scholarly journals Effect of Seed Priming with Potassium Nitrate on the Performance of Tomato

Agriculture ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 498
Author(s):  
Muhammad Moaaz Ali ◽  
Talha Javed ◽  
Rosario Paolo Mauro ◽  
Rubab Shabbir ◽  
Irfan Afzal ◽  
...  
Keyword(s):  
Crop Production ◽  
Soluble Sugars ◽  
Seed Priming ◽  
Potassium Nitrate ◽  
Poor Quality ◽  
Growth Chamber ◽  
Tomato Seed ◽  
Tomato Seeds ◽  
Plant Growth Promoting ◽  
Greenhouse Screening

The seed industry and farmers have challenges, which include the production of poor quality and non-certified tomato seed, which ultimately results in decreased crop production. The issue carefully demands pre-sowing treatments using exogenous chemical plant growth-promoting substances. Therefore, to mitigate the above-stated problem, a series of experiments were conducted to improve the quality of tomato seeds (two cultivars, i.e., “Sundar” and “Ahmar”) and to enhance the stand establishment, vigor, physiological, and biochemical attributes under growth chamber and greenhouse conditions by using potassium nitrate (KNO3) as a seed priming agent. Seeds were imbibed in 0.25, 0.50, 0.75, 1.0, and 1.25 KNO3 (weight/volume) for 24 h and then dried before experiments. The results of growth chamber and greenhouse screening show that experimental units receiving tomato seeds primed with 0.75% KNO3 in both cultivars performed better as compared to other concentrations and nonprimed control. Significant increase in final emergence (%), mean emergence time, and physiological attributes were observed with 0.75% KNO3. Collectively, the improved performance of tomato due to seed priming with 0.75% KNO3 was linked with higher activities of total soluble sugars and phenolics under growth chamber and greenhouse screening.

scholarly journals Seed Priming With an Animal-derived Protein Hydrolysate Improves Drought Tolerance of Tomato Seeds by Enhancing Reserve Mobilization, Osmotic Adjustment, and Antioxidant Mechanism

2021 ◽  
Author(s):  
Weixuan Wang ◽  
Chenglong Zhang ◽  
Wenlong Zheng ◽  
Haofeng Lv ◽  
Weiwei Zhou ◽  
...  
Keyword(s):  
Drought Stress ◽  
Seed Germination ◽  
Seedling Growth ◽  
Soluble Sugar ◽  
Seed Priming ◽  
Protein Hydrolysates ◽  
Tomato Seed ◽  
Reserve Mobilization ◽  
Tomato Seeds ◽  
Germination Traits

Abstract Purpose Protein hydrolysates obtained from agro-industrial byproducts have received much attentions due to their positive roles in regulating plant responses to environmental stresses. However, little is known about the roles of animal protein hydrolysates in mediating seed drought tolerance and the underlying mechanism. Here, the effects of seed priming with pig blood protein hydrolysates (PP) on tomato seed germination and seedling growth under drought stress were investigated. Methods Tomato seeds were soaked with different concentrations of PP solutions for 24 h, and then transferred to filter paper moistened with distilled water or 10% PEG-6000 solution in Petri dish. The germination traits, seeding growth, reserve mobilization, osmolytes, and antioxidant system were determined.Results PP priming effectively alleviated the reduction in seed germination traits, resulting in improved tomato seedling growth under drought stress. PP priming enhanced amylase and sucrose synthase activities, soluble sugar, soluble protein, and free amino acid levels, thereby promoting reserve mobilization in seeds. Moreover, PP priming also reduces osmotic toxicity by increasing the accumulation of proline, soluble protein, and soluble sugar. Drought stress substantially enhanced the production of ROS and subsequent increases in MDA and Evans blue uptake, which were significantly alleviated after PP priming by improving the activities of SOD, POD, and CAT, and non-enzymatic antioxidants. Conclusion PP priming is a feasible method for improving tomato seed germination and seedling growth under drought stress by enhancing reserve mobilization, osmolyte accumulation, and antioxidant systems.

Comparative Advantage of Using Biopesticides in Ukrainian Agroecosystems

2020 ◽  
Vol 2 (6) ◽  
Author(s):  
Hasrat Arjjumend ◽  
Konstantia Koutouki ◽  
Olga Donets
Keyword(s):  
Shelf Life ◽  
Crop Production ◽  
Plant Protection ◽  
Agricultural Practices ◽  
Crop Productivity ◽  
Poor Quality ◽  
Soil Biology ◽  
Chemical Contamination ◽  
High Concentration ◽  
Irreversible Damage

