scholarly journals Impact of Dietary or Drinking Water Ruminococcus sp. Supplementation and/or Heat Stress on Growth, Histopathology, and Bursal Gene Expression of Broilers

2021 ◽  
Vol 8 ◽  
Author(s):  
Adel Hassan Saad ◽  
Mohamed S. Ahmed ◽  
Mohamed Aboubakr ◽  
Hanan A. Ghoneim ◽  
Mohamed M. Abdel-Daim ◽  
...  
Keyword(s):  
Gene Expression ◽  
Drinking Water ◽  
Heat Stress ◽  
Serum Biochemistry ◽  
Broiler Chicks ◽  
Internal Organs ◽  
Formulated Diet ◽  
Serum Biochemical ◽  
Enzyme Supplementation ◽  
The Impact

This research was conducted to evaluate the impact of dietary or drinking water Ruminococcus sp. supplementation and/or heat stress (HS) on the growth, serum biochemistry, tissue antioxidant, phagocytic assay, histopathology, and bursa gene expression of broilers. Day-old broiler chicks were allotted into six groups according to HS and/or Ruminococcus with or without enzyme supplementation. The first group was the control one, with a formulated diet and normal environmental temperature but without any supplement. The second group fed on Ruminococcus-supplemented diet (1 kg/kg diet). The third group fed on a formulated diet without supplement, and Ruminococcus and digestive enzymes were given in drinking water (0.1 ml/L). The fourth one was the heat stress group, with a normal formulated diet. The fifth and the sixth groups served as second and third groups, respectively, but with heat stress. The results of this experiment indicated that thermal temperature negatively affected the parameters of growth performance, serum biochemical, tissue antioxidants, and phagocytic assay. Moreover, heat stress led to pathological lesions in the internal organs and affected the expression of some genes related to heat stress, including proapoptotic genes such as caspase8 and bax, inflammatory genes such as NF-κβ1, and heat shock protein such as HSP 70 in the bursal tissue. These bad effects and abnormalities were mitigated by Ruminococcus alone or with enzyme supplementation, which improved all the above-mentioned parameters.

scholarly journals Heat Stress Alters the Intestinal Microbiota and Metabolomic Profiles in Mice

2021 ◽  
Vol 12 ◽  
Author(s):  
Chaoyue Wen ◽  
Siyu Li ◽  
Jiaojiao Wang ◽  
Yimin Zhu ◽  
Xin Zong ◽  
...  
Keyword(s):  
Heat Stress ◽  
Fatty Acid ◽  
High Temperature ◽  
Intestinal Microbiota ◽  
High Throughput Sequencing ◽  
Acid Metabolism ◽  
Core Body Temperature ◽  
Control Group ◽  
Serum Biochemical ◽  
The Impact

BackgroundHeat stress has negative effects on the intestinal health of humans and animals. However, the impact of heat stress on intestinal microbial and metabolic changes remains elusive. Here, we investigated the cecal microbial and metabolic profiles in mice in response to heat stress.MethodsThe mouse heat stress model was constructed by simulating a high-temperature environment. Twenty mice were randomly assigned to two groups, the control group (CON, 25°C) and the heat treatment group (HS, 40°C from 13:00 to 15:00 every day for 7 days). Serum and cecal contents were collected from the mice for serum biochemical analysis, 16S rRNA high-throughput sequencing, and non-targeted metabolomics.ResultsBoth core body temperature and water intake were significantly increased in the HS group. Serum biochemical indicators were also affected, including significantly increased triglyceride and decreased low-density lipoprotein in the heat stress group. The composition and structure of intestinal microbiota were remarkably altered in the HS group. At the species level, the relative abundance of Candidatus Arthromitus sp. SFB-mouse-Japan and Lactobacillus murinus significantly reduced, while that of Lachnospiraceae bacterium 3-1 obviously increased after HS. Metabolomic analysis of the cecal contents clearly distinguished metabolite changes between the groups. The significantly different metabolites identified were mainly involved in the fatty acid synthesis, purine metabolism, fatty acid metabolism, cyanoamino acid metabolism, glyceride metabolism, and plasmalogen synthesis.ConclusionIn summary, high temperature disrupted the homeostatic balance of the intestinal microbiota in mice and also induced significant alterations in intestinal metabolites. This study provides a basis for treating intestinal disorders caused by elevated temperature in humans and animals and can further formulate nutritional countermeasures to reduce heat stress-induced damage.

