scholarly journals Effect of Supplemental Chromium, Vitamin E and Selenium on Biochemical and Physiological Parameters of Holstein Friesian Calves under Heat Stress

2021 ◽  
Author(s):  
J. Razia Sultana ◽  
A. Sarat Chandra ◽  
D.B.V. Ramana ◽  
T. Raghunandan ◽  
M. Gnana Prakash ◽  
...  
Keyword(s):  
Heat Stress ◽  
Vitamin E ◽  
Dietary Treatment ◽  
Basal Diet ◽  
Ambient Air ◽  
Physiological Parameters ◽  
Farm Animals ◽  
Control Group ◽  
Albumin Concentration ◽  
Chromium Propionate

Background: Heat stress has been a major concern in tropical, sub-tropical and arid areas affecting the performance of farm animals. Along with vitamin E and Selenium, chromium is gaining importance in combating heat stress. Methods: Twenty-four calves of 7- 8 months old with an average body weight of 172.79±4.39 kg were distributed randomly into four dietary treatment groups (T0, T1, T2 and T3). The calves of control group (T0) were fed on basal diet i.e. total mixed ration (TMR). The basal diet of T1, T2 and T3 on per kg DM basis were supplemented vitamin E (500 IU) + selenium (0.3 mg), chromium propionate (0.5 mg) and chromium propionate (0.5 mg) + vitamin E (500 IU) + selenium (0.3 mg), respectively. Ambient air temperature and humidity were measured for calculation of THI. Blood samples were collected fortnightly and the rectal temperature (RT), respiration rate (RR) and pulse rate (PR) of all the experimental animals were recorded twice daily to study the biochemical and physiological parameters. Result: Supplementation of chromium along with vitamin E and selenium decreased significantly (P less than 0.05) the cortisol levels and increased the albumin concentration. Chromium supplemented groups (T2 and T3) showed greater (P less than 0.05) increase in mean total protein concentration. A significant reduction in RT was observed in all the supplemented groups compared to control. Whereas, the PR and RR differed significantly in T3 group compared to control.

Impacts of feeding zinc-methionine or chromium-methionine on performance, antioxidant status and physiological responses to transportation stress on lambs

2020 ◽  
Vol 60 (6) ◽  
pp. 796
Author(s):  
Soheilla Kaki Soumar ◽  
Fardin Hozhabri ◽  
Mohammad Mehdi Moeini ◽  
Zahra Nikousefat
Keyword(s):  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Basal Diet ◽  
Farm Animals ◽  
Control Group ◽  
Albumin Concentration ◽  
Road Transportation ◽  
Zinc Methionine ◽  
Transportation Stress ◽  
Chromium Methionine

Context Road transportation of farm animals is an unavoidable activity in animal husbandry, which may lead to the stress. Metabolic modifiers, such as minerals, may be an effective strategy to improve the performance and immune system of animals. Aims The present study examined the effects of chromium-methionine (Cr-Met) and zinc-methionine (Zn-Met) supplementation on animal performance and response to transportation stress. Methods Eighteen lambs (18–20 weeks of age) were randomly assigned to the following three dietary treatments for 10 weeks: (1) basal diet (control, 0.021 g/kg Zn and 0.0001 g/kg Cr); (2) basal diet with addition of 10 Cr-Met mg/kg DM (containing 1 mg/kg Cr); and (3) basal diet with addition of 500 Zn-Met mg/kg DM (containing 50 mg/kg Zn). After a 6-week supplementation period, the lambs were transported by road for 3 h. Key results Mineral supplements did not affect final bodyweight, average daily gain or feed conversion ratio. The blood cortisol and aspartate aminotransferase concentrations were increased (P < 0.05) immediately after transportation (AT1) in all groups; however, they were lower in supplemented lambs than in the control (P < 0.05). Animals fed Cr-Met or Zn-Met diets had a higher albumin concentration at AT1 than did the control group (P < 0.05). Lambs on the Zn-Met diet showed a higher blood triiodothyronine and triiodothyronine to thyroxin ratio at AT1 and at 24 h after transportation (AT2) than did lambs receiving the control diet (P < 0.05). Lambs fed the Cr-Met diet had a lower blood malondialdehyde at AT1 and AT2 than did the control lambs (P < 0.05). Total antioxidant capacity (TAC) was higher in the Zn-Met-supplemented group than in other groups (P < 0.05). Supplementation with dietary Zn-Met increased serum TAC concentration in the kidney tissue compared with the control (P < 0.05), but had no effect on superoxide dismutase and glutathione peroxidase activities. The Cr-Met group showed also a lower malondialdehyde concentration (P < 0.05) and higher TAC, superoxide dismutase and glutathione peroxidase activities in the liver tissue (P < 0.05). Conclusions Dietary supplementation with 10 mg Cr-Met/kg (1 mg Cr) in comparison to 500 mg Zn-Met/kg (50 mg Zn) improved the stress response of lambs subjected to a short-time road transportation, whereas growth performance was not affected. Implications The administration of Cr-Met or Zn-Met to diet of lambs before transportation could reduce the adverse effects of road transportation stress.

