scholarly journals Tailoring the Health-Promoting Potential of Protein Hydrolysate Derived from Fish Wastes and Flavonoids from Yellow Onion Skins: From Binding Mechanisms to Microencapsulated Functional Ingredients

Biomolecules ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1416
Author(s):  
Leontina Grigore-Gurgu ◽  
Oana Crăciunescu ◽  
Iuliana Aprodu ◽  
Carmen Alina Bolea ◽  
Andreea Iosăgeanu ◽  
...  
Keyword(s):  
Protein Hydrolysate ◽  
L929 Cell ◽  
Dry Weight ◽  
Biological Properties ◽  
Van Der Waals Interactions ◽  
Total Flavonoids ◽  
Hypophthalmichthys Molitrix ◽  
Diphenyltetrazolium Bromide ◽  
Health Promoting ◽  
Yellow Onion

This study focuses on combining different bioprocessing tools in order to develop an in-depth engineering approach for enhancing the biological properties of two valuable food by-products, namely fish waste and yellow onion skins, in a single new bioactive formulation. Bone tissue from phytophagous carp (Hypophthalmichthys molitrix) was used to obtain bioactive peptides through papain-assisted hydrolysis. The peptides with molecular weight lower than 3 kDa were characterized through MALDI-ToF/ToF mass spectrometry and bioinformatics tools. As a prerequisite for microencapsulation, the ability of these peptides to bind the flavonoids extracted from yellow onion skins was further tested through fluorescence quenching measurements. The results obtained demonstrate a considerable binding potency with a binding value of 106 and also the presence of one single or one class of binding site during the interaction process of flavonoids with peptides, in which the main forces involved are hydrogen bonds and van der Waals interactions. In the freeze-drying microencapsulation process, an efficiency for total flavonoids of 88.68 ± 2.37% was obtained, considering the total flavonoids and total polyphenols from the powder of 75.72 ± 2.58 quercetin equivalents/g dry weight (DW) and 97.32 ± 2.80 gallic acid equivalents/g DW, respectively. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test on the L929 cell line cultivated in the presence of different concentrations of microencapsulated samples (0.05–1.5 mg/mL) proved no sign of cytotoxicity, the cell viability being over 80% for all the samples.

BOTANICAL COMPOSITION AND PRODUCTIVITY OF PERENNIAL GRASSES AGROPHYTOCENOSES WITH TETRAPLOID MEADOW CLOVER

1970 ◽  
pp. 59-62
Author(s):  
N. I. Kasatkina ◽  
Zh. S. Nelyubina
Keyword(s):  
Dry Weight ◽  
Single Species ◽  
Lotus Corniculatus ◽  
Specific Weight ◽  
Biological Properties ◽  
Phleum Pratense ◽  
Perennial Grasses ◽  
High Yield ◽  
Botanical Composition ◽  
The Third

The biological properties of plants, their mutual relations under different growth conditions and at different periods of their life, must be known for obtaining highly productive agrophytocenoses with participation of a meadow clover (Trifolium pratense L.). Botanical composition and fodder productivity of perennial grasses in agrocenoses with participation of meadow tetraploid clover Kudesnik were studied in 2014-2017. It was revealed that in the first and second years of use the agrophytocenosis, the yield of green mass was formed due to meadow tetraploid clover, the share of its participation in the first mowing was at level of 71-87% and 64-97% respectively. Specific weight of clover in multispecies agrocenoses considerably decreased by the third year of use: in the first mowing up to 32-68%, in the second - up to 8-52%. At the same time, the percentage of long-term herbaceous grasses increased: meadow timothy (Phleum pratense L.) - up to 34-54%, eastern galega (Galéga orientális Lam.) - up to 33%, changeable alfalfa (Medicago x varia Martyn) - up to 22-54%, lotus corniculatus (Lotus corniculatus L.) - up to 14-19%. The proportion of weed admixture in single-species clover planting was 12%, in agrocenoses - 2-14%. The grass mixtures clover + timothy and clover + alfalfa + timothy were less infested by weeds. High yield of dry weight of single-species sowing of meadow tetraploid clover was obtained in the first two years of use - 7.8 and 6.5 tons / ha, respectively. By the third year of use, the productivity of clover has decreased to 2.9 t / ha. On average, for three years of use, the highest yield (6.2-6.3 t / ha) was formed by agrocenoses meadow tetraploid clover + meadow timothy and meadow tetraploid clover + changeable alfalfa + meadow timothy.

