scholarly journals The Effect of Using New Synbiotics on the Turkey Performance, the Intestinal Microbiota and the Fecal Enzymes Activity in Turkeys Fed Ochratoxin A Contaminated Feed

Toxins ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 578 ◽  
Author(s):  
Katarzyna Śliżewska ◽  
Paulina Markowiak-Kopeć ◽  
Anna Sip ◽  
Krzysztof Lipiński ◽  
Magdalena Mazur-Kuśnirek

The feed supplementation of probiotic microorganisms is a promising method for detoxification of ochratoxin A (OTA) in poultry. The aim of the study was to investigate the effect of newly elaborated synbiotics on the turkey performance, the intestinal microbiota and its enzymatic activity in turkeys (0–15 weeks) fed OTA contaminated feed (198.6–462.0 µg/kg) compared to control group (OTA-free feed). The studies determined the composition of intestinal microorganisms by the culture method and the activity of fecal enzymes by spectrophotometry. It was found that OTA had an adverse effect on the body weight, the intestinal microbiota and the fecal enzymes activity in turkeys. On the other hand, synbiotics resulted in an increase in the count of beneficial bacteria while reducing the number of potential pathogens in the digestive tract. Moreover, synbiotics caused an increase in the activity of α-glucosidase and α-galactosidase, while decreasing the activity of potentially harmful fecal enzymes (β-glucosidase, β-galactosidase, β-glucuronidase) in the turkey’s excreta. Results indicate a beneficial effect of elaborated synbiotics on the health of turkeys and a reduction of the negative impact of OTA contaminated feed. These synbiotics can be successfully used as feed additives for turkeys.

Author(s):  
M. Grigoryev ◽  
N. Chernogradskaya

Animals imported to Yakutia from other regions fall into feeding and housing conditions that differ from their usual agro-climatic conditions, which causes the problem of their adaptation. Yakutia is one of the areas where the feed of its own production has a lack of basic minerals, which affects the health and productivity of animals, which has an additional negative influence on the adaptation process of imported cattle. In order to replenish macro- and microelements in the body, it is necessary to introduce various local mineral feed additives, premixes, and use mineral salts into the ration. Under the conditions of Central Yakutia the influence of local mineral feed additives (zeolite of the Khonguruu Deposit in the Suntar district and sapropels of local lakes) containing macro- and microelements that increase the meat productivity of steers has been studied. As a result of the research the influence of local mineral feed additives on meat productivity and physiological condition of Hereford steers has been established. Influence of zeolite and sapropel in a complex with mineral salts on digestibility of nutrients of the ration and use of nitrogen, calcium and phosphorus by experimental animals has been defined. The influence of feed additives on the quality of meat products has been studied. The results have shown that the use of local mineral feed additives in the composition of ration of steers during the fattening period at a dose of 0,7 g zeolite per 1 kg of live weight of the animal and 200 g of sapropel in combination with 10 g of mineral salts contributed to the gain in live weight, carcass weight, carcass yield, yield of edible parts, and economic efficiency of fattening. For the entire period of the experiment the gain in live weight in groups was: in control group 2222,9 kg, in the 1st experimental group 2396,2 kg, in the 2nd experimental group 2471,1 kg. The total economic effect for the 1st experimental group for the period of experiment amounted to 44,3 thousand Rubles, for the 2nd experimental group 78,0 thousand Rubles or per 1 head/day 14,06 and 24,76 Rubles, respectively.