The use of unsustainable levels of chemical fertilizers and plant protection chemicals has resulted in a steady decline in soil and crop productivity the world over. Soil biology has undergone irreversible damage, coupled with a high concentration of toxic chemical residues in plant tissues and human bodies. Agricultural practices must evolve to sustainably meet the growing global demand for food without irreversibly damaging soil. Microbial biocontrol agents have tremendous potential to bring sustainability to agriculture in a way that is safe for the environment. Biopesticides do not kill non-target insects, and biosafety is ensured because biopesticides act as antidotes and do not lead to chemical contamination in the soil. This article is part of a larger study conducted in Ukraine by researchers at the Université de Montréal with the support of Mitacs and Earth Alive Clean Technologies. The responses of farmers who use biofertilizers (“user farmers”) and those who do not (“non-user farmers”), along with the responses of manufacturers or suppliers of biofertilizers, and research and development (R&D) scientists are captured to demonstrate the advantages of applying microbial biopesticides to field crops. Participants reported a 15-30% increase in yields and crop production after the application of biopesticides. With the use of biopesticides, farmers cultivated better quality fruits, grains, and tubers with a longer shelf life. Moreover, while the risk of crop loss remains high (60-70%) with chemically grown crops, this risk is reduced to 33% on average if crops are grown using biopesticides. The findings indicate that a large proportion of farmers would prefer to use biopesticides if they are effective and high quality products. In this context, the quality and effectiveness of products is therefore very important. Despite their benefits to soil, human health, and ecosystems, biopesticides face significant challenges and competition vis-à-vis synthetic pesticides for a variety of reasons. Therefore, the development of biopesticides must overcome the problems of poor quality products, short shelf life, delayed action, high market costs, and legal/registration issues.

scholarly journals Scaling-up production of plant endophytes in bioreactors: concepts, challenges and perspectives

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Seedhabadee Ganeshan ◽  
Seon Hwa Kim ◽  
Vladimir Vujanovic
Keyword(s):  
Crop Production ◽  
Process Development ◽  
Hazard Analysis ◽  
Scale Up ◽  
Process Technology ◽  
Lack Of Information ◽  
Agriculture Industry ◽  
Start Up ◽  
Plant Growth Promoting ◽  
Agriculture And Food

AbstractThe benefit of microorganisms to humans, animals, insects and plants is increasingly recognized, with intensified microbial endophytes research indicative of this realization. In the agriculture industry, the benefits are tremendous to move towards sustainable crop production and minimize or circumvent the use of chemical fertilizers and pesticides. The research leading to the identification of potential plant endophytes is long and arduous and for many researchers the challenge is ultimately in scale-up production. While many of the larger agriculture and food industries have their own scale-up and manufacturing facilities, for many in academia and start-up companies the next steps towards production have been a stumbling block due to lack of information and understanding of the processes involved in scale-up fermentation. This review provides an overview of the fermentation process from shake flask cultures to scale-up and the manufacturing steps involved such as process development optimization (PDO), process hazard analysis (PHA), pre-, in- and post-production (PIP) challenges and finally the preparation of a technology transfer package (TTP) to transition the PDO to manufacturing. The focus is on submerged liquid fermentation (SLF) and plant endophytes production by providing original examples of fungal and bacterial endophytes, plant growth promoting Penicillium sp. and Streptomyces sp. bioinoculants, respectively. We also discuss the concepts, challenges and future perspectives of the scale-up microbial endophyte process technology based on the industrial and biosafety research platform for advancing a massive production of next-generation biologicals in bioreactors.

scholarly journals Efficacy of KNO3, SiO2 and SA priming for improving emergence, seedling growth and antioxidant enzymes of rice (Oryza sativa), under drought

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Lawan Gana Ali ◽  
Rosimah Nulit ◽  
Mohd Hafiz Ibrahim ◽  
Christina Yong Seok Yien
Keyword(s):  
Salicylic Acid ◽  
Seedling Growth ◽  
Antioxidant Activities ◽  
Soluble Sugar ◽  
Seed Priming ◽  
Potassium Nitrate ◽  
Total Soluble Protein ◽  
Rice Seedlings ◽  
Chlorophyll Contents ◽  
Randomized Design

