POLYETHYLENE GLYCOL-FUNCTIONALIZED MAGNETIC (Fe3O4) NANOPARTICLES: A GOOD METHOD FOR A SUCCESSFUL ANTIBACTERIAL THERAPEUTIC AGENT VIA DAMAGE DNA MOLECULE

2019 ◽  
Vol 26 (10) ◽  
pp. 1950079 ◽  
Author(s):  
WALEED K. ABDUL KADHIM ◽  
UDAY M. NAYEF ◽  
MAJID S. JABIR
Keyword(s):  
Cell Wall ◽  
Antibacterial Activity ◽  
Polyethylene Glycol ◽  
Nucleic Acid ◽  
Antibacterial Agent ◽  
Bacterial Species ◽  
Good Method ◽  
Diffusion Method ◽  
Bacterial Cells ◽  
Average Size

Magnetite (Fe3O4) nanoparticles (MPs) capped with polyethylene glycol (PEG) were prepared by a hydrothermal method, and their antibacterial activity was examined against Staphylococcus aureus, Escherichia coli and Psudomonas aeruginosa. The functionalized NPs were characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), vibrating sample magnetometer (VSM), Fourier transform infrared (FTIR) spectroscopy, and Thermogravimetry (TG). The average size of the Fe3O4 was in the range 9–20[Formula: see text]nm, while the functionalized PEG–Fe3O4 had an average size of 5–15[Formula: see text]nm. The PEG–Fe3O4 exhibited superparamagnetism and high saturation magnetization at room temperature. The antibacterial activity of the Fe3O4 and PEG–Fe3O4 were evaluated against E. coli, S. aureus, and P. aeruginosa using the agar well diffusion method. The changes in the morphology of the studied bacterial species were observed via SEM, while the mode of action of the studied agents was determined via the detection of reactive oxygen species (ROS) using Acridine orange-ethidium bromide (AO/EtBr) staining method. The results showed that PEG-functionalized magnetic (Fe3O4) NPs as a novel DNA-mediated antibacterial agent. The PEG–Fe3O4 NPs were observed to destroy the bacterial cells by permeating the bacterial nucleic acid and cytoplasmic membrane, resulting in the loss of cell-wall integrity, nucleic acid damage, and increased cell-wall permeability. The PEG–Fe3O4 NPs could serve as a potential antibacterial agent in future biomedical and pharmaceutical applications.

scholarly journals Nano-curcumin Capped Au/ZnO Nanocomposite: A Promising Approach to Protect from Staphylococcus Aureus Infection through Inhibits Production of α-Hemolysin

2021 ◽  
Author(s):  
Majid Jabir ◽  
Majid Jabir ◽  
Taha M. Rashid ◽  
Uday M. Nayef ◽  
Duha A. Kadhim
Keyword(s):  
Staphylococcus Aureus ◽  
Cell Wall ◽  
Nucleic Acid ◽  
Antibacterial Agent ◽  
Cell Injury ◽  
Cytoplasmic Membrane ◽  
Alveolar Epithelial Cell ◽  
Diffusion Method ◽  
Bacterial Cells ◽  
Zno Nps

Abstract Gold with Zinc Oxide nanoparticles (Au@ZnO NPs) were prepared by laser ablation then capped with Curcumin nanoparticles. The ability of Nano-curcumin-Au/ZnO nanocomposite as a promising antibacterial agent was tested against Staphylococcus aureus. Cur-Au@ZnO NPs were characterized by TEM, FTIR spectroscopy, and Uv-spectroum. TEM image of Au@ZnO NPs has grain size almost 27–38 nm and it increased after capped Nano-curcumin to 72–113 nm. Agar well diffusion method was used to evaluate the antibacterial activity of Cur-Au@ZnO against S.aureus. The activity of Cur-Au@ZnO NPs was determined via detection of (ROS) using (AO/EtBr) staining assay. The bacterial cytoplasmic membrane and nucleic acid were penetrated by tested nanoparticles, resulting in bacterial strain destruction. The results showed that Cur-Au@ZnO NPs as a novel DNA-mediated antibacterial agent. The Cur-Au@ZnO were observed to destroy the bacterial cells by permeating the bacterial nucleic acid and cytoplasmic membrane, resulting in the loss of cell-wall integrity, nucleic acid damage, and increased cell-wall permeability. Furthermore, in the present study we investigated the activity of Cur-Au@ZnO NPs against bacterial α-Hemolysin toxin. Western blot were used to measure the effect of Cur-Au@ZnO NPs on α-Hemolysin produced by S. aureus. The effectiveness of Cur-Au@ZnO NPs against human alveolar epithelial cell injury by α-Hemolysin was tested using live ⁄ dead staining. Also, we demonstrated the role of Cur-Au@ZnO NPs against S. aureus through histopathology examination in a mouse model. Taken together, Cur-Au@ZnO NPs is a potent inhibitor of α-hemolysin secreted by S. aureus. So, Cur-Au@ZnO NPs mediated inhibition of α-Hemolysin production may offer a new strategy in combating pathogen infections. The Cur-Au@ZnO could serve as a potential antibacterial agent in future for biomedical and pharmaceutical applications.

