scholarly journals Hepato-Renal-Curative Effect of the Herbal Supplement of Aloe vera Linn Gel versus Moringa oleifera on Acetaminophen-Induced Damage on the Liver and Kidney of Wistar Rats (Rattus novergicus)

2021 ◽  
pp. 12-23
Author(s):  
Chukwunyere Ogechi Abaekwume ◽  
Hope Delesi Kagbo
Keyword(s):  
Normal Control ◽  
Wistar Rats ◽  
Moringa Oleifera ◽  
Renal Toxicity ◽  
Aloe Vera ◽  
Control Treatment ◽  
Protective Effects ◽  
Herbal Supplement ◽  
Liver And Kidney ◽  
Animal House

Background: Acetaminophen (APAP, paracetamol) is the most frequently used over-the counter analgesic and antipyretic drug. Conversely, its overdose leads to both liver and kidney damage. Several scientific reports have focused majorly on protective effects of medicinal plants on APAP –induced hepato-renal-toxicity. actually, there is a dearth of work on the hepato-renal-curative effects of the herbal drugs supplements on APAP induced toxicity. Aims: In the present study, Aloe vera (ALOV) gel versus Moringa oleifera (MORN) leaf supplement effects was evaluated curatively against Acetaminophen (APAP) induced hepato- renal-toxicity.  Study Design:  This study was an experimental study in the Animal House of the Department of Pharmacology University of Port Harcourt. The work lasted for 7 days. Methodology: Twenty adult wistar rats weighing 185-220 g were divided into four (4) groups of five (5) animals each and treated orally as follows: group 1(normal control) received distilled water (7days), group 2 received 1 g/kg acetaminophen (APAP) (2 days), whereas group 3-4  received APAP (2 days) followed by 500 mg/kg of ALOV and MORN supplements respectively for 5 days. At the end of the experiment, animals from different groups were anaesthesized, the liver and kidney tissues were dissected and blood collected subjected to different biochemical, antioxidants, and histopathological test. Statistical Analysis: was done using One-Way Anova followed by Tukey’s Post-hoc Test. Results: APAP caused significant (P<0.05) decrease in creatinine with significant (P<0.01) increase and decrease in liver enzymes and renal catalase levels respectively in relation to normal control. Treatment of rats with the ALOV and MORN supplements attenuated the elevated liver and kidney biochemistry as well as improved histopathological alterations by APAP treatment. Conclusion: The supplements demonstrated restorative ability. MORN and ALOV supplements extract can be suggested as a convincing remedy against APAP-induced hepato-renal-toxicity.

scholarly journals Comparative Effect of Ginger (Zingiber officinale) Supplement on Hepato-renal Damages Induced by Acetaminophen Toxicity in Wistar Rats

2021 ◽  
pp. 1-12
Author(s):  
Chukwunyere Ogechi Abaekwume ◽  
Hope Delesi Kagbo
Keyword(s):  
Normal Control ◽  
Wistar Rats ◽  
Research Work ◽  
Kidney Damage ◽  
Control Treatment ◽  
Herbal Drugs ◽  
Acetaminophen Toxicity ◽  
Liver And Kidney ◽  
Animal House ◽  
Liver And Kidney Damage

Background: Acetaminophen toxicity is one of the widespread causes of both planned and unplanned health related issues in the world today. It is a familiar cause of liver and kidney damage particularly in adolescents and children. Aims: With the growing focus in the mitigating and therapeutic roles of herbal drugs in toxicity studies, it is perceptible to investigate the comparative, prophylactic and curative effect of the ginger supplement on acetaminophen-induced liver and kidney damage using wistar rats as model. Methodology: Study Design: Twenty (20) adult male wistar rats weighing185 g-220 g was obtained from animal house of the department of Pharmacology University of Port Harcourt were divided into four groups having five rats each.  Methodology: Twenty adult wistar rats were divided into four (4) groups of five animals each and treated orally as follows: Group A (normal control) received distilled water (7days), group B received 1000mg/kg acetaminophen (APAP) (2 days). Group C Ginger (GINE+APAP) received ginger supplements 500mg/kg respectively one hour before administration of APAP for 7 days, whereas group D received APAP (2 days) followed by 500mg/kg Ginger for 5 days (APAP+GINE). At the end of the experiment, animals from different groups were anaesthesized, blood samples collected, liver and kidney tissues dissected and subjected to different biochemical, antioxidants, and histopathological test. Statistical: Analysis was done using One Way Anova and Tukey’s Post-hoc Test.  Results: APAP caused significant (P<0.05) decrease in creatinine with significant (P<0.01) increase and decrease in liver enzymes and hepatic catalase levels respectively in relation to normal control. Treatment of rats with the ginger supplements attenuated the elevated liver and kidney biochemistry, histopathological alterations by APAP treatment. Conclusively: The extracts demonstrated therapeutic ability and the herbal drugs may be more effective when use prophylactically than curatively. Further research work is needed to validate the finding.

