scholarly journals Hematological and Biochemical parameters study of female albino rats treated with lamotrigine drug

2019 ◽  
Vol 24 (3) ◽  
pp. 23
Author(s):  
Gawhar Ahmed Shekha ◽  
Kalthum Asaaf Maulood
Keyword(s):  
Body Weight ◽  
Biochemical Parameters ◽  
Haemoglobin Concentration ◽  
Chloride Ion ◽  
Treated Group ◽  
Female Rats ◽  
Control Group ◽  
Albino Rats ◽  
Anti Epileptic Drug ◽  
Hematological And Biochemical Parameters

The present study was aimed to investigate the possible effects of the anti-epileptic drug lamotrigine (LTG) on some haematological and biochemical parameters in adult female rats. Forty-eight female rats were divided into three groups (each group=16). Group one can be considered as a control group, group two and three administrated lamotrigine drugs orally at a dose of 3.57mg/kg body weight and 7.14mg/kg body weight for 7,14,21,28 day and all groups fed with standard rat feed. The results showed that there were significant (P≤0.05) changes in haematological parameters in group two and three when compared with the control group during all period except the mean level of corpuscular haemoglobin concentration (MCHC). The liver enzyme aspartate transaminase (AST) and alanine transaminase (ALT) and serum urea, creatinine with calcium, potassium, sodium and chloride ion showed significant alteration in the treated group, the relative organ weight showed significant changes in group two and three in comparison with control group during 7,14,21 and 28 days. Estradiol level in group three increased at 7, 14 and 21 day and decreased at 28 days of treatment when compared with group two and the control group. This study suggested that treatment of healthy female albino rats with therapeutic doses of lamotrigine drug for 28 days generally affect on included parameters in this study.   http://dx.doi.org/10.25130/tjps.24.2019.044

Effect of Apricot kernel on Some Hematological,Histological and Biochemical Parameters in CCl4-induced Liver Injury in rats

2018 ◽  
Vol 2 (1) ◽  
Author(s):  
Firdaws AL-Mashhadani ◽  
Abbas QadirSalihi ◽  
Nazdar Wasman
Keyword(s):  
Liver Injury ◽  
Biochemical Parameters ◽  
Paraffin Section ◽  
Histological Section ◽  
Treated Group ◽  
Normal Diet ◽  
Control Group ◽  
Albino Rats ◽  
Apricot Kernel ◽  
Hematological And Biochemical Parameters

The purpose of the present study was to find out the effect of apricot kernel on carbon tetrachloride (CCl4) induced liver injury in male albino rats. To achieve this purpose and to explain, the histological, hematological and biochemical parameters were included. Thirty male albino rats were used and randomly divided to in to three groups. The first group represented control group which received normal diet and intraperitoneal injection with oil (0.5 ml/kg). The second  group injected with CCl4(1 ml/kg), represented the CCl4(1 ml/kg)model,the third groupwere treated like the second group with 15%/ day apricot kernelfor a month . The  apricot kernel extracts treated group showed significant differences in AST, ALT, ALP, direct bilirubin, , GSH, liver SOD, WBC, LYM and PLT when compared to CCl4 treated rats. In the current study histological section through the liver of control rats showed normal architecture of hepatocytes.Although Paraffin section of the rat liver treated with CCl4 most of the hepatocytes were degenerated, congestion of blood and inflammatory leukocyte infiltration were observed,the results showed that CCl4 injection caused significant alterations in histological and biochemical parameters but our study showed that the apricot kernel administration showed improvement of histological analysis for four weeks (March to April 2015).

scholarly journals Diuretic activity of the bark of Eysenhardtia polystachya

2016 ◽  
Vol 11 (1) ◽  
pp. 212 ◽  
Author(s):  
Saudy Saret Pablo-Pérez ◽  
María Mirian Estévez-Carmona ◽  
María Estela Meléndez-Camargo
Keyword(s):  
Body Weight ◽  
Filtration Rate ◽  
Treated Group ◽  
Female Rats ◽  
Control Group ◽  
Electrolyte Balance ◽  
Urinary Flow ◽  
Diuretic Activity ◽  
Sodium And Potassium ◽  
Different Doses

