scholarly journals The mechanism of the effects of Monascus jmbA rice on increased platelet count in Wistar rats infected with Dengue virus serotype 3

Author(s):  
Erwin Astha Triyono

Background: Several traditional medicines have been developed among communities as part of the efforts to improve thrombocytopenia in patients with Dengue virus infection (DVI). Among those efforts is the administration of Monascus jmbA rice (MJR), which is rice fermented with Monascus purpureus. The administration of MJR is believed to increase the platelet count in patients with DVI. Aim: The objective of this study is to elucidate the mechanism of the effects of MJR on increased levels of platelets in DVI serotype 3 through changes in IL-3, IL-6, IL-11 and TNF-α. Methods: It was a true experimental laboratory study using the randomized post-test only control group design. The study compared between groups of wistar rats were being treated only with group IVD wistar rats who experienced treatment followed by administration of MJR as well as groups of wistar rats without any treatment as a control group. Results: The increase in platelet count in the group treated with DVI + MJR was higher than that treated with only DVI and the difference was a significant (P<0.05). Increased levels of TNF-α in the group treated with DVI + MJR were lower than that treated with only DVI and the difference was significant (P<0.05). The significant levels of the causal relationship of TNF-α with IL-6, TNF-α with IL-11 and IL-6 with platelets were 0.044 (P<0.05), 0.029 (P<0.05) and 0.041 (P<0.05), respectively. Conclusion: MJR is capable of increasing platelet count through the role of TNF-α and IL-6 in Wistar rats infected with DVI serotype 3.

2018 ◽  
Vol 4 (2) ◽  
pp. 192
Author(s):  
Dwi Gita Oktaviani ◽  
Ahmad Harjono ◽  
I Wayan Gunada

Lesson material presented in the form of data or facts and specific concepts on expository learning model helps learners to connect new material with material that precedes it, so organizers help is provided to instill meaningful learning. This study aims to determine the difference of influence of the implementation of learning model of assisted advance organizer and post organizer expository. The population in this study is all students of class X MIA SMA Negeri 3 Mataram academic year 2017/2018. Sampling is done by purposive sampling technique, and quasi experimental research uses non-equivalent control group design with pre-test-and post-test. The results obtained from the classroom given the treatment of expository learning model assisted by advance organizer and post organizer have the same tendency improvement. In both classes there is a relatively similar increase in the mastery of the previous concept, but the difference between the two is not much different. Therefore, there is no difference of influence between expository model of assisted advance organizer and post organizer to the mastery of work and energy concepts of learners.


2017 ◽  
Vol 1 (1) ◽  
pp. 55
Author(s):  
Rahma Musyawarah ◽  
La Ode Amaluddin ◽  
La Ode Nursalam

This study aims: 1) To know the significance of the difference between the average value of pre-test of experimental class students and the average value of pre-test of control class students, 2) Knowing the average value of post-test of experiment class students is significantly higher compared with the mean value of post-test control class, 3) Knowing the average value of students' gain of experimental class is significantly higher than the control group's average gain. This type of research is experimental research with pretest-posttest control group design. Sample determination technique is random sampling, obtained class X2 as experimental class and class X4 as control class. The data collection technique is done by giving the test of Geography learning result in the form of multiple choice test. Data analysis techniques use descriptive and inferential statistics. The results showed that the average value of post-test students class X2 = 79.67 with n-gain = 0.72 while class X4 = 75.05 with n-gain = 0.63. Based on t test analysis, t-Calculated = 2.07> tTable = 2.00 at a significant level of 95% (α = 0.05), this indicates that the average post-test grade of the experimental class is significantly higher than the the average value of post-test of control class students on the subject matter of the history of earth formation.


