scholarly journals Evaluasi Ekstrak Tali Putri (Cassytha Filiformis Linn) Terhadap Efek Diuretik Dan Daya Larut Batu Ginjal

2018 ◽  
Vol 1 (2) ◽  
Author(s):  
Suci Ahda Novitri ◽  
Helmi Arifin Arifin ◽  
Rusdi Rusdi Rusdi
Keyword(s):  
Kidney Stones ◽  
Kidney Failure ◽  
Kidney Stone ◽  
Urine Volume ◽  
Positive Control ◽  
Negative Control ◽  
Diuretic Effect ◽  
Anova Analysis ◽  
Volume Factor

                                                ABSTRAK                                    Batu ginjal merupakan salah satu penyebab terjadinya gejala gagal ginjal kronik dan akut. Tali putri ini secara tradisional dipercaya mengobati batu saluran kencing. Penelitian ini bertujuan untuk mengetahui efek diuretik terhadap tikus jantan dan daya larut batu ginjal secara in vitro. Efek diuretik diuji dengan lima kelompok yang terdiri dari kontrol positif diberi furosemid, kontrol negatif diberi larutan NaCMC 5% dan tiga kelompok lagi diberi ekstrak tali putri dengan dosis 37,5mg/kgBB, 75 mg/kgBB dan 150 mg/kgBB secara berurutan. Tikus diberi perlakuan selama 15 hari dengan pengumpulan volume urin 2 jam dan 24 jam pada hari ke-5,10 dan 15. Hasil analisa ANOVA-two ways dengan Duncan’s Test menunjukkan adanya pengaruh volum urin 24 jam terhadap faktor perlakuan secara sangat bermakna (P<0,01) dan faktor waktu secara bermakna (P<0,05). Sedangkan hasil pengukuran volume urin 2 jam terhadap faktor perlakuan dan waktu berbeda secara sangat bermakna (P<0,01). Efek daya larut batu ginjal ekstrak tali putri meningkat dengan peningkatan konsentrasi.Kata kunci: Cassytha filiformis, diuretik, batu ginjal Kidney stones are one of the causes of symptoms of chronic and acute kidney failure. Cassytha filiformis is believed to be able to cure kidney stones. This study aims to determine the diuretic effect on rat and solubility of kidney stone in vitro. The diuretic effect with five groups consisting of positive control was given furosemide, negative control was given 5% NaCMC and three more groups with extracts of 37.5mg / kgBW, 75 mg / kgBW and 150 mg / kgBW respectively. Rats were treated for 15 days with a volume of urine 2 hours and 24 hours on days 5.10 and 15. The results of the two-way ANOVA analysis with the Duncan’s test to determine the 24-hour urine volume factor significantly (P <0, 01) and time factor significantly (P <0.05). While the results of measurements of urine volume 2 hours against factors and time were significantly different (P <0.01). The solubility of kidney stones to Cassytha filiformis’s extract increases by increasing it’s concentration.Keyword: Cassytha filiformis, diuretic, kidney stone 

ANALYSIS ON CALCIUM SOLUBILITY IN KIDNEY STONES (IN VITRO) AND DIURETIC EFFECT (IN VIVO) USING CORN SILK (ZEA MAYS L.) INFUSE

2018 ◽  
Vol 11 (13) ◽  
pp. 80
Author(s):  
Tuty Roida Pardede ◽  
Muchlisyam Muchlisyam
Keyword(s):  
Zea Mays ◽  
Kidney Stones ◽  
Kidney Stone ◽  
Zea Mays L ◽  
Urine Volume ◽  
Male Rats ◽  
Diuretic Effect ◽  
Corn Silk