<p class="Abstract">The aim of this study was to evaluate the diuretic activity of <em>Eysenhardtia polystachya</em> bark aqueous extract at different doses in a rat model. Different doses of <em>E. polystachya</em> (125, 250, 500 and 750 mg/kg body weight), furosemide (4 mg/kg) and vehicle were administered per os to female rats (n=6 animals per group). After 6 hours in metabolic cages, the effect on urinary flow, glomerular filtration rate and electrolyte balance of sodium and potassium were assessed in all animals. <em>E. polystachya</em> at the doses of 500 and 750 mg/kg induced diuretic activity, since markedly increased (p&lt;0.05) the urinary flow rate, similar to that of furosemide treated group. Only the dose of 750 mg/kg produced an increment in urinary excretion of sodium but not of potassium compared with control group. These findings indicate that<em> E. polystachya</em> bark-induced diuretic activity, providing evidence for its folkloric use.</p><p> </p>

scholarly journals Anti-hyperglycemic Effects of Diacerein in Alloxan-Induced Diabetes Mellitus in Wistar Albino Rats

2020 ◽  
pp. 107-113
Author(s):  
Arsalan Uqaili ◽  
Samia Siddiqui ◽  
Roomi Aijaz ◽  
Yar Muhammad Nizammani ◽  
Navaid Kazi ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Body Weight ◽  
Blood Glucose ◽  
Treated Group ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Albino Rats ◽  
Group B ◽  
Group D

Objective: To determine the anti-hyperglycemic effects of interleukin-1 inhibitor (diacerein) in alloxan induced diabetic albino wistar rats. This experimental study was performed at the Department of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University, Tando Jam within 6 months from April 2016 to September 2016. Total of 160 adult Albino Wistar Rats having an average of 200 to 300 grams body weights were selected. Animals were categorized into 4 groups as; Group A (n=15): Control rats – receive 0.9% normal saline as placebo Experimental Groups Group B (n=15): Experimental Control (Diabetic rats) - Alloxan50 mg/kg body weight intraperitoneal. Group C (n=15): Diabetic rats + Diacerein (30 mg/kg/day) orally daily. Group D (n=15): Diabetic rats + Diacerein (50 mg/kg/day) orally daily. Animals were kept and treated as per the NIH Guideline for Use and Care of Laboratory Animals. Diabetes mellitus was induced via a single intraperitoneal injection of 50 milligram/kg alloxan monohydrated dissolved in aseptic 0.9% saline. After 72 hours, blood specimens were taken from the caudal vein of the rats and glucose level>200 mg/dL was taken as diabetes. Experimental rats were given diacerein approximately 30 and 50 mg orally for 6 weeks. At the completion of experiment the body weight was measured of each animal by electronic measuring balance and blood sample was taken from each animal of all groups to assess the blood glucose level and HbA1c level. Data were recorded via self-made proforma and analysis was done by using SPSS version 20. Results: Average body weight of Diabetic control (Group B) was 193.33±22.50 grams, which was lower in contrast to Diacerein treated group C 202.47±25.70 grams and significantly lower as compared to Diacerein treated group D as  212.6±23.43 grams. A significant increase in blood glucose levels 182.07±10.63 mg/dl was noted in the Diabetic control (Group B) compared to Diacerein treated group C (110.13± 8.54 mg/dl) and group D (85.87±8.41 mg/dl) (P=0.001). HbA1c was markedly raised in the Group B- diabetic controls, while diacerein treated diabetic rats (groups C and D) showed a significant decrease in HbA1c (P=0.001). Conclusion: It was concluded that Diacerein achieves the Euglycemic state by reducing the levels of blood glucose and glycated hemoglobin (HbA1c) in Alloxan-Induced diabetes mellitus in Wistar Albino Rats.

scholarly journals Effects of Sweet Potato Leaves, Roselle Calyces and Beetroot on Body Weight, Selected Hematological and Biochemical Parameters in Broiler Chicken

2019 ◽  
Vol 10 (2) ◽  
pp. 133
Author(s):  
Gaymary George Bakari ◽  
Robert Arsen Max ◽  
Shedrack Reuben Kitimu ◽  
Shaabani Mshamu ◽  
Benigni Alfred Temba ◽  
...  
Keyword(s):  
Body Weight ◽  
Sweet Potato ◽  
Biochemical Parameters ◽  
Broiler Chickens ◽  
Hemoglobin Concentration ◽  
The Other ◽  
Control Group ◽  
Positive Effects ◽  
Hematological And Biochemical Parameters ◽  
Sweet Potato Leaves

