Hematological and kidney-functional analysis of acetic acid-induced inflammatory rats administered flavonoid-rich fraction of Ficus sur

2021 ◽  
Vol 1 (1) ◽  
pp. 024-034
Author(s):  
Sunday Adeola Emaleku
Keyword(s):  
Acetic Acid ◽  
Blood Cells ◽  
Wistar Rats ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Pack Cell Volume ◽  
Average Weight ◽  
Blood Disorders ◽  
Experimental Animals ◽  
Functional Assays

Background and aims: Unregulated inflammation causes dysfunctional immune system and could consequently lead to blood disorders due to impaired hematopoietic process, and also destruction of important organs as a result of autoimmunity. In this study, flavonoid-rich fraction of Ficus sur (FRFFS) is investigated for its effects on hematological parameters and renal metabolites in inflammation-induced rats. Materials and methods: Inflammation was induced in Wistar rats of average weight of 112.60 ± 0.75 g by successive oral administration of 6% at (20 ml/kg), 3% at (15 ml/kg) and 6% at (10 ml/kg) acetic acid for one week. Experimental animals were orally administered various doses of FRFFS; 15 mg/kg, 30 mg/kg, 60 mg/kg and 120 mg/kg for two weeks. Blood and kidney samples were collected for hematological parameters and kidney functional assays respectively, and data were analyzed using SPSS version 20. Results: FRFFS significantly (p < 0.05) increased pack cell volume, hemoglobin, red blood cells and platelets, and significantly decreased white blood cells, most especially at 120 mg/kg and 60 mg/kg doses. Similarly, it significantly increased kidney urea and creatinine concentrations. Conclusion: Sequel to these findings, it is inferred that FRFFS could be a potential natural therapy for hematological and renal disorders.

scholarly journals EFFECT OF SILYMARIN EXTRACTED FROM SILYBUM MARIANUM ON NICKEL HEMATOTOXICITY AND NEPHROTOXICITY IN MALE ALBINO WISTAR RATS

2017 ◽  
Vol 9 (8) ◽  
pp. 84
Author(s):  
Samira Bouhalit ◽  
Zine Kechrid ◽  
Abdelfattah Elfeki
Keyword(s):  
Lipid Peroxidation ◽  
Body Weight ◽  
Blood Cells ◽  
Wistar Rats ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Nickel Sulfate ◽  
Enzymatic Antioxidants ◽  
Silybum Marianum ◽  
Renal Markers

Objective: The objective of this study was to investigate the effect of silymarin extract from Silybum marianum against nickel-induced alterations in haematological indices, kidney dysfunction and renal antioxidant defence system.Methods: Male albino Wistar rats were divided into four groups seven each. Control, silymarin, nickel and nickel plus silymarin. Silymarin was administrated orally (100 mg/kg b. wt) and nickel as nickel sulfate (NiSO4 6H20) was given intraperitoneally (20 mg/kg b. wt) at alternative days. The experiment continued for three consecutive weeks. Body weight was recorded regularly. After overnight fasting, animals were killed and serum creatinine, serum urea, serum uric acid, hematological parameters and renal antioxidant markers were determined.Results: The treatment with nickel led to a significant decrease in body weight with an increase in both absolute and relative kidney weights and a significant increase in renal markers, which confirmed by histopathological alteration. A microcytic anemia was also observed, which was manifested by a reduction of red blood cells count (RBC), hemoglobin (Hb) concentration, platelet counts (Plt), hematocrit and white blood cells counts (WBC). The level of lipid peroxidation was increased. Whereas, GSH concentration and enzymatic antioxidants SOD, GSH-Px and CAT activities were decreased. The co-treatment with methanolic extract of milk thistle attenuated the variation in the hematological and renal markers, decreasing renal lipid peroxidation (p<0.05) with a concomitant increasing reduced glutathione content (p<0.01) and restoring the antioxidant enzymes (SOD, CAT, GSH-Px) in kidney, as well as an improvement in histological changes compared to those previously noticed in nickel group.Conclusion: To conclude, these findings demonstrated that silymarin extract effectively improved heamatotoxicity and nephrotoxicity caused by nickel.