Effects of supplementing layer hen diets with selenium and vitamin E on egg quality, lipid oxidation and fatty acid composition during storage

2008 ◽  
Vol 88 (3) ◽  
pp. 475-483 ◽  
Author(s):  
Maziar Mohiti-Asli ◽  
Farid Shariatmadari ◽  
Houshang Lotfollahian ◽  
Mohamad Taghi Mazuji
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Vitamin E ◽  
Acid Composition ◽  
Egg Quality ◽  
Basal Diet ◽  
Quality Parameters ◽  
Control Group ◽  
Selenium Yeast ◽  
Organic Selenium

A 7-wk trial was carried out to investigate the effect of vitamin E and inorganic and organic selenium added to hens' diet on quality and lipid stability of eggs during storage. One hundred forty-four Hy-Line W-36 hens (63-wk of age) were divided into six equal groups. Five groups received a basal diet supplemented with 0.4 mg kg-1 sodium selenite or selenium yeast, 200 mg kg-1 vitamin E or a combination of selenium and vitamin E; whereas the control group received no supplementation. Hen production was assessed daily and fresh egg quality parameters were determined every 2 wk. Eggs were stored under different conditions (4°C, 23–27°C or 31°C) for 14 d. Eggs were analyzed for quality characteristics, egg component weight, Malondialdehyde values as a secondary oxidation product and yolk fatty acid (FA) composition. The performance of the hens and egg weights were not affected either by the source of the selenium or by the vitamin E. The inclusion of selenium or vitamin E in the diet significantly increased their concentrations in the egg. The supplemented diets also improved egg quality, oxidative stability and fatty acid composition during storage. Key words: Vitamin E, selenium, lipid peroxidation, egg fatty acid composition, storage

scholarly journals Improving the Quality Characteristics and Shelf Life of Meat and Growth Performance in Goose Fed Diets Supplemented with Vitamin E

Foods ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 798
Author(s):  
Zabihollah Nemati ◽  
Kazem Alirezalu ◽  
Maghsoud Besharati ◽  
Saeid Amirdahri ◽  
Daniel Franco ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Vitamin E ◽  
Shelf Life ◽  
Growth Performance ◽  
Saturated Fatty Acids ◽  
Basal Diet ◽  
Fat Content ◽  
Dietary Vitamin ◽  
Control Group ◽  
Vitamin E Supplementation