DENTA ◽  
2019 ◽  
Vol 12 (2) ◽  
pp. 8
Author(s):  
Isidora Karsini ◽  
Farhana Nur Fadhila ◽  
Nafiah Nafiah

<p><strong><em>Background:</em></strong><em> </em><em>Channa striata contains important compounds in the process of tissue synthesis and important role in wound healing, such as albumin, zinc (Zn), copper (Cu), iron (Fe) and unsaturated fat acids. <strong>Purpose :</strong> To determine the effect of Channa striata extract to the amount of neutrophil in healing process of traumatic ulcer. <strong>Material and Method :</strong> The design of this study used randomized post test only control group design. 18 wistar rats were divided into 6 groups, i.e: K-1 (no treatment for 1 day), K-3 (no treatment for 3 days), K+1 (giving hyaluronic acid 0.2% for 1 day), K+3 (giving hyaluronic acis 0.2% for 3 days), P1 (giving Channa striata extract 100% for 1 day), P3 (giving Channa striata extract 100% for 3 days). <strong>Results :</strong> There was a decrease in the number of neutrophil in the treatment group. The mean and standart deviation of the number of neutrophil in the K-1 group (75,96±3,65), K-3 (50,76±2,04), K+1(72,96±2,97),K+3(45,06±2,83),P1(54,20±1,31),P3(32,50±,85). <strong>Conclusion :</strong> The using of Channa striata extract 100% for 3 days are the most effective in decreasing the amount of neutrophil in healing process of traumatic ulcer of Rattus Novergicus Strain Wistar.</em></p>


DENTA ◽  
2019 ◽  
Vol 12 (2) ◽  
pp. 36
Author(s):  
Agni Febrina Pargaputri ◽  
Dwi Andriani

<p><strong><em> </em></strong></p><p><strong><em>Background: </em></strong><em>Oral candidiasis generally caused by Candida albicans with prevalence of 30-50%. C. Albicans proliferation in the oral cavity was canducted by decreasing immune system of the host. Lymphocytes activation in immune system happened when there is a contact between host cell with cell wall of C. albicans as a result of C. albicans antigen. One of therapy to suppress the Candida infection sistemically is by giving hyperbaric oxygen therapy. <strong>Purpose: </strong>To determine the effects of giving hyperbaric oxygen therapy to the amount of lymphocytes in oral candidiasis immunosuppressed models. <strong>Methods:</strong> This research was post test only control group design. We used 12 wistar rats which were divided into 3 groups(n=4/3): K-(normal/ healthy wistar rats), K+(oral candidiasis immunosuppressed wistar rats), P1(oral candidiasis immunosuppressed wistar rats which were given hyperbaric oxygen therapy 5 days). K+ and P1 groups were immunosuppressed by giving dexamethasone 0,5mg/day/rat orally for 14 days, added with tetrasiklin 1 mg/day/rat. On the 3rd day immunosuppression, the rats were inducted with C.albicans smeared in the dorsum linguae once every two days for 12 days. <strong>Results:</strong> The data were analyzed with one way Anova test and showed significant difference among groups (p&lt;0,05). To show the difference between each groups we used LSD test and showed significant difference (p&lt;0,05) between K+ compared with P1, and K+ compared with K-. <strong>Conclusion:</strong> Hyperbaric oxygen therapy gives effect to the amount of lymphocytes in oral candidiasis immunosuppressed models.</em></p><p><em> </em><strong><em>Keywords:</em></strong><em> Oral candidiasis, immunosuppressed, hyperbaric oxygen therapy, lymphocytes</em></p><p> <strong><em>Correspondence</em></strong><strong><em>: </em></strong><em>Agni Febrina Pargaputri</em><em>, Departmen</em><em>t of</em><em> Oral </em><em>Biology </em><em>F</em><em>aculty of</em><em> </em><em>Dentistry</em><em>, Jl. Arif Rahman Hakim 150, Surabaya, Indonesia. </em><em>Phone:</em><em> 031-5945864, fax: 031-5912191, Email: </em><em><span style="text-decoration: underline;">agni.febrina</span></em><em><span style="text-decoration: underline;">@hangtuah.ac.id</span></em></p><p> </p>


2019 ◽  
Vol 7 (2) ◽  
Author(s):  
PUTU SINTA CANTIKA . ◽  
Drs. Ignatius I Wayan Suwatra,M.Pd . ◽  
Mutiara Magta, S.Pd., M.Pd. .