Objective: Investigation has been carried out about infuse of corn silk (Zea mays L.) concerning the solubility of kidney stone in vitro and the diuretic effect in vivo. Methods: The methodology of research conducted in the form of dissolving kidney stones in corn silk infuse with a concentration of 2%, 4%, 6%, 8%, and 10%, then incubated at 37°C for 4 h. The solubility of calcium is known by measuring the levels of early calcium and after incubated with kidney stones. Potassium (K) is a substance that can dissolve kidney stones in infuse and also measured by atomic absorption spectrophotometry. The diuretic effect study used male rats which were divided into 7 groups that consisted of 1 positive control (treated with furosemide), 1 negative control treated with CMC Na 0.5 % and 5 groups treated with infuse of corn silk in 5 different concentration (2%, 4%, 6%, 8%, and 10%).Results: Increase in calcium levels after incubation with kidney stone using corn silk infuse with various concentrations. The levels of Ca dissolved in the infuse of 2%, 4%, 6%, 8%, and 10%, respectively, were 2.2600 μg/ml, 5.5733 μg/ml, 7.9267 μg/ml, 10.9233 μg/ml, and 8.7667 μg/ml. It was found that the urine volume for 24 h was significant with 2%, 8%, and 10% corn silk infuse.Conclusion: Infuse of corn silk could decrease kidney stones, and it was diuretic. This research supported the aim of traditional corn silk decoction for the same intention.

scholarly journals UJI AKTIVITAS DIURETIK EKSTRAK AIR TANAMAN SELEDRI (Apium graviolens L.) PADA TIKUS PUTIH JANTAN

2018 ◽  
Vol 10 (1) ◽  
pp. 43-46
Author(s):  
Adita Silvia Fitriana ◽  
Dina Febrina ◽  
Galih Samodra ◽  
Adita Silvia Fitriana ◽  
Dina Febrina ◽  
...  
Keyword(s):  
White Male ◽  
Urine Volume ◽  
Water Extract ◽  
Positive Control ◽  
Negative Control ◽  
Male Rat ◽  
Preparation Process ◽  
Diuretic Effect ◽  
Made In

Processing of celery as diuretic is done by society in various ways. So far, the most effective preparation process of celery extract has not been known. This research aims to examine the diuretic effect of various celery extract preparation.  This research was conducted using celery water extract which is made in accordance with the people’s habit, using aquades as a negative control and HCT as a positive control. The effectiveness test of celery water extract as diuretic was done on white male rat by measuring the urine volume every hour for six hours. The results showed that the largest urine volume is obtained from the boiling celery extract.

scholarly journals Aktivitas Kombinasi Ekstrak Etanol Daun Sirsak (Annona muricata L.) Dan Daun Pegagan (Centella asiatica L.Urb) Terhadap Kelarutan Kalsium Batu Ginjal Secara In Vitro

2017 ◽  
Vol 3 (1) ◽  
pp. 34-42
Author(s):  
Ni Wayan Swintari ◽  
Yuliet Yuliet ◽  
Khilda Khaerati
Keyword(s):  
Kidney Stones ◽  
Leaf Extract ◽  
Centella Asiatica ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Annona Muricata ◽  
Effective Combination ◽  
Dose Combination

Soursop (Annona muricata L.) and gotu kola (Centella asiatica L.Urb) is a plant that can be used as a laxative medicine kidney stones. This is because of the content of bioactive compounds including flavonoids, especially the leaf section. The use of a combination of soursop leaf extract and gotu kola can improve their effectiveness in order to remove calcium kidney stones. Therefore, this study aims to determine the activity and to determine dose combination soursop leaf ethanol extract (SS) and gotu kola leaf (GK) which is effective for dissolving potassium kidney stones. Extracts prepared by maceration method using ethanol 96% then subsequently tested phytochemical screening and thin layer chromatography profiles on each extract. Results chromatogram showed the extract containing flavonoids. Testing the activity of the combination of soursop and gotu kola extract performed using seven treatments, combination 1 (SS 1% + GK 7.5% ), a combination of 2 (SS  0.5% + GK 3.75%), the combination of 3 (SS 0, 25% + GK 1.875%), single soursop leaf extract (1%), single gotu kola extract (7.5%), negative control (aquadest) and positive control (Batugin elixir). Kidney stones are then put as much as 100 mg in each treatment and incubated for 6 hours (37 ℃). Results filtrate then didestruksi and dissolved calcium levels measured using Atomic Absorption Spectrophotometer. The results showed the combination soursop leaf ethanol extract and gotu kola leaf extract with a combination of 2 (SS 0.5% + GK 3.75%) is an effective combination of the ability to dissolve calcium kidney stones in vitro.

scholarly journals EKSTRAK AIR TANAMAN SELEDRI (APIUM GRAVEOLENS) TERHADAP PENINGKATAN VOLUME URIN TIKUS PUTIH

2019 ◽  
Vol 10 (3) ◽  
pp. 1-6
Author(s):  
Adita Silvia Fitriana
Keyword(s):  
White Male ◽  
Urine Volume ◽  
Water Extract ◽  
Positive Control ◽  
Negative Control ◽  
Apium Graveolens ◽  
Male Rat ◽  
Preparation Process ◽  
Diuretic Effect ◽  
Made In

