54 article(s) in International Journal of Advancement in Life Sciences Research

Reemergence Of Chloramphenicol Sensitivity Among Salmonella Enterica Serovars Typhi And Paratyphi : A Six Year Experience In A Tertiary Care Hospital In Nepal

emerging drug resistance among salmonella typhi and paratyphi has become challenging in the treatment of enteric fever. the objective of this study was to determine the antibiotic susceptibility pattern of salmonella serotypes isolated from patients with enteric fever admitted to manipal teaching hospital, pokhara,nepal. a total of 30 salmonella enterica serovar typhi, paratyphi a, and paratyphi b isolated from cases of typhoid and paratyphoid fever admitted to manipal teaching hospital over a period from january 2012 to march 2018 were investigated. all strains were identified by standard microbiological methods and tested for in vitro antibiotic susceptibility testing,using kirby-bauer disc diffusion method following the criteria designed by the clinical and laboratory standards institute (clsi 2013). s typhi was the most predominant amongst all the isolates (18 of 30 i.e. 60%), followed by s paratyphi a (33.3%, 10 out of 30) and s paratyphi b (6.6%; 2 out of 30). overall, 91.3% of the isolates were susceptible to chloramphenicol. the percentage sensitivities towards ceftriaxone, ciprofloxacin, cotrimoxazole and ampicillin were 82.6%, 75.8%, 63% and 37% respectively. all the isolates were sensitive to imipenem and amikacin. these findings suggested that there were changing patterns of antibiotic resistance in enteric fever with reemergence of chloramphenicol sensitive salmonellae. this necessitates continuous surveillance of cases and re-evaluation of chloramphenicol therapy in salmonella infections in nepal Show More ... ... Show Less

  • Enteric Fever
  • Teaching Hospital
  • Salmonella Enterica
  • Antibiotic Susceptibility
  • Paratyphi B

food safety includes all health aspects of the food, especially microbiological quality. butter, particularly commercial butter is one of the highly consumed foods in malaysia. the research objective was to investigate the microbiological, physicochemical characteristics and structure of fatty acids of a commercial cows' butter (ccb) sold in malaysia. chemical, microbial quality and fatty acid compositions of 7 randomly purchased butter samples from the market were analyzed. the results show the existence of substantial variances in physicochemical parameters between butter samples. the range of ph was between 3.32 to 4.90. the moisture content of all commercial cows' butter samples except sample 5 and 6 was in international standard range. peroxide and iodine values of all samples were in range of international standard limit. the determination of fatty acids composition by gas chromatography showed the prevalence of the saturated fatty acids dominated by palmitic acid, with a low rate of unsaturated fatty acids, dominated by oleic acid. the results also show the presence minimum of total aerobic mesophilic bacteria (tbc) as well as psychrotrophic bacteria, while coliforms bacteria were not detected. moulds and yeasts were detected in all samples as minimum counts except samples 2 and 4 which were free from them. therefore, it was found that the microbiological quality of commercial butter samples is generally good and thus health safety Show More ... ... Show Less

  • Fatty Acids
  • Microbiological Quality
  • Microbial Quality
  • International Standard
  • Low Rate
In –Vitro Antioxidant Activity Of Methanolic Extract Of The Roots Of Bergenia Ciliata

bergenia ciliata is an essential medicinal plant used in regions where western medicines are inaccessible due to their unavailability and high cost. the methanolic extract of bergenia ciliataroots was separated for phytochemical elements and in-vitro antioxidant activity. the plant extract showed the rich outgrowth of secondary metabolites that play the role for biological activities. the higher antioxidant functioning of the plant is due to the occurrence of reactive elements like phenols and flavonoids. the antioxidant functioning of the plant extract was measured by dpph free radical scavenging assay. in dpph free radical scavenging assay the ic50 value of bergenia ciliata was found to be 11.21μg/ml, while the ic50 value of standard ascorbic acid was found to be 45.93μg/ml Show More ... ... Show Less

  • Free Radical
  • In Vitro Antioxidant Activity
  • Radical Scavenging Assay
Progress Towards And Challenges In Biological Big Data

biological big data represents a vast amount of data in bioinformatics and this could lead to the transformation of the research pattern into large scale. in medical research, a large amount of data can be generated from tools including genomic sequencing machines. the availability of advanced tools and modern technology has become the main reason for the expansion of biological data in a huge amount. such immense data should be utilized in an efficient manner in order to distribute this valuable information. besides that, storing and dealing with those big data has become a great challenge as the data generation are tremendously increasing over years. as well, the blast of data in healthcare systems and biomedical research appeal for an immediate solution as health care requires a compact integration of biomedical data. thus, researchers should make use of this available big data for analysis rather than keep creating new data as they could provide meaningful information with the use of current advanced bioinformatics tools Show More ... ... Show Less

  • Big Data
  • Health Care
  • Biomedical Research
  • Research Pattern
A Taxonomic Account Of Hover Flies (Insecta: Diptera: Syrphidae) With 4 New Records From Cold Dry Zones Of Himachal Pradesh, India

eighteen (18) species of hover flies (insecta:diptera:syrphidae) under 14 genera and 2 subfamilies has been reported from the cold dry zone of himachal pradesh which includes kinnaur, lahul & spiti and pangi range of chamba districs. 4 species namely chrysotoxum violaceum brunetti, 1923, sphiximorpha triangulifera (brunetti, 1913),mallota (mallota) orientalis (wiedemann, 1824), mallota (mallota) varicolor(walker, 1856) has been reported from the first time from this cold and dry zone zone as well as from the state of himachal pradesh. their taxonomic keys and detail diagnosis of the reported species has been discussed along with the distributional pattern of these species along the cold dry zone of himachal pradesh Show More ... ... Show Less

  • Himachal Pradesh
  • Dry Zone
  • Hover Flies
  • New Records
  • Distributional Pattern