Productivity Improvement of Faba Bean (Vicia faba L.) through Elite Rhizobial Inoculants in the Central Highlands of Ethiopia

This study envisioned to assess the inoculation response of faba bean to six indigenous rhizobial isolates, 100 kg ha-1 DAP as a positive control and non-inoculated negative control under farmers’ field conditions at Welmera district, Ethiopia. The results revealed the presence of significantly different treatments (p ≤ 0.05) in grain yield. The highest grain yields (3966 kg ha-1and 3694 kg ha-1) were obtained from inoculation of rhizobial isolates (FB-AR-13 and FB-1018) during the 2019/20 and 2020/21 cropping seasons. Based on the two successive year’s average grain yield response, FB-AR-13 (3966 kg ha-1) became the first most performing isolate. The partial budget analysis results also showed that FB-AR-13 was the most promising isolate that showed a higher MRR 6422 %, which is 43% higher than the MRR of the local standard check FB-1018. Therefore, isolate FB-AR-13 is the best promising candidate for the development of commercial faba bean rhizobial inoculant in the central high lands of Ethiopia after further verification over different farmers' fields at different agro-ecologies.

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Performance of Large Seeded Faba Bean Genotypes for Yield and Related Agronomic Traits in Different Parts of Ethiopia

East African Journal of Agriculture and Biotechnology ◽

10.37284/eajab.4.1.486 ◽

2021 ◽

Vol 4 (1) ◽

pp. 1-11

Author(s):

Mesfin Tadele ◽

Mussa Jarso ◽

Sisay Argaye ◽

Asnakech Tekalign ◽

Nigat Tilahun ◽

...

Keyword(s):

Disease Resistance ◽

Grain Yield ◽

Seed Size ◽

Faba Bean ◽

Agronomic Traits ◽

Block Design ◽

Commercial Production ◽

Breeding Lines ◽

Rust Diseases ◽

Cropping Seasons

Faba bean (Vicia faba L.) is a high value crop in Ethiopia and has versatile uses. The national faba bean breeding program concentrates on the three major traits (grain yield, disease resistance and seed size) of the crop for varietal release for commercial production. Hence, ten faba bean genotypes were evaluated at Adet, Areka, Bekoji, Haramaya, Holetta and Jimma during the main cropping seasons of 2018 and 2019 using a randomized complete block design with four replications with the objectives to select the genotype with best performance in terms of important agronomic traits like grain yield, disease resistance, large seed size and other desirable agronomic traits for high potential production areas in Ethiopia. The combined analysis of variance across locations revealed that there is highly significant (P < 0.01) variation among the tested genotypes for grain yield, 1000-seeds weight, number of pods per plant, and days to 90% physiological maturity. The genotype EH011089-3 showed better performance than the tested genotypes having comparable grain yield performance (3803 kgha-1) with the two standard checks, Numan and Gora (3790 and 3897 kgha-1, respectively) while it had the highest 1000-seeds weight (1065 g) compared to the two standard checks, Numan and Gora (937 g and 786 g, respectively), i.e., 13.7% and 35.5% advantage, over the two standard checks, respectively. Additionally, EH011089-3 had better resistance for chocolate spot and rust diseases. Therefore, EH011089-3 was the best over the tested varieties and breeding lines. Genotype EH011089-3 is recommended for varietal release for commercial production all over in Ethiopia.

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Fungicide Management of Faba Bean Gall (Olpidium viciae) in Ethiopia

Turkish Journal of Agriculture - Food Science and Technology ◽

10.24925/turjaf.v7i7.1075-1081.2189 ◽

2019 ◽

Vol 7 (7) ◽

pp. 1075 ◽

Cited By ~ 2

Author(s):

Wulita Wondwosen Kebede ◽

Mashilla Dejene W/Michael ◽

Negussie Tadesse ◽

Seid Ahmed Kemal

Keyword(s):