scholarly journals Ameliorative Effect of Nutmeg (Myristica fragrans) Supplement against Acetaminophen - Induced Hepato-Renal Toxicity in Wistar Rats

2020 ◽  
pp. 1-14
Author(s):  
Chukwunyere Ogechi Abaekwume ◽  
Hope Delesi Kagbo
Keyword(s):  
Wistar Rats ◽  
Renal Toxicity ◽  
Negative Control ◽  
Renal Diseases ◽  
Renal Tubules ◽  
Distilled Water ◽  
Ameliorative Effect ◽  
Liver And Kidney ◽  
Group B ◽  
Animal House

Background: Recently, many drugs have been developed and used for the treatment of hepatic and renal diseases. Nutmeg apart from being utilized as kitchen spices in Nigeria has also been used for healing and medicinal purposes. Aims: In the present study, nutmeg supplement was evaluated against Acetaminophen (APAP) induced hepato- renal-toxicity. Study Design: Twenty (20) adult wistar rats weighing185-220 were obtained from animal house of the department of  Pharmacology University of Port Harcourt were divided into four groups having 5 rats each (n=5). Methodology: The animals were divided into four groups of 5 rats each: Group A (normal control) were administered distilled water, group B (negative control) received a single dose of acetaminophen (1000 mg/kg) for two days. Group C received 500 mg/kg body weight of nutmeg supplement one hour before receiving 1000 mg/kg acetaminophen, while group D received acetaminophen (1000 mg/kg) only on day 1 and 2 and the drug extracts on day 3-7. All dosage was dissolved in distilled water orally. The experiment lasted for seven days. Twenty four hours after drugs administrations the animals in each group were anaesthetized. Blood samples were collected and animals sacrificed, liver and kidney tissues removed for various histopathological, biochemistry, antioxidant and haematological examinations using standard procedures. Statistical analysis was done using ANOVA and Tukey poc-hoc Test. Results: Administration of nutmeg supplement orally effectively restrained APAP-induced alterations in the activities of hepatic (48.60-38.00) and renal markers and Antioxidant enzymes in liver (0.43-0.53). The hepatic and renal architecture of APAP administered rats showed distorted liver and kidney tissues, hepatic vacuolations, destroyed glomerular and renal tubules. Nutmeg+APAP as well as APAP+ Nutmeg administrations were able to ameliorate the effects of APAP administration. Conclusion: The result indicated that APAP overdosed is hepato-renal- toxic and Nutmeg supplement possessed hepato-prote-curative properties as well as renal-prote-curative properties against APAP-induced damage in rats.

Toxicological evaluations of methanolic extract of Moringa oleifera leaves in liver and kidney of male Wistar rats

2013 ◽  
Vol 24 (4) ◽  
Author(s):  
Ademola A. Oyagbemi ◽  
Temitayo O. Omobowale ◽  
Ibraheem O. Azeez ◽  
John O. Abiola ◽  
Rahamon A.M. Adedokun ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Moringa Oleifera ◽  
Methanolic Extract ◽  
Male Wistar Rats ◽  
Liver And Kidney ◽  
Moringa Oleifera Leaves

Acute effects of methiocarb on oxidative damage and the protective effects of vitamin E and taurine in the liver and kidney of Wistar rats

