ANTIOXIDANT ACTIVITY AND CYTOTOXIC OF N-hexane AND METHANOL EXTRACT FROM MANGO LEAVES (Mangifera indica L.)

2020 ◽  
Vol 5 (2) ◽  
pp. 124-134
Author(s):  
Vina Juliana Anggraeni
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Methanol Extract ◽  
Mangifera Indica ◽  
Mango Leaves

Telah dilakukan uji antioksidan dan sitotoksik terhadap ekstrak n-heksana dan metanol daun mangga (Mangifera indica L.). Daun mangga diekstrak menggunakan metode maserasi bertingkat menggunakan 2 pelarut yang berbeda yaitu pelarut n-heksana dan metanol. Hasil maserasi dipekatkan kemudian diuji aktivitas antioksidan dan sitotoksiknya. Uji aktivitas antioksidan menggunakan metode spektrometri dengan pereaksi DPPH dan pembandingnya berupa vitamin C. Sedangkan untuk uji sitotoksik menggunakan metode BLST. Hasil pengujian menunjukkan kedua ekstrak memiliki aktivitas antioksidan dan sitotoksik. Pada ekstrak metanol diperoleh IC50 sebesar 13,54 ppm dan LC50 sebesar 260,07 ppm. Sedangkan pada ekstrak n-heksana diperoleh IC50 sebesar 730,03  ppm dan LC50 sebesar 711,73 ppm. Untuk standar DPPH menggunakan vitamin C dengan IC50 sebesar 5.13 ppm. Aktivitas antioksidan dan sitotoksik ekstrak metanol lebih kuat dibandingkan dengan esktrak n-heksana.

FORMULATION AND CHARACTERIZATION OINMENT OF ARUM MANIS MANGO (Mangifera indica L.) LEAVES METHANOL EXTRACTS AS AN ANTIBACTERIAL AGAINST Propionibacterium acnes

2020 ◽  
Vol 16 ◽  
Author(s):  
Dian Riana Ningsih ◽  
Zusfahair Zusfahair ◽  
Dadan Hermawan ◽  
Wulan Anggraeni ◽  
Hassan Y. Aboul-Enein
Keyword(s):  
Inhibitory Concentration ◽  
Propionibacterium Acnes ◽  
Methanol Extract ◽  
Mangifera Indica ◽  
Inhibition Zone ◽  
Sweat Glands ◽  
Ointment Formulation ◽  
Inhibition Zones ◽  
Mango Leaves ◽  
Dispersive Power

Background: Acne is caused by several factors including the active secretion of sebaceous sweat glands, hyperkeratosis in the hair infundibulum and the effects of bacteria. One of the plants that has the potential as an antibacterial is the extract of arumanis mango leaves. Method: Determine the Minimum Inhibitory Concentration (MIC) of methanol extract of mango leaves which can inhibit Propionibacterium acnes activity. The antibacterial activity tests were performed using agar diffusion. The ointment formulation, the characteristics of ointment preparations and the ointment activity against P. Acnes are discussed. Result: MIC of methanol extract of mango leaves value is 5 ppm with an inhibition zone of 1 mm. The ointment obtained is white, has distinctive smell, semisolid form, possesses a pH of 4.92 - 5.87, dispersive power of 5.05 - 6.30 cm, adhesive power of 1 - 3.67 seconds, homogeneous and protective. Ointment preparations of methanol extract of mango leaves has activity on P. acnes on the 0 and 15th day of storage. The activities of ointment preparation on day 0 with concentrations of 0, 5, 10 and 15 ppm are 0.00 mm; 10.20 mm; 19.97 mm and 23.60 mm respectively, while the inhibition zones produced by the preparation of ointment on day 15 with concentrations of 0, 5, 10 and 15 ppm are 0.00 mm; 5.71 mm; 9.58 mm and 21.88 mm respectively. Conclusion: Methanol extract of mango leaves (Mangifera indica L.) and oinment preparation are able to inhibit the growth of Propionibacterium acnes.

scholarly journals A Comparative Assessment on Antioxidant and Phytochemical of Trichilia monadelpha (Thonn) J.J. De Wilde (Meliaceae) Plant Extracts

