scholarly journals AKTIVITAS ANTIOKSIDAN BERBAGAI JENIS MAKROALGA DI PANTAI SEPANJANG, KABUPATEN GUNUNGKIDUL, YOGYAKARTA (Antioxidant Activities in Various Types of Macroalgae at Sepanjang Beach, Gunungkidul District, Yogyakarta)

2021 ◽  
Vol 17 (2) ◽  
pp. 130-137
Author(s):  
Heny Budi Setyorini ◽  
Amallia Puspitasari
Keyword(s):  
Antioxidant Activities ◽  
Ulva Lactuca ◽  
Gracilaria Verrucosa ◽  
Palmaria Palmata ◽  
Sample Collection ◽  
Gelidiella Acerosa ◽  
Purposive Sampling ◽  
Western Area ◽  
Ic50 Value ◽  
Dpph Method

Penelitian ini bertujuan untuk mengetahui aktivitas antioksidan berbagai jenis makroalga di Pantai Sepanjang, Kabupaten Gunungkidul, Yogyakarta. Penelitian ini dilaksanakan di Pantai Sepanjang, Kabupaten Gunungkidul, Yogyakarta pada bulan Agustus 2020. Jenis makroalga yang ditemukan pada saat penelitian antara lain Ulva lactuca, Palmaria palmata, Sargassum crassifolium, Gelidium spinosum, Gelidiella acerosa, dan Gracilaria verrucosa. Metode yang digunakan dalam penelitian ini adalah deskriptif eksploratif. Pengambilan sampel makroalga dilakukan secara purposive sampling meliputi bagian timur, tengah dan barat Pantai Sepanjang. Analisis aktivitas antioksidan menggunakan metode DPPH (1,1-diphenyl-2-picrylhydrazyl). Hasil analisis menunjukan bahwa potensi aktivitas antioksidan tertinggi terdapat pada Gelidiella acerosa yang berada di bagian tengah Pantai Sepanjang dengan nilai IC50 sebesar 22,407 g/ml. This research aimed to analyze antioxidant activities from various types of macroalgae. Sample collection was taken at Sepanjang Beach, Gunungkidul District, Yogyakarta in August 2020. Various types of macroalgae were found are: Ulva lactuca, Palmaria palmata, Sargassum crassifolium, Gelidium spinosum, Gelidiella acerosa, and Gracilaria verrucosa. A method of descriptive exploratory was used in this research. Macroalgae samples were collected by purposive sampling from the eastern, central and western area of Sepanjang Beach. Antioxidant activities were carried out using DPPH method. The results showed that fresh Gelidiella acerosa which is located in the middle of Sepanjang Beach, has the highest antioxidant activities with IC50 value 22.407 g ml-1.

scholarly journals KANDUNGAN PROTEIN DAN KARBOHIDRAT PADA MAKROALGA DI PANTAI SEPANJANG, YOGYAKARTA

2021 ◽  
Vol 13 (2) ◽  
pp. 283-293
Author(s):  
Heny Budi Setyorini ◽  
Amallia Puspitasari
Keyword(s):  
Gracilaria Verrucosa ◽  
Palmaria Palmata ◽  
Gelidiella Acerosa ◽  
Purposive Sampling

Kandungan protein dan karbohidrat pada makroalga diperlukan untuk mengoptimalkan pemanfaatan makroalga di Pantai Sepanjang. Penelitian ini bertujuan untuk mengetahui kandungan protein dan karbohidrat pada makroalga di Pantai Sepanjang, Yogyakarta. Penelitian ini dilakukan pada bulan Agustus-September 2020 di Pantai Sepanjang, Yogyakarta. Materi penelitian ini meliputi makroalga jenis Ulva lactuta, Palmaria palmata, Sargassum crassifolium, Gelidium spinosum, Gelidiella acerosa, dan Gracilaria verrucosa. Sampel makroalga secara purposive sampling pada zona intertidal Pantai Sepanjang. Analisis kandungan protein menggunakan metode Biuret, sedangkan analisis kandungan karbohidrat menggunakan metode by difference. Hasil menujukkan bahwa kandungan protein dan karbohidrat tertinggi terdapat pada G. spinosum di bagian tengah Pantai Sepanjang masing-masing sebesar 3,08% dan 19,38%. Berdasarkan hasil tersebut, G. spinosum memiliki potensi untuk dikembangkan dalam berbagai produk olahan dengan penelitian lebih lanjut.

scholarly journals Evaluation of Antioxidant Activities From Ethyl Acetate Fraction of Curry Leaf Using DPPH Method

