Effects of Curcumin on Vascular Endothelial Growth Factor Expression on Rattus norvegicus Cervical Cancer Xenograft Model

Objective: To analyze the effect of curcumin in VEGF expression on Rattus norvegicus cervical cancer cell xenograft model.Methods: An experimental study with randomized post test only control group design. The subjects were Rattus norvegicus (Sprague Dawley), inoculated with He-la cervical cancer cells from American Type Culture Collection (ATCC) processed in stem cell laboratory Institute of Tropical Disease (ITD) Airlangga University. 5x106 of He-La cells were injected subcutaneously in dorsal flank area of Rattus norvegicus. After 30 days of observation we performed histopathological examination of xenograft tissue and randomized into 2 groups which were given curcumin orally 1000 mg/kg (curcumin group) vs. no therapy (control group). After another 30 days the xenograft tissue was dissected and underwent immunochemistry examination for VEGF expression.Results: 32 samples of Rattus norvegicus were divided into 2 groups, In curcumin group the VEGF median expression was 2,2 (0,3-7,6) and in control group the VEGF median expression was 6,6 (1,2-12). There was a statistically significant difference with p value =0,009 with Mann Whitney test (p<0,05).Conclusion: VEGF expression in Rattus norvegicus xenograft model of cervical cancer was suppressed by giving Curcumin 1000 mg/kgBB orally.

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Effect of Giving Mahkota Dewa Fruits (Phaleria Macrocarpa) Extract to Epithelialization In Incision Wound of White Rats (Rattus Norvegicus)

Jurnal Ilmiah Keperawatan STIKES Hang Tuah Surabaya ◽

10.30643/jiksht.v12i1.37 ◽

2019 ◽

Vol 12 (1) ◽

Author(s):

Idola Perdana Sulistyoning Suharto

Keyword(s):

Treatment Group ◽

Rattus Norvegicus ◽

Male Rats ◽

Control Group ◽

P Value ◽

Control Group Design ◽

White Rats ◽

Significant Difference ◽

Phaleria Macrocarpa ◽

Incision Wound

The research purpose was to analysis effect of giving mahkota dewa fruits (Phaleria macrocarpa) extract to epithelialization in incision wound of white rats (Rattus norvegicus). The method was randomized posted-only control group design. There were 30 male rats (Rattus norvegicus) grouped on control and treatment group. Control group divided into three groups (KK1, KK2, KK3) and also treatment group divided into three groups (KP1, KP2, KP3). Control group just given CMC 1% peroral without mahkota dewa fruits extract, the treatment group given mahkota dewa fruits extract 22.5 mg/kg body weight. The data was analyzed by Kruskall Wallis. Based on Kruskall Wallis test, obtained result that there was a significant difference (p<0.05) epithelialization variable with p value p = 0.000 between control and treatment group. And based on One-way Anova test, obtained result that there was a significant difference (p<0.05) with p value p =0.000 between control and treatment group. The conclusion of this research was giving mahkota dewa fruits (Phaleria macrocarpa) extract can increase epithelialization in incision wound of white rats (Rattus norvegicus). Keywords : Mahkota Dewa Fruits (Phaleria Macrocarpa) Extract, Epithelialization, Incision Wound

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Increased Brain Derived Neurothropic Factor in the cerebrum and cerebellum of Rattus norvegicus newborn with exposure to Mozart's music in default sequence compared with the reversed sequence and without exposure during gestation

Majalah Obstetri & Ginekologi ◽

10.20473/mog.v26i22018.67-73 ◽

2018 ◽

Vol 26 (2) ◽

pp. 67

Author(s):

Agustina Hidayati ◽

Hermanto Tri Joewono ◽

Widjiati Widjiati

Keyword(s):

