scholarly journals Antibacterial and Antioxidant Effects of Magnesium Alloy on Titanium Dental Implants

2022 ◽  
Vol 2022 ◽  
pp. 1-9
Author(s):  
Yang Bai ◽  
Lin Wang ◽  
Lisheng Zhao ◽  
E. Lingling ◽  
Shuo Yang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Properties ◽  
Antibacterial Properties ◽  
Mg Alloy ◽  
Significant Difference ◽  
Improved Stability ◽  
New Type ◽  
The Stability ◽  
Implant Restoration ◽  
Antioxidant Effects

Objectives. In this study, a new type of dental implant by covering the surface of the titanium (Ti) implant with zinc-magnesium (Zn-Mg) alloy was designed, to study the antibacterial and antioxidant effects of Mg alloy on titanium (Ti) implants in oral implant restoration. Methods. Human gingival fibroblasts (HGFs), S. sanguinis, and F. nucleatum bacteria were used to detect the bioactivity and antibacterial properties of Mg alloy-coated Ti implants. In addition, B6/J mice implanted with different materials were used to further detect their antibacterial and antioxidant properties. Results. The results showed that Mg alloy could better promote the adhesion and proliferation and improve the alkaline phosphatase (ALP) activity of HGFs, which contributed to better improved stability of implant osseointegration. In addition, Mg alloy could better inhibit the proliferation of S. sanguinis, while no significant difference was found in the proliferation of F. nucleatum between the two implants. In the mouse model, the peripheral inflammatory reaction and oxidative stress of the Mg alloy implant were significantly lower than those of the Ti alloy implant. Conclusions. Zn-Mg alloy-coated Ti implants could better inhibit the growth of Gram-positive bacteria in the oral cavity, inhibit oxidative stress, and facilitate the proliferation activity of HGFs and the potential of osteoblast differentiation, thus, better increasing the stability of implant osseointegration.

scholarly journals Novel Antioxidant Properties of Doxycycline

2018 ◽  
Vol 19 (12) ◽  
pp. 4078 ◽  
Author(s):  
Dahn Clemens ◽  
Michael Duryee ◽  
Cleofes Sarmiento ◽  
Andrew Chiou ◽  
Jacob McGowan ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Hydrogen Peroxide ◽  
Chronic Inflammation ◽  
Antioxidant Properties ◽  
Antibacterial Properties ◽  
Reactive Oxygen ◽  
Protein Adducts ◽  
Oxygen Species ◽  
Free System

Doxycycline (DOX), a derivative of tetracycline, is a broad-spectrum antibiotic that exhibits a number of therapeutic activities in addition to its antibacterial properties. For example, DOX has been used in the management of a number of diseases characterized by chronic inflammation. One potential mechanism by which DOX inhibits the progression of these diseases is by reducing oxidative stress, thereby inhibiting subsequent lipid peroxidation and inflammatory responses. Herein, we tested the hypothesis that DOX directly scavenges reactive oxygen species (ROS) and inhibits the formation of redox-mediated malondialdehyde-acetaldehyde (MAA) protein adducts. Using a cell-free system, we demonstrated that DOX scavenged reactive oxygen species (ROS) produced during the formation of MAA-adducts and inhibits the formation of MAA-protein adducts. To determine whether DOX scavenges specific ROS, we examined the ability of DOX to directly scavenge superoxide and hydrogen peroxide. Using electron paramagnetic resonance (EPR) spectroscopy, we found that DOX directly scavenged superoxide, but not hydrogen peroxide. Additionally, we found that DOX inhibits MAA-induced activation of Nrf2, a redox-sensitive transcription factor. Together, these findings demonstrate the under-recognized direct antioxidant property of DOX that may help to explain its therapeutic potential in the treatment of conditions characterized by chronic inflammation and increased oxidative stress.

scholarly journals Antioxidant Effects of Ocimum Gratissimum Leaf Essential Oils as a Supplement to Extender on Chilled Canine Sperm Quality

2020 ◽  
Author(s):  
Vui Van Nguyen ◽  
Samorn Ponchunchoovong ◽  
Sajeera Kupittayanant ◽  
Pakanit Kupittayanant
Keyword(s):  
Essential Oils ◽  
Sperm Quality ◽  
Antioxidant Properties ◽  
Quality Parameters ◽  
Control Group ◽  
Dependent Manner ◽  
Ocimum Gratissimum ◽  
Significant Difference ◽  
Antioxidant Effects

