scholarly journals Comparison of Protective Effect of Apium graveolens and Aloe vera Supplemented with Zinc on Cadmium Induced Hepato and Nephro-Toxicity in Wistar Rats

2021 ◽  
Vol 10 (28) ◽  
pp. 2083-2088
Author(s):  
Sushanth N.K. ◽  
Vijayaraghavan R. ◽  
Vijayalakshmi S ◽  
Senthilkumar Sivanesan ◽  
Madhan Kumar Swaminathan ◽  
...  
Keyword(s):  
Protective Effect ◽  
Wistar Rats ◽  
Antioxidant Properties ◽  
Aloe Vera ◽  
Environmental Pollutant ◽  
Apium Graveolens ◽  
Control Group ◽  
Albino Rats ◽  
Liver And Kidney ◽  
Wistar Albino Rats

BACKGROUND Cadmium (Cd) is an environmental pollutant that accumulates in various organs such as liver, kidney, and other organs. It generates reactive oxygen species, thereby resulting in pathological changes in the organs it accumulates in by depleting antioxidants. Apium graveolens (AG) and Aloe vera (AV) are rich sources of antioxidants. Zinc (Zn) is an important antioxidant trace element present in various tissues and this protects the organs from the toxic effects of cadmium. We wanted to compare the protective effect of AG and AV with and without Zn supplementation in Cd exposed liver and kidneys of Wistar rats. METHODS Male Wistar albino rats were divided into 11 groups. The control group received only vehicle, the experimental groups were administered with 10 mg / Kg bw of CdCl2, 40mg / Kg bw of ZnCl2, 200 mg / Kg bw of AG and AV, 400mg / K bw AG and AV separately and in combination. After 56 days, the animals were sacrificed and histopathology was done. RESULTS Cd resulted in significant tissue damage of liver and kidney. AG, AV and Zn were able to offer protection to these tissues. CONCLUSIONS AG, AV and Zn by virtue of their antioxidant properties, protect the liver and kidney from damages due to Cd more effectively in rats. KEY WORDS Cadmium, Zinc, Kidney, Liver, Apium graveolens, Aloe vera

scholarly journals Protective Effect of Hydroalcoholic Extract of Aloe Vera Supplemented by Zinc on the Testis of Wistar Albino Rats Exposed to Cadmium

2020 ◽  
Vol 11 (SPL4) ◽  
pp. 2128-2133
Author(s):  
Sushanth N K ◽  
Vijayalakshmi S ◽  
Dayanand Reddy G ◽  
Sunil Kumar K N ◽  
Shakila R
Keyword(s):  
Body Weight ◽  
Protective Effect ◽  
Environmental Pollutants ◽  
Aloe Vera ◽  
Reproductive Organs ◽  
Health Concern ◽  
Control Group ◽  
Albino Rats ◽  
Hydroalcoholic Extract ◽  
Wistar Albino Rats

Infertility is a major health concern that may be caused due to inadequate fertility hormones or poor quality of reproductive parameters. The environmental pollutants such as cadmium is one of the causative factors for inducing infertility in men. Cadmium accumulates in various tissues and affects reproductive organs, especially testis which is highly sensitive to cadmium poisoning. In this experiment, protective effect of hydroalcoholic extract (HAE) of Aloe vera with and without zinc has been evaluated. Zinc, protects the testes from damages resulting due to cadmium. Aloe vera is a very good source of antioxidants such as vitamin C and E. In this experiment male Wistar albino rats were grouped into 5. The control group received only the vehicle. Experimental groups were administered in Cd group with 10mg/Kg body weight of CdCl2, in Cd+Zn group 40mg/Kg body weight of CdCl2 and 40mg/Kg bodyweight of ZnCl2, in Cd+AV group 10mg/Kg body weight of CdCl2 and 200mg/Kg body weight of HAE of Aloe vera and in Cd+AV+Zn 10mg/Kg body weight of CdCl2, 200mg/Kg body weight of HAE of Aloe vera and 40mg/Kg bodyweight of ZnCl2. The results showed that cadmium caused extensive testicular damage which was protected by HAE of Aloe vera and zinc. Hence, protective effect of HAE of Aloe vera  was exhibited clearly in cadmium treated rats, however, supplementing Aloe vera with zinc provided better protection.

scholarly journals Effects of Safranal on Tissue Oxidative Stress in Sub-Chronic Thinner-Addicted Rats