Elkawnie ◽  
2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Ulil Amna ◽  
Halimatussakdiah Halimatussakdiah ◽  
Furqan Nur Ihsan ◽  
Puji Wahyuningsih
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Ethyl Acetate Fraction ◽  
Murraya Koenigii ◽  
Chromatography Method ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ethyl Acetate Extracts

Abstract: Curry Plant (Murraya koenigii (Linn.) Spreng) is one of the plants that thrive in Indonesia. Curry contains secondary metabolites of alkaloids and flavonoids that have the potential as antioxidants. Antioxidants can reduce the activity of free radicals that can cause degenerative diseases such as cancer, coronary heart disease, and premature ageing of body cells by donating the protons to free radical compounds. This study aimed to determine the antioxidant activity and identify the active compound class of ethyl acetate fraction from curry leaves. Separation of secondary metabolites of ethyl acetate extracts was carried out by column chromatography method, which obtained 5 different fractions. The antioxidant activity of fractions A, B, C, D, and E was tested using the DPPH (2,2-diphenyl-1-picrylhydrazyl) method with variations concentrations of 25, 50, 100, 200, and 400 ppm, and ascorbic acid was used as a comparison which measured at a wavelength of 517 nm. The results showed that fractions A and E have weak antioxidant activities, while fractions B, C, and D showed moderate antioxidant activities. The highest antioxidant activity was found in fraction B with an IC50 value of 341.38 ppm, where the fraction showed the presence of secondary metabolites of alkaloids and terpenoids.Abstrak: Tanaman kari ((Murraya koenigii (Linn.) Spreng) merupakan salah satu tanaman yang tersebar di Indonesia. Kari mengandung senyawa metabolit sekunder alkaloid dan flavonoid yang memiliki potensi sebagai antioksidan. Antioksidan dapat mengurangi aktivitas radikal bebas yang dapat menyebabkan penyakit degeneratif seperti kanker, penyakit jantung coroner dan penuaan sel dengan cara menyumbangkan proton pada senyawa radikal bebas. Tujuan dari penelitian ini adalah untuk menentukan aktivitas antioksidan dan mengidentifikasi kelas senyawa aktif fraksi etil asetat dari daun kari. Pemisahan metabolit sekunder dari ekstrak etil asetat dilakukan menggunakan metode kromatografi kolom dengan menghasilkan 5 fraksi yang berbeda. Aktivitas antioksidan dari fraksi A, B, C ,D ,dan E dilakukan menggunakan metode DPPH (1,1-diphenil-2-pikrilhydrazil) dengan variasi konsentrasi 25, 50, 100, 200, dan 400 ppm dan asam askorbat digunakan sebagai perbandingan yang diukur pada panjang gelombang 517 nm. Hasil menunjukkan bahwa fraksi A dan E memiliki aktivitas antioksidan yang lemah, sedangkan fraksi B, C, dan D menunjukkan aktivitas antioksidan sedang. Aktivitas antioksidan yang paling tinggi ditemukan pada fraksi B dengan IC50 341,38 ppm. Fraksi tersebut menunjukkan adanya kandungan senyawa metabolit sekunder alkaloid dan terpenoid. 

scholarly journals PERTUMBUHAN DAN AKTIVITAS ANTIOKSIDAN DARI MIKROALGA

Jurnal BIOMA ◽  
2015 ◽  
Vol 11 (1) ◽  
pp. 12
Author(s):  
MONIKA RAFAELINA ◽  
YOSWITA RUSTAM ◽  
SRI AMINI
Keyword(s):  
Antioxidant Activity ◽  
Cell Density ◽  
Antioxidant Activities ◽  
Outdoor Culture ◽  
Chlorella Sp ◽  
Randomized Design ◽  
Ic50 Value ◽  
Dpph Method ◽  
Univariate Test ◽  
Indoor And Outdoor

ABSTRACT Microalgaebiomass containschemicalcomponentssuch as vitamin, proteins, ω-3 and ω-6 polyunsaturated fatty acid and pigments. components with antioxidant activities can be found in only a few species of algae. The potential species of microalgae who have compounds act as antioxidant are Porphyridium cruentum and Chlorella sp. This study aimed to know the growth and antioxidant activity of P. cruentum and Chlorella sp. with the treatment of different cultures. Experimental method and completely randomized design was used as the research method. Antioxidant activity (Ic50 value) was measured by DPPH method (2,2-diphenyl-1-pikrilhidrazil). SPSS 16.0 is used to analyze the data with General Linier Model (GLM) Univariate. The results of GLM Univariate test for cell density showed that there was significant different for cell density in species factors (p= 0,000), but there was no significant different for cell density in culture factors (p= 0,375), and GLM Univariate test for antioxidant activity showed that no significant different for antioxidant activity in species factors (p= 0,522) but siginificant different for antioxidant activity in culture factors (p= 0,001). There was significant different for growth in P. cruentum and Chlorella sp. but no significant different in indoor and outdoor culture, as well as there was no significant different antioxidant activity in two species but significant different in indoor and outdoor culture.   Keywords: growth, antioxidant activity, indoor cultivation, outdoor cultivation.

