Improvement of Hydroxyapatite Deposition on Titanium Dental Implant Using ArF Laser Ablation: Effect on Osteoblast Biocompatibility In Vitro

2006 ◽  
Vol 49 ◽  
pp. 282-289 ◽  
Author(s):  
Yoshiya Hashimoto ◽  
Masanobu Kusunoki ◽  
Ryota Hatanaka ◽  
Keiko Hamano ◽  
Hiroaki Nishikawa ◽  
...  
Keyword(s):  
Laser Ablation ◽  
X Ray Diffraction ◽  
In Vitro Biocompatibility ◽  
Primary Mouse ◽  
Significant Difference ◽  
Titanium Dental Implant ◽  
Bare Titanium ◽  
Mouse Osteoblast ◽  
Surface Conformation

To develop a better surface conformation of titanium dental implants, we examined the in vitro biocompatibility of a thin natural apatite (NA) film deposited by laser ablation. Thin (2000-Å) hydroxyapatite (HA) and NA films were deposited on titanium discs using an ArF excimer laser operating at a repletion rate of 10 Hz and annealed by heating at 360°C for 1 h. Energy dispersive analysis of the NA film revealed peaks of Na and Mg in addition Ca and P. X-ray diffraction showed that crystalline HA was present in the HA and NA films. Primary mouse osteoblast grew faster and had higher alkaline phosphatase activity when grown in NA films than on HA films or a bare titanium surface. In addition, osteocalsin production by these cells was higher on HA and NA than bare titanium, but there was no significant difference between cells grown on HA and NA. Thus surface modification with NA film may contribute to successful osteoblast function and differentiation at titanium interface.

scholarly journals A GREEN APPROACH FOR THE SYNTHESIS OF DRUG DELIVERY SYSTEM, MESOPOROUS SILICA GRAFTED ACRYLAMIDE –β- CYCLODEXTRIN COMPOSITE, FOR THE CONTROLLED RELEASE OF CURCUMIN

2018 ◽  
Vol 11 (9) ◽  
pp. 372
Author(s):  
Manohar D Mullassery ◽  
Noeline B Fernandez ◽  
Surya R ◽  
Diana Thomas
Keyword(s):  
Drug Delivery ◽  
Controlled Delivery ◽  
X Ray Diffraction ◽  
In Vitro Biocompatibility ◽  
Green Approach ◽  
Solvent Free Conditions ◽  
Good Biocompatibility ◽  
Biocompatibility Study

Objective: The scope of the present study was the preparation and characterization of a novel composite acrylamide β-cyclodextrin grafted 3-aminopropyltriethoxysilane bentonite (AMCD-g-APSB), for the controlled delivery of curcumin (CUR).Methods: AMCD-g-APSB, was synthesized by solvent-free conditions using microwave irradiation. The structure and surface morphology of the composite was established using Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, thermal analysis, etc.Results: The swelling percentage of the composite depends on both time and pH of the medium. The maximum swelling of the composite occurred at a pH of 7.4. The maximum drug encapsulation was occurring at a pH 3. About 96.5% of drug was loaded at pH 3. In vitro biocompatibility study was performed, and the result showed good biocompatibility of the composite in the concentration range 2.5–50 μg/ml.Conclusions: Drug delivery study of the composite proved that CUR could be successfully released in a controlled manner in the colon without much loses of the drug in the stomach.

scholarly journals Incorporation of Lippia citriodora Microwave Extract into Total-Green Biogelatin-Phospholipid Vesicles to Improve Its Antioxidant Activity

Nanomaterials ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 765
Author(s):  
Francisco Javier Leyva-Jiménez ◽  
Maria Letizia Manca ◽  
Maria Manconi ◽  
Carla Caddeo ◽  
José Antonio Vázquez ◽  
...  
Keyword(s):  
High Performance ◽  
Bioactive Molecules ◽  
Innovative Technology ◽  
Phospholipid Vesicles ◽  
Thunnus Albacares ◽  
Bioactive Substances ◽  
Lippia Citriodora ◽  
In Vitro Biocompatibility ◽  
Primary Mouse