scholarly journals Effects of zinc oxide nanoparticles on regulatory appetite and heat stress protein genes in broiler chickens subjected to heat stress

2020 ◽  
Vol 98 (10) ◽  
Author(s):  
Suriya Kumari Ramiah ◽  
Elmutaz Atta Awad ◽  
Nur Izzah Mohd Hemly ◽  
Mahdi Ebrahimi ◽  
Olubodun Joshua ◽  
...  
Keyword(s):  
Gene Expression ◽  
Zinc Oxide ◽  
Heat Stress ◽  
Zinc Oxide Nanoparticles ◽  
Broiler Chickens ◽  
Stress Proteins ◽  
Regulation Of Gene Expression ◽  
Basal Diet ◽  
Oxide Nanoparticles ◽  
Broiler Chicks

Abstract This study was conducted to explore the effect of the zinc oxide nanoparticles (ZnONPs) supplement on the regulatory appetite and heat stress (HS) genes in broiler chickens raised under high or normal ambient temperatures. In this study, 240 one-day-old male broiler chicks (Cobb 500) were randomly assigned to 48 battery cages. From day 1, these 48 cages were randomly subjected to four different treatment strategies: Control (wherein, their basal diet included 60 mg/kg of ZnO), ZNONPs 40 (wherein basal diet included 40 mg/kg of ZnONPs), ZnONPs 60 (basal diet included 60 mg/kg of ZnONPs), and ZnONPs 100 (basal diet included 100 mg/kg of ZnONPs). Thereafter, from day 22 to 42, the chickens from each dietary treatment group were subjected to different temperature stresses either normal (23 ± 1 °C constant) or HS (34 ± 1 °C for 6 h/d), which divided them into eight different treatment groups. Our findings revealed that dietary ZnONPs altered the gene expression of cholecystokinin (ileum), heat stress proteins (HSP) 70 (jejunum and ileum), and HSP 90 (duodenum, jejunum, and ileum). The gene expression of ghrelin was affected by the interaction between the ZnONPs concentration and temperature in the duodenum and stomach. More studies are required to elucidate its complex physiological and biochemical functions of the regulation of gene expression within the intestine in heat-stressed broiler chickens.

scholarly journals Splenic Gene Expression Signatures in Slow-Growing Chickens Stimulated in Ovo with Galactooligosaccharides and Challenged with Heat

Animals ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 474 ◽  
Author(s):  
Elzbieta Pietrzak ◽  
Aleksandra Dunislawska ◽  
Maria Siwek ◽  
Marco Zampiga ◽  
Federico Sirri ◽  
...  
Keyword(s):  
Gene Expression ◽  
Heat Stress ◽  
Broiler Chickens ◽  
Physiological Saline ◽  
Negative Effects ◽  
In Ovo ◽  
Stress Related Genes ◽  
The Impact ◽  
Slow Growing ◽  
Resistance To Heat

Galactooligosaccharides (GOS) that are delivered in ovo improve intestinal microbiota composition and mitigate the negative effects of heat stress in broiler chickens. Hubbard hybrids are slow-growing chickens with a high resistance to heat. In this paper, we determined the impact of GOS delivered in ovo on slow-growing chickens that are challenged with heat. The experiment was a 2 × 2 × 2 factorial design. On day 12 of incubation, GOS (3.5 mg/egg) was delivered into the egg (n = 300). Controls (C) were mock-injected with physiological saline (n = 300). After hatching, the GOS and C groups were split into thermal groups: thermoneutral (TN) and heat stress (HS). HS (30 °C) lasted for 14 days (days 36–50 post-hatching). The spleen (n = 8) was sampled after acute (8.5 h) and chronic (14 days) HS. The gene expression of immune-related (IL-2, IL-4, IL-6, IL-10, IL-12p40, and IL-17) and stress-related genes (HSP25, HSP90AA1, BAG3, CAT, and SOD) was detected with RT-qPCR. Chronic HS up-regulated the expression of the genes: IL-10, IL-12p40, SOD (p < 0.05), and CAT (p < 0.01). GOS delivered in ovo down-regulated IL-4 (acute p < 0.001; chronic p < 0.01), IL-12p40, CAT and SOD (chronic p < 0.05). The obtained results suggest that slow-growing hybrids are resistant to acute heat and tolerant to chronic heat, which can be supported with in ovo GOS administration.

