GCMS Analysis of Bioactives of Piper betle Linn. Leaf

2020 ◽  
Vol 16 (1) ◽  
pp. 24-32
Author(s):  
Sapna Saini ◽  
Sanju Nanda ◽  
Anju Dhiman
Keyword(s):  
Bioactive Compounds ◽  
Quantitative Estimation ◽  
Piper Betle ◽  
Pharmacological Activities ◽  
Constant Flow Rate ◽  
Traditional System ◽  
Head Space ◽  
Ms Analysis ◽  
Space Method ◽  
Good Percentage

Background: Piper betle Linn. (Piperaceae) is commonly known as “Green gold of India” due to its high commercial value. It is a Vedic plant used in various traditional system of medicine v.iz. Ayurveda system of medicine, Yunani system of medicine attributed to various pharmacological activities v.i.z. antioxidant, anti-inflammatory, analgesic, anti-fertility, antimutagenic etc. Objectives: The main objective of the present study is quantitative estimation of the various bioactive compounds present in leaf of P. betle L. (Bangla variety) by GC-MS analysis using head space method. Material and Methods: The vapor sample of leaf of P. betle L. was prepared by head space method for GCMS analysis. GCMS analysis was carried out using high resolution Thermo scientific TSQ 8000 Gas Chromatograph-Mass Spectrometer. Helium is used as carrier gas (99.999 %) with a constant flow rate of 1 ml/min. Total run time was 29.10 min. Results: GC-MS analysis revealed the presence of 16 bioactive compounds in leaf of P. betle L. Eugenol (29.13%), Bakuchiol (11.79%) and α-linalool (14.88%) are the chief bioactive compounds of leaf. Leaf has been also reported to contain a good percentage of various terpenoids like β- caryophyllene (7.69), α-muurolene (4.19), α-bisabolene (0.98), etc. Conclusion: From GC-MS analysis results it can be concluded that leaf of P. betle L. is a reservoir of valuable phenolic and susquiterpene compounds. Eugenol chief biomarker compound present leaf is a versatile remarkable molecule. Due to number of phytoconstituents P. betle L. leaf is recommended as plant of phytopharmaceutical importance.

scholarly journals GC–MS study on ethanolic extract of Vetiveria lawsonii root

2016 ◽  
Vol 5 (3) ◽  
pp. 108-111
Author(s):  
P Ramachandran ◽  
◽  
Viji Saral Elezabeth D Elezabeth D ◽  
Keyword(s):  
Mass Spectrometer ◽  
Bioactive Compounds ◽  
Ethanolic Extract ◽  
Pharmacological Activities ◽  
Ms Analysis

The present study was focused to scrutinize the presence of phytoconstituents in the ethanolic extract of Vetiveria lawsonii root using GC-MS. The GC-MS analysis of Vetiveria lawsonii root was performed using Agilent 6890-JEOL GC-Mate-II Mass Spectrometer. The result of the study showed the presence of six bioactive compounds in the ethanolic extract. On account of this investigation, we can conclude that the ethanolic extract of Vetiveria lawsonii root shows the presence of 6 bioactive phytoconstituents. This might be the cause for the various pharmacological activities of the plant.

scholarly journals GC-MS Analysis of Bioactive Compounds and Phytochemical Evaluation of the Ethanolic Extract of Gomphrena globosa L. Flowers

2020 ◽  
Vol 10 (2) ◽  
pp. 53-58
Author(s):  
Azmat Unnisa Esmat ◽  
Sirisha Mittapally ◽  
Safiya Begum
Keyword(s):  
Bioactive Compounds ◽  
Ethanolic Extract ◽  
Pharmacological Activities ◽  
Ms Analysis ◽  
Gomphrena Globosa ◽  
Whole Plant ◽  
Docosanoic Acid ◽  
Phytochemical Compounds ◽  
Phytochemical Constituents ◽  
Anti Inflammatory

Gomphrena globosa (L.) has high medicinal values. All parts are been used as folk medicines. The extract of whole plant / flowers has shown different phytochemical constituents with various clinical properties. These phytochemicals recognised are responsible for various activities such as anti-inflammatory, anticancer, antibacterial, analgesic and cytotoxic. The ethanolic extract of Gomphrena globosa L. flowers of biochemical test indicates the presence of sterols, triterpenoid, tannins, phenols and flavonoids, and on GC-MS analysis it has shown 11 phytochemical compounds with different pharmacological activities. The major bioactive compounds are Docosanoic Acid, Docosyl Ester (25.404%) and Hexatriacontane (24.324%), has proven anti-inflammatory activity. Keywords: Gomphrena globose L., phytochemical constituents, GC-MS and anti-inflammatory.