scholarly journals BIOACTIVE COMPOUNDS ANALYSIS AND ANTIOXIDANT ACTIVITIES OF Tinospora crispa MIERS STEM EXTRACT

2020 ◽  
Vol 1 (40) ◽  
pp. 58-69
Author(s):  
Tuan Pham Nguyen ◽  
Lam Hong Bang ◽  
Tran Thi Bao Nguyen ◽  
Tu Pham Nguyen
Keyword(s):  
Bioactive Compounds ◽  
Antioxidant Activities ◽  
Raw Materials ◽  
Tannin Content ◽  
Immersion Method ◽  
Potential Source ◽  
Ic50 Value ◽  
Dpph Method ◽  
Biological Compounds ◽  
Stem Extract

The study was conducted to analyze some of the bioactive compounds and the antioxidant capacity of Tinospora crispa stem extract. Tinospora crispa stem extract is a prerequisite for the production of products capable of supporting and treating diseases. Tinospora crispa stem extract is made by immersion method withsolvents (water, ethanol 80o and methanol)  and combined with ultrasound. Antioxidant activities were tested using DPPHmethods and phenolic, flavonoid, polysaccharide, tannin content were determined by the spectrophotometer method. The results showed that the moisture content was 61.09% and high extraction efficiency of Tinospora crispa stem were between 3.69% and 6.95% respectively. The extract of Tinospora crispa stem contains the presence of biological compounds such as alkaloids, flavonoids, saponin, steroids, tannins and phenols. The phenolic, flavonoid, polysaccharide and tannin content of Tinospora crispa stem were318.91 mg gallic acid/g dry; 36.71 mg quercetin/g dry; 10.38 mg GE/g dry and 38.42 mg tannic acid/g dry respectively. Tinospora crispa stem has antioxidant ability by DPPH method with IC50 value of water, ethanol 80o and methanol 113.69 mg/mg; 89.12 mg/mg; 62.19 mg/mg, respectively. Thus, the Tinospora crispastem extract had bioactive compounds and antioxidant activities, is a potential source of raw materials for research and applications. 

scholarly journals Ultrasonic Extraction of Nothaphoebe Coriacea Bark and Its Antioxidant Evaluation

2021 ◽  
Vol 4 (2) ◽  
pp. 52-57
Author(s):  
Salsabila Aqila Putri ◽  
Ratih Febrianti ◽  
Khoirun Nisa ◽  
Sunardi Sunardi
Keyword(s):  
Antioxidant Activities ◽  
Ethanol Extract ◽  
Bioactive Compound ◽  
Extraction Methods ◽  
Chloroform Fraction ◽  
Ultrasound Assisted Extraction ◽  
Hexane Extraction ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ultrasound Assisted

Nothaphoebe coriacea (in Kalimantan, Indonesia, commonly known as gemor) is a native wetland-plants that can grow well in swampy areas. This tree is distributed throughout South and Central Kalimantan, Indonesia. The bark of Nothaphoebe coriacea is used for bioactive compound source for larvacide, mosquito repellent, hio and glue. The objective of this research was to investigate the ultrasound-assisted extraction methods for the extraction of antioxidants compounds of Nothaphoebe coriacea. The barks were subjected to ultrasound‐assisted ethanolic and hexane extraction followed by fractionation (using ethyl acetate, chloroform, and hexane) and the antioxidant activity test was performed. The activity of antioxidant was carried out through a DPPH method. The highest antioxidant activities are shown in ethanol extract with IC50 value of 72.36 μg/mL and chloroform fraction of ethanol extract with IC50 value of 54.18 μg/mL.

