scholarly journals UJI EFEKTIFITAS DAUN PANDAN WANGI (PANDANUS AMARYLLIFOIUM ROXB) SEBAGAI ANTIJAMUR TERHADAP PITYSPORUM OVALE

2021 ◽  
Vol 3 (2) ◽  
pp. 81-85
Author(s):  
Saadah Siregar ◽  
Riana Topia
Keyword(s):  
Fungal Growth ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Oil Glands ◽  
Pityrosporum Ovale ◽  
Agar Media ◽  
Metabolite Content ◽  
Pandanus Amaryllifolius

The Pandan Wangi leaf plant is a plant that belongs to the Pandanaceae family. The metabolite content of fragrant pandan leaves (Pandanus amaryllifolius  Roxb.) Alkaloids, flavonoids, saponins, tannins are known to have an antifungal effect. Pityrosporum ovale is a yeast or single-celled fungus that belongs to the genus Malassezia sp, and belongs to the Cryptococcaceae family. Pityrosporum ovale causes superficial dermatomycosis which affects the stratum corneum in the epidermis layer. This fungus is a normal flora on the scalp, but in conditions of hair with excess oil glands, this fungus can thrive. The purpose of this study was to determine the inhibition zone of fragrant pandanus leaves (Pandanus amaryllifolius Roxb.) Concentrations of 10%, 20%, 30%, and 40% of Pytirosporum ovale on Potato Dextrose Agar media by diffusion method using disc paper. The diffusion method was used to measure the diameter of the zone of inhibition against fungal growth. The positive control used was ketoconazole 2% and negative control DMSO 1%. The results of the study using the diameter of the inhibition zone at a concentration of 10% were 7.86 mm, the inhibition zone at a concentration of 20% was 8.53 mm, the inhibition zone at a concentration of 30% was 8.76 mm, and the inhibition zone at a concentration of 40% was 9, 43 mm.

scholarly journals AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL BIJI KAPULAGA JAWA (Amomum compactum Soland. ex Maton) TERHADAP Streptococcus pyogenes

EKOLOGIA ◽  
2020 ◽  
Vol 20 (1) ◽  
pp. 31-39
Author(s):  
Oom Komala ◽  
. Ismanto ◽  
Muhammad Alan Maulana
Keyword(s):  
Antibacterial Activity ◽  
Streptococcus Pyogenes ◽  
Pathogenic Bacteria ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Dilution Method ◽  
Natural Treatment ◽  
Amomum Compactum

Streptococcus pyogenes is one of the pathogenic bacteria that causes pharyngitis. Natural treatment to overcome these problems is to use cardamom seeds. The purpose of this study was to test the antibacterial activity, determine the concentration of inhibitory zone and phytochemical compounds from  ethanol 96% extract of Java cardamom seeds (Amomum compactum Soland. Ex Maton) against Streptococcus pyogenes. The method is used   solid dilution and paper disc diffusion method. The solid dilution method is used for the Minimum Inhibitory Concentration (MIC) test with a concentration of 1.25%, 2.5%, 5% and 7.5% while the paper diffusion method is used for the Inhibition zone Diameter (IZD)  using five treatments namely three concentrations of ethanol 96% extract of Java cardamom seeds (7.5%, 10% and 12%), one positive control of amoxicillin 0.01 mg/mL and one negative control of sterile distilled water. IZD data were  analyzed using ANOVA with a confidence level of 95% and α = 0.05 and Duncan's further tests to determine differences between treatments. The results showed that the MIC  was at a concentration of 7.5% while for the IZD test which had the highest activity there was a concentration of 12% with an average inhibition diameter of 12.03 ± 0.14 mm. In addition, ethanol 96% extract of Java cardamom seeds contain alkaloids, flavonoids, terpenoids and tannins which function in antibacterial activity.

scholarly journals The Potential of Mango (Mangifera infica L.) Peel of Apple Varieties By Infusion And Maceration In Inhibiting Pseudomonas aeruginosa And Propionibacterium acnes

