scholarly journals Unravelling Anti-Melanogenic Potency of Edible Mushrooms Laetiporus sulphureus and Agaricus silvaticus In Vivo Using the Zebrafish Model

2021 ◽  
Vol 7 (10) ◽  
pp. 834
Author(s):  
Aleksandar Pavic ◽  
Tatjana Ilic-Tomic ◽  
Jasmina Glamočlija
Keyword(s):  
Therapeutic Potential ◽  
Skin Pigmentation ◽  
Ethanol Extract ◽  
Dependent Manner ◽  
Zebrafish Model ◽  
Medicinal Mushrooms ◽  
Laetiporus Sulphureus ◽  
Health Promoting ◽  
High Doses

Severe drawbacks associated with the topical use of depigmenting agents in treatments of skin hyperigmentations impose a great demand for novel, effective, and safe melanogenesis inhibitors. Edible and medicinal mushrooms, known for numerous health-promoting properties, represent a rich reservoir of anti-melanogenic compounds, with the potential to be applied in preventing excessive skin pigmentation. Herein, using zebrafish (Danio rerio) as a preclinical animal model, we have demonstrated that ethanol extract of Laetiporus sulphureus (LSE) and Agaricus silvaticus (ASE) are not toxic at high doses up to 400–500 µg/mL while effectively inhibit melanogenesis in a dose-dependent manner. At depigmenting doses, the explored extracts showed no adverse effects on zebrafish embryos melanocytes. Even more, they did not provoke inflammation or neutropenia when applied at the highest dose ensuring almost complete the cells depigmentation. Since LSE and ASE have demonstrated significantly higher the therapeutic potential than kojic acid and hydroquinone, two well-known depigmenting agents, overall results of this study strongly suggest that the explored mushrooms extracts could be used as efficient and safe topical agents in treatments of skin hyperpigmentation disorders.

scholarly journals Hypolaetin-7-O-β-D-xyloside from Juniperus communis Fruits Inhibits Melanogenesis on Zebrafish Pigmentation

2017 ◽  
Vol 12 (11) ◽  
pp. 1934578X1701201
Author(s):  
Eun Ju Jeong ◽  
Jonghwan Jegal ◽  
Ki Wung Chung ◽  
Sang Gyun Noh ◽  
Hae Young Chung ◽  
...  
Keyword(s):  
Skin Pigmentation ◽  
Docking Simulation ◽  
Competitive Inhibitor ◽  
Dependent Manner ◽  
Zebrafish Model ◽  
Concentration Dependent Manner ◽  
Molecular Docking Simulation ◽  
Juniperus Communis ◽  
Skin Lightening

Hypolaetin-7- O-β-D-xyloside (1) is a naturally occurring flavonoid from Juniperus communis fruits. This study aimed to investigate anti-melanogenic effect of hypolaetin-7- O-β-D-xyloside on zebrafish pigmentation. The phenotype-based image analysis suggested that 1 suppressed the zebrafish pigmentation in a concentration-dependent manner (1-400 μM) as a reversible competitive inhibitor against the enzyme tyrosinase. According to the molecular docking simulation, the compound 1 interacted with residues His-263 of tyrosinase via hydrogen bonding. The present study provided direct experimental evidence for skin-lightening effect of 1 in an in vivo zebrafish model. Therapeutic attempts with the 1 might be useful in the management of skin pigmentation-related diseases.

Ethanol Extract of Achillea fragrantissima Enhances Angiogenesis through Stimulation of VEGF Production

2020 ◽  
Vol 21 ◽  
Author(s):  
Hana M. Hammad ◽  
Amer Imraish ◽  
Maysa Al-Hussaini ◽  
Malek Zihlif ◽  
Amani A. Harb ◽  
...  
Keyword(s):  
Wound Healing ◽  
Rural Communities ◽  
Ex Vivo ◽  
Ethanol Extract ◽  
Aortic Ring ◽  
Treated Group ◽  
Control Group ◽  
Dependent Manner ◽  
Achillea Fragrantissima

Objective: Achillea fragrantissima L. (Asteraceae) is a traditionally used medicinal herb in the rural communities of Jordan. Methods: The present study evaluated the efficacy of the ethanol extract of this species on angiogenesis in both, ex vivo using rat aortic ring assay and in vivo using rat excision wound model. Results: In concentrations of 50 and 100 µg/ml, the ethanol extract showed angiogenic stimulatory effect and significantly increased length of capillary protrusions around aorta rings of about 60% in comparison to those of untreated aorta rings. In MCF-7 cells, the ethanol extract of A. fragrantissima stimulates the production of VEGF in a dose-dependent manner. 1% and 5% of ethanol extract of A. fragrantissima containing vaseline based ointment was applied on rat excision wounds for six days and was found to be effective in wound healing and maturation of the scar. Both preparations resulted in better wound healing when compared to the untreated control group and vaseline-treated group. This effect was comparable to that induced by MEBO, the positive control. Conclusion: The results indicate that A. fragrantissima has a pro-angiogenic effect, which may act through the VEGF signaling pathway.

