scholarly journals Active Biopolymeric Films Inoculated with Bdellovibrio bacteriovorus, a Predatory Bacterium

Coatings ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 605
Author(s):  
Christian Mariel Sáenz-Santos ◽  
Omotayo Opemipo Oyedara ◽  
Yunia Verónica García-Tejeda ◽  
Claudia A. Romero-Bastida ◽  
Esperanza Milagros García-Oropesa ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Escherichia Coli ◽  
Ftir Spectroscopy ◽  
Sodium Alginate ◽  
Polymeric Films ◽  
Casting Method ◽  
Scanning Calorimetry ◽  
Bdellovibrio Bacteriovorus ◽  
Tapioca Starch ◽  
Scanning Electron

The objective of the present work was to evaluate novel active films made with biopolymeric matrices as carriers of a living Bdellovibrio bacteriovorus HD100 strain, a predatory bacterium with antimicrobial potentials against pathogens. Biopolymer films were prepared by a casting method using the following mixtures: collagen/sodium alginate/sorbitol (CA-S), collagen/sodium alginate/glycerol (CA-G), and tapioca starch/sodium alginate/glycerol (StA-G). The effects of the film formulations on the viability of the B. bacteriovorus was investigated by using Fourier Transform Infrared (FTIR) spectroscopy, Differential Scanning Calorimetry (DSC), and Scanning Electron Microscopy (SEM). SEM showed that Bdellovibrio bacteriovorus morphology was not altered in the polymeric films. FTIR spectroscopy provided information about the structural composition of the films. CA-S showed less reduction in the viability of B. bacteriovorus after its entrapment; thus, CA-S proved to be a better agent for the immobilization and preservation of B. bacteriovorus to enhance its predatory activities during application against Escherichia coli.

scholarly journals SODIUM ALGINATE/GELATIN MICROBEADS-INTERCALATED WITH KAOLIN NANOCLAY FOR EMERGING DRUG DELIVERY IN WILSON’S DISEASE

2019 ◽  
pp. 71-80 ◽  
Author(s):  
O. SREEKANTH REDDY ◽  
M. C. S. SUBHA ◽  
T. JITHENDRA ◽  
C. MADHAVI ◽  
K. CHOWDOJI RAO ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Drug Release ◽  
Ftir Spectroscopy ◽  
Sodium Alginate ◽  
Release Kinetics ◽  
In Vitro Release ◽  
Vitro Release ◽  
Scanning Electron

Objective: The aim of the present study was to fabricate and evaluate the drug release studies using Sodium Alginate (SA) and Gelatin (GE) microbeads intercalated with Kaolin (KA) nanoclay for sustained release of D-Penicillamine (D-PA). Methods: Sodium alginate/gelatin/Kaolin blend microbeads were prepared by an extrusion method by using glutaraldehyde (GA) as a crosslinker. The obtained microbeads were characterized by Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM) and X–ray diffraction (XRD). Drug release kinetics of the microbeads was investigated in simulated intestinal fluid (pH 7.4) at 37 °C. Results: Microbeads formation was confirmed by FTIR spectroscopy. X-RD reveals that the KA should be intercalated with the drug and also it confirms the molecular level dispersion of D-Penicillamine into microbeads. Scanning Electron Microscopy (SEM) studies reveal that the beads were in spherical shape with some wrinkled depressions on the surface. The in vitro release study indicates the D-Penicillamine released in a controlled manner. The in vitro release kinetics was assessed by Korsmeyer-Peppas equation and the ‘n’ value lies in between 0.557-0.693 indicates Non-Fickian diffusion process. Conclusion: The results suggest that the developed KA intercalated microbeads are good potential drug carrier for the controlled release of D-PA.

scholarly journals Characteristics of Reconstituted Collagen Fibers from Chicken Keel Cartilage Depends on Salt Type for Removal of Proteoglycans

Molecules ◽  
2021 ◽  
Vol 26 (12) ◽  
pp. 3538
Author(s):  
Anna Pudło ◽  
Szymon Juchniewicz ◽  
Wiesław Kopeć
Keyword(s):  
Electron Microscopy ◽  
Rheological Properties ◽  
Thermal Denaturation ◽  
Surface Structures ◽  
Scanning Calorimetry ◽  
Cartilage Extracellular Matrix ◽  
Oscillatory Rheometry ◽  
Freeze Dried ◽  
Basic Features ◽  
Scanning Electron

