scholarly journals Evaluation the capacity of microbiological laboratories through proficiency testing programs in 2017

2018 ◽  
Vol 1 (2) ◽  
pp. 14-20
Author(s):  
Hang Nguyen Thi ◽  
Huong Doan Thi ◽  
◽  
Keyword(s):  
Quality Assurance ◽  
Proficiency Testing ◽  
Functional Food ◽  
Statistical Processing ◽  
Pass Rate ◽  
Plate Count ◽  
Test Results ◽  
Food Control ◽  
E Coli ◽  
Total Plate Count

In the context of international integration into the WTO, quality assurance in laboratories is an important requirement for providing reliable and highly accurate test results. One of the quality assurance tools currently used by various laboratories is the participation in proficiency testing (TNTT)/ interlaboratory comparisons. In order to assess the capacity of microbiological laboratories throughout the country and respond to the requirements of the customers, the National Institute for Food Control (NIFC) organized 6 biological proficiency testing schemes in food and functional foods in 2017. Based on the statistical processing and z-score calculation, NIFC has made a list of highly satisfactory microbiological laboratories such as such the pass rate of Total Plate Count, E. coli, Coliforms in functional food with 92.9%, 100%, 92.3% ... The highest failure rate of Yeast and Mould in functional food (35.7%) and Coliforms in water (47.8%)... The results showed that the capacity of microbiology laboratories has been increased in both quality and quantity (with the largest number of participants of 89 laboratories).  

scholarly journals Pengaruh Lama Pemakaian Sediaan Kosmetik Bedak Padat Terhadap Cemaran Mikroba

2020 ◽  
Vol 3 (1) ◽  
Author(s):  
Munira Munira ◽  
Cut Fardilla ◽  
Noni Zakiah ◽  
Rasidah Rasidah ◽  
Muhammad Nasir
Keyword(s):  
Bacterial Contamination ◽  
Plate Count ◽  
Test Results ◽  
Food Control ◽  
Average Value ◽  
Total Plate Count ◽  
Compact Powder ◽  
Long Time ◽  
The Republic ◽  
Number Of Bacteria

Bedak adalah salah satu sediaan kosmetik yang dipakai dalam jangka waktu yang lama. Bedak yang digunakan akan terjadi kontaminasi bahkan semakin meningkat seiring dengan lamanya pemakaian. Faktor-faktor yang mempengaruhi antara lain penyimpanan, lingkungan, dan spons bedak. Penggunaan spons bedak merupakan salah satu sumber cemaran bakteri. Di mana penggunaan spons yang sama secara berulang-ulang dan bersentuhan langsung pada kulit dapat meningkatkan jumlah mikroba. Tujuan penelitian ini adalah untuk mengetahui pengaruh lamanya penyimpanan, pemakaian suatu sediaan kosmetik bedak padat terhadap tingkat cemaran mikroba. Sampel yang diuji adalah bedak padat yang belum dipakai dan yang telah dipakai selama 2 minggu. Hasil penelitian berupa jumlah cemaran bakteri (ALT) dibandingkan dengan syarat menurut Peraturan Kepala Pengawasan Obat dan Makanan RI No.HK.03.1.23.07.11.6662 Tahun 2011 yaitu tidak boleh melebihi 103 koloni/g. Hasil uji cemaran bakteripada sediaan bedak padat sebelum pemakaian mempunyai nilai rata-rata ALT 2,6 x 101koloni/g dan sesudah pemakaian mempunyai nilai rata-rata ALT 9,2 x 101 koloni/g. Nilai ALT yang diperoleh dari masing-masing sediaan kosmetik bedak padat telah memenuhi syarat.Hasil uji t dependen menunjukkan adanya perbedaan jumlah bakteri pada bedak padat sebelum dipakai dengan setelah dipakai selama 2 minggu (t = 0.000). Kata Kunci: Kosmetik, bedak padat, Angka Lempeng Total ABSTRACTPowder is one of the cosmetic preparations used for a long time. Powder that is used will occur contamination even increasing along with the length of usage. This can be caused by several factors including storage, environment, powder sponges and others. The use of powder sponges is one source of bacterial contamination. Where the use of the same sponge repeatedly and in direct contact with the skin can increase the number of microbes. The objective of study was to determine the effect of the duration of use of a compact powder cosmetic preparation on the level of microbial contamination. The samples tested were solid powder that has not been used and that has been used for 2 weeks. The results of the study were the Total Plate Count (TPC) compared to the Regulation of the Head of the Republic of Indonesia Drug and Food Control Number HK.03.1.23.07.11.6662 of 2011 concerning the Requirements for Microbial Pollution and Heavy Metals in Cosmetics which should not exceed 103 colonies / g. The test results of bacterial contamination on solid powder preparations before use have an average value of ALT 2,6 x 101 colony / g and after use have an average ALT value of 9.2 x 101 colony / g. The ALT value obtained from each compact powder cosmetic preparation has met the requirements. The results of the dependent t test showed a difference in the number of bacteria on solid powder before use with after being used for 2 weeks (t = 0.000). Keywords: Cosmetics, Compact Powder, Total Plate Count