The study was conducted to assess the effects of sweet potato leaves, roselle calyces and beetroot tubers on body weight, selected hematological and biochemical parameters in broiler chickens. Eighty four (84) broiler chickens aged four weeks were randomly assigned into six groups of 14 chickens each. The first group (G0) remained as untreated control while the other groups were the treated groups which received 25% of ground sweet potato leaves (G1), rosella calyces (G2), beetroot tubers (G3) and their mixtures (G4 and G5) for 28 days. Following inclusion of these different vegetables, blood samples were collected and analyzed for selected hematological and biochemical parameters on day 0, 7, 14, 21 and 28. Results showed that consumption of the three vegetables caused significant decreases (p < 0.05) in body weight, serum glucose and cholesterol. On the other hand, hemoglobin concentration, packed cell volume (PCV) and total white blood cell (WBC) counts were shown to increase significantly (p < 0.05) compared to the control group. It is concluded that the hypoglycemic and hypocholesteremic effects in chickens following consumption of the three vegetables together with their positive effects on PCV and WBC counts are important qualities which can be utilized in the management of conditions such as obesity, diabetes mellitus, hyperlipidemia and cardiovascular diseases (atherosclerosis and coronary disease) in humans. Further studies in other animals are recommended.

Effects of Curcuma Zedoaria (Shoti) on Growth Performance and Hemato-Biochemical Parameters in Broiler

2020 ◽  
Vol 7 (2) ◽  
pp. 293-301
Author(s):  
TM Rahsin Kabir ◽  
Nahid Nawrin Sultana ◽  
Tangila Ferdausi ◽  
Muhammad Rakibul Hasan ◽  
Nayamul Bashar ◽  
...  
Keyword(s):  
Body Weight ◽  
Biochemical Parameters ◽  
Biochemical Analysis ◽  
Treated Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Broiler Chicks ◽  
Collect Blood Sample ◽  
Growth Performances

The experiment was conducted on “Cobb-500” broiler chicks to study the effects of Curcumazedoaria (shoti) on growth performances and haemato-biochemical parameters. A total of twentybroilers chicks (16 days old) were randomly divided into five equal groups (n=4). Three groups ofbroilers fed shoti powder at the rate of 10%; 20%; 30% (w/w) with other ingredients for 22 days.Broilers chicks fed on maize-soybean were considered as positive control while non-protein controlwas regarded as negative control. The results showed that body weight of broilers fed 10% and 20%shoti meal grew very close to those of control (maize-soybean) group. After having completed thefeeding trial, the birds were sacrificed to collect blood sample for and biochemical analysis .Weightsof different organs and length of intestine were also measured. Results showed that decreaseddressed weight, weight of skin and liver were found in 30% shoti treated group compared to proteincontrol group. Intestinal weight was highest in 30% shoti treated group but length was similar to theprotein control group. Total erythrocytes count, hemoglobin content and PCV value were founddecreased in shoti treated group compared to protein control group but ESR value was highest in10% shoti treated group. Total cholesterol was found to be decreased in the birds fed on shoticompared to protein control and significantly higher than non-protein control group. Triglyceride valuedecreased in 10% shoti treated group compared to protein and non-protein control group. IncreasedHDL value was found in 10% shoti treated group compared to control groups and 20 % and 30 %shoti treated groups. Result of this experiment showed that body weight of birds significantly (p<0.05)increased in all shoti treated groups. Further investigations are necessary by using differentpercentages of shoti and other shoti type feed to determine the effects of this feed supplement onanimal’s physiology. Res. Agric., Livest. Fish.7(2): 293-301,  August 2020

scholarly journals TOCOSH FLOUR (Solanum tuberosum L.): A Toxicological Assessment of Traditional Peruvian Fermented Potatoes

Foods ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 719
Author(s):  
Jonas Roberto Velasco-Chong ◽  
Oscar Herrera-Calderón ◽  
Juan Pedro Rojas-Armas ◽  
Renán Dilton Hañari-Quispe ◽  
Linder Figueroa-Salvador ◽  
...  
Keyword(s):  
Body Weight ◽  
Acute Toxicity ◽  
Biochemical Parameters ◽  
Solanum Tuberosum L ◽  
Lethal Dose ◽  
Clinical Signs ◽  
Control Group ◽  
Toxicological Assessment ◽  
Hematological And Biochemical Parameters ◽  
Histopathological Studies

