Ganoderma lucidum polysaccharides protect against sepsis-induced cardiac dysfunction by activating SIRT1

2021 ◽  
Author(s):  
Zhong-Heng Xu ◽  
Xiao Su ◽  
Geng Yang ◽  
Tao Qin ◽  
Yu Liu
Keyword(s):  
Cardiac Dysfunction ◽  
Ganoderma Lucidum ◽  
Inflammatory Cells ◽  
Protective Role ◽  
Inflammatory Factors ◽  
Control Group ◽  
Tunel Staining ◽  
Inflammatory Factor ◽  
Protective Effects ◽  
Ganoderma Lucidum Polysaccharides

Abstract Objective To investigate whether the silent information regulator 1 (SIRT1) was involved in the protective effects of Ganoderma lucidum polysaccharides (GLP) against sepsis-induced cardiac dysfunction. Methods Lipopolysaccharide (LPS)-induced sepsis model was constructed in C57/BL6J mice. Mice were randomly divided into LPS + GLP + EX-527, LPS + EX-527, LPS + GLP, LPS or control group). The levels of serum inflammatory factor markers were examined by ELISA. H&E staining was performed to assess the inflammation. TUNEL staining and bromodeoxyuridine staining were used to observe cell apoptosis and proliferation, respectively. Expression of apoptosis and proliferation-related proteins was detected by western blot. Key findings GLP treatment could significantly increase the expression of SIRT1, reduce levels of serum inflammatory factors (TNF-α, IL-1α and IL-6) and inflammatory cells in mice heart tissue of sepsis models (all Ps < 0.01). Compared with LPS group, GLP treatment inhibited apoptosis and promoted proliferation of myocardial tissues (all Ps < 0.01). Besides, EX-527 (SIRT1 inhibitor) treatment could partially reverse the protective effects of GLP against sepsis-induced cardiac dysfunction (all Ps < 0.01). Conclusions GLP might play a protective role in sepsis-induced cardiac dysfunction through regulating inflammatory response, apoptosis and proliferation via activating SIRT1. Therefore, GLP is expected to be a probable novel strategy for treatment of sepsis.

Research on the Effect of p35 for Secretion of Proinflammatory Factor and Apoptosis of Chondrocyte in Chondrocytopathic Articular Fluid

2021 ◽  
Vol 11 (8) ◽  
pp. 1649-1654
Author(s):  
LvLin Yang ◽  
Bowen Zhang ◽  
Yuqi Liang ◽  
Gangning Feng ◽  
Xiaoke Shang ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Synovial Fluid ◽  
Intervention Group ◽  
Inflammatory Cells ◽  
Cartilage Tissue ◽  
Control Group ◽  
Inflammatory Factor ◽  
Proinflammatory Factor ◽  
Group B ◽  
Group Control Group

To study on the effect of transcriptional regulation factor as p35 for secretion of proinflammatory factor and apoptosis of chondrocyte in chondrocytopathic articular fluid so as to improve the chondropathy. The fifty SD rats were selected for our study. It was divided into three groups including A group (control group), B group (chondrocytopathic model group of osteoarthritis) and C group (transcriptional regulation factor as p35 intervention group. The samples were collected after intervention in sixteen weeks. The sampling position was cartilage tissue of rat leg. It was adopted for immunohistochemical inspection and histopathology examination. At the same time the synovial fluid was collected. The concentration of TNF-α and IL-6 was detected. And the expression of mRNA in gene related with apoptosis was detected. The chondrocyte morphology of rats in A group was normal. The chondrocyte was damaged and goblet cell was reduced in B group. The infiltrating inflammatory cells in C group were less than in B group from pathological results. And the goblet cells in C group was increased than in B group. The expression of TNF-α, Bax, NF-κB, IL-6: B group > C group > A group. The expression of Bcl-2: A group > C group > B group. The transcriptional regulation factor as p35 related with anti-apoptosis could regulate the level of inflammatory factor as TNF-α and IL-6 in synovial fluid and restrain the lesion and apoptosis of chondrocyte.

scholarly journals Characterization and Hepatoprotections of Ganoderma lucidum Polysaccharides against Multiple Organ Dysfunction Syndrome in Mice

2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Yiwen Zhang ◽  
Yanbo Feng ◽  
Wenshuai Wang ◽  
Le Jia ◽  
Jianjun Zhang
Keyword(s):  
Organ Dysfunction ◽  
Multiple Organ Dysfunction Syndrome ◽  
Ganoderma Lucidum ◽  
Multiple Organ ◽  
Antioxidant Enzyme Activities ◽  
Inflammatory Factor ◽  
Multiple Organ Dysfunction ◽  
Natural Medicine ◽  
Ganoderma Lucidum Polysaccharides ◽  
Anti Inflammatory

