scholarly journals Effects of Single or Combined Administration of Dietary Synbiotic and Sodium Propionate on Humoral Immunity and Oxidative Defense, Digestive Enzymes and Growth Performances of African Cichlid (Labidochromis lividus) Challenged with Aeromonas hydrophila

Fishes ◽  
2021 ◽  
Vol 6 (4) ◽  
pp. 63
Author(s):  
Omid Safari ◽  
Mehrdad Sarkheil ◽  
Davar Shahsavani ◽  
Marina Paolucci
Keyword(s):  
Humoral Immunity ◽  
Aeromonas Hydrophila ◽  
Digestive Enzymes ◽  
Feed Conversion ◽  
Sodium Propionate ◽  
Antioxidant Responses ◽  
African Cichlid ◽  
Humoral Immune ◽  
Randomized Design ◽  
Growth Performances

The aim of the present study was to investigate the potential effects of dietary synbiotic (SYN) (Pediococcus acidilactici + Galactooligosaccharides; 10 g kg−1), sodium propionate (SP; 5, 10 and 20 g kg−1) and a combination of SYN + SP on the growth performance, humoral immunity, antioxidant responses and disease resistance against Aeromonas hydrophila of African cichlid (Labidochromis lividus) fingerlings (0.52 ± 0.05 g) in a feeding trial lasting 63 days. A completely randomized design was run with eight treatments, including 0 (control) and supplemented diets containing SYN + SP (e.g., 10 + 5, 10 + 10, 10 + 20, 0 + 5, 0 + 10, 0 + 20 and 10 + 10). The lowest feed conversion ratio value was observed in fish fed the 5 g kg−1-SP and 10 g kg−1-SYN (p < 0.05). The highest values of protein efficiency ratio and protein productive value were recorded in fish fed the 10 g kg−1-SYN (p < 0.05). Fish fed the 10 g kg−1-SYN diet had the highest activities of immunity (lysozyme, immunoglobulin) and antioxidant responses (glutathione peroxidase and superoxide dismutase) (p < 0.05). After 28 days post-challenge, the highest survival rate (57%) was recorded in the diet containing 10 g kg−1 SYN and 5 g kg−1 SP. The results indicated that the single administration of SYN or combined with SP, especially at the level of 5 g kg−1 of diet, enhanced the survival and growth performances, humoral immune response, antioxidant and digestive enzymes of African cichlid.

scholarly journals Aeromonas hydrophila in tilapia (Oreochromis niloticus) after the intake of aflatoxins

2013 ◽  
Vol 80 (4) ◽  
pp. 400-406 ◽  
Author(s):  
Samira Teixeira Leal de Oliveira ◽  
Gisele Veneroni-Gouveia ◽  
Janio Morais Santurio ◽  
Mateus Matiuzzi da Costa
Keyword(s):  
Aeromonas Hydrophila ◽  
Experimental Unit ◽  
Control Group ◽  
Feed Conversion ◽  
Group 4 ◽  
Randomized Design ◽  
Group A ◽  
Group 5 ◽  
Group 2 ◽  
The Impact

The objectives of this study were to assess the effect of the intake of aflatoxin on the development of tilapia and to evaluate the impact of inoculation with Aeromonas hydrophila on performance parameters, so these two individual tests were performed. One hundred and twenty fingerlings aged 35 days old, with mean weight of 1.55 ± 0.005 g and mean length of 5 cm were used in each test, distributed in 20 tanks. Each experimental unit consisted of a 60 L tank with six fingerlings. In the first experiment, increasing levels of aflatoxin (0.350, 0.757, 1.177 mg.kg feed-1) were used as treatments and, for the control group, a diet without aflatoxin was used in a completely randomized design with four treatments and five replicates. In the second experiment, a control group of Nile tilapia fingerlings was used and received a diet without aflatoxin, inoculated with saline solution (group 1) and Aeromonas hydrophila (group 2), as well as groups of animals fed on diets containing 0.350 mg.kg feed-1 of aflatoxin (group 3), 0.757 mg.kg feed-1 of aflatoxin (group 4) and 1.177 mg.kg feed-1 of aflatoxin (group 5), and these groups were inoculated with Aeromonas hydrophila. The survival rate and the total length of fingerlings were influenced by the treatments (p < 0.05). The synergistic action of aflatoxins and Aeromonas hydrophila was effective and caused the death of experimental fish, thus affecting feed conversion and length.