scholarly journals ANALISA MIKROBIOLOGI DAN ORGANOLEPTIK PRODUK TENGGIRI BEKU(Scomberomorus commersonii)

2018 ◽  
Vol 6 (2) ◽  
pp. 54
Author(s):  
Mulyana Mulyana ◽  
Dwi Indah Widya Yanti
Keyword(s):  
Plate Count ◽  
Test Results ◽  
Microbiological Test ◽  
E Coli ◽  
Total Plate Count ◽  
Physical Chemical ◽  
The People ◽  
Standard Value ◽  
Test Result

Mackerel fish is sea fishs species which are pelagic marine fish groupwhich has a distinctive taste so favored by the people. frozen mackerel is a fishs that is easily damaged. Thus the fishs need to be handling it well. Fishskeep the temperature will affect the quality of the fishs. during storage in the storage and distribution processing. can affect product quality. To decrease the quality of frozen mackerel fishs products need to note the changes that occur either through physical, chemical and biological. It explains research to analyze the quality of fishs products frozen mackerel observe  aspects of Microbiology testing and Appearance. Based on the results of microbiological and organoleptic frozen mackerel fish, it can be conclude that test for microbiological test total plate count (TPC) is below the standard value in accordance with ISO 4110, 2014. for Uju E, coli is negative, Salmonella Test is Begatif, Vibrio Colera test is also negative and Appearance Test Values above 7 quality fish so the test results of Microbiology and poduct organoleptic frozen mackerel safe for consumption as the above test result.

scholarly journals Implementasi Higiene Sanitasi pada RPH Kategori I sebagai Syarat Produksi Daging Sehat

2021 ◽  
Vol 9 (3) ◽  
pp. 138-143
Author(s):  
M. Fitri ◽  
H. Nuraini ◽  
R. Priyanto ◽  
Y. C. Endrawati
Keyword(s):  
Water Quality ◽  
Quality Assurance ◽  
Assessment Method ◽  
Personal Hygiene ◽  
Plate Count ◽  
E Coli ◽  
A Value ◽  
Total Plate Count ◽  
Sanitation And Hygiene ◽  
Quality Testing

Abbatoirs have to produce meat that complied requisite of safety, healthy, wholeness and halal. Animalorigin product have to requisite basic safety product which implementated of hygine sanitation asproduction processed. The research aim was to evaluate implementation of hygine sanitation systemon 1st categrory abbatoir at Bogor Regency to complied safety and quality assurance of product.Observations on the implementation of hygienic sanitation were carried out using an evaluation matrixof the suitability of physical requirements with the criterion parameter assessment method based onthe Regulation of the Minister of Agriculture Number 13/2010. The best suitability value (NK) of 3 wasobtained on the environmental hygiene indicator and the absence of the use of chemicals, while theother sanitation hygiene indicators were worth 2 (less appropriate). The results of testing on meat forthe content of Salmonella sp. is negative and the Total Plate Count test is between 7.1 x 103 - 5.2 x 104cfu/g below the SNI threshold (1.0 x 106 cfu/g). The test for E. coli in meat was above the threshold ofsuspected contamination from the water used. Validation of hygiene sanitation practices was carried outby means of Colliform swabs on knives, palms hand and clothing of officers. Colliform swab test of theofficer’s palm hand, which is 5.4 x 102 cfu/g, shows a value above the allowable threshold. The results ofthe evaluation of the application of sanitation and hygiene in the abattoir are quite good but still needimprovement. Priority improvements are the equipment of cleaning facilities, water quality testing andimprovement of personal hygiene understanding.

