Effect of Sleep Restriction on Cardiometabolic and Haemoinflammatory Parameters in Adult Male Wistar Rats

Background and Objective: While insufficient sleep remains an under-recognized public health issue across the globe, there is paucity and heterogeneity of data regarding its cardiometabolic and haemoinflammatory implications. We, therefore, aimed to evaluate the impact of chronic sleep restriction on cardiometabolic and haemoinflammatory parameters in rats. Materials and Methods: 16 male Wistar rats (aged 8-10 weeks) were randomly assigned into equal control or sleep restriction groups. Gentle handling was used to induce sleep restriction for six weeks. Fasting weight and blood sugar were obtained and lipids were analyzed using their respective Randox kits. Malondialdehyde (MDA) levels and catalase (CAT) and superoxide dismutase (SOD) activities were assayed. Full blood count and CD4+ T cell count were determined using automated analyzer. Data were analyzed using Student’s t-test, with level of significance set at P ≤ 0.05, via SPSS software. Results: Chronic sleep restriction caused significant initial weight loss, increase in feed consumption, and percentage increase in fasting blood sugar (FBS) (32% vs. 15%). We also noted the triglyceride-glucose (TyG) index of sleep-restricted rats to be significantly higher (6.22) than that of controls (5.62). In addition, a significant reduction in monocyte count, monocyte-lymphocyte ratio (MLR), and absolute CD4+ cell count among the sleep-restricted rats was observed. Conclusion: Our findings have provided objective evidence that, over the course of 6 weeks, 5 hours of sleep restriction had caused body weight gain, hyperglycaemia, insulin resistance, and impairment in immunoinflammatory status; hence, it could be a risk factor for developing cardiometabolic syndrome and immune-related disorders.

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Anti-malaria and hypoglycaemic activities of Diosgenin on alloxan-induced, diabetic Wistar rats

GSC Biological and Pharmaceutical Sciences ◽

10.30574/gscbps.2021.15.3.0102 ◽

2021 ◽

Vol 15 (3) ◽

pp. 073-079

Author(s):

Omoirri Moses Aziakpono ◽

Madubogwu Ngozi Ukamaka ◽

Oraekei Daniel Ikechukwu ◽

Ataihire Johnson Uyovwiesevwa ◽

Chukwuemeka Charles Ofili ◽

...

Keyword(s):

Body Weight ◽

Blood Sugar ◽

Wistar Rats ◽

World Health ◽

Treatment Groups ◽

Male Wistar Rats ◽

Normal Rat ◽

Sugar Levels ◽

Health Organization ◽

Alloxan Monohydrate

The rising threat of Plasmodium falciparum resistance to Monotherapies has prompted the world health organization (WHO) 2006 guidelines to recommend the use of different anti-malarias. In this study, the anti-malaria and hypoglycaemic activities of Diosgenin, a potent, yet poorly reported saponin was investigated on P. falciparum inoculated and Alloxan-Induced, Diabetic Wistar Rats. Fort two (42) adult male wistar rats of between 100g and 150g were procured, acclimatized (for two weeks), and grouped into seven of six (6) rats per group. While Group 1 (Normal control) received normal rat chow and water ad libitum, groups 2 – 4 received no treatment (untreated), 10 mg/kg body weight of anti-diabetic Metformin and 25 mg/kg body weight of diosgenin respectively after inducing diabetes mellitus (DM) with alloxan monohydrate; whereas, groups 5-7 (all malaria infected) were untreated (negative malaria control), 25 mg/kg body weight of diosgenin and 56 mg/kg body weight of anti-malaria coartem respectively. Following treatment period, blood samples were obtained and assayed for fasting blood sugar, packed cell volume (PCV) and total white blood cell count (TWBCC). From the result, P. falciparum exposed rats showed lowered PCV values than control with observed improvements in coartem (significant at p < 0.05) and diosgenin (insignificant) treatment groups. Also, diabetic, diosgenin treated rats showed an insignificant reduction in blood sugar levels compared to control, even though this change was apparently improved compared to diabetic, untreated group. Again, TWBCC caused notable decrease in diosgenin treated, though this decrease signified a huge recovery compared to untreated rats. Corroborative studies on diosgenin with other systems is recommended.

