scholarly journals Identifying Antibacterial Compounds in Black Walnuts (Juglans nigra) Using a Metabolomics Approach

Metabolites ◽  
2018 ◽  
Vol 8 (4) ◽  
pp. 58 ◽  
Author(s):  
Khanh-Van Ho ◽  
Zhentian Lei ◽  
Lloyd Sumner ◽  
Mark Coggeshall ◽  
Hsin-Yeh Hsieh ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Cost Effective ◽  
Bioactive Compound ◽  
The United States ◽  
Black Walnut ◽  
Juglans Nigra ◽  
Compound Identification ◽  
Antibacterial Compounds ◽  
Nut Production ◽  
Health Promoting

Black walnut (Juglans nigra L.) is one of the most economically valuable hardwood species and a high value tree for edible nut production in the United States. Although consumption of black walnut has been linked to multiple health-promoting effects (e.g., antioxidant, antimicrobial, anti-inflammatory), the bioactive compounds have not been systematically characterized. In addition, the associations between different black walnut cultivars and their health-promoting compounds have not been well established. In this study, the kernels of twenty-two black walnut cultivars selected for nut production by the University of Missouri Center for Agroforestry (Columbia, MO, USA) were evaluated for their antibacterial activities using agar-well diffusion assay. Among the selected cultivars, four black walnut cultivars (i.e., Mystry, Surprise, D.34, and A.36) exhibited antibacterial activity against a Gram-positive bacterium (Staphylococcus aureus), whereas other cultivars showed no effect on the inhibition of this bacterium. The antibacterial compounds showing the strongest activity were isolated with bioassay-guided purification and identified using a metabolomics approach. Six antibacterial bioactive compounds responsible for antimicrobial activity were successfully identified. Glansreginin A, azelaic acid, quercetin, and eriodictyol-7-O-glucoside are novel antibacterial compounds identified in the kernels of black walnuts. The metabolomics approach provides a simple and cost-effective tool for bioactive compound identification.

scholarly journals Kombucha Tea—A Double Power of Bioactive Compounds from Tea and Symbiotic Culture of Bacteria and Yeasts (SCOBY)

Antioxidants ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 1541
Author(s):  
Hubert Antolak ◽  
Dominik Piechota ◽  
Aleksandra Kucharska
Keyword(s):  
Bioactive Compounds ◽  
Biological Activities ◽  
Alcoholic Beverage ◽  
Antimicrobial Properties ◽  
Bioactive Compound ◽  
Acetic Acid Bacteria ◽  
Kombucha Tea ◽  
Health Promoting ◽  
Fermentation Parameters ◽  
Herb Extracts

Kombucha is a low alcoholic beverage with high content of bioactive compounds derived from plant material (tea, juices, herb extracts) and metabolic activity of microorganisms (acetic acid bacteria, lactic acid bacteria and yeasts). Currently, it attracts an increasing number of consumers due to its health-promoting properties. This review focuses on aspects significantly affecting the bioactive compound content and biological activities of Kombucha tea. The literature review shows that the drink is characterized by a high content of bioactive compounds, strong antioxidant, and antimicrobial properties. Factors that substantially affect these activities are the tea type and its brewing parameters, the composition of the SCOBY, as well as the fermentation parameters. On the other hand, Kombucha fermentation is characterized by many unknowns, which result, inter alia, from different methods of tea extraction, diverse, often undefined compositions of microorganisms used in the fermentation, as well as the lack of clearly defined effects of microorganisms on bioactive compounds contained in tea, and therefore the health-promoting properties of the final product. The article indicates the shortcomings in the current research in the field of Kombucha, as well as future perspectives on improving the health-promoting activities of this fermented drink.

scholarly journals An overview of phytochemicals and potential health-promoting properties of black walnut

RSC Advances ◽  
2020 ◽  
Vol 10 (55) ◽  
pp. 33378-33388
Author(s):  
Danh C. Vu ◽  
Trang H. D. Nguyen ◽  
Thi L. Ho
Keyword(s):  
Fatty Acids ◽  
Human Health ◽  
Bioactive Compounds ◽  
Unsaturated Fatty Acids ◽  
Black Walnut ◽  
Potential Health ◽  
Health Promoting

Black walnut contains a diverse mixture of bioactive compounds, including phenolics, phytosterols, unsaturated fatty acids, and tocopherols potentially important to human health.

scholarly journals Annual Applications of N, P, and K for Four Years Moderately Increase Nut Production in Black Walnut