Potato tocosh is a naturally processed potato for nutritional and curative purposes from traditional Peruvian medicine. The aim of this study was to investigate the acute and sub-acute toxicity of tocosh flour (TF). For sub-acute toxicity, TF was administered orally to rats daily once a day for 28 days at doses of 1000 mg/kg body weight (BW). Animals were observed for general behaviors, mortality, body weight variations, and histological analysis. At the end of treatment, relative organ weights, histopathology, hematological and biochemical parameters were analyzed. For acute toxicity, TF was administered orally to mice at doses of 2000 and 5000 mg/kg BW at a single dose in both sexes. Body weight, mortality, and clinical signs were observed for 14 days after treatment. The results of acute toxicity showed that the median lethal dose (LD50) value of TF is higher than 2000 g/kg BW but less than 5000 mg/Kg BW in mice. Death and toxicological symptoms were not found during the treatment. For sub-acute toxicity, we found that no-observed-adverse-effect levels (NOAEL) of TF in rats up to 1000 g/kg BW. There were statistically significant differences in body weight, and relative organ weight in the stomach and brain. No differences in hematological and biochemical parameters were observed when compared with the control group. For sub-acute toxicity, histopathological studies revealed minor abnormalities in liver and kidney tissues at doses of 5000 mg/Kg. Based on these results, TF is a traditional Peruvian medicine with high safety at up to 1000 mg/kg BW for 28 days in rats.

scholarly journals ACUTE DERMAL TOXICITY STUDY OF ARECA CATECHU LINN. EXTRACT IN SPRAGUE-DAWLEY RATS

2016 ◽  
Vol 9 (9) ◽  
pp. 209
Author(s):  
Liza Meutia Sari ◽  
Frans D Suyatna ◽  
Gus Permana Subita ◽  
Elza Ibrahim Auerkar
Keyword(s):  
Body Weight ◽  
Aqueous Extract ◽  
Lethal Dose ◽  
Clinical Signs ◽  
Treated Group ◽  
Female Rats ◽  
Control Group ◽  
Sprague Dawley ◽  
Dermal Toxicity ◽  
Areca Catechu

ABSTRACTObjective: Areca catechu Linn. or biji pinang is one of the most widely used psychoactive substance with several hundred million users worldwide,predominantly in Southern Asia. However, details of the dermal toxicity of A. catechu L. are still undiscovered. The objective of this study is toinvestigate the in vivo acute dermal toxicity of aqueous extract of A. catechu L. at dose 15,000 mg/kg body weight in Sprague-Dawley rats.Methods: The acute dermal toxicity of A. catechu L. nut extract was investigated in rats, as per OECD Guidelines 402 for acute toxicity protocols. Thebody weight, possibility of death, general signs, and behavior activity parameters were measured for 14 days to ascertain the median lethal dose(LD50) of the extract. At the end of the study, all the animals in all the treated group were sacrificed.Results: The LD50 was found to be >15,000 mg/kg body weight. There was significant weight increase (p<0.05) in treated group when comparedto control group. No mortality was observed during whole 14 days study period. A single dose of 15,000 mg/kg of body weight did not producetreatment-related signs of toxicity in any of animal tested.Conclusion: A single dermal dose to A. catechu L. aqueous extract had no toxic effects on mortality, clinical signs, body weight changes, and grossfindings in female rats at a dose of 15,000 mg/kg of body weight. Subsequently, the concentrate can be employed for pharmaceuticals nutrient plants.Keywords: A. catechu L., Acute dermal toxicity, LD50.

MELATONIN ON ENDOCRINE DNA CHANGES IN FEMALE RATS AT INCREASING AGES

1971 ◽  
Vol 68 (3) ◽  
pp. 597-604 ◽  
Author(s):  
D. V. Singh ◽  
C. W. Turner
Keyword(s):  
Body Weight ◽  
Treated Group ◽  
The Other ◽  
Female Rats ◽  
Unit Weight ◽  
Control Group ◽  
Uterine Weight ◽  
Endocrine Glands ◽  
Sprague Dawley ◽  
Total Dna

