scholarly journals Therapeutic tactics with violations of the menstrual cycle caused by ovulative dysfunction

2018 ◽  
pp. 40-44
Author(s):  
G. B. Dikke
Keyword(s):  
Menstrual Cycle ◽  
Protective Effect ◽  
Active Form ◽  
Biologically Active ◽  
Polycystic Ovaries ◽  
The Metabolic Syndrome ◽  
Ovulatory Dysfunction ◽  
Cyclic Production ◽  
Cycle Disorders ◽  
Positive Effect

Purpose: presentation of modern data on the pathogenesis of menstrual cycle disorders (NMCs) associated with ovulatory dysfunction, and the effectiveness of their treatment with medication.Basic provisions. It is shown that NMC is the result of violations of the cyclic production of gonadotropic and sex hormones. Among the hormonal drugs for the treatment of NMCs, combined estrogen-progestational oral contraceptives (COCs), especially those with drospirenone (DRSP), are advantageous. The introduction of calcium levomefolate - biologically active form of folatein the composition of COCs containing 30 μg of ethinylestradiol and DRSP has a potentiating effect associated with their noncontraceptive effects due to antiandrogenic and antimineralocorticoid activity. It was shown that calcium levomefolate, which participates in human body metabolism, as a component of COC, has a protective effect on the cardiovascular system (due to a decrease in the level of homocysteinemia) in the syndrome of polycystic ovaries, contributes to reducing obesity.Conclusion. The combination of 30EE/DRSP promotes the normalization of the menstrual cycle and has non-contraceptive effects associated with antiandrogenic and antimineralcorticoid action, and fortification with calcium levomefolate also has a positive effect on the hormonal profile and lipid metabolism, has a protective effect on cardiovascular diseases in women with the metabolic syndrome in polycystic ovaries, contributes to the normalization of weight. 

Processing and Characterization of Recombinant von Willebrand Factor Expressed in Different Cell Types Using a Vaccinia Virus Vector

1992 ◽  
Vol 67 (01) ◽  
pp. 154-160 ◽  
Author(s):  
P Meulien ◽  
M Nishino ◽  
C Mazurier ◽  
K Dott ◽  
G Piétu ◽  
...  
Keyword(s):  
Vaccinia Virus ◽  
Von Willebrand Factor ◽  
Human Fibroblasts ◽  
Active Form ◽  
Cell Types ◽  
Biologically Active ◽  
L Cells ◽  
Von Willebrand ◽  
Different Cell Types ◽  
Willebrand Factor

SummaryThe cloning of the cDNA encoding von Willebrand factor (vWF) has revealed that it is synthesized as a large precursor (pre-pro-vWF) molecule and it is now clear that the prosequence or vWAgll is responsible for the intracellular multimerization of vWF. We have cloned the complete vWF cDNA and expressed it using a recombinant vaccinia virus as vector. We have characterized the structure and function of the recombinant vWF (rvWF) secreted from five different cell types: baby hamster kidney (BHK), Chinese hamster ovary (CHO), human fibroblasts (143B), mouse fibroblasts (L) and primary embryonic chicken cells. Forty-eight hours after infection, the quantity of vWF antigen found in the cell supernatant varied from 3 to 12 U/dl depending on the cell type. By SDS-agarose gel electrophoresis, the percentage of high molecular weight forms of vWF varied from 39 to 49% relative to normal plasma for BHK, CHO, 143B and chicken cells but was less than 10% for L cells. In all cell types, the two anodic subbands of each multimer were missing. The two cathodic subbands were easily detected only in BHK and L cells. By SDS-PAGE of reduced samples, pro-vWF was present in similar quantity to the fully processed vWF subunit in L cells, present in moderate amounts in BHK and CHO and in very low amounts in 143B and chicken cells. rvWF from all cells bound to collagen and to platelets in the presence of ristocetin, the latter showing a high correlation between binding efficiency and degree of multimerization. rvWF from all cells was also shown to bind to purified FVIII and in this case binding appeared to be independent of the degree of multimerization. We conclude that whereas vWF is naturally synthesized only by endothelial cells and megakaryocytes, it can be expressed in a biologically active form from various other cell types.

Innovative approaches to the correction of chronotrope status of pregnant and lactating women

2016 ◽  
pp. 37-40
Author(s):  
S.I. Zhuk ◽  
◽  
K.K. Bondarenko ◽  
Keyword(s):  
Folic Acid ◽  
Neural Tube ◽  
Active Form ◽  
Biologically Active ◽  
Lactating Women ◽  
Pathological Conditions ◽  
Prevention And Treatment ◽  
The Impact ◽  
High Bioavailability ◽  
Key Words Mthfr

Most recent studies show the impact of violations in the metabolism of folate and metin period in the pathogenesis of neural tube defects (NTD) of the fetus. Metafolin has a number of advantages, which primarily includes direct intake of substances in biologically active form and the optimum effect, even in the case when the patient homozygote and/or heterozygote genotype 677С T polymorphism in MTHFR. With the aim of prevention and treatment of various pathological conditions related to folate deficiency during pregnancy, it is advisable to apply vitamin-mineral complexes, containing metafolin - active form of folate with high bioavailability. Key words: MTHFR, metafolin, folic acid, pregnancy.

scholarly journals Folate in Red Rhapsody Strawberry—Content and Storage Stability.