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Safety Evaluation of Crude Ethanol Extract of Waltheria Indica Linn. Root In Male Wistar Rats

Journal of Veterinary and Biomedical Sciences ◽

10.36108/jvbs/1202.30.0131 ◽

2021 ◽

Vol 3 (1) ◽

pp. 112-125

Author(s):

A. Basiru

Keyword(s):

Wistar Rats ◽

Safety Evaluation ◽

Ethanol Extract ◽

Serum Levels ◽

Hepatic Necrosis ◽

Control Group ◽

Percentage Increase ◽

Male Wistar Rats ◽

Group A ◽

Renal Tubular

Safety evaluation of Waltheria indica Linn. root was carried out in Wistar rats. Twenty adult male Wistar rats were randomly divided into four groups (n=5); A–D. Group A served as control group while groups B, C and D were administered with 200, 400 and 800 mg/Kg of crude ethanol extract of Waltheria indica Linn. root. After 28 days of administration, the rats were sacrificed and blood samples were collected and serum prepared. Haematological and biochemical parameters, as well as serum electrolyte were determined according to standard procedures. The 200 mg/Kg of Waltheria indica Linn. root caused the highest percentage increase in body weight. The kidney was also significantly increased at 400 mg/kg. The total WBC and lymphocytes counts were significantly increased at 400 mg/kg. The serum levels of ALT and chloride were significantly increased at 800 mg/kg. Waltheria indica Linn. root also caused hepatic necrosis and renal tubular degeneration in Wistar rats. Therefore, Waltheria indica Linn. root should be used with caution especially at higher dose despite its therapeutic potentials.

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Marine n-3 fatty acids promote size reduction of visceral adipose depots, without altering body weight and composition, in male Wistar rats fed a high-fat diet

British Journal Of Nutrition ◽

10.1017/s0007114509353210 ◽

2009 ◽

Vol 102 (7) ◽

pp. 995-1006 ◽

Cited By ~ 40

Author(s):

Merethe H. Rokling-Andersen ◽

Arild C. Rustan ◽

Andreas J. Wensaas ◽

Olav Kaalhus ◽

Hege Wergedahl ◽

...

Keyword(s):

Fatty Acids ◽

Mrna Expression ◽

High Fat Diet ◽

Wistar Rats ◽

Plasma Lipid ◽

Feed Consumption ◽

High Fat ◽

Male Wistar Rats ◽

Similar Body ◽

Visceral Adipose

We evaluated the effects of partly substituting lard with marine n-3 fatty acids (FA) on body composition and weight, adipose tissue distribution and gene expression in five adipose depots of male Wistar rats fed a high-fat diet. Rats were fed diets including lard (19·5 % lard) or n-3 FA (9·1 % lard and 10·4 % Triomar™) for 7 weeks. Feed consumption and weight gain were similar, whereas plasma lipid concentrations were lower in the n-3 FA group. Magnetic resonance imaging revealed smaller visceral (mesenteric, perirenal and epididymal) adipose depots in the n-3 FA-fed animals (35, 44 and 32 % reductions, respectively). n-3 FA feeding increased mRNA expression of cytokines as well as chemokines in several adipose depots. Expression of Adipoq and Pparg was enhanced in the mesenteric adipose depots of the n-3 FA-fed rats, and fasting plasma insulin levels were lowered. Expression of the lipogenic enzymes Acaca and Fasn was increased in the visceral adipose depots, whereas Dgat1 was reduced in the perirenal and epididymal depots. Cpt2 mRNA expression was almost doubled in the mesenteric depot and liver. Carcass analyses showed similar body fat (%) in the two feeding groups, indicating that n-3 FA feeding led to redistribution of fat away from the visceral compartment.

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ACTIVITY OF ETHYL ACETATE EXTRACT FROM Chrysophyllum cainito L. LEAVES IN DECREASING BLOOD SUGAR LEVEL IN MALE WISTAR RATS

Journal of Islamic Pharmacy ◽

10.18860/jip.v3i1.4996 ◽

2018 ◽

Vol 3 (1) ◽

pp. 31 ◽

Cited By ~ 1

Author(s):

Imam Malikul Hadi Arrijal ◽

Burhan Ma'arif ◽

Arief Suryadinata

Keyword(s):

