scholarly journals LC-MS/MS Identification of a Bromelain Peptide Biomarker fromAnanas comosusMerr

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Eric R. Secor ◽  
Steven M. Szczepanek ◽  
Anurag Singh ◽  
Linda Guernsey ◽  
Prabitha Natarajan ◽  
...  
Keyword(s):  
Protease Inhibitors ◽  
Clinical Utility ◽  
Cysteine Proteinase ◽  
Raw Material ◽  
Clinical Use ◽  
Spectral Count ◽  
Mouse Plasma ◽  
Sds Page ◽  
First Time ◽  
Over 40 Years

Bromelain (Br) is a cysteine peptidase (GenBank AEH26024.1) from pineapple, with over 40 years of clinical use. The constituents mediating its anti-inflammatory activity are not thoroughly characterized and no peptide biomarker exists. Our objective is to characterize Br raw material and identify peptides in the plasma of Br treated mice. After SDS-PAGE in-gel digestion, Br (VN#3507; Middletown, CT, USA) peptides were analyzed via LC/MS/MS using 95% protein probability, 95% peptide probability, and a minimum peptide number = 5. Br spiked mouse plasma (1 ug/ul) and plasma from i.p. treated mice (12 mg/kg) were assessed using SRM. In Br raw material, we identified seven proteins: four proteases, one jacalin-like lectin, and two protease inhibitors. In Br spiked mouse plasma, six proteins (ananain, bromelain inhibitor, cysteine proteinase AN11, FB1035 precursor, FBSB precursor, and jacalin-like lectin) were identified. Using LC/MS/MS, we identified the unique peptide, DYGAVNEVK, derived from FB1035, in the plasma of i.p. Br treated mice. The spectral count of this peptide peaked at 6 hrs and was undetectable by 24 hrs. In this study, a novel Br peptide was identified in the plasma of treated mice for the first time. This Br peptide could serve as a biomarker to standardize the therapeutic dose and maximize clinical utility.

scholarly journals Determination of Essential Oil Bioactive Components and Rosmarinic Acid of Salvia officinalis Cultivated under Different Intra-row Spacing

2013 ◽  
Vol 5 (2) ◽  
pp. 198-203 ◽  
Author(s):  
Mohammad ABU DARWISH ◽  
Ezz Al-Dein AL-RAMAMNEH ◽  
Ivan SALAMON ◽  
Ziad ABU-DIEYEH ◽  
Mohamed AL NAWAISEH ◽  
...  
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Rosmarinic Acid ◽  
Plant Density ◽  
Salvia Officinalis ◽  
Raw Material ◽  
Row Spacing ◽  
Human Disorders ◽  
Tea Production ◽  
First Time

Salvia officinalis, known also as sage, is a medicinal plant belonging to the Lamiaceae family that spreads all over the word in several countries. The demand for the raw material and extracts of this plant is increasing due to its numerous applications in pharmacy, food and herbal tea production. The present study investigated for the first time the effect of 15, 30 and 45 cm intra-row spacing (plant density) on the main constituents of sage essential oils and rosmarinic acid content. The highest content of essential oils (2.7%) and rosmarinic acid (2.0%) were obtained in plants grown using 15 cm planting space. Likewise, close spacing resulted also in a substantial content of 1,8-cineole (47-50%, GC/FID; 55-60%, GC/MS). This work indicated that 1,8-cineole chemotype was a dominant character of cultivated S. officinalis in south of Jordan. In general, the percent of α-thujone in essential oil was not affected by intra-row spacing. However, the percent of β-thujone decreased from (2-3%, GC/MS) in plants grown using 15 cm intra-row spacing to (1-2%, GC/MS) in plants grown using 30 and 45 cm intra-row spacing. The highest content of α-and β-pinene was recorded in plants grown using 45 cm planting space (8-10%, GC/FID; 5-6% GC/MS). Based on GC/MS, camphor compound was enriched (9-10%) in sage plants grown under 15 cm spacing and greater than in plants grown under 30 (6-7%) or 45 cm (5-6%) spacing. The results make the potential use of sage extracts in the treatment of some human disorders or illness an area of further research.