scholarly journals Toxicological and gene expression analysis of the impact of aflatoxin B1 on hepatic function of male broiler chicks

2009 ◽  
Vol 88 (2) ◽  
pp. 360-371 ◽  
Author(s):  
L.P. Yarru ◽  
R.S. Settivari ◽  
E. Antoniou ◽  
D.R. Ledoux ◽  
G.E. Rottinghaus
Keyword(s):  
Gene Expression ◽  
Expression Analysis ◽  
Aflatoxin B1 ◽  
Gene Expression Analysis ◽  
Hepatic Function ◽  
Broiler Chicks ◽  
The Impact

scholarly journals Evaluation of different litter materials on haematogical and serum biochemical indices of intensively managed finisher broiler chickens

2020 ◽  
Vol 47 (3) ◽  
pp. 59-66
Author(s):  
H. O. Obikaonu ◽  
S. N. Obiora ◽  
P. Obiagwu
Keyword(s):  
Broiler Chickens ◽  
Serum Biochemistry ◽  
The Other ◽  
Serum Globulin ◽  
Rice Hulls ◽  
Wood Shavings ◽  
Serum Biochemical ◽  
Intensively Managed ◽  
Biochemical Indices ◽  
The Impact

Attempts made to improve broiler production had been focused more on feed with little or no consideration on the impact of litter quality which is a major concern in chicken production not only because it affects the flock health and productivity, but also because it may act as potential reservoir and transmission vehicle for pathogens and potential pathogens. Since blood is considered as an important material for the evaluation of health status in animals, there is need therefore, to investigate the impact of different litter materials on the hematological and serum biochemistry of broiler finisher chicken. A56-day feeding trial was conducted to evaulate the effect of different litter materials on the haematological and serum biochemical indices of intensively managed finisher broilers. Four different litter materials were used for the experiment viz wood, shaving, Groundnut hulls; coconut husks and rice hulls. Wood shavings was used as the control owing to the fact that it is the most commonly used litter in this zone. The coconut husks were manually shred to extract the fibres which was used while the other materials were used as sourced. A toal of 120 broiler birds were used for the experiment, they were divided into four treatments (T1= Wood shavings, T2= Groundnut hulls, T3=Coconut husks, and T4=Rice hulls) of 30 birds each and sub divided into 10 birds per replicate. Each replicate was housed in a pen fitted with broding facilities. Feed and water were given ad libilum for eight weeks. At the end of the experiment blood was collected from the birds, three per treatment given a total of 12 birds. They were analyzed for haematological and serum biochemical indices. Haemoglobin values compared favourably (P>0.05) with that of the control. PCVvalues of T2 and T3 compared favourably well with that of control but significant differences existed (P<0.05) between the PCV value of T4 and the other treatments. The lymphocytes and neutrophils were not affected by the bedding materials showing that the litter conditions did not adversely affect the health and welfare of the birds. Serum protein, serum albumin and serum globulin were not affected by treatements (P>0.05) resulting in better protein efficiency ratio recorded in all the treatments. Serum electolytes calcium and phosphorus levels recorded in this study shows no adverse effect on the integrity of the liver in boosting cataion/anaion exchange. The haematological and serum biochemical indices recorded in this study showed no notable health challenges on the birds.

scholarly journals Dietary vegetable oils inclusion on the performance, hormonal levels and hsp 70 gene expression in broilers under heat stress

2019 ◽  
Vol 42 ◽  
pp. e45517
Author(s):  
Ali Rafiei Tari ◽  
Ali Asghar Sadeghi ◽  
Seyed Naser Mousavi
Keyword(s):  
Gene Expression ◽  
Heat Stress ◽  
Olive Oil ◽  
Corn Oil ◽  
Linseed Oil ◽  
Broiler Chicks ◽  
Feed Conversion ◽  
Hsp 70 ◽  
Olive Oils ◽  
Hormonal Levels

