EVALUATION OF IN VTRO ANTI BACTERIAL ACTIVITY OF THE BREYNIA PATENS (ROXB.) BENTH. & HOOK. F. EXTRACTS

The plants provide a source of inspiration for novel drug compounds, as plant derived medicines made large contributions to human health and well being. The Present investigation is about the antibacterial activity of Breynia patens (Roxb.) Benth. & Hook.f. against five bacterial strains using the disc diffusion agarmethod. The whole plant extracted with ethanol, chloroform, petroleum ether and Aqueous. The sensitivity order of the Methanol crude extracts was illustrated by the corresponding inhibition zone diameter to be Salmonella paratyphii(20±009mm zone of incubation) and followed by other bacterial strains. The resultsindicated that the plant extract exhibited antibacterial properties, thus justifying scientifically their traditional uses as medicinal plants

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Prenylated Flavonoids and C-15 Isoprenoid Analogues with Antibacterial Properties from the Whole Plant of Imperata cylindrica (L.) Raeusch (Gramineae)

Molecules ◽

10.3390/molecules26164717 ◽

2021 ◽

Vol 26 (16) ◽

pp. 4717

Author(s):

Romeo Nago ◽

Paul Nayim ◽

Armelle Mbaveng ◽

James Mpetga ◽

Gabin Bitchagno ◽

...

Keyword(s):

Silica Gel ◽

Methanol Extract ◽

Antibacterial Properties ◽

Imperata Cylindrica ◽

Drug Resistant ◽

Bacterial Strains ◽

Prenylated Flavonoids ◽

Whole Plant ◽

Crude Methanol Extract ◽

Potential Origin

The local botanical Imperata cylindrica in Cameroon was investigated for its antibacterial potency. The methanol extract afforded a total of seven compounds, including five hitherto unreported compounds comprising three flavonoids (1–3) and two C-15 isoprenoid analogues (4 and 5) together with known derivatives (6 and 7). The novelty of the flavonoids was related to the presence of both methyl and prenyl groups. The potential origin of the methyl in the flavonoids is discussed, as well as the chemophenetic significance of our findings. Isolation was performed over repeated silica gel and Sephadex LH-20 column chromatography and the structures were elucidated by (NMR and MS). The crude methanol extract and isolated compounds showed considerable antibacterial potency against a panel of multi-drug resistant (MDR) bacterial strains. The best MIC values were obtained with compound (2) against S. aureus ATCC 25923 (32 µg/mL) and MRSA1 (16 µg/mL).

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Polysaccharide-Based Film of Cassia fistula and it's Antibacterial Activity

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.875-877.87 ◽

2014 ◽

Vol 875-877 ◽

pp. 87-90 ◽

Cited By ~ 1

Author(s):

Chutimon Satirapipathkul ◽

Tanakan Chatdum

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Antibacterial Properties ◽

Water Vapor Permeability ◽

Inhibition Zone ◽

Vapor Permeability ◽

Glycerol Concentration ◽

Bacterial Strains ◽

Cassia Fistula

The film-forming potential of isolate of seed polysaccharide fromCassia fistulawas investigated. Increasing the glycerol concentration in the film increased elongation at break, film solubility and water vapor permeability but decreased tensile strength (TS). The film impregnated with the acetone extract ofAtractylodes lanceawas assessed for inhibition ofEscherichia coliandStaphylococcus aureus. The obtained results showed that the films exhibited antibacterial activity against both bacterial strains. Disc-diffusion assay revealed that the film resulted in a larger inhibition zone around the film onStaphylococcus aureusthan it did onEscherichia coliat the same extract concentrations (0.1 to 0.9 wt %). It can be seen that the film has satisfactory physical and antibacterial properties.

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Antibacterial properties of an Indian traditional medicinal plant, Rhynchosia scarabaeoides (L.) DC. (Fabaceae)

Alternative Medicine Studies ◽

10.4081/ams.2011.e17 ◽

2011 ◽

Vol 1 (1) ◽

pp. 17 ◽

Cited By ~ 1

Author(s):

Surekha Challa ◽

Kiran K. Rajam ◽

Vishnu V. V. Satyanarayana Kasapu ◽

Suresh Kumar Tanneeru ◽

Venkata Siva Satyanarayana Kantamreddi

Keyword(s):

