Inhibition of prolactin promotes secondary skin follicle activation in cashmere goats

2021 ◽  
Author(s):  
Lechao Zhang ◽  
Chunhui Duan ◽  
Yunxia Guo ◽  
Yingjie Zhang ◽  
Yueqin Liu
Keyword(s):  
Gene Expression ◽  
Growth Rate ◽  
Membrane Receptor ◽  
Control Group ◽  
Follicle Development ◽  
Serum Concentrations ◽  
Anagen Phase ◽  
Cashmere Goats ◽  
Effect Of Treatment ◽  
Hair Follicle Development

Abstract The aim of this study was to investigate the involvement of prolactin (PRL) on development of secondary skin follicles in cashmere goats. Goats were randomly assigned to either a bromocriptine treatment or control group. Samples of cashmere fiber, blood and skin were collected from all goats after 1 month. The results indicated that the length, growth rate and diameter of fibers were not influenced (P > 0.05) by the inhibition of prolactin resulting from the treatment with bromocriptine. There was a tendency for increases in total follicle number, primary and secondary follicle numbers and in the ratio of secondary to primary follicles following treatment with bromocriptine, but these differences were not significant (P > 0.05). The percentage of active secondary follicles in anagen was increased (P < 0.05) in the bromocriptine-treated goats but there was no effect of treatment on the percentage of active primary follicles. Bromocriptine decreased (P < 0.05) circulating concentrations of PRL and Insulin-like growth factor 1 (IGF1) and increased (P < 0.05) those of melatonin (MT), but there was no effect of this treatment on the serum concentrations of cortisol (COR), growth hormone (GH), tetraiodothyronine (T4) and triiodothyronine (T3). In bromocriptine-treated goats mRNA expressions of PRL and MT membrane receptor 1a (MTNR1a) were decreased (P < 0.05) and mRNA expression of MT nuclear receptor (RORα) was increased (P < 0.05), but there was no effect of the treatment on expression of long PRL receptor (LPRLR), short PRL receptor (SPRLR), MT membrane receptor 1b (MTNR1b) and IGF1. It is concluded that inhibition of PRL promotes secondary hair follicle development in the anagen phase, possibly by down-regulating MTNR1a and up-regulating RORα gene expression in the skin.

scholarly journals Using WGCNA (weighted gene co-expression network analysis) to identify the hub genes of skin hair follicle development in fetus stage of Inner Mongolia cashmere goat

PLoS ONE ◽  
2020 ◽  
Vol 15 (12) ◽  
pp. e0243507
Author(s):  
Zhihong Wu ◽  
Erhan Hai ◽  
Zhengyang Di ◽  
Rong Ma ◽  
Fangzheng Shang ◽  
...  
Keyword(s):  
Network Analysis ◽  
Hair Follicle ◽  
Quantitative Polymerase Chain Reaction ◽  
Correlation Coefficients ◽  
Hair Follicles ◽  
Functional Enrichment ◽  
Follicle Development ◽  
Hub Genes ◽  
Cashmere Goats ◽  
Hair Follicle Development

Objective Mature hair follicles represent an important stage of hair follicle development, which determines the stability of hair follicle structure and its ability to enter the hair cycle. Here, we used weighted gene co-expression network analysis (WGCNA) to identify hub genes of mature skin and hair follicles in Inner Mongolian cashmere goats. Methods We used transcriptome sequencing data for the skin of Inner Mongolian cashmere goats from fetal days 45–135 days, and divided the co expressed genes into different modules by WGCNA. Characteristic values were used to screen out modules that were highly expressed in mature skin follicles. Module hub genes were then selected based on the correlation coefficients between the gene and module eigenvalue, gene connectivity, and Gene Ontology (GO)/Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. The results were confirmed by quantitative polymerase chain reaction (qPCR). Results Ten modules were successfully defined, of which one, with a total of 3166 genes, was selected as a specific module through sample and gene expression pattern analyses. A total of 584 candidate hub genes in the module were screened by the correlation coefficients between the genes and module eigenvalue and gene connectivity. Finally, GO/KEGG functional enrichment analyses detected WNT10A as a key gene in the development and maturation of skin hair follicles in fetal Inner Mongolian cashmere goats. qPCR showed that the expression trends of 13 genes from seven fetal skin samples were consistent with the sequencing results, indicating that the sequencing results were reliable.n

scholarly journals Addition of Bamboo Charcoal to Selenium (Se)-Rich Feed Improves Growth and Antioxidant Capacity of Blunt Snout Bream (Megalobrama amblycephala)