scholarly journals A Novel Approach to Fabricate Foam Ceramics from Steel Slag

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Yu Zheng ◽  
Xudong Luo ◽  
Jinlong Yang ◽  
Wenlong Huo ◽  
Chi Kang
Keyword(s):  
Compressive Strength ◽  
Ph Value ◽  
Steel Slag ◽  
Raw Material ◽  
Solid Loading ◽  
Insulation Material ◽  
Ceramic Foams ◽  
Novel Approach ◽  
The Stability ◽  
First Time

A novel approach is used for fabricating steel slag foam ceramics based on the particle-stabilized foaming method. In this work, steel slag was used as the raw material and propyl gallate (PG) was used as the surface modifier. For the first time, steel slag ceramic foams were successfully fabricated based on particle-stabilized foams. The results show that the stability of the ceramic foams was closely related to the pH value and PG concentration. The porosity and compressive strength could be controlled by changing the solid loading of steel slag and sintering temperature. The porosity of steel slag foam ceramics ranged from 85.6% to 62.53%, and the compressive strength was from 1.74 MPa to 10.42 MPa. The thermal conductivity of steel slag foam ceramics was only 0.067 W (m·K)−1, which shows that it could be used as a thermal insulation material.

Possible Causes in Breaking of Dental Implants Research

2017 ◽  
Vol 907 ◽  
pp. 104-118
Author(s):  
Maria Stoicănescu ◽  
Eliza Buzamet ◽  
Dragos Vladimir Budei ◽  
Valentin Craciun ◽  
Roxana Budei ◽  
...  
Keyword(s):  
Dental Implants ◽  
Dental Implant ◽  
Scientific Literature ◽  
Surface Characteristics ◽  
Raw Material ◽  
New Materials ◽  
Materials Used ◽  
Type Of Failure ◽  
First Time ◽  
Consequent Increase

Dental implants are becoming increasingly used in current dental practice. This increased demand has motivated manufacturers to develop varieties of product through design, but also looking for new materials used to improve surface characteristics in order to obtain a better osseointegration. But the increase in the use of implants goes to a consequent increase in the number of failures. These failures are caused either by treatment complications (peri-implantitis), by fatigue breakage under mechanical over-stress, by defective raw material, or due to errors during the insertion procedures. Although they are rare, these complications are serious in dentistry. Before to market a dental implant to clinical practitioners, the product is validated among other determinations in number of biocompatibility research. Raw material issues, details about its structure and properties are less published by the scientific literature, but all this are subject of a carefully analysis of the producers. Breaking of dental implants during surgical procedures, during the prosthetic procedures or during use (chewing, bruxism, accidents, etc.), is the second most common cause of loss of an implant after consecutive peri-implantitis rejection. Although the frequency of this type of failure for a dental implant is much smaller than those caused by the peri-implantitis, a detailed study of broken implants can explain possible causes. The use of scanning electron microscopy (SEM) in the study of the cleave areas explain the production mechanism of cleavages, starting from micro-fissures in the alloy used for the production of dental implants. These micro-fissures in weak areas of the implant (anti-rotational corners of the polygons, etc.) could generate a serious risk of cleavage first time when a higher force is applied.