Ethyl Acetate ◽

Blood Sugar ◽

Blood Level ◽

Blood Sugar Level ◽

Wistar Rats ◽

Ethyl Acetate Extract ◽

Negative Control ◽

Treatment Groups ◽

Male Wistar Rats ◽

Chrysophyllum Cainito

The high morbidity or mortality due to diabetes mellitus becomes the world health problem. That fact encourages the study about antidiabetes drugs from natural material especially plants. This study aims to analyze the effect of ethyl acetate extract from Chrysophyllum cainito L. leaves in decreasing of blood sugar level of alloxan-induced male male wistar rats. This research is considered as experimental research with pre and post test randomized controlled group design. The experimental animals in this study were 25 male male wistar rats divided into 5 treatment groups, treatment with CMC-Na (negative control), metformin with the dose of 9 mg/200gBW (positive control), and leaves extract C. cainito dose 25; 50; 75 mg/kgBW. All treatment groups had significant decrease of blood sugar levels at 7, 10 and 14 day observations with p <0.05. While in the day 3 had insignificant decrease of blood sugar level with p >0.05. Every C. cainito leaves extract doses group have the ability to decrease sugar blood level better than negative control, but still below positif control. The optimum dose in decreasing sugar blood level in this study is 75 mg/kg BW.

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ASTAXANTHIN MENGHAMBAT PENURUNAN JUMLAH SEL LEYDIG DAN KADAR TESTOSTERON PADA TIKUS WISTAR JANTAN YANG DIPAPAR DENGAN ASAP ROKOK

IJCNP (INDONESIAN JOURNAL OF CLINICAL NUTRITION PHYSICIAN) ◽

10.54773/ijcnp.v3i1.42 ◽

2020 ◽

Vol 3 (1) ◽

pp. 56-64

Author(s):

Andrew Lie ◽

Wimpie Pangkahila ◽

L.P. Iin Indrayani Maker

Keyword(s):

Cell Count ◽

Cigarette Smoke ◽

Leydig Cell ◽

Testosterone Level ◽

Leydig Cells ◽

Wistar Rats ◽

Control Group ◽

Study Group ◽

Significant Difference ◽

Male Wistar Rats

Background. The exposure of cigarette smoke impacts negatively on Leydig cell count and testosterone level. Astaxanthin is known for its ability to neutralize free radicals far more potent than any other kind of antioxidant. Research objectives. This research aims to prove the effect of Astaxanthin in inhibiting decrease of Leydig cell count and testosterone level in male Wistar rats exposed with cigarette smoke. Methodology. The posttest only control group study design was conducted on 36 male Wistar rats, 12-16 weeks in age, with 200-210 grams body weight. Samples were randomly divided into two groups, consisting of a control group exposed with cigarette smoke and 0.5 ml of aquadest and a study group exposed with cigarette smoke and 0.1 mg of astaxanthin/200 gr BW daily for 30 days. On day 31, blood samples were taken to measure the testosterone level. Both testes were taken for Leydig cells count assessment. Comparative analysis was done to see any significant difference between the study and control group. Research results. The results show that the mean number of Leydig cells and testosterone levels in the study group was significantly higher than the control group (p<0.01). Conclusion. Oral astaxanthin administration inhibited the decrease of Leydig cell count and testosterone level in male Wistar rats exposed with cigarette smoke. Key words: Astaxanthin, Testosterone, Leydig Cell, Andropause

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Performance characteristics of male wistar rats fed graded levels of stored powdered Corchorus olitorius

International Journal of Scientific Reports ◽

10.18203/issn.2454-2156.intjscirep20170354 ◽

2017 ◽

Vol 3 (2) ◽

pp. 28

Author(s):

Ogungbemi Kunle ◽

S. E. Atawodi ◽

Ishola D. Taiwo ◽

Ishola O. Tomilayo ◽

Ilesanmi F. Funmilayo ◽

...

Keyword(s):

Growth Performance ◽

Feed Intake ◽

Weight Change ◽

Feed Efficiency ◽

Wistar Rats ◽

Feed Consumption ◽

Corchorus Olitorius ◽

Efficient Utilization ◽

Treatment Groups ◽

Male Wistar Rats

Background: This study was carried out to determine the growth performance of rats fed graded levels of Corchorus olitorius supplemented diet.Methods: Twenty male wistar rats weighing between 70g and 90g were purchased and randomly allocated into four treatment groups with graded levels of Corchorus olitorius ( 2.5%, 5% and 10%) while the control had no Corchorus olitorius. The feed intakes as well as weight change of the experimental rats were monitored for eight weeks. Results: The results obtained showed that the highest level of weekly feed intake and feed efficiency were recorded for animals in group fed 10% level of Corchorus olitorius supplementation when compared to other treatment groups. Similar result was observed for the weight change which can be attributed to the efficient utilization of feed consumption.Conclusions: Therefore, it can be concluded that leaves of Corchorus olitorius are palatable and highly nutritious and can improve growth performance of rats when fed for eight weeks.