Physiochemical properties and antioxidant potential of Persea Americana seed oil

2019 ◽  
Author(s):  
Chem Int
Keyword(s):  
Seed Oil ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Acid Value ◽  
Persea Americana ◽  
Antioxidant Potential ◽  
Physiochemical Properties ◽  
Physiochemical Parameters ◽  
Ic50 Value

Oil extracted from Persea Americana seed was assayed for its physiochemical properties and antioxidant potential using various standard methods. The oil content of the seed was found to be < 10%. Brownish-red color oil was liquid at room temperature, with specific gravity of 0.91±0.02 g/mL. Other physiochemical parameters determined were; acid value (4.51±0.08 mgKOH/g), %FFA (2.26±0.08), peroxide value (2.40±0.57 mgO2/Kg), ester value (31.26±0.03 mgKOH/g), saponification value (35.76±0.07 mgKOH/g) and iodine value (23.5±0.07). The results of the antioxidant activities of the seed oil showed that the flavonoid content (80.00±1.41 mgQE/g) was ~10 folds higher than the phenolic content (8.27±0.06 mgGAE/g). The DPPH radical scavenging value was found to be 51.54±0.25% with an IC50 value of 4.68±0.02 mg/mL and reducing power with an average absorbance of 0.85±0.01 and an IC50 value of 0.001±0.02 mg/mL. Gallic acid showed better antioxidant activities than the oil studied. The results obtained in this study showed that Persea Americana seed oil has nutritional, industrial as well as medicinal potentials.

Comparison of Hypoglycemic and Antioxidant Activities of the Compounds Isolated from Ficus hispida Linn. and Ficus pomifera Wall

2020 ◽  
Vol 10 (5) ◽  
pp. 621-630
Author(s):  
Ashalata Nepram ◽  
Sujata Wangkheirakpam ◽  
Warjeet S. Laitonjam
Keyword(s):  
Methanol Extract ◽  
Antioxidant Activities ◽  
Tolerance Test ◽  
Chloroform Extract ◽  
Diabetic Rats ◽  
Hypoglycemic Activity ◽  
North East India ◽  
North East ◽  
Dpph Method ◽  
Ficus Hispida

Background: Traditional knowledge has been a legacy of the past to the present. Barks of Ficus hispida Linn. and leaves of Ficus pomifera Wall. (Moraceae) have been used traditionally for the treatment of diabetes in North-east India and many other places. As many drugs have been developed from traditional plants, the authors have taken up the plants for the study of hypoglycemic activity. Objective: To investigate the hypoglycemic activities of the triterpenoids isolated from the plants and their antioxidant activities. Methods: The bioactive compounds were determined by biochemical analysis, antioxidant activity using DPPH method. Hypoglycemic activity was detected using glucose tolerance test in normal rats and alloxan induced diabetic rats with Gliclazide as standard. Results: The biochemicals and trace elements were present in appreciable amounts. Triterpenoids, (1-5), from F. pomifera and 19-hydroxyphlogacantholide (6), 3-O-[ß-D-glucopyranosyl-(1’→2’)-α- L-rhamnopyranosyl-phlogacanthoside] (7) and galanolactone (8) along with stigmasterol (9), stigmasta- 5,22-dien-7-on-3ß-ol (10), 5-(decahydro-1,1,4a-trimethyl-6-methylene-5-yl)-3-methylpent-2- enal (11), stigmasterol glucoside (12) and stigmast-4-en-3-one (13) from F. hispida Linn., respectively, were isolated. The different extracts of the barks and leaves of these plants along with the isolated compounds had antioxidant and hyploglycemic activities. Conclusions: The five triterpenoids (1-5) were isolated from the methanol extract of the leaves of F. pomifera, and compounds (6-13) were isolated from the chloroform extract of the barks of F. hispida. Methanol extract of the leaves of F. pomifera and the chloroform extract of the barks of F. hispida; compounds (1-13) isolated from these two plants reduced DPPH free radicals in a concentrationdependent manner. It was also observed that the methanol and chloroform extracts of the plants, F. pomifera and F. hispida respectively, and the compounds (1, 6 & 7) exhibited anti-diabetic properties and also caused a highly significant reduction in the blood glucose levels of normal rats.

PREDIKSI PERINGKAT OBLIGASI SYARIAH DI INDONESIA

2014 ◽  
Vol 11 (3) ◽  
pp. 1-15
Author(s):  
Ike Arisanti ◽  
Isti Fadah ◽  
Novi Puspitasari
Keyword(s):  
Profit Sharing ◽  
Sample Collection ◽  
Purposive Sampling ◽  
Financial Factors ◽  
Financial Factor ◽  
Independent Variable ◽  
Rating Prediction ◽  
Collection Methods