Grain Yield ◽

Faba Bean ◽

Natural Infection ◽

Block Design ◽

Severity Index ◽

Northern Ethiopia ◽

Disease Progress Curve ◽

Disease Progress ◽

Budget Analysis ◽

Progress Curve

Various biotic, abiotic and socio-economic factors negatively affect the productivity of the crop. Among these, a new disease known as Faba bean galls (Olpidium viciae) has become a serious threat to faba bean production and productivity in highland areas of central and northern Ethiopia. Thus, field experiment was conducted in Lay Gorebela and Mush to assess the efficacies of fungicides for the management of faba bean gall under natural infection. Six fungicides were evaluated alongside control in randomized complete block design in three replications. Fungicides showed different levels of efficiency at both locations in both seasons. In 2014/15, the highest percent severity index and area under disease progress curve were calculated from control plots at both locations. At Lay Gorebela, higher grain yields were recorded from Metalaxyl 8% + Mancozeb 64% WP and Triadimefon 250 g/L sprayed plots whereas plots sprayed with Metalaxyl 8% + Mancozeb 64% WP gave higher yield at Mush. Similar trends in percent severity index and grain yield were observed with control treatment in 2015/16. Percent severity index and area under disease progress curve were negatively correlated with grain yield and positively correlated with each other at both locations and seasons. Partial budget analysis indicated as Triadimefon 250 g/L and Metalaxyl 8% + Mancozeb 64% WP were cost-effective. The chemical treatment could be used as a short-term control strategy, and a component in integrated management of faba bean galls.

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Non-Chemical Control of Charcoal Rot of Urdbean by Sonchus oleraceous Application

Planta Daninha ◽

10.1590/s0100-83582020380100044 ◽

2020 ◽

Vol 38 ◽

Author(s):

S. BANARAS ◽

A. JAVAID ◽

A. SHOAIB

Keyword(s):

Grain Yield ◽

Sandy Loam ◽

Dry Weight ◽

Positive Control ◽

Negative Control ◽

Growth And Yield ◽

Charcoal Rot ◽

Shoot Dry Weight ◽

Dry Biomass ◽

Different Doses

ABSTRACT: Urdbean [Vigna mungo (L.) Hepper] is an important leguminous crop whose production is severely affected by charcoal rot disease caused by Macrophomina phaseolina. This study was undertaken to seek an environmental friendly alternative to synthetic fungicides for management of this disease. Dry biomass of an allelopathic weed Sonchus oleraceous L. was used to combat the menace. Fumigated sandy loam pot soil, pre-inoculated with M. phaseolina, was amended with different doses of dry biomass of the weed ranging from 0.5% to 3%. Application of 2% weed biomass completely controlled the disease. Soil inoculation with M. phaseolina inoculation (positive control) reduced shoot dry weight and grain yield of urdbean by 59% and 91%, respectively, over negative control. Application of different doses of soil amendment in pathogen inoculated soil enhanced shoot dry weight and grain yield of urdbean by 107-307% and 438-7400%, respectively, over positive control. The highest positive effect on growth and yield of uedbean in M. phaseolina contaminated soil was recorded due to 2.5% amendment. M. phaseolina inoculation significantly enhanced peroxidase (POX) and phenyl alanine ammonia lyase (PAL) activities. However, application of different doses of S. oleraceous biomass to the soil gradually decreased activities of these enzymes. The present study concludes that application of 2.5% dry biomass of S. oleraceous can completely control charcoal rot of urdbean and significantly enhance crop growth and yield.

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Evaluation of Different Level of Vermicompost on Yield and Yield Components of Wheat at Vertisols of L/Machew District

Asian Soil Research Journal ◽

10.9734/asrj/2020/v4i230089 ◽

2020 ◽

pp. 21-27

Author(s):

Weldegebriel Gebrehiwot ◽

Tewolde Berhe ◽

Tsadik Tadele ◽

Kinfe Tekulu ◽

Geberesemaeti Kahsu ◽

...