Hematological condition of breeding bulls after a long winter period of operation in the conditions of the Udmurt Republic

2022 ◽  
pp. 67-73
Author(s):  
A. Abilov ◽  
A. Azhmyakov ◽  
I. Novgorodova ◽  
N. Bogolyubova
Keyword(s):  
Red Blood Cells ◽  
Reference Values ◽  
Blood Cells ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Winter Period ◽  
Black And White ◽  
Significant Difference ◽  
White Breed ◽  
Udmurt Republic

Purpose: to study hematological parameters of blood in bulls-producers of dairy breeds on the day of semen collection in the Udmurt Republic after a long winter period of operation, depending on the breeds, age and place of selection.Materials and methods. The work was performed at the Federal Research Center for Animal Husbandry named after Academy Member L. K. Ernst on the basis of AO "Udmurtplem" of the Udmurt Republic in the period from 2020 to 2021 on dairy bulls (n=20) aged 15-69 months, including a purebred Holstein breed of domestic selection (n=6), a Holstein black-and-white breed of European selection (the Netherlands, n=6), a black-and-white breed with blood transfusion on Holsteins on at the level of 94-98% (n=8). The content of white blood cells, red blood cells, hemoglobin and hematocrit, depending on age and breed, was studied on the ABC VET hematological analyzer on the day of taking the seed.Results. It was found that on average, in 20 bulls aged 15-69 months, the level of white blood cells was at the level of reference values of 8.8±0.25 x 109/l, red blood cells 10.3 x 1012/l, which is 50% more than the reference values. The concentration of hemoglobin is 128.0±2.92 g/l, hematocrit is on average 54.3%, with a norm of 24-46%. Breeding bulls at a reliable level, differing in age, showed that some animals had high indicators for red blood cells of 10.3±0.26 x 102, for hematocrit of 54.3% against 24-46% of reference values. Also, according to hematological indicators, there was a tendency to increase the concentration of hemoglobin and hematocrit in European-bred bulls.Conclusion. The study of the variability in hematological parameters depending on the selection showed that there is no significant difference in leukocytes and all indicators are at the level of reference values, and in erythrocytes more than 50% than the highest indicators. The highest hemoglobin values were at the level of 141-156 g/l instead of 128 g/l according to the highest reference values. Hematocrit also showed high max values in all groups in comparison with the reference values of 59-66% versus 46% in the norm. It is necessary when analyzing hematological blood parameters in addition to the average statistical indicators (M+m) also, monitor the variability (min-max) in order to obtain more objective information.

scholarly journals Impact complexes nanokarboksylativ and drug Kvatronan-Se on hematological parameters of blood of experimental animals

2017 ◽  
Vol 19 (74) ◽  
pp. 123-126
Author(s):  
N. B. Seba ◽  
M. A. Khomenko
Keyword(s):  
Blood Cells ◽  
Hematological Parameters ◽  
Live Weight ◽  
Control Group ◽  
Experimental Animals ◽  
Group I ◽  
Control Research ◽  
Research Experiment ◽  
The Village ◽  
Experimental Group

In this paper present the results of research of influence of the preparation «Kvatronan-Se» and carboxylates of Se, Cu, Mn, Cr, Ge in different combinations on haematological parameters of blood of experimental animals. Study was carried out on the basis of PC Haleks-Agro. It is located in the village Gul'sk of Novograd Volynskiy district of Zhytomyr region. In the farm to bred of Simmental cows. For the experiment was formed four groups. We took into account age, live weight of 650–700 kg and the average milk yield 5000–6000 kg. Animals is located in identical conditions of feeding and maintenance. Research experiment was carried out on animals couples counterparts.  Preparations introduced animals three times at 10–12 days after insemination. The cows first experimental group were injected complex nanokarboksylativ Ge, Cu, Mn and Cr, second – drug Kvatronan–Se and animals third experimental group was administered a complex composed of Se, Cu, Mn, Cr. On 13 day after insemination in experimental animals were taken blood from tail vein and examined the contents of hematological parameters. Established that injection nanokarboksylativ enhance the fertility of cows in the first and third group, the figure rose to 20% in the second by 40% compared with the control. Research hematological parameters blood  animals shows that after entering content drugs of blood cells had minor changes but remained within the physiological norm. The results show that in  concentration monocyte the second group  was at 5.53% (P > 0.01) higher compared to the control group and 2.3% compared to the first and third. The content of lymphocytes in this group decreased by 26.3% (P > 0.05), 22% and 16% relative to the control, the first and third groups. Also, it should be noted that the hemoglobin in the experimental groups decreased in group I by 7.9% in the II – 3.9% and III by 7.3% compared with the control group. Also, these groups distinguished downward trend eosinophils 1.8%, 1.5%, 2.05%. Then, as the number of segment-nuclear neutrophils contrast, in the first group increased by 18.2% in the second and third at 16.9% comparatively control. The number of monocytes in the second group doslfidniy the suspicion was higher by 66.3% and 0.3% higher than the physiological norm – it may indicate a high immune response.