Comparative Analysis of the Proteomic Profile of Cattle Hides that Produce Loose and Tight Leather using In-Gel Tryptic Digestion followed by LC-MS/MS

2020 ◽  
Vol 115 (11) ◽  
pp. 399-408
Author(s):  
Catherine Maidment ◽  
Meekyung Ahn ◽  
Rafea Naffa ◽  
Trevor Loo ◽  
Gillian Norris
Keyword(s):  
Type I Collagen ◽  
Raw Material ◽  
Type I ◽  
Collagen Structure ◽  
Proteomic Profile ◽  
Collagen Network ◽  
Collagen Concentration ◽  
Different Types ◽  
Molecular Components ◽  
First Time

Looseness is a defect found in leather that reduces its quality by causing a wrinkly appearance in the finished product, resulting in a reduction in its value. Earlier studies on loose leather using microscopy and Raman spectroscopy reported a change in the collagen structure of loose leather. In this study, proteomics was used to investigate the possible molecular causes of looseness in the raw material, the first time such a study has been carried out. Proteins extracted from two regions of raw hide using two different methods were analysed; those taken from the distal axilla, an area prone to looseness, and those taken from the backbone which is less prone to looseness. Analyses using 1DE-LC-MS/MS showed that although the overall collagen concentration was similar in both areas of the hide, the distribution of the different types of collagen differed.  Specifically, concentrations of type I collagen, and the collagen-associated proteoglycan decorin were lower in samples taken from the distal axilla, symptomatic of a collagen network with excess space seen for these samples using confocal microscopy. This study suggests a possible link between the molecular components of raw cattle hide and looseness and more importantly between the molecular components of skin and skin defects. There is therefore potential to develop biomarkers for looseness which will enable early preventative action.

scholarly journals New data on flint processing of East Trypillia tribes of the Bug-Dnieper interfluve

Vita Antiqua ◽  
2021 ◽  
pp. 113-128
Author(s):  
Ye.V. Pichkur ◽  
Keyword(s):  
Raw Materials ◽  
Raw Material ◽  
Production Waste ◽  
The West ◽  
Quantitative Ratio ◽  
Lithic Industry ◽  
Modern Level ◽  
First Time ◽  
Local Nature

For the first time, materials of such settlements of the East Trypillia culture as Trostyanchyk, Onopriyivka І are published. Despite approximately the same quantitative ratio, even at first glance, the materials of these two leaflets differ significantly from each other. The Trostyanchyk complex is actually blade-type: blades prevail both among production waste and among the tool kit. Flakes and tools on them dominate in Onopriyivka. If the Trostyanchyk complex can be regarded as "classic" in terms of the tool kit: retouched blades, end-scrapers, sickle inserts, perforators on blades, etc., then the Onopriyivka complex looks more primitive: retouched flakes, notched tools and scrapers on flakes, and others. In Onopriyivka, unlike Trostyanchyk, finds of weapons are completely absent. Products from Onopriyivka are made mainly of local raw materials, while in Trostyanchyk there are approximately equal parts of products from local and imported raw materials, and products from local flint are made as carefully as products from Volyn flint. At the same time, in both cases we can confidently speak of the local nature of production. This is evidenced by both the use of local flint raw material and the specific items present in both collections. In Trostyanchyk, as already mentioned, a hammerstone was found, in Onopriyivka — core-like fragments and chips of modify of cores. Such differences can be explained by both territorial and chronological discrepancies. Onopriyivka I is earlier, refers to the end of stage ВІ, Trostyanchyk — to the end of stage ВІІ. Trostyanchyk is located on the Southern Bug, Onopriyivka — in the Bugo-Dnieper interfluve. Although, perhaps, this situation is explained by the unevenness of the study of these sites. In addition, materials from the Vladyslavchyk settlement are published for the first time. Based on their analysis, using analogies from related and synchronous sites, the author tried to identify the features of the East Trypillia lithic industry in the Bug-Dnieper rivers interfluve. As it turned out, at the modern level, the identification of such features is not possible. The materials of the East Trypillia culture of the region are, on the whole, more similar to the materials of the settlements of the West Trypillia culture, than to related sites on the Southern Bug. Keywords: East Trypillia culture, Kukutenʹ-Trypillya, production, flint processing, Bug-Dnieper interfluve

scholarly journals Flowers of Campanula species as a source of biologically active substances