Mentha arvensis (Pudina): A Review Based upon its Medicinal Properties

2021 ◽  
pp. 143-148
Author(s):  
Shifali Thakur ◽  
Bhawna Walia ◽  
Gitika Chaudhary
Keyword(s):  
Bioactive Compounds ◽  
Primary Source ◽  
General Description ◽  
Mentha Arvensis ◽  
Pharmacological Activities ◽  
Medicinal Uses ◽  
Traditional System ◽  
Phytochemical Compounds ◽  
Medicinal Properties ◽  
Flavoring Agent

Since the origin in life, plants have remained the primary source of food, shelter and various remedial approaches. Plants are being in use for treating various kind of diseases across the world. Mentha arvensis linn. is a medicinal plant belonging to the Lamiaceae family. It is utilized as a household remedy, food seasoner and for many other industrial purposes. Mentha arvensis is traditionally used in patients with hypertension and ischemic heart disease. The plant species are well recognized for their folk medicinal uses, especially to treat cold, fever, digestive and cardiovascular disorders. The leaves of Mentha arvensis are recorded to possess potent therapeutic values. Leaves of plant also possess aromatic fragrance and are utilized as a flavoring agent. The leaves are utilized for the treatment of liver disease, spleen, asthma and jaundice. Various bioactive compounds isolated from this plant are terpenoids, alcohols, rosmarinic acids and phenolics. These bioactive compounds have many pharmacological properties such as antimicrobial, hepatoprotective, cardioprotective, antioxidant, antiulcer, immunomodulatory, antifertility and anti-tumor. These pharmacological activities of the Mentha arvensis plant are also mentioned in the literature of ayurveda, unani and other traditional system of medicine. The present review is summarizing the general description, phytochemical compounds, traditional view, modern view and pharmacological activities of the Mentha arvensis plant.

scholarly journals Phytochemical Analysis of Caesalpinia pulcherrima (L.) Sw. Leaf Extract Using GC-MS Analysis

2017 ◽  
Vol 9 (2) ◽  
Author(s):  
Saravanan P.
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Bioactive Compounds ◽  
Leaf Extract ◽  
Folk Medicine ◽  
Phytochemical Analysis ◽  
Pharmacological Activities ◽  
Malarial Infection ◽  
Ms Analysis ◽  
Soxhlet Apparatus

Most of the worlds populations residing in developing countries depend on alternative medicine and use of plant ingredients. Caesalpinia pulcherrima (Fabaceae) is used in folk medicine for the treatment of various diseases including asthma, bronchitis, cholera, diarrhea, dysentery and malarial infection. The aim of the study to identified bioactive compounds presence in the chloroform leaf extract of Caesalpinia pulcherrima (L.) Sw. using Gas Chromatography and Mass Spectrometry (GC-MS) analysis. The leaf extraction was done by Soxhlet apparatus method. The GC-MS analysis has shown the presence of thirteen compounds in the chloroform leaf extract of Caesalpinia pulcherrima (L.) Sw. The identified bioactive compounds from this plant have been known to possess pharmacological activities.

scholarly journals Ethnobotanical and Pharmacological Importance of Western Himalayan Fir Abies pindrow (Royle ex D. Don) Royle: A Review

2019 ◽  
pp. 1-14
Author(s):  
Dwaipayan Sinha
Keyword(s):  
Bioactive Compounds ◽  
Indian Subcontinent ◽  
Chemical Constituents ◽  
Literature Survey ◽  
Extensive Literature ◽  
Bioactive Constituents ◽  
Pharmacological Activities ◽  
Traditional System ◽  
Bladder Diseases ◽  
Abies Pindrow