Phytochemicals from Lippia citriodora leaves were extracted by applying an innovative technology based on the use of microwaves, which represents an alternative method to extract bioactive substances. The obtained extract was incorporated into phospholipid vesicles in order to promote the antioxidant effect of the bioactive molecules present in L. citriodora extract. The extract was analyzed by High Performance Liquid Chromatography coupled to Time-Of-Flight mass spectrometer by electrospray (HPLC-ESI-TOF-MS) and different phytochemicals were detected and quantified. The whole extract was incorporated in liposomes, glycerosomes (liposomes modified with glycerol) and propylene glycol-containing vesicles (PG-PEVs). Moreover, a biopolymer obtained from fish by-product, that is Thunnus albacares skin, was added to improve the bioactivity of the formulations. The in vitro biocompatibility and the antioxidant efficacy of the extract in solution or loaded in the vesicles were tested in primary mouse embryonic fibroblasts (3T3). The results showed the superior bioactivity of the vesicle formulations over the aqueous solution of the extract, which points to an interesting strategy for the treatment of skin disorders.

Investigation of PLA-PEG-45S5 Bioactive Glass Composite Enhancement on Bioactivity

2020 ◽  
Vol 833 ◽  
pp. 214-219
Author(s):  
Nik Syahirah Aliaa Nik Sharifulden ◽  
Siti Noor Fazliah Mohd Noor ◽  
Siti Fatimah Samsurrijal ◽  
Siti Nur Liyana Ramlee ◽  
Nur Syazana Azizan
Keyword(s):  
Thermal Analysis ◽  
Bioactive Glass ◽  
Sol Gel ◽  
X Ray Diffraction ◽  
Degradation Study ◽  
Significant Difference ◽  
Biomaterial Science ◽  
Materials Used ◽  
Hdf Cells

Bioactivity is an important aspect in biomaterial science ensuring materials used are safe for clinical application. The study describes fabrication of composites containing polylactic acid (PLA) – polyethylene glycol (PEG) with incorporation of sol-gel derived 45S5 bioactive glass (BG). Thermal analysis via Differential Thermal Analysis shows a favorable point over degree of crystallization that influence cells attachment, although non-significant difference in values indicates BG has homogenously dispersed. This correlates to X-ray diffraction analysis where non-significant difference is seen in intensities of the diffraction peaks, which confirms low impact of BG brittleness properties over the fabricated composite. Composites’ pH and degradation study in Simulated Body Fluid shows a steady increment profile over time and lower degradation rate for the composite after incorporation of BG. In vitro cell proliferation study also showed that HDF cells seeded on composite film of P/BG2.5 exhibit highest cell viability with steady increment of proliferation throughout the observation period.

Titanium Oxide (TiO2) Coatings Produced on Titanium by Oxygen-Plasma Immersion and Cell Behaviour on TiO2

2006 ◽  
Vol 309-311 ◽  
pp. 367-370 ◽  
Author(s):  
E.T. Uzumaki ◽  
A.R. Santos ◽  
C.S. Lambert
Keyword(s):  
Titanium Oxide ◽  
Oxygen Plasma ◽  
Three Dimensional ◽  
Titanium Substrate ◽  
X Ray Diffraction ◽  
Cell Behaviour ◽  
In Vitro Biocompatibility ◽  
Metallic Implants ◽  
Titanium Substrates

Plasma immersion process was investigated as a method for producing bioceramics coatings on metallic implants due to its advantages, which include the production of coatings on three-dimensional workpieces, with high density and superior adhesion. In this process, the oxygen plasma was utilized to form titanium oxide on titanium substrate. The structure, composition and surface morphology were studied using scanning electron microscopy (SEM) and X-ray diffraction. In addition a preliminary study has also been carried out, on TiO2-coated and uncoated titanium substrates, to analyse the in vitro biocompatibility (cytotoxicity evaluation and cell morphology).

scholarly journals Preparation and Biocompatibility of Poly Methyl Methacrylate (PMMA)-Mesoporous Bioactive Glass (MBG) Composite Scaffolds