The aim of the present study was to compare the effects of unsaturated and saturated oils on the performance, hormonal levels and hsp gene expression in broiler chickens exposed to heat stress. 300 one-day male broiler chicks were assigned to 4 treatments (Diets containing palm, corn, linseed or olive oils) with 5 replicates. At day 28 of age, 2 chickens were removed from each replicate, then blood samples and liver tissue samples were collected for analyses. Feeding linseed and olive oil reduced feed conversion ratio compared to corn and palm oils. The lowest level of insulin was for chickens fed linseed oil and corn oil. The highest level of corticosterone was found in chickens fed palm oil and the lowest level was for those received linseed oil. Chickens received linseed and corn oils had the highest levels of T3 and T4 and those fed palm and olive oils had the lowest levels. The highest HSP 70 gene expression was for chickens fed diet containing olive and linseed oils and the lowest one was for those fed corn and palm oils. It was concluded that olive oil and linseed oil could improve performance and heat tolerance of chickens under heat stress.

PSIX-25 Feed and water deprivation at weaning and subsequent heat stress impacts serum and ileal markers of gastrointestinal integrity in nursery pigs

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 496-497
Author(s):  
Nathan L Horn ◽  
Adrienne Woodward ◽  
Kola Ajuwon ◽  
Layi Adeola
Keyword(s):  
Gene Expression ◽  
Heat Stress ◽  
Water Deprivation ◽  
Intestinal Barrier ◽  
Serum Cortisol ◽  
Intestinal Barrier Function ◽  
Nursery Pigs ◽  
Stress Markers ◽  
Pig Performance ◽  
The Impact

Abstract Social and environmental stressors impact nursery pig performance and may be linked to gastrointestinal dysfunction. The current experiment was conducted to investigate the impact of feed and water deprivation on serum stress markers and ileal mucosal gene expression in nursery pigs. Mixed-sex pigs were allotted on the basis of IBW (7.0 kg ± 0.89) in a RCBD with treatments in a split-plot arrangement and consisting of the whole-plot factor of with or without a 24-h feed and water deprivation at weaning and the sub-plot factor of with or without a cyclic 3-d heat stress starting 27 d post-weaning. On 1, 27, and 30 d post-weaning one pig from each pen was selected, blood was collected for measurement of serum cortisol, corticotrophin releasing factor (CRF), and endotoxins, and an ileal mucosal scraping was taken and gene expression of claudin 1 (CL-1), occludin (OC), and zonula occludens 1 (ZO-1) were measured by RT PCR. There was an increase (P &lt; 0.05) in serum CRF and endotoxins and a tendency for an increase (P = 0.09) in serum cortisol due to the deprivation 1 d post-weaning. Further, there was a tendency for an increase (P &lt; 0.10) in serum endotoxins and CRF due to the deprivation at 27 and 30 d post-weaning, respectively. Gene expression of CL-1 tended to increase (P = 0.10), and OC decreased (P = 0.05) due to the deprivation 1 d post-weaning. Expression of the OC gene decreased (P &lt; 0.05) due to the deprivation 27 d post-weaning and OC and ZO-1 gene expression tended to decrease (P = 0.07) due to the heat stress 30 d post-weaning. These results show that post-weaning stress events alter serum stress markers and impact intestinal barrier function.

scholarly journals Impact of Multienzymes Dose Supplemented Continuously or Intermittently in Drinking Water on Growth Performance, Nutrient Digestibility, and Blood Constituents of Broiler Chickens

Animals ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 375
Author(s):  
Youssef Attia ◽  
Mahmoud El-kelawy ◽  
Mohammed Al-Harthi ◽  
Ali El-Shafey
Keyword(s):  
Drinking Water ◽  
Growth Performance ◽  
Body Weight Gain ◽  
Nutrient Digestibility ◽  
The Body ◽  
Broiler Chicks ◽  
Economic Traits ◽  
Production Index ◽  
Enzyme Supplementation ◽  
European Production

