MEDIA ALTERNATIF PERTUMBUHAN Staphylococcus aureus DARI BIJI DURIAN (Durio Zibethinus murr)

Media that is often used for bacterial growth is Nutrient agar. The high price incurred for the manufacture of instant media becomes a constraint so that innovation is needed that can reduce the cost of expenditure that is looking for alternative media from materials that are easily available. One of the natural ingredients that have a source of nutrients that can be used for bacterial growth of durian seeds that have a high carbohydrate content as a source of carbon. The objective of this research was to know the growth of Staphylococcus aureus bacteria on alternative medium of durian seed as substitution media of Nutrient agar. This research is laboratory experimental research, the sample used is durian seed with three concentrations of 200 g, 300 g, 400 g. The parameters observed were calculating total plate number and morphological form of Staphylococcus aureus bacteria growth. The results showed that the highest population on medium durian medium was the concentration of 200 g as much as 5.7 x 10-4 cfu and the lowest population at the concentration of 400 g was 3.5 x 10-3 cfu. While the population on the media Nutrient agar that is 7.7 x 10-6 cfu. Based on morphological observation of bacterial growth on durian seed medium, round shape, size 0.1 cm, white milk color, smooth surface, and convex elevation. The conclusion of this research is the growth of Staphylococcus aureus on alternative medium of durian seeds can be used as substitution media of Nutrient agar.

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The Study of Bacillus Subtils Antimicrobial Activity on Some of the Pathological Isolates

International Journal of Drug Delivery Technology ◽

10.25258/ijddt.9.2.12 ◽

2019 ◽

Vol 9 (02) ◽

Author(s):

Hussein A Kadhum ◽

Thualfakar H Hasan2

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antimicrobial Activity ◽

Bacillus Subtilis ◽

Bacterial Growth ◽

Broad Spectrum ◽

Inhibitory Activity ◽

Bacterial Pathogens ◽

Inhibitory Ability ◽

Selection Of

The study involved the selection of two isolates from Bacillus subtilis to investigate their inhibitory activity against some bacterial pathogens. B sub-bacteria were found to have a broad spectrum against test bacteria such as Staphylococcus aureus and Pseudomonas aeruginosa. They were about 23-30 mm and less against Klebsiella sp. The sensitivity of some antibodies was tested on the test samples. The results showed that the inhibitory ability of bacterial growth in the test samples using B. subtilis extract was more effective than the antibiotics used.

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Penggunaan Limbah Logam Tembaga yang Didaur Ulang untuk Antibakteri dan Degradasi Metil Merah Secara Fotolisis

Jurnal Katalisator ◽

10.22216/jk.v4i1.3663 ◽

2019 ◽

Vol 4 (1) ◽

pp. 15

Author(s):