HortScience ◽  
1998 ◽  
Vol 33 (6) ◽  
pp. 1011-1013 ◽  
Author(s):  
Felix Ponder ◽  
James E. Jones ◽  
Joan Haines
Keyword(s):  
Soil Fertility ◽  
Black Walnut ◽  
Juglans Nigra ◽  
Nutrient Levels ◽  
Foliar Nutrient ◽  
Nut Production

Annual applications of N, P, and K fertilizers were broadcast for 4 years around black walnut trees (Juglans nigra L.) in an upland plantation to determine their effect on nut production and foliar nutrient levels. Fertilization significantly (P = 0.05) increased nut production, treatments containing P with N and/or K being most effective. Doubling the rate of application did not produce a corresponding yield in nuts. Increases in leaf concentrations of N, P, and K were associated with increasing treatment levels of these elements. Levels of all elements tested, except P, were above deficiency levels. The modest gain in production suggests that soil fertility was not a major factor limiting nut production for trees in this study.

RELATION BETWEEN ATTACK BY XYLOSANDRUS GERMANUS (COLEOPTERA: SCOLYTIDAE) AND DISEASE SYMPTOMS IN BLACK WALNUT

1985 ◽  
Vol 117 (10) ◽  
pp. 1275-1277 ◽  
Author(s):  
B.C. Weber ◽  
J.E. McPherson
Keyword(s):  
United States ◽  
Pathogenic Fungi ◽  
The United States ◽  
Black Walnut ◽  
Juglans Nigra ◽  
Southern Illinois ◽  
Disease Symptoms ◽  
The Kuril Islands ◽  
Xylosandrus Germanus ◽  
Apparently Healthy

Xylosandrus germanus (Blandford) (Coleoptera: Scolytidae) is an ambrosia beetle that occurs in Japan, Korea, the Kuril Islands, Vietnam, Taiwan, China, Central Europe, and the United States (Nobuchi 1981). Unlike most ambrosia beetles, it will attack apparently healthy trees and those that are dying or recently dead (Weber 1982). It has been associated with plants infected with pathogenic fungi, including black walnut (Juglans nigra L.) infected with species of Fusarium (Anderson and Hoffard 1978; Kessler 1974); reported symptoms include cankers, wilting and dieback, and sprouts. We found black walnut with wilting and dieback symptoms in southern Illinois in spring 1978 and central Tennessee in spring 1980. Subsequent examinations showed that X. germanus was present and apparently closely associated with the disease symptoms because of the number of diseased trees that also contained X. germanus pinholes. Therefore, we decided to study the relation between X. germanus attack and disease symptoms in black walnut.

scholarly journals The Profile of Secondary Metabolites and Other Bioactive Compounds in Cucurbita pepo L. and Cucurbita moschata Pumpkin Cultivars

Molecules ◽  
2019 ◽  
Vol 24 (16) ◽  
pp. 2945 ◽  
Author(s):  
Bartosz Kulczyński ◽  
Anna Gramza-Michałowska
Keyword(s):  
Chemical Composition ◽  
Bioactive Compounds ◽  
Phenolic Acids ◽  
Nutritional Value ◽  
Cucurbita Pepo ◽  
Protocatechuic Acid ◽  
Bioactive Compound ◽  
Cucurbita Moschata ◽  
The World ◽  
Health Promoting

Plants and animals are sources of various bioactive compounds that exhibit a broad spectrum of health-promoting effects. Scientists continue studies on the chemical composition of many products in search of foods with high nutritional value. The pumpkin (Cucurbita sp.) is unquestionably a source of valuable nutrients. This vegetable is well-known all over the world and it is appreciated due to its high content of carotenoids, but it is still not much used in the processing industry. The aim of present study was to compare the flesh of 15 pumpkin varieties belonging to the Cucurbita pepo and C. moschata species in terms of the bioactive compound content (carotenoids, phenolic acids, flavonols, minerals and vitamins) and to demonstrate whether the variety has an effect on the chemical composition. To date, no such extensive research has been carried out in this area. The research revealed that the pumpkin pulp had high content of carotenoids. In nearly all cases lutein was the most abundant carotenoid. Numerous phenolic acids and flavonols were also identified. All the cultivars contained gallic acid, protocatechuic acid, 4-hydroxybenzoic acid, vanillic acid, chlorogenic acid, caffeic acid, and rutin. The pumpkin pulp also contained alpha- and gamma-tocopherol. No beta- or delta-tocopherol was found. Potassium, calcium, and sodium were the most abundant minerals. The research also proved that the profile of bioactive compounds in the pumpkin pulp was considerably diversified and depended on the species and cultivar