Animals ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 2585
Author(s):  
Fang Jiang ◽  
Yan Lin ◽  
Linghong Miao ◽  
Jingyuan Hao
Keyword(s):  
Gene Expression ◽  
Growth Rate ◽  
Antioxidant Enzyme ◽  
Inflammatory Cytokines ◽  
Enzyme Activities ◽  
Megalobrama Amblycephala ◽  
Control Group ◽  
Antioxidant Enzyme Activities ◽  
Bamboo Charcoal ◽  
Blunt Snout Bream

The ability of bamboo charcoal to reduce the negative effects of high dietary selenium (Se) concentrations was assessed by feeding juvenile blunt snout bream (Megalobrama amblycephala) one of five Se-rich diets (1.5 mg/kg Se; 36% protein, 8.7% lipid) containing graded levels (0–4 g/kg) of bamboo charcoal powder for eight weeks. There were four tanks (350 L) of fish (initial weight 16.0 ± 0.5 g) for each treatment, and the fish were fed to satiation four times each day. At the end of the feeding trial, all of the fish from each tank were weighed to calculate the growth performance. Blood samples were firstly obtained to collect plasma for the biochemical indexes determination. Liver tissues were then collected to determine the antioxidant enzyme activities and gene expression. Dorsal muscles were also collected to determine the nutrient composition. The results show that when the bamboo charcoal content in the Se-rich feed ranged between 0 and 3 g/kg, the weight growth rate (WGR) and specific growth rate (SGR) values increased with the higher dietary bamboo charcoal content, and the maximum WGR and SGR values were achieved when the bamboo charcoal content in the Se-rich feed was 2–3 g/kg (p < 0.05). The Se content in muscle tissues decreased significantly with the increased bamboo charcoal content (p < 0.05) in the Se-rich feed, which ranged from 0 to 4 g/kg. When the bamboo charcoal content in the Se-rich feed was 2–3 g/kg, the levels of glucose (GLU) and albumin (ALB) in plasma reached a maximum (p < 0.05), whereas the level of alkaline phosphatase (ALP) reached a minimum (p < 0.05). Additionally, the activities of catalase (CAT), total superoxide dismutase (T-SOD), total antioxidative capacity (T-AOC), and glutathione peroxidase (GSH-Px) were significantly enhanced (p < 0.05) when the bamboo charcoal content was 3 g/kg. In contrast, the malondialdehyde (MDA) level increased sharply when the bamboo charcoal content in the Se-rich feed was 1 g/kg, compared to the control group and the groups supplemented with 2–3 g/kg bamboo charcoal (p < 0.05). Regarding mRNA-level gene expression, the results show that dietary supplementation with 0 to 3 g/kg of bamboo charcoal increased the expression of keap1 and nrf2, whereas nfkb expression was inhibited (p < 0.05). The mRNA expression of the antioxidant enzymes cat, gpx, and mn-sod was consistently enhanced in the group fed with the 3 g/kg bamboo charcoal diet (p < 0.05). The expression of the pro-inflammatory cytokines tnfα and tgfβ was inhibited in the groups supplemented with 2–3 g/kg bamboo charcoal, whereas the expression of anti-inflammatory cytokines (il10) increased in the bamboo charcoal supplementation groups compared to the control group (p < 0.05). Generally, supplementation with 2–3 g/kg of bamboo charcoal in Se-rich feed improved the growth performance, physiological status, and antioxidant enzyme activities of blunt snout bream. Moreover, bamboo charcoal supplementation in Se-rich diets stimulated the antioxidant system and inhibited the inflammatory response by activating Nrf2-Keap1 and suppressing NF-κB.

