Increased Brain Derived Neurothropic Factor in the cerebrum and cerebellum of Rattus norvegicus newborn with exposure to Mozart's music in default sequence compared with the reversed sequence and without exposure during gestation

Objectives: To analyze the differences in the expression of Brain Derived Neurothropic Factor (BDNF) in Rattus norvegicus cerebrum and cerebellum of newborn between those exposed to Mozart's music composition in default sequence, reverse sequence, and without exposure in the womb.Materials and Methods: Analytical laboratory experimental study with randomized post test only control group design using animal models Rattus norvegicus. The animal models were divided into three groups: control group without any exposure, the treatment groups with exposure to Mozart's music in default sequence and another group in reverse sequence since day 10 of gestation. We used a comparison test in the analysis of BDNF expression.Results: We found significant difference in BDNF expression with p value 0.004 (mean 8.98±1.31 default sequence group, 5.58±3.08 reverse sequence group, 6.80±1.95 control) in the cerebrum. We found significant difference of BDNF expression with p value 0.003 (mean 9.48±1.41 default sequence group, 6.02±3.25 reverse sequence group, 7.14±2.54 control) in the cerebellum. In cerebrum dan cerebellum we found significant difference between standard Mozart’s music and control (cere-brum p=0.018, cerebellum p=0.001), and we found significant difference between standard Mozart’s music and reverse Mozart’s music (cerebrum p=0.001, cerebellum p=0.008) and no significant difference in reverse Mozart and control (cerebrum p=0.264, cerebellum p=0.490)Conclusion: Sequence in Mozart’s music is very important in increase expression of BDNF.

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EFFECT OF FAMILY PSYCHOEDUCATION ON CAREGIVER SUPPORT IN THE TREATMENT OF PATIENTS WITH TYPE II DIABETES MELLITUS

Belitung Nursing Journal ◽

10.33546/bnj.342 ◽

2018 ◽

Vol 4 (1) ◽

pp. 112-119

Author(s):

Andika Siswoaribowo ◽

Mateus Sakundarno ◽

Muhammad Mu’in

Keyword(s):

Diabetes Mellitus ◽

T Test ◽

Control Group ◽

P Value ◽

Family Psychoeducation ◽

Control Group Design ◽

Caregiver Support ◽

Significant Difference ◽

Patients With Diabetes ◽

And Control

Background: Diabetes Mellitus is a chronic disease that requires treatment for long periods of time so it can cause physical and psychological problems for sufferers and families (caregiver). Caregiver's role is expected to provide support for people with diabetes mellitus. Family psychoeducation is a strategy that can be applied for caregiver in overcoming problems that arise during the treatment of patients with diabetes mellitus.Objective: This study aims to determine the effect of family psychoeducation on caregiver support in the treatment of patients with diabetes mellitus type II.Methods: This research used a quasi experiment with pre-test post-test control group design. A total of 46 caregivers and patients with diabetes mellitus were recruited puposively, with 23 respondents assigned in the experiment and control group. Caregiver support scale was used to measure caregiver support, and Hensarling Diabetes Family Support Scale (HDFSS) for measuring perception of patients toward the treatment of caregiver. Data were analyzed using paired t-test and independent t-test. Results: Findings showed there was an increase of caregiver knowledge from 5.39 to 9.09 and an improvement of caregiver treatment from 40.30 to 67.04 after given family psychoeducation. There was a significant difference of caregiver support in the experimental and control group with p-value <0.001 (<0.05).Conclusion: Family psychoeducation can increase caregiver support in the treatment of diabetes mellitus patients. The intervention can be one of nurses interventions in the empowerment of family in improving the treatment of chronic diseases, particularly in diabetes mellitus.

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Effects of Curcumin on Vascular Endothelial Growth Factor Expression on Rattus norvegicus Cervical Cancer Xenograft Model

Indonesian Journal of Cancer ◽

10.33371/ijoc.v12i3.616 ◽

2019 ◽

Vol 12 (3) ◽

pp. 95

Author(s):

Laili Muninggar ◽

Widjiati Widjiati ◽

Indra Yuliati ◽

Brahmana Askandar ◽

Poedjo Hartono

Keyword(s):

