The Influence of Lunar Phase on the Germination of Creole Corn in a Controlled Environment

Although the influence of lunar phase on plant growth and development has been disregarded by most researchers, there is still little data in the literature regarding the influence of lunar phase within a controlled environment. Objective evaluates the influence of lunar phase on the germination of creole corn and on seedling development in a controlled environment. The experimental design was completely randomized with the following treatments: four lunar phases (full, waxing, waning and new), with four replications totaling 50 seeds per treatment. The treatments started on the first day of the waxing phase and extended to the full phase within the same month, respecting the germination period (nine days) and the length of each moon phase (roughly seven days). The following parameters were evaluated: germination rate, seedling length, fresh and dry weight of shoots and roots. Seedlings germinated during the waning lunar phase were longer and had greater fresh root weight and greater fresh and dry shoot weights than did seedlings germinated during the other phases. However, some limiting factors (long term experiments and in different places in the world) must be considered in future research to determine whether moon phase affects germination. Thus, longer-term, controlled, and modeled studies are needed to analyze external factors, which may influence the germination of cultivar seeds.

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The efficacy of different seed priming agents for promoting sorghum germination under salt stress

PLoS ONE ◽

10.1371/journal.pone.0245505 ◽

2021 ◽

Vol 16 (1) ◽

pp. e0245505

Author(s):

Xiaofei Chen ◽

Ruidong Zhang ◽

Yifan Xing ◽

Bing Jiang ◽

Bang Li ◽

...

Keyword(s):

Salt Stress ◽

Seed Germination ◽

Germination Rate ◽

Principal Component ◽

Dry Weight ◽

Seed Priming ◽

Nacl Stress ◽

Future Research ◽

Fresh Weight ◽

Positive Effects

Sorghum [Sorghum bicolor (L.) Moench] seed germination is sensitive to salinity, and seed priming is an effective method for alleviating the negative effects of salt stress on seed germination. However, few studies have compared the effects of different priming agents on sorghum germination under salt stress. In this study, we quantified the effects of priming with distilled water (HP), sodium chloride (NaCl), potassium chloride (KCl), calcium chloride (CaCl2), and polyethylene glycol (PEG) on sorghum seed germination under 150 mM NaCl stress. The germination potential, germination rate, germination index, vigor index, root length, shoot length, root fresh weight, shoot fresh weight, root dry weight, and shoot dry weight were significantly reduced by salt stress. Different priming treatments alleviated the germination inhibition caused by salt stress to varying degrees, and 50 mM CaCl2 was the most effective treatment. In addition, the mitigation effect of priming was stronger on root traits than on shoot traits. Mitigation efficacy was closely related to both the type of agent and the concentration of the solution. Principal component analysis showed that all concentrations of CaCl2 had higher scores and were clearly distinguished from other treatments based on their positive effects on all germination traits. The effects of the other agents varied with concentration. The priming treatments were divided into three categories based on their priming efficacy, and the 50, 100, and 150 mM CaCl2 treatments were placed in the first category. The 150 mM KCl, 10% PEG, HP, 150 mM NaCl, 30% PEG, and 50 mM KCl treatments were placed in the second category, and the 100 mM NaCl, 100 mM KCl, 20% PEG, and 50 mM NaCl treatments were least effective and were placed in the third category. Choosing appropriate priming agents and methods for future research and applications can ensure that crop seeds germinate healthily under saline conditions.

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Establishment of evaluation procedure for soybean seed-flooding tolerance and its application to screening for tolerant germplasm sources

Legume Research - An International Journal ◽

10.18805/lr.v0iof.9112 ◽

2017 ◽

Author(s):

Muhammad Jaffer Ali ◽

Zheping Yu ◽

Guangnan Xing ◽

Tuanjie Zhao ◽

Junyi Gai

Keyword(s):

