Formulation of herbal cream based on Ziziphus Mauritiana leaves extract and evaluation on physicochemical properties

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Siti Umairah Mokhtar ◽  
Ashviny Murugan ◽  
Che Syahira Che Awang
Keyword(s):  
Thermal Stability ◽  
Antioxidant Activity ◽  
Methanolic Extract ◽  
Herbal Medicines ◽  
Soxhlet Extraction ◽  
Modern Medicine ◽  
Ziziphus Mauritiana ◽  
Personal Care Product ◽  
Ic50 Value ◽  
Good Homogeneity

Plant derived substances and herbal medicines have recently attracted the great interest towards their versatile application, as medicinal plants used in traditional and modern medicine. The aim of the current study was to investigate the antioxidant activity of Ziziphus mauritiana leaves extract followed with the formulation of herbal creams based on ZM methanolic extract then evaluate the physical characteristic and stability of the creams. After the methanolic extract was obtained by using Soxhlet extraction, the extract was assessed for antioxidant activity by using stable 2,2- Diphenyl-1-picrylhydrazyl (DPPH) which showed the IC50 value of the ZM extract is 10.57 µg/ml, while IC50 value for ascorbic acid is 4.19 µg/ml. Four formulations of water in oil (w/o) emulsion based cream was formulated based on Ziziphus mauritiana leaves extract which are F1, F2, F3 and F4. Several physical properties were evaluated such as organoleptic, pH, viscosity, homogeneity, washability and emmolliency which proved that all formulations have good homogeneity, non greasy and under suitable pH and viscosity. Based on thermal stability test (45 °C ± 70 % RH, for 48 hours), it showed that F4 is not stable at high temperature compared to other formulations. Therefore, the present study indicated that the Ziziphus mauritiana leaves extract has great potential for personal care product development.

scholarly journals Development, characterization and evaluation of poly-herbal ointment and Gel formulation containing Nerium Indicum Mill, Artocarpus Heterophyllus Lam, Murraya Koenigii Linn, Punica Granatum Linn

2019 ◽  
Vol 9 (2) ◽  
pp. 64-69
Author(s):  
Renuka Shukla ◽  
Varsha Kashaw
Keyword(s):  
Herbal Medicines ◽  
Soxhlet Extraction ◽  
Punica Granatum ◽  
Modern Medicine ◽  
Murraya Koenigii ◽  
Artocarpus Heterophyllus ◽  
Ointment Formulation ◽  
Physicochemical Evaluation ◽  
Medicinal Properties ◽  
Gel Formulations

Objective: The objective of present study was the development, characterization and evaluation of poly-herbal ointment and gel formulations of Nerium Indicum Mill, Artocarpus Heterophyllus Lam, Murraya Koenigii Linn, Punica Granatum Linn extract. Plant derived substances and herbal medicines have recently attracted the great interest towards their versatile application. Medicinal plants are the richest source of bioactive compounds used in traditional and modern medicine. The ointment and gel formulation of plant extract has a sound approach, The poly-herbal ointment and gel formulation were evaluated for its physicochemical parameters like color, odour, pH, spreadability, extrudability, consistency, diffusibility, solubility, washability, non-irritancy and stability. Materials and methods: Extract of all four plants has been separated by the soxhlet extraction by 70% ethanol. Poly-herbal ointment and gel formulation has been prepared by mixing the extract of Nerium Indicum Mill, Artocarpus Heterophyllus Lam, Murraya Koenigii Linn, Punica Granatum Linn with carbapol 940 for Gel formulation and PEG 2000 & PEG 600 for ointment formulation. Results and conclusion: Physicochemical evaluation shows that the poly-herbal ointment and gel formulations fulfilled the criteria as herbal formulation and irritancy test also performed to indicate the effectiveness and efficacy of prepared ointment and gel formulations. The poly-herbal ointment and gel formulations were also evaluated for its stability at various temperature conditions which shows no change in the irritancy, spreadability and diffusion study. Thus it could become a medium to use the medicinal properties of extracts effectively and easily as a simple dosage form. Keywords:  Nerium Indicum Mill, Artocarpus Heterophyllus Lam, Murraya Koenigii Linn, Punica Granatum Linn, extract, ointment, gel.     