Cervical Cancer ◽

Rattus Norvegicus ◽

Histopathological Examination ◽

Xenograft Model ◽

Control Group ◽

P Value ◽

Vegf Expression ◽

Control Group Design ◽

Significant Difference ◽

Median Expression

Objective: To analyze the effect of curcumin in VEGF expression on Rattus norvegicus cervical cancer cell xenograft model.Methods: An experimental study with randomized post test only control group design. The subjects were Rattus norvegicus (Sprague Dawley), inoculated with He-la cervical cancer cells from American Type Culture Collection (ATCC) processed in stem cell laboratory Institute of Tropical Disease (ITD) Airlangga University. 5x106 of He-La cells were injected subcutaneously in dorsal flank area of Rattus norvegicus. After 30 days of observation we performed histopathological examination of xenograft tissue and randomized into 2 groups which were given curcumin orally 1000 mg/kg (curcumin group) vs. no therapy (control group). After another 30 days the xenograft tissue was dissected and underwent immunochemistry examination for VEGF expression.Results: 32 samples of Rattus norvegicus were divided into 2 groups, In curcumin group the VEGF median expression was 2,2 (0,3-7,6) and in control group the VEGF median expression was 6,6 (1,2-12). There was a statistically significant difference with p value =0,009 with Mann Whitney test (p<0,05).Conclusion: VEGF expression in Rattus norvegicus xenograft model of cervical cancer was suppressed by giving Curcumin 1000 mg/kgBB orally.

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Effect of Giving Mahkota Dewa Fruits (Phaleria Macrocarpa) Extract to Epithelialization In Incision Wound of White Rats (Rattus Norvegicus)

Jurnal Ilmiah Keperawatan STIKES Hang Tuah Surabaya ◽

10.30643/jiksht.v12i1.37 ◽

2019 ◽

Vol 12 (1) ◽

Author(s):

Idola Perdana Sulistyoning Suharto

Keyword(s):

Treatment Group ◽

Rattus Norvegicus ◽

Male Rats ◽

Control Group ◽

P Value ◽

Control Group Design ◽

White Rats ◽

Significant Difference ◽

Phaleria Macrocarpa ◽

Incision Wound

The research purpose was to analysis effect of giving mahkota dewa fruits (Phaleria macrocarpa) extract to epithelialization in incision wound of white rats (Rattus norvegicus). The method was randomized posted-only control group design. There were 30 male rats (Rattus norvegicus) grouped on control and treatment group. Control group divided into three groups (KK1, KK2, KK3) and also treatment group divided into three groups (KP1, KP2, KP3). Control group just given CMC 1% peroral without mahkota dewa fruits extract, the treatment group given mahkota dewa fruits extract 22.5 mg/kg body weight. The data was analyzed by Kruskall Wallis. Based on Kruskall Wallis test, obtained result that there was a significant difference (p<0.05) epithelialization variable with p value p = 0.000 between control and treatment group. And based on One-way Anova test, obtained result that there was a significant difference (p<0.05) with p value p =0.000 between control and treatment group. The conclusion of this research was giving mahkota dewa fruits (Phaleria macrocarpa) extract can increase epithelialization in incision wound of white rats (Rattus norvegicus). Keywords : Mahkota Dewa Fruits (Phaleria Macrocarpa) Extract, Epithelialization, Incision Wound

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Pengaruh Latihan Renang Teratur dan Latihan Renang Tidak Teratur Terhadap Kadar Trigliserida Plasma pada Rattus norvegicus

BIOTROPIC The Journal of Tropical Biology ◽

10.29080/biotropic.2018.2.1.24-40 ◽

2018 ◽

Vol 2 (1) ◽

pp. 24-40

Author(s):

Adi Wijayanto

Keyword(s):

Rattus Norvegicus ◽

Exercise Program ◽

The Body ◽

Control Group ◽

Regular Exercise ◽

Control Group Design ◽

Group Design ◽

Significant Difference ◽

Post Test ◽

And Control

Triglycerides are source of energy in the body, but triglyceride deposits that exceed the threshold will cause health problems, so triglycerides should be lowered to normal. The reduction of triglycerides can be done through physical activity, therefore this study aims to reveal the effect of regular swimming exercises and irregular exercises against triglyceride levels. This research is an experimental research using The Post Test Only Control Group Design design. Subjects of the study were male of Rattus Norvegicus Wistar, 39 subjects was divided into 3 groups with Matched Subject Design technique, which consisted of 2 groups of treatment that is regular and irregular group, and control group. The exercise program was given a swimming with a 3% weight of body weight and was tied 5 cm from the tip of the tail which was performed 3 times per week consistently for regular exercise and 0 to 5 times per week inconsistently for irregular exercise for 12 weeks, While the control group was not given training. In the last week before the blood was taken, the first three groups were given swimming treatment exercises. Based on the results of the anava test, there was a significant difference in the reduction of triglyceride levels among white mice who were given regular exercise with irregular exercise. These results suggest that regular exercise has a better impact on triglyceride reduction than irregular exercise, due to the higher adaptation process of regular exercise.