The present study was carried out to investigate the effect of dietary vitamin E on growth performance, cellular immunity, carcass characteristics, and meat quality in geese. Sixty-four one-day-old male geese were selected from 1200 goose chicks with the same average body weight (92.5 ± 2.5 g) and subjected to two treatments (basal diet or control and basal diet plus 120 mg/kg vitamin E supplement) with 4 replicates (8 geese per replicate) for 8 weeks. After slaughter, goose meat was aerobically packed in polyethylene packages and stored at 4 °C for 9 days. The results showed that vitamin E supplementation improved the growth performance, carcass yield percentage, and immune response of goose (p < 0.05). The addition of vitamin E in the diet significantly increased the protein and fat content of goose meat but decreased the moisture and ash content with respect to those obtained from the control diet. During storage, meat from the vitamin E treatment showed higher phenolic content and lower thiobarbituric acid reactive substances (TBARSs) and total volatile nitrogen (TVB-N) values than those from the control treatment. Vitamin E supplementation increased the saturated fatty acids (SFAs), monounsaturated fatty acids (MUFAs), and polyunsaturated fatty acids (PUFAs) in goose meat. However, goose meat supplemented with vitamin E displayed a significantly (p < 0.05) higher PUFA/SFA ratio than those of the control group. Based on the results, it was concluded that vitamin E could be used to improve the growth performance of goose, the meat composition in terms of the protein and fat content, the nutritional value in terms of the fatty acid composition, and the shelf life.

scholarly journals Effects of Dietary Betaine on Growth Performance, Digestive Function, Carcass Traits, and Meat Quality in Indigenous Yellow-Feathered Broilers under Long-Term Heat Stress

Animals ◽  
2019 ◽  
Vol 9 (8) ◽  
pp. 506 ◽  
Author(s):  
Liu ◽  
Yuan ◽  
Sun ◽  
Balasubramanian ◽  
Zhao ◽  
...  
Keyword(s):  
Heat Stress ◽  
Growth Performance ◽  
Feed Intake ◽  
Nitrogen Retention ◽  
Basal Diet ◽  
Control Group ◽  
Trypsin Activity ◽  
Villus Height ◽  
Betaine Supplementation

Heat stress has a profound effect on poultry health and productivity. The present study evaluated whether feeding betaine could ameliorate long-term heat stress-induced impairment of productive performance in indigenous yellow-feathered broilers. A total of 240 five-week-old male broilers were randomly allocated to five treatments with six replicates of eight broilers each. The five treatments included a thermoneutral zone control group (TN, fed basal diet), a heat stress control group (HS, fed basal diet), and an HS control group supplemented 500, 1000, 2000 mg/kg betaine, respectively. The TN group was raised at 26 ± 1 °C during the whole study, HS groups exposed to 32 ± 1 °C for 8 h/day from 9:00 am to 17:00 pm. The results showed that heat stress decreased the body weight gain (BWG) and feed intake of broilers during 1–5, 6–10, and 1–10 weeks (p < 0.05). Dietary betaine tended to improve the BWG and feed intake of broilers under 5 weeks of heat stress (linear, p < 0.10), and betaine supplementation linearly increased the BWG and feed intake during 6–10 and 1–10 weeks (p < 0.05). Additionally, nitrogen retention was reduced by 5 weeks and 10 weeks of heat stress (p < 0.05), whereas dietary betaine could improve nitrogen retention in heat stressed broilers after both 5 and 10 weeks of heat stress (linear, p < 0.05). Moreover, this study observed that the trypsin activity of jejunum was decreased by 5 weeks of heat stress (p < 0.05), whereas betaine supplementation had quadratic effects on trypsin activity of jejunum in heat stressed broilers (p < 0.05). Furthermore, 10 weeks of heat stress induced a reduction of villus height of the duodenum and jejunum (p < 0.05), and decreased the villus height to crypt depth ratio of the jejunum (p < 0.05). Supplementation with betaine ameliorated the adverse effects of heat stress on these parameters (p < 0.05). Compared with the TN group, 10 weeks of heat stress reduced carcass and breast yield (p < 0.05) and betaine supplementation improved carcass and breast yield of heat stressed broilers (linear, p < 0.05). In conclusion, dietary supplementation of betaine could reduce the detrimental effects of long-term heat stress on growth performance, digestive function, and carcass traits in indigenous yellow-feathered broilers.

scholarly journals Bovine spermatozoa react to in vitro heat stress by activating the mitogen-activated protein kinase 14 signalling pathway