Ariyetti Ariyetti ◽

Muhammad Nasir ◽

Safni Safni ◽

Syukri Darajat

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Silver Nitrate ◽

Copper Sulfate ◽

Bacterial Growth ◽

Methyl Red ◽

Copper Metal ◽

Sulfate Hydrate ◽

Red Dye ◽

And Staphylococcus Aureus

Metil merah merupakan salah satu zat warna golongan azo yang sering digunakan dalam industri dan laboratorium. Penggunaan metil merah dapat menimbulkan efek terhadap kesehatan dan lingkungan. Oleh sebab itu dilakukan metode fotodegradasi dengan menggunakan semikonduktor dan radiasi sinar tampak. Semikonduktor yang digunakan yaitu berbahan dasar tembaga sulfat hidrat dan perak nitrat. Prekusor tembaga sulfat hidrat dibuat dari pengolahan limbah logam tembaga hasil pemotongan tembaga yang ada di bengkel Lembaga Ilmu Pengetahuan Indonesia (LIPI) Bandung. Bahan semikonduktor juga memiliki kemampuan dalam menghambat pertumbuhan bakteri. Hasil optimum yang didapatkan dalam proses fotodegradasi dan antibakteri merupakan gabungan antara kedua prekusor tembaga sulfat hidrat dan perak nitrat dengan bantuan penyinaran. Kemampuan dalam menghambat pertumbuhan bakteri didapatkan persentase kematian 100 % untuk masing-masing bakteri, yaitu Escherichia coli dan Staphylococcus aureus. Aktifitas fotokatalitiknya dengan konsentrasi semikonduktor 10 ppm untuk mendegradasi zat warna metil merah 5 ppm, selama 23 jam, dimana persentase degradasi yang didapatkan dengan penyinaran lebih tinggi dibandingkan dengan tanpa penyinaran. Pengaruh pH larutan terhadap degradasi metil merah yaitu optimum pada pH 12 (basa). Methyl red is one of the azo group dyes that is often used in industry and laboratories. The use of methyl red can have an effect on health and the environment. Therefore photodegradation method is done by using semiconductor and visible light radiation. The semiconductor used is based on copper sulfate hydrate and silver nitrate. The copper sulphate hydrate precursor is made from the processing of copper-cut copper metal waste in the workshop of the Indonesian Institute of Sciences (LIPI) in Bandung. Semiconductor materials also have the ability to inhibit bacterial growth. The optimum results obtained in the photodegradation and antibacterial process are a combination of both copper sulfate hydrate precursor and silver nitrate with the help of irradiation. The ability to inhibit bacterial growth obtained 100% mortality for each bacterium, namely Escherichia coli and Staphylococcus aureus. Photocatalytic activity with 10 ppm semiconductor concentration to degrade methyl red dye 5 ppm, for 23 hours, where the percentage of degradation obtained by irradiation is higher than without irradiation. The effect of pH of the solution on the degradation of methyl red is optimum at pH 12 (base).

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Fructose Enhanced Reduction of Bacterial Growth on Nanorough Surfaces

MRS Proceedings ◽

10.1557/opl.2013.35 ◽

2013 ◽

Vol 1498 ◽

pp. 73-78 ◽

Cited By ~ 2

Author(s):

N. Gozde Durmus ◽

Erik N. Taylor ◽

Kim M. Kummer ◽

Thomas J. Webster

Keyword(s):

Bacterial Growth ◽

Biofilm Formation ◽

Antibacterial Agents ◽

Bacteria Growth ◽

Planktonic Bacteria ◽

Combined Use ◽

Wide Range ◽

Metabolic Stimulation ◽

Antibacterial Surfaces ◽

First Time

ABSTRACTBiofilms are a major source of medical device-associated infections, due to their persistent growth and antibiotic resistance. Recent studies have shown that engineering surface nanoroughness has great potential to create antibacterial surfaces. In addition, stimulation of bacterial metabolism increases the efficacy of antibacterial agents to eradicate biofilms. In this study, we combined the antibacterial effects of nanorough topographies with metabolic stimulation (i.e., fructose metabolites) to further decrease bacterial growth on polyvinyl chloride (PVC) surfaces, without using antibiotics. We showed for the first time that the presence of fructose on nanorough PVC surfaces decreased planktonic bacteria growth and biofilm formation after 24 hours. Most importantly, a 60% decrease was observed on nanorough PVC surfaces soaked in a 10 mM fructose solution compared to conventional PVC surfaces. In this manner, this study demonstrated that bacteria growth can be significantly decreased through the combined use of fructose and nanorough surfaces and thus should be further studied for a wide range of antibacterial applications.