Ethyl Acetate ◽

Bacterial Infections ◽

Antibacterial Properties ◽

Inhibition Zone ◽

Antibacterial Activities ◽

Traditional Healers ◽

Diffusion Method ◽

Bacterial Strains ◽

Plant Parts ◽

Ethanol Extracts

Rhynchosia scarabaeoides (L.) DC plant parts are extensively used by traditional healers in India to treat a variety of bacterial diseases, such as dysentery, diarrohea and skin disorders. This article reports the antibacterial activities of n-hexane, ethyl acetate and ethanol extracts belonging to the leaf, stem and root parts of R. scarabaeoides against five bacterial strains, Bacillus subtilis, Escherichia coli, Klebseilla pneumonia, Proteus vulgaris and Staphylococcus aureus, using an agar gel diffusion method. The range of inhibition zone (IZ) was found to be 15-24 mm and the minimum inhibitory activity (MIC) was found to be 1 mL/well. The IZ was found to be higher in ethyl acetate extracts while this was moderate in ethanol extracts, and no activity was seen with n-hexane extracts or root extracts. The MIC value of leaf ethyl acetate extract was found to be 1 mg against bacterial strains P. vulgaris and S. aureus, whereas 2 mg was found against B. subtilis, K. pneumoniae and E. coli. These results support the traditional usage of R. scarabaeoides plant parts in the treatment of bacterial infections. Interestingly, this plant was screened for antibacterial activity for the first time and was found to be active. Detailed chemical investigations are, therefore, warranted.

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CRUDE EXTRACTS OF MAGNOLIA ALBA FLOWERS USING N-HEXANE AND METHANOL SOLVENTS AND THEIR ANTI-ACNE POTENTIAL

Journal of medical pharmaceutical and allied sciences ◽

10.22270/jmpas.v10i6.1607 ◽

2021 ◽

Vol 10 (6) ◽

pp. 3789-3793

Author(s):

Sofia Munira

Keyword(s):

Propionibacterium Acnes ◽

Methanol Extract ◽

Antibacterial Properties ◽

Inhibition Zone ◽

Positive Control ◽

Hexane Extract ◽

Diffusion Method ◽

Disc Diffusion ◽

Disc Diffusion Method ◽

Bacterial Inhibition

This study aimed to test the antibacterial activity of n-hexane and methanol extracts of white cempaka flowers (Magnolia alba) against Propionibacterium acnes bacteria. The extract was obtained by using the maceration method for 72 hours. Each extract was tested for its antibacterial properties using the disc diffusion method with the variant concentration of the extract used was 0.05%; 2%; 4%; 6%; and 8%. The results showed that the methanol extract had a higher inhibition zone than the n-hexane extract. In n-hexane extract, the lowest concentration of 0.05% showed an inhibition zone of 6.6 mm and the highest concentration of 8% had an inhibition zone of 9.6 mm. Whereas in methanol extract, the inhibition zone obtained was 7.3 mm at a low concentration of 0.05%, but at the highest concentration of 8% the inhibition zone obtained was 11.6 mm. The effect of extract concentration on the diameter of the inhibition zone formed is very significant (directly proportional), where the higher the concentration used, the larger the diameter of the inhibition zone produced. Antibacterial testing for both extracts showed a weak (< 10 mm) to moderate (> 10 mm) bacterial inhibition, which was similar with the inhibition zone obtained from the positive control (11-11.3 mm; moderate bacterial inhibition).

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Formulation and Antibacterial Activity of Liquid Soap Containing Ketapang (Terminalia catappa L.) Leaves Extract

Borneo Journal of Pharmacy ◽

10.33084/bjop.v4i1.1589 ◽

2021 ◽

Vol 4 (1) ◽

pp. 43-50

Author(s):

Asiska Permata Dewi ◽

Dini Mardhiyani

Keyword(s):

Antibacterial Activity ◽

Staphylococcus Epidermidis ◽

Foam Stability ◽

Inhibition Zone ◽

Diffusion Method ◽

Disc Diffusion ◽

Liquid Soap ◽

Disc Diffusion Method ◽

Inhibition Zone Diameter ◽

Terminalia Catappa

Ketapang (Terminalia catappa L.) is traditionally used by the community to treat infections of the skin caused by bacteria or fungi. In this study, T. catappa leaves extract was added to the liquid soap formula as an antibacterial. The purpose of this study was to determine the secondary metabolite compounds contained in T. catappa leaves extract, physical evaluation of the preparation, and antibacterial activity of liquid soap. Liquid soap formula is made with various concentrations of T. catappa leaves extract F0 (0%), F1 (1%), F2 (2%), and F3 (3%). The resulting soap was evaluated for organoleptic, pH, high foam, homogeneity, irritation, and its activity against Staphylococcus aureus, Staphylococcus epidermidis, and Escherichia coli using the disc diffusion method. The results showed that the T. catappa leaves extract contained flavonoids, tannins, saponins, and triterpenoids. The liquid soap formula F0 is clear, while F1, F2, and F3 have the characteristics of brown-dark brown, homogeneous, pH between 4.6-5.2, foam stability between 67-72%, which is not significantly different and stable after five minutes of testing, and it does not irritate the skin. Terminalia catappa leaves extracts liquid soap has antibacterial activity at a concentration of 1%, 2%, and 3%, with the largest inhibition zone diameter produced by S. aureus.

