Dissecting the Gene Expression Networks Associated with Variations in the Major Components of the Fatty Acid Semimembranosus Muscle Profile in Large White Heavy Pigs

To date, high-throughput technology such as RNA-sequencing has been successfully applied in livestock sciences to investigate molecular networks involved in complex traits, such as meat quality. Pork quality depends on several organoleptic, technological, and nutritional characteristics, and it is also influenced by the fatty acid (FA) composition of intramuscular fat (IMF). To explore the molecular networks associated with different IMF FA compositions, the Semimembranosus muscle (SM) from two groups of Italian Large White (ILW) heavy pigs divergent for SM IMF content was investigated using transcriptome analysis. After alignment and normalization, the obtained gene counts were used to perform the Weighted Correlation Network Analysis (WGCNA package in R environment). Palmitic and palmitoleic contents showed association with the same gene modules, comprising genes significantly enriched in autophagy, mitochondrial fusion, and mitochondrial activity. Among the key genes related to these FAs, we found TEAD4, a gene regulating mitochondrial activity that seems to be a promising candidate for further studies. On the other hand, the genes comprised in the modules associated with the IMF contents of oleic, n-6, and n-3 polyunsaturated FAs (PUFAs) were significantly enriched in Mitogen-Activated Protein Kinase (MAPK) signaling, in agreement with previous studies suggesting that several MAPK players may have a primary role in regulating lipid deposition. These results give an insight into the molecular cascade associated with different IMF FA composition in ILW heavy pigs. Further studies are needed to validate the results and confirm whether some of the identified key genes may be effective candidates for pork quality.

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Impact of an Infant Formula Containing a Novel Fat Blend (Cow’s Milk Fat, Fish and Vegetable Oil) and Prebiotics on Stool Fatty Acid Soaps and Erythrocyte Fatty Acid Profiles in Full-Term Healthy Newborns

Annals of Nutrition and Metabolism ◽

10.1159/000515705 ◽

2021 ◽

pp. 1-8

Author(s):

Maroula Lambidou ◽

Birgit Alteheld ◽

Rolf Fimmers ◽

Frank Jochum ◽

Antonia Nomayo ◽

...

Keyword(s):

Body Weight ◽

Fatty Acid ◽

Infant Formula ◽

Milk Fat ◽

Control Group ◽

Human Breast Milk ◽

Infant Formulas ◽

Fa Composition ◽

And Control ◽

Breast Fed

Introduction: Recently, new commercial infant formulas have been composed considering novel fat blends and oligosaccharides to better resemble the fatty acid (FA) composition and stereospecific distribution (e.g., increased amount of ß-palmitate) as well as probiotics content of human breast milk. We hypothesized that these newly composed infant formulas may decrease fecal FA soap excretion and may positively affect erythrocyte FA profiles compared with regular formulas. Methods: Healthy infants were randomly assigned to receive a high-sn-2-palmitate formula (>25% of the PA is esterified to the sn-2 position of the glycerol backbone, verum: n = 30) or a “standard” formula containing <10% of PA in sn-2 position and no oligosaccharides (control: n = 27); a non-randomized group of breast-fed infants served as control. Anthropometric data of the infants (body weight, recumbent length, and head circumference) were recorded at inclusion (visit 1) and 6 and 12 weeks after onset of intervention (visits 2 and 3). Blood samples for erythrocyte FA analysis (gas chromatography) were taken at visits 1 and 2; stool samples were collected at visit 2. Results: Quantitative formula intake (mL/kg body weight × day) at visit 2 (verum: 155 ± 30, control: 164 ± 30) and visit 3 (verum: 134 ± 26, control: 134 ± 21) was comparable. Six weeks after onset of intervention, stool total FA soaps, palmitate soaps, and total FAs were similar in both formula-fed groups but significantly higher than in breast-fed infants. During the 6-week intervention, erythrocyte palmitate decreased significantly from baseline in all 3 groups with no group differences (verum: 29.20 ± 1.17 to 27.12 ± 0.66, control: 29.88 ± 2.00 to 27.01 ± 0.94, breast-fed: 30.20 ± 0.86 to 26.84 ± 0.98). For selected FAs, significant changes over time in verum and control group were obvious but without formula effects. Some variations in the FA profile of breast-fed infants compared to both verum and control groups were observed. Conclusions: In contrast to our hypothesis, feeding a newly composed infant formula based on a fat blend with 25% of PA in the sn-2 position of triacylglycerols and supplemented with a prebiotic could not decrease insoluble FA soap excretion compared with a standard product; in this respect, breastfeeding is obviously the best choice. Surprisingly, erythrocyte FA profiles were comparable in formula-fed and breast-fed infants; obvious alterations in FA composition of the respective fat sources and structure did not affect FA incorporation into membranes. Caution should be, however, exercised in drawing robust conclusions in the absence of larger, adequately powered intervention studies.