Antibacterial Activity of Thujaoccidentalis,ThujaorientalisandChamaecyparisobtusa

2017 ◽  
Vol 8 (03) ◽  
Author(s):  
Kyoung- Sun Seo ◽  
Seong Woo Jin ◽  
Seongkyu Choi ◽  
Kyeong Won Yun
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antibacterial Agent ◽  
Methanol Extract ◽  
The Other ◽  
Diffusion Method ◽  
E Coli ◽  
Agar Diffusion ◽  
Agar Diffusion Method ◽  
Crude Methanol Extract

The antibacterial activity of three Cupressaceae plants (Thujaoccidentalis,ThujaorientalisandChamaecyparisobtusa) was tested against three bacteria using the agar diffusion method. The ether and ethylacetate fraction of crude methanol extract from the three plants showed potent antibacterial activity against the tested microorganisms. The result showed that Staphylococcus aureus revealed the most sensitivity among the tested bacteria. Thujaoccidentalisether fraction and Thujaorientalis hexane fraction exhibited the highest antibacterial activity against Staphylococcus aureus. E. coli was shown the highest MIC values compared to the other two tested bacteria, which indicates the lowest antibacterial activity against the bacterium. This study promises an interesting future for designing a potentially active antibacterial agent from the three Cupressaceae plants.

Green Synthesis of Silver Nanoparticles Using Artocarpus hirsutus Seed Extract and its Antibacterial Activity

2020 ◽  
Vol 21 (10) ◽  
pp. 980-989
Author(s):  
Sampath Shobana ◽  
Sunderam Veena ◽  
S.S.M. Sameer ◽  
K. Swarnalakshmi ◽  
L.A. Vishal
Keyword(s):  
Silver Nanoparticles ◽  
Cell Wall ◽  
Antibacterial Activity ◽  
Listeria Monocytogenes ◽  
Nanoparticle Synthesis ◽  
Enterobacter Aerogenes ◽  
Seed Extract ◽  
Diffusion Method ◽  
Multi Drug Resistance ◽  
Gastrointestinal Bacteria

Aims: To evaluate the antibacterial activity of Artocarpus hirsutus mediated seed extract for nanoparticle synthesis. Background: Gastrointestinal bacteria are known for causing deadly infections in humans. They also possess multi-drug resistance and interfere with clinical treatments. Applied nanotechnology has been known to combat such infectious agents with little interference from their special attributes. Here we synthesize silver nanoparticles from Artocarpus hirsutus seed extract against two gastro-intestinal bacterial species: Enterobacter aerogenes and Listeria monocytogenes. Objective: To collect, dry, and process seeds of Artocarpus hirsutus for nanoparticle synthesis. To evaluate the morphological interaction of silver nanoparticles with bacteria. Methods: Artocarpus hirsutus seeds were collected and processed and further silver nanoparticles were synthesized by the co-precipitation method. The synthesized nanoparticles were characterized using XRD, UV, FTIR, and SEM. These nanoparticles were employed to study the antibacterial activity of nanoparticles against Enterobacter aerogenes and Listeria monocytogenes using well diffusion method. Further, morphological interaction of silver nanoparticles on bacteria was studied using SEM. Result: Silver nanoparticles were synthesized using Artocarpus hirsutus seed extract and characterization studies confirmed that silver nanoparticles were spherical in shape with 25-40 nm size. Antibacterial study exhibited better activity against Enterobacter aerogenes with a maximum zone of inhibition than on Listeria monocytogenes. SEM micrographs indicated that Enterobacter aerogenes bacteria were more susceptible to silver nanoparticles due to the absence of cell wall. Also, the size and charge of silver nanoparticles enable easy penetration of the bacterial cell wall. Conclusion: In this study, silver nanoparticles were synthesized using the seed extract of Artocarpus hirsutus for the first time exploiting the fact that Moraceae species have high phytonutrient content which aided in nanoparticle synthesis. This nanoparticle can be employed for large scale synthesis which when coupled with the pharmaceutical industry can be used to overcome the problems associated with conventional antibiotics to treat gastrointestinal bacteria.