AKTIVITAS ANTIOKSIDAN DAN FLAVONOID EKSTRAK BIJI KURMA

EKOLOGIA ◽  
2020 ◽  
Vol 19 (1) ◽  
pp. 34-38
Author(s):  
Siti Warnasih ◽  
Diana Widiastuti ◽  
Uswatun Hasanah ◽  
Laksmi Ambarsari ◽  
Purwantiningsih Sugita
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Soxhlet Extraction ◽  
Ethyl Acetate Fraction ◽  
Positive Control ◽  
Palm Fruit ◽  
Ic50 Value ◽  
Dpph Method ◽  
Fruit Processing

Date seeds are waste from palm fruit processing that has not been utilized optimally. Date seeds contain flavonoids which are known to act as antioxidants. The aim of this study was to determine the antioxidant activity and flavonoid of methanol extract which was carried out by soxhletation and its fractionation results. Date seeds are made into simplicia, soxhlet extraction with methanol, then the methanol extract is fractionated in stages by liquid-liquid partition with n-hexane, ethyl acetate, and n-butanol. Each fraction and extract was determined by its antioxidant activity with the DPPH method and its flavonoid was determined by spectrophotometry. Ethyl acetate fraction has the strongest antioxidant activity with the lowest IC50 value, that is equal to 5.74 ± 0.05μg/mL, followed by methanol extract of 9.55±0.53 μg/mL, n-butanol fraction 19.73±0,58 μg/mL, and n-hexane fraction of 289.59±10.52 μg/mL, while the IC50 value for vitamin C as a positive control was 4.29±0.74 μg/mL. The highest flavonoids were produced from ethyl acetate fractions which amounted to 1484.33 ± 161.47 mg Quercetin Equivalent (QE) / 100 g, followed respectively by methanol extract of 282.84±13.72 mg QE/100 g.

scholarly journals α-Amylase Inhibition, Antioxidant Activity and Phytochemical Analysis of Calotropis gigantea (L.) Dryand

2020 ◽  
Vol 10 (1) ◽  
pp. 77-81
Author(s):  
Rajendra Gyawali ◽  
Bijay Bhattarai ◽  
Susan Bajracharya ◽  
Surakshya Bhandari ◽  
Puja Bhetwal ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Inhibitory Activity ◽  
Methanolic Extract ◽  
Ethyl Acetate Fraction ◽  
Gas Chromatography Mass Spectrometry ◽  
Phytochemical Analysis ◽  
Calotropis Gigantea ◽  
Ic50 Value ◽  
Loaded Column ◽  
Chloroform Methanol

Introduction: Antioxidant and α-Amylase inhibitory activity of methanolic extract of Calotropis gigantea (L.) Dryand leaves were evaluated. Methods: The antioxidant activity was evaluated by DPPH assay. The extract was fractionated in Silica gel loaded column chromatography (CC). All fractions were evaluated for their purity by TLC. Out of 11 fractions from CC, one fraction was analyzed by Gas Chromatography-Mass Spectrometry (GC-MS). Results: The antioxidant activity of methanolic extract was found satisfactory (IC50268.80 µg/ml) as compared with ascorbic acid (141.82 µg/ml). TLC of a fractions showed a compound at Rf value at 0.45 in toluene: chloroform: methanol with mobile phase ratio 7:2:1 respectively. Conclusions: Total 17 compounds were identified by GC-MS of ethyl acetate fraction and 5-hydroxyl methyl furfural was major furan compound (59.49%). α-Amylase inhibitory activity of the same fraction showed IC50 value of 0.94 mg/ ml. The Nepalese originated C. gigentea (L.) Dryand possesses antioxidant and α-Amylase inhibitory property.

scholarly journals Ethnopharmacological Study and Evaluation of the Antioxidant Activity of Ocimum basilicum L. Extracts

2020 ◽  
Vol 10 (4-s) ◽  
pp. 132-137
Author(s):  
Hassiba Benabdallah ◽  
Fatima Benchikh ◽  
Smain Amira
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Methanolic Extract ◽  
Radical Scavenging ◽  
Ocimum Basilicum ◽  
Aqueous Extracts ◽  
Antioxidant Power ◽  
Dry Extract ◽  
Mode Of Application ◽  
Ic50 Value