2021 ◽  
Vol 38 ◽  
pp. 00033
Author(s):  
Tatiana Fomina ◽  
Tatiana Kukushkina
Keyword(s):  
Ascorbic Acid ◽  
Western Siberia ◽  
Food Additives ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Raw Material ◽  
Forest Steppe ◽  
Wet Weight ◽  
First Time ◽  
Active Substances

The content of the major groups of biologically active substances in flowers of 7 Campanula species, cultivated in the forest-steppe of Western Siberia, were investigated for the first time. Freshly collected flowers were found to contain 0.06-0.15 % of catechins, 0.31-3.17 % of flavonols, 3.31-6.47 % of tannins, 3.10-15.57 % of pectic substances, and 0.66-16.4 mg% of carotenoids calculated per absolute dre weight of raw material. The amount of ascorbic acid was 36.9-114.0 mg% per wet weight. The data indicate that Campanula flowers are promising for the production of phenolic compounds, the development of food additives and fresh consumptions a seasoning.

scholarly journals Characterization of a Robust and pH-Stable Tannase from Mangrove-Derived Yeast Rhodosporidium diobovatum Q95

Marine Drugs ◽  
2020 ◽  
Vol 18 (11) ◽  
pp. 546
Author(s):  
Jie Pan ◽  
Ni-Na Wang ◽  
Xue-Jing Yin ◽  
Xiao-Ling Liang ◽  
Zhi-Peng Wang
Keyword(s):  
Gallic Acid ◽  
Tannic Acid ◽  
Specific Activity ◽  
Maximum Activity ◽  
Sds Page ◽  
Rhodosporidium Diobovatum ◽  
Bacteria And Fungi ◽  
Ph Range ◽  
First Time

Tannase plays a crucial role in many fields, such as the pharmaceutical industry, beverage processing, and brewing. Although many tannases derived from bacteria and fungi have been thoroughly studied, those with good pH stabilities are still less reported. In this work, a mangrove-derived yeast strain Rhodosporidium diobovatum Q95, capable of efficiently degrading tannin, was screened to induce tannase, which exhibited an activity of up to 26.4 U/mL after 48 h cultivation in the presence of 15 g/L tannic acid. The tannase coding gene TANRD was cloned and expressed in Yarrowia lipolytica. The activity of recombinant tannase (named TanRd) was as high as 27.3 U/mL. TanRd was purified by chromatography and analysed by SDS-PAGE, showing a molecular weight of 75.1 kDa. The specific activity of TanRd towards tannic acid was 676.4 U/mg. Its highest activity was obtained at 40 °C, with more than 70% of the activity observed at 25–60 °C. Furthermore, it possessed at least 60% of the activity in a broad pH range of 2.5–6.5. Notably, TanRd was excellently stable at a pH range from 3.0 to 8.0; over 65% of its maximum activity remained after incubation. Besides, the broad substrate specificity of TanRd to esters of gallic acid has attracted wide attention. In view of the above, tannase resources were developed from mangrove-derived yeasts for the first time in this study. This tannase can become a promising material in tannin biodegradation and gallic acid production.

scholarly journals Magnetic Properties of SmCo5 + 10 wt% Fe Exchange-Coupled Nanocomposites Produced from Recycled SmCo5

Nanomaterials ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 1308 ◽  
Author(s):  
Arnab Chakraborty ◽  
Răzvan Hirian ◽  
Gregor Kapun ◽  
Viorel Pop
Keyword(s):  
Heat Treatment ◽  
Exchange Coupling ◽  
Permanent Magnets ◽  
High Energy ◽  
Raw Material ◽  
Energy Ball ◽  
Hydrogen Decrepitation ◽  
Energy Products ◽  
First Time ◽  
Recycled Material

Nanostructured alloy powders of SmCo5 + 10 wt% Fe obtained using recycled material were studied for the first time. The SmCo5 precursor was obtained from commercial magnets recycled by hydrogen decrepitation. The results were compared with identically processed samples obtained using virgin SmCo5 raw material. The samples were synthesized by dry high-energy ball-milling and subsequent heat treatment. Robust soft/hard exchange coupling was observed—with large coercivity, which is essential for commercial permanent magnets. The obtained energy products for the recycled material fall between 80% and 95% of those obtained when using virgin SmCo5, depending on milling and annealing times. These results further offer viability of recycling and sustainability in production. These powders and processes are therefore candidates for the next generation of specialized and nanostructured exchange-coupled bulk industrial magnets.