2014 ◽  
Vol 26 (2) ◽  
pp. 245 ◽  
Author(s):  
Mohammad Bozlur Rahman ◽  
Leen Vandaele ◽  
Tom Rijsselaere ◽  
Mohamed Shehab El-Deen ◽  
Dominiek Maes ◽  
...  
Keyword(s):  
Heat Stress ◽  
Protein Kinase ◽  
Embryo Development ◽  
Mapk Pathway ◽  
Specific Inhibitor ◽  
Signalling Pathway ◽  
Mitogen Activated Protein Kinase ◽  
Farm Animals ◽  
Control Group ◽  
Mitogen Activated Protein

Heat stress has long been recognised as a cause of subfertility in farm animals. The objectives of the present study were to elucidate the effect of heat stress on sperm function and involvement of the mitogen-activated protein kinase (MAPK) 14 signalling pathway. Spermatozoa incubated for 4 h at a physiological temperature (38.5°C) exhibited significantly (P < 0.05) reduced motility, plasma membrane integrity and mitochondrial potential compared with non-incubated spermatozoa; the reductions in these parameters were more severe following incubation at a hyperthermic (41°C) temperature (P < 0.01). Percentages of fertilisation and embryo development were highly affected in spermatozoa incubated at 41°C compared with non-incubated spermatozoa (P < 0.01). Similarly, embryo quality was adversely affected by sperm incubation at 41°C, as indicated by a higher apoptotic cell ratio in Day 7 blastocysts compared with that in the non-incubated control group (14.6% vs 6.7%, respectively; P < 0.01). Using SB203580 (10 µg mL–1), a specific inhibitor of the p38 MAPK pathway, during sperm hyperthermia reduced MAPK14 activation (24.9% vs 35.6%), increased sperm motility (45.8% vs 26.5%) and reduced DNA fragmentation (16.9% vs 23.4%) compared with the untreated control group, but did not improve subsequent fertilisation and embryo development. In conclusion, heat stress significantly affects the potential of spermatozoa to penetrate oocytes, as well as subsequent embryo development and quality. Notably, the data show that the MAPK14 signalling pathway is largely involved in heat-induced sperm damage. However, further research is needed to elucidate other signalling pathways possibly involved in heat-induced sperm damage.

scholarly journals Alleviating Effects of Vitamins C and E Supplementation on Oxidative Stress, Hematobiochemical and Histopathological Alterations Caused by Copper Toxicity in Broiler Chickens

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1739
Author(s):  
Mohamed A. Hashem ◽  
Sahar S. Abd El Hamied ◽  
Eman M. A. Ahmed ◽  
Shimaa A. Amer ◽  
Aziza M. Hassan
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
Vitamin C ◽  
Broiler Chickens ◽  
Copper Toxicity ◽  
Basal Diet ◽  
High Serum ◽  
Control Group ◽  
Broiler Chicks ◽  
High Doses