Abstract Background:Oxidative stress during chilled storage is a major problem withcanine sperm. To improve the quality of chilled canine sperm during storage, many synthetic antioxidants have been examined, but different outcomes were investigated depending on antioxidant properties. The bioactive compounds of essential oils fromOcimum gratissimumleaves are known as a natural antioxidant source. This study aimed to evaluate the antioxidant effects of essential oils from Ocimum gratissimumleavesas a supplement in extender on chilled canine sperm during 12 days of storage. Results:The results showed thatlow concentrations of Ocimum gratissimum essential oils (25, 50, and 100µg/mL) have beneficial effectson sperm quality, whereasOcimum gratissimumessential oils athigh levels (above 200µg/mL) have harmful effects. Specifically, the addition of 100µg/mL ofOcimum gratissimum essential oilsto the extender had the greatestbeneficial effect in improving the quality of chilled canine sperm, and had a significant difference in all sperm quality parameters except motility when compared to the control group (p<0.05). Conclusions:Ocimum gratissimum essential oilshave an impact on chilled canine sperm quality in a dose-dependent manner, and the best results areachieved with a maximum dose ofOcimum gratissimum essential oils of 100µg/mL.

scholarly journals Tetrahexyldecyl Ascorbate (THDC) Degrades Rapidly under Oxidative Stress but Can Be Stabilized by Acetyl Zingerone to Enhance Collagen Production and Antioxidant Effects

2021 ◽  
Vol 22 (16) ◽  
pp. 8756
Author(s):  
William R. Swindell ◽  
Manpreet Randhawa ◽  
Geovani Quijas ◽  
Krzysztof Bojanowski ◽  
Ratan K. Chaudhuri
Keyword(s):  
Oxidative Stress ◽  
Ascorbic Acid ◽  
Dermal Fibroblasts ◽  
Keratinocyte Differentiation ◽  
Full Potential ◽  
Type I ◽  
Interferon Signaling ◽  
Expression Of Genes ◽  
Improved Stability ◽  
The Stability

Tetrahexyldecyl Ascorbate (THDC) is an L-ascorbic acid precursor with improved stability and ability to penetrate the epidermis. The stability and transdermal penetration of THDC, however, may be compromised by the oxidant-rich environment of human skin. In this study, we show that THDC is a poor antioxidant that degrades rapidly when exposed to singlet oxygen. This degradation, however, was prevented by combination with acetyl zingerone (AZ) as a stabilizing antioxidant. As a standalone ingredient, THDC led to unexpected activation of type I interferon signaling, but this pro-inflammatory effect was blunted in the presence of AZ. Moreover, the combination of THDC and AZ increased expression of genes associated with phospholipid homeostasis and keratinocyte differentiation, along with repression of MMP1 and MMP7 expression, inhibition of MMP enzyme activity, and increased production of collagen proteins by dermal fibroblasts. Lastly, whereas THDC alone reduced viability of keratinocytes exposed to oxidative stress, this effect was completely abrogated by the addition of AZ to THDC. These results show that AZ is an effective antioxidant stabilizer of THDC and that combination of these products may improve ascorbic acid delivery. This provides a step towards reaching the full potential of ascorbate as an active ingredient in topical preparations.

scholarly journals Comparison Profile of Nutrition Status in Stage V Chronic Kidney Disease Patients Hemodialyzing Using New and Reuse Dialyzer

2019 ◽  
Vol 5 (2) ◽  
pp. 17
Author(s):  
Yuli Hermansyah ◽  
Ika Aulia Kurniasari ◽  
Heni Fatmawati
Keyword(s):  
Oxidative Stress ◽  
Wound Healing ◽  
Antioxidant Properties ◽  
Negative Control ◽  
Silver Sulfadiazine ◽  
Control Group ◽  
Burn Wound ◽  
Burn Wound Healing ◽  
Degree Burn ◽  
Significant Difference