Detection of enteroviruses in marine waters by direct RT-PCR and cell culture

1995 ◽  
Vol 31 (5-6) ◽  
pp. 323-328 ◽  
Author(s):  
K. A. Reynolds ◽  
C. P. Gerba ◽  
I. L. Pepper
Keyword(s):  
Cell Culture ◽  
Polymerase Chain Reaction Amplification ◽  
Cost Effective ◽  
The United States ◽  
Water Runoff ◽  
Rt Pcr ◽  
Marine Waters ◽  
Storm Water Runoff ◽  
Routine Monitoring ◽  
Reaction Amplification

Sewage outfalls and storm water runoff introduces pathogenic human enteric viruses into marine coastal waters, which may pose a potential public health risk. Although members of the enterovirus group have been suggested as possible indicators of sewage pollution in marine waters, the lack of rapid, sensitive and cost effective methods have prevented routine monitoring in the United States. This study compared traditional cell culture and direct RT-PCR (reverse transcriptase-polymerase chain reaction) amplification for detection of an enterovirus. Poliovirus could be recovered from 100 L of artificial seawater with an average efficiency of 77%, using adsorption and elution from electronegative filters. Viruses were eluted from the filters with 1.5% beef extract for viruses (BEV) adjusted to pH 9.5 and reconcentrated by organic flocculation to a volume of 30 mL. Substances which interfered with detection by RT-PCR were removed by treatment of the concentrates with sephadex and chelex resins. Direct RT-PCR could detect 2.5 and 0.025 PFU (plaque forming units) for single (25 cycles) and double PCR (2 × 25 cycles) in 10 μL of pure culture poliovirus samples, respectively. These methods are currently being applied to assess the occurrence of enteroviruses at marine bathing beaches influenced by sewage discharges.

Flavones and Their Analogues as Bioactive Compounds – An Overview

2019 ◽  
Vol 16 (4) ◽  
pp. 377-391 ◽  
Author(s):  
B.S. Jayashree ◽  
H. Venkatachalam ◽  
Sanchari Basu Mallik
Keyword(s):  
Biological Activity ◽  
Free Radical ◽  
Bioactive Compounds ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Cost Effective ◽  
Free Radical Scavenging ◽  
Polyphenolic Compounds ◽  
Pharmacological Activities ◽  
Poor Solubility

Flavonoids constitute a large group of polyphenolic compounds that are known to have antioxidant properties, through their free radical scavenging abilities. They possess a chromone (γ- benzopyrone) moiety, responsible for eliciting many pharmacological activities. Even though, natural flavonoids are highly potent, owing to their poor solubility, they are less used. Therefore, attempts have been made to improve their stability, solubility, efficacy and kinetics by introducing various substituents on the flavone ring. For nearly the last two decades, flavones were synthesized in our laboratory by simple, convenient and cost-effective methods, with the knowledge of both synthetic and semi-synthetic chemistry. In this direction, it was considered worthwhile to present an overview on the synthesized flavonoids. This review creates a platform for highlighting various modifications done on the flavone system along with their biological activity.

scholarly journals Spring Freeze Damage of Pecan Bloom: A Review

Horticulturae ◽  
2020 ◽  
Vol 6 (4) ◽  
pp. 82
Author(s):  
Amandeep Kaur ◽  
Louise Ferguson ◽  
Niels Maness ◽  
Becky Carroll ◽  
William Reid ◽  
...  
Keyword(s):  
Tree Height ◽  
The United States ◽  
Reproductive Organs ◽  
Tree Age ◽  
The Us ◽  
Nut Production ◽  
Freeze Damage ◽  
Terminal Buds ◽  
And Function ◽  
The Impact

Pecan is native to the United States. The US is the world’s largest pecan producer with an average yearly production of 250 to 300 million pounds; 80 percent of the world’s supply. Georgia, New Mexico, Texas, Arizona, Oklahoma, California, Louisiana, and Florida are the major US pecan producing states. Pecan trees frequently suffer from spring freeze at bud break and bloom as the buds are quite sensitive to freeze damage. This leads to poor flower and nut production. This review focuses on the impact of spring freeze during bud differentiation and flower development. Spring freeze kills the primary terminal buds, the pecan tree has a second chance for growth and flowering through secondary buds. Unfortunately, secondary buds have less bloom potential than primary buds and nut yield is reduced. Spring freeze damage depends on severity of the freeze, bud growth stage, cultivar type and tree age, tree height and tree vigor. This review discusses the impact of temperature on structure and function of male and female reproductive organs. It also summarizes carbohydrate relations as another factor that may play an important role in spring growth and transition of primary and secondary buds to flowers.

scholarly journals In Vitro Bioaccessibility of Bioactive Compounds from Citrus Pomaces and Orange Pomace Biscuits