Gels ◽  
2021 ◽  
Vol 7 (4) ◽  
pp. 180
Author(s):  
Irina Atkinson ◽  
Ana Maria Seciu-Grama ◽  
Oana Catalina Mocioiu ◽  
Ana Maria Mocioiu ◽  
Luminita Predoana ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Composite Scaffold ◽  
Bone Diseases ◽  
X Ray Diffraction ◽  
Composite Scaffolds ◽  
X Ray ◽  
In Vitro Biocompatibility ◽  
Mesoporous Bioactive Glass ◽  
Scanning Electron

In recent years, the rising number of bone diseases which affect millions of people worldwide has led to an increased demand for materials with restoring and augmentation properties that can be used in therapies for bone pathologies. In this work, PMMA- MBG composite scaffolds containing ceria (0, 1, 3 mol%) were obtained by the phase separation method. The obtained composite scaffolds were characterized by X-ray diffraction, infrared spectroscopy, and scanning electron microscopy. UV–Vis measurement and EDX analysis confirmed the presence of cerium ions in the composite scaffolds. Evaluation of the in-vitro biocompatibility using MTT assay showed that composite scaffold containing 1 mol% of ceria presented higher viability than control cells (100%) for concentrations ranging between 5 and 50% after 96 h of incubation.

scholarly journals Synthesis and Characterization of a Novel Biocompatible Alloy, Ti-Nb-Zr-Ta-Sn

2021 ◽  
Vol 22 (19) ◽  
pp. 10611
Author(s):  
Yuliya Y. Khrunyk ◽  
Sabrina Ehnert ◽  
Stella V. Grib ◽  
Anatoly G. Illarionov ◽  
Stepan I. Stepanov ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Elastic Modulus ◽  
Inflammatory Responses ◽  
X Ray Diffraction ◽  
In Vitro Biocompatibility ◽  
Transmission Electron ◽  
Low Elastic Modulus ◽  
O2 Plasma Treatment ◽  
Primary Human Osteoblasts

Many current-generation biomedical implants are fabricated from the Ti-6Al-4V alloy because it has many attractive properties, such as low density and biocompatibility. However, the elastic modulus of this alloy is much larger than that of the surrounding bone, leading to bone resorption and, eventually, implant failure. In the present study, we synthesized and performed a detailed analysis of a novel low elastic modulus Ti-based alloy (Ti-28Nb-5Zr-2Ta-2Sn (TNZTS alloy)) using a variety of methods, including scanning electron microscopy, transmission electron microscopy, X-ray diffraction, and tensile test. Additionally, the in vitro biocompatibility of the TNZTS alloy was evaluated using SCP-1, SaOs-2, and THP-1 cell lines and primary human osteoblasts. Compared to Ti-6Al-4V, the elastic modulus of TNZTS alloy was significantly lower, while measures of its in vitro biocompatibility are comparable. O2 plasma treatment of the surface of the alloy significantly increased its hydrophilicity and, hence, its in vitro biocompatibility. TNZTS alloy specimens did not induce the release of cytokines by macrophages, indicating that such scaffolds would not trigger inflammatory responses. The present results suggest that the TNZTS alloy may have potential as an alternative to Ti-6Al-4V.

Formation of Titania Submicron-Scale Rod Arrays on Titanium Substrate and In Vitro Biocompatibility

2004 ◽  
Vol 845 ◽  
Author(s):  
Satoshi Hayakawa ◽  
Yongxing Liu ◽  
Kazuya Okamoto ◽  
Kanji Tsuru ◽  
Akiyoshi Osaka
Keyword(s):  
Titanium Surface ◽  
Titanium Substrate ◽  
Sodium Tetraborate ◽  
X Ray Diffraction ◽  
Metallic Titanium ◽  
X Ray ◽  
In Vitro Biocompatibility ◽  
Submicron Scale ◽  
Titanium Substrates

ABSTRACTTitania submicron-scale rod arrays were fabricated on metallic titanium (α-Ti) surfaces by coating a layer of sodium tetraborate on titanium substrates and subsequent thermal treatment. Thin-film X-ray diffraction analysis indicated that the sodium tetraborate gave rutile (TiO2: PDF# 21-1276) submicron-scale rod arrays. The rods in the arrays are parallel to each other in the grain of metallic titanium surface. The titania submicron-scale rod arrays deposited apatite within 7 days after being soaked in a simulated body fluid, indicating that the rod arrays exhibit in vitro bioactivity.

