scholarly journals Alveolar Bone Osteoclast Profile in the Periodontitis Wistar Rats Model with the Snail Slime (Achatina Fulica) Application

2019 ◽  
Vol 7 (10) ◽  
pp. 1680-1684 ◽  
Author(s):  
I Gusti Agung Ayu Putu Swastini ◽  
Tjokorda Gde Bagus Mahadewa ◽  
I Putu Eka Widyadharma
Keyword(s):  
Wistar Rats ◽  
Alveolar Bone ◽  
Profile Analysis ◽  
Statistical Significance ◽  
Healing Process ◽  
Staining Procedure ◽  
Control Group ◽  
Osteoblast Activity ◽  
Post Hoc ◽  
One Way Anova

BACKGROUND: Bone damage is a result of periodontal disease that occurs due to changes in osteoclast and osteoblast activity in response to local inflammation. The bacteria Aggregatibacter actinomycetemcomitans produces Lipopolysaccharide (LPS), which can increase osteoclast activity. AIM: This study aimed to analyse the decrease in alveolar bone osteoclasts in periodontitis rats' model with the application of snail slime. METHODS: Wistar rats (27) with periodontitis divided into three groups, namely the control group (debridement), P1 group (debridement and application of oral snail slime) 300 Mg/Kg Body weight, P2 group (debridement, application of topical snail slime) 0.1 Mg. Osteoclast profile analysis was carried out by HE staining procedure to determine the histological feature of osteoclasts. The statistical significance was determined using the Shapiro-Wilk Test, One Way ANOVA, and Post Hoc test (p < 0.05). RESULTS: Osteoclast profile in rats with periodontitis applied with snail slime significantly decreased the number of osteoclasts with both oral and topical administration, there were significant differences in the number of osteoclasts between groups (one way ANOVA, p < 0.05) and there were no significant differences between groups P1 and P2 (Post Hoc, p > 0.05). CONCLUSION: In this study, there was a decrease in the number of osteoclasts which were slipped by snail slime in Wistar rats with periodontitis; this indicates a periodontitis healing process.

The Effects of Brotowali (Tinospora crispa) Extract on The Number of Macrophage Cells in Wistar Rats (Rattus norvegicus) Suffering from Periodontitis

2021 ◽  
pp. 3077-3081
Author(s):  
Tuti Kusumaningsih ◽  
Ira Arundina ◽  
Tantiana Tantiana ◽  
Rini Devijanti R ◽  
Indeswati Diyatri ◽  
...  
Keyword(s):  
Treatment Group ◽  
Wistar Rats ◽  
Healing Process ◽  
Periodontal Tissues ◽  
Macrophage Cells ◽  
Group I ◽  
Significant Difference ◽  
Histological Slide ◽  
Post Hoc ◽  
One Way Anova

Background: Periodontitis is an inflammatory disease of the periodontal tissues usually provoked by Actinobacillus actinomycetemcomitans (A. actinomycetemcomitans) bacteria. In the periodontitis healing process, macrophages play a significant role in the inflammatory stage. Brotowali (Tinospora crispa) consists of flavonoids that can stimulate periodontitis healing. Methods: Brotowali extract was derived from dried brotowali stems, processed into powder, and macerated with ethanol 80%. After that, the extraction was performed in three different concentrations, which were 25%, 50%, and 100%. five groups of Wistar rats were divided into Healthy wistar rats (control negative) Wistar rats suffering from periodontitis (control positive), Wistar rats suffering from periodontitis treated with 0.1 ml of 25% brotowali extract (treatment I), suffering from periodontitis treated with 0.1 ml of 50% brotowali extract (treatment II), and Wistar rats suffering from periodontitis treated with 0.1 ml of 100% brotowali extract (treatment III). The histological slide of each sample then was made to determine the number of macrophages cells. Results: One-Way ANOVA analysis revealed a notable difference among the groups. Tukey HSD Test (Post Hoc Test) showed no significant difference between (treatment group I) and (treatment group II). Conclusion: Brotowali extract may influence macrophage cells count of Wistar rats caused by A. actinomycetemcomitans bacteria.