2021 ◽  
Vol 4 (1) ◽  
pp. 1-6
Author(s):  
Vifin Putri Rahmawati ◽  
Chylen Setiyo Rini
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Mangifera Indica ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Chemical Components ◽  
Extract Concentration ◽  
Potential Test ◽  
Antibacterial Potential

Plants have many chemical components. The use of natural ingredients as an alternative treatment in dealing with diseases, especially acne. One of them is mango (Mangifera indica L.) varieties of apples obtained at the Larangan Main Market in Sidoarjo. This study aims to determine the potential of infusion and maceration of mango skin varieties in inhibiting Pseudomonas aeruginosa and Propionibactrium acne at various concentrations. This antibacterial potential test was carried out using the diffusion method of the wells. The antibacterial potential is characterized by the formation of a clear zone around the well called the inhibition zone. This study uses 10 concentrations namely 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% and 100% and Clindamycin as positive control and aquades as negative control. Based on the results of the Two Way ANOVA test data obtained were not normally distributed, therefore a comparison test was performed using the Kruskal-Wallis test with a sign value (α <0.05). This showed that there were significant differences in the use of various concentrations. The maceration extract concentration of 100% is the best concentration to form a zone of inhibition against P. aeruginosa  of 17.9 mm and P. acne bacteria of 13.2 mm. The results of the infusion extract concentration did not form inhibitory zones in both of P. aeruginosa and P. acnes.

scholarly journals EFFECTIVENESS OF ANTIBACTERIAL EXTRACT OF KENOP (GOMPHRENA GLOBOSA) FLOWER EXTRACT AGAINST GROWTH OF PROPIONIBACTERIUM ACNES BACTERIA

2020 ◽  
Vol 4 (2) ◽  
pp. 115
Author(s):  
Elvina Veronica ◽  
Sang Ayu Arta Suyantari ◽  
Widia Danis Swari ◽  
Ni Made Ari Purwaningrum ◽  
Agung bagus sista Satyarsa ◽  
...  
Keyword(s):  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Treatment Groups ◽  
Gomphrena Globosa ◽  
Mueller Hinton ◽  
Mueller Hinton Agar ◽  
Herbal Plants ◽  
Agar Media ◽  
Inhibitory Power

Acne is a chronic inflammation of the pilosebaceous tissue caused by the bacterium Propionibacterium acne (P.acnes) which often experiences antibiotic resistance. Research on the benefits of herbal plants in dealing with acne is still needed, one of which is the use of Knobs flowers (Gomphrena globosa) which is easily found and has potential as an antioxidant and antibacterial. This study aims to determine the effectiveness of the extract of the flower knob in inhibiting the growth of P.acnes bacteria. The design of this study was purely experimental with the Kirby-Bauer method using MHA (Mueller Hinton Agar) media. A positive control using clindamycin antibiotics and negative control using aquadest. The treatment group consisted of 25%, 50%, 75%, and 100% knob methanol extract with 3 replications. Data analysis using One Way ANOVA to find out the average difference between treatment groups. Based on the results of the study, it was found that the higher the concentration of the extract, the greater the inhibitory power as long as the extract had not reached saturated concentration (p <0.05). There is a decrease in the inhibition zone diameter at 100% concentration because it is influenced by the diffusion rate of a compound and other factors.

scholarly journals 25. The effect of Fingerroot Rhizome (Boesenbergia pandurata) Extract on the Growth of Staphylococcus aureus in Vitro

2019 ◽  
Vol 13 (2) ◽  
Author(s):  
Silvia P.N Keliat ◽  
Darniati Darniati ◽  
Abdul Harris ◽  
Erina Erina ◽  
Rinidar Rinidar ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Average Diameter ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Final Conclusion ◽  
Distilled Water

This study aimed to determine the inhibitory effect and the concentration of fingerroot extract to inhibit Staphylococcus aureus growth. The data was analyzed descriptively. Ampicillin was used as positive control, distilled water was used as negative control, and the treatments were given fingerroot extract with a concentration of 5%, 15%, 25%, 35% and 45%. This study was conducted with three replications. The parameter measured was the diameter of inhibition zone formed by diffusion method. The average diameter of inhibition zone of the fingerroot extract were 15% : 10.3 mm; 25% : 13,6mm; 35% : 18,7mm; 45% ± 21,1mm, and at a concentration of 5% the inhibition zone is not formed. The final conclusion is that the fingerroot extract can inhibit the growth of Staphylococcus aureus. The higher concentration of fingerroot extract, the more extensive inhibition zone formed.