scholarly journals Kinerja probiotik Bacillus sp. pada pendederan benih ikan lele Clarias sp. yang diinfeksi Aeromonas hydrophila

2016 ◽  
Vol 15 (2) ◽  
pp. 162 ◽  
Author(s):  
, Sukenda ◽  
Muhammad Mufthi Rafsyanzani ◽  
, Rahman ◽  
Dendi Hidayatullah
Keyword(s):  
Body Weight ◽  
Aeromonas Hydrophila ◽  
Challenge Test ◽  
Total Body Weight ◽  
Positive Control ◽  
Feed Conversion ◽  
Blood Profile ◽  
Randomized Design ◽  
Total Body ◽  
Average Body

<p class="NoParagraphStyle"><strong>ABSTRACT</strong></p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">This experiment was conducted to assess performance of <em>Bacillus</em> sp. probiotic on catfish juvenile <em>Clarias</em> sp. infected by <em>Aeromonas hydrophila</em>. The probiotic content in the diets were 0% (K+ and K-), 1%, and 2% in duplicates. This experiment used randomized design with four treatments and two replications. Juveniles with average body weight of 3.22±0.15 g/fish were reared in the 1.5×2.8×0.5 m<sup>3</sup> pond with density of 800 fish/pond. Fish were reared for 30 days and fed three times a day at rate 8% of  total body weight. At day 31, catfish were challenged by <em>A. hydrophila</em> 0.1 mL (10<sup>6</sup> cfu/mL). Post infection observation was carried out ten days with density 10 fish/aquaria. The result showed that fish fed diet containing 2% probiotic gave the best probiotic performance with survival rate of catfish 83.33% after challenged, spesific growth rate 5.40%, and 0,75 of feed conversion ratio. The results of the blood profile showed significantly better results in the treatment of probiotics compared to the positive control after challenge test <em>A. hydrophila</em>. Probiotic <em>Bacillus</em> sp. has given as much as 2% on feed provides better performance on catfish juvenile.</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Keywords: probiotic, <em>Bacillus</em> sp., <em>A. hydrophila</em>, catfish juvenille, growth</p><p class="NoParagraphStyle"><strong> </strong></p><p class="NoParagraphStyle"><strong> </strong></p><p class="NoParagraphStyle"><strong>ABSTRAK</strong></p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Penelitian ini bertujuan untuk menguji kinerja probiotik <em>Bacillus</em> sp. dalam pakan pada pendederan benih ikan lele <em>Clarias</em> sp. yang diinfeksi bakteri <em>Aeromonas hydrophila</em>. Penelitian ini menggunakan rancangan acak lengkap dengan empat perlakuan yaitu kandungan probiotik dalam pakan perlakuan yaitu 0% (K+ dan K-), 1%,  dan 2%, masing-masing dengan dua ulangan. Ikan lele yang digunakan memiliki bobot rata-rata 3,22±0,15 g/ekor, dipelihara dalam kolam terpal berukuran 1,5×2,8×0,5 m<sup>3</sup> dengan kepadatan 800 ekor/kolam. Ikan dipelihara selama 30 hari dengan frekuensi pemberian pakan tiga kali sehari sebanyak 8% dari bobot tubuh ikan. Hari ke-31 benih lele diinjeksi bakteri <em>A. hydrophila</em> dosis 0,1 mL/ekor dengan kepadatan bakteri 10<sup>6</sup> cfu/mL. Pemeliharaan setelah diinfeksi dilakukan selama sepuluh hari dengan kepadatan 10 ekor/akuarium. Hasil penelitian menunjukkan ikan yang diberi probiotik 2% memperlihatkan kinerja probiotik terbaik dengan tingkat kelangsungan hidup ikan lele sebesar 83,33% setelah diinfeksi dengan <em>A. hydrophila</em>; laju pertumbuhan harian sebesar 5,40%; dan konversi pakan 0,75. Hasil gambaran darah menunjukkan hasil yang signifikan lebih baik pada perlakuan pemberian probiotik dibandingkan kontrol positif pascauji tantang <em>A. hydrophila</em>. Probiotik <em>Bacillus </em>sp. yang diberikan sebanyak 2% pada pakan memberikan kinerja lebih baik pada pendederan benih ikan lele.</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Kata kunci: probiotik, <em>Bacillus</em> sp., <em>A. hydrophila</em>, benih lele, pertumbuhan</p><p> </p>