ABSTRACT Processing of celery as diuretic is done by society in various ways. So far, the most effective preparation process of celery extract has not been known. This research aims to examine the diuretic effect of various celery extract preparation.  This research was conducted using celery water extract which is made in accordance with the people’s habit, using aquades as a negative control and HCT as a positive control. The effectiveness test of celery water extract as diuretic was done on white male rat by measuring the urine volume every hour for six hours. The results showed that the largest urine volume is obtained from the boiling celery extract.   Keywords: celery, diuretic, rat

Aktivitas Antifungi Air Perasan Bawang Merah (Allium ascalonicum L.) Terhadap Candida albicans Secara In Vitro

2017 ◽  
Vol 9 (2) ◽  
pp. 71
Author(s):  
Nurhasanah Nurhasanah ◽  
Fauzia Andrini ◽  
Yulis Hamidy
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Allium Sativum ◽  
Fungicidal Activity ◽  
Positive Control ◽  
Negative Control ◽  
Randomized Design ◽  
Significant Difference ◽  
Completely Randomized Design

Shallot (Allium ascalonicum L.) has been known as traditional medicine. Shallot which has same genus with garlic(Allium sativum L.) contains allicin that is also found in garlic and has been suspected has fungicidal activity toCandida albicans. It is supported by several researches. Therefore, shallot is suspected has antifungal activity too.The aim of this research was to know antifungal activity of shallot’s water extortion againsts Candida albicans invitro. This was a laboratory experimental research which used completely randomized design, with diffusion method.Shallot’s water extortion was devided into three concentrations, there were 50%, 100% and 200%. Ketoconazole 2%was positive control and aquadest was negative control. The result of this research based on analysis of varians(Anova), there was significant difference between several treatments and was confirmed with Duncan New MultipleRange Test (DNMRT) p<0,05, there was significant difference between 100% shallot’s water extortion with othertreatments, but there was no significant difference between 50% shallot’s water extortion with 200% shallot’s. Theconclusion was shallot’s water extortion had antifungal activity againsts Candida albicans with the best concentration100%, but it was lower than ketoconazole 2%.

scholarly journals Investigation of potential anti-urolithiatic activity from different types of Musa pseudo-stem extracts in inhibition of calcium oxalate crystallization

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Mazni Abu Zarin ◽  
Joo Shun Tan ◽  
Paramasivam Murugan ◽  
Rosma Ahmad
Keyword(s):  
Calcium Oxalate ◽  
Incubation Time ◽  
Positive Control ◽  
Negative Control ◽  
Aggregation Assay ◽  
Banana Tree ◽  
Methanolic Extracts ◽  
Different Types ◽  
Aggregation Activity

Abstract Background The banana or scientifically referred to as Musa sp., is one of the most popular fruits all over the world. Almost all parts of a banana tree, including the fruits, stem juice, and flowers are commonly used as traditional medicine for treating diarrhoea (unripe), menorrhagia, diabetes, dysentery, and antiulcerogenic, hypoglycemic, antilithic, hypolipidemic conditions, plus antioxidant actions, inflammation, pains and even snakebites. The study carried out was to evaluate in vitro anti-urolithiatic activity from different types of Musa pseudo-stems. Methods Observing anti-urolithiathic activity via in vitro nucleation and aggregation assay using a spectrophotometer followed by microscopic observation. A total of 12 methanolic extracts were tested to determine the potential extracts in anti-urolithiasis activities. Cystone was used as a positive control. Results The results manifested an inhibition of nucleation activity (0.11 ± 2.32% to 55.39 ± 1.01%) and an aggregation activity (4.34 ± 0.68% to 58.78 ± 1.81%) at 360 min of incubation time. The highest inhibition percentage in nucleation assay was obtained by the Musa acuminate x balbiciana Colla cv “Awak Legor” methanolic pseudo-stem extract (2D) which was 55.39 ± 1.01%at 60 min of incubation time compared to the cystone at 30.87 ± 0.74%. On the other hand,the Musa acuminate x balbiciana Colla cv “Awak Legor” methanolic bagasse extract (3D) had the highest inhibition percentage in the aggregation assay incubated at 360 min which was obtained at 58.78 ± 1.8%; 5.53% higher than the cystone (53.25%).The microscopic image showed a great reduction in the calcium oxalate (CaOx) crystals formation and the size of crystals in 2D and 3D extracts, respectively, as compared to negative control. Conclusions The results obtained from this study suggest that the extracts are potential sources of alternative medicine for kidney stones disease.

