scholarly journals Soil Bacteria to Regulate Phoebe bournei Seedling Growth and Sustainable Soil Utilization under NPK Fertilization

Plants ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1868
Author(s):  
Zhi-Jian Yang ◽  
Xiao-Hui Wu ◽  
Lan-Ming Huang ◽  
Wei-Wei Xie ◽  
Yu Chen ◽  
...  

Soil bacteria play a key role in the plant–soil system and can regulate the growth of Phoebe bournei seedlings under fertilization. However, there are few reports on how soil bacteria respond to fertilization and regulate seedling growth. This study adopted the “3414” field fertilization experiment, combined with soil microbial sequencing, nutrient contents, and biomass measurement, to explore the changes of soil chemical properties and bacterial structure under different NPK fertilization conditions and to establish the coupling relationship between soil bacteria, soil nutrients, and plant growth. The results showed that NPK fertilization decreased soil pH; increased soil N, P, and K content; reduced bacterial diversity and abundance; promoted the growth of dominant bacterial species; and enhanced Phoebe bournei seedlings’ soil N, P, and K elements. NPK fertilization promoted Proteobacteria growth, especially of three genera (Methylobacterium, Sphingobium, and Acinetobacter) and Actinobacteria, while it decreased Acidobacteria and Chloroflexi. By reducing the ratio of N to K and increasing P, NPK fertilization can slow soil acidification, promote bacterial reproduction, maintain P. bournei seedlings’ soil ecological stability, and balance the seedlings’ growth and sustainable soil utilization. AD3, Pseudomonas, and Rhodanobacter can be used as the marker species for N, P, and K fertilization, respectively, while Methylobacterium, Brevundimonas, Acinetobacter, and Sphingobium can be used as indicator species for soil pH and soil N, P, and K content changes, respectively. These results provided a theoretical basis and technical guidance for the effective fertilization and cultivation of robust P. bournei seedlings.

2001 ◽  
Vol 81 (1) ◽  
pp. 85-92 ◽  
Author(s):  
T E Staples ◽  
K. C. J. Van Rees

The disposal of wood ash and/or pulp and paper mill sludges is becoming increasingly more challenging as landfills are more difficult to site due to diminishing land availability and public opposition, as well as more costly to construct and operate because of increasingly stringent regulations. The most notable alternative to disposal that is receiving the attention of the forest industry is land application. The objective of this study, therefore, was to determine the influence of applying a pulp mill wood/sludge ash mixture on 1) various soil chemical properties of an Orthic Gray Luvisol and 2) the growth and nutrient uptake of white spruce [Picea glauca (Moench) Voss] seedlings. The pulp mill wood/sludge ash mixture from an olivine burner was surface broadcast at rates of 1 and 5 Mg ha-1 around white spruce seedlings planted on disc-trenched microsites. Soil solutions from lysimeters and soil samples were analyzed for exchangeable elements, soil pH and electrical conductivity while white spruce tissue samples were analyzed for various elements. Applications of pulp mill wood/sludge ash material significantly increased the soil pH from 4.8 to 6.9, resulting in increased soil extractable and soil solution P, Ca, and Mg. Soil electrical conductivity (EC) was increased from about 0.02 dS m-1 to approximately 0.10 dS m-1 by the heavier application rate of ash. In addition, at higher ash application rates, white spruce seedling growth was significantly decreased, suggesting salt phytotoxicity effects from the ash. These results indicate that with proper rate determination and management of salt effects, land application of this material would not appear to pose serious problems for white spruce seedling establishment. Key words: Picea glauca, wood/sludge ash, Luvisols, lysimeters, electrical conductivity, pH


1990 ◽  
Vol 30 (3) ◽  
pp. 421
Author(s):  
RL Aitken ◽  
RA Stephenson ◽  
EC Gallagher