AbstractRice is an important staple crop produced and consumed worldwide. However, poor seed emergence is one of the main impediments to obtaining higher yield of rice especially in hot and dry ecosystems of the world that are ravaged by drought. Therefore, this study was carried out to evaluate the effects of potassium nitrate (KNO3), salicylic acid (SA) and silicon dioxide (SiO2) priming in improving emergence, seedling growth, biochemical attributes and antioxidant activities of FARO44 rice under drought conditions. Rice seedlings primed with 2.5% and 5% KNO3, 3% and 3.5% SiO2, and 1 mM and 2.5 mM SA were subjected to three drought levels of low, moderate and severe under the greenhouse. Seed emergence, seedling growth, biochemical attributes and antioxidant activities were thereafter evaluated. Seed priming experiments were laid in a completely randomized design with five replicates per treatment. The results found that rice seedlings responded differently to different priming treatments. However, all primed rice seedlings had significantly (P ≤ 0.05) improved emergence percentage (72–92%), seedling growth, seedling vigor, seedling fresh and dry biomass and shorter emergence time compared with controls. Likewise, total soluble protein content, activities of catalase, ascorbate peroxidase and superoxide dismutase, carbohydrate, soluble sugar and total chlorophyll contents of rice seedlings were increased by more than two-folds by seed priming compared with control. Salicylic acid showed less effect in increasing emergence, seedling growth, antioxidant activities and biochemical attributes of rice. Thus, this study established that seed priming with KNO3 (2.5% and 5%) and SiO2 (3% and 3.5%) were more effective in improving emergence, seedling growth, biochemical attributes and antioxidant activities of FARO44. Thus, priming of FARO44 rice with this chemical is recommended for fast emergence, seedling growth and drought resistance in dry ecosystems.

scholarly journals Seed Priming with Potassium Nitrate and Gibberellic Acid Enhances the Performance of Dry Direct Seeded Rice (Oryza sativa L.) in North-Western India

Agronomy ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 849
Author(s):  
Buta Singh Dhillon ◽  
Virender Kumar ◽  
Pardeep Sagwal ◽  
Navjyot Kaur ◽  
Gurjit Singh Mangat ◽  
...  
Keyword(s):  
Gibberellic Acid ◽  
Grain Yield ◽  
Oryza Sativa L ◽  
Seed Priming ◽  
Potassium Nitrate ◽  
Growth And Yield ◽  
Western India ◽  
Yield Attributes ◽  
Crop Establishment ◽  
Direct Seeded

Poor early growth and uneven crop establishment are reported as the major bottlenecks in wide-scale adoption and optimal yield realization of dry direct-seeded rice (DSR). Seed priming can potentially help overcome these problems in DSR. Therefore, laboratory and field studies were conducted at Punjab Agricultural University, Ludhiana, India, during kharif/wet-season 2018 and 2019 to evaluate the effect of different priming techniques on germination, establishment, growth, and grain yield of rice under DSR conditions. The following priming treatments were evaluated: dry non-primed seed (control), hydropriming with distilled water, halopriming with 2.0% potassium nitrate, hormopriming with 50 ppm gibberellic acid (GA3), and osmopriming with polyethylene glycol (PEG)(−0.6 MPa), each with 12 and 24 h priming duration. In 2019, priming treatments were tested under two DSR establishment methods—conventional DSR (sowing in dry soil followed by irrigation) and soil mulch DSR (locally known as vattar DSR) (sowing in moist soil after pre-sowing irrigation), whereas in 2018, priming treatments were evaluated under conventional DSR only. In both years, halopriming and hormopriming resulted in a 7–11% increase in rice yields compared to non-primed dry seed (control). Osmopriming resulted in a 4% yield increase compared to control in 2018 but not in 2019. The higher yields in halopriming and hormopriming were attributed to higher and rapid germination/crop emergence, better root growth, and improvement in yield attributes. Priming effect on crop emergence, growth, and yield did not differ by DSR establishment methods and duration of priming. Conventional DSR and soil mulch DSR did not differ in grain yield, whereas they differed in crop emergence, growth, and yield attributes. These results suggest that halopriming with 2.0% potassium nitrate and hormopriming with 50 ppm GA3 has good potential to improve crop establishment and yield of rice in both conventional and soil mulch DSR systems.

scholarly journals Effect of Spaceflight on Tomato Seed Quality and Biochemical Characteristics of Mature Plants

Horticulturae ◽  
2021 ◽  
Vol 7 (5) ◽  
pp. 89
Author(s):  
Elena Dzhos ◽  
Nadezhda Golubkina ◽  
Marina Antoshkina ◽  
Irina Kondratyeva ◽  
Andrew Koshevarov ◽  
...  
Keyword(s):  
Seed Quality ◽  
Space Station ◽  
Field Conditions ◽  
Tomato Seed ◽  
Tomato Plants ◽  
Biochemical Characteristics ◽  
Tomato Seeds ◽  
Genetic Modifications ◽  
Total Antioxidant

Intensive space exploration includes profound investigations on the effect of weightlessness and cosmic radiation on plant growth and development. Tomato seeds are often used in such experiments though up to date the results have given rather vague information about biochemical changes in mature plants grown from seeds subjected to spaceflight. The effect of half a year of storage in the International Space Station (ISS) on tomato seeds (cultivar Podmoskovny ranny) was studied by analyzing the biochemical characteristics and mineral content of mature plants grown from these seeds both in greenhouse and field conditions. A significant increase was recorded in ascorbic acid, polyphenol and carotenoid contents, and total antioxidant activity (AOA), with higher changes in the field conditions compared to greenhouse. Contrary to control plants, the ones derived from space-stored seeds demonstrated a significant decrease in root AOA. The latter plants also showed a higher yield, but lower content of fruit dry matter, sugars, total dissolved solids and organic acids. The fruits of plants derived from space-stored seeds demonstrated decreased levels of Fe, Cu and taste index. The described results reflect the existence of oxidative stress in mature tomato plants as a long-term consequence of the effect of spaceflight on seed quality, whereas the higher yield may be attributed to genetic modifications.