A bioactive collagen-β tricalcium phosphate scaffold for tissue engineering

2006 ◽  
Vol 1 (1) ◽  
pp. 61-72 ◽  
Author(s):  
Elena Oprita ◽  
Lucia Moldovan ◽  
Oana Craciunescu ◽  
Wanda Buzgariu ◽  
Christu Tardei ◽  
...  
Keyword(s):  
Tricalcium Phosphate ◽  
Type I Collagen ◽  
Dermal Fibroblast ◽  
Human Dermal Fibroblast ◽  
Type I ◽  
X Ray Diffraction ◽  
Bioactive Materials ◽  
In Vitro Biocompatibility ◽  
And Migration

AbstractCollagen-phosphate composites (COL/β-TCP) are novel materials that have the potential to be used as bone analogues. The aim of our study was to develop a porous bioactive material composed of type I collagen, the main bone protein and tricalcium phosphate, the mineral phase of natural bone, and investigate their in vitro biocompatibility in a human dermal fibroblast culture system. In order to obtain the bioactive materials, type I collagen was isolated from bovine tendon and characterized by physicochemical methods. β-TCP was obtained from calcium carbonate by thermal decomposition at 900 °C temperature. The powder was examined with X-ray diffraction. Two variants of COL/β-TCP scaffolds (P1 and P2) were prepared and examined by scanning electron microscopy. Our results revealed a microporous structure with small white aggregates of β-TCP, non-homogenous scattered in the collagen framework without any preferential orientation. The biocompatibility of the obtained scaffolds was tested by biochemical and histological methods on human fibroblast cultures. Both materials acted as good subtrates for human dermal fibroblast proliferation and migration.

scholarly journals In vitro characterisation of low-cost synthetic meshes intended for hernia repair in the UK

Hernia ◽  
2021 ◽  
Author(s):  
A. Grillo ◽  
Z. Hyder ◽  
V. Mudera ◽  
A. Kureshi
Keyword(s):  
Low Cost ◽  
In Vivo Studies ◽  
Uniaxial Tensile ◽  
Uniaxial Tensile Test ◽  
Cellular Viability ◽  
In Vitro Biocompatibility ◽  
Two Samples ◽  
Significant Difference ◽  
The Uk

Abstract Purpose Low-cost meshes (LCM) were repurposed for the repair of hernias in the developing world. In vivo studies have shown LCM to have comparable results to commercial meshes (CM) at a fraction of the cost. However, little has been done to characterise the mechanical and biocompatible properties of LCM, preventing its clinical use in the UK. The objectives of the research are to assess mechanical and ultrastructural properties of two UK-sourced low-cost meshes (LCM) and the characterisation of the LCMs in vitro biocompatibility. Methods Mechanical properties of the two LCM were measured through uniaxial tensile test and ultrastructure was evaluated with Scanning Electron Microscopy. LIVE/DEAD® Viability/Cytotoxicity Assay kit and alamarBlue were used to assess cellular viability and proliferation, respectively. Images were acquired with a fluorescence microscope and analysed using ImageJ (NIH, USA). Results LCM1 and LCM2 were both multifilament meshes, with the first having smaller pores than the latter. LCM1 exhibited significantly higher tensile strength (p < 0.05) than LCM2 but significantly lower extensibility (p < 0.0001), while Young’s Modulus of the two samples was not significantly different. No significant difference was found in the cellular viability and morphology cultured in LCM1 and LCM2 conditioned media. Metabolic assay and fluorescence imaging showed cellular attachment and proliferation on both LCMs over 14 days. Conclusion The characterisation of the two UK-sourced LCMs showed in vitro biocompatibility and mechanical and ultrastructural properties comparable to the equivalent CM. This in vitro data represents a step forward for the feasibility of adopting LCM for surgical repair of hernias in the UK.

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