2020 ◽  
Vol 71 (1) ◽  
pp. 1997
Author(s):  
M. DÜZ ◽  
A. F. FIDAN
Keyword(s):  
Oxidative Stress ◽  
Nitric Oxide ◽  
Protective Effect ◽  
Total Antioxidant Capacity ◽  
Reduced Glutathione ◽  
Control Group ◽  
Albino Rats ◽  
Total Antioxidant ◽  
Wistar Albino Rats ◽  
And Oxidative Stress

The present study was carried out to determine the effects of sub-chronic thinner addiction on the oxidant-antioxidant balance and oxidative stress on certain tissues and the possible protective effect of safranal against thinner toxication in rats. Adult male Wistar albino rats were randomly divided into four groups of 10 animals each as follows: control (C), safranal (S), thinner (T) and thinner+safranal (T+S). The control group received 1cc saline by gastric gavage. Safranal was administered to S and T+S groups by using gastric gavage at a dose of 100 mg/kg/day and volume of 0.1 mL/kg/day. Thinner inhalation was applied to T and T+S groups in a container with NaOH tablets twice a day. Levels of malondialdehyde (MDA), reduced glutathione (GSH), nitric oxide (NOx) metabolites, total antioxidant capacity (TAS) and total oxidant capacity (TOS) were determined in liver, lung, brain, kidney and testis tissues of the rats. In the T+S group, it was observed that the MDA levels significantly decreased in all tissues, except the kidney, in comparison to the thinner inhalation group (p = 0.000). When the NOx levels of the T+S group were compared with the levels of the T group, it was concluded that there existed a statistically significant decrease in the NOx levels in alltissues (p = 0.000). In T+S group, it was observed that safranal either eliminated or mitigated oxidative stress that developed in tissues through decreasing MDA and TOS levels and increasing GSH and TAS levels and caused significant decreases in NOX levels in all tissues. As a result, it was determined that safranal, although not uniform for all tissue types, had a protective potential against the damaging effects of oxidative stress caused by sub-chronic thinner inhalation.

scholarly journals Vitamin B17 Ameliorates Methotrexate-Induced Reproductive Toxicity, Oxidative Stress, and Testicular Injury in Male Rats

2020 ◽  
Vol 2020 ◽  
pp. 1-11 ◽  
Author(s):  
Shatha G. Felemban ◽  
Maha A. Aldubayan ◽  
Ahmad H. Alhowail ◽  
Ibtesam S. Almami
Keyword(s):  
Protective Effect ◽  
Antioxidant Properties ◽  
Reproductive Toxicity ◽  
Male Rats ◽  
Nuclear Antigen ◽  
Plasma Testosterone ◽  
Control Group ◽  
Albino Rats ◽  
Testicular Toxicity ◽  
Testicular Weight

Methotrexate (MTX; 4-amino-10-methylfolic acid) is a folic acid reductase inhibitor used to treat autoimmune diseases and certain types of cancer. Testicular toxicity resulting from MTX is a significant side effect that may cause subsequent infertility. The present study was conducted to examine the ameliorating effects of vitamin B17 (VitB17) against testicular toxicity induced by MTX in male rats. A total of 50 male albino rats were equally divided into five groups [control group; vitamin B17 group (VitB17) administered VitB17 only; methotrexate group administered MTX only; cotreated group, (VitB17+MTX) and posttreated group (MTX+VitB17)]. In methotrexate group (MTX), a significant decrease was observed in body weight and the testicular weight, as well as the levels of plasma testosterone, luteinizing hormone and follicle-stimulating hormone compared with control. The sperm count, viability, morphology index, total motility, and progressive motility also decreased in MTX rats compared with control. Furthermore, the levels of reduced glutathione, catalase, and superoxide dismutase, as well as proliferating cell nuclear antigen protein expression, in the testicular tissue decreased in MTX compared with control. In addition, MTX caused a significant increase in DNA and tissue damage compared with control. However, VitB17 ameliorated these effects, indicating that it has a preventative and curative effect against MTX-induced reproductive toxicity in male rats. The protective effect of VitB17 may be associated to its antioxidant properties as it possibly acts as a free-radical scavenger and lipid peroxidation inhibitor, as well as its protective effect on the levels of GSH, SOD, and CAT.

scholarly journals Comparative assessment on the probable mechanisms underlying the hepatorenal toxicity of commercial imidacloprid and hexaflumuron formulations in rats