Background. The liver is one of the most commonly affected organs in multiple organ dysfunction syndrome (MODS). In recent years, there have been many studies on Ganoderma lucidum polysaccharides (GLP), but the role of GLP in MODS is still unclear. The purpose of this work was to explore the antioxidant, anti-inflammatory, and protective effects of GLP on the liver in MODS model mice. Methods. The characteristic properties of GLP were processed by physicochemical analysis. The MODS models were successfully established with intraperitoneal injection of zymosan in Kunming strain mice. The antioxidant, anti-inflammatory, and hepatoprotective effects of GLP were processed both in vitro and in vivo by evaluating the oxidative parameters, inflammatory factors, and liver pathological observations. Results. The characterization analysis revealed that GLP was a pyranose mainly composed of glucose with the molecular weights (Mw) of 8309 Da. The experimental results proved that GLP had potential hepatoprotection possibly by improving the antioxidant status (scavenging excessive oxygen radicals, increasing the antioxidant enzyme activities, and reducing the lipid peroxide), alleviating the inflammatory response (reducing the inflammatory factor levels), and guaranteeing the liver functions. Conclusions. This research suggested that GLP had the potential to be developed as a natural medicine for the treatment of multiple organ failure.

scholarly journals PO-052 Study on the Expression of Inflammatory Factor, chemotactic factor in the Repair of Skeletal Muscle Contusion in Mic

2018 ◽  
Vol 1 (3) ◽  
Author(s):  
Linlin Zhao ◽  
Weihua Xiao ◽  
Xin Xu
Keyword(s):  
Skeletal Muscle ◽  
Muscle Injury ◽  
Inflammatory Factors ◽  
Control Group ◽  
Inflammatory Factor ◽  
Post Injury ◽  
Skeletal Muscle Contusion ◽  
Chemotactic Factors ◽  
Polymerase Chain ◽  
Gastrocnemius Muscles

Objective To investigate the regulation of muscle inflammatory factors and chemotactic factors during the repair of skeletal muscle contusion in mice. Methods Forty C57 male mice were needed. Eight for control group (C, n=8) and thirty-tow for muscle contusion group (S, n=32). Subsequently, their gastrocnemius muscles were harvested at 0d, 1d, 3d, 7d, 14d after injury. Hematoxylinand eosin (HE) stain were used to assess the changes of muscle morphology. In addition, the gene expression of inflammatory factors and chemotactic factors was analyzed by real-time polymerase chain reaction. Results 1、Morphology of skeletal muscles showed signs of regeneration at 3d post injury. The maximumamount of regeneration muscle fibers appeared during one week post contusion. Two weeks post-injury morphology of myofibers nearly recovered to normal. 2、After skeletal muscle injury, macrophage markers (CD68, CD163, CD206), a variety of inflammatory factors (IL-1, IL-6, IL-10) were up-regulated. 3、chemotactic factors (CCL2, CCL3, CCL5, CCL8, CXCL9, CXCL10, CXCL12, mRNA) were up-regulated。 Conclusions After skeletal muscle contusion, the expression of a variety of chemotactic factors is up-regulated, which promotes macrophage infiltration and produces a variety of inflammatory factors. They may be involved in the inflammatory response and regeneration process after skeletal muscle contusion.

scholarly journals Anti-inflammatory activity of acacia catechu-bark aqueous solution in aspirin induced gastric ulcer in rodents

2021 ◽  
Vol 8 (12) ◽  
pp. 5649
Author(s):  
Uzma Waseem ◽  
Syeda Rizwana Jafri ◽  
Sarah Khalid ◽  
Fauzia Qureshi ◽  
Nadia Majeed ◽  
...  
Keyword(s):  
Gastric Mucosa ◽  
Inflammatory Cells ◽  
Protective Role ◽  
Gastric Injury ◽  
Medical Institute ◽  
Control Group ◽  
Albino Rats ◽  
Anti Inflammatory ◽  
Group B ◽  
Acacia Catechu