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Evaluation of Bacteriological Profile in Active Mucosal Chronic Otitis Media - A Cross-Sectional Study

Journal of Evidence Based Medicine and Healthcare ◽

10.18410/jebmh/2021/16 ◽

2021 ◽

Vol 8 (02) ◽

pp. 80-84

Author(s):

Smitha B ◽

Swapna U.P ◽

Salilkumar K

Keyword(s):

Staphylococcus Aureus ◽

Otitis Media ◽

Bacterial Growth ◽

Chronic Otitis Media ◽

Antibiotic Sensitivity ◽

Maximum Sensitivity ◽

Biochemical Tests ◽

Coagulase Negative Staphylococci ◽

Sensitivity Pattern ◽

Two Samples

BACKGROUND Active mucosal chronic otitis media (COM) is a disease of the middle ear cleft associated with inflammation and production of pus. The incidence is high in developing countries. It affects all age groups and both genders. If not properly treated, it can lead to complications. The bacterial isolates and their sensitivity pattern vary from place to place and also over time. Early identification of the microorganisms and their antibiotic sensitivity patterns helps in proper selection of antibiotics and quick recovery in COM patients. METHODS Fifty patients with active mucosal COM were selected for the study. The study was conducted over a period of one year. Ear swab from the deep external auditory canal was collected under strict aseptic precautions from these patients before starting antibiotic treatment. Microorganisms were identified by gram staining, growth on different agar plates, and various biochemical tests. Antibiotic sensitivity was done by the Kirby-Bauer method. RESULTS Majority of the patients were in the age group of 21 - 40 years (42 %) with female preponderance (62 %). All patients had a central perforation in the tympanic membrane (100 %). Bacterial growth was seen in 44 samples (88 %). Two samples (4 %) showed fungal growth. Four samples (8 %) showed no growth even after 48 hours of incubation. Single bacterium was isolated in 41 cases (82 %) whereas 3 samples (6 %) showed more than one bacterial growth. The predominant bacteria were Pseudomonas aeruginosa (50 %) followed by Staphylococcus aureus. The other isolates were coagulase negative staphylococci, enterococci, enterobacter, acinetobacter, and E coli. Pseudomonas showed maximum sensitivity to amikacin, imipenem and piperacillin / tazobactam (100 %). The most effective antibiotics for staphylococcus was vancomycin and linezolid. CONCLUSIONS The most common bacteria isolated in active mucosal COM were pseudomonas followed by Staphylococcus aureus. The most effective antibiotics for pseudomonas was amikacin, imipenem and piperacillin / tazobactam. Staphylococcus showed maximum sensitivity to vancomycin and linezolid. KEYWORDS Chronic Otitis Media (COM), Active Mucosal, Ear Swab, Bacteriology, Sensitivity

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Microbiological Stability of Rice Tart Stored at Ambient Temperature after Baking

Journal of Food Research ◽

10.5539/jfr.v6n5p41 ◽

2017 ◽

Vol 6 (5) ◽

pp. 41

Author(s):

Nguessan Elise ◽

Cisse Mohamed ◽

Niyonzima Eugene ◽

Sindic Marianne

Keyword(s):

Staphylococcus Aureus ◽

Storage Conditions ◽

Aerobic Bacteria ◽

Microbiological Analysis ◽

End User ◽

European Regulation ◽

Bacteria Growth ◽

Safety Standards ◽

Consumer Safety ◽

Microbiological Stability

Very popular in Belgium, rice tart is a hot pastry sold in bakeries. It is then consumed at home, for dessert or snack. This study is conducted to investigate the microbiological stability of this foodstuff, from the end of baking to end user by consumers. In this purpose, 108 rice tart samples were collected from each of seven bakeries in five Belgium provinces. Physico-chimical analysis in addition to microbiological analysis were carried out in accordance with the European Regulation EC 2073/2005 and with references methods, to enumerate the total microorganisms count, Staphylococcus aureus, Bacillus cereus as well as Enterobacteria, susceptible likely to contaminate the tarts during the production or after baking. Even when the results meet the microbiological safety standards immediately after baking in all bakeries implicated, a significant (p <0,05) increase of mesophilic aerobic bacteria (ranging from <1 to 7 log cfu/g) and B. cereus (> 3,7 log cfu/g) was observed during the storage at nonrefrigerated temperature (28 to 30°C). A post-baking recontamination and other parameters like an insufficient baking time or a non uniform distribution of the oven heat could explain the observed bacteria growth. The present study shows that most rice tarts investigated are microbiologically safe. However, the possible increase of bacteria load in this foodstuff attributable to the favorable aw, pH and temperature conditions, highlight the importance of applying good hygienic practices and compliance with storage conditions after baking to ensure consumer safety.