Animal Models ◽

Rattus Norvegicus ◽

Control Group ◽

P Value ◽

Sequence Group ◽

Bdnf Expression ◽

Control Group Design ◽

Significant Difference ◽

Reverse Sequence ◽

And Control

Objectives: To analyze the differences in the expression of Brain Derived Neurothropic Factor (BDNF) in Rattus norvegicus cerebrum and cerebellum of newborn between those exposed to Mozart's music composition in default sequence, reverse sequence, and without exposure in the womb.Materials and Methods: Analytical laboratory experimental study with randomized post test only control group design using animal models Rattus norvegicus. The animal models were divided into three groups: control group without any exposure, the treatment groups with exposure to Mozart's music in default sequence and another group in reverse sequence since day 10 of gestation. We used a comparison test in the analysis of BDNF expression.Results: We found significant difference in BDNF expression with p value 0.004 (mean 8.98±1.31 default sequence group, 5.58±3.08 reverse sequence group, 6.80±1.95 control) in the cerebrum. We found significant difference of BDNF expression with p value 0.003 (mean 9.48±1.41 default sequence group, 6.02±3.25 reverse sequence group, 7.14±2.54 control) in the cerebellum. In cerebrum dan cerebellum we found significant difference between standard Mozart’s music and control (cere-brum p=0.018, cerebellum p=0.001), and we found significant difference between standard Mozart’s music and reverse Mozart’s music (cerebrum p=0.001, cerebellum p=0.008) and no significant difference in reverse Mozart and control (cerebrum p=0.264, cerebellum p=0.490)Conclusion: Sequence in Mozart’s music is very important in increase expression of BDNF.

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Optimalisasi Pertumbuhan Bayi dengan Baby Massage and Spa

Jurnal Kesehatan ◽

10.26630/jk.v10i2.1416 ◽

2019 ◽

Vol 10 (2) ◽

pp. 276

Author(s):

Yusari Asih ◽

I Gusti Ayu Mirah WS

Keyword(s):

Body Length ◽

Head Circumference ◽

Intervention Group ◽

Sampling Technique ◽

Holistic Approach ◽

The Body ◽

Control Group ◽

P Value ◽

Control Group Design ◽

Significant Difference

Baby Massage and Spa was a traditional effort that uses a holistic approach through comprehensive care using a combination of massage and water therapy methods that are carried out in an integrated manner to balance the body, mind, and feelings. Age 3-6 months is the right time for babies to do massage and spa. The purpose of this study was to determine the effect of baby massage and spa on the growth of infants aged 3-5 months in Pringsewu District in 2018. This study uses a quasi-experimental approach with research design pre and post-test with control group design. The purposive sampling technique was taken with a sample of 30 infants as the intervention group and 30 infants as the control group. Data were processed using computerization and analyzed using the T-Test. The results showed that there were significant differences between body weight, body length and baby head circumference in the pre and post-treatment. There was no significant difference in the average increase in infant weight between babies who were carried out by baby massage and spa with babies who were not carried out by baby massage and spa with p-value 0.116. There is a significant difference in the baby's body length and head circumference performed by baby massage and spa with those not carried out by baby massage and spa with p-value 0.000. Baby Massage and Spa has a significant effect on increasing the growth of babies aged 3-5 months.

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EFFECT OF FAMILY PSYCHOEDUCATION ON CAREGIVER SUPPORT IN THE TREATMENT OF PATIENTS WITH TYPE II DIABETES MELLITUS

Belitung Nursing Journal ◽

10.33546/bnj.342 ◽

2018 ◽

Vol 4 (1) ◽

pp. 112-119

Author(s):

Andika Siswoaribowo ◽

Mateus Sakundarno ◽

Muhammad Mu’in

Keyword(s):

Diabetes Mellitus ◽

T Test ◽

Control Group ◽

P Value ◽

Family Psychoeducation ◽

Control Group Design ◽

Caregiver Support ◽

Significant Difference ◽

Patients With Diabetes ◽

And Control

Background: Diabetes Mellitus is a chronic disease that requires treatment for long periods of time so it can cause physical and psychological problems for sufferers and families (caregiver). Caregiver's role is expected to provide support for people with diabetes mellitus. Family psychoeducation is a strategy that can be applied for caregiver in overcoming problems that arise during the treatment of patients with diabetes mellitus.Objective: This study aims to determine the effect of family psychoeducation on caregiver support in the treatment of patients with diabetes mellitus type II.Methods: This research used a quasi experiment with pre-test post-test control group design. A total of 46 caregivers and patients with diabetes mellitus were recruited puposively, with 23 respondents assigned in the experiment and control group. Caregiver support scale was used to measure caregiver support, and Hensarling Diabetes Family Support Scale (HDFSS) for measuring perception of patients toward the treatment of caregiver. Data were analyzed using paired t-test and independent t-test. Results: Findings showed there was an increase of caregiver knowledge from 5.39 to 9.09 and an improvement of caregiver treatment from 40.30 to 67.04 after given family psychoeducation. There was a significant difference of caregiver support in the experimental and control group with p-value <0.001 (<0.05).Conclusion: Family psychoeducation can increase caregiver support in the treatment of diabetes mellitus patients. The intervention can be one of nurses interventions in the empowerment of family in improving the treatment of chronic diseases, particularly in diabetes mellitus.