Background: Aspirin is amongst the most widely used drugs and has many adverse effects on gastric mucosa. Anti-inflammatory properties of Acacia catechu have been established already. Objective was to evaluate the histopathological changes induced by aspirin in the stomach of albino rats and to assess the protective effect of different doses of Acacia catechu.Methods: Experimental study Postgraduate Medical Institute, Lahore for 21 days. Forty-eight adult albino rats, both males and female, were divided into four groups A, B, C and D randomly; each comprising of 12 rats. Group A was control, group B was given aspirin 100 mg/kg and group C and D were given aspirin 100 mg/kg along with Acacia catechu 250 mg/kg and 500 mg/kg respectively by oral route. The rats from individual group were sacrificed on 3rd day, 7th day and 14th day and stomachs were examined under light microscope to observe the inflammatory cells infiltration.Results: Gross and microscopic findings on days 3, 7 and 14 were similar. Control groups A1, A2 and A3 showed normal healthy gastric mucosa and the least number of inflammatory cells. In group B, aspirin produced ulcerations and linear breaks; with highest inflammatory infiltrates. On microscopic examination, numerous inflammatory cells were noted. Group C and D rats had minimum ulcer index and fewer inflammatory cells.Conclusions: Acacia catechu has protective role against gastric injury by inhibiting inflammation. 

Mammalian Target of Rapamycin (mTOR) Inhibitor Improves Local Immune Microenvironment and Reduces Seizures via Increasing miR-211 Level in Rat Brain

2022 ◽  
Vol 12 (4) ◽  
pp. 841-847
Author(s):  
Meijiao Du ◽  
Zhengmei Wang ◽  
Geng Su ◽  
Yunxia Zhou ◽  
Chuan Luo
Keyword(s):  
Rat Brain ◽  
Brain Tissue ◽  
Mtor Inhibitor ◽  
Symptom Relief ◽  
Mtor Inhibitors ◽  
Inflammatory Factors ◽  
Control Group ◽  
Tunel Staining ◽  
Immune Microenvironment ◽  
Rat Brain Tissue

This study aims to analyze the role of mTOR inhibitor on the expression of miR-211 in rat brain tissue and the biological effect of miR-211 in attenuating seizure. Rats were randomly divided into four groups, and the number of seizures and the duration of single seizure were observed within 24 hours after intervention. The level of miR-211 in brain tissue was detected by RT qPCR, the apoptosis of nerve cells was assessed by TUNEL staining, the level of immune cells was detected by flow cytometry, and the level of serum inflammatory factors was determined by ELISA. The number of seizures and the duration of single seizure in the three groups treated by rapamycin within 24 hours were lower than those in the control group, and the symptom relief in group C was the best. After treatment, the expression level of miR-211 in the brain tissue of epileptic rats increased. TUNEL staining showed that neuronal apoptosis was obvious in epileptic rats. The anti apoptotic ability of group C was the most significant, followed by group D and group B. Compared with group A, the levels of CD3+ cells, CD8+ cells and CD4+/CD25+ cells in brain tissue of group C were decreased, while the levels of IL-2 and IFN-γ were lower in group C than those in control. In group C (n = 5), the levels of CD3+ cells, CD8+ cells and CD4+/CD25+ cells were elevated, and the levels of immune related cytokines IL-2 and IFN-γ were higher than those of rats without miR-211 inhibition. mTOR inhibitors can improve the local immune microenvironment, reduce the release of inflammatory factors, and finally decrease the frequency and duration of seizures by up regulating the level of miR-211 in rat brain tissue.

scholarly journals Protective Effects and Mechanisms of Rosuvastatin on Acute Kidney Injury Induced by Contrast Media in Rats

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Zehui Jiang ◽  
Jun Zhang ◽  
Yuanan Lu
Keyword(s):  
Protective Effect ◽  
Contrast Medium ◽  
Renal Injury ◽  
Intervention Group ◽  
Inflammatory Factors ◽  
Control Group ◽  
Renal Tubules ◽  
Protective Effects ◽  
Sod Activity ◽  
Biochemical Indicators