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PSVII-18 Fatty acid composition of the longissimus muscle from Angus and Wagyu sired cattle at the 6th and 12th rib location

Journal of Animal Science ◽

10.1093/jas/skaa054.366 ◽

2020 ◽

Vol 98 (Supplement_3) ◽

pp. 211-212

Author(s):

Jerad Jaborek ◽

Francis L Fluharty ◽

Alejandro E Relling

Keyword(s):

Fatty Acid ◽

Mixed Model ◽

Random Effect ◽

Repeated Measurements ◽

Longissimus Muscle ◽

Trans Isomers ◽

Fa Composition ◽

Effect Of Treatment ◽

Similar Body ◽

Location Interaction

Abstract The fatty acid (FA) composition of the longissimus muscle (LM) of Angus and Wagyu sired cattle raised to a similar body weight (612 kg) were compared at the 6th and 12th rib locations. Angus sired steers represented T1, cattle from a Wagyu sire selected for growth represented T2, and cattle from a Wagyu sire selected for marbling represented T3. Data were analyzed mixed model with repeated measurements on animal (LM location); the model include the fixed effect of treatment, LM location, and their interaction, and random effect of sex. The percentage of 16:0, 18:1cis9, 18:3, and monounsaturated FA (MUFA) exhibited a treatment*LM location interaction (P ≤ 0.7), where T2 cattle had a greater percentage of 16:0 and a lesser percentage of 18:1cis9, 18:3, and MUFA at the 12th rib vs. 6th rib location compared with T1 and T3 cattle. The percentage of total FA lipid, polyunsaturated FA(PUFA), and PUFA:SFA ratio in the LM were greater (P ≤ 0.02) for T3 cattle compared with T1 and T2 cattle. The percentage of 18:0 was greater (P ≤ 0.01) for T1 cattle compared with T2 and T3 cattle, while T1 cattle had a greater (P ≤ 0.01) percentage of saturated FA (SFA) compared to T3 cattle. The percentage of 18:1cis9, other 18:1cis isomers, 18:2, MUFA, and MUFA:SFA ratio were greater (P ≤ 0.02) for T3 cattle compared with T1 cattle, with T2 cattle being intermediate. The percentage of total FA lipid, 18:0, 18:1 trans isomers, and SFA were greater (P ≤ 0.01) at the 6th rib LM location, while 14:1, 18:cis9, other 18:1 cis isomers, MUFA, MUFA:SFA, and PUFA:SFA ratio were greater (P ≤ 0.02) at the 12th rib LM location

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Effects of full-time v. part-time grazing on seasonal changes in milk coagulation properties and fatty acid composition

Journal of Dairy Research ◽

10.1017/s0022029921000169 ◽

2021 ◽

pp. 1-6

Author(s):

Franziska S. Akert ◽

Michael Kreuzer ◽

Carmen Kunz ◽

Beat Reidy ◽

Joel Berard

Keyword(s):