scholarly journals Advances in Processing, Quality, Safety, Authenticity, Nutrition, Health, and Oral Health of Extra Virgin Olive Oil

Proceedings ◽  
2020 ◽  
Vol 70 (1) ◽  
pp. 107
Author(s):  
Maria Antoniadou ◽  
Theodoros Varzakas
Keyword(s):  
Oral Health ◽  
Olive Oil ◽  
Review Paper ◽  
Safety Management ◽  
Virgin Olive Oil ◽  
Extra Virgin Olive Oil ◽  
Biological Properties ◽  
Management Systems ◽  
Health Promoting ◽  
Effect Of Food

Olive fruit exerts great socioeconomic impact. Many studies have shown the multiple benefits of healthy olive oil for the human body. The increase in consumption of extra virgin olive oil is mainly due to its unique sensory, nutritive qualities, biological properties, and health-promoting effects. In this review paper recent advances in quality, safety, authenticity, processing, nutrition, health, and oral health of extra virgin olive oil (EVOO) have been discussed and outlined. Regarding safety, the effect of food safety management systems in EVOO has been reported along with the use of different tools such as FMEA and Ishikawa.

Cytotoxicity of an Experimental Light-Cured Orthodontic Adhesive

2019 ◽  
Vol 294 ◽  
pp. 65-70
Author(s):  
Kanin Nimcharoensuk ◽  
Niwat Anuwongnukroh ◽  
Surachai Dechkunakorn ◽  
Vanthana Sattabanasuk ◽  
Panya Sunintaboon ◽  
...  
Keyword(s):  
Cell Viability ◽  
Cytotoxic Effect ◽  
L929 Cell ◽  
Descriptive Statistics ◽  
The Other ◽  
Cytotoxic Potential ◽  
Monomer Ratio ◽  
Diphenyltetrazolium Bromide ◽  
Good Biocompatibility ◽  
L929 Cell Line

The objective of this study was to compare the cytotoxicity of a domestically-made light-cured orthodontic adhesive to a commercial adhesive, Transbond XT (3M Unitek, USA). An in-house orthodontic adhesive composed of a filler 60-70 weight % and a monomer ratio (BisGMA:TEGDMA) of 6:4 with 0.5% of photoinitiator was mixed. The potential cytotoxic effect of this experimental and a control adhesive was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay according to ISO 10993-5: 2009(E). The L929 cell line was grown in 96-well tissue culture plates (1x105 cells/mm3). Thin cured-resin discs of each material weighing 0.4 gram were prepared and incubated for 1, 3, 5, 7, 14, and 30 days in Dulbecco’s modified Eagle medium (DMEM) at 37°C and 95% humidity with 5% CO2. The percentage of cell viability was reported by descriptive statistics. The result showed that the cell viability of the experimental adhesive was higher than Transbond XT in all measured periods. The cytotoxicity of both the adhesives gradually decreased with the progression of time. In conclusion, the in-house adhesive showed a good biocompatibility since the first day following polymerization. On the other hand, Transbond XT started with a cytotoxic potential, then, turned to be non-cytotoxic after 5 days of curing.

Magnesium substitution effect on porous scaffolds for bone repair

2011 ◽  
Vol 6 (3) ◽  
pp. 301-311 ◽  
Author(s):  
Oana Craciunescu ◽  
Christu Tardei ◽  
Lucia Moldovan ◽  
Otilia Zarnescu
Keyword(s):  
Bone Repair ◽  
Substitution Effect ◽  
Dry Weight ◽  
Collagen Type I ◽  
Biological Properties ◽  
Porous Scaffolds ◽  
Type I ◽  
Composite Scaffolds ◽  
Cross Links ◽  
Nanocomposite Scaffolds