Objective. To explore the protective effect and mechanism of rosuvastatin on acute renal injury induced by a nonionic hypotonic contrast medium in rats. Methods. Forty-eight healthy adult SD rats were randomly divided into three groups: normal control group (NC); contrast medium control group (CM); and rosuvastatin intervention group (RI). The RI group was intragastrically administered with a 10 mg/kg of rosuvastatin 12 h prior to the contrast exposure. All rats in CM and RI groups were inoculated with 10 mL/kg of chemical (IV) while the same volume of saline for the NC group. At 24 h and 72 h posttreatments, pathomorphological changes of renal tubules were documented, respectively, and several biochemical indicators were tested to assess renal injury of experimental rats. Results. Compared with the CM group, rats in the RI group showed significantly reduced injury of kidneys and decreased levels of biochemical indicators such as blood Scr, blood Cys-C, urine NAG, urine α1-MG, and urine mALB. The serum Hs-CRP in the CM group increased significantly from 24 h to 72 h (p<0.05), but this was not observed in the rats of the RI group. In addition, SOD activity in the RI group was significantly increased (p<0.01) while SOD activity in renal tissue decreased significantly with time in the CM group (p<0.05). Conclusion. Short-term intervention with rosuvastatin can lead to reduced kidney damage associated with the contrast agent by reducing the levels of inflammatory factors and oxidative stress. Thus, rosuvastatin intervention has a protective effect on rats from contrast-induced nephropathy.

scholarly journals BuPiHeWei Decoction Ameliorates 5-Fu-Induced Intestinal Mucosal Injury in the Rats by Regulating the TLR-4/NF-κB Signaling Pathway

2019 ◽  
Vol 2019 ◽  
pp. 1-10
Author(s):  
Zhi-gao Sun ◽  
Ya-zhuo Hu ◽  
Yu-guo Wang ◽  
Jian Feng ◽  
Yong-qi Dou
Keyword(s):  
Body Weight ◽  
Signaling Pathway ◽  
Bacillus Licheniformis ◽  
Mucosal Injury ◽  
Protective Role ◽  
Inflammatory Factors ◽  
Control Group ◽  
Sprague Dawley ◽  
Intestinal Mucosal Injury ◽  
Group 5

BuPiHeWei (BPHW) decoction, a classic Traditional Chinese Medicinal (TCM) prescription, has been widely used in clinical practice to relieve digestive symptoms caused by chemotherapy, such as diarrhea and vomiting. The present study aimed to investigate whether BPHW decoction exerted a protective role in the 5-Fu-induced intestinal mucosal injury in the rats by regulating the mechanisms of TLR-4/NF-κB signaling pathway. There were 35 Sprague Dawley rats randomly divided into four groups: normal control group, 5-Fu group, 5-Fu + BPHW decoction group (10.5 g/kg, for five continuous days), and 5-Fu + Bacillus licheniformis capsule group (0.2 g/kg, for five continuous days). Animal models were established by intraperitoneal injection of 5-Fu (30 mg/Kg, for five consecutive days). At the end of the treatment period, body weight, diarrhea score, and histological examination were examined. Furthermore, the expression of TLR-4/NF-κB pathway was detected to reveal its mechanism. The results showed that BPHW decoction effectively reduced diarrhea score and increased body weight and height of villi after 5-Fu chemotherapy. In addition, BPHW decoction could significantly inhibit the expression of TLR-4, NF-κB, and inflammatory factors (including TNF-α, IL-1β, and IL-6) in the intestine, and the efficacy was significantly higher than that of Bacillus licheniformis capsule. In summary, BPHW decoction might be considered an effective drug to alleviate intestinal mucosal injury in the rats induced by 5-Fu.

Efficacy of Periodontal Tissue Regeneration Combined with Orthodontic Therapy on Periodontitis and Its Influences on Inflammatory Factors in Patients

2020 ◽  
Vol 10 (5) ◽  
pp. 737-742
Author(s):  
Jianxing Han ◽  
Junping Dong ◽  
Hua Zhao ◽  
Yuan Ma ◽  
Shuoran Yang ◽  
...  
Keyword(s):  
Postoperative Complications ◽  
Tissue Regeneration ◽  
Orthodontic Treatment ◽  
Inflammatory Factors ◽  
Control Group ◽  
Inflammatory Factor ◽  
Observation Group ◽  
Periodontal Tissue ◽  
Clinical Attachment Loss ◽  
Periodontal Tissue Regeneration

Objective: To assess the effect of periodontal tissue regeneration combined with orthodontic treatment on periodontitis and inflammatory factors. Methods : 100 patients with periodontitis were randomly separated into observation group and control group. Patients were treated with periodontal tissue regeneration in control group and received orthodontic treatment in observation group. The periodontal indexes, X-ray cephalometric indexes, serum inflammatory factor levels, tooth mobility, the postoperative complications, efficacy and life quality were measured. Results: After treatment, levels of clinical attachment loss (CAL), probing depth (PD), sulcus bleeding index (SBI), gingival index (GI), plaque index (PLI), SNB angle, SNA angle, IL-8, IL-5, TNF-α and hs-CRP of patients in observation group were significantly decreased, while ANB angle was significantly elevated (p < 0 05). Meanwhile, the treatment effective rate and quality of life score was significantly improved after treatment in observation group (p < 0 05). Conclusion: Periodontal tissue regeneration combined with orthodontic treatment can significantly improve periodontitis symptoms, promote the recovery of tooth function, reduce inflammation and postoperative complications, and improve the uniformity and appearance of teeth in patients with periodontitis.