Ocimum basilicum L. (O. basilicum L.) with the vernacular name of Reihan or Reyhan is a medicinal plant from the Lamiaceae family, widely used in traditional Algerian medicine. The aim of the present study was to research the traditional uses and the mode of application of O. basilicum L. in the M’sila region (northern Algeria), to estimate the content of polyphenols and flavonoids in methanolic and aqueous extracts of this plant and to evaluate the antioxidant activity of these extracts by the 2, 2’-diphenyl-1-picryl hydrazyl (DPPH). The results of ethnopharmacological survey showed that decoction of leaves in fresh or dry form is the most frequent use. The yields of extracts were 28.67% and 15.24% for the methanolic and aqueous extracts, respectively. The phytochemical screening revealed the presence of different compounds such as polyphenols, flavonoids, tannins and essential oils. The results demonstrated that the methanolic extract presented a high polyphenols and flavonoids contents with 225.99 ± 3.13 mg gallic acid equivalent/g dry extract and 83.63 ± 3.48 mg quercetin equivalent/g dry extract, respectively. The evaluation of the antioxidant power is carried out using the method of trapping of the free radical DPPH, with BHT as reference antioxidant. The results showed that the methanolic extract has a significant free radical scavenging capacity with an IC50 value of 304.82 ± 24.15 µg/ml; which was greater than the trapping capacity of BHT (IC50 = 327.46 ± 13.11 µg/ml). Whereas, the aqueous extract has a weaker effect with an IC50 value of 2122.81 ± 107.77 µg/ml. Keywords: BHT, DPPH, flavonoids, Ocimum basilicum L., polyphenols.

scholarly journals Development, characterization and evaluation of herbal ointment containing momordica charantia, pongamia glabra and piper nigrum

2019 ◽  
Vol 9 (1) ◽  
pp. 136-140
Author(s):  
Rashmi Shukla ◽  
Varsha Kashaw
Keyword(s):  
Herbal Medicines ◽  
Soxhlet Extraction ◽  
Modern Medicine ◽  
Momordica Charantia ◽  
Piper Nigrum ◽  
Pongamia Glabra ◽  
Ointment Formulation ◽  
Physicochemical Evaluation ◽  
Medicinal Properties ◽  
And Diffusion

Objective: The objective of present study was the development, characterization and evaluation of poly-herbal ointment formulation of pongamia glabra, piper nigrum and momordica charantia extract. Plant derived substances and herbal medicines have recently attracted the great interest towards their versatile application. Medicinal plants are the richest source of bioactive compounds used in traditional and modern medicine. The ointment formulation of plant extract has a sound approach to develop a poly-herbal ointment. The poly-herbal ointment formulation was evaluated for its physicochemical parameters like color, odor, pH, spreadability, extrudability, consistency, diffusibility, solubility, washability and stability. Materials and methods: Extract of all three plants has been separated by the soxhlet extraction. Poly-herbal ointment has been prepared by mixing the extract of momordica charantia, pongamia glabra and piper nigrum with wool fat, Cetostearyl alcohol and paraffin. Results and conclusion: Physicochemical evaluation shows that the poly-herbal ointment fulfilled the criteria as herbal formulation and irritancy test also performed indicate the effectiveness and efficacy of prepared ointment formulations. The poly-herbal ointment formulations were also evaluated for its stability at various temperature conditions which shows no change in the irritancy, spreadability and diffusion study. Thus it could become a medium to use the medicinal properties of extracts effectively and easily as a simple dosage form. Keywords: Momordica charantia, pongamia glabra, piper nigrum, ointment, Cetostearyl alcohol.

scholarly journals Physicochemical characteristics, thermal stability and antioxidant characteristics of Trichosanthes kirilowii maxim seed oil as affected by different extraction methods

2017 ◽  
Vol 68 (3) ◽  
pp. 206
Author(s):  
Z. K. Hou ◽  
Q. Z. Ji ◽  
L. Yang ◽  
Z. Q. Gao ◽  
B. C. Wang
Keyword(s):  
Thermal Stability ◽  
Antioxidant Activity ◽  
Unsaturated Fatty Acids ◽  
Oil Quality ◽  
Radical Scavenging ◽  
Soxhlet Extraction ◽  
Physicochemical Characteristics ◽  
Extraction Methods ◽  
Punicic Acid ◽  
Trichosanthes Kirilowii