scholarly journals Evaluation of osteogenic properties after application of hydroxyapatite-based shells of Portunus pelagicus

2021 ◽  
Vol 54 (3) ◽  
pp. 119
Author(s):  
Michael Josef Kridanto Kamadjaja ◽  
Alya Nisrina Sajidah Gatia ◽  
Agtadilla Novitananda ◽  
Lintang Maudina ◽  
Harry Laksono ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Tooth Extraction ◽  
Alveolar Bone ◽  
Control Group ◽  
Portunus Pelagicus ◽  
Treatment Groups ◽  
Significant Difference ◽  
Hydroxyapatite Gel ◽  
One Way Anova ◽  
Bone Dimensions

Background: After tooth extraction, the socket leaves a defect on the alveolar bone. The administration of shell crab-derived hydroxyapatite maintains bone dimensions that are important for achieving successful prosthodontic treatment. Purpose: The aim of the study was to determine the osteogenic properties, such as the number of osteoclasts, osteoblasts and osteocytes, after the application of hydroxyapatite-based shell crab in the post-extraction sockets of Wistar rats. Methods: There were two groups: the control group (K) and the treatment group (T). Wistar rats were randomly divided into control and treatment groups. After tooth extraction, hydroxyapatite gel derived from Portunus pelagicus shells was applied to the tooth sockets of Wistar rats. Observations and calculations of osteoclasts, osteoblasts and osteocytes were carried out on the 14th and 28th days under a light microscope with 400 times magnification. Statistical analysis was performed using one-way ANOVA. Results: There was a significant difference (p<0.05) between the K14 and P14 groups, K28 and P28 groups, K14 and K28 groups, and P14 and P28 groups. The results indicated that there were significant differences between groups of variables. Conclusion: The application of shell crab-derived hydroxyapatite (Portunus pelagicus) was able to decrease the number of osteoclasts and increase the number of osteoblasts and osteocytes.

Application of Hydroxyapatite scaffold from Portunus pelagicus on OPG and RANKL expression after tooth extraction of Cavia cobaya

2021 ◽  
pp. 4647-4651
Author(s):  
Michael Josef Kridanto Kamadjaja ◽  
Sherman Salim ◽  
Birgitta Dwitya Swastyayana Subiakto
Keyword(s):  
Tooth Extraction ◽  
Alveolar Bone ◽  
Control Group ◽  
Rankl Expression ◽  
Control Group Design ◽  
Portunus Pelagicus ◽  
Group Design ◽  
Post Test ◽  
Hydroxyapatite Scaffold ◽  
One Way Anova

Objective: This study was to determine OPG and RANKL expression after hydroxyapatite (HA) scaffold from crab shells (Portunus pelagicus) application in tooth socket of Cavia cobaya. Methods: This study was a post-test only control group design. Twenty four Cavia cobaya was divided into 4 groups. The lower left incisor was extracted and given a combination of HA and gelatin scaffold. Experimental animals were sacrificed on the 7th and 14th day. The amount of OPG and RANKL expression was calculated under a light microscope at 1000x magnification. The statistical analysis was done by One Way ANOVA Test and Tukey HSD. Results: Compared to other groups, the lowest and the highest level of OPG and RANKL were in P14 group. Conclusion: HA scaffold from crab shells (Portunus pelagicus) can increase OPG expression and decrease RANKL expression in the process of regenerating alveolar bone after tooth extraction.

scholarly journals Antimicrobial effect of methylene blue formulations with oxygen carrier at different pHs: preliminary study

