Orthogonal Test for Optimization of Total Saponin of Gynostemma pentaphyllum Extraction by Decompressing Inner Ebullition

2014 ◽  
Vol 608-609 ◽  
pp. 1128-1130
Author(s):  
Yan Ying Weng ◽  
Guang Kuang Deng
Keyword(s):  
Volume Fraction ◽  
Raw Materials ◽  
Extraction Process ◽  
Extraction Yield ◽  
Ethanol Solution ◽  
Orthogonal Test ◽  
Extraction Temperature ◽  
Extraction Technology ◽  
Gynostemma Pentaphyllum ◽  
Total Saponin

In order to optimize the extraction technology of total Saponin of Gynostemma pentaphyllum by decompressing innner ebullition, the technology was established between the extraction yield of total Saponin and several independent variables (the concentration of desorption agent, solid-liquid ratio, extraction temperature and time) through orthogonal test ,which was based on the single factor experiment. Experiment results show that the optimum extraction conditions for 5.0 g raw materials are as followed: the volume fraction of ethanol solution saturated the materials is 60% , the volume of water is 19 times to material. The extraction process can finish after 5 min at 50 °C twice. The results indicate that orthogonal test design can be an effective method to optimize the extraction technology of total Saponin of Gynostemma pentaphyllum through decompressing innner ebullition.

Extraction Process of Amaranthine Pigment Keeping the Water Wax Fuit Biological Active

2013 ◽  
Vol 295-298 ◽  
pp. 150-154
Author(s):  
Feng Jie Wang ◽  
Xin Qiao Dong ◽  
Xiao Liu
Keyword(s):  
Citric Acid ◽  
Extraction Method ◽  
Raw Materials ◽  
Extraction Process ◽  
Extraction Yield ◽  
Extraction Temperature ◽  
Natural Pigment ◽  
Pigment Extraction ◽  
Acid Aqueous Solution ◽  
The Stability

Development natural pigment retained a variety of nutrition composition is extremely research value.Water Wax Trees Fruit was as raw materials,citric acid aqueous solution was as extracting agent.Amaranthine pigment extraction method were studied on fully grasping the stability of the natural amaranthine pigment and no damage to the water wax fruit biological activity.The effect of extraction yield were studied on Water Wax Trees Fruit natural amaranthine pigment.The results show that the better extraction conditions are extraction temperature at 70 °C, extraction time for 3h,citric acid concentration1.0 % , the material/liquid ration(g/ml) 1:5.The extraction yield is 2.95%.Ultraviolet absorption maximum wavelength is 280 nm.

Optimization of the Ultrasonic Extraction of Gypenoside III from Gynostemma pentaphyllum by Response Surface Methodology

2012 ◽  
Vol 550-553 ◽  
pp. 1866-1870
Author(s):  
Xiao Dan Tang ◽  
Hai Yang Hang ◽  
Shao Yan Wang ◽  
Jing Xiang Cong
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Extraction Yield ◽  
Raw Material ◽  
Extraction Temperature ◽  
Optimal Conditions ◽  
Gynostemma Pentaphyllum ◽  
Bioactive Component ◽  
Yield Value ◽  
Ultrasonic Time

Gypenosides III is a major bioactive component which is rich in Gynostemma pentaphyllum. For better utilization of the native resource, response surface methodology was used to optimize the extraction conditions of gypenosides III from G. pentaphyllum. The effects of three independent variables on the extraction yield of gypenosides III were investigated and the optimal conditions were evaluated by means of Box-Behnken design. The optimal conditions are as follows: ratio of ethanol to raw material 25, extraction temperature 58°C and ultrasonic time 25min. Under these conditions, the yield of gypenoside III is 1.216±0.05%, which is agreed closely with the predicted yield value.

