scholarly journals Ameliorating Effects of Omega-3 Fatty Acids on Underlying Mechanisms of Type 2 Diabetes

2020 ◽  
pp. 1-7
Author(s):  
Abroo Fatima Qazi ◽  
Din Muhammad Shaikh
Keyword(s):  
Fatty Acids ◽  
Wistar Rats ◽  
Positive Control ◽  
Control Group ◽  
P Value ◽  
Omega 3 Fatty Acids ◽  
Omega 3 ◽  
Group A ◽  
Group B

Aims: The current study investigated the effects of polyunsaturated Omega-3-fatty acids on underlying mechanism linked with diabetes in streptozocin induced type 2 diabetic Wistar rats. Study Design:  Experimental analytical Study. Place and Duration of Study: The study was conducted at Isra University Hyderabad and Sindh Agricultural University, Tandojam between November 2016 and November 2018. Methodology: Seventy-five Wistar rats were assorted to five groups (15 rats per group): negative control group A and positive control group B and experimental groups C, D and E. Rats within group B,C,D, and E were injected with streptozocin (65 mg/kg body weight) to induce diabetes. Experimental groups C, D and E received Omega-3-fatty acid supplemented food in 0.3 g, 0.4 g and 0.5 g/kg bodyweight dosage for 12 weeks, respectively. Results: Omega-3-fatty acids treated rats showed significant decrease in blood glucose level and rise in serum insulin as compared to positive control group (p-value = 0.001). At the same time, they showed significantly increased expression of insulin gene along with transcription factors: PDX1 and NKX6.1 as compared to group A (p-value = 0.001). Conclusion: It is concluded that O3FAs reduces insulin resistance in Streptozocin-induced diabetic Wistar rats by modulating the transcription factors essential for insulin gene expression.

scholarly journals “A Comparative Study of Efficacy of Atorvastatin Alone and Atorvastatin with Omega-3 Fatty Acids Combination in Patients with Hyperlipidaemia Attending Tertiary Care Hospital”

2021 ◽  
pp. 482-490
Author(s):  
C. Srinivasa ◽  
K. La Kshminarayan ◽  
V. Srinivas ◽  
B. V. S. Chandrasekhar
Keyword(s):  
Fatty Acids ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Hdl Cholesterol ◽  
Low Density ◽  
Care Hospital ◽  
Omega 3 Fatty Acids ◽  
Omega 3 ◽  
Group A ◽  
Group B

Background: Current treatment with statins has become an integral part of vascular diseases but monotherapy has a significant residual event rate. Due to particularly one of the factor associated with atherogenic lipid phenotype that is characterized by a low high-density lipoprotein (HDL) cholesterol and increase in non-HDL cholesterol like Low-Density Lipoprotein (LDL). Omega-3 Fatty acids have demonstrated a preventiverole in primary and, particularly secondary cardiovascular diseases.  Hence this study was planned to compare the efficacy of Atorvastatin alone with Atorvastatin and Omega-3 fatty acids in treatment in hyperlipidaemia patients. Methods: The study was comparative, randomized, and prospective and open labeled conducted in MI patients. A total of 100 patients were selected based on inclusion and exclusion criteria. They were divided randomly into two Groups (Group–A and Group-B). Group-A was given Atorvastatin 10mg/day and Group-B was given Atorvastatin 10mg/day and Omega-3 fatty acids 600mg/day for 6 months. Follow up was done every month and efficacy was measured by assessing the lipoprotein levels in serum. Results: The results were compared before treatment and after 6 months treatment.The levels were significantly decreased Total Cholesterol (TC), LDL, Low-Density Lipoprotein (VLDL), Triglycerides (TG) and HDL levels were increased in Group–A and Group-B. When these results compared between two Groups the HDL levels were increased also it shown high significance (<0.001) but there were no significance changes in other cholesterol levels. Conclusion: The present study results showed that Atorvastatin and Omega-3 fatty acids treatment was more effective than Atorvastatin alone treatment in improving HDL-C levels from base line and it may have a additive effect in major coronary artery diseases.

scholarly journals Oral supplementation with omega-3 fatty acids and inflammation markers in patients with chronic kidney disease in hemodialysis