Effects of melatonin implantation on cashmere growth, hormone concentrations and cashmere yield in cashmere-perennial-type Liaoning cashmere goats

2017 ◽  
Vol 57 (1) ◽  
pp. 60 ◽  
Author(s):  
Chunhui Duan ◽  
Jianhai Xu ◽  
Yu Zhang ◽  
Wei Zhang ◽  
Yabo Sun ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Growth Rate ◽  
Fibre Diameter ◽  
Control Group ◽  
Plasma Prolactin ◽  
Blood Samples ◽  
Growing Period ◽  
Plasma Melatonin ◽  
Cashmere Goats ◽  
Slow Growing

The aim of the present study was to investigate the effects of melatonin implants on cashmere growth, the concentrations of plasma melatonin and prolactin and the total cashmere yield in cashmere-perennial-type Liaoning cashmere goats. Twenty female goats were assigned to two treatments (n = 10) including a control and a treatment in which melatonin (2 mg/kg bodyweight) was implanted in March and May, respectively. The experiment lasted for 153 days. Fibre samples were collected in July, August and April the following year (before cashmere harvest). Blood samples were taken monthly from March to August. Cashmere yield was recorded after harvest. In melatonin-treated goats, cashmere length and cashmere growth rate from April to July were significantly increased (P < 0.05), but no influence was observed (P > 0.05) in August. Implantation of melatonin significantly increased plasma melatonin concentrations (P < 0.05) and decreased prolactin concentrations from April to July compared with the control group (P < 0.05), but no difference was observed in August (P > 0.05). Administration of melatonin increased the cashmere yield by 6.2% and the maximum cashmere length by 8.4%, but the differences were not significant (P > 0.05). Moreover, the cashmere fibre diameter was not influenced by melatonin implantation (P > 0.05). The results also indicated that plasma melatonin concentrations were correlated with plasma prolactin in the regulation of cashmere growth. Implantation of melatonin was an effective way to promote cashmere growth, and administration during the cashmere slow-growing period improved cashmere production without changing cashmere fibre diameter in cashmere-perennial-type Liaoning cashmere goats.

scholarly journals The effect of alkaloids present in blue lupine (Lupinus angustifolius) seeds on the growth rate, selected biochemical blood indicators and histopathological changes in the liver of rats

2015 ◽  
Vol 84 (1) ◽  
pp. 55-62 ◽  
Author(s):  
Maria Stanek ◽  
Tadeusz Rotkiewicz ◽  
Wiesław Sobotka ◽  
Jacek Bogusz ◽  
Iwona Otrocka-Domagała ◽  
...  
Keyword(s):  
Growth Rate ◽  
Lupinus Angustifolius ◽  
Control Group ◽  
Histopathological Analysis ◽  
Serum Concentrations ◽  
Feeding Trial ◽  
Initial Body Weight ◽  
Carbohydrate And Lipid Metabolism ◽  
Liver Morphology ◽  
Induced Changes

The objective of this study was to determine the effect of alkaloids present in blue lupine (Lupinus angustifolius) seeds on the growth rate, selected indicators of carbohydrate and lipid metabolism, and liver morphology in rats. The experimental material comprised 32 Wistar rats at around 3 weeks of age, with an initial body weight of 81 g. During a 28-day feeding trial, the rats were fed diets containing the seeds of three blue lupine cultivars, Baron, Zeus and Wersal, with different alkaloid concentrations (0.36, 0.41, 0.56 mg/kg, respectively). Diets containing the seeds of three blue lupine cultivars reduced the feed intake and significantly limited the growth rate of experimental rats, compared to the control group. Lupine alkaloids had no effect on the serum concentrations of glucose and total cholesterol in rats, whereas elevated triglyceride concentrations were noted in experimental groups, relative to the control group. Diets containing the seeds of blue lupine cultivars Zeus and Wersal induced changes in alanine transaminase activity. A histopathological analysis of the liver revealed parenchymatous degeneration, which was more advanced in rats fed diets with the seeds of blue lupine cultivars Zeus and Wersal than in the control group, and congestion of portal vessels, which was more severe in rats fed the seeds of blue lupine cultivars Baron and Zeus.

scholarly journals Melatonin Regulates the Periodic Growth of Cashmere by Upregulating the Expression of Wnt10b and β-catenin in Inner Mongolia Cashmere Goats

2021 ◽  
Vol 12 ◽  
Author(s):  
Junyang Liu ◽  
Qing Mu ◽  
Zhihong Liu ◽  
Yan Wang ◽  
Jiasen Liu ◽  
...  
Keyword(s):  
Hair Follicle ◽  
Inner Mongolia ◽  
Growth Cycle ◽  
Hair Follicles ◽  
Skin Tissue ◽  
Follicle Development ◽  
Periodic Growth ◽  
Cashmere Goats ◽  
Hair Follicle Development ◽  
Hair Follicle Growth