2022 ◽  
Author(s):  
Eman I. Hassanen ◽  
Ahmed M. Hussien ◽  
Sally Mehanna ◽  
Marwa A. Ibrahim ◽  
Neven H. Hassan
Keyword(s):  
Kidney Tissue ◽  
Serum Levels ◽  
Saline Group ◽  
Control Group ◽  
Albino Rats ◽  
Apoptosis Pathway ◽  
Liver And Kidney ◽  
Group 2 ◽  
Wistar Albino Rats ◽  
Tissue Specimens

Abstract Pesticides are viewed as a major wellspring of ecological contamination and causing serious risky consequences for people and animals. Imidacloprid (IM) and hexaflumuron (HFM) are extensively utilized insect poisons for crop assurance on the planet. A few investigations examined IM harmfulness in rodents, but its exact mechanism hasn’t been mentioned previously as well as the toxicity of HFM doesn’t elucidate yet. For this reason, the present study was designed to explore the mechanism of each IM and HFM–evoked rat liver and kidney toxicity and to understand its molecular mechanism. 21 male Wistar albino rats were divided into 3 groups, as follows: group (1), normal saline; group (2), IM; and group (3), HFM. Both insecticides were orally administered every day for 28 days at a dose equal to 1/10 LD50 from the active ingredient. After 28 days postdosing, rats were anesthetized to collect blood samples then euthanized to collect liver and kidney tissue specimens. The results showed marked changes in walking, body tension, alertness, and head movement with a significant reduction in rats’ body weight in both IM and HFM receiving groups. Significant increases in MDA levels and decrease of GHS levels were recorded in liver and kidney homogenates of either IM or HFM groups. Liver and kidney tissues obtained from both pesticide receiving groups showed extensive histopathological alterations with a significant increase in the serum levels of ALT, AST, urea, and creatinine and a decrease in total proteins, albumin, and globulin levels. In addition, there was upregulation of the transcript levels of casp-3, JNK, and HO-1 genes with strong immunopositivity of casp-3, TNF-ὰ, and NF-KB protein expressions in the liver and kidneys of rats receiving either IM or HFM compared with the control group. In all studied parameters, HFM caused hepatorenal toxicity more than those induced by IM. We can conclude that each IM and HFM provoked liver and kidneys damage through overproduction of ROS, activation of NF-KB signaling pathways and mitochondrial/JNK-dependent apoptosis pathway.

scholarly journals Protective effect of aqueous extract of Lophira lanceolata leaf against cisplatin-induced hepatorenal injuries and dyslipidemia in Wistar rats

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Raphael John Ogbe ◽  
Samuel Peter Agbese ◽  
Adakole Hyacinth Abu
Keyword(s):  
Protective Effect ◽  
Aqueous Extract ◽  
Wistar Rats ◽  
Ldl Cholesterol ◽  
Control Group ◽  
Group I ◽  
Medicinal Plant Extracts ◽  
Liver And Kidney ◽  
Lophira Lanceolata ◽  
Group Ii

Abstract Background Hepatorenal injuries and dyslipidemia are common global health challenges but medicinal plant extracts may have potential to prevent them. Thus, this study evaluated the protective effect of aqueous extract of Lophira lanceolata leaf (LLE) against cisplatin-induced hepatorenal injuries and dyslipidemia in albino Wistar rats. Methods Thirty rats were randomly divided into 6 groups of 5 rats each. Group I rats received distilled water and served as control, group II rats were given 5 mg/kg cisplatin (CIS) intraperitoneally, groups III and IV rats were treated with 200 and 400 mg/kg LLE respectively for 26 days by oral gavages while groups V and VI rats were treated with 200 and 400 mg/kg LLE respectively, followed by CIS on the 21st day as in group II. About 24 h after treatment, blood was collected from the rats; then serum was separated and used for estimations of biochemical parameters. The kidney and liver of rats were removed, rinsed in normal saline, stored in 10% formalin and used for histological analyses. Results The biomarkers of hepatic (Aminotransferases, Alkaline phosphatase and Bilirubin) and renal (urea and creatinine) injuries, and dyslipidemia (Total cholesterol, triglycerides and LDL-cholesterol) significantly (p < 0.05) increased in the rats exclusively exposed to cisplatin when compared with normal control. However, treatment of cisplatin-exposed rats with 200 and 400 mg/kg LLE significantly (p < 0.05) reduced the levels of these biomarkers of hepatorenal injuries and dyslipidemia when compared with cisplatin control. Photomicrographs showed pathological signs in the liver and kidney of rats exclusively exposed to cisplatin, but there was moderate protection of these tissues in the rats treated with LLE and cisplatin. Conclusion The current findings have shown that Lophira lanceolata leaf extract may provide moderate protection against cisplatin-induced hepatorenal injuries and dyslipidemia in albino Wistar rats.

scholarly journals A study on comparison of memory boosting and regaining effects of oral administration of peppermint and coriander in wistar albino rats.