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EFFECT OF ROSELLE (HIBISCUS SABDARIFFA) ON CHANGES IN HEMOGLOBIN LEVELS IN PREGNANT WOMEN WITH ANEMIA TAKING IRON SUPPLEMENT

Belitung Nursing Journal ◽

10.33546/bnj.305 ◽

2017 ◽

Vol 3 (6) ◽

pp. 771-777

Author(s):

Rif’atun Nisa ◽

Ariawan Soejoenoes ◽

Sri Wahyuni

Keyword(s):

Pregnant Women ◽

Hemoglobin Level ◽

T Test ◽

Control Group ◽

P Value ◽

Hibiscus Sabdariffa ◽

Control Group Design ◽

Significant Difference ◽

And Control ◽

Paired T Test

Background: Anemia during pregnancy is one of the most common disorders in pregnant women in Indonesia. The Government has made efforts to overcome this problem, however, the rate of anemic mothers remains high. Rosella (Hibiscus Sabdariffa) is considered able to increase the hemoglobin levels in pregnant mothers.Objective: To analyze the effect of Rosella flower extract (Hibiscus Sabdariffa) on the increase of Hemoglobin level in pregnant women with anemia receiving Fe tablet.Methods: This study was a quasy experiment with pretest-posttest control group design conducted in November - December 2016 in the working area of Tlogosari Wetan Community Health Center. Forty-two participants were selected using accidental sampling, which 21 assigned in the experiment and control group. All samples were pregnant women in the second trimester suffering from anemia and receiving iron tablets. Hemoglobin levels were measured using hematology analyzer in laboratory. Independent t-test and paired t-test were used for data analysis.Results: Paired t-test obtained p-value 0.00 (<0.05), indicated that there was an increase of hemoglobin levels in both experiment and control group. The mean increase of hemoglobin levels in the control group was 0.61 gr and in the experiment group was 1.08. The hemoglobin levels in the experiment group were higher than the levels in the control group. Independent t-test obtained p-value 0.000 (<0.05) indicating that there was a significant difference of mean of hemoglobin levels between the control group and the treatment group.Conclusion: The consumption of rosella extract combined with Fe tablet showed a significant increase of hemoglobin levels compared with the consumption of Fe tablet alone. Therefore, it is suggested for midwife to use the result of this research as a evidence practice through counseling for pregnant mother about utilization of rosella extract that can increase hemoglobin level in pregnant woman with anemia.

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Penyuluhan dan Pengetahuan Tentang Pernikahan Usia Muda

Kesmas National Public Health Journal ◽

10.21109/kesmas.v8i2.343 ◽

2013 ◽

Vol 8 (2) ◽

pp. 56

Author(s):

Rufaida Nurjanah ◽

Dwiana Estiwidani ◽

Yuliasti Eka Purnamaningrum

Keyword(s):

Young Age ◽

Average Score ◽

Control Group ◽

P Value ◽

Control Group Design ◽

Group Design ◽

Significant Difference ◽

Quasi Experimental ◽

The Subject ◽

And Control

Pada tahun 2010-2012, di Kabupaten Gunungkidul, terjadi kenaikan dua kali lipat kasus pernikahan di bawah umur. Kasus tertinggi terdapat di Kecamatan Patuk, yaitu sebanyak 18 kasus. Kehamilan di usia muda berkorelasi dengan angka kematian ibu. Peningkatan pengetahuan kesehatan reproduksi dapat dilakukan dengan penyuluhan. Tujuan penelitian ini untuk mengetahui pengaruh penyuluhan terhadap peningkatan pengetahuan tentang pernikahan usia muda. Jenis penelitian yang digunakan adalah kuasi eksperimen dengan pretest-posttest terhadap kelompok kontrol. Penelitian dilakukan di SMPN 1 Patuk. Subjek penelitian adalah siswa kelas VIII. Jumlah sampel sebanyak 25 responden, baik pada kelompok eksperimen maupun kontrol. Instrumen penelitian adalah kuesioner. Hasil pretest menunjukkan nilai rata-rata kelompok eksperimen adalah 70,40 dan kelompok kontrol adalah 71,20. Hasil posttest menunjukkan nilai rata-rata kelompok eksperimen adalah 90,88 dan kelompok kontrol adalah 78,40. Terdapat perbedaan yang signifikan antara nilai pretest dengan posttest. Peningkatan pengetahuan pada kelompok eksperimen sebesar 20,48, sedangkan kelompok kontrol sebesar 7,20. Hasil uji independen sampel uji t menghasilkan nilai p 0,000 (< 0,05). Penelitian ini menyimpulkan bahwa ada pengaruh penyuluhan terhadap peningkatan pengetahuan tentang pernikahan usia muda pada siswa kelas VIII di SMPN 1 Patuk tahun 2013.In 2010-2012, the amount of underage marriage had doubled in Gunung Kidul. Moreover, the highest case was in Patuk which had 18 cases. However, there was correlation between early pregnancy and maternal mortality. Improving the knowledge of reproductive health can be done by counseling. The research was aimed to find out about the effect of counseling on the improvement of the knowledge of young age marriage. The research was categorized into quasi experimental research which has pre-posttest with control group design. The research was conducted at SMPN 1 Patuk. The subject of the research was VIII grade students. There were 25 respondents both the experiment group and control group as well. The instrument used was questioner. The result of pretest was the average score of experiment group was 70.40 while control group’s average score is 71.20. The result of the posttest was the average score of experiment group was 90.88 while the control group’s average score was 78.40. So, it could be concluded that there were a significant difference between pretest and posttest. The knowledge of experiment group increased by 20.48 whiles the control group’s knowledge increased by 7.20. The result of independent sample t-test was the score of p-value is 0.000 (< 0.05). This research concludes that there is effect of counseling on the improvement of the knowledge about young age marriage of VIII grade students of SMPN 1 Patuk in 2013.