scholarly journals Polyethylene Glycol Functionalized Graphene Oxide Nanoparticles Loaded with Nigella sativa Extract: A Smart Antibacterial Therapeutic Drug Delivery System

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3067
Author(s):  
Mustafa A. Jihad ◽  
Farah T. M. Noori ◽  
Majid S. Jabir ◽  
Salim Albukhaty ◽  
Faizah A. AlMalki ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Grain Size ◽  
Cell Wall ◽  
Graphene Oxide ◽  
Polyethylene Glycol ◽  
Nucleic Acid ◽  
Drug Delivery System ◽  
Delivery System ◽  
Nigella Sativa ◽  
Seed Extract

Flaky graphene oxide (GO) nanoparticles (NPs) were synthesized using Hummer’s method and then capped with polyethylene glycol (PEG) by an esterification reaction, then loaded with Nigella sativa (N. sativa) seed extract. Aiming to investigate their potential use as a smart drug delivery system against Staphylococcus aureus and Escherichia coli, the spectral and structural characteristics of GO-PEG NPs were comprehensively analyzed by XRD, AFM, TEM, FTIR, and UV- Vis. XRD patterns revealed that GO-PEG had different crystalline structures and defects, as well as a higher interlayer spacing. AFM results showed GONPs with the main grain size of 24.41 nm, while GONPs–PEG revealed graphene oxide aggregation with the main grain size of 287.04 nm after loading N. sativa seed extract, which was verified by TEM examination. A strong OH bond appeared in FTIR spectra. Furthermore, UV- Vis absorbance peaks at (275, 284, 324, and 327) nm seemed to be correlated with GONPs, GO–PEG, N. sativa seed extract, and GO –PEG- N. sativa extract. The drug delivery system was observed to destroy the bacteria by permeating the bacterial nucleic acid and cytoplasmic membrane, resulting in the loss of cell wall integrity, nucleic acid damage, and increased cell-wall permeability.

scholarly journals Evaluating Flinders Technology Associates card for transporting bacterial isolates and retrieval of bacterial DNA after various storage conditions

2020 ◽  
Vol 13 (10) ◽  
pp. 2243-2251
Author(s):  
Azhar G. Shalaby ◽  
Neveen R. Bakry ◽  
Abeer A. E. Mohamed ◽  
Ashraf A. Khalil
Keyword(s):  
Nucleic Acid ◽  
Bacterial Species ◽  
Storage Conditions ◽  
Animal Origin ◽  
Cell Detection ◽  
Bacterial Cells ◽  
Gram Positive ◽  
Bacterial Dna ◽  
Gram Negative ◽  
Fta Cards