Germination Rate ◽

Soybean Seed ◽

Petri Dish ◽

Dry Weight ◽

Testing Procedure ◽

Evaluation Procedure ◽

Breeding Lines ◽

Seedling Length ◽

Relative Root Length ◽

Relative Root

The experiments concluded 48hrs for germination testing as most appropriate stress duration. Using paper roll for recording the traits was the best method than petri dish and towel method. Among the traits relative germination rate (RGR), relative seedling length (RSL), relative root length (RRL), relative root fresh weight (RRFW) and relative root dry weight (RRDW) were proved to be flooding-responsive. RSL was chosen as the major seed-flooding indicator due to higher heritability (h2), genotypic coefficient of variation (GCV), correlation with other indicators and easier measuring procedure, while RRL was considered as the subsidiary indicator. Thus, the RSL in paper roll after 48hrs seed-flooding was recognized as standard seed-flooding testing procedure and used to evaluate breeding materials. Among the 11 cultivars, the superior seed-flooding-tolerant ones were M8206, NN1138-2 and ZXD, while ten best lines were evaluated from one hundred breeding lines.

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Accelerated Aging Test to Determine the Vigor of Mungbean Seeds

Journal of Agricultural Science ◽

10.5539/jas.v11n7p42 ◽

2019 ◽

Vol 11 (7) ◽

pp. 42

Author(s):

Luciana Maria da Silva ◽

Anderson Dias Vaz de Souza ◽

Rogério Lámim Silva Junior ◽

Natália Arruda ◽

Nei Peixoto ◽

...

Keyword(s):

Seed Quality ◽

Germination Rate ◽

Accelerated Aging ◽

Dry Weight ◽

Precise Determination ◽

Germination Test ◽

Accelerated Aging Test ◽

Seedling Length ◽

Aging Test ◽

Randomized Experimental Design

The use of good quality seeds is fundamental for proper establishment of a crop. In this way, for more precise determination of seed quality, vigor tests are performed in addition to the germination test. These tests enable the selection of the best lots for commercialization and planting. This study evaluates the effects of temperature and exposure times of the accelerated aging test for vigor classification of mungbean seed lots. Seeds of the mungbean cultivar Esmeralda were used, being obtained from four cultivated plots at the State University of Goiás (UEG), Ipameri Câmpus, in the 2013, 2015, 2016, and 2017 harvests. The lots were initially characterized using germination and vigor tests (first germination count, germination rate index, seedling length, and seedling fresh and dry weight). The accelerated aging test was conducted with a completely randomized experimental design, in a 2 × 4 factorial arrangement consisting of two temperatures (40 and 42 °C) and four times (24, 48, 72, and 96 hours), with four replicates of 50 seeds per lot. The lots showed significant differences in the germination test, first germination count, germination rate index, and seedling length. The accelerated aging test was efficient in classifying lots. Lot 3 obtained the best results, while lot 1 obtained the lowest ones. The combination of 42 °C temperature and 72 h of seed exposure to the accelerated aging test is the best to classify mungbean seed lots.

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The Hormonal Seed Priming in Relation to Carrot Germination

Journal La Lifesci ◽

10.37899/journallalifesci.v1i6.261 ◽

2020 ◽

Vol 1 (6) ◽

pp. 1-5

Author(s):

Filza Ghafoor ◽

Sana Liaqat ◽

Waseem Iqbal

Keyword(s):

Root Length ◽

Dry Matter ◽

Germination Rate ◽

Dry Weight ◽

Seed Priming ◽

Matter Content ◽

Dry Matter Content ◽

Fresh Weight ◽

Stress Effects ◽

Seedling Length

Seed priming is a pre-sowing treatment used to reduce stress effects in crops .This technique has been used to increase the germination rate of seeds mainly under unfavorable environmental condition. Crop productivity faces many problems like that unavailability of suitable condition that causes unbalanced seedling growth and poor germination. Hormones are essential to improve plant growth, development, crop yield quality and quantity. Auxin is essential hormones that improves germination of seeds and reduce stress effects. Laboratory experiment was conducted to study the effect of seed priming using different concentrations of IAA and IBA on carrot seeds (Daucus carota). In laboratory experiment, three concentrations of IAA and IBA (75 ppm, 50ppm, 25ppm) treated with distilled water with priming duration of 24 hours. Experiment was laid out in CRD with three replications. Data was collected to investigate the effect of seed priming with IAA and IBA under these condition like that (fresh weight, dry weight, speed of germination, root length, seed length, seedling length, final germination rate, mean germination time and dry matter content). Maximum fresh weight, final germination percentage, seedling length, energy of germination, speed of germination and mean germination rate was recorded IBA 75ppm. Maximum dry weight and dry matter content was observed IBA 25ppm.Maximum shoot length was recorded IBA 50ppm. Non-significant difference was recorded in root length.