scholarly journals AKTIVITAS ANTIMIKROBA YOGURT BERBASIS AIR KELAPA MENGHAMBAT BAKTERI PATOGEN SECARA IN VITRO

1970 ◽  
Vol 17 (2) ◽  
Author(s):  
Lindawati S. A. ◽  
Haniyah Y. S. ◽  
Miwada I N. S. ◽  
Inggriati N. W. T. ◽  
Hartawan M. ◽  
...  
Keyword(s):  
Salmonella Typhi ◽  
Lactobacillus Bulgaricus ◽  
Plate Count ◽  
Klebsiella Pneumonia ◽  
E Coli ◽  
Total Plate Count

Yogurt merupakan minuman susu yang difermentasi dengan menggunakan bakteri asam laktat Lactobacillus bulgaricus dan Streptococcus thermophyllus sebagai starter. Tujuan penelitian untuk mengetahui kemampuan tertinggi dari yogurt berbasis air kelapa dalam menghambat pertumbuhan bakteri pathogen (Salmonella, Staphylococcus, Klebsiela pneumonia dan E.coli) secara in vitro. Penelitian ini menggunakan rancangan acak lengkap (RAL) dengan empat perlakuan dan empat ulangan. Keempat perlakuan tersebut: Ko: susu skim 12%+air; K1: susu skim 12%+air kelapa gading; K2: susu skim 12%+kelapa gading bulan; K3: susu skim 12%+air kelapa hijau. Peubah yang diamati adalah aktivitas antimikroba yogurt terhadap bakteri pathogen (Salmonellatyphi, Staphylococcus, Klebsiella pneumonia dan E.coli), total plate count (TPC), total bakteri asam laktat dan E,coli. Hasil penelitian menunjukkan bahwa aktivitas antimikroba tertinggi diperoleh pada yogurt berbasis air kelapa bulan terhadap bakeri Salmonella typhi dan Staphylococcus masing-masing sebesar 0,21 dan 0,26 cm dengan total bakteri asam laktat1,1×107 cfu/mL sedangkan untuk Klebsiella pneumonia dan E.coli diperoleh pada yogurt berbasis air kelapa gading sebesar 0,38 dan 0,64 mm dengan total bakteri asam laktat 3,1×106 cfu/mL.Dari hasil penelitian dapat disimpulkan bahwa yogurt berbasis air kelapa gading, bulan, hijau dan tanpa air kelapa memiliki aktivitas antimikroba berspektrum luas (menghambat bakteri gram positif dan negatif).

scholarly journals KEBERADAAN MIKROBA PADA BAKSO IKAN ASAP CAIR, YANG DIKEMAS DALAM RETORTABLE POUCH, DIPASTEURISASI DAN DISIMPAN PADA TEMPERATUR RUANG

2016 ◽  
Vol 4 (2) ◽  
pp. 85
Author(s):  
Margani Luyuani Rohana ◽  
Siegfried Berhimpon ◽  
Joyce CV Palenewen
Keyword(s):  
Shelf Life ◽  
Water Content ◽  
Experimental Method ◽  
Room Temperature ◽  
Plate Count ◽  
E Coli ◽  
Existing Problems ◽  
Liquid Smoke ◽  
Total Plate Count ◽  
And Storage