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EFEKTIVITAS INTERVENSI PSIKOEDUKASI TERHADAP KEPATUHAN BEROBAT PASIEN SKIZOFRENIA

Real in Nursing Journal ◽

10.32883/rnj.v1i1.226 ◽

2018 ◽

Vol 1 (1) ◽

pp. 1

Author(s):

Neila Sulung ◽

Nice Foresa

Keyword(s):

Health Education ◽

Treatment Compliance ◽

Control Group ◽

P Value ◽

Control Group Design ◽

Psychosocial Therapy ◽

Significant Difference ◽

The Mean ◽

And Control ◽

Experimental Group

Psychoeducation is one form of intervention, both for families and clients who are part of the psychosocial therapy. Psychoeducation aims to increase knowledge about schizophrenia patients and families and to improve treatment compliance. This study aims to determine the effectiveness of psychoeducation intervention for treatment compliance of schizophrenia patient. The kind of this research is quasiexperimentalwith control group design with 16 samples that was selected by pusposive sampling, consist of 8 people was the experimental group that was given psychoeducation intervention and 8 people was the control group that was given health education. The datawas collected by filled the observation sheet as much as 12 statements. The Test that used was T-Test Independent with p value <0.05. The result of this research is the mean of treatment compliance on experimental group is 11,00 and control group 6,88. In statistic test showed that there is the significant difference between treatment compliance of schizopheria patient on experimental group and control group with p value 0,0005 (p < 0,05).Based of the result above can be concluded than psychoeducation intervention more effective to increase the treatment compliance of schizophrenia patients than health education. It is recomended to the nurse in health center to implements this psychoeducation regularly in gives nursing care to schizophrenia patients. Keywords : psychoeducation, treatment compliance, schizophrenia

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Default sequence of Mozart’s compositions during pregnancy gave higher dendritic density in the cerebrum and cerebellum of Rattus norvegicus offsprings compared with reversed sequence and control

Majalah Obstetri & Ginekologi ◽

10.20473/mog.v1i12019.5-11 ◽

2019 ◽

Vol 27 (1) ◽

pp. 5

Author(s):

Harry Mangasi Binsar Panjaitan ◽

Hermanto Tri Joewono ◽

Widjiati Widjiati

Keyword(s):

Experimental Study ◽

Treatment Group ◽

Rattus Norvegicus ◽

Control Group ◽

Exposure Group ◽

Control Group Design ◽

Group Design ◽

Significant Difference ◽

Post Test ◽

And Control

Objectives: This study aim to determine sequence of Mozart songs in the analyzing differences in dendritic density of cerebellum and cerebellum of new born Rattus novergicusMaterials and Methods: Experimental study randomized post test only control group design using Rattus norvegicus. Animal subjects were divided into three groups which were control group, and the treatment group that were given exposure to default sequence and reversed sequence of Mozart's music from gestation day 10. We used a comparison test in the analysis expression of BDNF.Results: In the cerebrum there was a significant difference in the Mozart group in reverse rather than with Mozart standard sequence and in standard Mozart group with no exposure group, with p=0.003 and p=0.000. In the cerebellum there was a significant difference in the group in reverse rather than with standard Mozart and in the standard Mozart group with the control group, with p=0.000 and p=0.000. However, there was no significant difference between control group and Mozart group upside down in cerebrum and cerebellum with p=0.109 and p=0.077Conclusion: The density of dendrites in the cerebrum and cerebellum of Rattus norvegicus newborn exposed to Mozart's music during pregnancy with standard Mozart sequence was higher than that in those receiving reverse order and without exposure. There were no significant differences between the density of the cerebrum and cerebellum dendrites between groups exposed to reversed sequence of Mozart composition and those without exposure.