2014 ◽  
Vol 3 (1) ◽  
pp. 13-19
Author(s):  
Sai Sailesh Kumar ◽  
Jasira V k Mohamed ◽  
JK Mukkadan
Keyword(s):  
Oral Administration ◽  
Task Performance ◽  
Wistar Rats ◽  
Memory Performance ◽  
Oral Intake ◽  
Control Group ◽  
Albino Rats ◽  
Physiological Measures ◽  
Behavioral Task ◽  
Wistar Albino Rats

The present study provides further evidence that oral administration of peppermint and coriander extracts are having memory boosting and memory regaining effects. The present study was undertaken to compare memory boosting and regaining effects of oral administration of peppermint and coriander extracts in adult wistar rats. Here we investigate the influence of oral intake of peppermint and coriander extracts on behavioral task performance by using T-maze and radial arm maze and physiological measures relative to a milk control group. When coriandrum and peppermint groups are compared, the memory boosting and regaining effects of peppermint is significant in R-maze, whereas memory boosting and regaining effects of coriandrum is significant in T-maze. We conclude that oral administration of peppermint and coriander extracts are having memory boosting and memory regaining effects. Hence we recommend peppermint and coriander can be used as a remedy in the management of Alzheimer's disease and we also recommend further research in this area by investigating compound metabolism to optimize quantification of memory performance following peppermint and coriander ingestion. CBMJ 2014 January: Vol. 03 No. 01 P: 13-19

scholarly journals Histomorphologic Effect of Citrus aurantiifolia against Cadmium Chloride Induced Toxicity in the Liver and Kidney of Adult Wistar Rats

2020 ◽  
pp. 1-7
Author(s):  
E. U. Eric ◽  
F. O. Adiela
Keyword(s):  
Liver Injury ◽  
Protective Effect ◽  
Cadmium Chloride ◽  
Wistar Rats ◽  
Health Science ◽  
Albino Rats ◽  
Central Vein ◽  
Lime Juice ◽  
Liver And Kidney ◽  
Citrus Aurantiifolia

Aim: The study aimed to evaluate the protective effect of Citrus aurantiifolia against cadmium chloride toxicity induced in the liver and kidney of Albino Wistar rats histologically. Methods: Twenty five adult female Albino rats weighing 150±11 to 200±50 g were used for the study. The rats were purchased from the Department of Pharmacology, College of Health Science, University Port Harcourt, Rivers State of Nigeria. They were randomly assigned into five groups (A B C D& E) with each group having five rats. Group A (Control) received only food and water for six weeks (6 weeks), Group B received food, cadmium water and treatment with lime juice for six weeks (6 weeks), Group C received food, water and lime juice for three weeks. After three weeks, they stopped lime and water and were induced with cadmium chloride for 3 weeks, Group D received food, cadmium chloride for 3 weeks and after 3 week they were given normal water and treated with lime for another 3 weeks, Group E received food and cadmium water for six weeks. At the end of the treatment, the liver and the kidney of each sacrificed rat were processed for paraffin sectioning and stained with Harris hematoxylin and eosin. Results: Photomicrographs of Groups B and C which were induced with Cadmium Chloride and treated with citrus shows area of central necrosis and central vein congested with red blood cells and also the presence of inflammatory cells which are features of liver injury can be seen. Conclusion: There was no significant protective effect of Citrus aurantiifolia against cadmium-induced liver injury in Albino Wistar rats. Also, there was no significant effect of cadmium-induced toxicity on the kidney of the Albino Wistar rat.

scholarly journals Evaluation of Antioxidant and Wound Healing Effects of Alcoholic and Aqueous Extract ofOcimum sanctum Linnin Rats

2008 ◽  
Vol 5 (1) ◽  
pp. 95-101 ◽  
Author(s):  
Somashekar Shetty ◽  
Saraswati Udupa ◽  
Laxminarayana Udupa
Keyword(s):  
Wound Healing ◽  
Aqueous Extract ◽  
Antioxidant Properties ◽  
Ocimum Sanctum ◽  
Control Group ◽  
Albino Rats ◽  
Impaired Wound Healing ◽  
Wistar Albino Rats ◽  
Different Doses ◽  
Group 1