Burn wound healing process consists of three phases of inflammation, proliferation and remodelling. The number of fibroblasts has increased during the proliferation phase. Oxidative stress in burn may disrupt proliferation through TGF-β inhibition, result in decreased number of fibroblasts and poor burn wound healing. Genistein in edamame seeds has anti-inflammatory, antibacterial and antioxidant properties. Edamame contains vitamins A, C, and E which is also have antioxidant properties. Edamame has been suggested to protect burn wound healing from oxidative stress. This study aimed to understand the effectiveness of edamame seed (Glycine max L. Merril) ethanolic extract (ED) to fibroblast count in second degree burn wound healing. This is true experimental research with post test only control group design. The sample of this research was 24 rat models of second degree burn which was made by attaching hot metal (60 ° C) for 5 seconds on the shaved back of the rats. The sample was divided into 6 groups with topical treatment, among others: positive control group (silver sulfadiazine), negative control group (Na CMC 0.5%), group with ED 20%, ED 40%, ED 60%, and ED 80%. Treatment was given for 15 days and skin sample was taken on the 16th day. Observation of the number of fibroblasts was done by taking pictures of histhologic preparations in Hematoxylin and Eosin staining under 400x magnification of light microscope. In one way ANOVA test, significance value of 0.011 (p≤0,05) indicated that there was at least a significant difference between 2 groups. LSD’s post hoc analysis showed that there was a significant difference in the group ED 20, 40, and 60% compared with negative control. Keyword: burn wound healing, vegetable soybean, genistein, fibroblast count, silver sulfadiazine

Antibacterial Properties and Application of Nanosilver Material in the Tracheal Tube

2020 ◽  
Vol 20 (10) ◽  
pp. 6542-6546
Author(s):  
Yunjie Hu ◽  
Xiaobei Ji ◽  
Dunshuang Wei ◽  
Jun Deng
Keyword(s):  
Endotracheal Tube ◽  
Endotracheal Intubation ◽  
Tracheal Tube ◽  
Antibacterial Properties ◽  
Plate Count ◽  
Control Group ◽  
Significant Difference ◽  
New Type ◽  
Experimental Group

To investigate the antibacterial ability of a new type of antibacterial tracheal tube coated with nanosilver/polyurethane in rats. In January 2016, 48 male SD rats of SPF grade, provided by the medical center of Hong Kong University of science and technology, Peking University, Shenzhen, were selected as the study objects. Twenty-four healthy rats, who underwent endotracheal intubation and retained nanosilver/polyurethane-coated new antibacterial endotracheal tube in vivo, were randomly selected as the experimental group, while 24 healthy rats who underwent endotracheal intubation at the same time and retained common endotracheal tube in vivo were randomly selected as the control group. At 12, 24, 48, and 72 hours after the operation, the number of colonies in the alveolar lavage fluid of the two groups was measured using the plate count method, and the thickness of the biofilm formed by the built-in catheter of the two groups was observed by microscope. Twelve hours after operation, there was no significant difference between the two groups (P <0.05). The colony number in BALF in the experimental group was significantly lower than that in the control group (P < 0.05). At 12 and 24 hours after operation, there was no significant difference in the biofilm thickness between the two groups (P > 0.05). In the experimental group, the thickness of biofilm that had formed by catheterization 48 and 72 hours after operation was significantly lower than that in the control group (P < 0.05). The new type of antibacterial tracheal tube, coated with nanosilver/polyurethane, has stronger antibacterial and anti-biofilm proliferation performance than that of the common tracheal tube.

The association between irisin levels, element distribution and oxidative stress markers in adolescent swimmers

2018 ◽  
Vol 0 (0) ◽  
Author(s):  
Muaz Belviranli ◽  
Nilsel Okudan ◽  
Nuran Karakuyu
Keyword(s):  
Oxidative Stress ◽  
Exercise Training ◽  
Venous Blood ◽  
Element Distribution ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
Significant Difference ◽  
And Gender ◽  
Antioxidant Effects ◽  
And Oxidative Stress

Abstract The objective of this investigation was to compare irisin concentration and its relation with oxidative stress markers, antioxidant enzymes and element levels in both male adolescent swimmers and sedentary controls. Twelve male swimmers aged between 11 and 18 years, who performed regular swimming training for at least 2 years and 12 age- and gender-matched sedentary controls participated in this study. After anthropometric measurements were performed, fasting venous blood samples were taken at rest. Irisin, malondialdehyde (MDA) as a marker of oxidative stress, superoxide dismutase (SOD) as a marker of antioxidant enzyme and elements (Zn, Cu, Se, Cr, Ni, Al, Fe) were analyzed in these samples. MDA levels were lower but Zn levels and Zn/Cu ratio were higher in the swimmers than in sedentary controls (p < 0.05). There was no statistically significant difference in the irisin levels and SOD activities between the groups (p > 0.05). The results of the current study suggested that exercise training has antioxidant effects and may reduce oxidative damage. Exercise training has also limited effects on irisin levels in the adolescents.