Optimization of Extraction Conditions of Essential Oil from Latifolia miq Roots and Stems with Supercritical CO2 Fluid

2013 ◽  
Vol 726-731 ◽  
pp. 4512-4516
Author(s):  
Ren Yong Gu ◽  
Zhi Ping Li
Keyword(s):  
Essential Oil ◽  
Optimum Condition ◽  
Raw Materials ◽  
Extraction Process ◽  
Orthogonal Test ◽  
Single Factor ◽  
Extraction Condition ◽  
Optimum Extraction ◽  
Optimum Extraction Condition

Studying on the extraction process of essential oil from Latifolia Miq roots and stems by Supercritical CO2 extraction. The effects on extracting ratio were investigated by using single factor test, adding content of grinding fineness of raw materials,extracting temperature, extracting pressure and extracting time. The optimum extraction condition was determined by orthogonal test. The results showed that the optimum condition of the extraction was as follows: grinding fineness 90 mesh, extracting temperature 45 °C, extracting pressure 20 MPa and extracting time 2.5 h.

Study on Extraction Technology and Stability of Melanin from Banana Peel

2013 ◽  
Vol 815 ◽  
pp. 287-292
Author(s):  
Li Jing Min ◽  
Jing Fen Li
Keyword(s):  
Metal Ions ◽  
Light Source ◽  
Room Temperature ◽  
Ph Value ◽  
Orthogonal Test ◽  
Raw Material ◽  
Banana Peel ◽  
Extraction Temperature ◽  
Solution Ph ◽  
Extraction Technology

[Objectiv The aim is to study the extraction technology of melanin from banana peel, and discuss its stability. [Metho We used banana peel as raw material, the absorbance at 410 nm as the index, extracted melanin by base-acid method, and optimized the extraction technology by the single factor and orthogonal test. Then we discussed the melanins stability at different temperature, light source, pH value, metal ions, antioxidant and reducing agents.[Result The best extraction conditions of melanin is that, hydrochloric acid soak time 5 h, alkaline solution pH value 14, extracting time 20 min, extraction temperature 70 °C, absorbance value about 0.309 A. Melanin from banana peel is stable at high temperature, different light source irradiation, reducing agent coexist; but at room temperature, metal ions and oxidizing agent coexist, it is unstable. [Conclusio This study provided experimental basis for the optimization and development of melanin from banana peel.

scholarly journals Optimization of High-Pressure Extraction Process of Antioxidant Compounds from Feteasca regala Leaves Using Response Surface Methodology

Molecules ◽  
2020 ◽  
Vol 25 (18) ◽  
pp. 4209
Author(s):  
Anca Becze ◽  
Vanda Liliana Babalau-Fuss ◽  
Cerasel Varaticeanu ◽  
Cecilia Roman
Keyword(s):  
Response Surface Methodology ◽  
Antioxidant Capacity ◽  
Response Surface ◽  
Total Antioxidant Capacity ◽  
Raw Materials ◽  
Extraction Process ◽  
Extraction Yield ◽  
Antioxidant Compounds ◽  
Operational Conditions ◽  
Total Antioxidant

Circular economy principles are based on the use of by-products from one operation as the raw materials in another. The aim of this work is to obtain extracts with high antioxidant capacity and resveratrol content for the superior capitalization of the biomass of Feteasca regala leaves obtained during vineyard horticultural operations in spring. In order to obtain a high-quality extract at an industrial level, an optimal extraction process is needed. Central composite design (CCD) was used for the experiment design, which contained three independent variables: the ratio of extraction solvent to solid matter, temperature (°C) and time (minutes). The evaluation of extracts was done by measuring the total antioxidant capacity of the extracts using photo-chemiluminescent techniques, and the resveratrol content using liquid chromatography. Process optimization was done using response surface methodology (RSM). Minitab software version 17.0 was used for the design of experiments and data analysis. Regression analysis showed that the model predicts 87.5% of the variation for resveratrol and 96% for total antioxidant capacity (TAC). The temperature had the biggest influence on the extraction yield. The optimal operational conditions for the extraction method applied had the following conditions: ratio e/m 2.92; 43.23 °C and 55.4 min. A maximum value of 34,623 µg ascorbic acid equivalent (AAE) /mL total antioxidant capacity and 182.4 µg/mL resveratrol content were obtained when the optimal extraction parameters where used. The values obtained in experiments proved that by using RSM an accurate model can be obtained for extraction of Feteasca regala leaves.