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Identifying morphological traits associated with vegetative persistence in the perennial ryegrass (Lolium perenne L.) cultivar 'Grasslands Samson'

Journal of New Zealand Grasslands ◽

10.33584/jnzg.2020.82.434 ◽

2020 ◽

Vol 82 ◽

pp. 139-147

Author(s):

Jessica R. O'Connor ◽

James R. Crush ◽

Zulfi Jahufer

Keyword(s):

Lolium Perenne ◽

Perennial Ryegrass ◽

Morphological Traits ◽

Genotypic Variation ◽

Dry Weight ◽

Tiller Number ◽

Future Research ◽

Lolium Perenne L ◽

Genetic Shift

Perennial ryegrass (Lolium perenne L.) vegetative persistence (maintained herbage growth and survival without reseeding) is an important economic trait for farmers in New Zealand as it decreases the frequency of reseeding pastures. Vegetative persistence is difficult to breed for due to a lack of long-term trials to observe the complex interactions between plant genotype and the environment. In a long-term trial a genetic shift in the sown cultivar population could occur as individual plants with advantageous traits outcompete other plants and survive. The objectives of our study were to investigate the occurrence of a potential genetic shift in a sample (30 plants) of a persistent population of ‘Grasslands Samson’ perennial ryegrass. Persistent plants were collected from a nine-year-old trial at Poukawa, Hawke's Bay. To identify a genetic shift, these plants were compared to a sample of 30 plants sourced from commercial seed of ‘Grasslands Samson’ representing the original population. This study estimated genotypic variation within and between the populations for eight morphological traits after 10 weeks' growth under glasshouse conditions. Phenotypic and genotypic correlations between the traits were estimated. Results showed that the persistent population had significantly (P<0.05) greater means for tiller number, reproductive tiller number, lamina sheath length, and dry weight. Future research studying these traits across farm environments would further understanding of their roles in vegetative persistence of ryegrass.

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Effect of seed priming with kinetin on germination indices and proline activity of Silybum marianum L. under drought stress conditions

International Journal of Life Sciences ◽

10.3126/ijls.v9i1.11919 ◽

2015 ◽

Vol 9 (1) ◽

pp. 6-11

Author(s):

Abolfazl Masoumi Zavariyan ◽

Mojtaba Yousefi Rad ◽

Mohsen Asghari

Keyword(s):

Drought Stress ◽

Germination Rate ◽

Dry Weight ◽

Seed Priming ◽

Germination Percentage ◽

Seed Vigor ◽

Silybum Marianum ◽

Randomized Design ◽

Seedling Length ◽

Germination Indices

For investigation of the effect of seed priming by kinetin on germination indices and proline activity of Silybum marianum L. under drought stress, factorial experiment based on completely randomized design with two factor includingdifferent concentrations of kinetin (0, 10 and 20 ppm) and different levels of drought stress including 0, -6 and -12 barat three replicates was carried out. According to the results, drought stress and kinetin individualy cause to decreases of germination percentage, germination rate, seedling length, seed vigor and seedling dry weight but uses of kinetin in drought stress condition improvment of them. Also, drought stress cause to increases of proline content and catalase activity. Therefore, seed priming with 10 ppm kinetin at drought stress was improved the germination indices of Silybum marianum L.DOI: http://dx.doi.org/10.3126/ijls.v9i1.11919 International Journal of Life Sciences Vol.9(1) 2015 6-11

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Priming treatments for improvement of germination and emergence of cotton seeds at low temperature

Plant Breeding and Seed Science ◽

10.1515/plass-2015-0027 ◽

2015 ◽

Vol 71 (1) ◽

pp. 121-134 ◽

Cited By ~ 6

Author(s):