Fatty Acid ◽

Seasonal Variations ◽

Milk Fat ◽

Seasonal Effects ◽

Full Time ◽

Fa Composition ◽

Part Time ◽

Milk Coagulation ◽

The Impact ◽

Feeding Systems

Abstract For this research communication our objective was to investigate to what extent milk coagulation properties and milk fatty acid (FA) composition were affected by different feeding systems, season and their interaction. Eighteen cows in total were subjected to one of three different feeding system treatments: full-time grazing or part-time grazing combined with indoor feeding of fresh grass with low or high concentrate supplementation. Milk was sampled in spring, summer and autumn. Milk coagulation time was 15.0, 19.0 and 17.7 min, coagulation dynamics 1.67, 3.41 and 1.79 min, and curd firmness 52.7, 32.4 and 47.0 mm in spring, summer and autumn, respectively. Thus, milk coagulation properties of the milk were lower during summer. There were strong seasonal effects on milk FA proportions, but there were not always changes with progressing season, or changes were different with respect to the impact of the feeding systems (system × season interaction). The milk fat was favourably rich in oleic acid, conjugated linoleic acid and α-linolenic acid and had a low n-6/n-3 fatty acid ratio in all systems. Factors like seasonal variations in grass composition and the energy balance of the cows were considered relevant for the milk FA composition. Overall, seasonal variations in milk quality were less pronounced with part-time grazing with fresh grass indoors as compared to full-time grazing without concentrate.

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Effect of almond consumption on the serum fatty acid profile: a dose–response study

British Journal Of Nutrition ◽

10.1017/s0007114514001640 ◽

2014 ◽

Vol 112 (7) ◽

pp. 1137-1146 ◽

Cited By ~ 19

Author(s):

Stephanie Nishi ◽

Cyril W. C. Kendall ◽

Ana-Maria Gascoyne ◽

Richard P. Bazinet ◽

Balachandran Bashyam ◽

...

Keyword(s):

Fatty Acid ◽

Risk Score ◽

Percentage Change ◽

Control Group ◽

Dependent Manner ◽

Serum Fatty Acid ◽

Full Dose ◽

Fa Composition ◽

Chd Risk ◽

Half Dose

Consumption of almonds has been shown to be associated with a decreased risk of CHD, which may be related to their fatty acid (FA) composition. However, the effect of almond consumption on the serum FA composition is not known. Therefore, in the present study, we investigated whether almond consumption would alter the serum FA profile and risk of CHD, as calculated using Framingham's 10-year risk score, in a dose-dependent manner in hyperlipidaemic individuals when compared with a higher-carbohydrate control group using dietary interventions incorporating almonds. A total of twenty-seven hyperlipidaemic individuals consumed three isoenergetic (mean 1770 kJ/d) supplements during three 1-month dietary phases: (1) full-dose almonds (50–100 g/d); (2) half-dose almonds with half-dose muffins; (3) full-dose muffins. Fasting blood samples were obtained at weeks 0 and 4 for the determination of FA concentrations. Almond intake (g/d) was found to be inversely associated with the estimated Framingham 10-year CHD risk score (P= 0·026). In both the half-dose and full-dose almond groups, the proportions of oleic acid (OA) and MUFA in the TAG fraction (half-almond: OA P= 0·003; MUFA P= 0·004; full-almond: OA P< 0·001; MUFA P< 0·001) and in the NEFA fraction (half-almond: OA P= 0·01; MUFA P= 0·04; full-almond: OA P= 0·12; MUFA P= 0·06) increased. The estimated Framingham 10-year CHD risk score was inversely associated with the percentage change of OA (P= 0·011) and MUFA (P= 0·016) content in the TAG fraction. The proportions of MUFA in the TAG and NEFA fractions were positively associated with changes in HDL-cholesterol concentrations. Similarly, the estimated Framingham 10-year CHD risk score was inversely associated with the percentage change of OA (P= 0·069) and MUFA content in the NEFA fraction (P= 0·009). In conclusion, the results of the present study indicate that almond consumption increases OA and MUFA content in serum TAG and NEFA fractions, which are inversely associated with CHD lipid risk factors and overall estimated 10-year CHD risk.

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Fat mobilization in Pietrain and Large White pigs

British Journal Of Nutrition ◽

10.1079/bjn19770019 ◽

1977 ◽

Vol 37 (2) ◽

pp. 167-186 ◽

Cited By ~ 43

Author(s):

J. D. Wood ◽

N. G. Gregory ◽

G. M. Hall ◽

D. Lister

Keyword(s):