In recent years, oxidative stress and free radicals have been implicated in impaired wound healing.Ocimum sanctum(O. sanctum), a plant widely used in Ayurveda, possesses anti-inflammatory and antioxidant properties. The present study was undertaken to assess the potential of alcoholic and aqueous extracts in wound healing in Wistar albino rats. The rats were divided into five groups of six animals each. Group 1 is normal wounded control and the other four groups were treated with two different doses each of alcoholic and aqueous extract ofO. sanctum. The wound healing parameters were evaluated by using incision, excision and dead space wounds in extract-treated rats and controls. Both the doses of alcoholic and aqueous extract significantly increased wound breaking strength, hydroxyproline, hexuronic acid, hexosamines, superoxide dismutase, catalase, reduced glutathione and significantly decreased percentage of wound contraction and lipid peroxidation when compared with the control group. The results suggest thatO. sanctumhas antioxidant properties, which may be responsible and favorable for faster wound healing and this plant extract may be useful in the management of abnormal healing and hypertropic scars.

scholarly journals AN EXPERIMENTAL STUDY TO EVALUATE THE HEPATOPROTECTIVE ACTIVITY OF NILITANDULIYADI LEHA IN PARACETAMOL INDUCED HEPATOTOXICITY IN WISTAR ALBINO RATS

2021 ◽  
Vol 10 (5) ◽  
pp. 127-130
Author(s):  
Sreekala Vijayan ◽  
Jugal Kishore
Keyword(s):  
Biochemical Parameters ◽  
Wistar Rats ◽  
Hepatoprotective Activity ◽  
Albino Rats ◽  
Test Drug ◽  
Protective Activity ◽  
Standard Drug ◽  
Liver And Kidney ◽  
Paracetamol Toxicity ◽  
Wistar Albino Rats

Background: Paracetamol toxicity is currently the single most important cause for acute liver failure and is associated with significant number of deaths. Nilitanduliyadi leha is one among the formulation explained in the context of Vishahara yogas (anti-poisonous formulations) in the text Vishavaidya Jyotsnika. Objectives: To experimentally evaluate the hepatoprotective activity of nilitanduliyadi leha in paracetamol induced hepatotoxicity in Wistar albino rats. Methods: Albino Wistar rats of either sex weighing 200 – 250, g were selected and divided into four groups of six animals in each group (n = 6). Treatment was given for 7 days. Blood was drawn and sent for tests and important organs like liver and kidney were dissected out, cleaned to remove extraneous tissues, blotted to remove blood stain and weighed. A piece of liver tissue was preserved in 10% formalin for histopathological processing. Results: The formulation has helped in balancing the biochemical parameters studied almost as efficiently as the standard drug. In the antioxidant study also, the drug has given good results and shows even slightly more effective than the standard drug. The histopathology study also reveals mild protection and regeneration of tissues by the effect of test drug. Conclusion: This present study proves that the formulation is having a comparable hepato protective activity with that of silymarin.

Effect of Propolis-Supplemented Diet on Body Composition and Endocrine Function of Adipose Tissue

2020 ◽  
Vol 19 (2) ◽  
pp. 217-221
Author(s):  
Maria Jesús Lisbona-González ◽  
Candela Reyes-Botella ◽  
Esther Muñoz-Soto ◽  
Maria Victoria Olmedo-Gaya, ◽  
Jorge Moreno-Fernandez ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Body Composition ◽  
Adipose Tissue ◽  
Endocrine Function ◽  
Control Group ◽  
Albino Rats ◽  
Standard Diet ◽  
Reduced Body ◽  
Esterified Fatty Acids ◽  
Wistar Albino Rats

Adipose tissue is an endocrine organ and has central role in interaction with other organs or tissues while propolis can induce lipolysis. Therefore, the aim of this study is to provide detailed information about adipose tissue homeostasis modifications and body composition during propolis supplement consumption. Twenty male Wistar albino rats (8 weeks) were divided into two groups of 10 animals each and fed for 90 days with two different types of diets: standard for the control group (diet C) and standard diet + 2% propolis (diet P). Thyroid hormones did not show differences, while ghrelin and adiponectin decreased in the group that was fed propolis. Insulin, leptin, and non-esterified fatty acids also increased along with reduced body weight and fat, in addition to increased lean mass when propolis was in the diet. We conclude that propolis could decrease ghrelin and adiponectin but increase non-esterified fatty acids and insulin secretion, which improves body composition.

Sign in / Sign up

Export Citation Format

Share Document