Keyword(s):

Grain Yield ◽

Yield Components ◽

Phosphate Fertilizer ◽

Fertilizer Application ◽

Marginal Rate ◽

Total N ◽

Budget Analysis ◽

Yield And Yield Components ◽

Marginal Rate Of Return ◽

Cropping Seasons

A field experiment was carried out to evaluate different rates of vermicompost, and inorganic NPS(Nitrogen-Phosphate Fertilizer With Sulphur) fertilizers for wheat production in 2015-2017 main cropping seasons at L/machew district of the Tigray Regional State. There were five systematically combined rates of vermicompost and one rate of NPS treatments during the study. The experimental design was randomized complete block with three replications. Surface soil samples were collected before planting to analyze selected soil properties. The textural class of the soil was clay. The pH of the soil was rated under moderately acidic. Available P was rated under medium in most of the experimental sites while total N was low in all experimental sites. The treatments significantly affected crop phenology, yield and yield components of wheat. Thus tallest plant height was found from treatments that received 100kg/ha NPS, followed by 8.75t/ha vermicompost which resulted in (73.25cm) while the shortest plant (62.73cm) was obtained from control plots, respectively. Highest mean wheat grain yield (2642.8kg/ha) was obtained in response to 100kg NPS from inorganic fertilizer application followed by the second highest dose of vermicompost (7t/ha) with grain yield of 2620 kg/ha. At the wheat plants grown in plot received vermicompost compost at 7t/ha of vermicompost increased the grain yield by 67.78% over the control. Though highest grain yield was obtained from highest dose of vermicompost the partial budget analysis shows that the highest marginal rate of return (456) was obtained from 3.5t/ha VC ha-1 plus 100kg/ha urea at the study area.

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Lowland Rice Yield as Affected by Straw Incorporation and Inorganic Fertilizer Over Cropping Seasons in Fluvisol

JOURNAL OF ADVANCES IN AGRICULTURE ◽

10.24297/jaa.v3i1.5409 ◽

2014 ◽

Vol 3 (1) ◽

pp. 129-141

Author(s):

Brahima Koné ◽

Zadi Florent ◽

Gala bi Trazié Jeremie ◽

Akassimadou Edja Fulgence ◽

Konan Kouamé Firmin ◽

...

Keyword(s):

Grain Yield ◽

Rice Variety ◽

Mineral Fertilizer ◽

Lowland Rice ◽

Inorganic Fertilizer ◽

Cropping Season ◽

Significant Difference ◽

Straw Incorporation ◽

Cropping Seasons ◽

Fertilizer Effect

Grain yield stabilization of lowland rice over cropping seasons was explored using different compositions of inorganic fertilizers (NPK, NPKCa, NPKMg, NPKZn, NPKCaMg, NPKCaZn and NPKCaMgZn) and straw incorporation (3, 6, 9, 12 and 15 tha-1 ). No fertilizer and no straw amended plot was the control in a split-plot design with three replications laid in a Fluvisol of Guinea savanna in Centre Cote d’Ivoire. Three weeks old nursery rice variety NERICA L19 was transplanted. No significant difference of grain yield was observed between the different treatments excluding the highest yields recorded for treatments NPKMg (5.09 tha-1 ), NPKZn (5.15 tha-1 ) and NPKCaéMg (5.31 tha-1 ) compared with 12 (3.95 tha1 ) and 15 tha-1 (4.14 tha-1 ) as straw rates respectively. Grain yield declining trend was more pronounced for mineral fertilizer treatments showing twice greater depressive effect of cropping cycle compared with the straw especially, for treatments characterized by highest grain yield in the first cropping season and similar grain yields were recorded for both sources of nutrient in the third cropping cycle. Of slowness of nutrients releasing by straw, highest grain yield was expected for this soil amender within a longer period of cultivation whereas, unbalance soil micronutrients should be relevant to studious declining yield under inorganic fertilizer effect. Nevertheless, the straw rate of 12 tha-1 supplying 0.58% of NPK as mineral fertilizer equivalent can be recommended for sustaining lowland rice production in the studied agro-ecosystems unless for three cropping seasons.