IDENTIFIKASI SENYAWA METABOLIT SEKUNDER DAN UJI AKTIVITAS EKSTRAK ETANOL HERBA MENIRAN (Phyllanthus niruri L.) TERHADAP CANDIDA ALBICANS MENGGUNAKAN METODE DIFUSI CAKRAM

2017 ◽  
Vol 3 (1) ◽  
pp. 1
Author(s):  
Anita Apriliana ◽  
Eko Kusumawati ◽  
Selvitawati Selvitawati
Keyword(s):  
Candida Albicans ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
P Value ◽  
Phyllanthus Niruri ◽  
Significant Difference ◽  
Agar Media ◽  
One Way Anova

The aim of this research is to know the ability of meniran ethanol extract (Phyllanthus niruri L.) in inhibiting the growth of Candida albicans. The research design is an experimental study. The extract used was herbal meniran extract made by maceration using 70% ethanol solvent, the content of ethanol extract of meniran tested against Candida albicans using disc diffusion method were 5%, 10%, 20%, 30% and 40%. Activity test conducted on Sabaraound Dextrose agar media that has been smeared with Candida albicans culture which has been standardized with Mc Farland standard. The pre-tested disc paper is first dipped into the extract with various concentrations. Incubated at 30 ° C for 24 hours and the inhibit zone formed was measured. The result showed that herbal ethanol extract of meniran resulted in drag zone diameter at Candida albicans ie 8,5 mm; 10.3 mm; 12.6 mm; 14.1 mm and 14.3 mm, for negative control 0 mm and positive control 20.1 mm. Data obtained from the results of the study were analyzed using one way anova method showed p - value <0,05, it was concluded that there was significant difference between negative control, 5%, 10%, 20%, 30%, 40% and positive control.

scholarly journals Lime Peel Liquid (Citrus aurantifolia, Swingle) Inhibit Escherichia Coli In Vitro

2021 ◽  
Vol 4 (1) ◽  
pp. 63
Author(s):  
Devi Indah Sari ◽  
Retno Sri Wahjuni ◽  
Ratih Novita Praja ◽  
Budi Utomo ◽  
Faisal Fikri ◽  
...  
Keyword(s):  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Citrus Aurantifolia ◽  
E Coli ◽  
Randomized Design ◽  
Mueller Hinton ◽  
Lime Peel

The purpose of this study was to determine in vitro inhibition test of lime peel liquid (Citrus aurantifolia, Swingle) against E. coli. The E. coli isolate was cultured on Muller Hinton Agar (MHA) media. Antibacterial test in this study using diffusion method. Negative control (K-) using CMC-Na 0.1%. Positive control (K+) using oxytetracycline antibiotics. The treatments were given lime peel liquid (Citrus aurantifolia, Swingle) with concentrations of 100%, 75%, 50% and 25%, respectively. The study design using Completely Randomized Design (CRD) method, with six treatments and four repetitions. Data were analyzed by Kruskal-Wallis test, Moreover, the Post Hoc Mann-Whitney method. The inhibition zone analysis results were showed that K + had significant differences compared to all treatments K-, 100%, 75%, 50%, and 25%. K- was found to be significantly different from treatments 100%, 75%, 50% and 25%. The results showed that the lime peel liquid has the potential as an antibacterial activity against the growth of E. coli in vitro. Concentration of 25% has shown the presence of inhibitory zones on Mueller Hinton media that have been inoculated with lime peel liquid.