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ANTIHIPERGLIKEMIK EKSTRAK TUMBUHAN SURUHAN (Peperomia pellucida [L.] Kunth) TERHADAP TIKUS WISTAR (Rattus norvegicus L.) YANG DIINDUKSI SUKROSA

JURNAL ILMIAH SAINS ◽

10.35799/jis.13.2.2013.3055 ◽

2013 ◽

Vol 13 (2) ◽

pp. 116 ◽

Cited By ~ 1

Author(s):

Nafila Salma ◽

Jessy Paendong ◽

Lidya I Momuat ◽

Sariyana Togubu

Keyword(s):

Blood Glucose ◽

Treatment Group ◽

Blood Sugar ◽

Plant Extract ◽

Rattus Norvegicus ◽

Wistar Rats ◽

Negative Control ◽

Glucose Levels ◽

Blood Glucose Levels ◽

Male Wistar Rats

ANTIHIPERGLIKEMIK EKSTRAK TUMBUHAN SURUHAN (Peperomia pellucida [L.] Kunth) TERHADAP TIKUS WISTAR (Rattus norvegicus L.) YANG DIINDUKSI SUKROSAABSTRAK Telah dilakukan penelitian untuk menentukan dosis optimum dari ekstrak tumbuhan suruhan (Peperomia pellucida [L.] Kunth) sebagai antihiperglikemik tikus wistar yang diinduksi sukrosa. Penelitian ini menggunakan metode uji toleransi glukosa. Digunakan tikus putih jantan wistar berjumlah 20 ekor tikus yang dibagi dalam 5 kelompok perlakuan yaitu kelompok perlakuan kontrol negatif (K-) diberi CMC 0,5%, kelompok perlakuan kontrol positif (K+) diberi Glibenklamid dosis 0,45 mg/kgBB, kelompok perlakuan ekstrak tumbuhan suruhan dosis 20; 40; 80 mg/kgBB. Data diperoleh dari pemeriksaan kadar gula darah puasa (basal), 30 menit setelah diindukasi sukrosa dan pada menit ke 30, 60, dan 120 setelah diinduksi sediaan per oral. Data diolah dengan uji ANOVA dan Ducan untuk membandingkan antara kelompok kontrol negatif, ekstrak dan kontrol positif. Data hasil pengujian kadar glukosa darah dengan dosis 20; 40; 80 mg/kgBB memberikan penurunan kadar glukosa darah. Pemberian ekstrak tumbuhan suruhan dengan dosis 40 mg/kgBB pada menit ke-120 memberikan hasil yang signifikan dan bebeda nyata dibandingkan kelompok kontrol negatif (CMC 0,5%) dan tidak berbeda nyata dengan glibenklamid dosis 0,45 mg/kgBB. Kesimpulannya pemberian ekstrak tumbuhan suruhan dosis 40 mg/kgBB pada menit ke 120 memberikan penurunan kadar glukosa darah yang efektif dan berbeda nyata (signifikan) dibandingkan pemberian ekstrak tumbuhan suruhan dosis 20 dan 80 mg/kgBB. Kata Kunci : Peperomia pellucida [L.] Kunth, Tumbuhan Suruhan, Kadar Glukosa Darah, Sukrosa. ANTIHYPERGLYCEMIC FROM SURUHAN PLANT (Peperomia pellucida [L.] Kunth) EXTRACT AGAINST WISTAR RATS (Rattus norvegicus L.) WITH INCLUDED BY SUCROSE ABSTRACT The purpose of this research is to determine optimum dose of Suruhan (Peperomia pellucida [L.] Kunth) extract as Antihyperglycemic agent against Wistar Rats with Induced by Sucrose. This research using glucose tolerance test methods. Used 20 male Wistar rats were divided into 5 groups, they are the negative control treatment group (K-) were given 0.5% CMC, the positive control groups (K+) given 0,45 mg/kgBB Glibenklamid, and Suruhan extract treatment group of 20; 40; 80 mg/kgBB dose. Data obtained from the examination offasting blood sugar (basal) content, 30 minutes after induced bysucroseandat minute of 30, 60, and120 after-induced oraldosage.The data were processed by ANAVA and LSD testtocompare betweenthe negativecontrol group, the extractandpositive control. Dataresults of testing blood sugar levels with doses of 20, 40, 80 mg/kgBB gavea decreasein blood glucose levels were significant and significantly different than the negative control group (CMC 0.5%) and did not give a clear difference with 0.45 mg/kgBB dose of Glibenclamide. Agents of plant extract at a dose of 40 mg/kgBB body weight gives better results to the decline in blood glucose levels compared with doses of 20 and 80 mg/kgBB. In conclusion, Suruhan plant extract has an effect on blood glucose levels decreased against white male Wistar rats were induced sucrose. Keywords : Peperomia pellucida [L.] Kunth, Suruhan Plant, Blood Glucose Levels, Sucrose

