UPLC-MS/MS Phytochemical Analysis of Two Croatian Cistus Species and Their Biological Activity

Aqueous extracts of two Cistus species wild growing in Croatia—Cistus creticus (CC) and Cistus salviifolius (CS)—have been assessed with UPLC-MS/MS, showing 43 different phytochemicals, with flavonol glycosides: myricetin-3-hexoside and myricetin-rhamnoside, predominate ones in CC and myricetin-3-hexoside in CS. Antioxidant potential tested with the FRAP method showed no difference between CS and CC aqueous extracts, while higher phenolic content of CC comparing to CS, determined with a Folin–Cicolateu reagent correlated to its higher antioxidant capacity observed by the DPPH method. Both extracts were assessed for antimicrobial activity, using disc-diffusion and broth microdilution assays, targeting the opportunistic pathogens, associated with food poisoning, urinary, respiratory tract, blood stream and wound infections in humans. Antimicrobial assays revealed that fungi were in general more sensitive to both Cistus aqueous extracts, comparing to the bacteria where two extracts showed very similar activity. The most potent activity was observed against A. baumannii for both extracts. The extracts were tested on human lung cancer (A549) cell line using the MTT assay, showing very similar antiproliferative activity. After 72 h treatment with CC and CS aqueous extracts in concentration of 0.5 g/L, the viability of the cells were 37% and 50% respectively, compared to non-treated cells.

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Antistaphylococcal Activity and Phytochemical Analysis of Crude Extracts of Five Medicinal Plants Used in the Center of Morocco against Dermatitis

International Journal of Microbiology ◽

10.1155/2019/1803102 ◽

2019 ◽

Vol 2019 ◽

pp. 1-7

Author(s):

Ikrame Zeouk ◽

Mounyr Balouiri ◽

Khadija Bekhti

Keyword(s):

Staphylococcus Aureus ◽

Current Data ◽

Bioactive Molecules ◽

Total Phenolic ◽

Phytochemical Analysis ◽

Aqueous Extracts ◽

Methicillin Resistant ◽

Antistaphylococcal Activity ◽

Cistus Salviifolius ◽

Berberis Hispanica

Novel drugs for methicillin-resistant Staphylococcus aureus (MRSA) hospital- and community-acquired infections are needed because of the emergence of resistance against antibiotics. In this study, methanolic and aqueous extracts of Berberis hispanica, Crataegus oxyacantha, Cistus salviifolius, Ephedra altissima, and Lavandula dentata selected from an ethnopharmacological study to treat skin infections in Sefrou city (Center of Morocco) were tested for their antistaphylococcal activity against strains often involved in cutaneous disorders: two methicillin-resistant Staphylococcus aureus strains and one strain of Staphylococcus epidermidis using the well-diffusion assay, while the agar macrodilution method was used to determine the minimal inhibitory concentrations. The total phenolic compounds and flavonoid contents of all tested extracts were also evaluated. Three of the five methanolic extracts showed an important antibacterial activity. Berberis hispanica extract was the most active with a minimal inhibitory concentration of 04.00 mg/ml against all tested strains, followed by Cistus salviifolius and Crataegus oxyacantha extracts containing the highest amounts of total phenols (133.83 ± 9.03 and 140.67 ± 3.17 μg equivalent of gallic acid/mg of extract). However, the aqueous extracts have not shown any activity against the tested strains. The current data suggested that the most active extracts can be a good source of natural antistaphylococcal compounds and warrants further investigations to isolate bioactive molecules.