The aim of the presented research was to obtain reconstituted atelocollagen fibers after extraction from poultry cartilage using the pepsin-acidic method in order to remove telopeptides from the tropocollagen. Firstly, we examined the extraction of collagen from the cartilage extracellular matrix (ECM) after proteoglycans (PG) had been removed by the action of salts, i.e., NaCl or chaotropic MgCl2. Additionally, the effects of the salt type used for PG and hyaluronic acid removal on the properties of self-assembled fibers in solutions at pH 7.4 and freeze-dried matrices were investigated. The basic features of the obtained fibers were characterized, including thermal properties using scanning calorimetry, rheological properties using dynamic oscillatory rheometry, and the structure by scanning electron microscopy. The fibers obtained after PG removal with both analyzed types of salts had similar thermal denaturation characteristics. However, the fibers after PG removal with NaCl, in contrast to those obtained after MgCl2 treatment, showed different rheological properties during gelatinization and smaller diameter size. Moreover, the degree of fibrillogenesis of collagens after NaCl treatment was complete compared to that with MgCl2, which was only partial (70%). The structures of fibers after lyophilization were fundamentally different. The matrices obtained after NaCl pretreatment form regular scaffolds in contrast to the thin, surface structures of the cartilage matrix after proteoglycans removal using MgCl2.

Research on compatibility and surface of high impact bio-based polyamide

2021 ◽  
pp. 095400832110055
Author(s):  
Yang Wang ◽  
Yuhui Zhang ◽  
Yuhan Xu ◽  
Xiucai Liu ◽  
Weihong Guo
Keyword(s):  
Electron Microscopy ◽  
Mechanical Properties ◽  
Scanning Electron Microscopy ◽  
Differential Scanning Calorimetry ◽  
Crystallization Behavior ◽  
High Impact ◽  
X Ray Diffraction ◽  
Scanning Calorimetry ◽  
X Ray ◽  
Scanning Electron

The super-tough bio-based nylon was prepared by melt extrusion. In order to improve the compatibility between bio-based nylon and elastomer, the elastomer POE was grafted with maleic anhydride. Scanning Electron Microscopy (SEM) and Thermogravimetric Analysis (TGA) were used to study the compatibility and micro-distribution between super-tough bio-based nylon and toughened elastomers. The results of mechanical strength experiments show that the 20% content of POE-g-MAH has the best toughening effect. After toughening, the toughness of the super-tough nylon was significantly improved. The notched impact strength was 88 kJ/m2 increasing by 1700%, which was in line with the industrial super-tough nylon. X-ray Diffraction (XRD) and Differential Scanning Calorimetry (DSC) were used to study the crystallization behavior of bio-based PA56, and the effect of bio-based PA56 with high crystallinity on mechanical properties was analyzed from the microstructure.

scholarly journals Effect of the dispersion of the chromophore on the optical performances of polarizers from polyethylene and 5”-thio- (3-butyl)nonyl-2,2’:5’,2”-terthiophene

e-Polymers ◽  
2002 ◽  
Vol 2 (1) ◽  
Author(s):  
Andrea Pucci ◽  
Letizia Moretto ◽  
Giacomo Ruggeri ◽  
Francesco Ciardelli
Keyword(s):  
Electron Microscopy ◽  
Molecular Weight ◽  
Scanning Electron Microscopy ◽  
Differential Scanning Calorimetry ◽  
Melting Point ◽  
Drawing Ratio ◽  
Scanning Calorimetry ◽  
Affine Deformation ◽  
Ultra High Molecular Weight ◽  
Scanning Electron

AbstractA new polyethylene-compatible terthiophene chromophore, 5”-thio-(3- butyl)nonyl-2,2’:5’,2”-terthiophene, with melting point lower than 0°C was prepared and used for linear polarizers based on ultra-high-molecular-weight polyethylene (UHMWPE). Differential scanning calorimetry and scanning electron microscopy indicate that the new chromophore is dispersed uniformly in films of UHMWPE obtained by casting from solution. The films show excellent dichroic properties (dichroic ratio 30) at rather low drawing ratio (≈ 20) . Moreover, qualitative agreement is observed with the Ward pseudo-affine deformation scheme.