2019 ◽  
Vol 22 (1) ◽  
pp. Process
Author(s):  
Jessica Klöckner Knorst ◽  
Gabriela Scarton Barriquello ◽  
Marcos Antônio Villetti ◽  
Roberto Christ Vianna Santos ◽  
Karla Zanini Kantorski
Keyword(s):  
Methylene Blue ◽  
Pseudomonas Aeruginosa ◽  
Photodynamic Therapy ◽  
Oxygen Carrier ◽  
Statistical Significance ◽  
Antimicrobial Effect ◽  
Control Group ◽  
Preliminary Study ◽  
Post Hoc ◽  
Multispecies Biofilms

Objective: Evaluate methylene blue (MB) formulations containing oxygen carrier at different pHs in antimicrobial photodynamic therapy (aPDT). Material and Methods: Biofilms of Pseudomonas aeruginosa PA01 formed over acrylics specimens during five days were treated with aPDT using different formulations: MB/pH 7.4; MB/pH 5.6; MB/carrier pH 7.4; MB/carrier pH 5.6. Biofilms not exposed to treatment were used as a control. Blind examiner for the experimental groups performed the counting of colonies per ml suspension (CFU/ml). Two-way ANOVA was used to determine the effect of factors solvent (carrier vs water) and pH (7.4 vs 5.6). One-way ANOVA and post-hoc Tukey’s test was used to evaluate differences among the five groups (control; MB/carrier pH 7.4; MB pH 7.4; MB/carrier pH 5.6; MB pH 5.6). The Statistics 8.0 software was used (P<0.05). Results: All of photodynamic therapy groups showed significant reduction in P. aeruginosa compared to the control group. The solvent factor was not significant (P=0.18), while the pH factor presented statistical significance (P=0.01). When the carrier was used, MB formulation at pH 7.4 presented a statistically greater reduction of P. aeruginosa than the formulation with pH 5.6. Conclusion: The PDT using methylene blue formulations with oxygen carrier demonstrated potential for the treatment of localized infections by P. aeruginosa. MB formulations with oxygen carrier and pH 7.4 resulted in higher antimicrobial effect and should be considered for future studies with multispecies biofilms.  KeywordsAntimicrobial photodynamic therapy; biofilm; laser; Pseudomonas aeruginosa.

scholarly journals PENGARUH PEMBERIAN GEL KOMBINASI EKSTRAK GETAH PEPAYA (Carica papaya L) DAN EKSTRAK DAGING LIDAH BUAYA (Aloe vera) TERHADAP PROSES PENYEMBUHAN ULKUS TRAUMATIKUS PADA MALE WISTAR RATS YANG MENDERITA DIABETES MELITUS

2015 ◽  
Vol 2 (1) ◽  
pp. 41
Author(s):  
Umi Kulsum ◽  
Ratnawati Hendari ◽  
Siti Chumaeroh
Keyword(s):  
Wound Healing ◽  
Ulcer Healing ◽  
Wistar Rats ◽  
Povidone Iodine ◽  
Healing Process ◽  
Aloe Vera ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Papaya Latex

Introduction: Research on the use of papaya and aloevera sap on wound healing each been done, but effects the combination of the two extracts on wound healing in diabetes mellitus condition has not been done.Purpose: This study aims to determine the effect of a gel combination of papaya latex and aloevera flesh extracts on the traumatics ulcer healing process in diabetic rats.Methods: True experimental laboratory research with the post-test-only control group design that performed on 28 male wistar rats which were divided intofour groups: DM and non-DM rat that treatment with povidone iodine and gel combination of papaya latex and aloevera flesh extract. The treatment was done for 7 days with smearing that was given 2x/day. Traumatics ulcer healing seen from the number of fibroblasts that then were analyzed by One WayAnova and post hoc LSD test.Results: The results showed the number of fibroblasts in DM and non-DM rat with povidone iodine were 44.6 and 42.73; in the DM and non-DM rat with a gel combination of papaya latex and aloe vera flesh extracts were 61.10 and 77.03. One Way Anova test resulted p-value = 0.000, it was mean that there were at least a significant difference on the number of fibroblasts in the two groups. Differences of the number fibroblasts was shown in all groups, except in the group DM and non-DM rats with povidone iodine (p = 0.764).Conclusion: Conclusion of the study was the provision of a gel combination of papaya latex and aloevera flesh extracts effect on the traumatics ulcer healing process in diabetic rats.