ABSTRACT Sixty female Sprague-Dawley-Rolfsmeyer rats were divided equally into three groups, twenty in each group at 25 days of age. One half of each group serving as controls received saline, the other half received 100 ftg melatonin/100 g body weight at 25, 35. and 45 days of age, for 10 days. They were killed 24 hours after the last injection. Pituitaries. ovaries, uteri, adrenals and thyroids were collected and weighed. DNA determination by the method described by Webb Se Levy (1955) was used for ovaries, uteri and adrenals only. Pituitary weight was not affected much by the treatment of melatonin. Thyroid weight increased: adrenals, ovaries and uterine weight decreased gradually up to 55 days in melatonin treated group as compared to the control. Ovarian total DNA increased significantly at 35 days of age, but significantly decreased at 45 and 55 days of age in melatonin treated group as compared to the corresponding controls. No difference in total DNA in adrenals was found in melatonin treated animals as compared to control group at any age. The total DNA of the uteri increased 29 % at 35 days of age but decreased at 55 days of age by 14%. These data also show that the DNA/mg of endocrine glands dry fat free tissue decreased with increasing age suggesting a reduction in functional cells per unit weight in older animals.

scholarly journals Effect of Resveratrol on Hematological and Biochemical Alterations in Rats Exposed to Fluoride

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Nurgül Atmaca ◽  
Ebru Yıldırım ◽  
Bayram Güner ◽  
Ruhi Kabakçı ◽  
Fatih Sultan Bilmen
Keyword(s):  
Enzyme Activity ◽  
Biochemical Parameters ◽  
Hematological Parameters ◽  
Treated Group ◽  
Inorganic Phosphorus ◽  
Control Group ◽  
Protective Effects ◽  
Biochemical Alterations ◽  
Fluoride Treatment ◽  
Hematological And Biochemical Parameters

We investigated the protective effects of resveratrol on hematological and biochemical changes induced by fluoride in rats. A total of 28 rats were divided into 4 groups: control, resveratrol, fluoride, and fluoride/resveratrol(n=7each), for a total of 21 days of treatment. Blood samples were taken and hematological and biochemical parameters were measured. Compared to the control group, the fluoride-treated group showed significant differences in several hematological parameters, including decreases in WBC, RBC, and PLT counts and neutrophil ratio. The group that received resveratrol alone showed a decrease in WBC count compared to the control group. Furthermore, in comparison to the control group, the fluoride group showed significantly increased ALT enzyme activity and decreased inorganic phosphorus level. The hematological and biochemical parameters in the fluoride + resveratrol treated group were similar to control group. In the fluoride + resveratrol group, resveratrol restored the changes observed following fluoride treatment, including decreased counts of WBC, RBC, and PLT, decreased neutrophil ratio and inorganic phosphorus levels, and elevated ALT enzyme activity. The present study showed that fluoride caused adverse effects in rats and that resveratrol reduced hematological and biochemical alterations produced by fluoride exposure.

scholarly journals Effects ofSalvadora persicaExtract on the Hematological and Biochemical Alterations against Immobilization-Induced Rats

Scientifica ◽  
2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Kholoud S. Ramadan ◽  
Salha A. Alshamrani
Keyword(s):  
Biochemical Parameters ◽  
Hematological Parameters ◽  
Hdl Cholesterol ◽  
Treated Group ◽  
Control Group ◽  
Serum Samples ◽  
Salvadora Persica ◽  
Biochemical Alterations ◽  
Hematological And Biochemical Parameters

A total of 24 rats were divided into 4 groups: control, stress, extract alone, and stress + extract (n=6each), for total 21 days of treatment. The immobilization stress was induced in rats by putting them in 20 cm × 7 cm plastic tubes for 2 h/day for 21 days. Rats were postorally treated withSalvadora persicaat a dose of 900 mg/kg body weight via intragastric intubations. At the end of the test period, hematological and biochemical parameters were determined in blood and serum samples with determination of vital organs weights. The vital organ weights were not significantly affected in stressed rats as compared to control rats. Compared to the control group, the stress treated group showed significances in several hematological parameters, including decreases in WBC, RBC, and PLT counts. Furthermore, in comparison to the control group, the stress group showed significantly increased blood glucose, serum total cholesterol, LDL-cholesterol, and triacylglycerols levels and decreased HDL-cholesterol level. The hematological and biochemical parameters in the stress + extract treated group were approximately similar to control group. The SP extract restored the changes observed following stress treatment.

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