scholarly journals Evaluation of ionic degradation and slot corrosion of metallic brackets by the action of different dentifrices

2013 ◽  
Vol 18 (1) ◽  
pp. 86-93
Author(s):  
Gustavo Antônio Martins Brandão ◽  
Rafael Menezes Simas ◽  
Leandro Moreira de Almeida ◽  
Juliana Melo da Silva ◽  
Marcelo de Castro Meneghim ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Artificial Saliva ◽  
Positive Control ◽  
Negative Control ◽  
Sem Analysis ◽  
Wilcoxon Test ◽  
Surface Polishing ◽  
Aluminium Ion ◽  
Negative Controls

OBJECTIVE: To evaluate the in vitro ionic degradation and slot base corrosion of metallic brackets subjected to brushing with dentifrices, through analysis of chemical composition by Energy Dispersive Spectroscopy (EDS) and qualitative analysis by Scanning Electron Microscopy (SEM). METHODS: Thirty eight brackets were selected and randomly divided into four experimental groups (n = 7). Two groups (n = 5) worked as positive and negative controls. Simulated orthodontic braces were assembled using 0.019 x 0.025-in stainless steel wires and elastomeric rings. The groups were divided according to surface treatment: G1 (Máxima Proteção Anticáries®); G2 (Total 12®); G3 (Sensitive®); G4 (Branqueador®); Positive control (artificial saliva) and Negative control (no treatment). Twenty eight brushing cycles were performed and evaluations were made before (T0) and after (T1) experiment. RESULTS: The Wilcoxon test showed no difference in ionic concentrations of titanium (Ti), chromium (Cr), iron (Fe) and nickel (Ni) between groups. G2 presented significant reduction (p < 0.05) in the concentration of aluminium ion (Al). Groups G3 and G4 presented significant increase (p < 0.05) in the concentration of aluminium ion. The SEM analysis showed increased characteristics indicative of corrosion on groups G2, G3 and G4. CONCLUSION: The EDS analysis revealed that control groups and G1 did not suffer alterations on the chemical composition. G2 presented degradation in the amount of Al ion. G3 and G4 suffered increase in the concentration of Al. The immersion in artificial saliva and the dentifrice Máxima Proteção Anticáries® did not alter the surface polishing. The dentifrices Total 12®, Sensitive® and Branqueador® altered the surface polishing.

In vitro insecticidal effect of commercial fatty acids, ß-sitosterol and rutin against the sugarcane aphid, Melanaphis sacchari Zehntner (Hemiptera: Aphididae)

2022 ◽  
Author(s):  
Liliana Aguilar Marcelino ◽  
Jesús Antonio Pineda Alegría ◽  
David Osvaldo Salinas-Sánchez ◽  
Víctor Manuel Hernández Velázquez ◽  
Gonzalo Iván Silva Aguayo ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Positive Control ◽  
Negative Control ◽  
Tween 20 ◽  
Agricultural Pests ◽  
Melanaphis Sacchari ◽  
Insecticidal Effect ◽  
Sugarcane Aphid ◽  
Alternative Means

The sugarcane aphid, Melanaphis sacchari Zehntner (Hemiptera: Aphididae), is the main pest of sorghum, Sorghum bicolor L. Moench (Poaceae), in Mexico. To control this insect, farmers currently use synthetic chemical insecticides, which are toxic to humans and biodiversity. However, natural products are a promising potential source of safer alternative means to control different agricultural pests. The main objective of this study was to evaluate the insecticidal effect of contact by fumigation of pure molecules of four commercial fatty acids (palmitic, stearic, pentadecanoic and linoleic acids), the phytosterol ß -sitosterol, and the flavonoid rutin. The results showed that fatty acids were the most effective against M. sacchari ; the highest mortality rate (85%) was produced by linoleic acid and the LC 50 was 1,181 ppm, followed by stearic and palmitic acids with mortality percentages of 74 and 63%, respectively, at a concentration of 2,500 ppm at 72 h. The positive control, imidacloprid, had 100% mortality in 24 h and the tween 20 negative control exhibited 4% mortality in 72 h. Our results show that commercial fatty acids are effective against adults of M. sacchari , and can be considered an environmentally friendly alternative to the frequent use of synthetic chemical insecticides.

