scholarly journals The Effect of Glutathione on Serum Malondialdehyde (MDA) Level in Retinopathy of Prematurity Rat Models

2020 ◽  
Vol 6 (3) ◽  
pp. 86-88
Author(s):  
Puspita Kusuma Dewi ◽  
Maharani Cahyono ◽  
Riski Prihatningtias ◽  
Liana Ekowati ◽  
Arief Wildan
Keyword(s):  
Oxidative Stress ◽  
Laboratory Condition ◽  
Retinopathy Of Prematurity ◽  
Oxidative Stress Marker ◽  
Control Group ◽  
Study Group ◽  
Rat Models ◽  
Post Test ◽  
Anti Oxidant ◽  
And Control

Introduction Retinopathy of prematurity (ROP) is the leading cause of blindness in newborn babies worldwide. The benefit of anti-oxidant was investigated for ROP cases by assessing its effect on the oxidative stress of the tissues. Glutathione is a primary endogenous in human body and its supplementation has been discovered for its benefits towards some ocular diseases. This study aims to understand the effect of glutathione on oxidative stress marker, serum Malondialdehyde (MDA), in ROP rat models.Materials and methods This was an experimental study with post test only controlled group design. Sixteen Wistar rats that met our study criterias were divided into two groups, study group and control group. The study group were exposed to 95% oxygen for 4 hours / day followed by normoxic laboratory condition for 20 hours. Glutathione 1.5 mg / day were injected intramuscularly to rats in study group. The control group was exposed to 95% oxygen followed by normoxic laboratory condition with the same manner, and did not received glutathione. This cycle was repeated for 14 days. Both groups were settled in a room temperature settings on days 15-22. Serum sampel was collected from retroorbital vein. The malondialdehyde level was analyzed using MDA analyser kit.Results MDA level was found significantly higher in study group compared to control group (546.99 ng/ml vs 201.51 ng/ml, respectively, p 0,001).Conclusion Our study demonstrated a higher MDA levels in ROP rat models given glutathione injection compared to the control group.

scholarly journals The effect of 8-week thera-band exercises on male swimmers’ 100 m freestyle swimming performance

2017 ◽  
Vol 14 (4) ◽  
pp. 3950 ◽  
Author(s):  
Ahmet Gönener ◽  
Utku Gönener ◽  
Ozan Yılmaz ◽  
Tuğçe Horoz ◽  
Deniz Demirci
Keyword(s):  
Swimming Performance ◽  
Training Programme ◽  
Rank Test ◽  
Control Group ◽  
Study Group ◽  
Test Results ◽  
Signed Rank ◽  
Post Test ◽  
Signed Rank Test ◽  
And Control

The aim of this study is to investigate 8 weeks Thera-Band trainings' effects on male swimmers' 100 m freestyle swimming performance.The study group is created by 20 (n = 20) licenced male athletes that had trained at least 3 days in a week and have been active in swimming sport at least 3 years in Gebze Genclerbirligi Swimming Club 20 (n = 20). Athletes were divided into experiment group (n = 10) and control group (n = 10) randomly. Training programme was applied to the study group for 55-60 minutes for 3 days on alternate days and times when the club does not have swimming training. 12 different Thera-Band trainings were applied for 40-45 mins and each set was 15 minutes.Mann-Whitney U test was used to analyze differences between groups and Wilcoxon signed rank test was applied for analyzing the differences of intra-groups. SPSS 21.0 Statistics package software was used for statistical analyzes. The results show that there are no significantly differences between experimental group's and control group's post test results. (p>0.05). Statistically significant differences are found as a result of intra-group comparison of the experimental group's pre-test and post-test results (p<0.05).Depending on the results obtained after reviewing the literature, it can be concluded that Thera-Band training is effective on the performance improvement of swimmers aged 13-15 years.

scholarly journals DETERMINASI PEMBERIAN MINYAK TRANS TERHADAP STRES OKSIDATIF PADA TIKUS GALUR WISTAR DENGAN MALONDIALDEHIDA PANKREAS SEBAGAI INDIKATOR