Author(s):  
S. Grikshas ◽  
N. Kulmakova ◽  
K. Spitsyna ◽  
A. Dar’in ◽  
T. Mittelshtein

Mycotoxins have been formed in feed are secondary metabolites of fungi and are quite stable substances that have teratogenic, mutagenic and carcinogenic effects. An effective way to combat mycotoxins in feed is the use of feed additives that adsorb toxins, prevent their absorption in the gastrointestinal tract of the animal and are excreted from the body. The infl uence of coconut enterosorbent Shelltic Es on fattening and meat qualities of young pigs has been studied. It has been found in the process of fattening that the highest feed digestibility was in pigs of the experimental group, in the diet of which enterosorbent has been added. The results of studies of the chemical composition and technological properties of pork have been provided. The positive eff ect of enterosorbent on precocity, absolute average daily gain of live weight and reduction of feed expenditures per 1 kg of gain has been revealed. In pigs from the experimental group the average thickness of the fat was higher and the area of the “muscle eye” was lower compared with animals of the control group, which indicates that higher rates of carcass yield have been obtained due to faster accumulation of fat tissue. The weight of internal organs of pigs indicates the intensity of metabolic processes in the body. In experimental animals the weight of the lungs was 0,1 kg lower, and the liver and heart were higher by 0,13 and 0,01 kg, respectively. Enterosorbent had no effect on the content of vitamins in the liver of pigs of the compared groups. The content of impurities of organochlorine toxicants and toxic elements in the meat and liver of animals of the experimental group was lower than that of control analogues. Therefore, the use of enterosorbent Shelltic Es promotes more active excretion of them from the body.


2022 ◽  
Author(s):  
Makaeva Ayna Maratovna ◽  
Sizova Elena Anatolevna ◽  
Atlanderova Ksenya Nikolaevna

Determining which forms of mineral feed additives can increase farm animal productivity is a key area of research. This study assessed the mineral composition of ruminal fluid and the effectiveness of calcium and phosphorus used by animals after the introduction of finely dispersed particles (FDP) of SiO2 (group I) and FeCo (group II) with a hydrodynamic radius of 109.6 ± 16.6 and 265 ± 25 nm, respectively. The deposition and use of calcium and phosphorus in the body of the experimental animals exceeded the control values. In group I, 30.8% more calcium was deposited (p ≥ 0.05), and in group II, the value was 30.3% (p ≥ 0.01). In the experimental groups, the calcium utilization rate was 27.3% higher in group I (p ≥ 0.05), and 28.2% higher in group II (p ≥ 0.01) compared to in the control. Phosphorus deposition was 34% higher (p ≤ 0.01) in experimental group I and 6% higher in experimental group II, compared with the control. Group I had a high utilization rate of phosphorus from the feed (with a 29% difference compared to the control). Comparison of the experimental groups revealed that the introduction of SiO2 FDP promoted an increase in the concentration of silicon, phosphorus, and calcium in the ruminal fluid. The introduction of FeCo FDP was accompanied by a decrease in the concentration of iron and cobalt in the ruminal fluid. Thus, the use of feed additive in finely dispersed form in the diet of animals was accompanied by an increase in the use of calcium and phosphorus by the animal’s body, which is advisable when intensifying milk and meat productivity. The obtained results require further research. Keywords: finely dispersed forms of microelements, ruminants, calcium and phosphorus exchange, feeding


2021 ◽  
Vol 6 (2) ◽  
pp. 227-235
Author(s):  
O. Litovchenko ◽  
◽  
I. Perova ◽  