Body Weight ◽

Oleic Acid ◽

Fatty Acid ◽

Subcutaneous Fat ◽

Entry Rate ◽

Large White ◽

Insulin Metabolism ◽

Fat Mobilization ◽

Body Tissues ◽

Norepinephrine Infusion

1. Two experiments were done with Pietrain and Large White pigs (about 50 kg body-weight) to determine whether fat mobilization is enhanced in the stress-sensitive Pietrains (which also produce pale, soft, exudative (PSE) meat).2. In Expt 1, plasma glucose and insulin concentrations and free fatty acid (FFA) concentrations and composition were measured in five Pietrains and five Large Whites after feeding, during an infusion of norepinephrine (2.5 μg/kg body-weight per min), 16 and 21 h after the withdrawal of food and following insulin administration (0.3 IU/kg body-weight). The entry rate of oleic acid was measured 4 h after feeding. Body composition and longissimus dorsi pH measurements were made on killing the pigs 4 d after the experiment.3. After feeding, the Pietrains tended to have lower concentrations of glucose and insulin in plasma compared with the Large Whites, and higher concentrations of FFA. The Pietrains also had a faster entry rate of oleic acid into body tissues. In fasting (16 h) these differences in hormone and metabolite concentrations were also present and relationships between them and body development could be detected. Pietrains had higher concentrations of FFA and lower concentrations of insulin than Large Whites at a particular stage of the development of fat and muscle (subcutaneous fat weight÷longissimus muscle weight).4. The fatty acid composition of FFA resembled that of the backfat triglycerides in fasting and during norepinephrine infusion but not in the fed state. In particular the proportion of fatty acid 18:1 was low in the fed pigs and that of 18:2 was high. The contrast in FFA composition between the fed and stimulated state was greater in the Large Whites.5. The Pietrains were less sensitive to the antilipolytic action of insulin. Glucose and FFA concentrations were similar in both breeds during the norepinephrine infusion although insulin concentrations were increased to a larger extent in the Large Whites at the termination of the infusion.6. In Expt 2, anaesthetized Pietrain and Large White pigs were given constant infusions of norepinephrine alone (2.5 μg/kg body-weight per min) and norepinephrine+propranolol (2 and 10 μg/kg body-weight per min) or phentolamine (2 and 10 μg/kg body-weight per min). The aim was to determine whether the breeds differed in their sensitivity to norepinephrine when conscious responses to the hormone were prevented.7. When norepinephrine alone was infused, fat mobilization was greater in the Pietrain pigs and glucose concentrations were greater in the Large Whites. Propranolol markedly reduced the lipolytic action of norepinephrine, particularly in the Pietrain pigs studied, and phentolamine reduced its glycogenolytic action, particularly in the Large White pig.8. It seems that the leanness of stress-sensitive, PSE-susceptible Pietrain pigs may be due to an enhanced fat mobilization under various conditions, associated with an impairment in insulin metabolism and a greater sensitivity to the β-adrenergic action of catecholamines on body fat stores.

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Deletion of nuclear factor-κB p50 upregulates fatty acid utilization and contributes to an anti-obesity and high-endurance phenotype in mice

AJP Endocrinology and Metabolism ◽

10.1152/ajpendo.00071.2015 ◽

2015 ◽

Vol 309 (6) ◽

pp. E523-E533 ◽

Cited By ~ 5

Author(s):

Yoshihiko Minegishi ◽

Satoshi Haramizu ◽

Koichi Misawa ◽

Akira Shimotoyodome ◽

Tadashi Hase ◽

...

Keyword(s):

Fatty Acid ◽

Nuclear Factor Κb ◽

Whole Body ◽

Acid Oxidation ◽

Endurance Capacity ◽

Peroxisome Proliferator ◽

Primary Role ◽

Nuclear Factor ◽

Oxidation Activity ◽

Fatty Acid Utilization

The transcription factor nuclear factor-κB (NF-κB) plays an important role in regulating physiological processes such as immunity and inflammation. In addition to this primary role, NF-κB interacts physically with peroxisome proliferator-activated receptors regulating lipid metabolism-related gene expression and inhibits their transcriptional activity. Therefore, inhibition of NF-κB may promote fatty acid utilization, which could ameliorate obesity and improve endurance capacity. To test this hypothesis, we attempted to elucidate the energy metabolic status of mice lacking the p50 subunit of NF-κB (p50 KO mice) from the tissue to whole body level. p50 KO mice showed a significantly lower respiratory quotient throughout the day than did wild-type (WT) mice; this decrease was associated with increased fatty acid oxidation activity in liver and gastrocnemius muscle of p50 KO mice. p50 KO mice that were fed a high-fat diet were also resistant to fat accumulation and adipose tissue inflammation. Furthermore, p50 KO mice showed a significantly longer maximum running time compared with WT mice, with a lower respiratory exchange ratio during exercise as well as higher residual muscle glycogen content and lower blood lactate levels after exercise. These results suggest that p50 deletion facilitates fatty acid catabolism, leading to an anti-obesity and high-endurance phenotype of mice and supporting the idea that NF-κB is an important regulator of energy metabolism.