Jurnal BIOMA ◽  
2015 ◽  
Vol 11 (2) ◽  
pp. 201
Author(s):  
Maharja Mawali ◽  
Koekoeh Santoso ◽  
Nastiti Kusumorini ◽  
Aryani Sismin Satyaningtijas ◽  
Atin Supiyani
Keyword(s):  
Oxidative Stress ◽  
Fatty Acids ◽  
Trans Fatty Acids ◽  
Wistar Rats ◽  
Unsaturated Fatty Acids ◽  
Oxidative Stress Marker ◽  
Control Group ◽  
Predisposing Factor ◽  
Tbars Assay ◽  
And Control

ABSTRACT Trans fatty acids (TFAs) are the entire unsaturated fatty acids containing one or more double bonds isolated in trans configuration believed as predisposing factor of oxidative stress, excluding conjugated double bond fatty acids. Malondialdehyde (MDA) is a product of lipid peroxidation used as oxidative stress marker. The objective of this study was to see the correlation between TFAs containing-oil (consists of 3-6% TFAs) intake at various administration doses and pancreatic MDA levels in Wistar rats. Twelve rats were grouped according to administration doses (20%, 40%, 60% of given feed total energy and control group). TFAs containing-oil was administered orally once a day for 70 days at given doses by force feeding. Pancreatic MDA were measured using TBARS assay adapted from Singh et al. (2006). Increase of TFAs  containing-oil  administration  dose  caused  insignificant  increase  of  pancreatic MDA level.   Keywords: trans fatty acids, oxidative stress, malondialdehyde, Wistar rats, palm oil

Role of Ischemia Modified Albumin Serum Levels as an Oxidative Stress Marker in Children with Diabetic Ketoacidosis

2019 ◽  
Vol 22 (8) ◽  
pp. 577-581
Author(s):  
Mutlu U. Yazıcı ◽  
Ganime Ayar ◽  
Senay Savas-Erdeve ◽  
Ebru Azapağası ◽  
Salim Neşelioğlu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Intensive Care Unit ◽  
Intensive Care ◽  
Diabetic Ketoacidosis ◽  
Pediatric Patients ◽  
Serum Levels ◽  
Oxidative Stress Marker ◽  
Control Group ◽  
Study Group ◽  
Ischemia Modified Albumin

Aim and Objective: Ischemia modified albumin (IMA) is a biomarker that has been introduced recently for use in the evaluation of oxidative stress. The aim of this study was to measure the ischemia modified albumin serum levels in pediatric patients with diabetic ketoacidosis (DKA) during acidosis and after the patient recovered from acidosis and to compare these with the control group. Materials and Methods: Pediatric patients with Type I diabetes mellitus (T1DM) who were admitted to the pediatric intensive care unit with the diabetic ketoacidosis were assigned as the study group and healthy children who were admitted to the outpatient clinic and decided as healthy after clinic and laboratory evaluation were selected as the control group. IMA and adjusted IMA levels were evaluated in the blood samples from the control group and the study group when admitted first time to the intensive care unit during the acidosis period (DKA before treatment, DKA-BT), and after recovering from acidosis (DKA after treatment, DKA-AT). Results: A total of 24 pediatric patients with diabetic ketoacidosis and 30 healthy control children matching age and sex were included in the current study. The albumin levels in pediatric patients with T1DM during DKA-BT were higher than the albumin levels after acidosis (4.101±0.373, 3.854±0.369 g/dL, respectively) (p<0.05). However, there was no significant difference when these values were compared to the control group. Mean values of IMA and Adj-IMA were statistically higher in DKAAT compared to the control group (0.748±0.150 vs 0.591±0.099, p< 0.001; 0.708±0.125 vs 0.607±0.824, p< 0.001, respectively). IMA and adjusted IMA levels measured after recovered from acidosis were significantly higher compared to the level of IMA during DKA (0.748±0.150 vs 0.606±0.105 as absorbance unit, p<0.001; 0.708±0.125 vs 0.625±0.100, p<0.05, respectively). Conclusion: In children with T1DM, even though acidosis recovered following the treatment in diabetic ketoacidosis, which is an oxidative stress marker, the ischemia modified albumin levels and adjusted ischemia modified albumin levels were high.