2021 ◽  
pp. 24-33
Author(s):  
P. D. Clark ◽  
E. Omo-Udoyo
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Plant Extracts ◽  
Methanol Extract ◽  
Methanolic Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Scavenging Activity ◽  
Hydroxy Radical ◽  
Reducing Ability

This study was designed to evaluate the phytochemical screening and antioxidant activities of Trichilia monadelpha plant extracts. The leaves were extracted using two different solvents namely-(n-hexane and methanol).  The dry or wet yield of n-hexane extract was 1.3%, while methanolic extract exhibited a percentage yield of 3.7 %.  Phytochemical research revealed the presence of secondary plant metabolites such as; alkaloids, flavonoids, cardiac glycosides, terpenoids and saponins. In-vitro antioxidant activity was determined using three assays (DPPH free radical scavenging assay, reducing ability and hydroxy radical scavenging activity) with four concentrations (0.25, 5.0, 1.0 and 2.0 mg/L), vitamin C was also used as a standard antioxidant. The percentage of inhibition was measured, and the results from all assay models showed a concentration-dependent percentage of inhibition by the methanol extract. However, the percentage values for the 2,2, -diphenyl-1-picrylhydrazyl (DPPH), reducing ability and hydroxy radical scavenging activity assays of T. monadelpha leaf was lower than that of standard vitamin C. The Pearson’s correlation coefficient was evaluated and the results showed that of the two extracts, the methanolic extract had the most antioxidant activity with the methanol extract exhibiting a better significant correlation that had a similar trend to that of the antioxidant compound (vitamin C). The results of this study have shown that the plant Trichilia monodelpha contains bioactive compounds which may have contributed to its antioxidant properties.

scholarly journals FORMULASI DAN AKTIVITAS ANTIOKSIDAN MASKER WAJAH GEL PEEL OFF EKSTRAK METANOL BUAH PEPAYA (Carica papaya L.)

2018 ◽  
Vol 1 (2) ◽  
Author(s):  
Liza Pratiwi ◽  
Sri Wahdaningsih
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Carica Papaya ◽  
Methanol Extract ◽  
Gelling Agent ◽  
Adhesion Test ◽  
Physical Test ◽  
Facial Mask ◽  
Ic50 Value ◽  
Spreading Test

FORMULASI DAN AKTIVITAS ANTIOKSIDAN MASKER WAJAH GEL PEEL OFF EKSTRAK METANOL BUAH PEPAYA (Carica papaya L.)Liza Pratiwi1), Sri Wahdaningsih1)1) Program Studi Farmasi, Fakultas Kedokteran, Universitas Tanjungpura, Pontianakemail : [email protected]/085643182838ABSTRACTGel peel off facial mask is one of the alternative dosage that can improve the comfort to use and is expected to increase antioxidant activity from papaya fruit. Papaya has antioxidant activity due to antioxidant components such as beta-carotene, vitamin C, lycopene. The purpose of this research is to know that methanol extract of papaya fruit can be formulated into gel peel off facial mask preparation, determining polivinyl alkohol (PVA) that used for gelling agent gel peel off facial mask concentration with physical, chemical, and antioxidant activity with the best IC50 value. The method used in the extraction is maceration with methanol solvent. PVA used with concentration are 2,5%; 8,75%; and 17,5%. The Physical test include organoleptic test, adhesion test, spreading test and the chemical test with pH test. Based on the result of the research, it can be concluded that the methanol extract of papaya fruit can be formulated into gel face mask preparation, the formula with PVA concentration of 8,75% fulfill organoleptic rules, with adhesion test 20,16 seconds ± 2,90, spreading test 10,2 cm ± 1,28, pH value 7,13 ± 0,06 with the physical and chemical properties of gel peel off facial mask methanol extract of papaya fruit, and formula with concentration 8,75% PVA has the highest antioxidant activity with IC50 value 80,52 µg/mL that include as strong antioxidant. Keywords: gel peel off facial mask, methanol extract of papaya fruit, PVA, IC50.ABSTRAKMasker gel peel off merupakan salah satu alternatif sediaan yang dapat meningkatkan kenyamanan penggunaan dan diharapkan dapat meningkatkan aktivitas antioksidan buah pepaya. Pepaya memiliki aktivitas antioksidan karena komponen-komponen antioksidan seperti betakaroten, vitamin C, likopen. Tujuan penelitian ini adalah untuk mengetahui bahwa ekstrak metanol buah pepaya dapat diformulasikan menjadi sediaan masker wajah gel peel off dan menentukan konsentrasi polivinyl alkohol (PVA) yang merupakan bahan dasar masker gel peel off yang memiliki sifat fisik, kimia, dan aktivitas antioksidan dengan nilai IC50 terbaik. Metode yang digunakan pada ekstraksi adalah maserasi dengan pelarut metanol. PVA yang digunakan dengan konsentrasi 2,5%; 8,75%; dan 17,5%. Uji sifat fisik meliputi uji organoleptis, uji daya lekat, uji daya sebar, dan uji sifat kimia menggunakan uji pH. Berdasarkan hasil penelitian, diperoleh hasil bahwa ekstrak metanol buah pepaya dapat diformulasikan menjadi sediaan masker wajah gel peel off, formula dengan konsentrasi PVA 8,75% memenuhi persyaratan organoleptik, daya lekat sebesar 20,16 detik ± 2,90, daya sebar sebesar 10,2 cm ± 1,28, nilai pH 7,13 dengan nilai IC50 80,52 µg/mL yang termasuk antioksidan kuat.Kata kunci: masker wajah gel peel off, ekstak metanol buah pepaya, PVA, IC50. 