A research has been done to assess the presence of microbes and the shelf life of fish balls dipped in liquid smoke, packed in retortable pouch, pasteurized, and stored at room temperature. The method used in this research is an experimental method that revealed the facts based on existing problems through hypothesis testing. Parameters assessed are Total Plate Count (TPC), total Salmonella, total coliforms and E. coli, total vibrio, water content, and pH. Fish balls were pasteurized in 85ºC and stored in room temperature for 0, 3, 6, and 9 days, and were pasteurized at 100°C and storage in room temperature for 0, 9, 18, and 27 days. The results shown that all pathogen were negative, but based on Indonesian standard (SNI 01-7266-1-2006) especially TPC value, fish balls were packaged in retortable pouch and without pasteurized is no longer acceptable for consumption after 3 days of storage, and fish balls were packaged in retortable pouch and pasteurized at 85ºC no longer acceptable for consumed after 6 days of storage. While the fish balls are packaged in retortable pouch and pasteurized at 100°C is no longer acceptable for consumed after 18 days of storage.Keyword: Retortable Pouch, Pasteurization, Pathogens.  Penelitian ini bertujuan untuk mengetahui keberadaan mikroba dan daya awet bakso ikan yang direndam dalam asap cair dan dikemas dalam Retortable pouch, dipasteurisasi, dan disimpan pada temperatur ruang. Metode yang digunakan dalam penelitian ini adalah metode eksperimen untuk menguji hipotesa. Parameter yang diamati yaitu Angka Lempeng Total (ALT), Salmonella, total koliform dan E.coli, total vibrio, analisa kadar air, dan nilai pH. Bakso ikan asap cair yang dipasteurisasi pada 85ºC, disimpan pada temperatur ruang selama 0, 3, 6, dan 9 hari, dan yang dipasteurisasi pada 100ºC, disimpan pada temperatur ruang selama 0,9,18, dan 27 hari. Hasil penelitian menunjukkan bahwa bakso ikan yang dikemas dalam Retortable pouch, tanpa dipasteurisasi sudah tidak layak lagi untuk dikonsumsi setelah penyimpanan 3 hari, dan bakso ikan yang dikemas dalam Retortable pouch dan dipasteurisasi pada 85ºC sudah tidak layak lagi untuk dikonsumsi setelah penyimpanan 6 hari. Bakso ikan yang dikemas dalam Retortable pouch, dan dipasteurisasi pada 100ºC nanti tidak layak lagi dikonsumsi setelah penyimpanan 18 hari.Kata Kunci: Kemasan Retortable Pouch, Pasteurisasi, Patogen.

scholarly journals KOMPOSISI KIMIA, SENYAWA BIOAKTIF DAN ANKGA LEMPENG TOTAL PADA RUMPUT LAUT Gracillaria edulis

2017 ◽  
Vol 5 (3) ◽  
pp. 90
Author(s):  
Susanti Musa ◽  
Grace Sanger ◽  
Henny Adeleida Dien
Keyword(s):  
Moisture Content ◽  
Proximate Composition ◽  
Bioactive Compound ◽  
Bacterial Count ◽  
Ash Content ◽  
Plate Count ◽  
Test Results ◽  
Gracilaria Edulis ◽  
Average Value ◽  
Total Plate Count

This study was conducted to determine the proximate composition, bioactive compound and total plate count (TPC) in Gracilaria edulis seaweed. This research used descriptive explorative method. The results showed the average value of moisture content (77.7%); protein content (39.4%); fat content (8.1%); carbohydrates by difference (71.7%); ash content (20.7%) and very low bacterial count. Phytochemical test results show this seaweed contains alkaloids, saponins, flavonoids, and triterpenoids compounds. Penelitian ini dilakukan untuk mengetahui komposisi proksimat, senyawa bioaktif dan angka lempeng total (ALT) pada rumput laut Gracilaria edulis. Penelitian ini menggunakan metode penelitian deskriptif eksploratif. Hasil penelitian menunjukkan nilai rata-rata kadar air (77,7%); protein (39,4%); kadar lemak (8,1%); karbohidrat (71,7%); kadar abu (20,7%) dan jumlah bakteri yang sangat rendah. Hasil uji fitokimia menunjukkan rumput laut ini mengandung  senyawa alkaloid, saponin, flavonoid, dan triterpenoid.