Proceedings ◽  
2020 ◽  
Vol 70 (1) ◽  
pp. 47
Author(s):  
Julius Rami ◽  
Caroline Dumler ◽  
Nadine Weber ◽  
Michael Rychlik ◽  
Gabriele Netzel ◽  
...  
Keyword(s):  
Storage Stability ◽  
Active Form ◽  
Food Composition ◽  
Biologically Active ◽  
Stable Isotope Dilution ◽  
Isotope Dilution Assay ◽  
C Storage ◽  
Vitamin B9 ◽  
Adults And Children ◽  
And Storage

Folate (Vitamin B9) is critical for a range of biological functions in adults and children, including DNA, protein and neurotransmitter synthesis. It is also essential for the healthy development of the fetus in early pregnancy and for the prevention of neural tube defects, such as spina bifida. Strawberries are considered a tasty and healthy fruit consumed all over the world and may potentially be an important dietary source of natural folates. However, the relative importance of strawberry as a dietary source will depend on the total folate concentration, vitamer profile, storage stability and bioavailability to humans. Red Rhapsody, an important commercial strawberry cultivar in Australia, was screened for its folate content and storage stability by stable isotope dilution assay (SIDA). Total folate content ranged from 90–118 μg/100 g fresh weight (fw), which was well above the value in the Australian Food Composition Database (39 μg/100 g fw). 5-Methyltetrahydrofolate, the biologically active form in humans, was the principal vitamer present. Furthermore, folate remained relatively stable during refrigerated (4 °C) storage (loss of only 28% after 14 days of storage). This information is relevant for consumers since the inherent perishability of strawberry fruit makes refrigerated storage common practice in Australian households.

The importance of linkers in the structure of PSMA ligands

2021 ◽  
Vol 28 ◽  
Author(s):  
Anastasia A. Uspenskaya ◽  
Ekaterina A. Nimenko ◽  
Aleksei E. Machulkin ◽  
Elena K. Beloglazkina ◽  
Alexander G. Majouga
Keyword(s):  
Prostate Cancer ◽  
Solid Phase ◽  
Biologically Active ◽  
Modern World ◽  
Synthesis Methods ◽  
Amino Acid Residues ◽  
Ongoing Research ◽  
Phase Synthesis ◽  
Diagnose Prostate Cancer ◽  
Positive Effect

: Cancer is one of the leading social problems of the modern world. Today prostate cancer is the second leading cause of cancer deaths among men. Targeted drug delivery is widely used to treat and diagnose prostate cancer. Conjugates selectively binding to prostate specific membrane antigen based on urea ligands are being actively developed against this disease. The linker has a significant influence on the biological activity of such conjugates. The linker performs a large number of functions, and its modification is one of the key methods of creating the best pharmacological profile. This review aims to discuss and analyze the main approaches to the method of introduction and synthesis of linkers for this type of conjugates without a description of the influence of biologically active molecules, as well as to establish the key modification methods that have a significant role on the structure-activity relationship. For this purpose, a review of the current scientific literature was performed, both for the conjugates under development and for those already undergoing clinical trials. It was found that the optimal structure is a linker containing an aliphatic fragment near the vector-molecule (n(CH2) = 3-6), followed by a polypeptide chain consisting of 2 to 4 amino acid residues. The presence of a Phe-Phe dipeptide chain or the introduction of negatively charged groups also has a positive effect. Ongoing research in this field helps to establish the accurate effect of each linker fragment, and the development of solid-phase synthesis methods makes it much easier to achieve this goal.

scholarly journals Enriching the diets of young cattle with a natural biologically active feed additive

2021 ◽  
Vol 22 (1) ◽  
pp. 94-103
Author(s):  
I. Yu. Kuzmina ◽  
L. S. Ignatovich
Keyword(s):  
Live Weight ◽  
Biologically Active ◽  
Raw Material ◽  
Wild Plants ◽  
Feed Additive ◽  
Feed Conversion ◽  
Holstein Bulls ◽  
Young Cattle ◽  
Feed Protein ◽  
Positive Effect