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Effect of Cinnamon (Cinnamomum cassia) on Blood Sugar, Lipid Profile and Liver Function of Male Wistar Rats

Pakistan Journal of Nutrition ◽

10.3923/pjn.2019.989.996 ◽

2019 ◽

Vol 18 (10) ◽

pp. 989-996

Author(s):

B.K. Adeoye ◽

Z.O. Alonge ◽

M.D. Olumide ◽

I.F. Ani ◽

M.F. Olanrewaju ◽

...

Keyword(s):

Liver Function ◽

Lipid Profile ◽

Blood Sugar ◽

Wistar Rats ◽

Cinnamomum Cassia ◽

Male Wistar Rats

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Effects of Cnidoscolus aconitifolius on Some Hematological Parameters of Male Wistar Rats

Asian Journal of Biotechnology and Bioresource Technology ◽

10.9734/ajb2t/2020/v6i330080 ◽

2020 ◽

pp. 1-5

Author(s):

Ijeoma Ezebuiro ◽

Chibuike Obiandu ◽

Friday Saronee ◽

Ikechukwu I. Weleh ◽

Adesua C. Obiandu

Keyword(s):

Platelet Count ◽

Blood Cell ◽

Cell Count ◽

Wistar Rats ◽

Leaf Extract ◽

Hematological Parameters ◽

Blood Cell Count ◽

Cnidoscolus Aconitifolius ◽

Male Wistar Rats ◽

Red Blood Cell Count

Introduction: Medicinal plants have become increasingly useful as a form of alternative therapy. Cnidoscolus aconitifolius is a medicinal plant applied in folklore remedies in the treatment and prevention of various diseases. Aim: The aim of this study is to assess the effects of Cnidoscolus aconitifolius on some hematological parameters of male Wistar rats. Methodology: A total of 15 male Wistar rats weighing between 100-250 g were randomly placed in groups. Group 1 served as control and received distilled water only; group 2 received 200 mg/kg and group 3 received 400 mg/kg of the hydromethanolic leaf extract of Cnidoscolus aconitifolius. Extract was administered once daily using oro-gastric cannula for 30 days. Blood samples were collected by direct cardiac puncture into appropriate sample tubes for estimation of hematological parameters including red blood cell count (RBC), packed cell volume (PCV), haemoglobin (Hb) concentration, white blood cell count (WBC) and platelet count. Results: Results showed a significant (P<0.05) increase in RBC, PCV, Hb and platelet count with the higher dose of 400 mg/kg body weight of the extract compared to control group. However, the WBC count was not significantly (P>0.05) altered. Conclusion: Oral administration of the leaf extract of Cnidoscolus aconitifolius increases red blood cell count, packed cell volume, hemoglobin level and platelet count at 400 mg/kg body weight.

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Reproductive and Fertility Effects of an Extract of Andrographis paniculata in Male Wistar Rats

International Journal of Toxicology ◽

10.1177/1091581809339631 ◽

2009 ◽

Vol 28 (4) ◽

pp. 308-317 ◽

Cited By ~ 15

Author(s):

J. Joshua Allan ◽

Mukul P. Pore ◽

M. Deepak ◽

B. Murali ◽

Anand S. Mayachari ◽

...

Keyword(s):

Body Weight ◽

Wistar Rats ◽

Sperm Count ◽

Body Weight Gain ◽

The Body ◽

Andrographis Paniculata ◽

Feed Consumption ◽

Adverse Effect Level ◽

Male Wistar Rats ◽

Effect Level

The possible effect of extract of Andrographis paniculata Nees ( A paniculata) standardized to ≥10% andrographolide, the main bioactive component, on male fertility in albino Wistar rats was evaluated, by orally administering 0, 20, 200, and 1000 mg/kg of body weight per day, for 65 days prior to mating and 21 days during mating. The treated groups showed no signs of dose-dependent toxicity. The body weight gain and feed consumption were not affected at any of the dose levels. The testosterone levels and fertility indices in treatment groups were found to be comparable with that of the control indicating no effect on fertility. Total sperm count and sperm motility were not affected. The testes and epididymides did not show any gross and histopathological changes. Based on these findings, it can be concluded that the no-observed adverse effect level of extract of A paniculata (≥10% andrographolide) was found to be more than 1000 mg/kg per day.

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