scholarly journals UJI EFEKTIVITAS ANTIBAKTERI EKSTRAK DAUN SIRSAK (ANNONA MURICATA LINN.) TERHADAP BAKTERI PROPIONIBACTERIUM ACNES

2019 ◽  
Vol 6 (1) ◽  
pp. 65
Author(s):  
Yurlina Zai ◽  
Agnes Yohana Kristino ◽  
Sri Lestari Ramadhani Nasution ◽  
Oliviti Natali
Keyword(s):  
Leaf Extract ◽  
Propionibacterium Acnes ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Annona Muricata ◽  
Research Model ◽  
Negative Controls

This research aims to determine the effectiveness of the antibacterial soursop leaf extract (Annona muricata L.). The method used is the agar diffusion method. The leaves were extracted by maceration method, using ethanol as a solvent. The soursop leaf extract obtained was then diluted using ethanol 96% at a concentration of 20%, 40%,60%, 80%. Positive control using clindamycin and negative controls using sterile aquadest. The research model used in this study is an experimental research model In Vitro of type. The results showed that soursop leaf extract had antibacterial effectiveness against Propionibacterium acnes with an average inhibition zone diameter of 9,7 mm; 13,7 mm; 15,7 mm; 16,3 mm; positive control (clindamysin) 21,0 mm; and negative control (aquadest) have no value tobe able to inhibit bacterial growth.

scholarly journals Single Clove Garlic (Allium sativum) Essential Oil as an Inhibitor of Staphylococcus aureus Bacteria

2019 ◽  
Vol 11 (1) ◽  
pp. 77-83
Author(s):  
Abdul Gofur ◽  
Ida Wulandari ◽  
M. Fitri Athoillah ◽  
Agung Witjoro ◽  
Sri Rahayu Lestari
Keyword(s):  
Staphylococcus Aureus ◽  
Essential Oil ◽  
Morphological Structure ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Bacterial Cells ◽  
Test Analysis ◽  
Garlic Essential Oil

Staphylococcus aureus is gram-positive bacteria that often infect the skin. S. aureus has been experiencing resistance to several antibiotics. One of the solutions to overcome the resistance is by using garlic that is believed by the society can overcome bacterial infection. The study aimed to find out the influence of the variation in concentration of local single clove garlic essential oil used on the inhibition and damage of morphological structure of S. aureus bacteria. The inhibition test was conducted using disc diffusion method. The experiment groups consisted of  1% DMSO as negative control, vancomycin 30µg/ml as positive control and single clove garlic essential oil (25 mg/ml, 50 mg/ml, 75 mg/ml and100 mg/ml) as treatment groups. The diameter of inhibition zone was measured using calipers. The morphological damage of the bacterial cells can be seen using Scanning Electron Microscopy (SEM) with magnification of 25000x. The result of Kruskal-Wallis test analysis indicated that the extract of local garlic essential oil has inhibitory activities against S. aureus bacteria (P<0.05). The damage to the morphological structure of bacterial cells with the administration of 30 µg/ml vancomycin was equal to 100 mg/ml single clove garlic essential oil extract. Single clove garlic essential oil can be used as an alternative treatment for skin infection diseases by inhibiting S. aureus growth.

scholarly journals Pengujian Aktivitas Antibakteri Hidrogel Ekstrak Etanol Daun Tagetes erecta L

Jurnal MIPA ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 96
Author(s):  
Hosea Jaya Edy ◽  
Marchaban Marchaban ◽  
Subagus Wahyuono ◽  
Agung Endro Nugroho
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethanolic Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Tagetes Erecta ◽  
Tagetes Erecta L