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Aktivitas Antifungi Air Perasan Bawang Merah (Allium ascalonicum L.) Terhadap Candida albicans Secara In Vitro

Jurnal Ilmu Kedokteran ◽

10.26891/jik.v9i2.2015.71-77 ◽

2017 ◽

Vol 9 (2) ◽

pp. 71

Author(s):

Nurhasanah Nurhasanah ◽

Fauzia Andrini ◽

Yulis Hamidy

Keyword(s):

Candida Albicans ◽

Antifungal Activity ◽

Allium Sativum ◽

Fungicidal Activity ◽

Positive Control ◽

Negative Control ◽

Randomized Design ◽

Significant Difference ◽

Completely Randomized Design

Shallot (Allium ascalonicum L.) has been known as traditional medicine. Shallot which has same genus with garlic(Allium sativum L.) contains allicin that is also found in garlic and has been suspected has fungicidal activity toCandida albicans. It is supported by several researches. Therefore, shallot is suspected has antifungal activity too.The aim of this research was to know antifungal activity of shallot’s water extortion againsts Candida albicans invitro. This was a laboratory experimental research which used completely randomized design, with diffusion method.Shallot’s water extortion was devided into three concentrations, there were 50%, 100% and 200%. Ketoconazole 2%was positive control and aquadest was negative control. The result of this research based on analysis of varians(Anova), there was significant difference between several treatments and was confirmed with Duncan New MultipleRange Test (DNMRT) p<0,05, there was significant difference between 100% shallot’s water extortion with othertreatments, but there was no significant difference between 50% shallot’s water extortion with 200% shallot’s. Theconclusion was shallot’s water extortion had antifungal activity againsts Candida albicans with the best concentration100%, but it was lower than ketoconazole 2%.

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The Comparison of Ostecyte in the Pressure area and Tension area on Tooth Movement Because of Hyperbaric Oxygen Therapy

DENTA ◽

10.30649/denta.v12i1.162 ◽

2018 ◽

Vol 12 (1) ◽

pp. 34

Author(s):

Arya Barahmanta ◽

Muhammad Faizal Winaris ◽

Pambudi Raharjo

Keyword(s):

Hyperbaric Oxygen ◽

Oxygen Therapy ◽

Tooth Movement ◽

Bone Remodelling ◽

Orthodontic Tooth Movement ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Pressure Area

Background: Orthodontic tooth movement is a interaction prosess of resorption and deposition of bone remodeling. Orthodontic tooth movement by mechanical strength causes changes in alveolar bone. Osteocyte is an essential cell to respond bone remodelling. Hyperbaric Oxygen Therapy affects production of osteocyte because it can release Reactive Oxygen Species (ROS) and Nitrid Oxide (NO). Purpose: To determine the difference number of osteocyte in pressure and tension area during tooth movement by adjuvant of Hyperbaric Oxygen 2,4 ATA during 7 days starting on day 8 to day 14. Materials and Methods: This research used Completery Randomized Control Group Post Test Only Design. 36 cavia cobaya (male) were divided into 3 groups randomly : the negative control groups, positive control group, and treatment group. Preparat staining used Hematoxylin Eosin (HE) and calculated on microscop 1000x with 20 field of view. Data analyses used one way ANOVA and LSD test then compared each area by using paired T test. Result: The data showed that the treatment group (P=10,67) tension area has the highest number of osteocyte than negative control group (K-=3,67), positive control (K+=7,42). In the pressure area showed that negative control group (K-=5,00) has the highest than positive control group (K+=3,83) and treatment (P=3,25). Conclusion: Therapy HBO 2,4 ATA 7 days starting on day 8 to day 14 is could increase osteocyte in the tissue to stimulate process of bone remodelling.<pre> </pre>Keywords: Hyperbaric Oxygen, Tooth movement, Bone remodeling, Osteocyte Correspondence: Arya Brahmanta, Department of Orthodonty, Faculty of Dentistry, Hang Tuah University, Arif Rahman Hakim 150, Surabaya, Phone 031-5945864, Email: <a href="mailto:[email protected]">arya.brahmanta@hangtuah.ac.id</a>