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ISOLASI BAKTERI YANG BERSIMBION DENGAN ASCIDIAN Herdmania momus YANG MEMILIKI AKTIVITAS ANTIBAKTERI

JURNAL PESISIR DAN LAUT TROPIS ◽

10.35800/jplt.8.2.2020.28766 ◽

2020 ◽

Vol 8 (2) ◽

pp. 20

Author(s):

Silvia E Koyongian ◽

Deiske A Sumilat ◽

Rosita A J Lintang ◽

Stenly Wullur ◽

Sandra O Tilaar ◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Marine Invertebrates ◽

Nutrient Agar ◽

Symbiotic Bacteria ◽

Bacterial Isolates ◽

Marine Broth ◽

Antibacterial Screening ◽

Coral Reef Ecosystems ◽

And Staphylococcus Aureus

Ascidian is marine invertebrates in coral reef ecosystems that produce many bioactive compounds for pharmacology. The presence of symbiotic bacteria with marine organisms is protected the host biota by producing secondary metabolites. The purpose of this study is to obtain symbiotic bacterial isolates with Herdmania momus ascidian, then to observe the antibacterial activity of these bacterial isolates against Escherichia coli, and Staphylococcus aureus. Isolation and culture of the symbiotic bacteria were made on Nutrient Agar and Zobell Marine Broth media. The antibacterial screening showed that the Herdmania momus symbiotic bacteria were able to inhibit the growth of Staphylococcus aureus and Escherichia coli.Keywords: ascidians, Herdmania momus, bacteria, isolation, antibacterialAbstak Ascidian adalah avetebrata laut di ekosistem terumbu karang yang banyak menghasilkan senyawa bioaktif untuk bidang farmakologi. Keberadaan bakteri yang bersimbion dengan organisme laut pada umumnya untuk melindungi biota yang ditumpanginya dan dirinya dengan cara menghasilkan senyawa metabolit sekunder. Tujuan dari penelitian ini yaitu untuk mendapatkan isolat bakteri yang bersimbion dengan ascidian Herdmania momus, kemudian mengamati aktivitas antibakteri dari isolat bakteri tersebut terhadap Escherichia coli, dan Staphylococcus aureus. Isolasi dan kultur bakteri yang bersimbion dengan ascidian dibuat pada media Nutrient Agar dan Zobell Marine Broth. Skrining aktivitas antibakteri menunjukkan isolat bakteri yang bersimbion dengan ascidian Herdmania momus mampu menghambat pertumbuhan organisme uji Staphylococcus aureus dan Escherichia coli.Kata kunci: ascidian, Herdmania momus, bakteri, isolasi, antibakteri

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Aktivitas Antibakteri Ekstrak Etanol Daun Akar Kaik-Kaik (Uncaria cordata (Lour.) Merr.) terhadap Staphylococcus aureus dan Salmonella typhi.

Jurnal Ilmiah Kesehatan ◽

10.33221/jikes.v19i03.598 ◽

2020 ◽

Vol 19 (03) ◽

pp. 107-114

Author(s):

Tiah Rachmatiah ◽

Vilya Syafriana ◽

Fitria Helma

Keyword(s):