scholarly journals Protective Effect of Phillyrin on Lethal LPS-Induced Neutrophil Inflammation in Zebrafish

2017 ◽  
Vol 43 (5) ◽  
pp. 2074-2087 ◽  
Author(s):  
Liling Yang ◽  
Xiangjun Zhou ◽  
Weijuan Huang ◽  
Qin Fang ◽  
Jianlan Hu ◽  
...  
Keyword(s):  
Signalling Pathway ◽  
Dependent Manner ◽  
Zebrafish Model ◽  
Lps Challenge ◽  
Cell Counts ◽  
Tnf Α ◽  
Forsythia Suspensa ◽  
Anti Inflammatory

Background/Aims: Forsythia suspensa Vahl. (Oleaceae) fruits are widely used in traditional Chinese medicine to treat pneumonia, typhoid, dysentery, ulcers and oedema. Antibacterial and anti-inflammatory activities have been reported for phillyrin (PHN), the main ingredient in Forsythia suspensa Vahl fruits, in vitro. However, the underlying mechanisms in vivo remain poorly defined. In this study, we discovered that PHN exerted potent anti-inflammatory effects in lethal LPS-induced neutrophil inflammation by suppressing the MyD88-dependent signalling pathway in zebrafish. Methods: LPS-yolk microinjection was used to induce a lethal LPS-infected zebrafish model. The effect of PHN on the survival of zebrafish challenged with lethal LPS was evaluated using survival analysis. The effect of PHN on neutrophil inflammation grading in vivo was assessed by tracking neutrophils with a transgenic line. The effects of PHN on neutrophil production and migration were analysed by SB+ cell counts during consecutive hours after modelling. Additionally, key cytokines and members of the MyD88 signalling pathway that are involved in inflammatory response were detected using quantitative RT-PCR. To assess gene expression changes during consecutive hours after modelling, the IL-1β, IL-6, TNF-α, MyD88, TRIF, ERK1/2, JNK, IκBa and NF-κB expression levels were measured. Results: PHN could protect zebrafish against a lethal LPS challenge in a dose-dependent manner, as indicated by decreased neutrophil infltration, reduced tissue necrosis and increased survival rates. Up-regulated IL-1β, IL-6 and TNF-α expression also showed the same tendencies of depression by PHN. Critically, PHN significantly inhibited the LPS-induced activation of MyD88, IκBa, and NF-κB but did not affect the expression of ERK1/2 MAPKs or JNK MAPKs in LPS-stimulated zebrafish. Additionally, PHN regulated the MyD88/IκBα/NF-κB signalling pathway by controlling IκBα, IL-1β, IL-6, and TNF-α expression. Conclusion: This study provides a rationale for the clinical application of PHN as an anti-inflammatory agent.

Uncaria tomentosa (Willd. ex Schult.): focus on nutraceutical aspects

2021 ◽  
Vol 17 ◽  
Author(s):  
Amirhossein Nazhand ◽  
Alessandra Durazzo ◽  
Massimo Lucarini ◽  
Amelia M. Silva ◽  
Selma B. Souto ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Therapeutic Potential ◽  
Production Costs ◽  
Industrialized Countries ◽  
Uncaria Tomentosa ◽  
Beneficial Effects ◽  
Microbial Infections ◽  
Health Promoting

: Medicinal plants have been globally exploiting as an alternative to chemical drugs in the treatment of several diseases due to low unwanted side effects, environmentally friendly nature and low production costs, therefore, it is important to analyze the therapeutic properties of various medicinal plants to understand their potential bioactivity. Uncaria tomentosa is one of these medicinal plants with many health-promoting effects. Although the geographical resources of cat's claw go back to the remote tropics of the Amazon, industrialized countries use the plant extensively in trade. Various parts of the plants such as flowers, leaves, Stem, hook, and seed are mainly used medicinally to treat inflammation, asthma, allergies, skin impurities, microbial infections, neurodegenerative diseases, cancer, cirrhosis, gastrointestinal disorders, arthritis, heart disease, rheumatism, and fever. The end point of this review article is to prospectively scrutinize in vitro and in vivo the therapeutic potential of this plant, especially in terms of its nutritional applications and health beneficial effects.