Kandungan kimia flavonoid dan fenolik dalam ekstrak daun T.erecta diketahui memiliki aktivitas antibakteri yang baik. Pengunaan ekstrak daun T.erecta sebagai zat aktif sediaan hidrogel akan sangat mempermudah pemanfaatan dalam proses terapi oleh masyarakat. Tujuan dalam penelitian ini adalah melihat kemampuan aktivitas antibakteri sediaan hidrogel dengan zat aktif ekstrak daun T.erecta. Dalam penelitian ini digunakan 6 variasi konsentrasi ekstrak yang terkandung pada sediaan hidrogel yaitu 1.5; 2.0; 2.5; 3.0; 3.5; dan 4.0 %. Pengujian aktivitas antibakteri menggunakan metode difusi sumuran terhadap bakteri Staphylococcus aureus dan Escherichia coli. Aktivitas antibakteri terbesar ditunjukkan oleh sediaan hidrogel dengan konsentrasi ekstrak daun T.erecta 2.5%. Diameter zona hambat yang terbentuk terhadap pertumbuhan E.coli adalah 15.33 mm sedangkan diameter zona hambat terhadap S.aureus adalah 17.87 mm. Untuk kontrol positif digunakan bioplacenton® dan untuk kontrol negatif digunakan basis hidrogel tanpa kandungan ekstrak T.erectaFlavonoid and phenolic on ethanolic extract of T.erecta L has been antibacterial activity. The aim of this research was to determinate of antibacterial from hydrogel with ethanolic extract of T.erecta L. This research was begun making hydrogels with six variations of ethanolic extract of T.erecta L were 1.5; ; 2.0; 2.5; 3.0; 3.5; and 4.0 %.  Antibacterial activity used hole diffusion method against Staphylococcus aureus and Escherichia coli. Hydrogel containing 2.5 % ethanolic extract of T.erecta L was shown the highest antibacterial activity. The diameters of the inhibition zone against E.coli was 15.33 mm and against S.aureus was 17.87 mm. Positive control used bioplacenton® and negative control was hydrogel without ethanolic extract of T.erecta content

scholarly journals Daya bunuh dan daya hambat antimikrobial chlorhexidine 2% dan povidone iodine 1% sebagai medikamen saluran akar terhadap Enterococcus faecalis The ability of chlorhexidine 2% and povidone iodine 1% as root canal medicaments to kill and inhibit Enterococcus faecalis

2019 ◽  
Vol 7 (1) ◽  
pp. 12
Author(s):  
Nathania Astria ◽  
Ari Subiyanto ◽  
Latief Mooduto
Keyword(s):  
Enterococcus Faecalis ◽  
Root Canal ◽  
Povidone Iodine ◽  
Inhibition Zone ◽  
Colony Growth ◽  
Negative Control ◽  
Nutrient Agar ◽  
Diffusion Method ◽  
The Media ◽  
Agar Media

Background. Enterococcus faecalis is one of the pathogenic organisms associated with root canal treatment failure and apical periodontitis. Root canal medicament  is needed to prevent re-infection in the root canal and increase the success of treatment. Chlorhexidine and povidone iodine is a broad spectrum root canal medicaments that can kill gram-positive bacteria. Purpose. The purpose of this study was to discover the ability to kill and inhibit of antimicrobial chlorhexidine 2% and 1% povidone iodine asvroot canal medicaments against bacteria Enterococcus faecalis. Methode. This research was done by measuring the inhibition zone and count the number of colonies. Determination of the inhibition of root canal medicaments against Enterococcus faecalis by diffusion method. 10 microliter root canal medicaments dropped on paperdisk and placed on nutrient agar media with enterococcus faecalis, then inhibition zone was calculated. Determination ability to kill enterococcus faecalis is done by inserting 1 ml medicaments root canal into 5 ml BHIB media, then 0.05 ml inoculum of Enterococcus faecalis inserted into each tube, except the negative control. 0.1 ml of each tube implanted in the media nutrient agar. Media incubated for 24 hours, then Enterococcus faecalis bacterial colonies that grow in media calculated using the CFU. Results. There no colony growth of enterococcus faecalis in both root canal medicaments. There are significant differences in inhibition zone of 2% chlorhexidine and 1% povidone iodine (p<0.05). Conclusion. Both of root canal medicaments can kill enterococcus faecalis, but chlorhexidine 2% was more capable inhibit Enterococcus faecalis.

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