scholarly journals Performance and Carcass Analysis of Growing Snails Fed Calopogonium mucunoides (Calopo) And Pueraria phaseoloides (Kudzupuero)

2021 ◽  
Vol 37 (1) ◽  
pp. 85-90
Author(s):  
A. J. Omole
Keyword(s):  
Weight Gain ◽  
Feed Intake ◽  
Pueraria Phaseoloides ◽  
Feed Conversion ◽  
Feeding Trial ◽  
Highest Weight ◽  
Randomized Design ◽  
Growth Performances ◽  
Calopogonium Mucunoides ◽  
Effect Of Feeding

An experiment was carried out to determine the effect of feeding growing snails with two different leguminous plants, Calopogonium muculoides, Pueraria phaseoloides, using pawpaw (Carica papaya) leaf as control. A total of 72 growing snails (Archachatina marginata) of mean weight 74.73/3.5g were used for the feeding trial. The snails were randomly alloted into 3 different groups and each group was replicated 4 times with 6 snails per replicate in a completely randomized design. Snails in T1 were fed pawpaw leaf (control), while snails in T2 were fed Calopogolium muculoides. Parameters measured were feed intake, weight gain, shell length and width and feed conversion ratio. The feeding trial lasted for 12 weeks. The results on growth performances reveal that the highest feed intake was recorded in snails fed pawpaw leaf (PL) which was similar to the those fed Pueraria phaseoloids (PP), while the lowest feed intake was recorded in snail fed with Calopognium muculoids (CM). The highest weight gain was also recorded in snails fed with pawpaw leaf and Peuraria phaseoloids (PP) than those fed Calopognium muculoides (CM). In conclusion, Pueraria phaseoloids could be used as substitute for pawpaw leaf.

scholarly journals Increase in body weight and protein retention on meat chicken due to the addition of probiotics and digestive enzymes in fermented diet containing maggot flour and local materials

2022 ◽  
Vol 951 (1) ◽  
pp. 012107
Author(s):  
M A Yaman ◽  
U Nasution ◽  
Allaily ◽  
M Daud ◽  
Zulfan
Keyword(s):  
Body Weight ◽  
Digestive Enzymes ◽  
Digestive Enzyme ◽  
Feed Conversion ◽  
Final Body Weight ◽  
Protein Retention ◽  
Randomized Design ◽  
Fermented Feed ◽  
Significant Difference ◽  
Local Materials

Abstract The purpose of this study was to determine the growth response of meat chickens due to the addition of probiotics and digestive enzymes in the fermented diet containing maggot flour and local materials. The research was carried out at Field Laboratory of Animal Science, University of Syiah Kuala-Banda Aceh, Indonesia for 66 days. A total of 100 meat chickens were designed using a completely randomized design (CRD) consisting of 5 treatments, namely P0 = 100% fermented ration (control), P1 = 0.5% probiotic + 0% digestive enzymes + 100% fermented diet, P2 = 0% probiotic + 0.5% digestive enzymes + 100% fermented diet, P3 = 0.5% probiotic + 1% digestive enzymes + 100% fermented diet, P4 = 1% probiotic + 0.5% digestive enzymes + 100% fermented diet with 4 replications. The data obtained were analyzed using ANOVA (Analysis of variance) and significant difference of data was analyzed by Duncan’s multiple distance test. The results showed that the addition of probiotics and digestive enzymes with different levels of administration in the fermented diet had a significant effect (P<0.05) on the increase in final body weight and percentage of protein retention. The best results were obtained on chicken fed on fermented diet contained 0, 5% digestive enzymes by increasing on final body weight, protein retention, feed conversion and feed efficiency which were higher than fermented feed containing probiotics. In conclusion, it was well known that digestive enzyme effected synergistically on fermented diet in increasing protein retention resulted a higher final body weight of meat chicken.