scholarly journals Aktivitas Antioksidan Fraksi Etil Asetat Dari Ekstrak Metanol Sargassum sp.

2019 ◽  
Vol 8 (1) ◽  
pp. 41-46
Author(s):  
Hafida Salma ◽  
Sri Sedjati Sedjati ◽  
Ali Ridlo
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Vitamin C ◽  
Ethyl Acetate ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Methanol Extract ◽  
Ethyl Acetate Fraction ◽  
Rotary Evaporator ◽  
Total Antioxidant

Sargassum sp. adalah salah satu jenis rumput laut paling banyak di Indonesia dan memiliki manfaat sebagai antioksidan, obat penyakit jantung, stroke, dll. Penelitian ini bertujuan untuk menentukan kandungan antioksidan fraksi etil asetat dari ekstrak metanol Sargassum sp. Sampel diambil dari Pantai Sundak, Gunung Kidul, Yogyakarta. Metode yang digunakan adalah eksploratif diskriptif. Sampel dikeringkan dalam suhu ruangan selama 7 hari dan dimaserasi dengan pelarut metanol, lalu dievaporasi dengan rotary evaporator. Ekstrak metanol di fraksinasi menggunakan etil asetat. Pengujian aktivitas antioksidan dilakukan dengan menggunakan 2 metode, yaitu metode penangkapan radikal bebas DPPH dan total kapasitas antioksidan fosfomolibdat. Vitamin C digunakan digunakan sebagai standar penangkapan radikal bebas DPPH dan total kapasitas antioksidan fosfomolibdat. Kadar total fenolat diuji menggunakan larutan Folin-Ciocalteu dengan standar asam galat. Hasil penelitian menunjukkan bahwa kadar total fenolat fraksi etil asetat Sargassum sp. sebesar 64,42 mg GAE/g sampel. Aktivitas antioksidan dengan metode penangkapan radikal bebas DPPH memiliki nilai IC50 1.289 ppm, vitamin C memiliki IC50 sebesar 122,71 ppm, sedangkan total kapasitas antioksidan adalah 39,52 mg AAE/g sampel. Kesimpulannya, yaitu kandungan aktivitas antioksidan fraksi etil asetat dari ekstrak metanol Sargassum sp. dengan metode penangkapan radikal bebas DPPH diduga sangat lemah dan kandungan aktivitas antioksidan dengan menggunakan metode fosfomolibdat diduga tinggi.  Sargassum sp. is one of the many types of seaweed in Indonesia and has benefits as an antioxidant, a drug for heart disease, stroke, etc. This study aims to determine the antioxidant content of ethyl acetate fraction of methanol extract of Sargassum sp. Samples were taken from Sundak, Gunung Kidul, Yogyakarta. The method used is descriptive explorative. Samples were dried at room temperature for 7 days and macerated with methanol, then evaporated at a rotary evaporator. The methanol extract fractionation using ethyl acetate. Testing the antioxidant activity using two methods, the method of catching free radicals DPPH and total antioxidant capacity fosfomolibdat. Vitamin C is used as a standard arrest DPPH free radical and total antioxidant capacity fosfomolibdat. Levels of total phenolics were tested using the Folin-Ciocalteu solution with gallic acid standard. The results showed that levels of total phenolic fraction of ethyl acetate Sargassum sp. amounting to 64,42 mg GAE/g sample. The antioxidant activity with catching free radicals DPPH methods have IC50 values 1.289 ppm, vitamin C has an IC50 of 122,71 ppm, while the total antioxidant capacity was 39,52 mg AAE/g sample. In conclusion, the content of the antioxidant activity of ethyl acetate fraction of methanol extract of Sargassum sp. with catching free radicals DPPH methods allegedly extremely weak and the content of the antioxidant activity using methods fosfomolibdat predictably high.