Interactive effect of biochar and plant growth-promoting bacterial endophytes on ameliorating salinity stress in maize

2015 ◽  
Vol 42 (8) ◽  
pp. 770 ◽  
Author(s):  
Saqib Saleem Akhtar ◽  
Mathias Neumann Andersen ◽  
Muhammad Naveed ◽  
Zahir Ahmad Zahir ◽  
Fulai Liu
Keyword(s):  
Plant Growth ◽  
Salinity Stress ◽  
Crop Production ◽  
Interactive Effect ◽  
Nutrient Balance ◽  
Bacterial Strains ◽  
Negative Effects ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Salt Affected Soils

The objective of this work was to study the interactive effect of biochar and plant growth-promoting endophytic bacteria containing 1-aminocyclopropane-1-carboxylate deaminase and exopolysaccharide activity on mitigating salinity stress in maize (Zea mays L.). The plants were grown in a greenhouse under controlled conditions, and were subjected to separate or combined treatments of biochar (0% and 5%, w/w) and two endophytic bacterial strains (Burkholderia phytofirmans (PsJN) and Enterobacter sp. (FD17)) and salinity stress. The results indicated that salinity significantly decreased the growth of maize, whereas both biochar and inoculation mitigated the negative effects of salinity on maize performance either by decreasing the xylem Na+ concentration ([Na+]xylem) uptake or by maintaining nutrient balance within the plant, especially when the two treatments were applied in combination. Moreover, in biochar-amended saline soil, strain FD17 performed significantly better than did PsJN in reducing [Na+]xylem. Our results suggested that inoculation of plants with endophytic baterial strains along with biochar amendment could be an effective approach for sustaining crop production in salt-affected soils.

The potential of the smoke-derived compound 3-methyl-2H-furo[2,3-c]pyran-2-one as a priming agent for tomato seeds

2007 ◽  
Vol 17 (3) ◽  
pp. 175-181 ◽  
Author(s):  
Neeru Jain ◽  
Johannes Van Staden
Keyword(s):  
Large Scale ◽  
Seed Storage ◽  
Seed Priming ◽  
Stress Conditions ◽  
Scale Production ◽  
Seedling Vigour ◽  
Flow Cytometry Data ◽  
Tomato Seeds ◽  
Vigour Index ◽  
Continuous Presence

AbstractThe stimulatory role of 3-methyl-2H-furo[2,3-c]pyran-2-one, a smoke-derived butenolide, on germination and post-germination events is well documented. Previous studies have involved germinating seeds in the continuous presence of the compound. However, commercial growers cannot exploit the potential of the butenolide for large-scale production of crops due to limited availability and environmental constraints. The present study was undertaken to assess the potential of the butenolide as a priming agent of tomato (Solanum esculentum Mill.) seeds. Flow cytometry data revealed that butenolide-primed seeds had a higher percentage of nuclei at the 4C stage than water-primed seeds. Emergence of the radicle was much faster in the primed seeds. After 36 h of imbibition, a higher percentage of the butenolide-primed seeds (22%) exhibited radicle emergence compared to the water-primed seeds (12%). While butenolide-primed seeds initially germinated more rapidly than either water-primed or unprimed seeds in a 48-h period, water-imbibed seeds reached a similar germination level as the butenolide-primed seeds by 60 h of incubation. The butenolide-primed seeds produced significantly (P ≤ 0.05) more vigorous seedlings than water-primed seeds or seeds in the continuous presence of either water or butenolide. A gradual decrease in the seedling vigour index was recorded for both water and butenolide-primed seeds with increased seed storage at room temperature. Nevertheless, the vigour index was significantly greater in the butenolide-primed seeds than the water-primed seeds. Vigour indices were significantly (P ≤ 0.05) higher for the butenolide-primed seeds under various stress conditions (salinity, osmoticum or temperature) compared to control or water-primed seeds. Results of the present study suggest that the butenolide is a good seed-priming agent. Additionally, primed seeds retained the promotive effect for a considerable time. This was also the case for tomato seeds under various simulated field stress conditions.

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