THE EFFECT OF (Morinda citrifolia L.) MEAL IN DIET ON PERFORMANCE OF BROILER

2012 ◽  
Vol 2 (1) ◽  
pp. 21-27
Author(s):  
Yosi Fenita
Keyword(s):  
Body Weight ◽  
Body Weight Gain ◽  
Morinda Citrifolia ◽  
Feed Consumption ◽  
Feed Conversion ◽  
Randomized Design ◽  
Different Levels ◽  
Effect Of Feeding ◽  
Average Body ◽  
Average Body Weight

The objective of the research was to evaluate to effect of feeding mengkudu on performances of broilers. The research design used was completely randomized design. One hundred broilers were distributed into five treatments. The treatments were different levels of mengkudu meal (0, 0.75%, 1.5%, 2.25 % and 3%). The observed measured were feed consumption, average body weight (gain) and feed conversion. Results showed that feeding mengkudu (Morinda Citrifolia L.)  no effect significant (P>0.05) on feed consumption, average body weight and feed conversion.  In conclusion, feeding mengkudu meal up to 3% (in diet) does not negatively affect feed consumption, average body weight, and feed conversion.

The use of β-glucan extracted from baker’s yeast (Saccharomyces cerevisiae) to increase non-specific immune system and resistence of tilapia (Oreochromis niloticus) to Aeromonas hydrophila

2013 ◽  
pp. 92
Author(s):  
Ida N Jamal ◽  
Reiny A Tumbol ◽  
Remy E.P Mangindaan
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Immune System ◽  
Aeromonas Hydrophila ◽  
Phagocytic Activity ◽  
Baker’S Yeast ◽  
Baker's Yeast ◽  
Yeast Saccharomyces Cerevisiae ◽  
Randomized Design ◽  
The Difference ◽  
Motile Aeromonas Septicaemia

Motile Aeromonas Septicaemia disease (MAS) attacking tilapia has increased in recent years as a consequence of intensive aquaculture activities, which led to losses in aquaculture industry. The agent causing MAS disease is Aeromonas hydrophila. The disease can be controlled with the β-glucan. As immunostimulants, β-glucans can also increase resistance in farmed tilapia. Studies on the use of β-glucan extracted from baker's yeast Saccharomyces cerevisiae was intended to evaluate the non-specific immune system of tilapia that were challenged with Aeromonas hydrophila. The method used was an experimental method with a completely randomized design consisting of four treatments with three replicats. The dose of β-glucan used as treatments were 0 mg.kg-1 fish (Control), 5 mg.kg-1 fish (B), 10 mg.kg-1 fish (C) and 20 mg.kg-1 fish (D), each treatment as injected three times at intervals of 3 days, the injection volume of 0.5 ml/fish for nine days and resistance surveillance for seven days. The results showed that the difference in the amount of β-glucan and the frequency of the injected real influence on total leukocytes, phagocytic activity and resistance. Total leukocytes, phagocytic activity and resistance to treatment was best achieved by the administration of C a dose of  10 mg.kg-1 of the fish© Penyakit Motil Aeromonas Septicaemia (MAS) yang menyerang ikan nila mengalami peningkatan selama beberapa tahun terakhir sebagai konsekuensi dari kegiatan akuakultur intensif, yang menyebabkan kerugian dalam industri budidaya. Agen utama penyebab penyakit MAS adalah Aeromonas hydrophila. Untuk mengendalikan penyakit tersebut dapat dilakukan dengan pemberian β-glukan. Sebagai imunostimulan, β-glukan juga dapat  meningkatkan resistensi pada ikan nila yang dibudidayakan. Pengkajian mengenai pemanfaatan β-glukan yang diekstrak dari ragi roti Saccharomyces cerevisiae dimaksudkan untuk menguji sistem imun non spesifik ikan nila yang diuji tantang dengan bakteri Aeromonas hydrophila. Metode yang digunakan yaitu metode eksperimen dengan rancangan acak lengkap yang terdiri dari empat perlakuan dan tiga ulangan. Dosis β-glukan  yang digunakan sebagai perlakuan sebesar 0 mg.kg-1 ikan (Kontrol), 5 mg.kg-1 ikan (B), 10 mg.kg-1 ikan (C) dan 20 mg.kg-1 ikan (D), masing-masing perlakuan diinjeksi sebanyak 3 kali dengan interval waktu 3 hari selama 9 hari, volume injeksi 0,5 mL/ekor ikan dan pengamatan resistensi selama tujuh hari. Hasil penelitian menunjukkan perbedaan jumlah β-glukan dan frekuensi pemberian yang diinjeksikan memberikan pengaruh nyata terhadap total leukosit, aktivitas fagositosis dan resistensi. Total leukosit, aktivitas fagositosis dan resistensi terbaik dicapai pada perlakuan C dengan dosis 10 mg.kg-1 ikan©