2020 ◽  
Vol 45 (8) ◽  
pp. 805-811
Author(s):  
José A. Valle Flores ◽  
Juan E. Fariño Cortéz ◽  
Gabriel A. Mayner Tresol ◽  
Juan Perozo Romero ◽  
Miquel Blasco Carlos ◽  
...  
Keyword(s):  
Chronic Kidney Disease ◽  
Fatty Acids ◽  
Kidney Disease ◽  
Omega 3 Fatty Acids ◽  
Omega 3 ◽  
Inflammation Markers ◽  
Oral Supplementation ◽  
Reactive Protein ◽  
Group A ◽  
Group B

Chronic kidney disease (CKD) is an increasingly common public health problem that increases the risk of death because of cardiovascular complications by 2–3 times compared with the general population. This research concerns a prospective, randomized, double-blind study in patients with CKD undergoing hemodialysis. The participants were assigned to 1 of 2 groups: the study group (group A; 46 patients) received 4 capsules (2.4 g) of omega-3 fatty acids daily during the 12-week intervention, while patients in the control group (group B; 47 patients) received 4 capsules of paraffin oil. The patients’ general characteristics, nutritional indicators, renal disease markers and inflammatory markers (C-reactive protein, interleukin (IL)-6, IL-10, and tumour necrosis factor alpha (TNF-α)) were evaluated. No differences were found between the general characteristics of the patients (P < 0.05), and no differences were shown in the nutritional indicators and markers of kidney disease (P < 0.05). Patients in group A showed significant decreases in levels of C-reactive protein, IL-6, TNF-α, and the IL-10/IL-6 ratio after 12 weeks of supplementation (P < 0.05). Patients in group B did not show any significant changes in concentrations of inflammatory markers during the intervention (P < 0.05). In conclusion, oral supplementation with omega-3 fatty acids produces a significant decrease in the concentrations of inflammation markers in patients with chronic kidney disease on hemodialysis. Novelty Oral supplementation with omega-3 fatty acids produced significant decreases in the concentrations of inflammation markers. This supplementation could be given to patients with uremic syndrome and coronary heart disease to reduce cardiovascular risk.

scholarly journals Decrease of Atherosclerosis Markers and Oxidative Stress After 1 Year Administration of Omega-3 Fatty Acids Supplements in Patients with Metabolic Syndrome

2014 ◽  
Vol 21 (3) ◽  
pp. 175-183
Author(s):  
Andreea Dragomir ◽  
Emila Rusu ◽  
Mihaela Posea ◽  
Gabriela Radulian
Keyword(s):  
Oxidative Stress ◽  
Metabolic Syndrome ◽  
Fatty Acids ◽  
Omega 3 Fatty Acids ◽  
Omega 3 ◽  
Atherosclerosis Progression ◽  
Group A ◽  
Group B ◽  
The Impact ◽  
And Oxidative Stress

AbstractBackground and Aims: To assess the impact of 1 year administration of omega-3 fatty acids supplements on oxidative stress parameters and atherosclerosis progression. Material and Methods: A total of 284 patients with metabolic syndrome, aged 61±6.7 years, without clinical evidence of atherosclerosis were allocated to 2 groups, matched by sex and age: group A (140 patients) - diet according to ESC/EASD recommendations; group B (144 patients) - the same diet + capsules of fish oil (1g eicosapentanoic acid, 1g docosahexanoic acid, 0,1g a-tocopherol acetate). Body fat (BF) was measured by bioimpedance analysis. For oxidative stress evaluation we used the FormOx systems monitor on a blood drop and for progression of atherosclerosis the intima-media thickness (IMT) at common carotid artery. Patients were evaluated at baseline, after 6 months and 1 year. Results: IMT significantly decreased in group B vs group A at 1 year (p<0.0001) and was correlated with %BF (p<0.001), waist-to-hip ratio (WHR) (p=0.002), leptin (p< 0.001), adiponectin (p<0.05), leptin/adiponectin ratio (p< 0.001) and oxidative stress (p<0.001). Conclusions: One year administrations of omega-3 PUFA enriched diet reduces cardiovascular risk of metabolic syndrome patients, resulting in a significant decrease of oxidative stress and atherosclerosis progression.

scholarly journals Omega-3 fatty acids reduce the development of preneoplasic lesions