scholarly journals Aktivitas Antioksidan Fraksi Dietileter Buah Mangga Arumanis (Mangifera indica L.) dengan Metode DPPH

2017 ◽  
Vol 4 (1) ◽  
Author(s):  
Dewi Andini Kunti Mulangsri ◽  
Aqnes Budiarti ◽  
Endah Novia Saputri
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Diethyl Ether ◽  
Mangifera Indica ◽  
Ethanol Extract ◽  
Natural Antioxidants ◽  
Antioxidant Systems ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ether Fraction

ABSTRAK Sistem antioksidan merupakan bagian dari sistem kekebalan tubuh yang mampu menghambat reaktifitas radikal bebas. Sumber alami antioksidan salah satunya adalah buah yaitu buah mangga Arumanis. Proses fraksinasi dengan pelarut dietileter dilakukan untuk menyari senyawa-senyawa yang bersifat semipolar yang diharapkan memiliki potensi sebagai antioksidan. Penelitian ini bertujuan untuk mengetahui aktivitas antioksidan fraksi dietil eter ekstrak etanol buah mangga arumanis (Mangifera indica L.) dengan Metode DPPH. Ekstraksi dilakukan dengan metode maserasi menggunakan pelarut etanol 70%, kemudian dipartisi cair-cair menggunakan pelarut dietil eter. Penentuan aktivitas antioksidan menggunakan metode DPPH dengan membuat seri konsentrasi fraksi dietil eter ekstrak etanol buah mangga Arumanis yaitu 12,5; 25; 50 dan 100ppm. Vitamin C digunakan sebagai larutan pembanding dengan seri konsentrasi 0,625; 1,25; 2,5 dan 5 ppm. Hasil penelitian menunjukkan bahwa fraksi dietil eter ekstrak etanol buah mangga arumanis memiliki aktivitas antioksidan dengan nilai IC50 sebesar 75,22 ppm dan 1,18 ppm untuk Vitamin C. Fraksi dietileter ekstrak etanol buah mangga Arumanis memiliki potensi lemah sebagai antioksidan jika dibandingkan dengan Vitamin C. Kata Kunci: antioksidan, fraksi dietileter, buah mangga arumanis, Mangifera indica, DPPH ABSTRACT Antioxidant systems are part of the immune system which is able to inhibit the reactivity of free radical. Source of natural antioxidants is a fruit that is mango Arumanis. Process of fractionation with solvent diethylether to extraction the compounds semipolar and expected to have potential as antioxidants. This study aims to determine the antioxidant activity of diethyl ether fraction of ethanol extract Arumanis mango (Mangifera indica L.) with DPPH method. Extraction used ethanol 70% by maceration method, then partitioned using a liquid-liquid partition with diethylether as a solvent. Determination of antioxidant activity using DPPH method with a series of concentrations of diethyl ether fraction of ethanol extract Arumanis mango ie 12.5; 25; 50 and 100ppm. Vitamin C used as a reference solution with a concentration series of 0,625; 1.25; 2.5 and 5 ppm. The results showed that the diethyl ether fraction of ethanol extract Arumanis mango have antioxidant activity with IC50 value of 7.22 ppm and 1.18 ppm for Vitamin C. Diethylether fraction of ethanol extract Arumanis mango has weak potential as a antioxidant, if compared with Vitamin C. Keywords: Antioxidants, diethylether fractions, arumanis mango fruit, Mangifera indica, DPPH.