Humoral Immunity in Heart Failure

2019 ◽  
Vol 19 (1) ◽  
pp. 14-18 ◽  
Author(s):  
Amrita Sarkar ◽  
Khadija Rafiq
Keyword(s):  
Heart Failure ◽  
Humoral Immunity ◽  
Treatment Strategies ◽  
Pathophysiological Mechanism ◽  
Peripheral Vascular ◽  
Cardiac Inflammation ◽  
Humoral Immune ◽  
Humoral Immune System ◽  
New Treatment ◽  
Immunity Function

Cardiovascular Disease (CVD) is a class of diseases that involve disorders of heart and blood vessels, including hypertension, coronary heart disease, cerebrovascular disease, peripheral vascular disease, which finally lead to Heart Failure (HF). There are several treatments available all over the world, but still, CVD and heart failure became the number one problem causing death every year worldwide. Both experimental and clinical studies have shown a role for inflammation in the pathogenesis of heart failure. This seems related to an imbalance between pro-inflammatory and anti-inflammatory cytokines. Cardiac inflammation is a major pathophysiological mechanism operating in the failing heart, regardless of HF aetiology. Disturbances of the cellular and humoral immune system are frequently observed in heart failure. This review describes how B-cells play a specific role in the heart failure states. There is an urgent need to identify novel therapeutic targets and develop advanced therapeutic strategies to combat the syndrome of HF. Understanding and describing the elements of the humoral immunity function are essential and may suggest potential new treatment strategies.

TLR4 regulates rabies virus-induced humoral immunity through recruitment of cDC2 to lymph organs

2021 ◽  
Author(s):  
Chen Chen ◽  
Chengguang Zhang ◽  
Haoqi Li ◽  
Zongmei Wang ◽  
Yueming Yuan ◽  
...  
Keyword(s):  
Immune Response ◽  
Immune Responses ◽  
Rabies Virus ◽  
Humoral Immunity ◽  
Humoral Immune Response ◽  
Critical Role ◽  
Wild Type ◽  
Humoral Immune ◽  
Specific Igg ◽  
Molecular Patterns