scholarly journals PENGARUH EKSTRAK DAGING LIDAH BUAYA (Aloe Vera) TERHADAP PENYEMBUHAN ULSERASI MUKOSA MULUT PADA MALE WISTAR RATS

2015 ◽  
Vol 1 (1) ◽  
pp. 25
Author(s):  
Laila Fitrotuz Zahroh ◽  
Rahmawati Sri Praptiningsih ◽  
Moh. Baehaqi
Keyword(s):  
Oral Mucosa ◽  
Wistar Rats ◽  
Healing Process ◽  
Research Result ◽  
Aloe Vera ◽  
Control Group ◽  
Control Group Design ◽  
Significant Difference ◽  
Male Wistar Rats

Background: Oral mucosa ulceration which often occurs usually in the form of white-yellowish spot with concave surface, reddish edge and pain. Based on previous research, Aloe vera process anti-inflammation substance that could help quickening ulceration healing process. This research aims to know the effect of Aloe vera flesh extract on Male wistar rats oral mucosa ulceration in-vivo. Method: this research was quasi experimental research with the post-test only control group design using Male wistar rats as the testing animal. In the research, there were three treatment groups: The first groups which was given aquadest treatment, second groups with Aloe vera flesh extract, and third groups which was given chlorhexidine gluconate 0,2% treatment. The data collecting was based on histopathology observation concerning the increase of fibroblast quantity. Result: The research result based on comparison test among the three groups with One Way Anova showed that on Day 3th, the average quantity of fibroblast didn't have significant difference between the treatment group and control group positive that was p>0,05, meanwhile on Day 7th every group showed significant difference p<0,05. Conclusion: It concluded that Aloe vera flesh extract has influence on the healing of Male wistar rats oral mucosa ulceration as shown by fibroblast increasing quantity.

scholarly journals EFEK ANTIBAKTERI EKSTRAK DAUN MIMBA (Azadirachta indica) TERHADAP PERTUMBUHAN Enterococcus faecalis SECARA IN-VITRO

2019 ◽  
Vol 11 (1) ◽  
pp. 23-32
Author(s):  
Cut Soraya ◽  
Sunnati ◽  
Fenny Wulandari
Keyword(s):  
Phenolic Compound ◽  
Enterococcus Faecalis ◽  
Azadirachta Indica ◽  
Control Group ◽  
Post Hoc ◽  
One Way Anova ◽  
Banda Aceh

Perawatan saluran akar adalah perawatan yang dilakukan untuk mempertahankan gigi agar dapatberfungsi di lengkung gigi selama mungkin. Penyebab dominan dari kegagalan perawatan endodontikadalah adanya bakteri di dalam saluran akar. Bakteri yang paling sering ditemukan pada isolasi darigigi yang mengalami kegagalan perawatan saluran akar adalah Enterococcus faecalis (E.faecalis).Salah satu jenis tanaman herbal yang memiliki sifat antibakteri yang tinggi adalah tanaman mimba(A.indica). Tujuan penelitian ini untuk menganalisis efek antibakteri ekstrak daun mimba (A.indica)terhadap pertumbuhan E. faecalis. Penelitian ini merupakan penelitian eksperimental laboratorisdengan desain penelitian true experimental posttest only control group. Penelitian dilalukan denganmenguji ekstrak daun mimba (A.indica) pada konsentrasi 10%, 20%, 40%, 60%, 80% dan 100%terhadap pertumbuhan E. faecalis yang dilakukan di Laboratorium Mikrobiologi Fakultas KedokteranHewan (FKH) Universitas Syiah Kuala Banda Aceh. Hasil uji fitokimia yang dilakukan menunjukkanbahwa ekstrak daun mimba (A. indica) positif mengandung triterpenoid, phenolic compound, tanin,steroid dan saponin yang berperan sebagai antibakteri. Hasil uji efek antibakteri ekstrak daun mimba(A. indica) menunjukkan adanya pembentukan zona hambat di sekitar kertas cakram pada setiapkonsentrasi. Hal ini membuktikan bahwa ekstrak daun mimba memiliki efek antibakteri yangmemiliki kemampuan dalam menghambat pertumbuhan E. faecalis. Uji statistik digunakanmenggunakan one way ANOVA. Kemudian dilakukan uji lanjut (Post hoc test) menggunakan LeastSignificant Difference untuk menganalisis perbedaan efek antibakteri ekstrak daun mimba (A. indica)dalam konsentrasi 10%, 20%, 40%, 60%, 80% dan 100% terhadap pertumbuhan E.faecalis.Kesimpulan penelitian ini ekstrak daun mimba (A. indica) berpotensi menghambatpertumbuhan E. faecalis.Kata Kunci: Azadirachta indica, Enterococcus faecalis, perawatan saluran akar.