Glasshouse experiments were undertaken to evaluate the effects of soil pH on macadamia (Macadamia integrifolia Maiden and Betche) seedlings and to examine seedling growth in relation to soil chemical properties in acidic soils. In one experiment, in which 13 rates of CaCO3 (0 to the equivalent of 12 000 kg/ha) were applied to a strongly acidic (pH 3.9, 1:5 in water) sandy loam, optimum seedling growth was obtained in the pH range 4.0-5.9. A second experiment, in which seedlings were grown in each of 3 strongly acidic soils amended with various rates of CaCO3, also showed that macadamia seedlings could grow satisfactorily at pH values of 4.0 (2 soils) and 4.5 (1 soil). Increased seedling growth on 2 soils (silty clay loam, experiment 1; sandy loam, experiment 2) treated with lime was due to amelioration of aluminium and/or manganese toxicity and not to the alleviation of calcium deficiency. The results indicate that soil pH measurement alone would not be a good indicator of seedling growth. In some soils, seedling growth was optimum at pH 3.9, whereas at pH 4.0 in another soil, growth was well below the maximum which was attained at pH 4.5. The significant (P<0.05) growth reductions that occurred on all soils limed to pH values >6.0 were attributed to induced micronutrient deficiencies.


Author(s):  
Ziwei Xiao ◽  
Xuehui Bai ◽  
Mingzhu Zhao ◽  
Kai Luo ◽  
Hua Zhou ◽  
...  

Abstract Shaded coffee systems can mitigate climate change by fixation of atmospheric carbon dioxide (CO2) in soil. Understanding soil organic carbon (SOC) storage and the factors influencing SOC in coffee plantations are necessary for the development of sound land management practices to prevent land degradation and minimize SOC losses. This study was conducted in the main coffee-growing regions of Yunnan; SOC concentrations and storage of shaded and unshaded coffee systems were assessed in the top 40 cm of soil. Relationships between SOC concentration and factors affecting SOC were analysed using multiple linear regression based on the forward and backward stepwise regression method. Factors analysed were soil bulk density (ρb), soil pH, total nitrogen of soil (N), mean annual temperature (MAT), mean annual moisture (MAM), mean annual precipitation (MAP) and elevations (E). Akaike's information criterion (AIC), coefficient of determination (R2), root mean square error (RMSE) and residual sum of squares (RSS) were used to describe the accuracy of multiple linear regression models. Results showed that mean SOC concentration and storage decreased significantly with depth under unshaded coffee systems. Mean SOC concentration and storage were higher in shaded than unshaded coffee systems at 20–40 cm depth. The correlations between SOC concentration and ρb, pH and N were significant. Evidence from the multiple linear regression model showed that soil bulk density (ρb), soil pH, total nitrogen of soil (N) and climatic variables had the greatest impact on soil carbon storage in the coffee system.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Sanjutha Shanmugam ◽  
Sasha N. Jenkins ◽  
Bede S. Mickan ◽  
Noraini Md Jaafar ◽  
Falko Mathes ◽  
...  

AbstractCo-application of biochar and biosolids to soil has potential to mitigate N leaching due to physical and chemical properties of biochar. Changes in N cycling pathways in soil induced by co-application of biological amendments could further mitigate N loss, but this is largely unexplored. The aim of this study was to determine whether co-application of a biochar and a modified biosolids product to three pasture soils differing in texture could alter the relative abundance of N cycling genes in soil sown with subterranean clover. The biosolids product contained lime and clay and increased subterranean clover shoot biomass in parallel with increases in soil pH and soil nitrate. Its co-application with biochar similarly increased plant growth and soil pH with a marked reduction in nitrate in two coarse textured soils but not in a clayey soil. While application of the biosolids product altered in silico predicted N cycling functional genes, there was no additional change when applied to soil in combination with biochar. This supports the conclusion that co-application of the biochar and biosolids product used here has potential to mitigate loss of N in coarse textured soils due to N adsoption by the biochar and independently of microbial N pathways.


Soil Research ◽  
1989 ◽  
Vol 27 (1) ◽  
pp. 45 ◽  
Author(s):  
IR Willett

In a laboratory experiment, samples of 18 soils, which are known to be flooded in the field, were flooded for up to 32 days. Both untreated and phosphate-treated (50 mg P kg-1) soils were studied. It was attempted to identify which chemical properties measured on the dry untreated soils, and the changes in pH, Eh and extractable Fe and Mn over the flooding periods, controlled the changes in sodium acetate buffer (pH 3.0) extractable phosphorus during flooding. It was shown that the reduction of iron(III) oxides was the dominant source of the P released during flooding. However, the amount of P released was strongly inhibited by re-sorption. Direct measurement of the amount of iron(III) reduced during flooding and measurement of phosphate sorption were required to predict the amount of P released during flooding. Organic matter contributed toward the P released during flooding. Its contribution appeared to be by mineralization, rather than by accelerating FeIII reduction. The reduction of MnIII and MnIII was a minor source of P in the untreated soils. Changes in soil pH during flooding were responsible for desorption of freshly applied P, but did not appear to affect P release in the untreated soils. The Vertisols and some of the Alfisols showed very little, or no release of P during flooding.