The aim of this work was to study the continuous or intermittent impact of a multienzyme supplement on growth performance, nutrient digestibility, and blood metabolites of broilers, and to evaluate production index of dietary supplementation. A total of 315 unsexed day-old Arbor Acres broiler chicks were randomly distributed to seven treatments groups, keeping initial body weights similar, in 35-floor pens (replicates) of nine chicks per replicate (pen) and five experimental units per treatment. All experimental groups were fed the same basal diet and administered seven multienzyme treatments: the 1st group (control) did not receive any enzyme supplementation; the 2nd, 3rd, and 4th groups were administered multienzymes at 0.5, 1.0, and 1.5 mL/L drinking water, respectively. Each enzyme supplemented-group was divided into two subgroups, with additives being applied either continuously (24 h/day) or intermittently (12 h/day) from 1 to 35 days old. Regardless of administration method, multienzyme supplements at 1.0 mL/L water along with a corn-soybean meal diet increased the body weight gain (BWG) by 7.8% compared to 0.5 mL/L water during days 1–21 of age. In addition, 1.5 mL/L water significantly improved BWG by 5.1% of broilers compared to 0.5 mL/L water during days 1–35 of age. Enzyme supplementation at 1.5 mL/L water significantly enhanced feed conversion ratio (FCR) by 4.3% during days 1–21 of age, and FCR by 5.2% and European production index (EPI) by 10.4% during days 1–35 of age compared to the group on 0.5 mL/L water. For the whole period, there were improvements of beneficial consideration in BWG (4.0%), FCR (4.0%), and European production index (8.2%) due to continuous multienzyme supplementation at 0.5 mL/L water compared to the same dose added intermittently. A similar trend was observed due to intermittent multienzymes at 1 mL/L drinking water that resulted in increased BWG by (6.4%) and improved FCR by (6.7%) and EPI by (12.7%). Intermittent administration significantly increased feed intake of broilers during 22–35 days of age compared to continuous supplementation. Multienzymes at different doses did not significantly affect the digestibility of nutrients, blood serum biochemical constituent, inner body organs, and markers of functions of liver and renal organs. In conclusion, the highest BWG and the best FCR and EPI for the whole period were from broilers given continuous 1 and 1.5 mL/L drinking water or intermittent multienzyme supplementation at 1.5 mL/L drinking water. Furthermore, intermittent supplementation of enzymes at 1 mL/L drinking water and continuous supplementation at 0.5 mL/L drinking water can be investigated in further experiments as a tool to improve broiler growth performance and economic traits and to decrease the cost of enzyme application.

scholarly journals Regulation of DNA (de)Methylation Positively Impacts Seed Germination during Seed Development under Heat Stress

Genes ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 457
Author(s):  
Jaiana Malabarba ◽  
David Windels ◽  
Wenjia Xu ◽  
Jerome Verdier
Keyword(s):  
Gene Expression ◽  
Heat Stress ◽  
Seed Germination ◽  
Seed Development ◽  
Molecular Mechanisms ◽  
Dna Demethylation ◽  
Mild Stress ◽  
Germination Capacity ◽  
Heat Stress Response ◽  
The Impact

Seed development needs the coordination of multiple molecular mechanisms to promote correct tissue development, seed filling, and the acquisition of germination capacity, desiccation tolerance, longevity, and dormancy. Heat stress can negatively impact these processes and upon the increase of global mean temperatures, global food security is threatened. Here, we explored the impact of heat stress on seed physiology, morphology, gene expression, and methylation on three stages of seed development. Notably, Arabidopsis Col-0 plants under heat stress presented a decrease in germination capacity as well as a decrease in longevity. We observed that upon mild stress, gene expression and DNA methylation were moderately affected. Nevertheless, upon severe heat stress during seed development, gene expression was intensively modified, promoting heat stress response mechanisms including the activation of the ABA pathway. By analyzing candidate epigenetic markers using the mutants’ physiological assays, we observed that the lack of DNA demethylation by the ROS1 gene impaired seed germination by affecting germination-related gene expression. On the other hand, we also observed that upon severe stress, a large proportion of differentially methylated regions (DMRs) were located in the promoters and gene sequences of germination-related genes. To conclude, our results indicate that DNA (de)methylation could be a key regulatory process to ensure proper seed germination of seeds produced under heat stress.

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