2012 ◽  
Vol 29 (1) ◽  
pp. 60-71 ◽  
Author(s):  
Sibel Ozden ◽  
Betul Catalgol ◽  
Selda Gezginci-Oktayoglu ◽  
Ayse Karatug ◽  
Sehnaz Bolkent ◽  
...  
Keyword(s):  
Vitamin E ◽  
Oxidative Damage ◽  
Wistar Rats ◽  
Protective Effects ◽  
Acute Effects ◽  
Liver And Kidney

scholarly journals Protective effects of Curcuma longa rhizomes ethyl acetate extract against alcohol induced oxidative stress and nephrotoxicity in female Wistar rats

2020 ◽  
Vol 18 (1) ◽  
Author(s):  
Ofem Eteng ◽  
C.A. MOSES ◽  
J. ENOBONG ◽  
A.J. AKAMO ◽  
D.I. AKINLOYE ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Ethyl Acetate ◽  
Normal Control ◽  
Wistar Rats ◽  
Ethyl Acetate Extract ◽  
Curcuma Longa ◽  
Control Group ◽  
Protective Effects ◽  
Female Wistar Rats ◽  
Different Doses

Abstract. Eteng OE, Moses CA, Enobong J, Akamo AJ, Akinloye DI, Ugbaja RN, Akinloye OA. 2020. Protective effects of Curcuma longa rhizomes ethyl acetate extract against alcohol induced oxidative stress and nephrotoxicity in female Wistar rats. Biofarmasi J Nat Prod Biochem 21: 5-12. This study aimed to evaluate the protective effect of Curcuma longa Linn. (syn. Curcuma domestica Val.) rhizomes ethyl acetate extract (CLREAE) facing alcohol-induced oxidative stress and nephrotoxicity. Thirty female (30) Wistar rats were categorized randomly into six groups. Groups 1, 2, 3, 4, 5 and 6 were treated with normal saline; 20% ethanol; 100 mg of CLREAE + 20% ethanol; 200 mg of CLREAE + 20%; 350 mg of CLREAE + 20% ethanol and 350 mg of CLREAE respectively for 14 days. A significant (p<0.05) decrease in the SOD, CAT and GPx activities and GSH concentration of rat treated with only 20% ethanol were found when compared to the normal control group, whereas a significant (P<0.05) increase in the groups pretreated with different doses of the CLREAE were also found when compared to groups with only 20% ethanol treatment. Thus, comparing to the normal control group, treatment with the CLREAE fetched a significant (p<0.05) decrease in the renal biomarkers (creatinine and urea).  Whilst, comparing to the groups with 20% methanol treatment, a significant (p<0.05) increase happened in the groups pretreated with different doses of the CLREAE. There was a significant (p<0.05) decrease on Kidney MDA level in rats pretreated with different doses of CLREAE compared with the normal control. It was shown in the results of the histology that there was a physiologic recovery in the kidney tissues as groups were treated with different doses of the CLREAE. Evidenced by reduced necrosis of tubular and glomerular epithelial, the signs of protection against toxicity were found on the rats. The study suggested that through in vivo free radical scavenging ability, the CLREAE has protective effects against alcohol-induced oxidative stress and nephrotoxicity in female Wistar rats.

scholarly journals EFFECT OF VITAMIN E ACETATE SUPPLEMENTATION ON THYROID HORMONE-SENSITIVE ORGANS FOLLOWING EXOGENOUS L- THYROXINE TREATMENT

2018 ◽  
Vol 11 (4) ◽  
pp. 123
Author(s):  
Sabyasachi Sinha ◽  
Arijit Chakraborty ◽  
Chiranjit Mondal ◽  
Amar K Chandra
Keyword(s):  
Oxidative Stress ◽  
Body Weight ◽  
Vitamin E ◽  
Thyroid Hormone ◽  
Health Management ◽  
Control Treatment ◽  
Protective Effects ◽  
Vitamin E Acetate ◽  
Liver And Kidney ◽  
Hormone Sensitive