scholarly journals THE COMPARISON OF RAMANIA (Bouea macrophylla Griff) AND BINJAI (Mangifera caesia) LEAVES EXTRACT GEL EFFECT ON COLLAGEN DENSITY

2021 ◽  
Vol 6 (2) ◽  
pp. 159
Author(s):  
Nabilah Nabilah ◽  
Irham Taufiqurrahman ◽  
Nolista Indah Rasyid
Keyword(s):  
Wistar Rats ◽  
Leaf Extract ◽  
Total Sample ◽  
Control Group ◽  
Control Group Design ◽  
Gel Effect ◽  
Index Measurement ◽  
Male Wistar Rats ◽  
Collagen Density ◽  
Post Hoc

Background: The extract of ramania Bouea macrophylla Griff) and binjai (Mangifera caesia) leaf have flavonoid compounds that function as antioxidants to balance the amount of Reactive Oxygen Species (ROS) in tissues and optimize wound healing by helping synthesis of hydroxyproline which is used as a collagen synthesis material. Objective: To compare the effect of 15% concentration of ramania leaf extract gel, 15% concentration of binjai leaf extract gel on collagen density in back incision wounds of male Wistar rats on day 7 and day 14. Methods: This study used a true experimental design with a posttest-only with control group design. The study sample used male wistar rats that were healthy and active, aged 2-3 months with a body weight of 250-300 grams. The total sample was 18 rats divided into 6 groups. The 15% concentration of ramania and binjai leaves was given topically, then the rats were euthanized on the 7th and 14th day. Collagen index measurement was using hydroxyproline concentration. Results: Two-way Anova data analysis showed a significant value of 0.00 (p<0.05), which means that there was a difference in effect between the treatment gel and wound day. Bonferroni Post Hoc test showed a significant value in all treatment gel groups. Conclusion: There is a difference in the effect of 15% concentration of ramania leaf extract gel and 15% concentration of binjai extract gel on collagen density. Binjai leaf extract gel at 15% concentration is more effective for collagen density than Ramania leaf extract at 15% concentration and placebo. Keywords : Binjai Leaf Extract Gel, Collagen, Hydroxyproline, Ramania Leaf Extract Gel.

scholarly journals The effect of vitamin C on Endosulfan-induced oxidative stress parameters in prepubertal rats

Biomedicine ◽  
2020 ◽  
Vol 39 (2) ◽  
pp. 333-338
Author(s):  
Kalaivani Manokaran ◽  
Vasanthalaxmi Krishnananda Rao ◽  
Nilima . ◽  
Manjula Shimoga Durgoji Rao ◽  
Sucheta Prasanna Kumar
Keyword(s):  
Oxidative Stress ◽  
Vitamin C ◽  
Wistar Rats ◽  
Statistical Significance ◽  
Treated Group ◽  
Reproductive Organs ◽  
Protective Role ◽  
Control Group ◽  
Group I ◽  
Testicular Weight