scholarly journals In Vitro Antioxidant Properties of Dichloromethanolic Leaf Extract of Gnidia glauca (Fresen) as a Promising Antiobesity Drug

2019 ◽  
Vol 24 ◽  
pp. 2515690X1988325 ◽  
Author(s):  
Wycliffe Arika ◽  
Cromwell Mwiti Kibiti ◽  
Joan Murugi Njagi ◽  
Mathew Piero Ngugi
Keyword(s):  
Oxidative Stress ◽  
Synergistic Effects ◽  
Antioxidant Properties ◽  
Iron Chelation ◽  
Reducing Power ◽  
Phytochemical Analysis ◽  
Traditional Practitioners ◽  
Antioxidant Effects ◽  
Hydroxyl Hydrogen

The acquisition of ethnobotanical information from traditional practitioners remains an empirical aspect of understanding the ethnopharmacology research. However, integration of information on chemical composition of plant extracts and their pharmacological activities forms a key resource for synthesis of new and effective therapeutics. In traditional African medicine, Gnidia glauca has folkloric remedies against obesity and its associated oxidative stress-mediated complications. However, the upsurge in its use has not been accompanied with scientific validations to support these claims. The present study aimed to determine the antioxidant potential of G glauca as a promising antiobesity agent. The antioxidant effects of the extract were assessed against 1,1-diphenyl-2-picrylhydrazyl, hydroxyl, hydrogen peroxide, nitric oxide, and superoxide radicals as well as lipid peroxidation, iron-chelating effect, and ferric-reducing power. Phytochemical analysis was conducted using gas chromatography linked to mass spectrophotometry. The results revealed that G glauca exhibited scavenging activities against all radicals formed. Besides, the extract showed iron chelation and ferric reducing abilities. The extract indicated a lower half maximal inhibitory concentration value than the standards used. For instance, the extract inhibited 50% of the formation of 2,2-diphenyl-1-picrylhydrazine at the concentration of 1.33 ± 0.03 mg/mL relative to 1.39 ± 0.06 mg/mL of the standard, vitamin C at 1% confidence limit. Similarly, the extract scavenged 50% of hydroxyl radical at 204.34 ± 10.64 μg/mL relative to 210.05 ± 8.80 μg/mL of gallic acid. The extract also contained various phytochemicals that have been associated with antiobesity effects. The synergistic effects of these phytocompounds increase their bioavailability and action on multiple molecular targets thereby correcting obesity-induced oxidative stress.

Impairment of the antioxidant properties of serum albumin in patients with diabetes: protective effects of metformin

2008 ◽  
Vol 114 (3) ◽  
pp. 251-256 ◽  
Author(s):  
Patrice Faure ◽  
Nicolas Wiernsperger ◽  
Camille Polge ◽  
Alain Favier ◽  
Serge Halimi
Keyword(s):  
Oxidative Stress ◽  
Fluorescence Quenching ◽  
Serum Albumin ◽  
Structural Changes ◽  
Antioxidant Properties ◽  
Protective Effects ◽  
Control Subjects ◽  
Significant Difference ◽  
Patients With Diabetes ◽  
Healthy Control

Free radical production is increased during diabetes. Serum albumin is a major antioxidant agent, and structural modification of albumin induced by glucose or free radicals impairs its antioxidant properties. Therefore the aim of the present study was to compare the antioxidant capacities and structural changes in albumin in patients with T2DM (Type 2 diabetes mellitus) treated with MET (metformin) or SU (sulfonylureas) and in healthy control subjects. Structural changes in albumin were studied by fluorescence quenching in the presence of acrylamide. Albumin thiols and fructosamines, reflecting oxidized and glycation-induced changes in serum albumin respectively, were assessed. Structural changes in albumin were demonstrated by a significant decrease in fluorescence quenching in patients with T2DM, with patients treated with MET exhibiting a significant difference in the conformation of albumin compared with patients treated with SU. Oxidation, resulting in a significant decrease in thiol groups and plasma total antioxidant capacity, and glycation, associated with a significant increase in fructosamines, were both found when comparing healthy control subjects with patients with T2DM. When patients treated with MET were compared with those treated with SU, oxidative stress and glycation were found to be significantly lower in MET-treated patients. In conclusion, patients with T2DM have a decrease in the antioxidant properties of serum albumin which may aggravate oxidative stress and, thus, contribute to vascular and metabolic morbidities. Moreover, a significant protection of albumin was found in patients with T2DM treated with MET.