AbstractOf great interest in developing artificial bone is the incorporation of magnesium (Mg) ions into the ceramic lattice in order to improve the physico-chemical and structural properties of the material and to increase its morphological affinity towards newly formed osseous tissue. In the present study, we evaluated the morphological and biological properties of composite scaffolds fabricated by mixing a nanopowder of Mg-substituted beta-tricalcium phosphate with collagen type I in two dry weight ratios (variant I and II). We used biochemical methods, and electron and light microscopy to investigate their porosity, biodegradability and morphology. Osteoblast cell culture behavior in the presence of nanocomposite variants was also examined. Variant I scaffold presented a higher percentage of cross-links and a better resistance to collagenase degradation compared to variant II scaffold. Their porosity did not vary significantly. Osteoblasts cultivated in the presence of nanocomposite scaffolds for 72 h exhibited good cell viability and a normal morphology. When osteoblasts were injected into the scaffolds, a slightly higher proportion of adhered cells were observed for Mg-substituted samples after 7 days of cultivation. All these results showed that Mg-containing porous composite scaffolds had controlled degradation, allowed osteoblast proliferation and adhesion and are good candidates for bone repair.

scholarly journals EVALUATION OF TOTAL PHENOLS, TOTAL FLAVONOIDS, ANTIOXIDANT, AND ANTICANCER ACTIVITY OF MUCUNA PRURIENS SEED

2018 ◽  
Vol 11 (3) ◽  
pp. 242
Author(s):  
Kavitha K
Keyword(s):  
Liver Cancer ◽  
Anticancer Activity ◽  
Cell Line ◽  
Cancer Cell Line ◽  
Ethanol Extract ◽  
Biological Properties ◽  
Seed Extract ◽  
Mucuna Pruriens ◽  
Total Flavonoids ◽  
Total Phenols

 Objective: In recent years, herbal plants have been got more attention due to their diverse presence of phytochemicals and its biological properties. Hepatocellular carcinoma is one of the major worldwide problems primarily caused by hepatitis B and C virus infection. End-stage liver cancer treatment options are limited, thus requiring expensive liver transplantation which is not available in many countries.Methods: In the present study, the Mucuna pruriens seed extract was analyzed for phytochemicals, antioxidant activity, total phenols, and total flavonoids content. The seed extract was further analyzed for its anticancer activity by culturing liver cancer cell line. The above protocols were done by standard methods.Results: The seed extract of M. pruriens revealed more number of phytochemicals in different organic solvents. 1,1- Diphenyl- 2- picrylhydrozyl scavenging activity of plant extract was more in ethanol extract (98.051±0.547) among all other solvents. The total phenols and flavonoids content in ethanol extract were 46.442±0.353 mg gallic acid equivalent/g and 2.254±0.647 mg - quercetin equivalent /g, respectively. IC50 value of 3-(4, 5-dimethythiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) analysis of Hep-2 cell line was 150 (μg/ml).Conclusion: The present study revealed about the phytochemical contents and antioxidant potential of M. pruriens seeds. Further, the MTT analysis proved that the seed extract was effective against cancer cells and also used to treat many diseases.

scholarly journals Bunias erucago L.: Glucosinolate Profile and In Vitro Biological Potential

Molecules ◽  
2019 ◽  
Vol 24 (4) ◽  
pp. 741 ◽  
Author(s):  
Ivica Blažević ◽  
Azra Đulović ◽  
Vedrana Čikeš Čulić ◽  
Franko Burčul ◽  
Ivica Ljubenkov ◽  
...  
Keyword(s):  
Degradation Products ◽  
Dry Weight ◽  
Mediterranean Area ◽  
Biological Properties ◽  
Glucosinolate Content ◽  
Cytotoxic Activities ◽  
Plant Parts ◽  
Biological Potential ◽  
Glucosinolate Profile