scholarly journals KARAKTERISTIK KOMPOSISI KIMIA DAN TOTAL BAKTERI IKAN ROA (Hemirhampus sp.) ASAP YANG DI PASARKAN DI KECAMATAN BUNGKU TENGAH KABUPATEN MOROWALI

2020 ◽  
Vol 3 (1) ◽  
pp. 113
Author(s):  
Sarni Sarni ◽  
Haslianti Haslianti ◽  
Suwarjoyowirayatno Suwarjoyowirayatno
Keyword(s):  
Chemical Composition ◽  
Random Sampling ◽  
Descriptive Analysis ◽  
Simple Random Sampling ◽  
Plate Count ◽  
Survey Method ◽  
Test Results ◽  
Total Plate Count ◽  
Total Bacteria ◽  
Treatment Water

Characteristics of Chemical Composition and Total Bacterial of Roa (Hemirhampus sp.) Smoke Marketed in the Bungku District of Central Morowali DistrictABSTRACT         The aim of this study was to determine the characteristics of chemical composition (water, ash, protein, and fat) and total bacteria (TPC) of smoked roa fish. Data analysis this study used descriptive analysis. The method used study is the survey method (Simple Random Sampling). The results of the chemical composition of the highest treatment water content P2 13.02%, ash P3 5.65%, protein P3 40.82%. and fat P3 13.87%. The best organoleptic test results were found in P1 with 7.30 appearance, 8.20 odor, 8.00 taste, 7.80 texture, and the best mushroom specifications were at P2 9.00. The lowest total bacteria test was found with 0,83×105 Log CFU/gr.Keywords: Ikan roa (Hemirhampus sp.) Asap, Total Plate Count (TPC),Uji Kimia, dan Uji Organoleptik ABSTRAKTujuan penelitian ini adalah untuk mengetahui karakteristik komposisi kimia  (kadar air, kadar abu, kadar protein dan kadar lemak) dan total bakteri (TPC) terhadap ikan roa asap. Analisis data yang digunakan dalam penelitian ini yaitu menggunakan analisis deskriptif. Metode yang digunakan dalam penelitian ini yaitu metode survey (Simple Random Sampling). Hasil penelitian komposisi kimia perlakuan tertinggi kadar air P2 13,02%. Kadar abu tertinggi terdapat pada perlakuan P3 5,65%. Kadar protein perlakuan tertinggi terdapat pada P3 40,82%. dan kadar lemak perlakuan tertinggi terdapat pada  P3 13,87%. Hasil penilaian uji organoleptik terbaik terdapat pada P1 sepesifikasi kenampakan 7,30, bau 8,20, rasa 8,00, tekstur 7,80 dan pada spesifikasi jamur nilai terbaik terdapat pada P2 9,00. Total bakteri terendah terdapat pada P3 dengan nilai 0,83×105 Log CFU/gr.Kata kunci: Ikan roa (Hemirhampus sp.) Asap, Total Plate Count (TPC),Uji Kimia, dan Uji Organoleptik

scholarly journals Tailed Pepper (Piper cubeba) L. berries extract reduced number of microbial population in tofu

Food Research ◽  
2020 ◽  
Vol 4 (3) ◽  
pp. 738-745
Author(s):  
F.M. Alqadeeri ◽  
Faridah Abas ◽  
Khozirah Shaari ◽  
Yaya Rukayadi
Keyword(s):  
Bacillus Cereus ◽  
Foodborne Pathogens ◽  
Microbial Population ◽  
Antimicrobial Activities ◽  
Plate Count ◽  
Natural Preservative ◽  
E Coli ◽  
Total Plate Count ◽  
The Family ◽  
Natural Microflora