The current investigation evaluated the alleviating effects of vitamin C and vitamin E on oxidative stress, hematobiochemical, and histopathological changes in the kidney induced by copper sulfate (CuSO4) toxicity in chickens. Two hundred and fifty-one-day-old male broiler chicks were randomly allotted into five experimental groups (five replicates/group, ten chicks/replicate): 1st group—basal diet with no additives (control group), 2nd group—basal diet complemented with CuSO4 (300 mg/kg diet), 3rd group—basal diet with CuSO4 (300 mg/kg diet) + vitamin C (250 mg/kg diet), 4th group—basal diet with CuSO4 (300 mg/kg diet) + vitamin E (250 mg/kg diet), and 5th group—basal diet with CuSO4 (300 mg/kg diet) + vitamin C (250 mg/kg diet) + vitamin E (250 mg/kg diet) for a 42 day feeding period. The results showed a significant reduction in red blood cells (RBCs), hemoglobin (Hb) concentration, and hematocrit values as well as total leukocyte counts (WBCs), lymphocyte, heterophil, and monocyte counts in the CuSO4-intoxicated birds (2.42 × 106/µL, 9.54 g/dL, 26.02%, 15.80 × 103/µL, 7.86 × 103/µL, 5.26 × 103/µL, and 1.18 × 103/µL, respectively, at the 6th week) compared to (2.79 × 106/µL, 10.98 g/dL, 28.46%, 21.07 × 103/µL, 10.84 × 103/µL, 7.12 × 103/µL, and 1.60 × 103/µL, respectively) in the control group. Moreover, CuSO4-intoxicated birds showed hypoglycemia with a rise in serum uric acid and creatinine levels (122.68, 5.18, and 0.78 mg/dL at the 6th week) compared to (159.46, 4.41, and 0.61 mg/dL) in the control group. The CuSO4 toxicity in birds induced oxidative stress, indicated by a high serum malondialdehyde level (MDA) and diminished activity of the antioxidant enzymes (glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD)) (2.01 nmol/mL, 37.66 U/mL, and 2.91 U/mL, respectively, at the 6th week) compared to (1.34 nmol/mL, 57.00 U/mL, 4.99 U/mL, respectively) in the control group. High doses of Cu exposure caused severe microscopic alterations in kidney architecture. The addition of vitamins C and E, singularly or in combination, displayed a beneficial effect in alleviating these harmful effects of Cu toxicity. These findings showed the possible mitigating impacts of dietary antioxidants on the hematobiochemical alterations, oxidative stress, and kidney damage induced by CuSO4 toxicity.

scholarly journals Protective effect of vitamin E on sperm parameters, chromatin quality, and DNA fragmentation in mice treated with different doses of ethanol: An experimental study

2021 ◽  
Author(s):  
Mohamad Reza Doostabadi ◽  
Mohammadmehdi Hassanzadeh-taheri ◽  
Mahmoud Asgharzadeh ◽  
Masoomeh Mohammadzadeh
Keyword(s):  
Vitamin E ◽  
Dna Fragmentation ◽  
Sperm Quality ◽  
Dna Structure ◽  
Basal Diet ◽  
Sperm Parameters ◽  
Sperm Chromatin ◽  
Control Group ◽  
Protective Effects ◽  
Normal Morphology

Background: Excessive consumption of alcohol induces an increase in oxidative stress production and can lead to detrimental effects on the male reproductive system. Objective: To evaluate the possible protective effects of coadministration of vitamin (vit) E on the detrimental changes in the sperm quality of mice administered ethanol. Materials and Methods: Fifty-four BALB/c mice were categorized into nine groups (n = 6/each). The control group received a basal diet while the eight experimental groups received ethanol 10%; ethanol 20%; vit. E 100 mg; vit. E 200 mg; ethanol 10% + vit. E 100 mg; ethanol 10% + vit. E 200 mg; ethanol 20% + vit. E 100 mg; ethanol 20% + vit. E 200 mg. After 35 days, the sperm parameters and sperm chromatin were assessed. Results: The results demonstrated a significant reduction in the motility rate, normal morphology rate, viability rate, increase in abnormal DNA structure and packaging (TB staining), and DNA damage (TUNEL) in ethanol consumer groups. In addition, the findings showed a significant increase in the aforementioned parameters in ethanoland vit. E-consumer groups compared to the ethanol-only consumer groups. The ethanol group received 20% of the most damage among the groups. The group receiving vit. E 100 mg and those receiving ethanol 10% + vit. E 200 mg gained the highest benefit among the groups. Conclusion: Sperm forward progressive motility, normal morphology rate, and viability decreased in the ethanol groups. Also, the rates of spermatozoa with abnormal DNA structure and DNA fragmentation increased in the ethanol groups. Our findings revealed that the coadministration of vit. E and ethanol can protect destructive changes in DNA structure and damage. Key words: Ethanol, Sperm parameters, Vitamin E.

scholarly journals Effects of organic zinc on the performance and gut integrity of broilers under heat stress conditions