Resveratrol: nanocarrier-based delivery systems to enhance its therapeutic potential

Nanomedicine ◽  
2020 ◽  
Author(s):  
Gurinder Singh
Keyword(s):  
Water Solubility ◽  
Therapeutic Potential ◽  
Hepatic Metabolism ◽  
Enterohepatic Recirculation ◽  
Systemic Delivery ◽  
First Pass ◽  
High Doses ◽  
Sites Of Action

Resveratrol (3,5,4′-trihydroxystilbene) is a polyphenolic compound existing in trees, peanuts and grapes and exhibits a broad spectrum of promising therapeutic activities, but it is unclear whether this entity targets the sites of action after oral administration. In vivo applicability of resveratrol has limited success so far, mainly due to its incompetent systemic delivery resulting from its low water solubility, poor bioavailability and short biological half-life. First-pass metabolism and presence of enterohepatic recirculation create doubt on the biological application of high doses typically used for in vitro trials. To augment bioavailability, absorption and uptake of resveratrol by cellular internalization, countless approaches have been implemented which involve the use of nanocarriers. Nanocarriers are a well-known delivery system used to reduce first-pass hepatic metabolism, overcome enterohepatic recirculation and accelerate the absorption of drugs via lymphatic pathways.

scholarly journals Antiobesity Effects of Extract from Spergularia marina Griseb in Adipocytes and High-Fat Diet-Induced Obese Rats

Nutrients ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 336 ◽  
Author(s):  
Yong-Hyun Park ◽  
Jae-Joon Lee ◽  
Hee-Kyoung Son ◽  
Bok-Hee Kim ◽  
Jaemin Byun ◽  
...  
Keyword(s):  
Ethanol Extract ◽  
Health Concern ◽  
Dependent Manner ◽  
High Fat ◽  
Proliferation And Differentiation ◽  
Obese Rats ◽  
Spergularia Marina ◽  
Natural Herb

Obesity has recently risen and become a serious health concern in Korea according to the westernized diet and altered lifestyle. Hence, there is a growing interest in the supplementation of phytochemicals to find a safe and effective functional ingredient to treat obesity. Spergularia marina Griseb (SM) has traditionally been used as a natural herb against chronic diseases in Korea. In this study, we investigated the antiobesity effects of SM in vitro and in vivo. SM ethanol extract (SME) inhibited proliferation and differentiation in murine adipocytes and primary porcine pre-adipocytes in a dose-dependent manner. In the in vivo study, supplementation of SM powder (SMP) remarkably attenuated fat accumulation in HFD-induced obese rats. In addition, SMP supplementation improved lipid profiles in the serum and tissues of high-fat induced obese rats. Collectively, these data indicated that SME exhibited antiobesity effects by modulating adipogenesis and lipolysis. Furthermore, SMP could be developed as an obesity-induced metabolic syndrome treatment.

scholarly journals IMMUNOMODULATORY EFFECTS OF ETHANOL EXTRACT OF CURCUMA MANGGA RHIZOMES IN MICE

2017 ◽  
Vol 10 (9) ◽  
pp. 148 ◽  
Author(s):  
Yuandani Yuandani ◽  
Edy Suwarso
Keyword(s):  
Therapeutic Potential ◽  
Ethanol Extract ◽  
Negative Control ◽  
Total Leukocyte Count ◽  
Phagocytic Index ◽  
Immunomodulatory Activity ◽  
Immunomodulatory Effects ◽  
Immunostimulatory Activity ◽  
Immunomodulatory Potential

Objective: This study was conducted to evaluate the immunomodulatory effects of ethanol extract of Curcuma mangga by in vivo study.Methods: The ethanol extract of C. mangga was comprised to carbon clearance method for its immunomodulatory potential. The extract wasadministered orally at doses of 100, 200, and 400 mg/kg BW to mice for 7 days. On day 8, carbon ink was injected, and the blood was collected formeasurement of elimination of carbon. Total leukocyte count was also determined.Results: The evaluation of immunomodulatory potential of ethanol extract of C. mangga revealed a dose-dependent increase in phagocytosis ability.The phagocytic index of ethanol extract of C. mangga was more than those of negative control, indicating the immunostimulatory activity of C. mangga.It showed low stimulation on total leukocyte count.Conclusion: The results indicate that ethanol extract of C. mangga rhizomes possesses immunomodulatory activity and has therapeutic potential forthe treatment of infectious diseases.

scholarly journals The Therapeutic Potential of Apigenin

2019 ◽  
Vol 20 (6) ◽  
pp. 1305 ◽  
Author(s):  
Bahare Salehi ◽  
Alessandro Venditti ◽  
Mehdi Sharifi-Rad ◽  
Dorota Kręgiel ◽  
Javad Sharifi-Rad ◽  
...  
Keyword(s):  
Animal Models ◽  
Health Effects ◽  
Therapeutic Potential ◽  
Dietary Assessment ◽  
Functional Activities ◽  
Formulated Diet ◽  
Naturally Occurring ◽  
Wide Range ◽  
Health Promoting