The family of Piperaceae contains the species P. cubeba L., which has been used as a spice in countries such as Malaysia, Indonesia, India, Morocco, and Europe. A previous study has shown that crude extracts of tailed pepper (P. cubeba L.) have antimicrobial activities against foodborne pathogens species. The excellent antimicrobial activity of P. cubeba L. berries extract makes it suitable for use as a natural preservative or sanitizer in the food. The aim of this study was to evaluate the effect of the P. cubeba L. berries extract on microbial population in tofu. The tofu samples were cut into small pieces and mixed thoroughly to ensure the homogeneity of natural microflora. Ten grams of the samples were immersed in 20 mL varying concentrations of P. cubeba L. extract; 0.00%, 0.05%, 0.50% and 5.00% for 1, 2 and 4 hrs at room temperature (23±2°C) with the agitation of 50 rpm. At 1, 2 and 4 hrs the numbers of total plate count (TPC), Bacillus cereus, coliform and Escherichia coli were counted. The result shows that a reduction of at least 3 Log10 CFU/g of TPC, Bacillus cereus., coliform and E. coli in tofu samples was observed when the samples were treated with 0.50% extract for four hours. The result suggested that P. cubeba L. berries extract can be used as a natural preservative to reduce the microbial load in raw food.

scholarly journals Quality of Dried Anchovy (Stolephorus sp.) from Buru Island Produced by Using Closed Sun Dryer

2018 ◽  
Vol 21 (3) ◽  
pp. 543
Author(s):  
Imelda Krisanta Enda Savitri ◽  
Bernita Silaban ◽  
R.B.D. Sormin
Keyword(s):  
Traditional Method ◽  
Low Cost ◽  
National Standard ◽  
Plate Count ◽  
Drying Method ◽  
Vibrio Cholera ◽  
E Coli ◽  
Total Plate Count ◽  
Better Than

Anchovies drying method in Saliong, Batuboi village, Kayeli Bay has been done by spreading fish on the ground covered by mat or net due to low cost and easy to apply. However, there are no guarantee in the sanitation and hygienic. The objective of this research was to study the effect of closed sun drying method on the quality of dried anchovies (Stolephorus sp). The method was using closed cabinet sun dryer prototype covered by transparence plastic multi racks. Parameters observed involve: organoleptic, moisture content, acid insoluble, total plate count (TPC), E. coli, Salmonella sp., Vibrio cholera and fungi according to Indonesian national standard reference (SNI). The result showed the dried anchovy have an organoleptic grade refers to appearance, smells and texture were 8.40, 8.33 and 8.27 respectively. Moreover, moisture 17.50%; acid insoluble ash 0.32%; total bacterial (TPC) 6.92x102; E. coli, Salmonella sp., V. cholera and fungi were negative. For comparison, dried anchovy produced by using conventional/traditional drying had an organoleptic grade refers to appearance, smells and texture were 7.67, 7.73 dan 7.87 respectively; while moisture 16.93% ; acid insoluble ash 0.65%; total bacterial (TPC) 1.16 x103; E. coli, Salmonella sp., V. cholera and fungi were negative. According to the SNI 01-2891 BSN 1992, dried anchovy produced by using enclosed sun dryer better than dried anchovy produced by traditional method.

scholarly journals "Base Genep" Instant Spices Quality For Sale In East Denpasar

2020 ◽  
Vol 4 (2) ◽  
pp. 142-150
Author(s):  
Sofia nggoweng ◽  
Ni Made Ayu Suardani singapurwa ◽  
I Nyoman Rudianta
Keyword(s):  
Escherichia Coli ◽  
Moisture Content ◽  
Water Content ◽  
Ph Value ◽  
Descriptive Study ◽  
Experimental Methods ◽  
Plate Count ◽  
Test Results ◽  
Total Plate Count

Instant spices is a mixture of several spices that are young to be found in the market at an affordable price, and are usually used immediately as a cooking spice for certain foods. This study aims to determine the quality of the "Base Genep" instant spices sold in the East Denpasar Market. This research is a descriptive study using survey and experimental methods in the laboratory. The number of samples to be studied was 44 samples from 81 populations. The variables in the study were moisture content, pH, Escherichia coli, Total Plate Count (TPC) and mold. The results showed that from 44 samples of "Basa Genep" instant seasoning water content test results obtained were 21.62% - 23.707%, while the pH value of 44 samples of "Basa Genep" instant spice was obtained between 2.2-2.5 so that it meets the requirements.

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