2009 ◽  
Vol 22 (2) ◽  
pp. 237-244 ◽  
Author(s):  
Viviana Teixeira Henriques ◽  
Cristina Maria Ganns Chaves Dias ◽  
Sylvia do Carmo Castro Franceschini ◽  
Céphora Maria Sabarense ◽  
Neuza Maria Brunoro Costa ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Wistar Rats ◽  
Treated Group ◽  
Aberrant Crypt Foci ◽  
Colorectal Carcinogenesis ◽  
Control Group ◽  
Omega 3 Fatty Acids ◽  
Omega 3 ◽  
Preneoplastic Lesions ◽  
Male Wistar Rats

OBJECTIVE: The purpose of this study was to evaluate the anticancer potential of dietary omega-3 supplementation to reduce induced intestinal preneoplastic lesions in Wistar rats. METHODS: A total of 58 11-week-old male Wistar rats (Rattus norvergicus, albinus variety, Rodentia) were distributed into two groups: a control group (n=25) and an omega-3-treated group (n=28). Aberrant crypt foci were induced by 1,2-dimethylhydrazine. Tissue incorporation of the supplemented omega-3 fatty acids was evaluated by determining the fatty acid profiles of intra-abdominal fat and the liver with gas chromatography. RESULTS: The omega-3 group presented lower weight and lower food intake (p<0.05) than the control group. The number of aberrant crypt foci decreased 55.34% in response to omega-3 supplementation. Foci with more than three crypts decreased 57.14% between weeks 13 and 28. There was no statistical difference for the docosahexaenoic acid content in the liver of the omega-3 group between week 6 and weeks 13 and 28. CONCLUSION: These results suggest that omega-3 may slow the progress of colorectal carcinogenesis.

scholarly journals EFEK EKSTRAK DAUN SIRSAK (ANNONA MURICATA L.) PADA KADAR KOLESTEROL TOTAL TIKUS WISTAR

2013 ◽  
Vol 4 (1) ◽  
Author(s):  
Ira Posangi ◽  
Jimmy Posangi ◽  
Jane Wuisan
Keyword(s):  
Total Cholesterol ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Positive Control ◽  
Modern Medicine ◽  
Control Group ◽  
Annona Muricata ◽  
Cholesterol Levels ◽  
Group A ◽  
Group B

Abstract: Treatment using medicinal plants has already been applied before modern medicine. Soursop (Annona muricata L.) leaves have been used for the treatment of various diseases. The purpose of this study was to test whether the soursop leaf extract could lower the total cholesterol levels of wistar rats used as test animals. Prior to lowering the cholesterol levels, they first had to be raised, and to raise the level of total cholesterol, all test animals were induced by propyl thiouracyl (PTU). Rosuvastatin was used for positive control group F. Wistar rats were divided into six groups. Group A was the normal control group. Group B was given just PTU. Groups C, D, and E, after having been given PTU, were given respective doses of 50mg, 100mg, and 200mg/day of soursop leaf extract. Group F was given PTU and then rosuvastatin. The results showed that the total cholesterol levels in rats given soursop leaf extract or rosuvastatin were lower than rats that were not given soursop leaf extract or rosuvastatin. Conclusion: soursop leaf extract could lower the total cholesterol levels of wistar rats. Key words: soursop leaves, cholesterol Abstrak: Pengobatan menggunakan tanaman berkhasiat obat sudah lebih dulu diterapkan dari obat-obat modern. Daun sirsak telah digunakan untuk pengobatan bermacam penyakit. Tujuan penelitian ini untuk menguji apakah ekstrak daun sirsak dapat menurunkan kadar total kolesterol tikus wistar yang digunakan sebagai hewan uji. Untuk menaikkan kadar total kolesterol hewan uji diinduksi dengan PTU. Untuk kontrol positif digunakan rosuvastatin. Tikus wistar dibagi dalam 6 kelompok yang terdiri Kelompok A sebagai kontrol normal, Kelompok B diberikan PTU, Kelompok C, D, dan E diberikan PTU kemudian diberikan berturut-turut ekstrak daun sirsak dosis 50mg, 100mg, dan 200mg/hari, Kelompok F diberikan PTU kemudian rosuvastatin. Hasil penelitian menunjukkan kadar kolesterol total pada tikus yang diberikan ekstrak daun sirsak atau rosuvastatin lebih rendah daripada tikus yang tidak diberikan ekstrak daun sirsak atau rosuvastatin. Simpulan: ekstrak daun sirsak dapat menurunkan kadar total kolesterol tikus wistar.Kata kunci: daun sirsak, kolesterol