2005 ◽  
Vol 71 (12) ◽  
pp. 8714-8720 ◽  
Author(s):  
Belinda C. Ferrari ◽  
Svend J. Binnerup ◽  
Michael Gillings

ABSTRACT Traditional microbiological methods of cultivation recover less than 1% of the total bacterial species, and the culturable portion of bacteria is not representative of the total phylogenetic diversity. Classical cultivation strategies are now known to supply excessive nutrients to a system and therefore select for fast-growing bacteria that are capable of colony or biofilm formation. New approaches to the cultivation of bacteria which rely on growth in dilute nutrient media or simulated environments are beginning to address this problem of selection. Here we describe a novel microcultivation method for soil bacteria that mimics natural conditions. Our soil slurry membrane system combines a polycarbonate membrane as a growth support and soil extract as the substrate. The result is abundant growth of uncharacterized bacteria as microcolonies. By combining microcultivation with fluorescent in situ hybridization, previously “unculturable” organisms belonging to cultivated and noncultivated divisions, including candidate division TM7, can be identified by fluorescence microscopy. Successful growth of soil bacteria as microcolonies confirmed that the missing culturable majority may have a growth strategy that is not observed when traditional cultivation indicators are used.


2016 ◽  
Author(s):  
Abdulaha-Al Baquy ◽  
Jiu-Yu Li ◽  
Chen-Yang Xu ◽  
Khalid Mehmood ◽  
Ren-Kou Xu

Abstract. Soil acidity has become a serious constraint in dry land crop production systems of acidic Ultisols in tropical and subtropical regions of southern China, where winter wheat and canola are cultivated as important rotational crops. Regardless of other common existing concerns in acidic Ultisols of southern China, it needs to be investigated whether soil acidity has any effect on wheat and canola growth. There is little information on the determination of critical soil pH as well as aluminium (Al) concentration for wheat and canola crops. The objective of this study was to determine the critical soil pH and exchangeable aluminium concentration (AlKCl) for wheat and canola production. Two pot cultures with two Ultisols from Hunan and Anhui were conducted for wheat and canola crops in a controlled growth chamber, with a completely randomized design. A soil pH gradient ranging from 3.7 (Hunan) and 3.97 (Anhui) to 6.5, with three replications, was used as a treatment. Aluminium sulfate (Al2(SO4)3) and hydrated lime (Ca(OH)2) were used to obtain the target soil pH levels. Plant height, shoot dry weight, root dry weight, and chlorophyll content (SPAD value) of wheat and canola were adversely affected by soil acidity in both locations. The critical soil pH and AlKCl of the Ultisol from Hunan for wheat were 5.29 and 0.56 cmol kg−1, respectively. At Anhui, the threshold soil pH and AlKCl for wheat were 4.66 and 2.36 cmol kg−1, respectively. On the other hand, the critical soil pH for canola was 5.65 and 4.87 for the Ultisols from Hunan and Anhui, respectively. The critical soil exchangeable Al for canola cannot be determined from the experiment of this study. The results suggested that the critical soil pH and AlKCl varied between different locations for the same variety of crop, due to the different soil types and their other soil chemical properties. The critical soil pH for canola was higher than that for wheat for both Ultisols, thus canola was more sensitive to soil acidity. Therefore, we recommend that liming should be undertaken to increase soil pH if it falls below these critical soil pH levels for wheat and canola production.


2020 ◽  
Author(s):  
Chunhui Ma ◽  
Jiangjiao Qi ◽  
Xue Yu ◽  
Lihe Su ◽  
Tingting He ◽  
...  