Clinical utility of biochemical analysis of cerebrospinal fluid

1995 ◽  
Vol 41 (3) ◽  
pp. 343-360 ◽  
Author(s):  
M A Watson ◽  
M G Scott
Keyword(s):  
Cerebrospinal Fluid ◽  
Clinical Utility ◽  
Biochemical Analysis ◽  
Clinical Chemistry ◽  
Chemistry Laboratory ◽  
Microbial Culture ◽  
Clinical Use ◽  
Clinical Value ◽  
Clinical Chemistry Laboratory ◽  
Biochemical Measurements

Abstract In addition to microbial culture, cytology, and immunological studies, physicians rely on the clinical chemistry laboratory for biochemical analysis of patients' cerebrospinal fluid (CSF). However, apart from routine glucose and protein determinations, the clinical value of other CSF analytes is often unclear. Here, we review the literature pertaining to the use of CSF biochemical measurements in managing patients with infectious disease, neoplasia, stroke and trauma, and dementia. Although a small number of studies demonstrate potential usefulness of some markers, we conclude that, without further study, the data are insufficient to support the routine clinical use of most of the analytes examined.

scholarly journals Production of Cellulosic Ethanol from Enzymatically Hydrolysed Wheat Straws

2020 ◽  
Vol 10 (21) ◽  
pp. 7638
Author(s):  
Vasile-Florin Ursachi ◽  
Gheorghe Gutt
Keyword(s):  
Enzymatic Hydrolysis ◽  
Wheat Straw ◽  
Cellulosic Ethanol ◽  
Raw Material ◽  
High Yield ◽  
Liquid Ratio ◽  
Hydrolysis Process ◽  
Pretreatment Conditions ◽  
Pretreated Wheat Straw ◽  
First Time

The aim of this study is to find the optimal pretreatment conditions and hydrolysis in order to obtain a high yield of bioethanol from wheat straw. The pretreatments were performed with different concentrations of sulphuric acid 1, 2 and 3% (v/v), and were followed by an enzymatic hydrolysis that was performed by varying the solid-to-liquid ratio (1/20, 1/25 and 1/30 g/mL) and the enzyme dose (30/30 µL/g, 60/60 µL/g and 90/90 µL/g Viscozyme® L/Celluclast® 1.5 L). This mix of enzymes was used for the first time in the hydrolysis process of wheat straws which was previously pretreated with dilute sulfuric acid. Scanning electron microscopy indicated significant differences in the structural composition of the samples because of the pretreatment with H2SO4 at different concentrations, and ATR-FTIR analysis highlighted the changes in the chemical composition in the pretreated wheat straw as compared to the untreated one. HPLC-RID was used to identify and quantify the carbohydrates content resulted from enzymatic hydrolysis to evaluate the potential of using wheat straws as a raw material for production of cellulosic ethanol in Romania. The highest degradation of lignocellulosic material was obtained in the case of pretreatment with 3% H2SO4 (v/v), a solid-to-liquid ratio of 1/30 and an enzyme dose of 90/90 µL/g. Simultaneous saccharification and fermentation were performed using Saccharomyces cerevisiae yeast, and for monitoring the fermentation process a BlueSens equipment was used provided with ethanol, O2 and CO2 cap sensors mounted on the fermentation flasks. The highest concentration of bioethanol was obtained after 48 h of fermentation and it reached 1.20% (v/v).

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