In modern conditions there is a high need to improve methods for establishing the nature of the combined influence of factors using modern approaches for further development of prevention measures against the negative impact of factors on the body. The purpose of the study was to investigate the combined effect of electromagnetic radiation and low temperatures with subsequent determination of the level of contribution of each factor in the formation of biological effects within the experiment by mathematical analysis of data using the method of artificial intelligence. Materials and methods. The subchronic experiment (30 days) was performed on male rats. The animals were divided into groups: combined exposure to electromagnetic radiation (70 kHz, 600 V/m) and reduced temperature (40C), isolated exposure to reduced temperature, isolated exposure to electromagnetic radiation and a control group. The establishment of biological effects was performed at stages 5, 15 and 30 days of the experiment. Changes in the body were assessed by physiological, biochemical and immunological parameters of the blood (30 indicators). Data processing was performed using computational intelligence methods (Neuro-fuzzy system). Results and discussion. The combined effect of the factors was manifested by the following biological effects: intensification of peroxidation processes with simultaneous moderate suppression of antioxidant protection, intensification of lipid metabolism was characterized by dyslipoproteinemia, manifesting in: increase in triglycerides, cholesterol and its fractions. Changes of the immune system were decrease in the ability of neutrophils to phagocytosis at different stages of the experiment, imbalance of the activation of the system of reducing the concentration of C4 at the background of increased intensity of IgM and IgG synthesis. The method of determining the intensity of the factors, developed on the basis of factor and cluster analysis, allowed to determine that during the experiment the ratios between the factors changed, but the greatest contribution was made by reduced temperature by both biochemical parameters (63%) and immunological (53%) at the same time, the level of electromagnetic radiation contribution was 37% and 47%, respectively. Thus, the reduced temperature was the leading factor in the combined effect, which added 60% to the overall biological effect throughout the study, against the effect of electromagnetic radiation for which the level of contribution was only 40%. By means of the factor analysis informative indicators, making it possible to establish the characteristics of biological effects, and therefore leading indicators in formation of biological effects were the increase in concentration of DC with simultaneous decrease in concentration of SH-groups, increase in VLDL and suppression of oxygen-dependent metabolism of neutrophil-test. Conclusion. Thus, the tested mathematical approach allowed to determine the leading role of each of the studied factors under the conditions of their combined influence in different systems, which allows to predict probable appropriate reactions of the whole organism and gives a reasonable approach to the development of preventive measures


Author(s):  
L. P. Yarmots ◽  
G. A. Yarmots ◽  
A. E. Belenkaya ◽  
M. O. Smyshlyaeva

Unbalanced mineral and vitamin nutrition of lactating cows can be a critical factor in the realization of their productive potential. The development and introduction into production of inexpensive, but effective feed additives is the main trend of improving the feeding of modern animal husbandry. Under the conditions of the Tyumen region such natural raw materials can be sapropels, which successfully combine a variety of biologically active substances. The purpose of the research was to study the digestibility of nutrients and energy metabolism in lactating cows when sapropel is included in the ration. Scientifi c and economic experiment in the study of the effectiveness of feeding sapropel lake Nepryak has been carried out in the training and experimental farm of the State Northern Trans-Urals Agrarian University. The animals of the control group have been fed the main diet. Cows of the 1st and 2nd experimental groups have been fed 300 and 500 g of sapropel per head/day, respectively, in addition to the main ration. It has been found as a result of research that the introduction of sapropel into the ration of cows has a positive effect on the digestibility of nutrients. The digestibility coefficients were higher in the animals of the 1st and 2nd experimental groups compared with the cows of the control group. Cows from the experimental groups digested all the organic substances of the ration better. During the experiment period cows of the 1st and 2nd experimental groups in contrast to the control group had higher milk yield by 11,31 and 10,38 %, respectively, milk protein yield by 10,56 and 5,95 %, milk fat yield by 17,18 and 12,99 %. It has been established that the introduction of sapropel into the ration of cows has the positive effect on the digestibility of nutrients, contributes to the increase of milk productivity, normalizes the metabolism in the body.


2020 ◽  
Vol 10 (5) ◽  
pp. 278-286
Author(s):  
M.D. Kambur ◽  
A.Y. Lermontov ◽  
A.Y. Lermontov