Antioxidant Activity Of Methanol Extract Of Kesum Leaves (Polygonum Minus Huds.) DPPH MethodAktivitas Antioksidan Ekstrak Metanol Daun Kesum (Polygonum Minus Huds.) Metode DPPH

2018 ◽  
Vol 1 (2) ◽  
pp. 161 ◽  
Author(s):  
Indah Purwaningsih ◽  
Reskiya Sapriani ◽  
Ratih Indrawati
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Methanol Extract ◽  
Beta Carotene ◽  
Polar Compounds ◽  
Antioxidant Compounds ◽  
Dpph Method ◽  
Polygonum Minus ◽  
The Difference ◽  
X 24

Abstract: Kesum Plants (Polygonum minus) is one of the endemic plants in West Borneo containing phenolic compounds, steroids, flavonoids, alkaloids, tannins, saponins, and beta-carotene. Phenolic, flavonoids and beta-carotene are antioxidant compounds. Most phenolic and flavonoid groups are polar compounds. Therefore, in this research used methanol solvent as representative of polar solvent. The purpose of this research  was to know the difference of antioxidant activity of methanol extract of kesum leaves with vitamin C. The extraction  is done by maseration for 3 x 24 hours, where the solvent is changed every 24 hours. The method used to measure antioxidant activity is the DPPH method (2,2-diphenyl-1-picrylhydrazyl). The samples used in this paper are methanol extract of  kesum leaves in 80, 65, 50, 35, and 20 ppm with five replications. The comparator used is vitamin C. The result of the research showed that IC50 of methanol extract was 20,632 ppm and IC50 of vitamin C 6,175 ppm. Based on the results of this study it can be concluded that vitamin C and methanol extract of serum leaves have a very strong antioxidant activity.Abstrak: Tanaman Kesum (Polygonum minus) merupakan salah satu tanaman endemik di wilayah Kalimantan Barat yang mengandung senyawa golongan fenolik, steroid, flavonoid, alkaloid, tanin, saponin, dan betakaroten. Golongan fenolik, flavonoid dan betakaroten merupakan senyawa antioksidan. Sebagian besar golongan fenolik dan flavonoid merupakan senyawa polar. Maka dari itu, pada penelitian ini digunakan pelarut metanol sebagai perwakilan pelarut polar. Tujuan penelitian ini untuk mengetahui perbedaan aktivitas antioksidan ekstrak metanol daun kesum terhadap vitamin C. Proses ekstraksi dilakukan dengan cara maserasi selama 3 x 24 jam, dimana pelarutnya diganti setiap 24 jam sekali. Metode yang digunakan untuk mengukur aktivitas antioksidan adalah metode DPPH (2,2-difenil-1-pikrilhidrazil). Sampel yang digunakan pada penelitian ini yaitu ekstrak metanol daun kesum konsentrasi 80, 65, 50, 35, dan 20 ppm dengan pengulangan sebanyak lima kali. Pembanding yang digunakan adalah vitamin C. Hasil penelitian didapatkan IC50 ekstrak metanol daun kesum sebesar 20.632 ppm dan IC50 vitamin C sebesar 6.175 ppm. Berdasarkan hasil penelitian dapat disimpulkan bahwa vitamin C dan ekstrak metanol daun kesum memiliki aktivitas antioksidan yang sangat kuat.

scholarly journals Antioxidant Activity of Methanol Extract of the Purun Tikus (Eleocharis dulcis) Leaves