scholarly journals In-vivo Antiplasmodial Effect of Dihydroartemisinin-Piperaquine-Nitrofurantoin on Plasmodium berghei- Infected Mice

2021 ◽  
pp. 12-20
Author(s):  
Udeme O. Georgewill ◽  
Festus Azibanigha Joseph ◽  
Elias Adikwu
Keyword(s):  
Plasmodium Berghei ◽  
Blood Cells ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Positive Control ◽  
Negative Control ◽  
Antiplasmodial Activity ◽  
Significant Difference ◽  
Tract Infections

Nitrofurantoin (NT) used for the treatment of urinary tract infections may have antiplasmodial activity. Dihydroartemisinin-piperaquine (DP) is an artemisinin based combination therapy used for the treatment of malaria. This study evaluated the antiplasmodial effect of dihydroartemisinin-piperaquine-nitrofurantoin (DP-NT) on mice infected with Plasmodium berghei. Adult Swiss albino mice (30-35 g) of both sexes were used. The mice were randomly grouped, inoculated with Plasmodium berghei, and treated orally with DP (1.7/13.7 mg/kg), NT (57.1 mg/kg) and DP-NT (1.71/13.7/ 57.1 mg/kg), respectively using curative, prophylactic and suppressive tests. The negative control was orally treated with normal saline (0.3 mL), while the positive control was orally treated with chloroquine CQ (10mg/kg). After treatment, blood samples were collected and evaluated for percentage parasitemia, inhibitions and hematological parameters. Liver samples were evaluated for histological changes. The mice were observed for mean survival time (MST). Treatment with DP-NT decreased parasitemia levels when compared to individual doses of DP and NT with significant difference observed at p<0.05. DP-NT prolonged MST when compared to individual doses of DP and NT with significant difference observed at p<0.05. The decrease in packed cell volume, red blood cells, hemoglobin and increase in white blood cells in parasitized mice were significantly restored by DP-NT  when compared to individual doses of DP and NT with difference observed at p<0.05. DP-NT eradicated liver Plasmodium parasite.  NT remarkably increased the antiplasmodial activity of DP. DP-NT may be used for the treatment of malaria.

scholarly journals Evaluation of the hemogram of Oryctolagus cuniculus envenomus

2020 ◽  
Vol 13 (2) ◽  
pp. 266-272
Author(s):  
Obou Constantin Okou ◽  
N’guessan Emmanuel Assemian ◽  
Kouadio Bernard Allali ◽  
Guy Childeric Bingo ◽  
Allico Joseph Djaman
Keyword(s):  
Blood Cells ◽  
Oryctolagus Cuniculus ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Experimental Batch ◽  
Haematological Parameters ◽  
Rabbit Blood ◽  
Hemolysis Test ◽  
Dose Dependent

The overall objective of this study was to evaluate the hemolysing action of Naja nigricollis venom on rabbit blood. To carry out this study, three batches of three rabbits were formed with two control batches and one experimental batch. Each control lot is composed of three rabbits (males or females) while the experimental lot is composed of two males and one female. Each rabbit from the control lots was separately collected in the purple tube (EDTA) and transported to the laboratory for analysis. The rabbits from the experimental batch were also collected distinctly a few minutes after the injection of the venom of Naja nigricollis for the analysis of haematological parameters. However, before the analysis of the hematological parameters of the rabbits from the control and experimental batches, an in vitro hemolysis test of Naja nigricollis venom was performed to verify its hemolysing power. The results showed that Naja nigricollis venom has a dose-dependent in vitro hemolysing power. As for the haemogram, it revealed that the venom of Naja nigricollis has a decreasing effect on blood cells (red and white blood cells), on haemoglobin and on haematocrit, and an elevation on MGVs thus promoting anaemia.

Exploring the therapeutic potential of apple extracts against thrombocytopenia in rat experimental models

2018 ◽  
Vol 48 (3) ◽  
pp. 397-405
Author(s):  
Munawar Abbas ◽  
Farhan Saeed ◽  
Muhammad Umair Arshad ◽  
Muhammad Tahir Nadeem ◽  
Huma Bader-Ul-Ain ◽  
...  
Keyword(s):  
Platelet Count ◽  
Blood Cells ◽  
Therapeutic Potential ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Apple Fruit ◽  
Antioxidant Compounds ◽  
Sprague Dawley ◽  
Content Type ◽  
Apple Seed