 Objective: L-thyroxine is used for control and prevention of many thyroidal diseases, though it may cause damages in thyroid hormone-sensitive organs, namely, liver and kidney. Reports on the protective effects of any antioxidants in L-thyroxine induced oxidative stress are scanty. Thus, L-thyroxine induced oxidative stress and its prevention by Vitamin E supplementation have been studied in the present investigation.Methods: Adult, male Wister rats were divided into four groups of six animals each, and L-thyroxine (T4) (0.3 mg/kg body weight) was administered intraperitoneally in the treated group. Similarly, L-thyroxine (T4), at the above-mentioned dose, and Vitamin E acetate (100 mg/kg of body weight/ day orally) coadministered simultaneously (T4+VE) in the next group. Third group was administered only with Vitamin E, and the remaining group kept as control. Treatment continued regularly for 15 and 30 days. Animals were sacrificed after completion of treatment. Lipid peroxidation (LPO) level, superoxide dismutase (SOD), catalase, and glutathione peroxidase (GPx) activities were assayed in liver and kidney along with their histology. Obtained results were interpreted statistically against their respective control groups.Results: Body weight was significantly decreased, and relative kidney weight was increased after L-thyroxine administration as compared to control (p<0.05). LPO level, SOD and catalase activities were significantly enhanced in L-thyroxine treated groups, whereas GPx activity was decreased. However, LPO level and the activities of those enzymes along with body weight and organ weights were almost restored their normal in L-thyroxine and Vitamin E coadministered group treated for 15 days and 30 days, respectively.Conclusion: Exogenously administered L-thyroxine causes oxidative stress in liver and kidney that in turn generates reactive oxygen species resulting cell damages. Vitamin E acetate supplementation reduces these adverse effects on liver and kidney and thus acts as a beneficial health management agent.

scholarly journals Hepatoprotective and Nephroprotective Potentials of Aqueous Leaves ExtractofLimoniaacidissimain Phenylhydrazine-Induced AnaemicWistar Rats

1970 ◽  
Vol 7 (1) ◽  
pp. 27-33
Author(s):  
Nwauche, K. T. ◽  
Anacletus, F. C ◽  
Ugwu, G. M
Keyword(s):  
Folic Acid ◽  
Normal Control ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Liver Enzymes ◽  
Alanine Transaminase ◽  
Phytochemical Analysis ◽  
Vitamine B12 ◽  
Liver And Kidney ◽  
Aqueous Leaf Extract

Bioactive composition of Limoniaacidissima leaf and liver enzyme levels and kidney status ofphenlyhydrazine (phz)-induced anaemicwistar rats were investigated. A total of forty two (42) wistar rats weighing between 150-200g were used. The animals were randomly divided into seven (7) groups of six rats each. Folic acid and vitamin B12 were used as reference drugs. Aqueousextract of Limoniaacidissima leaves was administered to the rats for four (4) weeks period.Qualitative phytochemical analysis on the dry leaf samples revealed the presence of the following in low abundance: alkaloid, saponins, cardiac glycoside, steroids, phenols, terpenoids and proteins while flavonoids were in high abundance. A significant increase (p<0.05) was observed in the activities of serum alanine transaminase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) in thephenylhtdrazine-inducedanaemic non- treated groups in comparison to normal control. There was a significant increase (p<0.05) in urea, creatinine, potassium and sodium levels in the phenylhydrazine-induced anemic non- treated rats compared to the normal control. The other groups treated with aqueous leaf extract of Limoniaacidissimasubsequently ameliorated these effects on the liver and kiney markers. Vitamine B12 and folic acid also reversed these effects on the liver enzymes and kiney markers.This outcome illustrates that the aqueous extract of the leaf of Limoniaacidissimapossess a protective potentials on the liver and kidney of phenylhydrazine- induced wistar rats.

scholarly journals Haematology and Serum Biochemical Indices of Lambs Supplemented with Moringa oleifera, Jatropha curcas and Aloe vera Leaf Extract as Anti-Methanogenic Additives

Antibiotics ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 601
Author(s):  
Abiodun Mayowa Akanmu ◽  
Abubeker Hassen ◽  
Festus Adeyemi Adejoro
Keyword(s):  
Alkaline Phosphatase ◽  
Plant Extracts ◽  
Biochemical Parameters ◽  
Dry Matter ◽  
Moringa Oleifera ◽  
Aloe Vera ◽  
Control Treatment ◽  
Serum Biochemical Parameters ◽  
Enteric Methane ◽  
Serum Biochemical