Secondary hair follicle growth in cashmere goats has seasonal cycle changes, and melatonin (MT) has a regulatory effect on the cashmere growth cycle. In this study, the growth length of cashmere was measured by implanting MT in live cashmere goats. The results indicated that the continuous implantation of MT promoted cashmere to enter the anagen 2 months earlier and induce secondary hair follicle development. HE staining of skin tissues showed that the number of secondary hair follicles in the MT-implanted goats was significantly higher than that in the control goats (P &lt; 0.05). Transcriptome sequencing of the skin tissue of cashmere goats was used to identify differentially expressed genes: 532 in February, 641 in October, and 305 in December. Fluorescence quantitative PCR and Western blotting results showed that MT had a significant effect on the expression of Wnt10b, β-catenin, and proteins in the skin tissue of Inner Mongolia cashmere goats. This finding suggested that MT alters the cycle of secondary hair follicle development by changing the expression of related genes. This research lays the foundation for further study on the mechanism by which MT regulates cashmere growth.

scholarly journals Single-Cell Transcriptomics Reveals the Molecular Anatomy of Sheep Hair Follicle Heterogeneity and Wool Curvature

2021 ◽  
Vol 9 ◽  
Author(s):  
Shanhe Wang ◽  
Tianyi Wu ◽  
Jingyi Sun ◽  
Yue Li ◽  
Zehu Yuan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Single Cell ◽  
Hair Follicle ◽  
Molecular Mechanism ◽  
Intercellular Communication ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Follicle Development ◽  
Molecular Anatomy ◽  
Hair Follicle Development

Wool is the critical textile raw material which is produced by the hair follicle of sheep. Therefore, it has important implications to investigate the molecular mechanism governing hair follicle development. Due to high cellular heterogeneity as well as the insufficient cellular, molecular, and spatial characterization of hair follicles on sheep, the molecular mechanisms involved in hair follicle development and wool curvature of sheep remains largely unknown. Single-cell RNA sequencing (scRNA-seq) technologies have made it possible to comprehensively dissect the cellular composition of complex skin tissues and unveil the differentiation and spatial signatures of epidermal and hair follicle development. However, such studies are lacking so far in sheep. Here, single-cell suspensions from the curly wool and straight wool lambskins were prepared for unbiased scRNA-seq. Based on UAMP dimension reduction analysis, we identified 19 distinct cell populations from 15,830 single-cell transcriptomes and characterized their cellular identity according to specific gene expression profiles. Furthermore, novel marker gene was applied in identifying dermal papilla cells isolated in vitro. By using pseudotime ordering analysis, we constructed the matrix cell lineage differentiation trajectory and revealed the dynamic gene expression profiles of matrix progenitors' commitment to the hair shaft and inner root sheath (IRS) cells. Meanwhile, intercellular communication between mesenchymal and epithelial cells was inferred based on CellChat and the prior knowledge of ligand–receptor pairs. As a result, strong intercellular communication and associated signaling pathways were revealed. Besides, to clarify the molecular mechanism of wool curvature, differentially expressed genes in specific cells between straight wool and curly wool were identified and analyzed. Our findings here provided an unbiased and systematic view of the molecular anatomy of sheep hair follicle comprising 19 clusters; revealed the differentiation, spatial signatures, and intercellular communication underlying sheep hair follicle development; and at the same time revealed the potential molecular mechanism of wool curvature, which will give important new insights into the biology of the sheep hair follicle and has implications for sheep breeding.

scholarly journals Effects of Photoperiod Change on Melatonin Secretion, Immune Function and Antioxidant Status of Cashmere Goats

Animals ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 766 ◽  
Author(s):  
Mao ◽  
Xu ◽  
Shi ◽  
Guo ◽  
Jin ◽  
...  
Keyword(s):  
Gene Expression ◽  
Immune Function ◽  
Antioxidant Status ◽  
Interleukin 2 ◽  
Control Group ◽  
Related Factor ◽  
Blood Leukocytes ◽  
Natural Photoperiod ◽  
Cashmere Goats ◽  
Factor Α