Penelitian ini bertujuan untuk mengetahui perbedaan perkembangan motorik halus antara kelompok anak yang dibelajarkan menggunakan kegiatan kolase dan kelompok anak yang di belajarkan menggunakan kegiatan mewarnai pada B di TK Gugus VIII Kecamatan Buleleng Tahun Pelajaran 2018/2019. Penelitian ini termasuk jenis penelitan kuantitatif. Penelitian ini tergolog dalam penelitian quasi eksperimen dengan rancanga Non-Equivalen Post Test Only Control Group Desain. Populasi penelitian ini adalah seluruh kelompok B yang ada di Gugus VIII Kecamatan Buleleng Tahun Pelajaran 2018/2019, yang berjumlah 96 orang anak. Sampel penelitian ini yaitu anak kelompok B TK Kumara Kerti yang berjumlah 15 orang sebagai kelas eksperimen dan kelompok B TK Shinta Kumara yang berjumlah 15 orang anak sebagai kelas kontrol, pengambilan sampel menggunakan teknik cluster sampling. Data yang diperoleh menggunakan tehnik analisis statistik deskriptif dan analisis statistik inferensial (uji Uji-t). Berdasarkan hasil analisis data, di peroleh thitung =10,10 dan ttabel (pada taraf taraf signifikansi 5%) =2,048. Hal ini berarti bahwa thitung > ttabel sehingga dapat diinterpretasikan bahwa perkembangan motorik halus kelompok anak yang belajarkan menggunakan kegiatan kolase lebih tinggi dibandingkan dengan kelompok anak yang dibelajarkan dengan kegiatan mewarnai. Dengan demikian, kegiatan kolase berpengaruh positif terhadap perkembangan motorik halus Kelompok B di TK Gugus VIII Kecamatan Buleleng Tahun Pelajaran 2018/2019.Kata Kunci : Kata-kata kunci: Anak usia dini, kegiatan kolase, perkembangan motorik halus The study aims to determine the difference in fine motor development between groups of children that are taught using collage activities and groups of children that are used to use coloring activities on Kelompok B TK GUGUS VIII Kecamatan Buleleng Tahun Pelajaran 2018/2019. This study included quantitative research types. The research has been goed in experimental quasi research with the Non-equivalent Post Test Only Control Group design. The population of this research is Kelompok B Gugus VIII Kecamatan Buleleng Tahun Pelajaran 2018/2019 a total of 96 children. This research sample is the child Kelompok B TK Kumara Kerti which amounted to 15 people as an experimental class while Kelompok B TK Shinta Kumara Which amounted to 15 children as the control class, sampling using cluster sampling technique. Data obtained using descriptive statistical analysis techniques and inferential statistical analysis (test-t test). Based on the results of data analysis, in obtaining Thitung = 10.10 and Ttabel (at the level of significance 5%) = 2.048. This means that Thitung > Ttabel so that it can be interpreted that the fine motor development of a child group that belteaches using a higher collage activity compared to a group of children who are used to the coloring activities. Thus, the collage activities positively impact the development of fine motor Kelompok B TK Gugus VIII Kecamatan Buleleng Tahun Pelajaran 2018/2019.keyword : Keywords: activity, collage, progression, motor, smooth


2021 ◽  
Vol 8 (2) ◽  
pp. 156
Author(s):  
Endah Ariyati Eko Ningtyas ◽  
Oedijani Santoso ◽  
Udadi Sadhana ◽  
Siti Sunarintyas

ABSTRACTBackground: Inflammatory and/or non-inflammatory processes play a role in stimulating pulp repair and the formation of hard tissue, namely reparative dentin. Macrophages play a role in the pathogenesis and chronic inflammatory disorders. The combination casein lactoferrin of bovine colostrum as an immunomodulator has therapeutic potential. This study aims to determine the therapeutic effect and duration of application of the combination of casein lactoferrin of bovine colostrum, on the expression of macrophages as pulp capping.Method: This study was a true experimental laboratories post test only control group design, consisting of three groups of 60 male wistar rats with 4 observation times, namely day to day 7, 14, 21 and 28 each of 5 mice. The maxillary 1st molars were prepared until the roof of the pulp was exposed. Three groups, namely the combination of casein and lactoferrin bovine colostrum (CKL) and calcium hydroxide (K1) and the untreated group (K0). Each group was filled with glassionomer as a permanent restoration. The tissue was made histological preparations with hematoxylin-eosin staining and the number of macrophages were counted, then analyzed by two way ANOVA and post hoc LSD tests.Result: The results showed that the therapeutic effect and duration of application of the combination of casein and lactoferrin bovine colostrum on the expression of macrophages as pulp cappingConclusion: The combination of casein and lactoferrin of bovine colostrum as capping material can increase the number of macrophages in the healing process of dental pulp.