Silymarin and dimercaptosuccinic acid ameliorate lead-induced nephrotoxicity and genotoxicity in rats

2015 ◽  
Vol 35 (4) ◽  
pp. 398-403 ◽  
Author(s):  
Y Alcaraz-Contreras ◽  
RP Mendoza-Lozano ◽  
ER Martínez-Alcaraz ◽  
M Martínez-Alfaro ◽  
MA Gallegos-Corona ◽  
...  
Keyword(s):  
Lead Acetate ◽  
Chelating Agent ◽  
Blood Lead ◽  
Positive Control ◽  
Dimercaptosuccinic Acid ◽  
Control Group ◽  
Lead Levels ◽  
Group A ◽  
Group B ◽  
Group D

We studied the effect of silymarin and dimercaptosuccinic acid (DMSA), a chelating agent that was administered individually or in combination against lead (Pb) toxicity in rats. Wistar rats (200 ± 20) were randomly divided into five groups. Group A served as a control. Groups B–E were exposed to 2000 ppm of lead acetate in drinking water for 8 weeks. Group B served as a positive control. Group C received silymarin (100 mg kg−1 orally) for 8 weeks. Group D received DMSA (75 mg kg−1 orally) once daily for the last 5 days of treatment. Group E received DMSA and silymarin as groups C and D, respectively. The effect of Pb was evaluated and accordingly the treatments on blood lead levels (BLLs), renal system, and genotoxic effects were calculated using comet assay. The BLLs were significantly increased following the exposition of lead acetate. The administration of silymarin and DMSA provided reduction in BLLs. Silymarin and DMSA provided significant protection on the genotoxic effect of Pb. The toxic effect of Pb on kidneys was also studied. Our data suggest that silymarin and DMSA improve the renal histopathological lesions.

scholarly journals Effect of acupuncture TENS versus conventional TENS on post cesarean section incision pain

2018 ◽  
Vol 7 (9) ◽  
pp. 3738
Author(s):  
Priti A. Mehendale ◽  
Mayur T. Revadkar
Keyword(s):  
Pain Intensity ◽  
Rating Scale ◽  
Control Group ◽  
P Value ◽  
Random Allocation ◽  
Lower Segment Caesarean Section ◽  
Post Operative Pain ◽  
Group A ◽  
Group B ◽  
Pain Rating Scale

Background: There is evidence regarding beneficial use of Transcutaneous Electrical Nerve Stimulation (TENS) on post Lower Segment Caesarean Section (LSCS) incision pain. However, efficacy of different types of TENS following C section pain has not yet been explored adequately.Methods: 96 women who had recently undergone LSCS were included for the study. The subjects were in the age group of 20 to 40 years (25.84±3.96); having pain intensity 4 or more on Numerical Pain Rating Scale (NPRS). They were divided into three groups by random allocation method; namely Group A: Acupuncture (Low/Motor) TENS, Group B: Conventional (High/Sensory) TENS and Group C: Control group. Group A and B received specific type of TENS twice a day for 15 minutes. Control group C did not receive any TENS intervention. All subjects received standard post-operative medications and physiotherapy. Pain intensity was recorded on NPRS pre and post intervention.Results: Both Acupuncture TENS and Conventional TENS significantly decreased post-operative pain intensity as compared to control group (p value <0.0001).Conclusions: Both, acupuncture and conventional TENS are equally effective in reducing post LSCS incision pain at a strong and non-painful intensity.

scholarly journals Effects of aqueous leaf extract of Corchorus olitorius on the testis and blood testosterone level of adult Wistar rats

2021 ◽  
Vol 10 (2) ◽  
pp. 024-030
Author(s):  
Godwin Chinedu Uloneme ◽  
Demian Nnabuihe Ezejindu ◽  
Darlington Cyprian Akukwu ◽  
Amadi Chibundu Chiekezie
Keyword(s):  
Body Weight ◽  
Wistar Rats ◽  
Day Treatment ◽  
Control Group ◽  
Corchorus Olitorius ◽  
Group A ◽  
Normal Rat ◽  
Group B ◽  
Calcium Iron ◽  
Group D