Several plant bioactive compounds have exhibited functional activities that suggest they could play a remarkable role in preventing a wide range of chronic diseases. The largest group of naturally-occurring polyphenols are the flavonoids, including apigenin. The present work is an updated overview of apigenin, focusing on its health-promoting effects/therapeutic functions and, in particular, results of in vivo research. In addition to an introduction to its chemistry, nutraceutical features have also been described. The main key findings from in vivo research, including animal models and human studies, are summarized. The beneficial indications are reported and discussed in detail, including effects in diabetes, amnesia and Alzheimer’s disease, depression and insomnia, cancer, etc. Finally, data on flavonoids from the main public databases are gathered to highlight the apigenin’s key role in dietary assessment and in the evaluation of a formulated diet, to determine exposure and to investigate its health effects in vivo.

scholarly journals Design, synthesis and biological evaluation of a series of CNS penetrant HDAC inhibitors structurally derived from amyloid-β probes

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Myeong A Choi ◽  
Sun You Park ◽  
Hye Yun Chae ◽  
Yoojin Song ◽  
Chiranjeev Sharma ◽  
...  
Keyword(s):  
Therapeutic Potential ◽  
Hdac Inhibitors ◽  
Amyloid Β ◽  
Biological Evaluation ◽  
Pharmacokinetic Studies ◽  
Dependent Manner ◽  
Reference Drug ◽  
Novel Scaffold

Abstract To develop novel CNS penetrant HDAC inhibitors, a new series of HDAC inhibitors having benzoheterocycle were designed, synthesized, and biologically evaluated. Among the synthesized compounds, benzothiazole derivative 9b exhibited a remarkable anti-proliferative activity (GI50 = 2.01 μM) against SH-SY5Y cancer cell line in a dose and time-dependent manner, better than the reference drug SAHA (GI50 = 2.90 μM). Moreover, compound 9b effectively promoted the accumulation of acetylated Histone H3 and α-tubulin through inhibition of HDAC1 and HDAC6 enzymes, respectively. HDAC enzyme assay also confirmed that compound 9b efficiently inhibited HDAC1 and HDAC6 isoforms with IC50 values of 84.9 nM and 95.9 nM. Furthermore, compound 9b inhibited colony formation capacity of SH-SY5Y cells, which is considered a hallmark of cell carcinogenesis and metastatic potential. The theoretical prediction, in vitro PAMPA-BBB assay, and in vivo brain pharmacokinetic studies confirmed that compound 9b had much higher BBB permeability than SAHA. In silico docking study demonstrated that compound 9b fitted in the substrate binding pocket of HDAC1 and HDAC6. Taken together, compound 9b provided a novel scaffold for developing CNS penetrant HDAC inhibitors and therapeutic potential for CNS-related diseases.

scholarly journals Methanol Extract ofArtemisia apiaceaHance Attenuates the Expression of Inflammatory Mediators via NF-κB Inactivation

2013 ◽  
Vol 2013 ◽  
pp. 1-12 ◽  
Author(s):  
Ji Choul Ryu ◽  
Sang Mi Park ◽  
Min Hwangbo ◽  
Sung Hui Byun ◽  
Sae Kwang Ku ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Therapeutic Potential ◽  
Interleukin 1 ◽  
Dependent Manner ◽  
Nuclear Factor ◽  
Paw Edema ◽  
Concentration Dependent Manner ◽  
Proinflammatory Mediators ◽  
Inducible Nitric Oxide

Artemisia apiaceaHance is one of the most widely used herbs for the treatment of malaria, jaundice, and dyspeptic complaint in oriental medicine. This study investigated the effects of methanol extracts ofA. apiaceaHance (MEAH) on the induction of inducible nitric oxide synthase (iNOS) and proinflammatory mediators by lipopolysaccharide (LPS) in Raw264.7 macrophage cells and also evaluated thein vivoeffect of MEAH on carrageenan-induced paw edema in rats. MEAH treatment in Raw264.7 cells significantly decreased LPS-inducible nitric oxide production and the expression of iNOS in a concentration-dependent manner, while MEAH (up to 100 μg/mL) had no cytotoxic activity. Results from immunoblot analyses and ELISA revealed that MEAH significantly inhibited the expression of cyclooxygenase-2, tumor necrosis factor-α, interleukin-1β, and interleukin-6 in LPS-activated cells. As a plausible molecular mechanism, increased degradation and phosphorylation of inhibitory-κBαand nuclear factor-κB accumulation in the nucleus by LPS were partly blocked by MEAH treatment. Finally, MEAH treatment decreased the carrageenan-induced formation of paw edema and infiltration of inflammatory cells in rats. These results demonstrate that MEAH has an anti-inflammatory therapeutic potential that may result from the inhibition of nuclear factor-κB activation, subsequently decreasing the expression of proinflammatory mediators.

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