2020 ◽  
Vol 63 (1) ◽  
pp. 125-135
Author(s):  
Mohannad Abuajamieh ◽  
Anas Abdelqader ◽  
Rabie Irshaid ◽  
Firas M. F. Hayajneh ◽  
Ja'far M. Al-Khaza'leh ◽  
...  
Keyword(s):  
Heat Stress ◽  
Average Daily Gain ◽  
Morphological Characteristics ◽  
Basal Diet ◽  
Stress Conditions ◽  
Farm Animals ◽  
Final Body Weight ◽  
Blood Calcium ◽  
Organic Zinc ◽  
Organic Source

Abstract. Heat stress (HS) has negative impacts on farm animals. Many studies have been conducted in order to ameliorate the effects of heat stress in farm animals. The current project investigated the effects of organic zinc supplementation under thermoneutral and heat stress conditions on the production, physiological, and histological parameters in broiler chickens. Three-hundred and sixty chicks in the current project were assigned randomly to six different treatments (n=60 chicks per treatment). The treatments were (1) a basal diet containing 40 mg kg−1 of Zn from an organic source and rearing under thermoneutral (TN) conditions (Ctrl); (2) a diet containing the amount of Zn from the basal diet +50 % of the Zn level (from the basal diet) and rearing under TN conditions (50 TN); (3) a diet containing the amount of Zn from the basal diet +100 % of the Zn level (from the basal diet) and rearing under TN conditions (100 TN); (4) a basal diet containing 40 mg kg−1 of Zn from an organic source and exposure to 3 d of cyclical HS at the age of 35 d (CHS); (5) a diet containing the amount of Zn from the basal diet +50 % of the Zn level (from the basal diet) and exposure to 3 d of cyclical HS at the age of 35 d (50 HS); and (6) a diet containing the amount of Zn from the basal diet +100 % of the Zn level (from the basal diet) and exposure to 3 d of cyclical HS at the age of 35 d (100 HS). Our results indicated that HS has decreased final body weight (fBW), average daily gain (ADG), and feed conversion ratio (FCR) relative to TN chicks. However, organic zinc had little or no effects on the production parameters measures in the current project. Overall, intestinal histological measurements were negatively altered under HS relative to TN chicks. Organic zinc inclusion in the diet had improved villus height in the duodenum and jejunum relative to the Ctrl and CHS chicks. Blood calcium and glucose levels were decreased and increased, respectively, in HS relative to TN chicks. In summary, the results discussed in the current project revealed that the inclusion rates of organic zinc used here had little or no effects on the productive parameters. However, it improved the morphological characteristics of the intestines which might maximized the intestinal efficiency in nutrient absorption under HS conditions.

scholarly journals Comparative analysis of various sources of selenium on the growth performance and antioxidant status in broilers under heat stress

2023 ◽  
Vol 83 ◽  
Author(s):  
F. Gul ◽  
B. Ahmad ◽  
S. Afzal ◽  
A. Ullah ◽  
S. Khan ◽  
...  
Keyword(s):  
Heat Stress ◽  
Comparative Analysis ◽  
Basal Diet ◽  
Oxidative Capacity ◽  
Negative Control ◽  
Selenium Supplementation ◽  
Control Group ◽  
Selenium Yeast ◽  
Response Level ◽  
Organic Selenium