Aims: Abies pindrow (Royle ex D. Don) Royle., colloquially known as the Western Himalayan fir, is a conifer that grows along the Himalayan mountains from Afghanistan to Nepal. The plant is extensively used by locals for the treatments of various ailments. Thus based on the available reports about its importance in traditional system of medicine, an attempt has been made to review this species in context of its medicinal and pharmaceutical importance. Study Design: The review article has been designed based on literature survey. The article is grossly divided into four broad phases consisting of compiled information of Abies pindrow: (1) Ethnobotanical information (2) Chemical Constituents (3) Pharmacological activities (4) Discussion mainly highlighting the mode of action of the bioactive compounds in relation to its pharmacological activity. Methodology: Extensive literature search have been performed in the web using PubMed, PubMed Central, google scholar as search platform. Efforts have also been taken to compile information from research papers and review articles not more than 10 years old. Information from old literatures were preferably avoided unless found to be very relevant to the subject. Results: The literature survey revealed that the plant has been used to treat cough and cold, diabetes, cataract and bladder diseases. The plant is also considered to be carminative, astringent, antispasmodic, diuretic, tonic and anti-inflammatory. The plant is also rich in terpenes and terpene alcohols. Testing for antidiabetic, neuroprotective, anticataract and antixiolytic activities have been promising and the bioactive constituents and efforts are made to interpret the possible mechanism of action of bioactive compounds in bringing about the pharmacological activities. Conclusion: It is concluded that the plant can be explored and bioprospected for an affordable source of drug and nutraceutical for better management of health related issues of people of Indian subcontinent.

FTIR, GC-MS and HPLC analysis of Baliospermum montanum (Willd.) Muell. Arg.

2020 ◽  
Vol 11 (4) ◽  
pp. 5059-5066
Author(s):  
Sushma B K ◽  
Raveesha H R
Keyword(s):  
Acetic Acid ◽  
Quantitative Estimation ◽  
Chemical Constituents ◽  
Standard Procedure ◽  
Naphthalene Acetic Acid ◽  
Root Extract ◽  
Pentanoic Acid ◽  
Trimethylsilyl Ester ◽  
Isolation And Characterization ◽  
Ms Analysis

The present work is aimed to determine the chemical constituents in Baliospermum montanum methanolic extracts. An in vitro regenerated procedure was developed for the induction of callus from stem explant cultured on Murashige and Skoog (MS) medium fortified with various concentration and permutations of 2, 4-dichloro phenoxy acetic acid, 1-naphthalene acetic acid, 6-benzyl amino purine and gibberellic acid. FTIR & GC-MS analysis was done according to standard procedure. The quantitative estimation of β-sitosterol was done by HPLC method. Maximum fresh and dry weight of callus was estimated in the combination of GA3 (0.5 mg/L) + NAA (2 mg/L) compared to other concentration. The FTIR analysis showed various functional compounds with different characteristic peak values in the extracts. Major bioactive constituents were recognized in the GC-MS analysis. Root extract revealed the existence of 1-hexadecanol, pentanoic acid, 2-(aminooxy)- and 1-hexacosanol. Leaf extract showed the presence of propanoic acid, 2-oxo-, trimethylsilyl ester, 9,12-octadecadienoic acid (z,z)-, trimethylsilyl ester, docosane, 1,22-dibromo- and pentatriacontane. Stem and stem derived callus exhibit the presence of 1,6,3,4-dihydro-2-deoxy-beta-d-lyxo-hexopyranose, n-hexadecanoic acid and pentanoic acid, 2-(aminooxy). The methanolic extract of leaf exhibited 0.2149 % of β-sitosterol content. There were no peaks observed in the root, stem and stem derived callus. Further studies are necessary for the isolation and characterization of bioactive compounds from B. montanum.