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UJI AKTIVITAS HEPATOPROTEKTOR KOMBINASI EKSTRAK ETANOL DAUN PANDAN WANGI (Pandanus amaryllifolius roxb) DAN KAYU MANIS (Cinnamomum burmanii) TERHADAP KADAR MDA YANG DIINDUKSI PARASETAMOL

Jurnal Kesehatan Kusuma Husada ◽

10.34035/jk.v12i1.580 ◽

2020 ◽

pp. 68-73

Author(s):

Yuni Asri Mulatsih Agami ◽

Eka Wisnu Kusuma

Keyword(s):

Oxidative Stress ◽

Ethanol Extract ◽

Optimal Dose ◽

Experimental Methods ◽

Positive Control ◽

Negative Control ◽

Male Rats ◽

Negative Group ◽

Ic50 Value ◽

Dose Combination

Kasus penyakit hati semakin meningkat seiring penggunaan senyawa hepatotoksin salah satunya karena penggunaan parasetamol dengan dosis berlebih. Hal tersebut dapat meningkatkan produksi radikal bebas sehingga memicu terjadinya stress oksidatif yang dapat menimbulkan kerusakan jaringan yang ditandai dengan peningkatan kadar Malondialdehyde (MDA). Stress oksidatif dapat diatasi dengan antioksidan dari berbagai tanaman. Kulit kayu manis memiliki aktivitas antioksidan dengan nilai IC50 53ppm dan daun pandan wangi 39,7% Penelitian ini bertujuan untuk mengetahui aktivitas kombinasi ekstrak etanol daun pandan wangi dan kayu manis dalam menurunkan kadar MDA. tikus yang diinduksi parasetamol. Penelitian menggunakan metode eksperimental, dilakukan selama 9 hari dengan 30 ekor tikus jantan dibagi menjadi 6 Kelompok, yaitu: Normal diberi aquadest, Kontrol Positif diberi silimarin 100 mg/kgBB, Kontrol Negatif diberi CMC-Na 0,05%, serta 3 kelompok lainnya diberi kombinasi ekstrak daun pandan wangi:kayu manis berturut-turut dosis I (25:75), dosis II (50:50), dosis III (75:25). Semua kelompok diinduksi parasetamol 2,5 g/kgBB pada hari ke-7 setelah 30 menit perlakuan, kecuali kelompok normal. Pada hari ke 9 dilakukan pengukuran kadar MDA dengan metode TBARs menggunakan spektrofotometri. Pemberian kombinasi ekstrak etanol daun pandan wangi dan kayu manis dapat menurunkan kadar MDA dengan kombinasi dosis yang paling optimal adalah 75:25 berdasarkan statistik dengan nilai signifikan 0,000<0,05 dibandingkan dengan kelompok negatif. Cases of liver disease have increased with the use of hepatotoxin compounds, one of which is due to the use of paracetamol with excessive doses. This can increase the production of free radicals so that it triggers oxidative stress which can cause tissue damage which is characterized by increased levels of Malondialdehyde (MDA). Oxidative stress can be overcome with antioxidants from various plants. Cinnamomum burmanii has antioxidant activity with IC50 value of 53ppm and Pandanus amarrylifolius 39.7%. This study aims to determine the combined activity of ethanol extract of Pandanus amarrylifolius and Cinnamomum burmanii in reducing MDA levels. Paracetamol-induced rats. Research using experimental methods, conducted for 9 days with 30 male rats divided into 6 groups, namely: Normal given aquadest, Positive Control were given silimarin 100 mg / kgBB, Negative Control was given CMC-Na 0.05%, and 3 other groups were given a combination of Pandanus amarrylifolius extract: Cinnamomum burmanii dose I (25:75), dose II (50:50), dose III (75:25). All groups induced paracetamol 2.5 g / kgBB on the 7th day after 30 minutes of treatment, except the normal group. On the 9th day MDA levels were measured using the TBARs method using spectrophotometry. Giving a combination of Pandanus amarrylifolius and Cinnamomum burmanii ethanol extract can reduce MDA levels with the most optimal dose combination is 75:25 based on statistics with a significant value of 0,000<0.05 compared with the negative group.