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Evaluation for Protein Binding Affinity of Maghemite and Magnetite Nanoparticles

Journal of Nanoscience and Nanotechnology ◽

10.1166/jnn.2007.022 ◽

2007 ◽

Vol 7 (11) ◽

pp. 3706-3708 ◽

Cited By ~ 9

Author(s):

Se Chan Kang ◽

Yong Jun Jo ◽

Jong Phil Bak ◽

Ki-Chul Kim ◽

Young-Sung Kim

Keyword(s):

Protein Binding ◽

Binding Affinity ◽

Magnetite Nanoparticles ◽

Polyacrylamide Gel Electrophoresis ◽

Sodium Dodecyl ◽

Human Lung Cancer ◽

Maghemite Nanoparticles ◽

Binding Ability ◽

Sds Page ◽

Lung Cancer A549 Cell

We investigated the protein binding affinity of magnetite (Fe3O4) and maghemite (γ-Fe2O3) nanoparticles with against non-characterized protein from human lung cancer A549 cell line on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The binding ability of maghemite was 400 ng/mg. According to the SDS-PAGE results, the protein binding affinity of maghemite nanoparticles is stronger than magnetite nanoparticles. These data suggest that a protein can be detected with maghemite nanoparticles.

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Phytochemical analysis and antioxidant capacity ofTabernaemontana catharinensis A. DC. Fruits and branches

Anais da Academia Brasileira de Ciências ◽

10.1590/0001-3765201420120020 ◽

2014 ◽

Vol 86 (2) ◽

pp. 881-888 ◽

Cited By ~ 4

Author(s):

MARIANA PIANA ◽

ALINE A. BOLIGON ◽

THIELE F. DE BRUM ◽

MARINA ZADRA ◽

BIANCA V. BELKE ◽

...

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Ethyl Acetate ◽

Antioxidant Capacity ◽

Chlorogenic Acid ◽

Condensed Tannins ◽

High Performance ◽

Ethyl Acetate Fraction ◽

Phytochemical Analysis ◽

Dpph Method

The antioxidant capacity of the crude extract and fractions ofTabernaemontana catharinensis fruits and branches, was evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method and the content of polyphenols, flavonoids, alkaloids and condensed tannins were determined by the spectrophotometric method. The ethyl acetate fraction of the fruits and the n-butanol fraction of the branches showed IC50 of 181.82 µg/mL and 78.19 µg/mL, respectively. All fractions were analyzed by high performance liquid chromatography (HPLC), in the branches were quantified chlorogenic acid in the chloroform (8.96 mg/g), ethyl acetate (4.31 mg/g) and n-butanol (3.33 mg/g) fractions; caffeic acid in the ethyl acetate (5.24 mg/g) and n-butanol (1.81 mg/g); gallic acid (0.52 mg/g) in the n-butanol. In the fruits, chlorogenic acid in the chloroform (1.67 mg/g); rutin in the ethyl acetate (3.45 mg/g) and n-butanol (8.98 mg/g) fractions. The present study showed that these quantified compounds can contribute to antioxidant capacity which was higher in the branches than in the fruits.

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Phytochemical analysis and antibacterial activity of Khaya senegalensis bark extracts on Bacillus subtilis, Escherichia coli and Proteus mirabilis

International Journal of Phytomedicine ◽

10.5138/09750185.1784 ◽

2016 ◽

Vol 8 (3) ◽

pp. 333 ◽

Cited By ~ 1

Author(s):

Abdullahi Aliyu ◽

Alkali BR ◽

Yahaya MS ◽

Garba A ◽

Adeleye SA ◽

...

Keyword(s):

Escherichia Coli ◽

Antibacterial Activity ◽

Bacillus Subtilis ◽

Proteus Mirabilis ◽

Traditional Healers ◽

Phytochemical Analysis ◽

Aqueous Extracts ◽

E Coli ◽

Khaya Senegalensis ◽

Ethanol Extracts

The aqueous and ethanol extracts of the bark of Khaya senegalensis were screened for their phytochemical constituents and preliminary antibacterial activity against Bacillus subtilis, Escherichia coli and Proteus mirabilis. The minimum inhibitory concentration (MIC) of the plant on the tested organisms was determined using multiple tubes method.Alkaloids, anthraquinones, glycosides, tannins and steroids were detected in both extracts.The ethanol and aqueous extracts of the plant showed antibacterial activity against B. subtilis and E. coli, with the aqueous extracts having more activity than those of ethanol. However the growth of P. mirabilis was not inhibited by either of the extracts. The MIC value was determined to be 50 mg/ml for B. subtilis and E. coli. The results are suggestive of considerable antibacterial activity of K. senegalensis and may justify its use in the treatment of bacterial diseases by herbalists or traditional healers.