2018 ◽  
Vol 3 (3) ◽  
pp. 135 ◽  
Author(s):  
Kholifatu Rosyidah ◽  
Taufiqur Rohman ◽  
Ridhana Fitriani
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Vitamin C ◽  
Methanol Extract ◽  
Open Water ◽  
Extreme Conditions ◽  
Phytochemical Screening ◽  
Strong Activity ◽  
Dpph Method ◽  
Acidic Sulfate

<p>Purun tikus (<em>Eleocharis dulcis</em>) is a kind of swamp plant, can be found in open water areas, both in swamps and rice fields. <em>Purun tikus</em> is an indicator vegetation of acidic sulfate soil. Chemically, plants that able to adapt to special or extreme conditions, usually produce a compound that are unique and have unique bioactivity as well. This paper reports a phytochemical screening of <em>Purun tikus</em> methanol extract and its antioxidant activity. The results showed that the methanol extract of <em>Purun tikus</em> leaves contained phenolic compounds, flavonoids, tannins and terpenoids. By using DPPH method as an antioxidant test, the methanol extract of purun tikus leaves showed a strong activity, with IC<sub>50</sub> value was 58.58 ppm while vitamin C had an IC<sub>50</sub> value of 4.50 ppm.</p>

scholarly journals Aktivitas Antioksidan Ekstrak Metanol Biji Langsat (Lansium domesticum var. pubescens) dan Duku (Lansium domesticum var. domesticum) dengan Metode DPPH

2020 ◽  
Vol 20 (2) ◽  
pp. 113
Author(s):  
Josepin P Konda ◽  
Jainer P Siampa ◽  
Trina E Tallei ◽  
Billy J Kepel ◽  
Fatimawali Fatimawali
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Methanol Extract ◽  
Regression Line ◽  
Seed Extract ◽  
Test Method ◽  
Color Test ◽  
Dpph Method ◽  
Lansium Domesticum ◽  
Positive Results

Penelitian ini bertujuan untuk mengetahui kandungan fitokimia dan aktivitas antioksidan ekstrak biji langsat dan duku dengan metode DPPH. Biji buah langsat dan buah duku diekstraksi menggunakan metode maserasi dengan pelarut metanol. Skrining fitokimia yang dilakukan meliputi uji alkaloid, flavonoid, tannin, triterpenoid, steroid dan saponin secara kualitatif dengan metode uji warna. Uji aktivitas antioksidan menggunakan metode DPPH dan vitamin C sebagai pembanding, serapan diukur menggunakan spektrofotometer UV-Vis pada panjang gelombang 517 nm. Skrining fitokimia pada ekstrak metanol biji langsat menunjukkan hasil positif terhadap alkaloid, triterpenoid dan saponin, sementara pada ekstrak metanol biji duku menunjukkan hasil positif terhadap alkaloid, flavonoid, tannin, triterpenoid, steroid dan saponin. Aktivitas antioksidan ekstrak biji langsat dianalisis menggunakan persamaan garis regresi y = 0,2922x + 33,143, diperoleh IC₅₀ 57,69 μg/mL. Sedangkan aktivitas antioksidan ekstrak biji duku dianalisis menggunakan persamaan garis regresi y = 0.6554x + 29.421, diperoleh IC₅₀ 31,40 μg/mL dan pembanding vitamin C diperoleh IC₅₀ 8,03 μg/mL. Hasil penelitian ini dapat disimpulkan bahwa ekstrak metanol biji buah langsat memiliki kandungan alkaloid, triterpenoid dan saponin serta memiliki aktivitas antioksidan yang kuat sedangkan ekstrak metanol biji buah duku memiliki kandungan alkaloid, flavonoid, tannin, triterpenoid dan saponin serta memiliki aktivitas antioksidan yang sangat kuat.Kata Kunci: Antioksidan, biji duku, biji langsat Antioxidant Activity of Methanol Extract of Langsat Seeds (Lansium domesticum var. pubescens) and Duku Seeds (Lansium domesticum var. domesticum)  by Using DPPH Method ABSTRACTThis study aimed to determine the phytochemical content and antioxidant activity of langsat seed extract and duku seed extract by using the DPPH method. Langsat seeds and duku seeds were extracted by using maceration method with methanol as the solvent. Phytochemical screening was carried out including a qualitative test of alkaloids, flavonoids, steroids/triterpenoids, saponins, and tannins by using the color test method. Antioxidant activity was tested by using the DPPH method and vitamin C used as comparison, the absorption was measured by using UV-Vis spectrophotometer at 517 nm wavelength. The result of phytocemical screening on methanol extract of langsat seeds showed positive results on alkaloids, triterpenoids, and saponins, meanwhile methanol extract of duku seeds showed positive results on alkaloids, flavonoids, triterpenoids, tannin and saponins. The antioxidant activity of methanol extract of langsat seeds was analyzed by using the equation of the regression line y = 0.2922x + 33.143, which obtained IC₅₀ value of 57.69 μg/mL. Meanwhile, the antioxidant activity of methanol extract of duku seeds was analyzed by using the equation of the regression line y = 0.6554x + 29.421, obtained IC₅₀ 31,40 μg/mL. Vitamin C obtained IC₅₀ 8.03 μg/mL. On the basis of all results, it can be concluded that the methanol extract of langsat seeds contained alkaloids, saponins and triterpenoid and has a strong antioxidant activity. Meanwhile, the methanol extract of duku seeds contained alkaloids, flavonoids, triterpenoids, tannin and saponins and has a very strong antioxidant activity.Keywords: Antioxidants, duku seeds, langsat seeds