Background: The extract of Corchorus olitorius has a reasonable content of vitamins A and C, calcium, iron and fibre, and therefore enjoys a universal application in the treatment of some disease conditions, even as the whole leaf is a very important component of food in so many cultures. Purpose: The study was designed to investigate the effect of Corchorus olitorius extracts on the testis of adult Wistar rats. Method: A total number of thirty two adult Wistar rats weighing between 180 and 200 grammes separated into four groups labeled A,B,C and D respectively were used for the study. Animals in group A which served as the control group were fed with the normal rat chow and water only. The group B rats were administered 100mg/kg body weight of aqueous extract of Corchorus olitorus; while those in group C were administered 500mg/kg body weight of the extract. The group D rats received 1000mg/kg body weight of the extract. For a period of four weeks, the different experimental animal groups received the respective aforementioned treatments once daily, around nine- o’clock in the morning through oral intubation. At the end of the 28 day treatment, the animals were sacrificed and the testes harvested for histological, investigation, and through cardiac puncture, blood samples for some hormonal studies was also collected and investigated using standard laboratory standards. Results: Observations made showed that the extract produced no histological distortions, degenerative or defective effects on the testicular tissues. The testosterone levels of group B, C, and D rats were observed to be significantly higher (P<0.005) than that of the group A (control group).ound: The extract of Corchorus olitorius has a reasonable content of vitamins A and C, calcium, iron and fibre, and therefore enjoys a universal application in the treatment of some disease conditions, even as the whole leaf is a very important component of food in so many cultures. Purpose: The study was designed to investigate the effect of Corchorus olitorius extracts on the testis of adult Wistar rats. Method: A total number of thirty two adult Wistar rats weighing between 180 and 200 grammes separated into four groups labeled A,B,C and D respectively were used for the study. Animals in group A which served as the control group were fed with the normal rat chow and water only. The group B rats were administered 100mg/kg body weight of aqueous extract of Corchorus olitorus; while those in group C were administered 500mg/kg body weight of the extract. The group D rats received 1000mg/kg body weight of the extract. For a period of four weeks, the different experimental animal groups received the respective aforementioned treatments once daily, around nine- o’clock in the morning through oral intubation. At the end of the 28 day treatment, the animals were sacrificed and the testes harvested for histological, investigation, and through cardiac puncture, blood samples for some hormonal studies was also collected and investigated using standard laboratory standards. Results: Observations made showed that the extract produced no histological distortions, degenerative or defective effects on the testicular tissues. The testosterone levels of group B, C, and D rats were observed to be significantly higher (P<0.005) than that of the group A (control group).

scholarly journals Effects of Aqueous Extract of Basella alba L. leaves on Blood Cell Count in Rats

2019 ◽  
Vol 22 (1) ◽  
pp. 73-78
Author(s):  
Farhana Sabrin ◽  
AF Mohammed Shafiqul Alam ◽  
Muhammad Rashedul Islam ◽  
Md Elias Al Mamun ◽  
Jakir Ahmed Chowdhury
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Aqueous Extract ◽  
Control Group ◽  
P Value ◽  
Blood Cell Count ◽  
Basella Alba ◽  
Group A ◽  
Group B ◽  
Group D

The effect of aqueous extract of Basella alba (puishak) leaves on blood cell count of rats was studied. Forty rats of both sexes weighing between 100-150 gm were used. The rats were divided into four groups (7 rats in each group), with group A as the control group and experimental groups were denoted as B, C and D. With all aseptic precautions, aqueous extract of Basella alba (Puishak) leaves was administered into the three different doses (For group B: 60 mg/kg bw, group C: 80 mg/kg bw and group D: 100 mg/kg bw). Control group A also received distilled water as a placebo at the dose of 10 mg/kg bw for 14 days. At day 15, blood samples were collected and sent for haematological analysis. Data analysis of blood count profile of 28 rats revealed that there is an increased number of RBC, WBC and platelet count in experimental groups than in control group. ANOVA test revealed that increased blood cell counts following administration of aqueous leaves extract of keeves of B. alba were statistically significant (p value for each case was .0001<.05) than control group. Paired samples t test was performed to compare between baseline hematological parameters and parameters after 14 days of intervention. Then comparison between Group A & Group B, between Group A & Group C and between Group A & Group D were done. In all cases, probability (p) value < 0.05 was considered as statistically significant.This it is clearly evident that aqueous extract of B.alba has positive stimulant effect on blood cells count of rats. Moreover, it was found that increment of doses also increases the cell count that is positively correlated with the hypothesis. Bangladesh Pharmaceutical Journal 22(1): 73-78, 2019

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