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Common Ancestry & Genetic Diversity of Few Indigenous Chilli Land Races of North East India

South Asian Journal of Experimental Biology ◽

10.38150/sajeb.7(6).p240-247 ◽

2018 ◽

Vol 7 (6) ◽

pp. 240-247

Author(s):

Minakshi Bhattacharjee ◽

Jutika Boro ◽

Swati Singha ◽

Hitesh Boro ◽

Narbu Gengla ◽

...

Keyword(s):

Genetic Diversity ◽

Antioxidant Properties ◽

Phytochemical Analysis ◽

Common Ancestry ◽

Oxidation Reactions ◽

Potential Health ◽

North East India ◽

North East ◽

Rapd Profile ◽

Dpph Method

Since chilies contain high quantities of bioactive potential compounds, they are expected to possess large number of pharmacological values like antioxi-dants, antifungal, antibacterial, anti-inflammatory, antiulcer, diuretics activi-ties and is being employed for the treatment of different ailments in the indigenous system of medicine. The current study aimed at comparative evaluation of antioxidant properties, phytochemicals and RAPD assay from extracts of 6 different types of chilli found in the states of Assam and Arunachal Pradesh of North East India. The plant extracts were screened for phyto-chemicals, antioxidant analysis and RAPD profile. Phytochemical analysis shows abundant presence of alkaloids, phenols, flavonoids, saponins etc. The antioxidants activities of all the aqueous extracts were evaluated using the DPPH method. The antioxidant activity (% oxidation inhibition) ranged from as high as 86.56% in Bhut Jolokia (Capsicum assamicum) extracts to as low as 18.77% in Badami Jolokia (Capsicum annum) extract. Chilli samples studied possess potential health benefits by inhibiting many oxidation reactions caused by free radicals. Genetic diversity in 6 chilli genotypes was analyzed by RAPD markers of the fourteen random primers among which 11 were polymorphic. The phylogenetic tree showed common ancestry and the divergence among the chilli species. Based on the current study findings, it may be concluded the RAPD technology can be a useful tool in explaining the genetic diversity among chilli genotypes.

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IN VITRO ANTIOXIDANT AND PHYTOCHEMICAL ANALYSIS OF DIFFERENT PARTS OF SIMANA LAGI APPLE ([MALUS DOMESTICA BORKH.] “SIMANA LAGI”) GROWN IN EAST JAVA-INDONESIA

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i11.20649 ◽

2017 ◽

Vol 10 (11) ◽

pp. 41

Author(s):

Irda Fidrianny ◽

Dhella Fitrani ◽

Siti Kusmardiyani ◽

Defri Rizaldy ◽

Komar Ruslan

Keyword(s):