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THE IMPACT OF COMBINATION OF ROLLING AND OKETANI MASSAGE ON PROLACTIN LEVEL AND BREAST MILK PRODUCTION IN POST-CESAREAN SECTION MOTHERS

Belitung Nursing Journal ◽

10.33546/bnj.150 ◽

2017 ◽

Vol 3 (4) ◽

pp. 329-336

Author(s):

Nia Dwi Yuliati ◽

Hadi Hadi ◽

Sri Rahayu ◽

Noor Pramono ◽

Donny Kristanto Mulyantoro

Keyword(s):

Breast Milk ◽

Cesarean Section ◽

Milk Production ◽

Control Group ◽

P Value ◽

Control Group Design ◽

Breast Milk Production ◽

Significant Difference ◽

And Control ◽

The Impact

Background: Normal childbirth or cesarean section has an effect on the initiation of early breastfeeding. Thus, rolling massage and oketani massage are considered helpful for milk production. However, little is known about the combination of rolling and oketani massage.Objective: To examine the effect of combination of rolling and oketani massage on the levels of prolactin and milk production in post-cesarian section mothers.Methods: A quasy-experimental study with pretest-posttest control group design. Thirty six samples were selected using consecutive sampling, with 18 each assigned to an experiment and control group. Breast milk production was measured based on the baby's weight, while prolactin levels were measured using ELISA method. Data were analyzed using univariate and bivariate analysis.Results: Results showed that there was a statistically significant difference of prolactin levels after intervention in the experiment and control group with p-value 0.035 (<0.005), and significant difference in breast milk production in both groups in posttest 1 and posttest 2 with p-value 0.000 (<0.05).Conclusion: There was statistically significant effect of the combination of rolling and oketani massage on the increase of prolactin levels and breast milk production. It is suggested that this intervention can be applied in midwifery care in post-cesarean section mothers.

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FR 50% in pregnancy results in different neuron and glial cell count (astrocytes, olygodendrocytes, and microglia) in the cerebrum and cerebellum of newborn Rattus norvegicus

Majalah Obstetri & Ginekologi ◽

10.20473/mog.v27i22019.56-65 ◽

2019 ◽

Vol 27 (2) ◽

pp. 56

Author(s):

Fitria Desky ◽

Hermanto Tri Joewono ◽

Widjiati Widjiati

Keyword(s):

Cell Count ◽

Glial Cell ◽

Rattus Norvegicus ◽

Food Restriction ◽

Control Group ◽

Control Group Design ◽

Microglia Cell ◽

Significant Difference ◽

And Control ◽

Veterinary Faculty

Objectives: To analyze the difference neuronal and glial (astrocytes, oligodendrocyte, microglia) cell count in cerebrum and cerebellum of Rattus norvegicus newborns with 50% food restriction and control group.Materials and Methods: This was an analytical experimental study with single blind randomized post test only control group design using animals subjects Rattus norvegicus. This study was conducted at Animal laboratory, Veterinary Faculty, Universitas Airlangga. Animal subjects were divided into FR50% group and control. Neuron and glial (astrocytes, oligodendrocytes, microglia) counts were analyzed using comparison test, with CI 95%.Results: There was a significant difference in cerebrum and cerebellum neuron cell count between intervention and control group (9.88+3.59 vs 16.88+2.553; p=0.000 and 7.5+1.789 vs 11.44+4.56; p=0.02). There was no difference in cerebrum and cerebellum glial cell count. There was a significant difference in cerebellum astrocyte between intervention and control group (80.94+24.255 vs 59.69+18.77; p=0.02) but no difference in cerebrum. There was a significant difference in cerebrum and cerebellum oligodendrocyte between intervention and control group (14.06+12.195 vs 25.13+8.609; p<0.000 and 13.63+6.712 vs 24.00+8.862; p=0.001), and there were significant difference in cerebrum and cerebellum microglia cell between intervention and control group (5.25+3.435 vs 4.94+3.838; p=0.620 and 8.81+4.119 vs 5.25+1.483; p=0.004).Conclusion: Food Restriction 50% (FR50%) in Rattus norvegicus decreased cerebrum and cerebellum neuron cell and oligodendrocyte count and increased cerebrum and cerebellum microglial count.

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