2016 ◽  
Vol 5 (1) ◽  
Author(s):  
Nuriah Arma ◽  
Yanwirasti Ariadi ◽  
Lisma Evareny

AbstrakBayi baru lahir memiliki 80 ml darah dari plasenta pada 1 menit setelah kelahiran dan 100 ml pada 3 menit setelah lahir, volume ini akan memasok 40-50 mg/kg ekstra besi untuk memiliki 75 mg/kg besi tubuh bayi yang cukup bulan yang dapat mencegah kekurangan zat besi pada tahun pertama kehidupan. Oleh karena itu pemotongan tali pusat yang terlalu cepat setelah persalinan akan mengurangi kandungan besi sekitar 15-30%, sedangkan bila ditunda 3 menit dapat menambah volume sel darah merah sekitar 58%. Tujuan penelitian ini adalah untuk membuktikan adanya perbedaan kadar Hb (hemoglobin) dan Ht (hematokrit) akibat perbedaan waktu penjepitan tali pusat. Penelitian ini dilakukan di tempat Bidan Praktek Mandiri Kota Padang. Jenis penelitian eksperimental dengan post-test only controll group design. Pengambilan sampel secara consecutive sampling sampai tercapai jumlah 36 bayi yang terbagi atas 18 bayi baru lahir pada tiap kelompok. Data dianalisis dengan uji t. Hasil penelitian didapatkan rerata kadar Hb dan Ht pada kelompok penjepitan 3 menit setelah lahir lebih tinggi daripada penjepitan 1 menit. Terdapat perbedaan kadar Hb yang bermakna antara kelompok penjepitan 3 menit setelah lahir daripada 1 menit dengan nilai P=0,004.  Ada terdapat perbedaan Ht yang bermakna antara kelompok penjepitan 3 menit dan 1 menit dengan nilai P=0,001. Kesimpulan penelitian ini ialah kadar Hb dan Ht lebih baik pada penjepitan tali pusat ditunda 3 menit setelah lahir dibandingkan dengan penjepitan 1 menit setelah lahir.Kata kunci: hemoglobin, hematokrit, penjepitan tali pusat AbstractThe newborns contain 80 ml of blood from the placenta in 1 minute after birth and 100 ml in 3 minute after birth. This volume supply 40 to 50 mg/kg of extra iron to have 75 mg/kg of body iron baby full-term that can prevent iron deficiency in the first year of life. Therefore, cutting the umbilical cord too soon after birth will reduce the iron of content about 15 to 30%, whereas when delayed 3 minute its can increase the volume red blood cells about 58%. The objective of this study was to prove the difference of hemoglobin and hematocrit level between 3 minutes and 1 minute cord clamping.  Experimental research was conducted with post-test only control group design of 36 newborns. Data were analyzed by t-test. The result of this study showed that the mean of hemoglobin and hematocrit level in 3 minutes of clamp are higher  than 1 minute of clamp.There was a significant difference of hemoglobin level between 3 minutes cord clamping group and 1 minute cord clamping group with p value is 0.004. There was also a significant difference of hematocrit level between 3 minutes cord clamping group and 1 minute cord clamping group with p value is 0.001. The conclusion is the hemoglobin and hematocrit level are better in cord clamping delayed in 3 minutes after birth than clamping in 1 minute after birth. Keywords: hemoglobin, hematocrit, cord clamping


e-CliniC ◽  
2013 ◽  
Vol 3 (2) ◽  
Author(s):  
Eny A. Watumlawar ◽  
Sarah M. Warouw ◽  
Stefanus Gunawan