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THE COMPARISON OF THE EFFECT OF HONEY AND CHLORHEXIDINE IN PREVENTING VENTILATOR ASSOCIATED PNEUMONIA IN PATIENTS ON MECHANICAL VENTILATON

Belitung Nursing Journal ◽

10.33546/bnj.355 ◽

2018 ◽

Vol 4 (2) ◽

pp. 161-167

Author(s):

Syaukia Adini ◽

Bedjo Santoso ◽

Sarkum Sarkum ◽

Sudirman Sudirman

Keyword(s):

Oral Hygiene ◽

Control Group ◽

P Value ◽

Ventilator Associated Pneumonia ◽

Control Group Design ◽

Group Design ◽

Significant Difference ◽

Quasi Experimental ◽

The Mean ◽

Honey Solution

Background: Ventilator Associated Pneumonia (VAP) is one of the causes of infection in the hospital and the main cause of death due to nosocomial infection. The strategy to prevent VAP is by oral hygiene. Honey may be a good solution for oral hygiene.Objective: This study aims to compare the effect of the use of 20% honey solution and 0.2% chlorhexidine as oral hygiene on VAP prevention in patients on mechanical ventilation.Methods: This was a quasi-experimental study with posttest only control group design in an incentive care unit of a general hospital in Indonesia. Thirty respondents were selected using consecutive sampling, which 15 respondents assigned in a 20% honey group and 0.2% chlorhexidine group. Clinical Pulmonary Infection Score (CPIS) was used to measure Ventilator Associated Pneumonia. Data were analyzed using Independent t-test.Results: The mean of CPIS in the honey group was 3.33 and the chlorhexidine group was 3.53. Independent t-test showed p-value 0.618 (>0.05), which indicated that there was no significant difference of the effect of honey and chlorhexidine on VAP event.Conclusions: The 20% honey solution has the same effect with 0.2% chlorhexidine in preventing VAP events in patients on mechanical ventilation.

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Pengaruh Pendidikan Kesehatan Tentang Inspeksi Asam Asetat (IVA) Terhadap Motivasi Wus dalam Deteksi Kanker Serviks di Puskesmas Putri Ayu Jambi

Jurnal Ilmiah Universitas Batanghari Jambi ◽

10.33087/jiubj.v19i1.535 ◽

2019 ◽

Vol 19 (1) ◽

pp. 42

Author(s):

Hesty Hesty ◽

Rahmah Rahmah ◽

Nurfitriani Nurfitriani

Keyword(s):

Cervical Cancer ◽

Health Education ◽

Early Stage ◽

Sampling Technique ◽

World Health ◽

Control Group ◽

P Value ◽

Childbearing Age ◽

Control Group Design ◽

Experimental Group

World Health Organization (WHO), cervical cancer is the number two cause of death in the world for women from all existing cancer. Every two minutes a woman dies of this disease. This cancer attack at an early stage does not cause any symptoms. That is why, why does this type of cancer fall into the category of the silent killer. One of the promotive and preventive efforts in women of childbearing age as primary prevention can be through the provision of health education about cervical cancer using the Acetic Acid Inspection (IVA) method. The purpose of this study is to find out whether there is an influence of health education about IVA on WUS motivation in cervical cancer detection in health centers. Putri Ayu Jambi. This type of quantitative research with non randomized pretest-posttest with control group design, with purposive sampling technique totaling 22 WUS, 11 people as controls and 11 experimental people. The instrument in the form of questionnaires was analyzed by univariate and bivariate Paired Test t-test, which was conducted from April to July 2018. The results of the study revealed that of the 22 WUSs studied, the average motivation of the pretest in the experimental group was 41.1818 and the control group was 39.9091, while the results of the posttest of the experimental group after being given counseling and leaflets increased by 42.9091 with p-value 0.034. There is an influence of health education about IVA tests on WUS motivation in detecting cervical cancer. It is suggested that the Puskesmas can improve activities in planning disease control programs, especially carrying out counseling and counseling both in the puskesmas building and in the working area of the puskesmas by involving cadres in their environment so that WUS is moved to take the IVA examination.