Purpose This paper aims to evaluate the therapeutic potential of apple extracts against thrombocytopenia, i.e. decrease in platelet count. Design/methodology/approach Male Sprague Dawley rats were used to evaluate therapeutic potential of apple extracts. Diets enriched with apple fruit and seed’s ethanolic extract were provided to normal and KBrO3-induced thrombocytopenic rats for 60 days. KBrO3 was administered at level of 85 mg/Kg of body weight of rats to intentionally decrease the platelet count. Platelet count and other hematological parameters were monitored at monthly intervals to evaluate therapeutic effect of apple extracts against thrombocytopenia. Findings The results of current research portrayed that both apple seed and fruit extracts enriched diets increased the platelet count significantly (p < 0.05) in thrombocytopenic rats. It was observed that apple fruit extract-enriched diet (AFE) raised the platelet count to 14.72 and 33.07 per cent in normal and thrombocytopenic rats, respectively. Apple seed extract-enriched diet (ASE) raised the platelet count to 11.08 and 32.90 per cent in normal and thrombocytopenic rats, respectively. Other hematological parameters including white blood cells, red blood cells and hemoglobin were also significantly increased. Originality/value Thrombocytopenia is uprising problem in developing world including India and Pakistan accompanied by different diseases especially dengue and related complications. Because of questioning on therapies against thrombocytopenia, research on diet-based therapies, as a substitute to synthetic one, is increasing. Diet rich in antioxidant compounds including apple fruit and seeds are the limelight of manuscript.

scholarly journals T lymphocyte subsets of the umbilical cord blood of dogs

2009 ◽  
Vol 61 (4) ◽  
pp. 791-796 ◽  
Author(s):  
M.L.B. Cápua ◽  
A.E. Santana ◽  
A.P.M. Nakage ◽  
A.V. Godoy ◽  
A. Kataoka
Keyword(s):  
Cord Blood ◽  
Umbilical Cord ◽  
T Lymphocyte ◽  
Umbilical Cord Blood ◽  
Blood Cells ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Hemoglobin Concentration ◽  
Cd4 Cells ◽  
T Lymphocyte Subsets

The hematological parameters red blood cells (RBC) and total white blood cells (WBC) counts, hematocrit, hemoglobin concentration, and RBC indexes (median corpuscular volume and median corpuscular hemoglobin concentration) were determined and T CD5+ lymphocytes and CD4+ and CD8+ subpopulations of the umbilical cord blood (UCB) of dogs were quantified by the cytofluorimetric technique. Nine adult Beagles, from two do five-year old, were used as control. The umbilical cord blood (UCB) was collected from 20 neonate dogs. The method for the UCB collection was adequate to obtain sufficient quantity of blood for the accomplishment of the hematological analyses and lymphocyte quantification. Cytoscopic preparations of the UCB suggested high erythropoietic activity. There was no difference for the global leukocyte and lymphocyte counts between the groups. UCB T lymphocyte counts were lower than those obtained for adult dogs. The proportion of CD4:CD8 showed a great dominance of T CD4+ cells over T CD8+ lymphocytes in UCB.

scholarly journals Breakfast can Affect Routine Hematology and Coagulation Laboratory Testing: An Evaluation on Behalf of COLABIOCLI WG-PRE-LATAM

TH Open ◽  
2019 ◽  
Vol 03 (04) ◽  
pp. e367-e376 ◽  
Author(s):  
Maria Elena Arredondo ◽  
Eduardo Aranda ◽  
Rubén Astorga ◽  
Lorena Michele Brennan-Bourdon ◽  
Marise Danielle Campelo ◽  
...  
Keyword(s):  
Latin American ◽  
Health Care Professionals ◽  
Partial Thromboplastin Time ◽  
Blood Cells ◽  
Laboratory Testing ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Activated Partial Thromboplastin Time ◽  
Coagulation Testing ◽  
Fasting Time

AbstractLaboratories worldwide perform both hematological and coagulation testing on patients avoiding fasting time. In 2017, the Latin America Confederation of Clinical Biochemistry (COLABIOCLI) commissioned the Latin American Working Group for Preanalytical Phase (WG-PRE-LATAM) to study preanalytical variability and establish guidelines for preanalytical procedures to be applied by clinical laboratories and health care professionals. This study, on behalf of COLABIOCLI WG-PRE-LATAM, aims to evaluate the effect of the breakfast on routine hematology and coagulation laboratory testing. We studied 20 healthy volunteers who consumed a breakfast containing a standardized amount of carbohydrates, proteins, and lipids. We collected blood specimens for routine hematology and coagulation laboratory testing before breakfast and 1, 2, and 4 hours thereafter. Significant differences between samples were assessed by the Wilcoxon ranked-pairs test. Statistically significant differences (p < 0.05) between basal and 4 hours after the breakfast were observed for red blood cells, hemoglobin, hematocrit, mean corpuscular volume, white blood cells, neutrophils, lymphocytes, monocytes, mean platelet volume, and activated partial thromboplastin time. In conclusion, the significant variations observed in several hematological parameters, and activated partial thromboplastin time due to breakfast feeding demonstrate that the fasting time needs to be carefully considered prior to performing routine hematological and coagulation testing to avoid interpretive mistakes of test results, and to guarantee patient safety. Therefore, COLABIOCLI WG-PRE-LATAM encourages laboratory quality managers to standardize the fasting requirements in their laboratory, i.e., 12 hours.