Abstract: Weight is one measure that gives description of the tissue mass including body fluids. Weight change is nfluenced by nutriment consumption. Sago contains high carbohydrates that can increase body weight. This study aimed to obtain the effect of sago compared to rice to body weight of wistar rats. This was a random laboratory experimental pre-post test with control group design. Subjects were male wistar rats, aged 5-6 months. The rats were divided in two groups: rice group as control and sago group. Sago was cooked in papeda form as much as 75 g of dried sago and 300 mL water. The rats were fed for 2 weeks. Data were analyzed by using the Wilcoxon test. The results showed that the sago group showed an increase of body weight significantly (p=0,001) meanwhile the rice group lose body weight significantly (p=0,001). Conclusion: Sago can increase body weight of wistar rats significantly.Keywords: body weight, sago, riceAbstrak: Berat badan merupakan salah satu ukuran yang memberikan gambaran massa jaringan termasuk cairan tubuh. Salah satu yang memengaruhi berat badan yaitu dengan mengonsumsi makanan bergizi. Sagu memiliki kandungan karbohidrat (pati) yang besar dan dapat meningkatkan berat badan. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian sagu terhadap berat badan dibanding nasi pada tikus wistar. Desain penelitian ini ialah eksperimental laboratorium acak pre-post test with control group design. Subjek penelitian ialah tikus wistar jantan berusia 5-6 bulan dibagi atas 2 kelompok: kelompok nasi (kontrol) dan kelompok sagu. Sagu dimasak dalam bentuk papeda sebanyak 75 g sagu kering dan 300 mL air. Tikus diperlihara selama 2 minggu. Berat badan diukur dengan menggunakan timbangan. Data dianalisis dengan uji Wilcoxon. Hasil penelitian menunjukkan bahwa pemberian sagu sebanyak 2 cc setiap hari selama seminggu mampu meningkatkan berat badan secara bermakna (p = 0,001) sedangkan pemberian nasi menurunkan berat badan (p=0,001). Simpulan: Pemberian sagu dapat meningkatkan berat badan tikus wistar secara bermakna.Kata kunci: berat badan, sagu, nasi


2021 ◽  
Vol 21 (no 1) ◽  
Author(s):  
Gede Widhiantara ◽  
Anak Agung Ayu Putri Permatasari ◽  
Putu Angga Wiradana

This study aims to determine the morphology of Leydig cells in hyperlipidemic wistar rats after having administered with sembung (Blumea balsamifera) extract orally. This study utilised a randomized post-test only control group design. The sample in this study were 16 adult male wistar rats (Rattus novergicus) aged 3-4 months with a body weight of 150-200 grams, which were equally and randomly divided into two groups, namely control group (hyperlipidaemia and sterile aquadest) and treatment group (hyperlipidaemia and sembung leaf extract). Hyperlipidaemia was induced with lard administration for 50 days. Data were analysed statistically using the Independent t-test. The results showed that the number of Leydig cells in the treatment group was higher than the control group with 68.13 ± 1.89 and 55.63 ± 1.92 cells respectively (P <0.05). In addition, the mean Leydig cell core diameter of the control group, 5.00 ± 0.34 µm, was smaller compared to the treatment group which was 5.80 ± 0.20 µm (P <0.05). It can be inferred that sembung leaf extract provides a protective effect against damage to Leydig cells due to hyperlipidaemia.


2019 ◽  
Vol 47 (2) ◽  
pp. 35-37
Author(s):  
Kusbaryanto ◽  
Ekorini Listiyowati

Nosocomial infection is a major problem in hospitals around the world. To minimize the risk of infection in hospitals and other healthcare facilities, cough etiquette has to be applied. Thus, good situation awareness and the understanding why cough etiquette is important are necessary. The purpose of this study was to find out the effective education on cough etiquette on hospital security personnel’s and janitors’ situation awareness on cough etiquette. This research study followed quasy experimental design with pretest and post test control group design. The sampling technique was purposive sampling involving 24 respondents for experimental group and 27 respondents for the control group. The analysis of the data employed Wilcoxon test and it employed Mann Whitney test to find out the difference of independent variable medians. Questionnaires were used to gather data collection. The result of the analysis presented a mean of 97.7 on the post-test of control group and a mean of 116.3 on the post-test of treatment group. Wilcoxon and Mann Whitney tests resulted   p = 0.001 ( < 0.05 ), which meant there were significant differences. Education on cough etiquette is effective to raise hospital security personnel and the janitors’ awareness on cough etiquette situation. Bangladesh Med J. 2018 May; 47 (2): 35-37


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