Bunias erucago belongs to the Brassicaceae family, which represents a forgotten crop of the Euro-Mediterranean area. The aim of the present study was to determine the glucosinolate profile in different plant parts and biological properties (antioxidant, anticholinesterase, and cytotoxic activities) of the isolates containing glucosinolate breakdown products. The chemical profiles were determined by using HPLC-PDA-MS/MS of desulfoglucosinolates and GC-MS of glucosinolate degradation products. The analysis of B. erucago showed the presence of seven glucosinolates: gluconapin (1), glucoraphasatin (2), glucoraphenin (3), glucoerucin (4), glucoraphanin (5), glucotropaeolin (6), and glucosinalbin (7). The total glucosinolate content ranged from 7.0 to 14.6 µmol/g of dry weight, with the major glucosinolate glucosinalbin in all parts. The antioxidant activity of all volatile isolates was not notable. At a tested concentration of 227 μg/mL, flower hydro-distillate (FH) showed good AChE inhibition, i.e., 40.9%, while root hydro-distillate (RH) had good activity against BChE, i.e., 54.3%. FH showed the best activity against both tested human bladder cancer cell lines, i.e., against T24 after 72 h, which have IC50 of 16.0 μg/mL, and against TCCSUP after 48 h with IC50 of 7.8 μg/mL, and can be considered as highly active. On the other hand, RH showed weak activity against tested cancer cells.

scholarly journals Plant Foods and Underutilized Fruits as Source of Functional Food Ingredients: Chemical Composition, Quality Traits, and Biological Properties

Foods ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1474 ◽  
Author(s):  
Dario Donno ◽  
Federica Turrini
Keyword(s):  
Functional Food ◽  
Biological Activities ◽  
Well Being ◽  
Food Supplement ◽  
Biological Properties ◽  
The Novel ◽  
Food Ingredients ◽  
Health Maintenance ◽  
Plant Foods ◽  
Health Promoting

Changes in lifestyle and demographics, rising consumer incomes, and shifting preferences due to advanced knowledge about the relationships between food and health contribute to generate new needs in the food supply. Today, the role of food is not only intended as hunger satisfaction and nutrient supply but also as an opportunity to prevent nutrition-related diseases and improve physical and mental well-being. For this reason, there is a growing interest in the novel or less well-known plant foods that offer an opportunity for health maintenance. Recently, interest in plant foods and underutilized fruits is continuously growing, and agrobiodiversity exploitation offers effective and extraordinary potentialities. Plant foods could be an important source of health-promoting compounds and functional food ingredients with beneficial properties: the description of the quality and physicochemical traits, the identification and quantification of bioactive compounds, and the evaluation of their biological activities are important to assess plant food efficacy as functional foods or source of food supplement ingredients.

scholarly journals Transformed Shoots of Dracocephalum forrestii W.W. Smith from Different Bioreactor Systems as a Rich Source of Natural Phenolic Compounds

Molecules ◽  
2020 ◽  
Vol 25 (19) ◽  
pp. 4533
Author(s):  
Izabela Weremczuk-Jeżyna ◽  
Paweł Lisiecki ◽  
Weronika Gonciarz ◽  
Łukasz Kuźma ◽  
Magdalena Szemraj ◽  
...  
Keyword(s):  
High Performance ◽  
Growth Index ◽  
L929 Cell ◽  
Dry Weight ◽  
Antimicrobial Activities ◽  
Multiplication Rate ◽  
Antioxidant Power ◽  
Mtt Test ◽  
Ferric Reducing Antioxidant Power ◽  
Natural Phenolic Compounds

Transformed shoots of the Tibetan medicinal plant Dracocephalum forrestii were cultured in temporary immersion bioreactors (RITA and Plantform) and in nutrient sprinkle bioreactor (NSB) for 3 weeks in MS (Murashige and Skoog) liquid medium with 0.5 mg/L BPA (N-benzyl-9-(2-tetrahydropyranyl)-adenine) and 0.2 mg/L IAA (indole-3-acetic acid). The greatest biomass growth index (GI = 52.06 fresh weight (FW) and 55.67 dry weight (DW)) was observed for shoots in the RITA bioreactor, while the highest multiplication rate was found in the NSB (838 shoots per bioreactor). The levels of three phenolic acids and five flavonoid derivatives in the shoot hydromethanolic extract were evaluated using UHPLC (ultra-high performance liquid chromatography). The predominant metabolite was rosmarinic acid (RA)—the highest RA level (18.35 mg/g DW) and total evaluated phenol content (24.15 mg/g DW) were observed in shoots grown in NSB. The NSB culture, i.e., the most productive one, was evaluated for its antioxidant activity on the basis of reduction of ferric ions (ferric reducing antioxidant power, FRAP) and two scavenging radical (O2•– and DPPH, 1,1-diphenyl-2-picrylhydrazyl radical) assays; its antibacterial, antifungal, and antiproliative potential against L929 cells was also tested (3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) test). The plant material revealed moderate antioxidant and antimicrobial activities and demonstrated high safety in the MTT test—no cytotoxicity at concentrations up to 50 mg/mL was found, and less than a 20% decrease in L929 cell viability was observed at this concentration.