Rabies, caused by rabies virus (RABV), is fatal to both humans and animals around the world. Effective clinical therapy for rabies has not been achieved, and vaccination is the most effective means of preventing and controlling rabies. Although different vaccines, such as live attenuated and inactivated vaccines, can induce different immune responses, different expression of pattern recognition receptors (PRRs) also causes diverse immune responses. Toll-like receptor 4 (TLR4) is a pivotal PRR that induces cytokine production and bridges innate and adaptive immunity. Importantly, TLR4 recognizes various virus-derived pathogen-associated molecular patterns (PAMPs) and virus-induced damage-associated molecular patterns (DAMPs), usually leading to the activation of immune cells. However, the role of TLR4 in the humoral immune response induced by RABV has not been revealed yet. Based on TLR4-deficient ( TLR4 -/- ) and wild-type (WT) mouse models, we report that TLR4-dependent recruitment of the conventional type-2 dendritic cells (CD8α - CD11b + cDC2) into secondary lymph organs (SLOs) is critical for antigen presentation. cDC2-initiated differentiation of Tfh cells promotes the proliferation of germinal centre (GC) B cells, the formation of GCs, and the production of plasma cells (PCs), all of which contribute to the production of RABV-specific IgG and virus-neutralizing antibodies (VNAs). Collectively, our work demonstrates that TLR4 is necessary for the recruitment of cDC2 and for the induction of RABV-induced humoral immunity, which is regulated by the cDC2-Tfh-GC B axis. IMPORTANCE Vaccination is the most efficient method to prevent rabies. TLR4, a well-known immune sensor, plays a critical role in initiating innate immune response. Here, we found that TLR4 deficiency ( TLR4 -/- ) mice suppressed the induction of humoral immune response after immunization with rabies virus (RABV), including reduced production of VNAs and RABV-specific IgG, compared with that occurred in wild-type (WT) mice. As a consequence, TLR4 -/- mice exhibited higher mortality than WT mice after challenge with virulent RABV. Importantly, further investigation found that TLR4 signaling promoted the recruitment of cDC2 (CD8α + CD11b - ), a subset of cDCs known to induce CD4 + T cell immunity through their MHC-II presentation machinery. Our results imply that TLR4 is indispensable for an efficient humoral response to rabies vaccine, which provides new insight into the development of novel rabies vaccines.

​Effect of Hydrilla verticillata in Feed on Production Performance and Carcass Characteristics of Thai Native Chickens

2021 ◽  
Author(s):  
P. Boonkeaw ◽  
W. Ngampongsai ◽  
N. Preuksa
Keyword(s):  
Body Weight Gain ◽  
Agricultural Waste ◽  
Carcass Characteristics ◽  
Live Weight ◽  
Hydrilla Verticillata ◽  
Production Performance ◽  
Feed Conversion ◽  
Randomized Design ◽  
Native Chicken ◽  
Native Chickens

Background: Producing Thai native chickens through feed commercial is costly. To reduce the cost, native chicken could be fed with local plants or agricultural waste. Hyrrilla verticillate, a dominate macrophyte in Songkhla Lagoon, contain high crude protein and nitrogen free extract. Therefore, this research studied the use of Hydrilla verticillate in Thai native chicken diet on production performance, carcass characteristics and production cost. Methods: The experiment was executed in a completely randomized design (CRD) with 180 one-day-old commercial Thai native chicks. Four dietary treatments supplemented with 0%, 5%, 10% and 15% Hydrilla verticillata were formulated for three different phases of the experimental chickens: starter (1-4 weeks), grower (5-8 weeks) and finisher (9-12 weeks). Two chickens from each replicate were chosen for the carcass characteristics study. Result: During the starter phase, there was no notable effect shown among the chickens fed with the diet containing Hydrilla verticillata in terms of feed intake (FI) and body weight gain (BWG) (P greater than 0.05). The control chickens had best feed conversion ratio (FCR) (P less than 0.05), while the grower and the finisher periods, provided with different levels of Hydrilla verticillata, revealed no apparent differences (P greater than 0.05) concerning FI, BWG and FCR. Considering carcass characteristics, there were significant differences in live weight (LW) (P less than 0.05). But, no significant differences between treatments for hot carcass percentage, pectoralis majors, pectoralis minorstrip, thighs, wings, drumsticks and total edible carcass (P greater than 0.05). There were also statistical divergences regarding gizzard weight (GW) (P less than 0.05).

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