Salivary and Serum Antioxidant and Oxidative Stress Markers in Dental Caries

2018 ◽  
Vol 52 (6) ◽  
pp. 565-569 ◽  
Author(s):  
Fatemeh Ahmadi-Motamayel ◽  
Mohammad Taghi Goodarzi ◽  
Ali Mahdavinezhad ◽  
Zohreh Jamshidi ◽  
Mohammad Darvishi
Keyword(s):  
Oxidative Stress ◽  
Dental Caries ◽  
Oral Disease ◽  
Healthy Control Group ◽  
Oxidative Stress Marker ◽  
Control Group ◽  
Control Groups ◽  
Whole Saliva ◽  
Healthy Control ◽  
And Control

Dental caries is the most common, chronic, noncommunicable, preventable oral disease worldwide. Oxidation may play an important role in dental caries initiation and progression. Antioxidants in body fluids protect cells. The aim of this study was to evaluate salivary and serum total antioxidant capacity (TAC) and malondialdehyde (MDA) levels in dental caries. A total of 118 healthy caries-free and caries-active male and female students participated. Caries was detected clinically. Unstimulated whole-saliva samples and blood samples were obtained. Sialochemical analysis was carried out by spectrophotometric assay. Data were analyzed with the Student t test using STATA 11. Salivary and serum TAC levels in the case and control groups did not show any significant differences. Mean salivary MDA levels in the case and control groups were 0.71 ± 0.1 and 0.35 ± 0.06 nmol/mL, respectively. The results showed significantly higher levels of salivary and serum MDA in the case group compared to the healthy control group. The oxidative stress marker was significantly higher in the caries group compared to the healthy control group. Antioxidants were not significantly different between the two groups. MDA can be produced by dental caries, resulting in a decrease in antioxidant levels, causing disease progression. Further studies are necessary to determine whether MDA is the cause or effect of the disease.

Effectiveness of Cardiac Walking on Blood Pressure Among Patients with Acute Coronary Syndrome

2017 ◽  
Vol 9 (2) ◽  
Author(s):  
T. Vidhya ◽  
C. Kanniammal ◽  
Jaideep Mahendra ◽  
G. Valli
Keyword(s):  
Blood Pressure ◽  
Acute Coronary Syndrome ◽  
World Health ◽  
Control Group ◽  
Study Group ◽  
Coronary Syndrome ◽  
Significant Difference ◽  
The World ◽  
Post Test ◽  
And Control

Background: Cardiovascular diseases (CVD) are the leading cause of the mortality in urban and rural India and foremost causes of the death worldwide. Hypertension is a major risk condition that plays a pivotal role regarding the complications for those diagnosed with Acute Coronary Syndrome. According to the World Health Organization (2012), hypertension is the number one cause of mortality in the world and it is a major risk factor for cardiac diseases and stroke. Objectives: i) To identify the level of blood pressure (BP) in pre and post test among study and control group of patients with acute coronary syndrome. ii) Determine the effect of cardiac walking on blood pressure among patients with ACS. iii) Associate the selected back ground variables with the level of systolic BP in the post test among study and control group. Methods: A quasi experimental design was adopted for this study. There are 50 Patients diagnosed with ASC admitted for the treatment and attending cardiac OPD were selected by purposive sampling technique of non- probability type. The intervention cardiac walking given for a period of one month to the study group. Findings: The comparison of before and after systolic and diastolic blood pressure (BP) within study group depicted statistically significant difference at **p less than 0.001 but not in control group. The comparison of post systolic and diastolic BP between study and control group inferred that there was statistically significant difference at **p less than 0.001. Conclusion: Cardiac walking is most effective way to stabilize the blood pressure among patients with ACS in order to prevent further complications such as stroke.

scholarly journals Oxidative Stress and Antioxidant Status in Schizophrenia Patients

2016 ◽  
Vol 12 (2) ◽  
pp. 40-43
Author(s):  
Fatema Zerin Khan ◽  
Syeda Papia Sultana ◽  
Mohammad SI Mullick ◽  
Nargis Akhter
Keyword(s):  
Oxidative Stress ◽  
Ascorbic Acid ◽  
Antioxidant Status ◽  
Armed Forces ◽  
Control Group ◽  
Study Group ◽  
Medical College ◽  
Anti Oxidant ◽  
Endogenous Antioxidants ◽  
Medical University