scholarly journals The Effect of Pyrroloquinoline Quinone on the Expression of WISP1 in Traumatic Brain Injury

2017 ◽  
Vol 2017 ◽  
pp. 1-16 ◽  
Author(s):  
Yongqi Ye ◽  
Pengju Zhang ◽  
Yuhang Qian ◽  
Baoxin Yin ◽  
Meijuan Yan
Keyword(s):  
Traumatic Brain Injury ◽  
Brain Injury ◽  
Caspase 3 ◽  
Negative Control Group ◽  
Pyrroloquinoline Quinone ◽  
Protective Role ◽  
Negative Control ◽  
Control Group ◽  
Protective Effects ◽  
Si Rna

WISP1, as a member of the CCN4 protein family, has cell protective effects of promoting cell proliferation and inhibiting cell apoptosis. Although some studies have confirmed that WISP1 is concerned with colon cancer and lung cancer, there is little report about the influence of WISP1 in traumatic brain injury. Here, we found that the expression of WISP1 mRNA and protein decreased at 3 d and then increased at 5 d after traumatic brain injury (TBI). Meanwhile, immunofluorescence demonstrated that there was little colocation of WISP1 with GFAP, Iba1, and WISP1 colocalized with NeuN partly. WISP1 colocalized with LC3, but there was little of colocation about WISP1 with cleaved caspase-3. Subsequent study displayed that the expression ofβ-catenin protein was identical to that of WISP1 after TBI. WISP1 was mainly located in cytoplasm of PC12 or SHSY5Y cells. Compared with the negative control group, WISP1 expression reduced obviously in SHSY5Y cells transfected with WISP1 si-RNA. CCK-8 assay showed that pyrroloquinoline quinone (PQQ) had little influence on viability of PC12 and SHSY5Y cells. These results suggested that WISP1 played a protective role after traumatic brain injury in rats, and this effect might be relative to autophagy caused by traumatic brain injury.

Protective role of silymarin and D-penicillamine against lead-induced liver toxicity and oxidative stress

2017 ◽  
Vol 33 (6) ◽  
pp. 512-518 ◽  
Author(s):  
Seyedeh Missagh Jalali ◽  
Hossein Najafzadeh ◽  
Sadegh Bahmei
Keyword(s):  
Liver Toxicity ◽  
Serum Alkaline Phosphatase ◽  
Protective Role ◽  
Control Group ◽  
Albino Rats ◽  
Protective Effects ◽  
Antioxidant Defence ◽  
Antioxidant Defence System ◽  
Pb Exposure ◽  
Group 2

This study was performed to assess hepatotoxicity and alterations in liver antioxidant defence in acute lead (Pb) exposure and the protective effects of silymarin in comparison to D-penicillamine in rats. Forty eight Albino rats were divided in eight groups and received the following treatments in a 10-day experiment – group 1: normal saline as control; group 2: 25-mg/kg Pb acetate, intraperitoneally (IP) for the last 5 days; group 3: 100-mg/kg D-penicillamine, IP for the last 5 days; group 4: 200-mg/kg silymarin, orally for 10 days; and groups 5, 6, 7 and 8: in addition to Pb, they received D-penicillamine, for the last 5 days, silymarin for 10 days, a combination of silymarin for 10 days and D-penicillamine for the last 5 days and silymarin for the last 5 days, respectively. Pb acetate exposure induced significant elevation in serum alkaline phosphatase (ALP), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) enzyme activities in group 2 compared to control group. Significant reductions in serum total protein and albumin in all Pb-exposed groups and in serum glucose in groups 2, 6 and 8 were also observed. Liver tissue superoxide dismutase and glutathione peroxidase were significantly lower in groups 2 and 8 compared to control group. Silymarin pretreatment and D-penicillamine administration in groups 5, 7 and 8 could significantly lower ALP, ALT and AST and improve liver antioxidant enzymes. Thus, acute Pb exposure induced hepatotoxicity with suppression of liver antioxidant defence system and silymarin, as an antioxidant could alleviate this effect.

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