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Antibacterial Activity of Ethanolic Extract of Sangkareho (Callicarpa longifolia Lam.) against Staphylococcus epidermidis

Borneo Journal of Pharmacy ◽

10.33084/bjop.v1i2.427 ◽

2018 ◽

Vol 1 (2) ◽

pp. 85-88 ◽

Cited By ~ 2

Author(s):

Susi Novaryatiin ◽

Asma'ul Ayuriska Sari ◽

Evi Mulyani

Keyword(s):

Antibacterial Activity ◽

Traditional Medicine ◽

Staphylococcus Epidermidis ◽

Skin Disease ◽

Ethanolic Extract ◽

Inhibition Zone ◽

Disc Diffusion ◽

Central Kalimantan ◽

Inhibition Zone Diameter ◽

Diffusion Technique

One of the plants believed as traditional medicine by the Dayak tribes in Central Kalimantan was Sangkareho (Callicarpa longifolia Lam.). This plant was usually used as traditional medicine by society in Puruk Cahu, Murung Raya Regency, Central Kalimantan and empirically believed could cure skin disease such as wound infection. This study was aimed to determine the antibacterial activity of ethanolic extract of Sangkareho (Callicarpa longifolia Lam). Antibacterial activity of ethanolic extract of Sangkareho was performed using disc diffusion technique, with four variations of concentration of 1%, 5%, 10%, and 15%. In this present study showed that ethanolic extract of Sangkareho has antibacterial activity against Staphylococcus epidermidis, wherein the resulting inhibition zone diameter was 7.3�0.8 mm, 8.3�1.2 mm, 10.5�0.5 mm, and 12.2�0.7 mm, respectively. Further research is needed to obtain an antibacterial activity of the fraction of Sangkareho.

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Uji Antibakteri Sediaan Masker Peel Off Ekstrak Etanol Daun Alpukat (Persea ameicana Mill.) terhadap Bakteri Staphylococcus epidermidis sebagai Antijerawat

Jurnal MIPA ◽

10.35799/jmuo.8.3.2019.25773 ◽

2019 ◽

Vol 8 (3) ◽

pp. 101

Author(s):

Esterlina Aldora Puluh ◽

Hosea Jaya Edy ◽

Jainer Pasca Siampa

Keyword(s):

Antibacterial Activity ◽

Staphylococcus Epidermidis ◽

Leaf Extract ◽

Antibacterial Properties ◽

Ethanol Extract ◽

Inhibition Zone ◽

Persea Americana ◽

Inhibition Zone Diameter ◽

Inhibitory Ability ◽

Activity Test

Daun Alpukat memiliki kandungan antara lain saponin, alkaloid, flavonoid, polifenol, quersetin yang bersifat sebagai antibakteri. Penelitian ini bertujuan untuk mendapatkan konsentrasi terbaik dari ekstrak etanol daun alpukat (Persea americana Mill.) yang diformulasikan sebagai sediaan masker gel peel off berdasarkan uji aktivitas antibakteri terhadap bakteri Staphylococcus epidermidis. Penelitian ini menggunakan metode ekperimental laboratorium. Formula sediaan masker gel peel off ekstrak daun Alpukat dibuat dengan variasi konsentrasi 0.1%; 0.15%; 0.2%; 0.25% dan 0.3%. Ekstrak tanaman daun alpukat diperoleh dengan cara maserasi menggunakan pelarut etanol 96%. Pengujian antibakteri dengan metode sumuran diameter zona hambat yang paling besar yaitu pada konsentrasi 0,3% dengan diameter zona hambat 3 mm ± 0,32 dan kemampuan daya hambat dikategorikan lemah. Dapat disimpulkan bahwa konsentrasi terbaik dari ekstrak etanol daun Alpukat (Persea americana Mill.) yang diformulasikan sebagai sediaan masker gel peel off yaitu pada konsentrasi 0.3% dan dikategorikan kemampuan daya hambat lemahAvocado leaves contains saponins, alkaloids, flavonoids, polyphenols, quercetin which are as antibacterial properties. This study aims to obtain the best concentration of ethanol extract of avocado leaves (Persea americana Mill.) which is formulated as a gel peel off mask preparation based on antibacterial activity test against Staphylococcus epidermidis bacteria. This study uses a laboratory experimental method. Avocado leaf extract peel off mask gel formula is made with a concentration variation of 0.1%; 0.15%; 0.2%; 0.25% and 0.3%. Avocado leaf extract is obtained by maceration using 96% ethanol solvent. Antibacterial testing with the largest inhibition zone diameter wells method is at a concentration of 0.3% with a diameter of inhibition zone 3 mm ± 0.32 and the ability of inhibition is categorized as weak. It can be concluded that the best concentration of ethanol extract of avocado leaves (Persea americana Mill.) Which is formulated as a gel peel off mask preparation is at a concentration of 0.3% and categorized as weak inhibitory ability

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Automatic Inhibition Zone Diameter Measurement for Disc Diffusion Test Using Image Segmentation

IETE Journal of Research ◽

10.1080/03772063.2021.1969293 ◽

2021 ◽

pp. 1-18

Author(s):

B. Keerthi Priya ◽

D. Akhila Reddy ◽

A. Daisy Rani ◽

Neelima Kalahasthi ◽

Wasim Ghder Soliman ◽

...