scholarly journals Evaluation of coconut water neutralized by different agents on the viability of human fibroblasts: an in vitro study

2016 ◽  
Vol 45 (4) ◽  
pp. 234-239 ◽  
Author(s):  
Priscilla Barbosa Ferreira SOARES ◽  
Aletheia Moraes ROCHA ◽  
Manuella Verdinelli de Paula REIS ◽  
Camilla Christian Gomes MOURA ◽  
Carlos José SOARES
Keyword(s):  
Cell Viability ◽  
Tap Water ◽  
Human Fibroblasts ◽  
In Vitro Study ◽  
Positive Control ◽  
Negative Control ◽  
Coconut Water ◽  
Ph Adjustment ◽  
Adjustment Method

Abstract Objective This study evaluated four types of pH adjustment of the coconut water (CW) on viability of human fibroblasts (HFF). Material and method Natural and industrialized CW were adjusted to pH 7.0 using: (1) Sodium Hidroxide (NaOH), (2) Sodium bicarbonate (NaHCO3), (3) Triethanolamine (C6H15NO3), (4) 2-Amino-2-Methil-1-Propanol (C4H11NO). Fibroblasts were plated at 2×104/ well in 96 well plates and maintained in the CW solutions for 2 h and 4 h. Positive control was represented by HFF maintained in DMEM and the negative control by tap water. Cell viability was analyzed by MTT formazan method. Data were analyzed by 3-way ANOVA followed by Tukey’s and Dunnet’s test. Result There are no significant effect on the cell viability regarding type of CW, period of evaluation, and the interactions between CW and period of evaluation, CW and pH adjustment method, pH adjustment method and period of evaluation (p>0.05). Conclusion The product used for CW pH adjustment did not influenced HFF viability, thought there are a tendency of better performance in natural CW.

scholarly journals Pengaruh Pemberian Ekstrak Teripang Pasir terhadap Hambatan Pertumbuhan Shigella Dysentriae Secara In Vitro

2020 ◽  
Vol 18 (1) ◽  
pp. 47
Author(s):  
FERIZAL NEGERI SAMUDRA ◽  
RETNO BUDIARTI ◽  
IRMAWATI IRMAWATI
Keyword(s):  
Sea Cucumber ◽  
Antibacterial Effect ◽  
Positive Control ◽  
Negative Control ◽  
Shigella Dysenteriae ◽  
Control Group ◽  
Control Groups ◽  
Shigella Spp ◽  
Diarrhea Disease

<p><strong>ABSTRACT</strong></p><p><strong>Background</strong>; In Indonesia, most diarrhea disease in 1995 to 2001 are caused by Shigella spp. Shigella spp infection can cause various symptom dan complication. Generally, the treatment by using antibiotic can cause antibiotic resistance. Sea cucumber (Holoturia scabra) is an herb that known, available, and easy to consume by society and has an antibacterial effect. Therefore, further research to study the effect of Holoturia Scabra on <em>Shigella Dysentriae</em> growth in vitro is needed.</p><p><strong>Objectives</strong>: The goal of this research is demonstrate the effect of sea cucumber (Holoturia scabra) to the growth of the <em>Shigella dysentriae</em> bacteria in vitro.</p><p><strong>Method</strong>: The method in this research is Posttest Only Control Group. There are 6 groups, 4 types of and 2 control groups. The concentration of the treatment group is 100%,50%, 25%, and, 12.5% while for positive control tests using chloramphenicol and aquadest as a negative control.</p><p><strong>Result</strong>: The result showed there is an influence on the intake of sand cucumber to the growth of the Shigella dysenteriae.</p><p><strong>Conclusion</strong>: Sea cucumber (<em>Holoturia scabra</em>) inhibit the growth of <em>Shigella dysenteriae</em>.</p><p><strong>Key words</strong>: <em>Shigella dysenteriae</em>, sea cucumber (<em>Holoturia scabra</em>), antibacterial</p>

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