In conducting this study, the extraction of Trichosanthes kirilowii maxim seed oils (TSO) was carried out with the help of cold pressing (CP), hot pressing (HP) and soxhlet extraction (SE). Investigation, together with comparison, was carried out with respect to the physicochemical properties, thermal stability and antioxidant action of TSO. The key ingredients in the seeds consisted of fat, fiber and protein. The physicochemical characteristics of the oils brought to light the fact that CPTSO possessed top oil quality. The findings also suggested that linoleic acid, punicic acid and oleic acid were the leading unsaturated fatty acids in TSO. It was also discovered that TSO had an almost identical chemical composition regardless of the extraction method was used. It was demonstrated by TG/DTG curves that both HPTSO and CPTSO had more thermal stability in comparison with SETSO. Furthermore, the antioxidant activity assessments emphasized that CPTSO had better radical scavenging potential. CP had the ability to deliver an extract with higher quality as well as antioxidant activity in comparison with HP and SE methods and can be taken into consideration as a more suitable method in order to attain high quality oil.

scholarly journals Antilipase and antioxidant activity of Orthosiphon stamineus methanolic extract

2016 ◽  
Vol 15 (1) ◽  
Author(s):  
Norsyuhada Alias ◽  
Adam Leow Thean Chor ◽  
Mohd. Shukuri Mohamad Ali ◽  
Abu Bakar Salleh ◽  
Asilah Ahmad Tajudin ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Inhibitory Activity ◽  
Methanolic Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Orthosiphon Stamineus ◽  
Ic50 Value ◽  
High Antioxidant Activity ◽  
Treatment Of Obesity ◽  
Hydrolysis Of

Introduction: Antilipase from natural resources are a potential tool for the treatment of obesity while antioxidant-rich plants are essential in combating degenerative diseases. The aim of this study is to determine the antilipase and antioxidant activity of Orthosiphon stamineus methanolic extract. Methods: The inhibitory activity against pancreatic lipase was determined by measuring the hydrolysis of p-nitrophenyl butyrate to p-nitrophenol at 405 nm. Antioxidant activity of O. stamineus extract was measured by 2, 2, diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity assay. Results: The O. stamineus crude extract exhibited strong lipase inhibitory activity with an IC50 value of 34.7 µg/ml. The inhibition mode study disclosed that O. stamineus could act as uncompetitive inhibitor. O. stamineus showed high antioxidant activity with an EC50 value of 26.3 µg/ml. Conclusions: The results suggest that O. stamineus has shown potential as a source of natural antilipase and antioxidant.

scholarly journals Aktivitas Antioksidan dan Antimikroba Minyak Atsiri Kembang Leson

2020 ◽  
Vol 13 (2) ◽  
pp. 139-146
Author(s):  
Ambar Pratiwi ◽  
Inas Salimah
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Inhibitory Concentration ◽  
Herbal Medicines ◽  
Diffusion Method ◽  
E Coli ◽  
Ic50 Value ◽  
Dpph Method

AbstrakKembang leson adalah racikan jamu untuk mandi yang terdiri dari berbagai obat-obatan herbal yang umum ditemukan di Jawa. Kembang leson mengandung minyak atsiri utama, yaitu camphene 1,29%, benzene methyl cymene 4,93%, camphor 4,75%, cyclohexane methanol 7,56%, dan curdione 4,83%. Golongan senyawa minyak atsiri dapat menghambat radikal bebas 2,2-diphenyl-1-picrylhydrazyl (DPPH) dan memiliki aktivitas antibakteri yang ditunjukkan dengan uji aktivitas antibakteri terhadap Staphylococcus aureus dan Escherichia coli. Tujuan penelitian ini adalah untuk mengetahui aktivitas antioksidan dan antibakteri dari minyak atsiri kembang leson. Minyak Atsiri kembang leson diekstraksi dengan metode destilasi. Minyak atsiri yang diperoleh diuji aktivitas antioksidan dengan metode DPPH, serta aktivitas antibakteri dengan metode difusi. Hasil penelitian menunjukkan bahwa minyak atsiri kembang leson memiliki aktivitas antioksidan dengan persentase penghambatan DPPH sebesar 56,16%, dan memiliki nilai IC50 (half maximal inhibitory concentration) sebesar 825,78 ppm. Aktivitas antibakteri minyak atsiri kembang leson juga efektif menghambat pertumbuhan E. coli, tetapi belum mampu menghambat pertumbuhan S. aureus. Minyak atsiri kembang leson  dapat digunakan sebagai sumber antioksidan alami. Abstract Kembang leson is an herbal concoction used for bathing. It comprises various herbal medicines found in Java. Kembang leson contains essential oils such as camphene 1.29%, benzene methyl cymene 4.93%, camphor 4.75%, cyclohexane methanol 7.56% and curdione 4.83%. It is known that essential oils can reduce DPPH free radicals and have antibacterial activity against S. aureus and E. coli.  Our research to determine the antioxidant and antibacterial activity of kembang leson essential oil. The essential oil was extracted by distillation. The essential oil obtained was tested for antioxidant activity using the DPPH method, and antibacterial activity using the diffusion method. The results show that kembang leson essential oils have antioxidant and antibacterial activity. The percentage of DPPH inhibition was 56.16% and the IC50 value was 825.78 ppm. Antibacterial activity of kembang leson essential oil inhibited the growth of E. coli but could not inhibit the growth of S. aureus. Thus, kembang leson essential oils have antioxidant and antibacterial activity against E. coli, but only have antioxidant activity against S. aureus. Further studies are needed to determent the main ingredients that play an important role in the mechanism of antioxidants and antibacterial of kembang leson essential oil.