Background and Aim: Flinders Technology Associates (FTA) cards simplify sample storage, transport, and extraction by reducing cost and time for diagnosis. This study evaluated the FTA suitability for safe transport and storage of Gram-positive and Gram-negative bacterial cells of animal origin on its liquid culture form and from organ impression smears (tissues) under the same routine condition of microbiological laboratory along with detecting their nucleic acid over different storage conditions. Materials and Methods: Increase in bacterial count from 104 to 107 (colony-forming units/mL) of 78 isolates representing seven bacterial species was applied onto cards. FTA cards were grouped and inoculated by these bacteria and then stored at different conditions of 24-27°C, 4°C, and –20°C for 24 h, for 2 weeks, for 1 and 3 month storage, respectively. Bacteriological examination was done, after which bacterial DNA was identified using specific primers for each bacterial type and detected by polymerase chain reaction (PCR). Results: The total percentage of recovered bacteria from FTA cards was 66.7% at 24-27–C for 24 h, the detection limit was 100% in Gram-positive species, while it was 57.4% in Gram-negative ones. Regarding viable cell detection from organ impression smears, it was successful under the previous conditions. No live bacterial cells were observed by bacteriological isolation rather than only at 24-27°C for 24 h storage. All bacterial DNA were sufficiently confirmed by the PCR technique at different conditions. Conclusion: Overall, the FTA card method was observed to be a valid tool for nucleic acid purification for bacteria of animal origin in the form of culture or organ smears regardless of its Gram type and is used for a short time only 24 h for storage and transport of live bacteria specifically Gram-positive type. Moreover, the bacterial nucleic acid was intact after storage in –20°C for 3 months and was PCR amplifiable.

scholarly journals Factors That Affect the Enlargement of Bacterial Protoplasts and Spheroplasts

2020 ◽  
Vol 21 (19) ◽  
pp. 7131
Author(s):  
Hiromi Nishida
Keyword(s):  
Cell Wall ◽  
Plasma Membrane ◽  
Bacterial Cell ◽  
Metal Ion ◽  
Bacterial Species ◽  
Bacterial Cells ◽  
Cell Enlargement ◽  
Ion Composition ◽  
Gene Manipulation ◽  
Peptidoglycan Biosynthesis

Cell enlargement is essential for the microinjection of various substances into bacterial cells. The cell wall (peptidoglycan) inhibits cell enlargement. Thus, bacterial protoplasts/spheroplasts are used for enlargement because they lack cell wall. Though bacterial species that are capable of gene manipulation are limited, procedure for bacterial cell enlargement does not involve any gene manipulation technique. In order to prevent cell wall resynthesis during enlargement of protoplasts/spheroplasts, incubation media are supplemented with inhibitors of peptidoglycan biosynthesis such as penicillin. Moreover, metal ion composition in the incubation medium affects the properties of the plasma membrane. Therefore, in order to generate enlarged cells that are suitable for microinjection, metal ion composition in the medium should be considered. Experiment of bacterial protoplast or spheroplast enlargement is useful for studies on bacterial plasma membrane biosynthesis. In this paper, we have summarized the factors that influence bacterial cell enlargement.

Study of Antibacterial Activity of Nanosilver-Polypropylene Composite against Contaminated Bacteria in Molasses

2018 ◽  
Vol 939 ◽  
pp. 163-169
Author(s):  
Kittiya Pongsapasiritat ◽  
Nongnapat Jumnienkul ◽  
Rapeepun Dangtungee ◽  
Malinee Sriariyanun
Keyword(s):  
Antibacterial Activity ◽  
Antibacterial Agent ◽  
Bacterial Species ◽  
Antibacterial Activities ◽  
Research Field ◽  
Total Sugar ◽  
Bioethanol Production ◽  
Polypropylene Composite ◽  
Nanosilver Particles ◽  
Sugar Degradation

The nanosilver particles are attractive in the research field of nanotechnology due to one of their applications as an effective antibacterial agent. The main objective of the current research is to inhibit the bacterial growth in the molasses to decrease the rate of total sugar degradation caused by contaminated bacteria. The polypropylene masterbatch containing with AgNPs (PP/AgNPs) was prepared and tested against contaminated bacterial species identified in molasse. The percentage of bacterial inhibition was calculated by observing the viability of bacteria cultures by using spread plate technique after treated with PP/AgNPs masterbatch in batch experiment. The concentrations of nanoparticles in masterbatch were varied to be 200, 500, 1000, 1200 and 1500 ppm. At 1500 ppm, the compromised antibacterial activities were observed in all tested bacterial species with 2-h-long exposure time. The results in this study could be further applied to fit in operation of molasse use in industrial scale in various application, including bioethanol production.