Introduction: Oxidative stress has been assumed to contribute to the pathophysiology of schizophrenia. Increased oxidative stress is the result of either an increased production of free radicals or a depletion of the endogenous antioxidants. Objective: To assess the levels of oxidative stress and antioxidant status in schizophrenia. Materials and Methods: This observational study was carried out in the department of Pharmacology, Bangabandhu Sheikh Mujib Medical University from September 2013 to January 2015. Ninety three schizophrenia patients were enrolled as study group and 30 healthy indivuduals were taken as control group. The peripheral levels of several molecules associated with oxidative stress namely malondialdehyde (MDA), glutathione (GSH) and anti-oxidant status like plasma levels of ascorbic acid (vitamin C) and α-tocopherol (vitamin E) in 93 patients with schizophrenia and 30 healthy participants were assessed. Results: Study found that the schizophrenia group presented substantially higher levels of oxidative stress than the control group, as revealed by elevated quantities of the pro-oxidant MDA (6.3±0.5μmol/L in study group and 2.1±0.5μmol/L in control group), decreased levels of the antioxidants GSH (0.6±0.2mg/gm of Hb in study group and 2.1±0.5mg/gm of Hb in control group), plasma α-tocopherol and ascorbic acid. Results found were highly significant (p=0.001). Conclusion: In schizophrenia there are increased level of oxidative stress and decreased level of the antioxidants. Journal of Armed Forces Medical College Bangladesh Vol.12(2) 2016: 40-43

Serum and peritoneal fluid levels of ischemia modified albumin in moderate/severe endometriosis

2017 ◽  
Vol 6 (9) ◽  
pp. 3721
Author(s):  
Koray Gok ◽  
Yasemin Tasci ◽  
Berna Dilbaz ◽  
Selda Demirtas ◽  
Sedat Ozdemir ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Peritoneal Fluid ◽  
Colorimetric Method ◽  
Control Group ◽  
Controlled Clinical Trial ◽  
Study Group ◽  
Ischemia Modified Albumin ◽  
Median Serum ◽  
And Control

Background: Recently, the role of oxidative stress in progression of endometriosis has been reported. Ischemia-modified albumin (IMA) is a marker of protein oxidation and very limited number of studies has evaluated the role of IMA in endometriosis. This study was designed to evaluate the serum and peritoneal fluid IMA levels in moderate/severe endometriosis as a marker for oxidative stress.Methods: This study was designed as a prospective controlled clinical trial. The study group consisted of 35 cases who underwent laparoscopy and with a diagnosis of moderate/severe endometriosis. The control group (n=35) was cases without endometriosis that underwent laparoscopy for tubal sterilization. The serum and peritoneal fluid IMA levels were measured spectrophotometrically by colorimetric method with complex of albumin non-binding cobalt and dithioerthreitol.Results: Although the median serum IMA levels in study and control groups were similar (p=0.553), the levels of peritoneal fluid IMA were significantly higher in study group (p=0.044). In endometriosis cases with dysmenorrhea peritoneal fluid IMA levels were much higher than cases without dysmenorrhea (p=0.018).Conclusions: The increased levels of IMA in peritoneal fluid of endometriosis support the possible role of oxidative stress in endometriosis. With this study, peritoneal fluid IMA levels are initially documented in endometriosis cases.

Evaluation of serum ischaemia-modified albumin levels in patients with Bell's palsy

2019 ◽  
Vol 133 (09) ◽  
pp. 810-813
Author(s):  
A E Göker ◽  
M H Alagöz ◽  
G Güntaş ◽  
A Baskadem Yilmazer ◽  
G Berkiten ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Bell’S Palsy ◽  
Control Group ◽  
Study Group ◽  
Healthy Individuals ◽  
Bell's Palsy ◽  
Control Groups ◽  
Blood Samples ◽  
And Control ◽  
The Relationship

AbstractObjectiveThis prospective study aimed to evaluate the relationship between serum ischaemia-modified albumin levels and Bell's palsy severity.MethodsThe study included 30 patients diagnosed with Bell's palsy and 30 healthy individuals. The patients were separated into three disease severity groups (grades 2, 3 and 4) according to House–Brackmann classification. Blood samples were collected from all participants and the results compared between groups.ResultsSignificant differences in serum ischaemia-modified albumin were found between the study and control groups (p &lt; 0.001); values were significantly higher in the study group than in the control group.ConclusionThe significantly higher levels of serum ischaemia-modified albumin in the study group suggest that Bell's palsy pathogenesis is associated with oxidative stress.