This study purposes to analyze the influence of financial and non financial factors to prediction of the rating islamic bond in indonesia. The study used independent variable,that is financial factor (growth, size, profit sharing/fee, liquidity) and non financial factor ( secure and maturity) and dependent variable that is the rating of islamic bond. This study applied logistic regresion analysis with sample collection methods using purposive sampling. After selecting fixed criterias, there were 25 islamic bonds chosen with the numbers of 75 investigation from periods of 2010-2012. The result of this study showed that significantly effect the variable growth (X1) , size(X2), profit sharing/ fee (X3), liquidity (X4), secure (X5), maturity (X6) simultaneously to the rating prediction of islamic bond in indonesia. Partially, variable variables of growth (X1) , size (X2), profit sharing/ fee (X3) which referred not significant affecting to the rating prediction of islamic bond in indonesia. Meanwhile, variables of liquidity (X4), secure (X5), maturity ( X6) referred significant affecting to the rating prediction of islamic bond in indonesia.

scholarly journals FRACTIONATION OF THE N-HEXANE EXTRACT OF GARCINIA BANCANA MIQ. (MANGGIS HUTAN) LEAVES AND ITS ANTIOXIDANT ACTIVITY BASED ON 1,1-DIPHENYL-2- PICRYLHYDRAZYL AND FERRIC REDUCING ANTIOXIDANT POWER ASSAYS

2018 ◽  
Vol 10 (1) ◽  
pp. 407
Author(s):  
Dewi Kumala Putri ◽  
Berna Elya ◽  
Nuraini Puspitasari
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Hexane Extract ◽  
Phytochemical Screening ◽  
Active Fraction ◽  
Leaf Extracts ◽  
Antioxidant Power ◽  
Dpph Method ◽  
Ferric Reducing Antioxidant Power

Objective: To assess the antioxidant activity from another part of the plant, in this study, leaf extracts in n-hexane were fractionated.Methods: Ten fractions were obtained and tested in vitro for antioxidant activity using two methods, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferricreducing antioxidant power (FRAP), to identify the most active fraction.Results: The IC50 of the most active fraction was 36.24 μg/mL using the DPPH method, and the EC50 was 39.54 μg/mL using the FRAP method. Themost active fraction was also shown to contain terpenoids.Conclusion: The most active fraction of an n-hexane extract of the leaves of Gacinia bancana Miq., which was tested by both DPPH and FRAP methodshad antioxidant activities with IC50 and EC50 values of 36.2482 μg/mL and 39.5442 μg/mL, respectively. Phytochemical screening showed that activefraction contains terpenoids.

scholarly journals Ultrasonically-Extracted Marine Polysaccharides as Potential Green Antioxidant Alternatives

Proceedings ◽  
2020 ◽  
Vol 67 (1) ◽  
pp. 23
Author(s):  
Hanaa L. Essa ◽  
Hania A. Guirguis ◽  
Mayyada M. H. El-Sayed ◽  
Dalia Rifaat ◽  
Mohamed S. Abdelfattah
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Algal Species ◽  
Avicennia Marina ◽  
Ulva Lactuca ◽  
Biologically Active ◽  
Sulfated Polysaccharides ◽  
Total Phenolic ◽  
Sugar Substitutes ◽  
Pharmaceutical Industries

Marine-extracted sulfated polysaccharides (SPs) have been the subject of myriad research since they are considered an eco-friendly source of biologically active compounds. Meanwhile, food and pharmaceutical industries are urgently producing natural sugar substitutes and antioxidants as alternatives to synthetic ones which are associated with cytotoxicity and safety issues. This study assesses the potential of using marine SPs obtained via the ultrasonic-assisted extraction of different marine species, to utilize them as antioxidant sugar substitutes. The carbohydrate, total phenolic contents and antioxidant activities were measured for SP extracts of the algal species of Ulva lactuca, Jania rubens and the marine plant mangrove Avicennia marina. These SPs were structurally elucidated by Fourier Transform Infrared (FTIR) spectroscopic and high-performance liquid chromatography (HPLC) analyses. The results revealed that SPs’ highest yield percent was obtained from Ulva lactuca, 5.50 ± 0.25%. The SPs of Avicennia marina had the highest carbohydrate content, 44 ± 1% and antioxidant activity, 78.85 ± 0.06 at the 100 μg/mL concentration and 89.50 ± 0.21 at the 250 μg/mL concentration. Meanwhile, the highest phenolic content was exhibited by algal SPs obtained from Jania rubens, 132.60 ± 2.50 mgGa/g. Results also showed that all extracts have potent antioxidant activity, while the highest antioxidant activity belonged to the SPs of Avicennia marina owing possibly to their balanced glucose and galactose contents as measured by HPLC. This work emphasizes the need to consider sulfated polysaccharides from marine sources for their antioxidant activity and to correlate it with their monosaccharide content to determine the effect of reducing sugar concentration on the antioxidant activity.

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