Synthesis and Study the Properties of a Solar Cell of Gold Nanoparticles Prepared in a Simple Chemical Way

2021 ◽  
Vol 19 (11) ◽  
pp. 66-71
Author(s):  
Maithm A. Obaid ◽  
Suha A Fadaam ◽  
Osama S. Hashim
Keyword(s):  
Electron Microscopy ◽  
Gold Nanoparticles ◽  
Chemical Method ◽  
X Ray Diffraction ◽  
Structural And Optical Properties ◽  
Casting Method ◽  
X Ray ◽  
Transmission Electron ◽  
Simple Chemical ◽  
Scanning Electron

The aim of this study is to prepare gold nanoparticles by a simple chemical method at a temperature of 70°C. The solution was dried on glass basest by Casting method, the rate of five drops per sample At a temperature 100 C. Then the structural and optical properties have been confirmed by X-ray diffraction, scanning electron microscopy (SEM) and Transmission Electron microscope (TEM), Fourier Transform Infrared Spectroscopy (FTIR) and spectrum.

scholarly journals Development of an Oxcarbazepine Solid Dispersion Using Skimmed Milk to Improve the Solubility and Dissolution Profile

2019 ◽  
Vol 11 (05) ◽  
Author(s):  
M. Shah ◽  
D. Patel
Keyword(s):  
Electron Microscopy ◽  
Solid Dispersion ◽  
X Ray Diffraction ◽  
Scanning Calorimetry ◽  
X Ray ◽  
Infra Red ◽  
Crystalline Nature ◽  
Skimmed Milk ◽  
Scanning Electron

Oxcarbazepine has low solubility and low oral bioavailability, so it’s a challenge to formulate suitable dosage form. In this present investigation, to improve the dissolution rate and solubility, skimmed milk is used as a carrier. Physical mixers were prepared using various drugs to carrier ratio and spray drying technology was used to develop solid dispersion with the carrier. Various techniques were used to characterize the solid dispersion immediately after they were made which includes differential scanning calorimetry, scanning electron microscopy, fourier transform infra- red spectroscopy, X-ray diffraction and in-vitro dissolution profiles. The differential scanning calorimetry thermograms of raw drug indicated of its anhydrous crystalline nature. In thermograms of solid dispersion, the characteristic peak was absent suggesting the change from crystalline nature to amorphous form. X-ray diffraction confirmed those results. X-ray diffraction results of raw drug showed highly intense peak characteristic of its crystalline nature where solid dispersion showed less intense, more diffused peak indicating the change in crystalline form. Fourier transforms infra-red spectroscopy studies showed there was no interaction between drug and carrier. Scanning electron microscopy support the amorphous nature of mixer. The whole formulation showed distinct enhancement in the drug release behavior and solubility. The optimum oxcarbazepine to skimmed milk ratio 1:3 enhances the in-vitro drug release by 3.5 fold and also show distinct increase in solubility. It was concluded that for improvement of solubility of poorly water soluble oxcarbazepine, skimmed milk powder as a carrier can be utilize very well.

scholarly journals SINTESIS NANOKOMPOSIT Nata de coco/TiO2/Ag DAN EFEKTIVITASNYA SEBAGAI ANTIBAKTERI TERHADAP BAKTERI Escherichia coli dan Staphylococcus aureus

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Heriyanto Tinentang ◽  
Henry F Aritonang ◽  
Harry S. J. Koleangan
Keyword(s):  
Electron Microscopy ◽  
Escherichia Coli ◽  
Scanning Electron Microscopy ◽  
Staphylococcus Aureus ◽  
Chemical Reduction ◽  
Bacterial Activity ◽  
Energy Dispersive ◽  
X Ray ◽  
Escherichia Coli Bacteria ◽  
Scanning Electron