scholarly journals In –vitro Antioxidant Activity of Methanolic Extract of the Roots of Bergenia ciliata

2018 ◽  
Vol 1 (4) ◽  
pp. 31-34
Author(s):  
Suman Lal Shrestha ◽  
Suresh Awale ◽  
Surya Kant Kalauni
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Methanolic Extract ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Ic50 Value ◽  
Free Radical Scavenging Assay ◽  
Radical Scavenging Assay ◽  
In Vitro Antioxidant Activity

Bergenia ciliata is an essential medicinal plant used in regions where western medicines are inaccessible due to their unavailability and high cost. The methanolic extract of Bergenia ciliataroots was separated for phytochemical elements and in-vitro antioxidant activity. The plant extract showed the rich outgrowth of secondary metabolites that play the role for biological activities. The higher antioxidant functioning of the plant is due to the occurrence of reactive elements like phenols and flavonoids. The antioxidant functioning of the plant extract was measured by DPPH free radical scavenging assay. In DPPH free radical scavenging assay the IC50 value of Bergenia ciliata was found to be 11.21μg/mL, while the IC50 value of standard ascorbic acid was found to be 45.93μg/mL

scholarly journals In-vitro Cytotoxicity and Antioxidant Studies of Elaeocarpus serratus

1970 ◽  
Vol 2 (2) ◽  
pp. 86-90 ◽  
Author(s):  
Most Nazma Parvin ◽  
Shammy Sarwar ◽  
Sadia Afreen Chowdhury ◽  
Hasan Mohammed Zakaria ◽  
Naz Hasan Huda
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Carbon Tetrachloride ◽  
Methanolic Extract ◽  
Soluble Fraction ◽  
Reducing Power ◽  
Stem Bark ◽  
Scavenging Activity ◽  
Ic50 Value

The pet-ether soluble fraction (PEF), Carbon tetrachloride soluble fraction (CTSF) and Chloroform soluble fraction (CFSF) of the methanolic extract of  stem bark of Elaeocarpus serratus were investigated for cytotoxic activity using brine shrimp lethality bioassay .The methanolic extract was also evaluated for possible antioxidant activity using nitric oxide scavenging activity and reducing power assays. In Elaeocarpus serratus, the LC50 value were found to be 14.94, 0.831 & 3.288 μg/mL in pet-ether soluble fraction (PEF), Carbon tetrachloride soluble fraction (CTSF) and Chloroform soluble fraction (CFSF) of the methanolic extract respectively. The extract showed significant antioxidant activity in nitric oxide scavenging activity and reducing power assay. In nitric oxide scavenging activity, the IC50 value of Elaeocarpus serratus extract was 89.325μg/mL while the IC50 value of ascorbic acid was 47.684μg/mL. It was found that scavenging of nitric oxide by the extract was concentration dependent.  Key words: Elaeocarpus serratus; Cytotoxicity; Antioxidant; Reducing power.DOI: 10.3329/sjps.v2i2.5829Stamford Journal of Pharmaceutical Sciences Vol.2(2) 2009: 86-90

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