scholarly journals Determining the Optimum Conditions for Extracting Carotenoids from Syrian Apricot Fruits

2021 ◽  
pp. 11-20
Author(s):  
Wahbi Kalook ◽  
Adib Faleh ◽  
Amir Al-Haj Sakur ◽  
Wassim Abdelwahed
Keyword(s):  
High Performance Liquid Chromatography ◽  
High Performance ◽  
Extraction Process ◽  
Extraction Methods ◽  
Extraction Yield ◽  
Extraction Temperature ◽  
Optimum Temperature ◽  
Optimum Conditions ◽  
Total Carotenoids ◽  
Β Carotene

The aim of this research is to extract carotenoids from apricot fruits using a food solvent (ethanol) and a non-food solvent (propanol). In addition, it aims to study the effect of different extraction conditions, i.e., the extraction temperature (20-40-60)°C and the extraction times (4-8-12) hours, on the carotenoids yield in order to improve and develop extraction methods. The extracted carotenoids were determined using high-performance liquid chromatography (HPLC), and the studied carotenoids are α-carotene and β-carotene. It was found that β-carotene constitutes about 80% of the total carotenoids in apricots. The results indicated that the extraction yield using ethanol was low compared to the extraction yield using propanol. Propanol is the most suitable solvent for carotenoids extraction in comparison with ethanol. The temperature also had a significant effect on the extraction yield; at 20°C the extraction yield was very low, and 60°C was the optimum temperature for the extraction of carotene. The extraction yield significantly increased with time in the first hours of extraction, and there was no significant effect from increasing the extraction time for a period of 6-8 hours. Moreover, the pretreatment of fruits by freezing accelerated the extraction process and increased the extraction yield. The optimum conditions for extracting carotenoids were determined in the conditions of food extraction; the optimum conditions for extracting carotenoids are: extraction at 60°C for three hours with pretreatment by freezing as the extraction rate reached up to 6.36 mg/100 g using ethanol as a food solvent.

scholarly journals Research on the Extraction Process in Aqueous Two-Phase System of Moringa Leaf Flavonoids

2021 ◽  
Vol 267 ◽  
pp. 02010
Author(s):  
Rui Wang ◽  
Xueqin Zhang ◽  
Qian Zhou
Keyword(s):  
Raw Materials ◽  
Extraction Process ◽  
Ultrasonic Extraction ◽  
Extraction Time ◽  
Phase System ◽  
Extraction Temperature ◽  
Liquid Ratio ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Solid Liquid

Moringa leaves were used as raw materials, ethanol and ammonium sulfate were used to construct a aqueous two-phase system to extract the flavonoids from Moringa leaves. The solid-liquid ratio, ethanol content, ultrasonic extraction time, and ultrasonic extraction temperature were selected as single factors, and the extraction process of flavonoids was optimized through single-factor experiments and orthogonal experiments. The optimal process was: 1:110 of solid-liquid ratio, 45 % of ethanol concentration, 15 minutes of ultrasonic extraction time, 70 °C of ultrasonic extraction temperature. Under this condition, the extraction rate of Moringa leaf flavonoids reached 8.37 %.

Effect of Extraction Process on Extraction Yeild, Total Polyphenol Content and Antioxidant Activity of Jasminum Subtriplinerve

2019 ◽  
Vol 814 ◽  
pp. 527-531
Author(s):  
Trieu Tuan Anh ◽  
Luu Tu Hao ◽  
Long Giang Bach ◽  
Duy Chinh Nguyen ◽  
Tri Duc Lam
Keyword(s):  
Antioxidant Activity ◽  
Extraction Process ◽  
Extraction Yield ◽  
Total Phenolic ◽  
Extraction Temperature ◽  
Total Phenolic Compounds ◽  
Polyphenol Content ◽  
Total Polyphenol ◽  
Herbal Plants ◽  
Optimized Conditions