Malus Domestica ◽

Antioxidant Activities ◽

Total Phenolic ◽

Phytochemical Analysis ◽

Scavenging Activity ◽

Flavonoid Content ◽

Malus Domestica Borkh ◽

Dpph Method ◽

Phenolic And Flavonoid ◽

Different Parts

Objectives: The goals of this research were to observe antioxidant properties from different parts of Simana lagi apple ([Malus domestica Borkh.] “Simana lagi”) using two antioxidant testing methods which were 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2’-azino-bis(3-ethylbenzthiazoline-6- sulfonic acid (ABTS) and correlation of total phenolic and flavonoid content with their inhibitory concentration 50 (IC50) of DPPH and IC50 of ABTS.Methods: Each sample was extracted by reflux using different polarity solvents. The extracts were evaporated using rotary evaporator. Antioxidant activities were tested using DPPH and ABTS assays, determination of total phenolic, and flavonoid content were carried out by ultraviolet-visible spectrophotometry and correlation with their IC50 of DPPH and IC50 of ABTS activities were analyzed by Pearson’s method.Results: The ethanolic leaves extract (LV3) of Simana lagi apple also exposed the highest total phenolic content (TPC) (13.88 g gallic acid equivalents/100 g), while the highest total flavonoid content was presented by ethyl acetate extract (LV2) (7.21 g QE/100 g). The lowest IC50 of DPPH scavenging activity 0.19 μg/ml, and the lowest IC50 of ABTS scavenging activity 0.15 μg/ml was given by ethanolic flesh extract (FL3) of Simana lagi apple. There were significantly negative correlation between TPC in all different parts extracts of Simana lagi apple with their IC50 of DPPH and IC50 of ABTS.Conclusions: All different parts extracts of Simana lagi apple were categorized as very strong antioxidant by DPPH and ABTS method (except n-hexane peels extract and n-hexane LV by DPPH method, and n-hexane FE by ABTS method). The major contributor in antioxidant activities of peels, flesh and leaves extracts of Simana lagi apple by DPPH and ABTS methods were phenolic compounds. Antioxidant activities of peels, flesh and leaves extracts of Simana lagi apple showed linear results by DPPH and ABTS methods.

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Synthesis, In Vitro Anticancer Activity and In Silico Study of some Benzylidene Hydrazide Derivatives

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.840.277 ◽

2020 ◽

Vol 840 ◽

pp. 277-283

Author(s):

Melanny Ika Sulistyowaty ◽

Galih Satrio Putra ◽

Tutuk Budiati ◽

Katsuyoshi Matsunami

Keyword(s):

Cell Line ◽

A549 Cell ◽

In Silico ◽

Protein Tyrosine Kinase ◽

Human Lung ◽

Human Lung Cancer ◽

A549 Cell Line ◽

Mtt Method ◽

Lung Cancer A549 Cell

Some benzylidenehydrazides (3a-e) have been synthesized in three reaction steps from anthranilic acid in good yields, about 70% - 99%. The structures of the synthesized compounds were analyzed using spectroscopic methods. The compounds were evaluated its activity against human lung cancer, A549 cell line by MTT method and studied its molecular docking onto the protein tyrosine kinase (PDB ID: 1M17) by using Molegro® vs. 5.5. The data showed that N-(2-(2-(4-nitrobenzylidene)hydrazinecarbonyl)phenyl)benzamide (3d) which synthesized in 70% yield and has the highest activity on inhibiting the growth of A549 cell line with IC50 10.9μg/mL, which was linier with our in silico study. Compound 3d has the smallest RS value -94.44 kcal/mol, lower than selected Ligand, Erlotinib.

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Human Pituitary Tumor Transforming Gene (hPTTG) Inhibits Human Lung Cancer A549 Cell Growth through Activation of p21WAF1/CIP1

Endocrine Journal ◽

10.1507/endocrj.50.771 ◽

2003 ◽

Vol 50 (6) ◽

pp. 771-781 ◽

Cited By ~ 21

Author(s):

Yi-Ming MU ◽

Koichi OBA ◽

Toshihiko YANASE ◽

Takeshi ITO ◽

Kenji ASHIDA ◽

...

Keyword(s):

Lung Cancer ◽

Cell Growth ◽

A549 Cell ◽

Pituitary Tumor ◽

Human Lung ◽

Human Lung Cancer ◽

Human Pituitary ◽

Transforming Gene ◽

Lung Cancer A549 Cell ◽

Human Pituitary Tumor

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Evaluation of antibacterial and antioxidant potential of Euphorbia cotinifolia linn. leaf extracts

Chemical Industry and Chemical Engineering Quarterly ◽

10.2298/ciceq120610099j ◽

2014 ◽

Vol 20 (1) ◽

pp. 19-28 ◽

Cited By ~ 4

Author(s):

B. Jayalakshmi ◽

K.A. Raveesha ◽

K.N. Amruthesh

Keyword(s):

