stachura ◽

Kacper Pełka ◽

...

Keyword(s):

Wound Healing ◽

Early Stage ◽

Healing Process ◽

Wound Healing Process ◽

Sprague Dawley ◽

Time Points ◽

Model Study ◽

Sprague Dawley Rats ◽

Animal Model Study ◽

Anesthetic Solution

The aim of the study was to evaluate if a pre-incisional N-acetylcysteine (NAC) treatment altered the process of wound healing in a rat model. The dorsal skin of 24 Sprague-Dawley rats was incised in six locations. Before the incisions were made, skin was injected either with lidocaine and epinephrine (one side) or with these agents supplemented with 0.015%, 0.03%, or 0.045% NAC (contralaterally). Photographic documentation of the wound healing process was made at 11 time points. Rats were sacrificed 3, 7, 14, or 60 days after incision to excise scars for histological analysis. They included: Abramov scale scoring, histomorphometry analysis, and collagen fiber arrangement assessment. Skin pretreated with 0.03% NAC produced the shortest scars at all analyzed time points, though this result was statistically insignificant. At this NAC concentration the scars had smaller areas on the third day and were narrower on the day 4 compared with all the other groups (p < 0.05). On day 7, at the same concentration of NAC, the scars had a higher superficial concentration index (p = 0.03) and larger dermal proliferation area (p = 0.04). NAC addition to pre-incisional anesthetic solution decreased wound size and width at an early stage of scar formation at all concentrations; however, with optimal results at 0.03% concentration.

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Multi-Functional Core-Shell Nanofibers for Wound Healing

Nanomaterials ◽

10.3390/nano11061546 ◽

2021 ◽

Vol 11 (6) ◽

pp. 1546

Author(s):

Zhen Li ◽

Shunqi Mei ◽

Yajie Dong ◽

Fenghua She ◽

Puwang Li ◽

...

Keyword(s):

Wound Healing ◽

Growth Factors ◽

Drug Release ◽

Release Rate ◽

Healing Process ◽

Wound Healing Process ◽

Core Shell ◽

High Production Rate ◽

Centrifugal Spinning ◽

Multiple Drugs

Core-shell nanofibers have great potential for bio-medical applications such as wound healing dressings where multiple drugs and growth factors are expected to be delivered at different healing phases. Compared to monoaxial nanofibers, core-shell nanofibers can control the drug release profile easier, providing sustainable and effective drugs and growth factors for wound healing. However, it is challenging to produce core-shell structured nanofibers with a high production rate at low energy consumption. Co-axial centrifugal spinning is an alternative method to address the above limitations to produce core-shell nanofibers effectively. In this study, a co-axial centrifugal spinning device was designed and assembled to produce core-shell nanofibers for controlling the release rate of ibuprofen and hEGF in inflammation and proliferation phases during the wound healing process. Core-shell structured nanofibers were confirmed by TEM. This work demonstrated that the co-axial centrifugal spinning is a high productivity process that can produce materials with a 3D environment mimicking natural tissue scaffold, and the specific drug can be loaded into different layers to control the drug release rate to improve the drug efficiency and promote wound healing.

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