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Short Communication: Effects of black soy phytoestrogens (Glycine soja) on elevated levels of estradiol in rat blood (Rattus novergicus) ovariectomy

Nusantara Bioscience ◽

10.13057/nusbiosci/n120110 ◽

2020 ◽

Vol 12 (1) ◽

Author(s):

Dwi Yuliawati ◽

WURI WIDI ASTUTI ◽

FITRI YUNIARTI

Keyword(s):

Group Treatment ◽

Rattus Norvegicus ◽

Glycine Soja ◽

Negative Control ◽

Female Rats ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

P Value ◽

Control Group Design ◽

Rat Blood

Abstract. Yuliawati D, Astuti WW, Yuniarti F. 2020. Effects of Black Soy phytoestrogens (Glycine soja) on elevated levels of estradiol in Rat Blood (Rattus norvegicus) ovariectomy. Nusantara Bioscience 12: 55-58. Menopause is the transition from productive to non-productive times due to reduced estrogen and progesterone and has an impact on improving cardiovascular problems. The fulfillment of estrogen needs in menopause can use a type of phytoestrogens from black soybeans. The purpose of research is to prove the administration of black soy extract (Glycine soja) can increase the levels of estradiol in the rat blood (Rattus norvegicus) ovariectomy. Design research uses post-test only control group design. Twenty-five female rats were randomly divided into 5 groups (n = 5 tails per group), negative controls, positive and 3 treatments. Ovariectomy was performed in a group of positive controls and treatment. Thirty-day post ovariectomy rats were given appropriate group treatment (negative control, positive, and treatment with the administration of black soy extract dose of 50, 100, 150 mg/200 g/day for 30 days). The estradiol test used the Enzyme-linked immunosorbent assay (ELISA). Anova's test results with a significant degree of α = 0.05 established that the p-value of 0.000 was less than α = 0.05, meaning there was a significant effect of giving black soy extract on increased levels of estradiol in the rat blood ovariectomy. Post - Hoc Tukey testing shows increased the highest estradiol levels in the dose treatment group 100 mg/200 g/day. This research proves that the administration of black soy extract (Glycine soja) can increase the levels of estradiol in the rat blood (Rattus norvegicus) ovariectomy.

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PENERAPAN METODEBERMAIN ANAK USIA DINI PADA KELOMPOK B UNTUK MENINGKATKAN KEMAMPUAN BERHITUNG PERMULAAN DI TK AL-FALAH

CERIA (Cerdas Energik Responsif Inovatif Adaptif) ◽

10.22460/ceria.v2i3.p84-91 ◽

2019 ◽

Vol 2 (3) ◽

pp. 84

Author(s):

Rina Septiadiningsih ◽

Euis Rohaety ◽

Lenny Nuraeni

Keyword(s):

Design Research ◽

Sensitive Period ◽

Control Group ◽

P Value ◽

Test Results ◽

Control Group Design ◽

Significant Difference ◽

Post Test ◽

The Right ◽

In The Beginning

Sensitive period is the right time to instill the basis of the importance of counting. In addition to counting skills that must be developed also pay attention to the application of learning used. However, in the learning process, teachers often forget the importance of playing methods. If too often with giving assignments the child will feel bored, therefore what is used is the method of playing. Play method is one method that can be used by teachers to teach the ability to count children in the beginning. Based on this background, the purpose of this study was to determine the effect of the use of playing methods on initial numeracy skills. This study used the Quasi Experiment method with the Nonequevalent Control Group Design research design. The results of the pre test showed that there were no significant differences in the experimental group and the control group with p-value> 0.05, which was 0.135> 0.05 which was tested by the two average test results. While the post-test results showed that the initial numeracy ability after the application of the playing method experienced a significant difference with the p-value <0.05, that is with the results of 0.0001 with the Mann-Whitney test. This method of playing can be used as a learning suggestion for educators to improve their ability to have an early start.