Keyword(s):

Image Segmentation ◽

Inhibition Zone ◽

Disc Diffusion ◽

Diameter Measurement ◽

Diffusion Test ◽

Inhibition Zone Diameter ◽

Zone Diameter

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Gram-scale synthesis of splat-shaped Ag–TiO2 nanocomposites for enhanced antimicrobial properties

Beilstein Journal of Nanotechnology ◽

10.3762/bjnano.11.96 ◽

2020 ◽

Vol 11 ◽

pp. 1119-1125

Author(s):

Mohammad Jaber ◽

Asim Mushtaq ◽

Kebiao Zhang ◽

Jindan Wu ◽

Dandan Luo ◽

...

Keyword(s):

Antibacterial Activity ◽

Antibacterial Properties ◽

Antimicrobial Properties ◽

Inhibition Zone ◽

Multidrug Resistant ◽

Bacterial Strains ◽

Gram Negative ◽

E Coli ◽

Contagious Diseases ◽

Good Biocompatibility

The control over contagious diseases caused by pathogenic organisms has become a serious health issue. The extensive usage of antibiotics has led to the development of multidrug-resistant bacterial strains. In this regard, metal-oxide-based antibacterial nanomaterials have received potential research interest due to the efficient prevention of microorganism growth. In this study, splat-shaped Ag–TiO2 nanocomposites (NCs) were synthesized on the gram scale and the enhanced antibacterial properties of TiO2 in the presence of silver were examined. The formation of Ag–TiO2 NCs was analyzed through various characterization techniques. The cell viability experimental results demonstrated that the Ag–TiO2 NCs have good biocompatibility. The antibacterial activity of the prepared Ag–TiO2 NCs was tested against the Gram-positive Staphylococcus aureus (S. aureus) and Gram-negative Escherichia coli (E. coli) bacterial strains. The Ag–TiO2 NCs exhibited promising and superior antibacterial properties compared to TiO2 nanospheres as confirmed by the bacterial growth and inhibition zone. The improvement in the antibacterial activity was attributed to the synergistic effect of the hybrid nature of TiO2 nanoparticles in the presence of Ag.

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Physico-chemical characterization and antibacterial activity of different types of honey tested on strains isolated from hospitalized patients

Journal of Apicultural Science ◽

10.1515/jas-2016-0013 ◽

2016 ◽

Vol 60 (1) ◽

pp. 5-18 ◽

Cited By ~ 1

Author(s):

Lia M. Junie ◽

Mihaela L. Vică ◽

Mirel Glevitzky ◽

Horea V. Matei

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antibacterial Activity ◽

Bacterial Species ◽

Antibacterial Properties ◽

Inhibition Zone ◽

Diffusion Method ◽

Bacterial Strains ◽

Disk Diffusion Method ◽

Physico Chemical

AbstractThe first aim of the study was to compare the antibacterial activity of several types of honey of different origins, against some bacterial resistant strains. The strains had been isolated from patients. The second aim was to discover the correlations between the antibacterial character of honey and the physico-chemical properties of the honey. Ten honey samples (polyfloral, linden, acacia, manna, and sunflower) from the centre of Romania were tested to determine their antibacterial properties against the following bacterial species: Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis, Salmonella enterica serovar Typhimurium, Bacillus cereus, Bacillus subtilis, and Listeria monocytogenes. Bacterial cultures in nutrient broth and the culture medium Mueller-Hinton agar were used. The susceptibility to antibiotics was performed using the disk diffusion method. All honey samples showed antibacterial activity on the isolated bacterial strains, in particular polyfloral (inhibition zone 13-21 mm in diameter) - because it is the source of several plants, and manna (inhibition zone 13-19.5 mm in diameter), and sunflower (inhibition zone 14-18.5 mm in diameter). Pure honey has a significant antibacterial activity against some bacteria which are resistant to antibiotics. Bacterial strains differed in their sensitivity to honeys. Pseudomonas aeruginosa and Staphylococcus aureus were the most sensitive. The present study revealed that honey antibacterial activity depends on the origin of the honey. We also found that there was a significant correlation between antibacterial activity of honeys and the colour of the honey but not between acidity and pH. The statistical analysis showed that the honey type influences the antibacterial activity (diameter of the bacterial strains inhibition zones).

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