Abstract The supplementation of Selenium-enriched probiotics is effective in reducing oxidative stress and maintaining meat quality stability in broiler chicken especially under heat stress. An experimental study was conducted to perform Comparative analysis of Selenium yeast with inorganic Se in broilers under heat stress. A total of 120 broilers chicks of one day were assigned to 4 groups each consisting 30 chicks fed on same basal diet but different selenium sources. The basal diet of group D1 was not supplemented with Se source (Negative control), group D2 basal diet was supplemented with inorganic selenium (Sodium selenite 0.22mg/Kg starter phase and 0.15mg/Kg finisher phase), group D3 basal diet was supplemented with commercially available organic selenium (Seleno-methionine 0.22mg/Kg starter phase and 0.15mg/Kg finisher phase) and group D4 basal diet was supplemented with self-developed organic selenium (Se-enriched yeast 0.22mg/Kg starter phase and 0.15mg/Kg finisher phase). The performance parameters i.e. feed intake (FI), live body weight (BW) and FCR were not significantly (p>0.05) effected by selenium supplementation in the starter phase but were significantly (p<0.05) effected in the finisher phase. Selenium supplementation significantly (p<0.05) effected serum Se level in different supplemented groups. Higher serum Se value (58.20±0.06) was recorded in D4 group. Similarly significantly lower selenium value was recorded for D4 and higher was recorded for D1 (11.36±0.08). However lower serum Paraoxonase (PON) value was recorded for D4 (13.24±0.01) and higher for D1 (13.33±0.03). Comparatively self-developed Se enriched yeast increased the Se accumulation and improved antioxidant system. Glutathione peroxidase (GPx) was found higher in D4 (12.333±0.03) followed by D3, D2 and D1 respectively. Whereas superoxide dismutase (SOD) was significantly lower (p<0.05) in D4 (0.1437±0.003) followed by D3 (0.1457±0.002). Selenium supplementation increased the bird’s survival rate. Birds fed on Se enriched yeast showed higher Se deposition and better antioxidant capacity as compared to other sources of selenium. Se-enriched yeast displayed an improved result on Se deposition in tissues, and oxidative capacity, meat tenderness and immune response level as compared to other sources of selenium.

scholarly journals Hydroxy Selenomethionine Improves Meat Quality through Optimal Skeletal Metabolism and Functions of Selenoproteins of Pigs under Chronic Heat Stress

Antioxidants ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 1558
Author(s):  
Yan Liu ◽  
Shenggang Yin ◽  
Jiayong Tang ◽  
Yonggang Liu ◽  
Gang Jia ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Heat Stress ◽  
Growth Performance ◽  
Meat Quality ◽  
Carcass Traits ◽  
Basal Diet ◽  
Growing Pigs ◽  
Control Group ◽  
Encoding Genes

Chronic heat stress (CHS) induces metabolic changes in skeletal muscle from growth to maintenance that jeopardizes growth performance, carcass traits, and meat quality of pigs. We investigated the protective effect of dietary organic selenium (hydroxy-4-methylselenobutanoic acid, OH-SeMet) on CHS-induced skeletal muscle damages of growing pigs, and the corresponding responses of selenoproteins. A total of 40 ((Landrace × Yorkshire) × Duroc) pigs with an average live weight of 49.64 ± 2.48 kg were used in this 4-week trial. Pigs were randomly allotted to 5 groups: The control group was raised on a basal diet in a thermoneutral environment (22 ± 2 °C); and four CHS groups were raised on a basal diet and supplemented with Se 0.0, 0.2, 0.4, and 0.6 mg/kg as OH-SeMet, respectively, in hyperthermal condition (33 ± 2 °C). CHS resulted in significant decrease of growth performance, carcass traits, and meat quality, which were associated with reduced (p < 0.05) serum alkaline phosphatase (ALP) and total superoxide dismutase (T-SOD) and increased (p < 0.05) serum creatine (CK), sarcous heat shock protein 70 (HSP70), glucokinase (GCK), phosphoenolpyruvate carboxykinase (PEPCK), and malondialdehyde (MDA) contents. Meanwhile, four metabolism-related genes and seven selenoprotein encoding genes were abnormally expressed in skeletal muscle. Dietary OH-SeMet addition partially alleviated the negative impact of CHS on carcass traits and improved meat quality. These improvements were accompanied by the increase in Se deposition, the anti-oxidative capacity of serum and muscle, and protein abundance of GPX1, GPX3, GPX4, and SELENOP. Supplementation with 0.6 mg Se/kg (OH-SeMet) restored the sarcous PEPCK, and 0.4 and 0.6 mg Se/kg (OH-SeMet) restored all abnormally expressed metabolism-related and selenoprotein encoding genes. In summary, dietary supplementation with OH-SeMet beyond Se requirement mitigated CHS-induced depression of carcass traits and meat quality of pigs associated with optimal skeletal metabolism, enhanced antioxidant capacity, and regulation of selenoproteins in skeletal muscle of pigs.

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