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PIJAT KOMBINASI ENDORFIN OKSITOSIN MEMPENGARUHI PRODUKSI ASI PADA IBU POST OPERASI SECTIO CAESAREA

Mahakam Midwifery Journal (MMJ) ◽

10.35963/midwifery.v5i1.138 ◽

2020 ◽

Vol 5 (1) ◽

pp. 51

Author(s):

Nursari Abdul Syukur ◽

Endah Wahyutri ◽

Erma Putri

Keyword(s):

Breast Milk ◽

Milk Production ◽

Sectio Caesarea ◽

Control Group ◽

P Value ◽

Control Group Design ◽

Breast Milk Production ◽

Significant Difference ◽

Quasi Experimental ◽

The Difference

Background: Breast milk spending is a very complex interaction between mechanics, nerves, and hormons. The decrease and production of breast milk production in the first days after delivery can be caused by a lack of stimulation of prolactin and oxytocin hormons. Caesarea section does not allow the mother to breastfeed her infant more flexibly, with adaptation to the pain, so she should be able to support the health worker thoroughly about the lactation process. Purpose: to know the difference of endorphin, oxytocin and combination effect on breast milk production in Postoperative section of caesarea section. Research design: quasi experimental design type post test only non equivalent control group design. The population in this study were all postoperative mothers caesarea section, 10 samples for each treatment. Group 1 : endorphin massage, group 2 (control) : the oxytocin massage, and group 3 : the combine massage. Results: Combination massage is the best method to produce the highest amount of ASI because the largest Sig p value is (1,000) where the level is significant <0.05. So H0 this study was rejected means that there is a significant difference between the production of breast milk from the combination massage compared with the two massage done separately. Conclusion: It is expected during childbirth the midwife can develop and implement independent non-pharmacological midwife actions to help increase milk production

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Pengaruh Pemberian Ekstrak Rumput Laut Cokelat (Sargassum duplicatum) terhadap Peningkatan Kadar Hematokrit pada Darah Tikus Putih Jantan Galur Wistar (Rattus norvegicus) Anemia yang di Induksi NaNO2

Oceana Biomedicina Journal ◽

10.30649/obj.v3i2.47 ◽

2020 ◽

Vol 3 (2) ◽

pp. 38

Author(s):

Ayu Mayang A. P. ◽

Liliawanti Liliawanti ◽

Wachjudi Kurnia

Keyword(s):

Rattus Norvegicus ◽

White Male ◽

Brown Seaweed ◽

Negative Control ◽

Laboratory Method ◽

The Body ◽

Male Rats ◽

Control Group ◽

Control Group Design ◽

Significant Difference

Anemia is still a high prevalence disease in Indonesia, caused by a lack of iron in the body. The components from brown seaweed such as cobalamin (vitamin B12), pyridoxine (vitamin B6), chlorophyll and iron (Fe) can increase red blood cells in the body. The purpose of this research was to determine the effect of brown seaweed (Sargassum duplicatum) which can increase hematocrit levels in the blood of white male wistar strain rat (Rattus norvegicus) suffered from anemia in induction NaNO2. This research uses an experimental laboratory method with a post test only control group design. The samples were 30 male rats divided into 3 treatment groups, the negative control group (K0) without treatment, the positive control (K1) be treated anemia given NaNO2 for 18 days without Sargassum duplicatum extract and treatment group (K2) treated with anemia given NaNO2 for 18 days without Sargassum duplicatum extract at a dose of 2,45gr / kgBB. On the 40th day, a blood sample was taken, then measured the hematocrit level. The results showed that the mean number of blood hematocrit levels in K2 was higher than K1, it showed a significant difference descriptively. Based on the Kruskal-Wallis test, a value of 0.260 was obtained that p > 0,05 showed that there were no statistically significant differences between groups of experimental animals. From the results of this research, the conclusion is the administration of brown seaweed extract (Sargassum duplicatum) descriptively increased, but not statistically significant. Keywords : Anemia, hematocrit, Sargassum duplicatum, NaNO2

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