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Effects of finishing diet and pre-slaughter fasting time on meat quality in crossbred pigs

Agricultural and Food Science ◽

10.2137/145960607783328182 ◽

2008 ◽

Vol 16 (3) ◽

pp. 245 ◽

Cited By ~ 7

Author(s):

K. PARTANEN ◽

H. SILJANDER-RASI ◽

M. HONKAVAARA

Keyword(s):

Control Diet ◽

Eating Quality ◽

Pork Quality ◽

Carbohydrate Composition ◽

Finishing Diets ◽

Semimembranosus Muscle ◽

Fasting Time ◽

Longissimus Lumborum ◽

Fibrous Diet ◽

Crossbred Pigs

The effects of the carbohydrate composition of finishing diet (fed from 80 to 107 kg of body weight) and the length of pre-slaughter fasting on pork quality were studied in a 2 × 2 factorial experiment with 80 crossbred pigs. The control finishing diet was based on barley and soybean meal, and the fibrous finishing diet was based on barley, barley fibre, faba beans, and rapeseed cake. These diets contained 465 and 362 g starch and 177 and 250 g dietary fibre per kg, respectively. The fasting times of 25 and 41 h were obtained by giving the pigs their last meal at different times. Longer fasting lowered the glycolytic potential of the longissimus lumborum muscle (P = 0.01), whereas the finishing diet had no effect. Different muscles responded differently to the treatments. Longer fasting increased the ultimate pH of the semimembranosus muscle (P = 0.02), but did not affect that of the longissimus lumborum and semispinalis capitis muscles. The finishing diets did not affect the ultimate pH of the investigated muscles. A diet × fasting time interaction was seen in the lightness of the semimembranosus muscle (P = 0.05). The fibrous diet resulted in darker meat than the control diet did in pigs that were fasted for 25 h (P < 0.05). Longer fasting darkened the meat colour in pigs fed the fibrous diet (P < 0.05) but not in those fed the control diet. The meat from the semispinalis capitis muscle was darker in pigs fed the fibrous than those fed the control diet (P = 0.04). The treatments did not affect the colour of the longissimus lumborum muscle. Longer fasting decreased drip loss from the meat of pigs fed the control diet (P < 0.05). The eating quality of the pork was not influenced by the finishing diets or the fasting time. The pigs also grew equally fast on both finishing diets. In conclusion, a moderate alteration in the carbohydrate composition of a finishing diet or longer pre-slaughter fasting can have some effects on pork quality in crossbred pigs, but these effects vary in different muscles.;

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Characterization of planktonic and biofilm fatty acid profiles and evaluation of their trophic transfer potential to Hyalella Azteca

10.32920/ryerson.14657085.v1 ◽

2021 ◽

Author(s):

Jerry Chien-Yao Chao

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Bacterial Species ◽

Saturated Fatty Acids ◽

Hyalella Azteca ◽

Fatty Acid Profiles ◽

Fa Composition ◽

Branched Chain ◽

Transfer Potential

Fatty acid (FA) composition between biofilms and batch planktonic cultures were compared for two bacterial species Pseudomonas aeruginosa and Staphylococcus aureaus. Biofilm cultures exhibited decrease in saturated fatty acids (SAFA) that potentially conform to a more fluidic biophysical membrane property. The amount of FA in the biofilms' extracellular polymeric substance was not sufficient to consider it having a major contribution to the observed differences between biofilms and batch planktonic cultures. While biofilm grazing by the amphipod Hyalella azteca was evident, only certain bacteria-specific FA appeared to have the potential to be retained (odd-number SAFA and branched-chain FA). H. azteca with diet strictly consisted of bacteria biofilms did not demonstrate significant changes in their nutritional condition in terms of ω-3 and ω-6 polyunsaturated fatty acids (PUFA): combined with the results from fasting trials, H. azteca appears to have the capacity to retain ω-3 and ω-6 PUFAs up to 10 days.