The photoperiod affects animals’ secretion of hormones, especially melatonin (MLT), which is involved in the regulation of the immune function and antioxidant status. The present experiment was conducted to study the effects of the photoperiod on MLT secretion, immune function, antioxidant status and related gene expression in goats. Eighteen adult female cashmere goats were randomly divided into three photoperiod groups: the control group (CG: natural photoperiod); the short-day photoperiod group (SDPP group: 8 h light; 16 h dark) and the shortening-day photoperiod group (SIPP group: lighting time shortened gradually from 16 h/d to 8 h/d). The experiment lasted for 60 days. The results showed that SDPP increased MLT concentration in serum at day 30 of the experiment (p < 0.05), but SIPP increased it at day 60 (p < 0.05). The activity of total superoxide dismutase (T-SOD), glutathione peroxidase (GPx) and catalase (CAT) increased (p < 0.05), and malondialdehyde (MDA) concentration decreased (p < 0.05) at day 30 in SDPP; no significant effects of SIPP were observed at day 30. Both SDPP and SIPP goats had higher activities of T-SOD, GPx and CAT (p < 0.05) at day 60. The concentration of immunoglobulin G (IgG), interleukin 1β (IL-1β) and interleukin 2 (IL-2) increased in SDPP (p < 0.05) at day 30. Both SDPP and SIPP raised the concentration of IgG, IL-1β and IL-2 at day 60 (p < 0.05). For the relative gene expression, the SDPP improved the gene expression of SOD1, CAT, GPx4, nuclear factor erythroid-2-related factor 2(Nrf2), IL-1β, IL-2 and tumor necrosis factor-α (TNF-α) (p < 0.05) in blood leukocytes at day 30. In addition, at day 60, goats in the SDPP group had a higher gene expression of CAT, GPx4, IL-1β and IL-2 (p < 0.05). Goats in SIPP had significantly higher gene expression of SOD1, CAT, GPx4, Nrf2, TNFα, IL-1β and IL-2 (p < 0.05) than those in CG. These results indicated that SDPP and SIPP could secrete more MLT and then improve the immune function and antioxidant status of the goats.

scholarly journals High doses of cobalt inhibited hair follicle development in Rex Rabbits

2019 ◽  
Vol 27 (4) ◽  
pp. 217
Author(s):  
L. Liu ◽  
Q. Gao ◽  
C. Wang ◽  
Z. H. Fu ◽  
K. Wang ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Hair Follicle ◽  
Basal Diet ◽  
Equal Treatment ◽  
Control Group ◽  
Follicle Development ◽  
Mrna Levels ◽  
Cobalt Sulfate ◽  
Protein Levels ◽  
Hair Follicle Development

<p>An experiment was conducted to investigate the effect of cobalt supplementation on hair follicle development in rabbits. Rex rabbits (30-d-old, n=180) were divided randomly into five equal treatment groups: rabbits fed a basal diet (control, measured cobalt content of 0.27 mg/kg) or rabbits fed a basal diet with an additional 0.1, 0.4, 1.6 or 6.4 mg/kg cobalt (in the form of cobalt sulfate) supplementation (measured cobalt contents of 0.35, 0.60, 1.83 and 6.62 mg/kg, respectively). Treatment with 6.4 mg/kg cobalt significantly decreased hair follicle density (<em>P</em>&lt;0.05), while low levels of cobalt (0.1-1.6 mg/kg) had no effect on hair follicle density (<em>P</em>&gt;0.05). The addition of dietary cobalt at the highest level examined (6.4 mg/kg) significantly increased the gene expression of bone morphogenetic protein (BMP) 2 and BMP4 in skin tissue (<em>P</em>&lt;0.05), while the mRNA levels of versican, alkaline phosphatase, hepatocyte growth factor, and noggin remained unchanged (<em>P</em>&gt;0.05). Compared with their levels in the control group, dietary cobalt treatment significantly suppressed the protein levels of p-mechanistic target of rapamycin (mTOR) and p-ribosomal protein S6 protein kinase (<em>P</em>&lt;0.05) but did not alter the protein levels of p-AMP-activated protein kinase, Wnt10b or p-β-catenin (<em>P</em>&gt;0.05). In conclusion, cobalt at the highest concentration examined inhibited hair follicle development, which may have involved the mTOR-BMP signalling pathway.</p>

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