scholarly journals Safety Measures in Biochemistry Laboratory: Need of the Hour

2020 ◽  
Author(s):  
Ashish Prakash Anjankar ◽  
Swanand Pathak ◽  
Archana Dhok
Keyword(s):  
Medical Students ◽  
Test Scores ◽  
Test Score ◽  
Control Group ◽  
Study Group ◽  
Laboratory Safety ◽  
Learning Gain ◽  
Safety Measures ◽  
Post Test ◽  
And Control

Introduction: Biochemistry laboratories like any other laboratory contain certain inherent dangers and hazards for the students. Proper guidelines regarding laboratory safety are missing in Biochemistry curriculum. So, skill training about laboratory safety in Biochemistry laboratory will be useful for the MBBS students. Aim: To train the undergraduate medical students about laboratory safety using “Safety measures in Biochemistry Laboratory” module and to assess their perception about this module. Materials and Methods: A prospective interventional study was conducted in which 100 students of first year MBBS 2017 batch were included which were divided into study and control groups. In Study Group, 50 students were trained in the topic of laboratory safety using a module on “Safety measures in Biochemistry Laboratory” which included three sessions of didactic lectures and two sessions of hands-on training. In Control Group, 50 students were not given any intervention. Outcome of training was assessed with the help of “Biochemistry Laboratory Safety Index” (BLSI) scores, which was designed by authors and was validated from 10 external subject experts of the Biochemistry Department. Pre-test and post-test marks of study and control group participants were compared using paired t-test for each group. Absolute learning gain (Post-test score-Post-test score) and relative learning gain (Post-test score-Pre-test score/Pre-test score) was calculated. Results: Absolute learning gain for control and study group was 0.11 and 5.53, respectively, while relative learning gain for control and study group was 0.16 and 89.62 respectively. Pre-test and post-test scores are statistically non-significant (p>0.05) in the control group, while Pre-test and post-test scores are statistically significant (p<0.001) in the study group. BLSI scores in control group was excellent for 9 (18%), good for 16 (32%), average for 19 (38%) and poor for 06 (12%) students; while in study group it was excellent for 41 (82%), good for 08 (16%) students, average for 01 (02%) students and poor for nil students. Conclusion: Module of “Safety measures in Biochemistry Laboratory” is significantly effective educational intervention as it improves the knowledge about Laboratory safety and its implication for medical students during Biochemistry practicals. Training about “Safety measures in Biochemistry Laboratory” can be incorporated in the existing undergraduate Curriculum of Biochemistry.

scholarly journals The Effect of Nigella Sativa on Clinical Improvement of Laryngopharyngeal Reflux Patients

2020 ◽  
Vol 1 (2) ◽  
pp. 5-9
Author(s):  
Lusia - Lusia ◽  
Farokah - Farokah ◽  
Rery - Budiarti
Keyword(s):  
Clinical Improvement ◽  
Laryngopharyngeal Reflux ◽  
Nigella Sativa ◽  
Upper Esophageal Sphincter ◽  
Control Group ◽  
Exact Test ◽  
Black Cumin ◽  
Post Test ◽  
Anti Oxidant ◽  
And Control

Background : Laryngopharyngeal Reflux (LPR) decribes as retrograde reflux from gaster into the laryngopharynx because of the premature relaxation upper esophageal sphincter. The management of LPR patients includes lifestyle and dietary arrangements, medical therapy and surgical management. Nigella sativa (NS) / black cumin  has anti-inflammatory, anti-oxidant and gastroprotective effects that are expected to provide clinical improvement in LPR patients.Objective : The purpose of this study was to determine the effectiveness of NS on the clinical improvement of LPR patients.Methods : This research is an intervention study using pre and post test design methods of 34 LPR patients in Dr. Kariadi, hospital and 2 affiliated hospital  from November 2019 - March 2020.Result : 24 patients with complete data while 10 patients were unable to evaluate RFS because of a pandemic. The treatment group (15 patients) were given  omeprazole 20 mg / 12 hours plus NS 1000 mg / 12 hours orally and control group (9 patients) were given omeprazole 20 mg / 12 hours orally and placebo. Data were tested by Shapiro Wilk test, followed by the appropriate parametric test, and Fisher's exact test. There were no differences in clinical improvement in LPR based on RSI and RFS values between groups given additional NS and groups without NS, p 0.105.Conclusion : there were no significant differences in clinical improvement between groups given additional NS and groups without NS Keyword : Laryngopharyngeal Reflux, Reflux Symptoms Index, Reflux Finding Score, Omeprazol, Nigella sativa

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