Hatice Çokkizgin ◽

Yüksel Bölek

Keyword(s):

Cold Stress ◽

Aminolevulinic Acid ◽

Germination Rate ◽

Dry Weight ◽

Hypocotyl Length ◽

Seed Vigour ◽

Seedling Length ◽

Cotton Genotypes ◽

Good Stand ◽

Radicle Length

AbstractEarly sowing in cotton is important for obtaining a high and qualiy yield. Since cotton prefers high temperatures to have a good stand establisment, this work is important to determine better performing genotypes or to promote germination and emergence at low temperatures (<20°C). This research aimed to increase seed germination rate and seed vigour under cold stress conditions. Two cotton genotypes belonging toG. hirsutumL. species (Aydin-110 and Paymaster-2379) were primed with PEG-6000 (200, 300, 400 g/L), KNO3(2, 3, 4%), KH2PO4(0.3, 0.4, 0.5 M), NaCl (2, 3, 4%) or Mannitol (2, 4, 6%) to determine the best priming medium and its best amount. After priming, seeds were germinated in germination cabin at cold (18°C) and warm (30°C) temperatures and some characteristics (germination rates, radicle length, hypocotyl length and seedling length) were analyzed. Priming with KNO3(4% or 2%) was found to result in the highest germination rates in both cotton genotypes. The seeds of the genotypes were also primed with 5-aminolevulinic acid (ALA) (1, 10, 25 mg/L), gibberellic acid (GA3) (100, 250, 500 μM), methyl jasmonate (MeJA) (1, 3, 5 μM), acetylsalicylic acid (ASA) (1, 10, 100 μM) or kinetin (1, 5, 10 mg/L) added to KNO3(4%) medium to choose the most effective plant growth regulator (PGR) application and its dose. PGRs were observed to have reverse effects on germination rate. Finally, emergence test was conducted with KNO3(4%) and KNO3(2%) using metabolic chill test, and some traits (radicle length, radicle weight, hypocotyl length, hypocotyl weight, seedling length, seedling fresh weight and seedling dry weight) were examined. Our results suggested that priming with KNO3(2%) might be an effecient way to have vigourous cotton plants under the cold stress.

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Haplotype Analysis of BADH1 by Next-Generation Sequencing Reveals Association with Salt Tolerance in Rice during Domestication

International Journal of Molecular Sciences ◽

10.3390/ijms22147578 ◽

2021 ◽

Vol 22 (14) ◽

pp. 7578

Author(s):

Myeong-Hyeon Min ◽

Thant Zin Maung ◽

Yuan Cao ◽

Rungnapa Phitaktansakul ◽

Gang-Seob Lee ◽

...

Keyword(s):

Salt Stress ◽

Salt Tolerance ◽

Plant Traits ◽

Germination Rate ◽

Dry Weight ◽

Future Research ◽

Betaine Aldehyde Dehydrogenase ◽

Cultivated Rice ◽

Germination Time ◽

Mean Germination Time

Betaine aldehyde dehydrogenase 1 (BADH1), a paralog of the fragrance gene BADH2, is known to be associated with salt stress through the accumulation of synthesized glycine betaine (GB), which is involved in the response to abiotic stresses. Despite the unclear association between BADH1 and salt stress, we observed the responses of eight phenotypic characteristics (germination percentage (GP), germination energy (GE), germination index (GI), mean germination time (MGT), germination rate (GR), shoot length (SL), root length (RL), and total dry weight (TDW)) to salt stress during the germination stage of 475 rice accessions to investigate their association with BADH1 haplotypes. We found a total of 116 SNPs and 77 InDels in the whole BADH1 gene region, representing 39 haplotypes. Twenty-nine haplotypes representing 27 mutated alleles (two InDels and 25 SNPs) were highly (p < 0.05) associated with salt stress, including the five SNPs that have been previously reported to be associated with salt tolerance. We observed three predominant haplotypes associated with salt tolerance, Hap_2, Hap_18, and Hap_23, which were Indica specific, indicating a comparatively high number of rice accessions among the associated haplotypes. Eight plant parameters (phenotypes) also showed clear responses to salt stress, and except for MGT (mean germination time), all were positively correlated with each other. Different signatures of domestication for BADH1 were detected in cultivated rice by identifying the highest and lowest Tajima’s D values of two major cultivated ecotypes (Temperate Japonica and Indica). Our findings on these significant associations and BADH1 evolution to plant traits can be useful for future research development related to its gene expression.