scholarly journals Synthesis of a New Conjugates of Imidazole wih Beta Lactam Moiety and Evaluation of its Expanded Antibacterial Activity

2018 ◽  
Vol 34 (5) ◽  
pp. 2495-2501 ◽  
Author(s):  
Sarmd D. Noori ◽  
Mazin N. Mousa ◽  
Shaker A. N. Al-Jadaan
Keyword(s):  
Antibacterial Activity ◽  
Chemical Structure ◽  
Bacterial Species ◽  
Anaerobic Bacteria ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Beta Lactam ◽  
Disc Diffusion Method ◽  
E Coli ◽  
Ft Ir

Five compounds containing (2,4,5-triphenyl triphenyl-1H-) and azetidinone (beta-lactam) moiety were synthesized. The physical data and yield of synthesized compounds were recorded, the chemical structure of prepared compounds were characterized using FT-IR, 1H-NMR and elemental analysis. The antibacterial activity was evaluated using disc diffusion method that involve tow Gram positive (staph. aureus, E. Faecalis), two Gram negative (E. coli and ‎K. pneumoniae), and one anaerobic bacteria (streptococcus. Pyogen). Different concentration of the prepared compounds has been used, and the obtained result were compared with standard (ceftazidime). Compound (5c) showed the best antibacterial activity against all bacterial species while 5a and 5e does not. Other compounds showed activity against some species.

scholarly journals Combined activity of Azadirachta indica and Dodonea viscose leaves extract on some selected pathogens

2020 ◽  
Vol 12 (1) ◽  
pp. 31-38
Author(s):  
A. I Isah ◽  
A.I. Aminu
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Petroleum Ether ◽  
Azadirachta Indica ◽  
Pathogenic Bacteria ◽  
Bacterial Species ◽  
Plant Secondary Metabolites ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Plant Materials

The study was undertaken to investigate the antimicrobial activity both individually and in combination of two Nigerian plants (Azadirachta indica and Dodonea viscosa) against a variety of pathogenic bacteria. Five bacterial species namely; Staphylococcus aureus, Escherichia coli, Salmonella typhi, Klebsiella pneumoniae and Pseudomonas aeruginosa were selected for the assays. The powdered plant materials of the two plants were extracted using sohxlet extraction technique with methanol water and petroleum ether as solvents. The crude extracts of the two plants were subjected to phytochemical screening for qualitative detection of plant secondary metabolites. The extracts were further tested for antibacterial activity against the selected pathogens singly and then combined using agar well diffusion method. Antibacterial activity of the two plants singly indicates that the methanolic extract possess the highest antibacterial activity at a concentration of 50mg/ml with an inhibition zone of 23.3±0.5mm compared to aqueous extract with 21.7±0.5mm inhibition zone at a concentration of 50mg/ml. The least activity was observed with the petroleum ether extract with an inhibition zone of 7.3±0.5mm at a concentration of 12.5mg/ml. Combination of the plants extracts exhibited lower antibacterial activity on the test isolates compared to single plants as evidenced by the production of lower inhibition zones. Key words: Azadirachta indica, Dodonea viscosa, pathogens, antimicrobial activity, synergistic effect

scholarly journals UJI AKTIVITAS ANTI BAKTERI ASAP CAIR YANG BERASAL DARI BATANG KAYU MANIS DAN KULIT KACANG TANAH

2015 ◽  
Vol 2 (2) ◽  
pp. 190
Author(s):  
Yefrida ◽  
Farrah Aprilina ◽  
Indri Ticel Leone ◽  
Refilda
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antibacterial Agent ◽  
Diffusion Method ◽  
Liquid Smoke ◽  
Growth And Reproduction

 ABSTRAK Antibacterial agent is a compound that prevent the growth and reproduction of bacteria. Antibacterial activity of liquid smoke is caused by acid and fenolic compound. In this research, we determined antibacterial activity of liquid smoke from stem sweet wood  and nut shell by using diffusion method. Both of liquid smoke have antibacterial activity to Staphylococcus aureus  Eschericia coli population. Their activity almost the same to chloramphenicol, but less than formalin. Keywords : antibacterial activity, liquid smoke, diffusion method    

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