scholarly journals Activity of the Recommended and Optimized Rates of Pyridate on Chickpea - Mesorhizobium mediterraneum Symbiosis

2014 ◽  
Vol 6 (1) ◽  
pp. 92-98 ◽  
Author(s):  
Mehdi PARSA ◽  
Akbar ALIVERDI ◽  
Hossein HAMMAMI
Keyword(s):  
Active Ingredient ◽  
Chenopodium Album ◽  
Dry Weight ◽  
Biological Properties ◽  
Recommended Dose ◽  
Common Lambsquarters ◽  
L Value ◽  
Symbiotic Properties ◽  
The Impact ◽  
Chickpea Cultivars

Crop-rhizobium symbiosis can be influenced by leaching of herbicides which is unavoidable after their application. Due to an adjuvant which might help to develop the low-use-rate of herbicide, an experiment was carried out to compare the impact of the recommended rate (1200 g active ingredient ha-1) and the optimized rate (282.15 g active ingredient ha-1) of pyridate on the biological properties of eight chickpea cultivars inoculated with Mesorhizobium mediterraneum, grown in pots. Based on the required rate of herbicide to give 95% control of common lambsquarters (Chenopodium album L.) value, the efficacy of pyridate improved up to 3.87-fold by adding methylated rapeseed oil to spray solution. The ‘Desi’ cultivar had significantly higher nodulation than ‘Kabuli’ cultivar. In general, toxicity of the recommended rate was higher than the optimized rate. With the exception of root dry weight, all of the measured parameters were significantly affected by the recommended rate of pyridate in varying degrees. The symbiotic properties of chickpea cultivars were affected more than 10% at the recommended dose. The reduced nodulation ranged from 29% to 73% among cultivars exposed to pyridate at the recommended dose. The ‘Desi’ cultivar was more sensitive than the ‘Kabuli’ to the recommended rate of pyridate. We may conclude that effective low-use-rate of pyridate via applying of activator adjuvants should be noted.

scholarly journals Antihypertensive and anticoagulant properties of glycosaminoglycans extracted from the sturgeon (Acipenser persicus) cartilage

2018 ◽  
Vol 31 (4) ◽  
pp. 163-169
Author(s):  
Katayoon Karimzadeh
Keyword(s):  
Anticoagulant Activity ◽  
Cetylpyridinium Chloride ◽  
Dry Weight ◽  
Biological Properties ◽  
Biologically Active ◽  
Sulfated Polysaccharides ◽  
Chloride Salt ◽  
Thrombin Time ◽  
Acipenser Persicus ◽  
Ace Inhibitory

Abstract Large amounts of valuable waste are produced during sea food processing. This has a great potential for conversion to biologically active proteins and polysaccharides. Among these compounds, sulfated polysaccharides have been considered due to their many biological properties. The present work was conducted to study anticoagulant activities and angiotensin-I converting enzyme (ACE) inhibitory effects of glycosaminoglycans (GAGs) extracted from the cartilage of sturgeon (Acipenser persicus). The enzymatic extraction of sturgeon cartilage was performed in the presence of cetylpyridinium chloride salt. The structure was characterized via electron microscope and Fourier transform infrared spectroscopy (FTIR) analysis. Herein, ACE inhibitory and anticoagulant properties of extracted GAGs were determined. The amount of GAGs was 6.8±1.3% of cartilage dry weight. GAGs showed good activity in ACE inhibitory – with a highest level of 85.7%. The derived anticoagulant activity indexes, APPT (activated partial thromboplastin time) and TT (Thrombin time) of the extracted polysaccharide showed a prolonging of clotting time, compare to control. The results of this study revealed that the cartilage extracted GAGs possess promising ACE inhibitory properties and anticoagulant effects. Thus, the product can be substituted for blood reducing drugs and antithrombotic agents at least in laboratory conditions.

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