Abstract Alfalfa (Medicago sativa L.) is an important forage legume in farming and animal husbandry systems. In this study, MiSeq high-throughput sequencing was applied to assess the relationship between bacterial and fungal community structures and alfalfa growth characteristics and soil physical and chemical properties induced by different cultivars alfalfa (Victoria, Kangsai, Aohan) in the grey desert soil. The results showed that the diversity of bacterial and fungal in Victoria was higher, and the bacterial diversity was significantly lower for alfalfa with Aohan than for the others, and the fungal diversity was lower for alfalfa with Kangsai than for the others. Heatmap showed that total nitrogen, fresh weight, pH and organic have significantly affect fungal community structure, whereas pH and organic carbon also significant effects on bacterial community structure. LefSe analysis showed that the growth adaptability of introduced alfalfa is mainly related to fungal and bacterial species, and the beneficial microorganisms with significant differences and relative high abundance are significantly enriched in Victoria. Pathogens with high relative abundance are mainly concentrated in Aohan alfalfa soil. Based on our findings, Victoria is the high-yield alfalfa suitable for planting in gray desert soil, while planting Kangsai and Aohan alfalfa needs probiotic for adjuvant.


Author(s):  
C. V. Ogbenna ◽  
V. E. Osodeke

Aim: A pot experiment was carried out to determine the effect of sawdust ash and lime (Ca(OH)2) on soil characteristics and yield of sunflower in acidic soil of southeastern Nigeria. Study Design: The experiment was laid out in split-plot design, using sawdust ash (0, 1, 2, 3, 4 t ha-1) as the sub plot and lime (0, 0.5, 1.0, 1.5 t ha-1) as the main plot. Place and Duration of Study: Study was conducted outdoors at Michael Okpara University of Agriculture Umudike, Nigeria, during the 2010 planting season. Materials and Methods: Treatment combinations were applied to the 60 buckets containing soil, mixed thoroughly and watered adequately. After 1 week of treatment application, two sunflower seeds were planted and later thinned to one seedling per bucket. Plant growth and yield data were collected. Pre planting and post-harvest soil samples were collected and analyzed for soil properties. Results: Results showed that with the exception of organic carbon there was significant effect of treatments on all soil chemical properties. Lime and sawdust ash (SDA) as single and combined treatments significantly increased total nitrogen (P=0.05), available phosphorus (P<0.010), and base saturation (P<0.012). The interaction between SDA and lime significantly (P=0.05) increased total exchangeable bases and effective cation exchange capacity, while soil pH was significantly increased (P=0.05) by single applications. The increases in soil chemical properties led to significant positive response of the sunflower. With the exception of number of leaves, other plant parameters (Plant height, stem diameter, head weight, 50 seed weight, head diameter) had significant increases for sawdust ash alone at P=0.05. Correlation studies showed positive significant relationship between soil pH and sunflower yield. Conclusion: The study showed that sunflower performed best at the combination of 3 tha-1 SDA and 1.5 t ha-1 lime producing a mean head weight of 45.4 g.


2019 ◽  
Vol 9 (2) ◽  
pp. 178
Author(s):  
I GUSTI PUTU RATNA ADI ◽  
I NYOMAN PUJA

Increased Rice Crop Productivity Through Compost and NPK Fertilization. The aims of this research was to study response of compost and NPK fertilizers to the soil chemical properties and rice yield. The method used was a factorial Randomized Block Design consisting of two factors. The first factor was compost Fertilizer (B) consists of 2 levels, namely: B0 = Without compost fertilizer and B1 = 5 ton compost fertilizer ha-1. The second factors was NPK national recomendation (P) consists of 3 levels, namely: P1 = 50% of NPK recommendation, P2 = 100% of NPK recommendation and P3 = 150% of NPK recommendation. The results showed that the treatment of compost and NPK fertilizers gave no significant effect on tillers number clumb-1 and dry straw weight/m2 , but gave a significant effect on N, P, K and dry grain weight/m2. The combination of 5 tons of compost/ha and 150% of NPK recommendation can produce N-total, P-available level, K-available and dry grain weight per m2 respectively 0.35%, 13.79 ppm, 355, 21 ppm and 0.96 kg and significantly higher than the combination of 50% NPK recommendation and without compost, which were 0.26%, 8.21 ppm, 236.10 ppm and 0.69 kg respectively.


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