Ascorbic Acid ◽

Ethyl Acetate ◽

Pathogenic Bacteria ◽

Antioxidant Properties ◽

Klebsiella Pneumonia ◽

Phytochemical Analysis ◽

Significant Activity ◽

Leaf Extracts ◽

Dpph Method ◽

Ic50 Values

Antibacterial activity of aqueous and solvent extracts of E. cotinifolia leaves were tested against some human pathogenic bacteria viz. Escherichia coli, Klebsiella pneumonia, Bacillus subtilis, Bacillus cereus, Salmonella typhi, Enterobacter aerogenes and Staphylococcus aureus by agar cup diffusion and broth microdilution methods. Antioxidant properties were evaluated for different solvent extracts by diphenyl picryl hydrazyl (DPPH), nitric oxide (NO) and hydrogen peroxide methods and IC50 values were calculated and compared with the standard ascorbic acid and butylated hydroxyanisole. Among the aqueous and organic solvent extracts, methanol and ethyl acetate, showed significant activity against B. subtilis and E. aerogenes which recorded a maximum inhibition zone of 17.25 mm. Minimum inhibitory concentration of methanol and ethyl acetate extracts for different bacteria ranged from 0.3- 1.25 mg/mL. In DPPH method, IC50 values of chloroform, petroleum ether, ethyl acetate and methanol were found to be 15, 17, 18 and 19 mg/mL, respectively, lesser than the standard, ascorbic acid (25 mg/mL). Phytochemical analysis of aqueous, ethyl acetate and methanol extract showed the presence of flavonoids, terpenoids, tannins and steroids. Further work is in progress to isolate the active compound(s).

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Chemical compounds contained in young and mature leaves of agarwood species Wikstroemia tenuiramis and its antioxidant properties

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d211020 ◽

2020 ◽

Vol 21 (10) ◽

Author(s):

RIDWANTI BATUBARA ◽

TENGKU ISMANELLY HANUM ◽

ODING AFFANDI ◽

HENNY SRI WAHYUNI

Keyword(s):

Antioxidant Activity ◽

Antioxidant Properties ◽

Chemical Compounds ◽

Raw Material ◽

Gas Chromatography Mass Spectrometry ◽

Phytochemical Analysis ◽

Mature Leaves ◽

Dpph Method ◽

North Sumatra ◽

Chemical Contents

Abstract. Batubara R, Hanum TI, Affandi O, Wahyuni HS. 2020. Chemical compounds contained in young and mature leaves of agarwood species Wikstroemia tenuiramis and its antioxidant properties. Biodiversitas 21: 4616-4622. The genus Wikstroemia is one of the genus of agarwood producing trees which has not been widely researched and published. One type of this genus is Wikstroemia tenuiramis Miq, which grows naturally in North Sumatra, the leaves are like other types of agarwood leaves can be used as raw material for agarwood leaves tea. A research has been conducted to determine the chemical contents contained in the young and mature leaves of agarwood plants species W. Tenuiramis. This research methods applied qualitative analysis using phytochemical screening, quantitative analysis to determine tannin content using titration method, antioxidant activity analysis using 2,2-diphenil-1-picrylhydrazil (DPPH) method, and compound tracing using Py-gas chromatography-mass spectrometry (Py-GC-MS) instruments. The qualitative phytochemical analysis results showed no difference in chemical compounds contained in both the young and mature leaves. Both the young and mature leaves contained flavonoids, triterpenoids, and tannins, and did not contain alkaloids, steroids and saponins. The young contained tannins of 1.079 ± 0.001% and the mature contained tannins of 4.645 ± 0.021%, not significant statistically. Py-GC-MS analysis showed the presence of various 30 bioactive compounds contained in both young and mature leaves. The analysis results also showed that both the young and mature leaves had very strong antioxidant activity. Our findings suggest that the two types of leaves have equal utility value of in term of chemical contents and antioxidant potentials.

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