Medicinal plants have been found to be effective in a wide range of applications in ruminant animals. However, some plant extracts may be toxic to animals, depending on their seconday metabolite composition and dose, and therefore, animal trials are needed to validate their safety when used as anti-methanogenic additives. This study investigated the effect of three plant extracts used as anti-methanogenic dietary additives, on the haematology and serum biochemical parameters in sheep. Methanolic extracts of Moringa oleifera (MO), Jatropha curcas (JC) and Aloe vera (AV) were orally dosed as experimental treatments for 75 days to sheep, and their effect on the haematology and serum biochemical parameters of SA Mutton Merino (SAMM) lambs were compared with sheep on a control treatment without any additive treatment. Extracts of MO, JC and AV were extracted in 100% methanol, freeze-dried, and reconstituted in distilled water. A total of 40 lambs were ranked according to their body weight into a group of four and one sheep at a time was randomly allocated into four dietary treatments which include a control treatment, and treatment with either MO, JC or AV extract. Lambs were drenched twice daily with doses equivalent to 50 mg/kg dry matter intake (DMI) based on previous week feed consumption. Blood samples were collected via jugular vein puncture and analysed for haematology and serum biochemistry parameters, using standard procedures. The results of the haematological analysis showed that most haematological parameters were not affected by plant extract used as anti-methanogenic additives (p > 0.05), except for higher white blood cell (WBC) and lymphocytes counts recorded in control lambs and lambs in the AV treatment. All serum biochemical properties (except alkaline phosphatase) were not different (p > 0.05) between the control and lambs treated with plant extracts. Alkaline phosphatase was influenced by the plant extract (p < 0.05), with lambs receiving MO, JC and AV having lower alkaline phosphatase concentrations compared to lambs on the control diet without any additive. The result of the study showed that extracts of MO, JC and AV were not toxic to sheep when used as antimethanogenic additives at the recommended dose of 50 mg/kg dry matter feed which had proved previously to be effective in reducing enteric methane emission. Therefore, these plant extracts could be used safely as alternative dietary additives to reduce enteric methane emission and boost the productivity of SA Mutton Merino sheep.

scholarly journals Protective effects of aloe vera gel (aloe baberdensis Miller) on aluminum chloride-induced reproductive toxicity in male Wistar rats

2021 ◽  
Vol 25 (2) ◽  
Author(s):  
Busuyi Kolade Akinola ◽  
Toluwase Solomon Olawuyi ◽  
Lekia Damilola Daniel ◽  
Victor Okoliko Ukwenya ◽  
Bolanle Christiana Faleye
Keyword(s):  
Aluminum Chloride ◽  
Wistar Rats ◽  
Reproductive Toxicity ◽  
Aloe Vera ◽  
Protective Effects ◽  
Aloe Vera Gel ◽  
Male Wistar Rats

scholarly journals EVALUATION OF TOXICITY PROFILES OF DIFFERENT BRANDS OF PIROXICAM IN WISTAR RATS

2021 ◽  
Vol 9 (6) ◽  
pp. 36-44
Author(s):  
B. Cletus Lemii ◽  
A. Georgewill Onwunari
Keyword(s):  
Wistar Rats ◽  
Liver Toxicity ◽  
Male Rats ◽  
Control Group ◽  
Treatment Groups ◽  
Clinical Dose ◽  
Port Harcourt ◽  
Liver And Kidney ◽  
Animal House ◽  
Department Faculty

BACKGROUD: , a non-steroidal anti-inflammatory drug (NSAID), has been identified to be one of such drugs with great potentials for abuse, addiction and death as well as complaints on the significant dissimilarities and variations of possible organ toxicities across different brands. This study aimed at evaluating toxicity profiles of different brands of in male rats. Healthy thirty (30) male rats, weighing between 180 and 230grams were procured from the animal house of the Pharmacology department, Faculty of Basic Clinical Sciences in University of Port Harcourt, Nigeria. METHODS: The animals were divided into six (6) groups of five (5) animals each. Group1-control group while group2 to 6 were treatment groups. Thereafter, the clinical dose (0.285mg/kg) of brands (A Brand, B Brand, C Brand, D Brand and E Brand) were administered on treatment groups. The analysis was done at the end of the experiment. RESULT: Reveals that, four out of the fives brands demonstrated significant (p&lt;0.05) toxicities on liver and kidney. Two of the brands has predilection for liver toxicity while another two exhibited kidney toxicities. The heart organ was spared from toxic effects of all the five brand understudied. CONCLUSION: The degree of organ toxicities with different brands of varies, however, certain brands tend to have toxicity predilection for either the liver or kidney.

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