Staphylococcus Aureus ◽

Salmonella Typhi ◽

Nutrient Agar

Akar kaik-kaik (Uncaria cordata (Lour.) Merr.) adalah tumbuhan yang daunnya mengandung senyawa aktif seperti tanin, saponin dan flavonoid. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak etanol daun akar kaik-kaik (Uncaria cordata (Lour.) Merr.) terhadap Staphylococcus aureus dan Salmonella typhi. Ekstrak dibuat secara maserasi serbuk daun akar kaik-kaik dalam etanol 96%. Uji aktivitas antibakteri dilakukan dengan metode difusi cakram pada media Nutrient Agar (NA) dan konsentrasi hambat minimum (KHM) ditentukan secara dilusi padat pada media NA. Hasil penelitian menunjukkan bahwa ekstrak etanol daun akar kaik-kaik mempunyai aktivitas antibakteri terhadap S. aureus pada konsentrasi 5%, 10%, 15%, 20%, 25%dan 30% dengan diameter daya hambat berturut-turut 8,46; 10,78; 12,55; 16,67; 17,69; dan 19,35; mm dan terhadap S. typhi pada kosentrasi yang sama memberikan diameter daya hambat 7,51; 7,83; 12,08; 14,00; 15,82; dan 16,24mm. KHM ekstrak etanol daun akar kaik-kaik terhadap kedua bakteri ada pada konsentrasi 4%.

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Homeopathy: a possible weapon against multidrug-resistant bacteria to antibiotics

International Journal of High Dilution Research - ISSN 1982-6206 ◽

10.51910/ijhdr.v13i47.727 ◽

2021 ◽

Vol 13 (47) ◽

pp. 114-114

Author(s):

TÃ¢nia Aguiar Passeti ◽

Ana Paula Macedo Souza ◽

Leandro Ribeiro Bissoli ◽

Registila Libania Beltrame ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Present Report ◽

Culture Liquid ◽

Biological Tissues ◽

Multidrug Resistant ◽

Resistant Bacteria ◽

Bacteria Growth ◽

Mueller Hinton ◽

Biochemical Mechanisms ◽

Bacteria Resistance

Background: The antimicrobial resistance is a genetic phenomenon, related to the existence of the gens restrained in microorganism that codify different biochemical mechanisms that obstruct the drugs actions. Some species present resistance widespread in all over the world, like the case of Staphylococcus aureus. This is one of the main bacteria that, in a period of time, has got multiple resistance against the antibiotics, and itâ€™s also, an important agent causative of the nosocomiais infections. The present report evaluated the action of the different homeopathic medicines about the growth of the bacteria Staphylococcus aureus and Staphylococcus aureus MRSA (Methicillin-resistant Staphylococcus aureus) â€œin vitroâ€. Methods: Doses of 150, 250 and 350 ÂµL of the homeopathic medicines Silicea, Hepar sulfor, Belladona, Arnica montana, Mercurio solubilis and nosode of Stafilococcus aureus, in the dynamism 6cH, 12cH e 30cH had been placed in 3mL culture liquid Mueller Hinton. It was added to this blend 10 ÂµL of a diluted bacterial solution 1/10, where of the solution in 0,5 of the Macfarley scale in 37Â°C, the growth in the tubes was evaluated in Spectrophotometric of 600 nm. Results: The results demonstrated that, for the Staphilococcus aureus, we have got significant bacteria inhibition in about 70 to 90% of the growth â€œin vitroâ€, provided by the homeopathic medicines Hepar sulfor in the dynamism of 30cH, Belladona in the dynamisms of 6cH and 30cH, in the Staphilococcus aureus nosode in the dynamisms 6cH and 30cH and Silicea in the dynamism of CH6, with regard to the control with alcohol 30%. The Staphilococcus aureus MRSA presented inhibition from 40% to 20% of the bacteria growth â€œin vitroâ€ related to the control with alcohol 30%, with the same medicines used before. Conclusion: We can conclude that the homeopathic medicines have an inhibitory action in the bacteria growth, including in bacteria resistance to the antibiotics. This information can suggest that a concerted action of antibiotics and homeopathic medicines, could improve the action of the antibiotics in the bacteria causative of infections in the biological tissues.

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