Jasminum Subtriplinerve Blume (Oleaceae) is herbal plants widely a tea for weight loss, stimulates milk glands. Extract product it easy used yet had little research. This study was carried out to evaluate the effect of extracting conditions such as water/material ratio, extraction temperature and extracting time on extracting yield, polyphenol content and antioxidant activity of J.subtriplinerve extract. The total phenolic compounds were determined according to the Folin–Ciocalteu method. Extraction yield (0.22g/100 g) was obtained at water/material ratio 15:1 (ml/g), 40°C and 4 hours extraction time. Under these optimized conditions, the polyphenol content of was 2640.4 μg/1g extract. And the extract can harvest 46.11 % of DPPH (30 μg/ mL) at 10 000 μg/ml concentration

Extraction and Analysis of Flaxseed Oil by Supercritical CO2 Method Optimized by RSM and GC-MS

2018 ◽  
Vol 17 (1) ◽  
pp. 11-16
Author(s):  
Mo Liu ◽  
Mengxi Wang ◽  
Dongya Chen ◽  
Lingyan Kong ◽  
Mei Xue ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Mass Spectroscopy ◽  
Food Production ◽  
Flaxseed Oil ◽  
Extraction Yield ◽  
Extraction Temperature ◽  
Extraction Technology ◽  
Effective Technique ◽  
Satisfactory Method ◽  
Gas Chromatography Mass Spectroscopy

Flaxseed oil has already been widely used in food production in recent years as of its prominent biological functions. This study was aimed to obtain high-quality flaxseed oil and find the suitable extraction parameters, exploiting response surface methodology of a three-variable, three-level Box-Behnken experimental design. In this study, supercritical CO2 extraction technology was used to extract flaxseed oil. The chemical composition of flaxseed oil was investigated by Gas Chromatography-Mass Spectroscopy. Results showed that the optimal conditions were extraction time of 4.58 h, extraction temperature at 51°C, and extraction pressure at 31 MPa. Under these conditions, the highest extraction yield of flaxseed oil was 23.95%. 9, 12, 15-Octadecatrienoic acid was indicated to be the highest content according to GC-MS analysis, with the content of 75.22%. In conclusion, supercritical CO2 extraction was proved to be an effective technique for extracting oil from flaxseed, and Gas Chromatography-Mass Spectroscopy provided the satisfactory method of analyzing flaxseed oil from flaxseed for industrial need.

Optimisation of the Ethanol-Based Heat Reflux Extraction of Isoflavones from Pueraria lobata (Willd.) Ohwi Root

2014 ◽  
Vol 955-959 ◽  
pp. 180-186 ◽  
Author(s):  
Shu Ya Wang ◽  
Jun Gao ◽  
Yi Lu Chen ◽  
Jun Zhou ◽  
Xiao Yu Yong ◽  
...  
Keyword(s):  
Large Scale ◽  
Ethanol Concentration ◽  
Orthogonal Experiment ◽  
Extraction Yield ◽  
Pueraria Lobata ◽  
Scale Production ◽  
Extraction Temperature ◽  
Extraction Technology ◽  
Large Scale Production ◽  
Solid Liquid

Ethanol-based heat reflux extraction has been successfully applied in extracting isoflavone compounds from the dried root of Pueraria lobata (Willd.) Ohwi. The different extraction factors including ethanol concentration, extraction temperature, extraction time, ratio of solid/liquid and number of extraction cycles has been studied on the influence of the extraction yield of isoflavone compounds. And the orthogonal experiment has been used to optimize the extracting conditions. A variety of indicators were employed to evaluate the extraction efficiency, including the yield of puerarin, daidzein and total isoflavone. The optimal extraction technology was as follows: extraction temperature 90°C, ratio of solid/liquid 1:6, ethanol concentration 90% and extraction 2 h. Under the optimal conditions extraction rates of puerarin, daidzein and total isoflavone were 37.51±0.64 mg/g, 2.63±0.03 mg/g and 117.4±0.89 mg/g. The study of this article can provide theoretical basis for large-scale production of puerarin, daidzein and total isoflavone.

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