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PENGGUNAAN MEDIA ALTERNATIVE (DIAPERS) TERHADAP PERTUMBUHAN DAN PRODUKSI TANAMAN CABAI

Jurnal Agrium ◽

10.29103/agrium.v15i1.687 ◽

2018 ◽

Vol 15 (1) ◽

pp. 34

Author(s):

Safrizal Safrizal ◽

Nazimah Nazimah ◽

Rina Resssi

Keyword(s):

Dry Weight ◽

Leaf Length ◽

Household Waste ◽

Home Garden ◽

Root Weight ◽

Plant Canopy ◽

Number Of Leaves ◽

Different Response

Red chili is a major vegetable both in Indonesia and abroad due to its benefits to the needs of nutrition and health through cooking spices. Utilization of home garden yard is an effective way to cultivate pepper plants. On the other hand, every household produces household waste which is difficult to decompose for instance diapers, so that it can cause environmental pollution in long-term. This study was conducted to investigate the role of planting media such as diapers of on chili growth. The results showed that the use of diapers of diapers as planting media gave no significant different to plant height at 1, 4, 7, 10, 13 and 16 week after application. It also did not increase the number of leaves at the age of 1, 7, 10, 13 and 16 week after application. There were no changes in leaf length at ages 1, 4, 7, 10 and 16 week after application. It also happened to also its leaf width at ages 1, 4, 7, 10, 13 and 16 week after application and number of branches at ages 1, 4, 7, 13 and 16 week after aplication. However, it showed a significant different to the number of leaves at age 4 week after planting and its leaf length at age 13 week after planting. The interaction of using diapers as planting media gave significantly different response to root number, root length, wet root weight and plant canopy, and dry weight roots and plant canopy.

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Assessment of long term interaction between the endemic seagrass P. oceanica and the exotic green alga C. cylindracea in the South Eastern coast of Spain (Murcia Region)

10.7287/peerj.preprints.1065 ◽

2015 ◽

Author(s):

Jaime Bernardeau-Esteller ◽

Rocio García-Muñoz ◽

Lázaro Marín-Guirao ◽

Jose Miguel Sandoval-Gil ◽

Ruiz Juan Fernandez

Keyword(s):

Green Alga ◽

Algal Growth ◽

Interactive Effects ◽

Limiting Factors ◽

Eastern Coast ◽

Future Research ◽

The South ◽

South Eastern ◽

Leaf Canopy

The green alga Caulerpa cylindracea has rapidly spread throughout the Western Mediterranean during the last 20 years. Compared with other native benthic communities, Posidonia oceanica meadows seems to be highly resistant to the colonization by the alga. Nonetheless, it is suggested that in the long-term C. cylindracea could affect the seagrass altering its vitality, structure and functions, however little is known about the interactive effects between these two structuring species. To this end, the abundance of both macrophytes was quantified and monitored in invaded and non-invaded localities of the South Eastern coast of Spain (Murcia Region) over an 8-year period (2007-2014). Results indicate that no differences were highlighted between invaded and no invaded meadows and all the monitored meadows showed stable or progressive trends in shoot density, meadow cover and net population growth. Regardless of the depth, in all of the invaded localities C. cylindracea biomass present inside the seagrass leaf canopy was about 10 to 50–fold lower than that measured just outside the leaf canopy. In summary, our results do not support the existence of a long-term competitive interaction between the invasive alga and the native seagrass, at least in the studied meadows and at the meadow level. C. cylindracea forms huge biomass gradients associated to the seagrass meadow edges that are stable with time, which suggests the existence of highly limiting conditions for algal growth and survival under the P. oceanica leaf canopy. Future research on such limiting factors could help us to understand the invasive capacity of the alga and the factors involved in the resistance of native habitats to bioinvasions.

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