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UJI IMUNOMODULATOR DARI EKSTRAK ETANOL DAUN WARU (HIBISCUS TILIACEUS) DENGAN METODE HIPERSENSITIVITAS TIPE LAMBAT

Jurnal Penelitian Farmasi & Herbal ◽

10.36656/jpfh.v1i2.103 ◽

2019 ◽

Vol 1 (2) ◽

pp. 11-16

Author(s):

Yanna Rotua Sihombing ◽

Debi Dinha Sitepu

Keyword(s):

Treatment Group ◽

Leaf Extract ◽

Positive Control ◽

Negative Control ◽

Delayed Type Hypersensitivity ◽

Hypersensitivity Response ◽

Delayed Type Hypersensitivity Response

Immunomodulator is a compound that can increaase the imuno system. One of the plants that have immunomodulator’s activity is Waru Leaf (Hibiscus tiliaceus). the purpose of this research was to test the effect of immunomodulator by extract of Waru Leaf ethanol on rat male. The activity of immunomodulator was determined by using digital pletysmometer by measuring the differences between the last leg swelling’s volume and the first leg swelling’s volume. The treatment group were divided into 5 groups. Each group consistof 5 rats CMC-Na 0,5% (negative control), Stimuno® 32,5 mg/kgBW (positive control), dose of EEDW 50, 100 and 200 mg/kgBW, and bacteria E.coli as antigen. The results slowed that distribution of EEDW dose 200 mg/kgBW can give the effect of immunostimulant by swelling enthancement compared by CMC-Na 0,5 %. EEDW 200 mg/kgBW that have activity comparable with Stimuno® 32,5 mg/kgBW. Thus, it is concluded that of Waru Leaf extract has immunomodulator effects on delayed-type hypersensitivity response of rat male.

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In vitro and in vivo Assessment of Silver Nanoparticles Against Clostridium botulinum Type A Botulinum

Current Drug Discovery Technologies ◽

10.2174/1570163815666180403163946 ◽

2019 ◽

Vol 16 (1) ◽

pp. 113-119 ◽

Cited By ~ 1

Author(s):

Mohammad Aminianfar ◽

Siavash Parvardeh ◽

Mohsen Soleimani

Keyword(s):

Silver Nanoparticles ◽

Botulinum Toxin ◽

Clostridium Botulinum ◽

Lethal Dose ◽

Type A ◽

Positive Control ◽

Negative Control ◽

Laboratory Animals ◽

Control Group ◽

Nano Silver

Background: Clostridium botulinum causes botulism, a serious paralytic illness that results from the ingestion of a botulinum toxin. Because silver nanoparticle products exhibit strong antimicrobial activity, applications for silver nanoparticles in healthcare have expanded. Therefore, the objective of the current study was to assess a therapeutic strategy for the treatment of botulism toxicity using silver nanoparticles. Methods: A preliminary test was conducted using doses that produce illness in laboratory animals to determine the absolute lethal dose (LD100) of botulinum toxin type A (BoNT/A) in mice. Next, the test animals were divided into six groups containing six mice each. Groups I, II and III were the negative control (botulinum toxin only), positive control-1 (nano-silver only) and positive control-2 (no treatment), respectively. The remaining groups were allocated to the toxin that was supplemented with three nano-silver treatments. Results: The mortality rates of mice caused by BoNT/A significantly reduced in the treatment groups with different doses and injection intervals of nano-silver when compared to the negative control group. BoNT/A toxicity induced by intraperitoneal injection of the toxin of Clostridium botulinum causes rapid death while when coupled with nano-osilver results in delayed death in mice. Conclusion: These results, while open to future improvement, represent a preliminary step towards the satisfactory control of BoNT/A with the use of silver nanoparticles for human protection against this bioterrorism threat. Further study in this area can elucidate the underlying mechanism for detoxifying BoNT/A by silver nanoparticles.

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