On the basis of the "Komarova" farm (Magadan, Magadan region), the effect of introducing a new non-traditional component feed additive of plant origin into the diets of young cattle was studied. The research was carried out during the stall period. For the experiment there were taken four groups of animals (14-16 months of age) divided at random in accordance with the genotype into control and experimental groups of ten animals each. The first two groups (control, experiment) included purebred Holstein bulls, the other two – hybrids of the 1st generation (1/2 Holstein × 1/2 Herefords). For the experimental groups, the main diet was supplemented with a component feed additive consisting of cedar elfin (Mountain pine) flour and lichens (alpine cladonia (Cladonia alpestris), Icelandic cetraria (Cetraria islandica)). The results of the experiment has shown that the feed additive, consisting of 120 g of cedar elfin needle flour and 50 g of lichen flour (80% of Cladonia alpestris and 20% of Cetraria islandica), has a positive effect on the intensification of metabolic processes in the animals. The digestibility of crude protein increased by 1.29-4,19 g (1.7-5.6 %); that of the organic matter – by 6.65-8.90 g (11.0-14.3 %); NFE (nitrogen-free extracts) – by 7.80-9.18 g (14.14-15.70 %). An increase in the digestibility of feed nutrients had a positive effect on an increase in live weight gain by 0.60-3.0 g (1.35-5.93 %). An increase in feed conversion was 0.24-6.8 MJ (0.20-6.74 %); feed protein – by 0.02-0.09 g (1.22-6.17 %). Crossbred bulls turned out to be more responsive to changes in the ration of feeding and showed better results in all studied parameters. The effectiveness of using the additive is due to the fact that the raw material for the production is obtained from wild plants growing in nature in sufficiently large volumes. They do not require significant costs for harvesting and preparation for feeding.

Digestibility of nutrients and energy metabolism in lactating cows when sapropel is included in the ration

2021 ◽  
pp. 22-27
Author(s):  
L. P. Yarmots ◽  
G. A. Yarmots ◽  
A. E. Belenkaya ◽  
M. O. Smyshlyaeva
Keyword(s):  
Energy Metabolism ◽  
Milk Fat ◽  
Animal Husbandry ◽  
Feed Additives ◽  
The Body ◽  
Biologically Active ◽  
Main Trend ◽  
Control Group ◽  
Lactating Cows ◽  
Positive Effect

Unbalanced mineral and vitamin nutrition of lactating cows can be a critical factor in the realization of their productive potential. The development and introduction into production of inexpensive, but effective feed additives is the main trend of improving the feeding of modern animal husbandry. Under the conditions of the Tyumen region such natural raw materials can be sapropels, which successfully combine a variety of biologically active substances. The purpose of the research was to study the digestibility of nutrients and energy metabolism in lactating cows when sapropel is included in the ration. Scientifi c and economic experiment in the study of the effectiveness of feeding sapropel lake Nepryak has been carried out in the training and experimental farm of the State Northern Trans-Urals Agrarian University. The animals of the control group have been fed the main diet. Cows of the 1st and 2nd experimental groups have been fed 300 and 500 g of sapropel per head/day, respectively, in addition to the main ration. It has been found as a result of research that the introduction of sapropel into the ration of cows has a positive effect on the digestibility of nutrients. The digestibility coefficients were higher in the animals of the 1st and 2nd experimental groups compared with the cows of the control group. Cows from the experimental groups digested all the organic substances of the ration better. During the experiment period cows of the 1st and 2nd experimental groups in contrast to the control group had higher milk yield by 11,31 and 10,38 %, respectively, milk protein yield by 10,56 and 5,95 %, milk fat yield by 17,18 and 12,99 %. It has been established that the introduction of sapropel into the ration of cows has the positive effect on the digestibility of nutrients, contributes to the increase of milk productivity, normalizes the metabolism in the body.

The joint use of enzyme drugs and lecithin in the rearing of broiler chickens

2021 ◽  
pp. 41-46
Author(s):  
B. S. Kaloev ◽  
M. O. Ibragimov ◽  
M. M. Shagaipov
Keyword(s):  
Broiler Chickens ◽  
Live Weight ◽  
Biologically Active ◽  
Feed Consumption ◽  
Control Group ◽  
Growing Period ◽  
Production Experiment ◽  
Productive Traits ◽  
Positive Effect ◽  
Experimental Group

The reliability of the results obtained in scientific and economic experiments on the study of the effectiveness of the use of various feed components, including biologically active substances in the rearing of broilers needs to be confi rmed in production conditions. The purpose of the research was to study the joint use of enzyme drugs and lecithin in the rearing of broiler chickens. The results of production experiment on the study of the effectiveness of the joint inclusion of enzyme drugs Sunzyme and Sunfyse 5000 as well as lecithin in the diet of broiler chickens of the cross Ross-308 have been presented in the article. Production tests have been carried out under the conditions of the breeding farm “Achkhoy– Martanovsky” in the Chechen Republic. The duration of the experiment was 45 days. For research on the principle of analog groups two groups have been formed: a control group and an experimental group of 1000 heads in each. The broilers of the control group have been fed compound feed prepared in accordance with the growing period and consisting mainly of grain components of local production. The broilers of the experimental group have been fed the same compound feed, but with the addition of two enzyme drugs Sanzyme and Sanfyse 5000 (100 g/t of feed) as well as lecithin (10 g/kg of feed). The results of the production experiment fully confi rmed the data obtained in the scientific and economic experiment. The positive effect of the joint use of the studied enzyme drugs and lecithin has been manifested in all the studied productive traits. In particular, the increase in live weight of broilers increased by 12,1 %, slaughter yield by 3,5 %, the yield of carcasses of the 1st category by 3,0 %. The main economic indicators also found their confirmation: feed consumption per unit of growth decreased by 11,7 %, additional profit was received in the amount of 27308,2 rubles, the level of profitability of broiler rearing increased by 18,4 %.

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