Changes in the secretory activity of mammary gland during the involution period are designed to create conditions for inexhaustible use of the mammary gland, ensure the normal course of structural and functional regression for the development for the next lactation. For animals in which the interlactation period lasted less than 45 days, the absorption of non-esterified fatty acids in the last week of the involution period was 2.8 times higher than in animals in which the interlactation period lasted at least 55 days, 1.48 times higher than the absorption of acetic acid, 1.24 times higher than β-oxybutyric acid, 1.28 times higher than glucose, 2.82 times the total amount of phospholipids and triacylglycerols. For acetic acid, β-oxybutyric acid and glucose, not only higher absorption rates were observed, but also a tendency to increase the absorption level in the last weeks of lactation in animals with an interlactation period of less than 45 days, indicating metabolic changes in mammary gland tissue during the involution period. The duration of the dry period affects the secretory activity of breast tissue during lactation and the quality of milk produced during this period, which is expressed by lower fat content in the product while reducing the duration of the dry period to less than 45 days and reducing milk fat by 10.42% during the next calving. At the same time, the body weight of newborn calves in animals, whose interlactation period was less than 45 days by 19.3%, was lower than the animals of the control group, i.e., those who were in the interlactation period for at least 55 days, which indicates the negative impact of reducing the duration of the dry period on the body of animals.


2020 ◽  
Vol 48 (2) ◽  
pp. 116-126
Author(s):  
R Islam ◽  
MM Hossain ◽  
F Nargis ◽  
ME Hossain

This study was conducted to observe the effect of different administration method of garlic and neem on growth performances, feed efficiency and serum biochemical parameters for safe broiler production. The experiment was done for a period of 32 days with 384 one-day-old straight run broiler chicks. The broiler chicks were divided into six groups each of 64 birds and replicated to four subgroups each of 16 birds. The dietary groups were; control (basal diet; no additives), antibiotic (basal diet + antibiotic), garlic in feed (basal diet + 0.25% garlic powder), garlic in water (basal diet + garlic extracts), neem in feed (basal diet + 0.25% neem powder) and neem in water (basal diet + neem extracts). Results showed that the body weight and body weight gain increased significantly (P<0.05) in both the garlic and neem groups compared to the control group. Feed intake was not different (P>0.05) among the treatment groups. Garlic in feed and water and neem in feed groups showed better FCR (P<0.05) compared to the other treatment groups. There were no significant (P>0.05) difference in meat yield, bone development and dressing parameters except head and gizzard in different dietary groups. Supplementation of garlic with feed and water significantly (P<0.05) decrease cholesterol, triglyceride and LDL compared to the other groups. However, both the garlic and neem groups showed numerically higher HDL compare to the control group. There were no 0significant (P>0.05) differences in serum glucose and GPT among different treatment groups. GOT was significantly (P<0.05) lower in neem groups than that of garlic and antibiotic groups. Additive groups showed higher profitability than control group. Based on the results of the study, it may be suggested that the garlic and neem could be used both in feed and water as potential feed additives for safe broiler production. Bang. J. Anim. Sci. 2019. 48 (2): 116-126


Author(s):  
T. A. Holubieva

The expediency of use of brewer’s dried grain in feeding of the young quails meat direction of productivity. It has no negative impact on digestibility and balance of Nitrogen in the body. The study was conducted in a scientific and experimental laboratory of feed additives of the National University of Life and Environmental Sciences of Ukraine on quail breed Pharaoh. Four groups of quails were formed with 100 heads each. Physiological experiments were carried out at 16–21 and 30–35 days old. For the experiments from the experimental groups, 4 heads were selected. As a result, it was proved that the digestibility of the feed changed with age and did not depend on the level of input of the brewer’s dried grain. Adding 2–4% of brewer’s dried grain in the mixed fodder contributed to the increase in organic matter digestibility by 1.5% and 1.7% in the first period and 2–2.6% in the second period of experience. In physiological experiments, the digestibility of the fiber was lower in the poultry, to which a brewer’s dried grain was added to the mixed fodder. The lowest digestibility of fiber was a bird with a level of 6% dry spent grain. Protein digestibility in poultry, which in the mixed fodder was introduced brewer’s dried grain, was higher than that which is not fed to a brewer’s dried grain.When reaching quails of 30–35-day-old age, the level of Nitrogen consumption in comparison with the previous period increased by 13–15%. The amount of Nitrogen consumed with food in the birds of the individual groups was different. The greatest amount of Nitrogen was retained in the body by quail, consuming mixed feeds containing 4% of dry beer pellets. They were controlling by 6.2% in this indicator. Compared with the control, less Nitrogen was contained in the body of the bird, which fed mixed fodder with 6% of dry beer pellets – by 1.2%.