Molecules ◽  
2021 ◽  
Vol 26 (12) ◽  
pp. 3480
Author(s):  
Adriana Maite Fernández-Fernández ◽  
Eduardo Dellacassa ◽  
Tiziana Nardin ◽  
Roberto Larcher ◽  
Adriana Gámbaro ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Total Phenol Content ◽  
No Production ◽  
Food Ingredient ◽  
Potential Health ◽  
Raw264.7 Macrophages ◽  
In Vitro Bioaccessibility ◽  
Health Promoting ◽  
Orange Pomace

The present investigation aimed to provide novel information on the chemical composition and in vitro bioaccessibility of bioactive compounds from raw citrus pomaces (mandarin varieties Clemenule and Ortanique and orange varieties Navel and Valencia). The effects of the baking process on their bioaccessibility was also assessed. Samples of pomaces and biscuits containing them as an ingredient were digested, mimicking the human enzymatic oral gastrointestinal digestion process, and the composition of the digests were analyzed. UHPLC-MS/MS results of the citrus pomaces flavonoid composition showed nobiletin, hesperidin/neohesperidin, tangeretin, heptamethoxyflavone, tetramethylscutellarein, and naringin/narirutin. The analysis of the digests indicated the bioaccessibility of compounds possessing antioxidant [6.6–11.0 mg GAE/g digest, 65.5–97.1 µmol Trolox Equivalents (TE)/g digest, and 135.5–214.8 µmol TE/g digest for total phenol content (TPC), ABTS, and ORAC-FL methods, respectively; significant reduction (p < 0.05) in Reactive Oxygen Species (ROS) formation under tert-butyl hydroperoxide (1 mM)-induced conditions in IEC-6 and CCD-18Co cells when pre-treated with concentrations 5–25 µg/mL of the digests], anti-inflammatory [significant reduction (p < 0.05) in nitric oxide (NO) production in lipopolysaccharide (LPS)-induced RAW264.7 macrophages], and antidiabetic (IC50 3.97–11.42 mg/mL and 58.04–105.68 mg/mL for α-glucosidase and α-amylase inhibition capacities) properties in the citrus pomaces under study. In addition, orange pomace biscuits with the nutrition claims “no-added sugars” and “source of fiber”, as well as those with good sensory quality (6.9–6.7, scale 1–9) and potential health promoting properties, were obtained. In conclusion, the results supported the feasibility of citrus pomace as a natural sustainable source of health-promoting compounds such as flavonoids. Unfractionated orange pomace may be employed as a functional food ingredient for reducing the risk of pathophysiological processes linked to oxidative stress, inflammation, and carbohydrate metabolism, such as diabetes, among others.

scholarly journals Nitrogen Recovery from Enhanced Efficiency Fertilizers and Urea in Intensively Managed Black Walnut (Juglans nigra) Plantations

Forests ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 352
Author(s):  
Joshua L. Sloan ◽  
Francis K. Salifu ◽  
Douglass F. Jacobs
Keyword(s):  
Black Walnut ◽  
Juglans Nigra ◽  
Water Soluble ◽  
Growth And Yield ◽  
N Recovery ◽  
Competing Vegetation ◽  
Fertilizer N Recovery ◽  
Coated Urea ◽  
Intensively Managed ◽  
Polymer Coated Urea

Intensively managed forest plantations often require fertilization to maintain site fertility and to improve growth and yield over successive rotations. We applied urea-based “enhanced-efficiency fertilizers” (EEF) containing 0.5 atom% 15N at a rate of 224 kg N ha−1 to soils under mid-rotation black walnut (Juglans nigra L.) plantations to track the fate of applied 15N within aboveground ecosystem components during the 12-month period after application. Treatments included Agrotain Ultra (urea coated with a urease inhibitor), Arborite EC (urea coated with water-soluble boron and phosphate), Agrium ESN (polymer-coated urea), uncoated urea, and an unfertilized control. Agrotain Ultra and Arborite EC increased N concentrations of competing vegetation within one month after fertilization, while neither Agrium ESN nor uncoated urea had any effect on competing vegetation N concentrations during the experiment. Agrotain Ultra and Arborite EC increased δ15N values in leaves of crop trees above those of controls at one and two months after fertilization, respectively. By contrast, Agrium ESN and uncoated urea had no effect on δ15N values in leaves of crop trees until three months after fertilization. Fertilizer N recovery (FNR) varied among ecosystem components, with competing vegetation acting as a sink for applied nutrients. There were no significant differences in FNR for all the urea-based EEF products compared to uncoated urea. Agrium ESN was the only EEF that exhibited controlled-release activity in this study, with other fertilizers behaving similarly to uncoated urea.

Sign in / Sign up

Export Citation Format

Share Document