scholarly journals Lipid Nanoparticles Containing Mixtures of Antioxidants to Improve Skin Care and Cancer Prevention

Pharmaceutics ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 2042
Author(s):  
Catarina Gonçalves ◽  
Maria João Ramalho ◽  
Renata Silva ◽  
Vera Silva ◽  
Rita Marques-Oliveira ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cancer Prevention ◽  
Antioxidant Properties ◽  
In Vitro Release ◽  
Human Keratinocytes ◽  
Natural Compounds ◽  
Skin Care ◽  
Tem Analysis ◽  
Antioxidant Effects

Oxidative stress, triggered by UV radiation, is one of the major causes of free radical-associated disorders, such as skin cancer. The application of natural compounds (NCs) with antioxidant effects can attenuate free radicals’ accumulation and, therefore, provide a strategy for skin care and cancer prevention. In this work, three natural compounds, naringenin, nordihydroguaiaretic acid (NDGA), and kaempferol, were encapsulated into nanostructured lipid carriers (NLCs) aiming for the development of a formulation for cutaneous application with antioxidant properties. For the experiments, different formulation parameters were evaluated to optimize the NLCs that showed a diameter around 200 nm, which is an adequate particle size for incorporation in cosmetics. Transmission electron microscopy (TEM) analysis confirmed the NLCs’ typical spherical morphology. Encapsulation efficiency (EE) and loading capacity (LC) values revealed an effective production process, with EEs over 90% and LCs near the maximum value. The developed NLCs revealed a prolonged in vitro release of the natural compounds. The NLCs were stable under storage conditions, maintaining their psychochemical characteristics for 30 days. Additionally, they did not show any physical instability in accelerated stability studies, which also suggests long-term stability. Finally, the NCs antioxidant activity was evaluated. Interestingly, the NDGA and kaempferol mixture provided an antioxidant synergic effect. The NLC formulations’ cytotoxicity was tested in vitro in immortalized human keratinocytes (HaCaT). In addition, putative antioxidant effects of the developed NLC formulations against tert-butyl hydroperoxide (t-BHP)-induced oxidative stress were studied, and the NDGA-loaded NLC was revealed to be the one with the most protective effect. Therefore, we concluded that the naringenin, NDGA, and kaempferol incorporation into NLCs constitutes a promising strategy to increase their bioavailability and delivery to the skin.

scholarly journals Pulmonary Protective Effects of Propofol on Cisplatin-Induced Oxidative Damage in Male Rats

2020 ◽  
Vol 1 (2) ◽  
pp. 72-75
Author(s):  
Sajjad Makhdoomi ◽  
Akram Oftade ◽  
Sodabe Khodabandehlou ◽  
Akram Ranjbar
Keyword(s):  
Oxidative Stress ◽  
Lung Tissue ◽  
Antioxidant Properties ◽  
Male Rats ◽  
Protective Effects ◽  
Protected Group ◽  
Significant Difference ◽  
Total Antioxidant ◽  
Pulmonary Damage ◽  
Male Wistar Rats

Background: The present study was performed to investigate the protective effects of propofol against cisplatin-induced pulmonary toxicity in rats. Methods: A total of 20 male Wistar rats weighing 180-250 g were divided into four groups of control, the cisplatin-intoxicated group intraperitoneally (IP) injected with cisplatin (7 mg/kg/d for a week), the propofol group (10 mg/kg/d, IP), and the protected group receiving propofol (10 mg/kg/d, IP) poisoned by cisplatin. Then, the biomarkers of total antioxidant capacity (TAC), catalase (CAT) activity, and lipid peroxidation (LPO) were measured in homogeneous lung tissues. Results: The data revealed the evidence of oxidative stress in the lung tissue of cisplatin-intoxicated rats as indicated by an increase in the level of LPO compared with propofol and protected groups (P<0.05). Moreover, TAC decreased in the cisplatin group while it increased in the propofol group compared to cisplatin and protected groups (P<0.05). No significant difference was observed between the groups regarding CAT (P>0.05). Protection with propofol ameliorated the oxidative stress induced by cisplatin in the lung tissue because of the reduction of LPO. Conclusion: According to these results, it seems that propofol provides a remarkable protection against cisplatin-induced oxidative pulmonary damage mediated by its antioxidant properties.

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