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Association of the heart fatty acid-binding protein gene with quality of carcass and meat traits in pigs

Arquivo Brasileiro de Medicina Veterinária e Zootecnia ◽

10.1590/s0102-09352008000200020 ◽

2008 ◽

Vol 60 (2) ◽

pp. 408-413 ◽

Cited By ~ 1

Author(s):

F.C. Figueiredo ◽

P.S. Lopes ◽

A.P.G. Pinto ◽

D.A.F. Paiva ◽

P.T. Mendonça ◽

...

Keyword(s):

Fatty Acid ◽

Fatty Acid Binding Protein ◽

Binding Protein ◽

Large White ◽

Fatty Acid Binding ◽

Acid Binding Protein ◽

Genetic Groups ◽

Pcr Products ◽

Fabp Gene

The heart fatty acid-binding protein (HFABP) gene was sequenced in parental animals of a F2 crossing of boars of the Brazilian native Piau breed with commercial sows (Landrace x Large White Pietrain). Primers used for PCR were designed to amplify four exons of the gene. The PCR products were sequenced and compared with the GenBank sequences. Differences between the generated sequences and the GenBank sequences were observed for both genetic groups. A total of 246 F2 animals were genotyped using the Hinf I restriction enzyme. Two genotypes were identified, 198 being animals HH and 48 Hh. The Hinf I SNP was significantly associated with weights of loin (bone-in) (P<0.05), jowl (P<0.05), sirloin (P<0.10), and kidneys (P<0.01). These results showed the potential of the H-FABP gene in marker-assisted selection programs for carcass traits in pigs.

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Comparative analysis of gene expression profiles in differentiated subcutaneous adipocytes between Jiaxing Black and Large White Pigs

10.21203/rs.3.rs-19837/v1 ◽

2020 ◽

Author(s):

Dawei Zhang ◽

Wenjing Wu ◽

Xin Huang ◽

Ke Xu ◽

Cheng Zheng ◽

...

Keyword(s):

Signaling Pathway ◽

Expression Profiles ◽

Mapk Signaling ◽

Fat Deposition ◽

Mapk Signaling Pathway ◽

Differentially Expressed ◽

Rna Seq ◽

Domestic Pig ◽

Large White ◽

Pig Breeds

Abstract Background: Chinese domestic pig breeds are reputed for pork quality, but their low ratio of lean-to-fat carcass weight decreases production efficiency. A better understanding of the genetic regulation network of SC fat tissue is necessary for the rational selection of Chinese domestic pig breeds. In the present study, SC adipocytes were isolated from Jiaxing Black pigs (a Chinese indigenous pig breed with redundant SC fat deposition) and Large White pigs (a lean-type pig breed with relatively low SC fat deposition) and the expression profiles of mRNAs and lncRNAs were compared by RNA-seq analysis to identify biomarkers correlated with the differences of SC fat deposition between the two breeds.Results: A total of 3,371 differentially expressed genes (DEGs) and 1,182 differentially expressed lncRNAs (DELs) were identified in SC adipocytes between Jiaxing Black (JX) and Large White (LW) pigs, which included 797 upregulated mRNAs, 2,574 downregulated mRNAs, 461 upregulated lncRNAs and 721 downregulated lncRNAs. Gene Ontology and KEGG pathway analyses revealed that the DEGs and DELs were mainly involved in the immune response, cell fate determination, PI3K-Akt signaling pathway and MAPK signaling pathway, which are known to be related to adipogenesis and lipid metabolism. The expression levels of DEGs and DELs according to the RNA-seq data were verified by quantitative PCR, which showed 81.8% consistency. The differences in MAPK pathway activity between JX and LW pigs was confirmed by western blot analysis, with <100-fold elevated p38 phosphorylation in JX pigs.Conclusions: This study offers a detailed characterization of mRNAs and lncRNAs in fat- and lean-type pig breeds. The activity of the MAPK signaling pathway was found to be associated with subcutaneous adipogenesis. These results greatly enhance our understanding of the molecular mechanisms regulating SC fat deposition in pigs.

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