scholarly journals Changes in morphology of white blood cells on peripheral smear in COVID-19 infection

2021 ◽  
Vol 9 (8) ◽  
pp. 2393
Author(s):  
Garima Agarwal ◽  
Shubhangi Gupta ◽  
Natasha Singh ◽  
Salony Mittal ◽  
Atul Verma ◽  
...  
Keyword(s):  
Infectious Disease ◽  
Blood Cells ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Peripheral Smear ◽  
First Case ◽  
The World ◽  
Blue Stain ◽  
Coronavirus Infection ◽  
Made In

Background: COVID-19 is an infectious disease caused by a newly discovered coronavirus, and has spread around the world in a deadly pandemic. The first case of COVID-19 was reported from Wuhan, China in December 2019. This is also called as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) because of its homology with SARS virus. The most common hematological manifestation of coronavirus is lymphopenia which is due to depletion of lymphocytes by coronavirus infection. Other manifestations are neutrophilia and mild thrombocytopenia. Literature is full of quantitative hematological parameters but the researches on morphology of white blood cells is still ongoing. We at our institute done study on 60 confirmed positive cases of COVID-19, and analyzed those peripheral smears in terms of morphology of white blood cells.Methods: The study was done using peripheral smear staining with methylene blue stain and was screened for various changes in white blood cells in peripheral smear.Results: Changes in the white blood cells were examined in the peripheral smear and findings were made in the tabular form.Conclusions: To conclude that all these changes are due to the virus infecting them or are secondary to pathogenesis of COVID disease, needs to be evaluated by larger studies.

scholarly journals Bioefficiency of Chromolaena odorata (Linn.) on hematological and lipid profiles in sublethal cyanide poisoning in male wistar rats

2020 ◽  
Vol 12 (1) ◽  
pp. 13-18
Author(s):  
Adeola. Folashade Ehigie ◽  
Gbadebo Emmanuel Adeleke ◽  
Fiyinfoluwa. D. Ojeniyi ◽  
Leonard Ona Ehigie
Keyword(s):  
Lipid Profile ◽  
Blood Cells ◽  
Wistar Rats ◽  
Hematological Parameters ◽  
Chromolaena Odorata ◽  
Sodium Thiosulphate ◽  
Cyanide Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
The Impact

Chromolaena odorata is an invasive plant which is acclaimed to have cyanide remediation potential from contaminated sites. This examination means to decide the impact of ethanol concentrates of C. odorata (ECO), sodium thiosulphate and a mix of both on hematological parameters and blood lipid profile of rodents presented to potassium cyanide. (KCN). A sum of thirty five male Wistar rats partitioned into seven groups of five units were used. KCN Group rats were administered with KCN alone. Rats in 100ECO, 150ECO, 200ECO groups were administered with 100, 150 and 200 mg/kg body weight of ECO respectively. Rats under Na2sS2O3 and Na2S2O3+ECO groups were administered 200 mg/kg sodium thiosulphate and sodium thiosulphate with ECO at 200 mg/kg respectively. The trial was done in about a month. Toward the finish of the investigation, the packed cell volume (PCV), hemoglobin level (Hb), Red blood cells (RBC) and white blood cells (WBC) were resolved utilizing known biochemical methodology. The outcomes demonstrated a noteworthy increment (p ? 0.05) in PCV, Hb, RBC and WBC level of remedial groups when contrasted with the cyanide group. Total cholesterol (TC), and triglyceride (TG) were altogether lower while HDL-cholesterol was fundamentally expanded in all the treated groups when contrasted and the untreated group given cyanide alone. However, no significant difference in LDL-cholesterol was indicated in all therapeutic groups compared with the cyanide group. The study revealed that C. odorata at the tested doses was able to improve the hematological parameters and lipid profile in cyanide exposed rats.

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