Telah dilakukan penelitian tentang kemampuan aktivitas anti bakteri untuk bakteri Staphylococcus aureus (gram positif) dan Escherichia coli (gram negatif) dengan menggunakan nanokomposit nata de coco/TiO2, nata de coco/Ag, dan nata de coco/TiO2/Ag dengan variasi konsentrasi Ag 0,5 M; 0,6 M; 0,7 M; 0,8 M dan 0,9 M  menggunakan metode reduksi kimia. Nanopartikel tersebut dikarakterisasi menggunakan X-Ray Diffractometry (XRD), scanning electron microscopy-energy dispersive X-ray spectroscopy  (SEM-EDS) dan anti bakteri. Untuk uji aktivitas antibakteri menggunakan metode kertas cakram dan dilakukan sebanyak dua kali ulangan untuk tiap-tiap sampel dan bakteri yang diuji. Hasil penelitian menunjukan, aktivitas anti bakteri nanokomposit yang paling baik dalam menghambat pertumbuhan bakteri adalah nanokomposit Nata de coco/TiO2/Ag mampu menghambat pertumbuhan bakteri Escherichiacoli dan Staphylococcusaureus, namun nanokomposit tersebut lebih efektif menghambat pertumbuhan bakteri Escherichiacoli.ABSRACT Research on the ability of anti-bacterial activity for Staphylococcus aureus (gram positive) and Escherichia coli (gram negative) bacteria using nata de coco / nanocomposites TiO2, nata de coco / Ag, and nata de coco / TiO2 / Ag with variations of Ag 0,5 M; 0.6 M; 0.7 M; 0.8 M and 0.9 M using the chemical reduction method. Nanoparticles were characterized using X-Ray Diffractometry (XRD), scanning electron microscopy-energy dispersive X-ray spctroscopy  (SEM-EDS) and anti-bacterial actvity. Test the antibacterial activity using the paper disc method and repeated two times for each sample and bacteria tested. The results showed that the good anti-bacterial activity of nanocomposites in inhibiting bacterial growth was nanocomposite nata de coco /TiO2/Ag  able to inhibit the growth of Escherichia coli and S. aureus, but the nanocomposite is more effective in inhibiting the growth of Escherichia  coli bacteria.

Study on the Calamine/Sodium Alginate Modified Viscose Fiber

2011 ◽  
Vol 418-420 ◽  
pp. 192-195
Author(s):  
Dong Qi Liu ◽  
Ying Liu ◽  
Shu Fa Han ◽  
Yu Feng Zhang ◽  
Cui Yu Yin
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Sodium Alginate ◽  
Mechanical Performance ◽  
Viscose Fiber ◽  
Alginate Solution ◽  
Fiber Properties ◽  
Injection Methods ◽  
The Stability ◽  
Scanning Electron

In this article we successfully prepared calamine / sodium alginate viscose fiber. Good dispersion and stability of the modified solution was prepared by dispersing calamine in alkaline solution of sodium alginate, and then mixed it with viscose spinning solution by spinning injection methods. Moreover, the stability of calamine / sodium alginate solution, the effect of concentration of calamine on the fiber properties is studied in this paper. Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and physical mechanical performance are test to characterize the structure and the performance of the calamine / sodium alginate viscose fiber.

Annealing Effects in Conventionally Cast and Homogenized Al-Zn-Mg(-Sc-Zr) Alloy

2017 ◽  
Vol 380 ◽  
pp. 167-172
Author(s):  
Martin Vlach ◽  
Veronika Kodetova ◽  
Bohumil Smola ◽  
Hana Kudrnova ◽  
Tomáš Kekule
Keyword(s):  
Electron Microscopy ◽  
Supersaturated Solid Solution ◽  
Microstructure Development ◽  
Scanning Calorimetry ◽  
Zr Addition ◽  
Annealing Effects ◽  
Positive Effect ◽  
Scanning Electron ◽  
Zr Alloy ◽  
Conventionally Cast

Al-based alloys are very preferred for automotive manufacture to produce lightweight vehicles. The positive effect of Sc,Zr-addition on the mechanical properties in Al-based alloys is generally known. Microstructure, mechanical, electrical and thermal properties of the conventionally cast and homogenized Al–Zn–Mg alloy with and without Sc,Zr-addition during isochronal annealing were studied. The electrical resistometry and microhardness together with differential scanning calorimetry measurements were compared to microstructure development that was observed by optical microscopy and transmission and scanning electron microscopy. It was observed that the Sc,Zr-content in the alloy after casting is not homogeneously distributed but concentrated in randomly localized matrix regions and together with Zn and Mg in the particles at grain boundaries. However, the hardening effect after annealing above 280 °C lightly reflects the Sc,Zr-addition. The distinct changes in resistivity and microhardness as well as in heat flow of the alloys studied are mainly caused by formation/dissolution of the Guinier-Preston zones and subsequent precipitation of the metastable particles from the Al–Zn–Mg system. The eutectic Zn,Mg-containing phase partly disappeared during the annealing above ~ 390 °C. Melting of the Zn,Mg-containing phase was observed at ~ 475 °C. The decomposition sequence of the supersaturated solid solution of the studied alloys is compatible with the decomposition sequence of the Al–Zn–Mg system minimally up to ~ 380 °C.

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