Introduction and Aim: Oxidative stress plays a very important role in endosulfan-induced toxic effects on reproductive organs. Vitamin C is a potent antioxidant which plays an important role in decreasing oxidative stress. The present study was aimed to investigate the protective role of vitamin C against endosulfan-induced testicular toxicity in Wistar rats. To investigate a protective effect of vitamin C against endosulfan induced toxicity on biochemical changes. Materials and Methods: Seventy male neonatal Wistar rats were divided into  seven groups. The group  I was taken as the control group, the endosulfan-treated were grouped into II (3 mg/kg body weight (BW) and group III (6 mg/kg BW), Group IV (9 mg/kg BW) and Group V (12 mg/kg BW). Group VI (9 mg/kg BW) and group VII (12 mg/kg BW) were pretreated with vitamin C (20 mg/kg BW) for 60 days. After  the experimental procedures, the testicular weight, lactate dehydrogenase (LDH) enzyme and testosterone in plasma, LDH, steroidogenic enzymes 3?-HSD and 17?-HSD in testis were evaluated. One-way ANOVA was used to determine the statistical significance. Results: Significant improvement in the testicular weight (P<0.05) , LDH (P<0.05) levels both in plasma and testis, increase in testosterone(P<0.001) and steroidogenic enzyme levels(P<0.001) was observed in the group pretreated with vitamin C treated group when compared to the endosulfan treated group. Conclusion: Vitamin C decreases the toxic effect of endosulfan on testis. The present action might be  due to its antioxidative properties.

scholarly journals Pengaruh Senam Nifas terhadap Penurunan Tinggi Fundus Uteri dan Lochea pada Ibu Pasca Bersalin yang Mendapatkan Inisiasi Menyusu Dini dan Mobilisasi Dini

2020 ◽  
Vol 19 (03) ◽  
pp. 126-133
Author(s):  
Siska Toloan Toloan ◽  
Harimat Hendarwan
Keyword(s):  
Control Group ◽  
P Value ◽  
Control Group Design ◽  
Group Design ◽  
Consecutive Sampling ◽  
Post Test ◽  
Post Hoc ◽  
One Way Anova

Masa nifas adalah hal sangat penting untuk diperhatikan guna untuk menurunkan angka kematian ibu dan bayi di Indonesia. Penelitian ini bertujuan untuk Mengetahui Pengaruh Senam Nifas Terhadap Penurunan Tinggi Fundus Uteri Dan Lochea Pada Ibu Pasca Bersalin Yang Mendapatkan Inisiasi Menyusu Dini Dan Mobilisasi Dini Di Praktek Bidan Mandiri Kota Depok Tahun 2019. Penelitian ini menggunakan penelitian kuantitatif yang menggunakan desain penelitian Quasy Exsperimental dengan pendekatan post test only control group design. Populasi yang diambil yaitu 65 ibu pasca bersalin. Sampel diambil dengan metode consecutive sampling besar sampel ditentukan dengan menggunakan rumus Federer setiap variabel bebas terdiri atas 3 kelompok dan setiap kelompok sebanyak 9 sampel sehingga jumlah total sampel 27 ibu pasca bersalin. Pengumpulan data berupa data primer dan uji yang digunakan yaitu uji One Way Anova. Dapat dilihat dari nilai signifikan uji One Way Anova pada penurunan tinggi fundus uteri hari pertama sampai hari kesepuluh dengan nilai signifikan yaitu p-value < 0,05 yang artinya H0 ditolak atau ada perbedaan secara signifikan dan dilihat. Hasil post hoc test tidak terdapat perbedaan signifikan pada kelompok inisiasi menyusu dini, inisiasi menyusu dini dan mobilisasi dini dan kelompok inisiasi menyusu dini, mobilisasi dini dan senam nifas terhadap penurunan tinggi fundus uteri.

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