scholarly journals Antioxidant capacity of snack cookies made from mango and pineapple fermentation

Food Research ◽  
2021 ◽  
Vol 5 (5) ◽  
pp. 145-148
Author(s):  
R. Rompies ◽  
N. Mayulu ◽  
F. Nurkolis ◽  
B.J. Kepel ◽  
H. Natanael ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Mangifera Indica ◽  
Free Water ◽  
Ananas Comosus ◽  
Antioxidant Content ◽  
Three Samples ◽  
Significant Difference ◽  
The Mean

Mango (Mangifera indica) and pineapple (Ananas Comosus) are tropical fruits that contain many nutrients, one of which is antioxidants and polyphenols. Lots of studies have shown that fermented fruit is higher in antioxidants and health benefits. Antioxidants have been reported to be an alternative to enhance body immunity and possibly become an Anti -SARS-CoV-2. One of the antioxidants that are easily found in vitamin C. This study is to process the fermented mango and pineapple fruits into cookies and to test the antioxidant content (especially Vitamin C) in vitro. There were three variations of the formulation, mango: pineapple: CO2 free water. Sample 1 (1: 0.5: 0.5), sample 2 (2: 1: 1) and sample 3 (3: 2: 2). Then, all product samples were inoculated with Lactobacillus paracasei 5% b/v for 14 days under anaerobic conditions to get simpler mangoes and pineapple food fibre. The fermented products were made into flour with a freeze dryer. Sample variation is done to determine the average significance of the antioxidant content in it. The next step was Vitamin C analysis from 3 samples of cookies sample using Titration Iodometric Method, to determine the amount of Vitamin C (mg/100 g) and also the antioxidant activity with 2,2-diphenyl-1-picrylhydrazyl (DPPH). The amount of vitamin C obtained in Sample 1 was 100.20 mg/100 g respectively with antioxidant activity is 35.33%. Sample 2 was 95.75 mg/100 g respectively with antioxidant activity is 30.60%. Sample 3 was 107.90 mg/100 g respectively with antioxidant activity is 44.70%. The formulation with the highest amount of cookies sample containing vitamin C is S3. There was a significant difference (P<0.05) that determined vitamin C levels between sample formulations. The mean ash content of the three samples was 2.02±0.04% and water content were 1.60± 0.15%. The average vitamin C levels in the three sample cookies were 101.28±6.14 mg/100 g. Sample 3 indicated the best antioxidant activity towards 2,2-diphenyl-1- picrylhydrazyl (DPPH) in the amount of 44.70%. Therefore, fermented mango and pineapple have a great potential to be developed into healthy snack cookies. The vitamin C and antioxidants content in cookies from the fermentation of mango and pineapple may be a great substitute for snacks since antioxidants has the ability to improve immunity and anti-inflammatory response. These cookies are also good prebiotics for the gut microbiome which plays a good role in the immune system.

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