Author(s):  
A. V. Mitfakhutdinov ◽  
E. R. Saifulmuliukov

Scientific publications describe the positive effect of feed additives and pharmacological complexes on metabolic processes in the body of broiler chickens, in particular, on the protein composition of the blood, meat productivity and protein accumulation in meat. The Peak-Antistress feed additive developed at the Department of Morphology, Physiology and Pharmacology of the South Ural State Agrarian University allows to reduce the technological load on the poultry body by stimulating the general metabolism and complex antioxidant action. Experiments on the use of the feed additive Peak-Antistress were carried out on broiler chickens of the final hybrid of the Arbor Acres cross in an industrial-type poultry farm with floor technology. The poultry was divided into three groups of 6,000 chickens each and kept in one workshop in separate sections. The control group received the main diet, the 1st experimental group received the main diet and feed additive at a dose of 1269 g / t of feed 5 days before slaughter, the 2nd experimental group received the main diet and feed additive at a dose of 1693 g / t of feed 5 days before slaughter. Slaughter of broiler chickens was carried out according to the technological instructions of the enterprise on the 38th day. Against the background of the use of the feed additive in the experimental groups of broiler chickens, stimulation of protein metabolism was observed, as evidenced by an increase in total protein in the blood by 7.9–20.1%, an increase in the level of protein in white meat by 0.1–0.3%, and a tendency to an increase in amino acids in white meat protein by 0.2–12.3%.


2018 ◽  
Vol 14 (2) ◽  
pp. 109-114
Author(s):  
Ольга Садыкова ◽  
Ol'ga Sadykova ◽  
Сергей Жолудев ◽  
Sergey Zholudev ◽  
Антон Еликов ◽  
...  

Subject. Removable dentures have a negative impact on supporting tissues, biochemical parameters and composition of the microflora of the oral cavity, secretory activity of the salivary glands and other processes of the maxillofacial region and the body as a whole. One of the methods that can be used in the treatment and prevention of prosthetic stomatitis is balneotherapy. In the article, using the clinical, biochemical, microbiological and histological methods, the effectiveness of the use of mineral water "Fateevskaya" in patients using removable orthopedic structures was studied. Objectives of the study was to evaluate the effectiveness of procedures for small balneotherapy with the use of mineral water "Fateevskaya" in patients with removable dentures. Methods.We examined 158 elderly and senile age patients with removable dentures. Correction of removable dentures was carried out to all patients at the initial stage. All examined patients are divided into two groups. The group received the treatment with the low balneotherapy with “Fateevskaya” mineral water (78 patients, and the clinical control group (80 patients). The clinical condition of oral cavity was assessed before and after the course of low balneotherapy. Were researched: changes of mucous membrane of prosthetic bed, salivation rates, disturbance of salivary secretion, oral microbiocenosis. Results.Of the research showed that in both groups were increase of pH value, appears lower values of total protein and glucose, decrease in activity of some enzymes. This explanation is due to a decrease in the stress response to using the removable dentures, normalization of salivary glands functioning, decrease in the intensity of the phlogiston process. The results of a histological research also confirm this. The results of microbiological crops before and after the low balneotherapy indicate the oral biocenosis normalization. The positive changes in the researched parameters had significantly larger statistically valid values in the group received the treatment with the low balneotherapy with “Fateevskaya” mineral water. The results of the complex research allow us to recommend the “Fateevskaya” mineral water to low balneotherapy in dental practice.


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