In-vitro bactericidal activity of a novel plant source Plumeria pudica against some human and fish pathogenic bacteria

2020 ◽  
Vol 17 ◽  
Author(s):  
Shubhaisi Das ◽  
Sunanda Burman ◽  
Goutam Chandra
Keyword(s):  
Ethyl Acetate ◽  
Bioactive Compounds ◽  
Pathogenic Bacteria ◽  
Ethyl Acetate Extract ◽  
Positive Control ◽  
Leaf Extracts ◽  
Ms Analysis ◽  
Ft Ir ◽  
Aldehyde Groups

Background: The only remedy for up surging problem of antibiotic resistance is the discovery of antibacterial agents of natural origin. Objective: The present study was aimed at finding antibacterial potential of crude and solvent extracts of mature leaves of Plumeria pudica. Methods: Antibacterial activity of three different solvent extracts were evaluated in four human and four fish pathogenic bacteria by measuring the zone of inhibition and determining Minimum Inhibitory Concentration and Minimum Bactericidal Concentration values. Standard antibiotics were used as positive control. Preliminary phytochemical screening of most effective extract i.e., ethyl acetate extract, Fourier Transform Infra Red analysis and GC-MS analysis of the Thin Layer Chromatographic (TLC) fraction of ethyl acetate extract were done meticulously. All experiments were done thrice and analyzed statistically. Results: Crude leaf extracts and solvent extracts caused good inhibition of bacterial growth in all selected bacteria. Ethyl acetate extract showed highest inhibition zones in all tested strains with maximum inhibition (19.50±0.29 mm) in Escherichia coli (MTCC 739). MBC/MIC of the extracts indicated that all three solvent extracts were bactericidal. Preliminary phytochemical tests revealed the presence of tannins, steroids and alkaloids and FT-IR analysis revealed presence of many functional groups namely alcoholic, amide, amine salt and aldehyde groups. From the GC-MS analysis of TLC fraction of ethyl acetate extract five different bioactive compounds e.g., 2,4-ditert –butylphenyl 5-hydroxypentanoate, Oxalic acid; allyl nonyl ester, 7,9-Ditert-butyl-1-oxaspiro(4,5)deca-6,9-diene-2,8-dione, Dibutyl phthalate and 2,3,5,8-tetramethyl-decane were identified. Conclusion: Leaf extracts of P. pudica contain bioactive compounds that can be used as broad spectrum bactericidal agent.

Flavones and Their Analogues as Bioactive Compounds – An Overview

2019 ◽  
Vol 16 (4) ◽  
pp. 377-391 ◽  
Author(s):  
B.S. Jayashree ◽  
H. Venkatachalam ◽  
Sanchari Basu Mallik
Keyword(s):  
Biological Activity ◽  
Free Radical ◽  
Bioactive Compounds ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Cost Effective ◽  
Free Radical Scavenging ◽  
Polyphenolic Compounds ◽  
Pharmacological Activities ◽  
Poor Solubility

Flavonoids constitute a large group of polyphenolic compounds that are known to have antioxidant properties, through their free radical scavenging abilities. They possess a chromone (γ- benzopyrone) moiety, responsible for eliciting many pharmacological activities. Even though, natural flavonoids are highly potent, owing to their poor solubility, they are less used. Therefore, attempts have been made to improve their stability, solubility, efficacy and kinetics by introducing various substituents on the flavone ring. For nearly the last two decades, flavones were synthesized in our laboratory by simple, convenient and cost-effective methods, with the knowledge of both synthetic and semi-synthetic chemistry. In this direction, it was considered worthwhile to present an overview on the synthesized flavonoids. This review creates a platform for highlighting various modifications done on the flavone system along with their biological activity.

scholarly journals Cyanobacteria—From the Oceans to the Potential Biotechnological and Biomedical Applications

Marine Drugs ◽  
2021 ◽  
Vol 19 (5) ◽  
pp. 241
Author(s):  
Shaden A. M. Khalifa ◽  
Eslam S. Shedid ◽  
Essa M. Saied ◽  
Amir Reza Jassbi ◽  
Fatemeh H. Jamebozorgi ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Biomedical Applications ◽  
Large Scale ◽  
Biological Activities ◽  
Scale Production ◽  
Pharmacological Activities ◽  
Large Scale Production ◽  
Marine Products ◽  
Hiv 1 ◽  
Successful Phase

Cyanobacteria are photosynthetic prokaryotic organisms which represent a significant source of novel, bioactive, secondary metabolites, and they are also considered an abundant source of bioactive compounds/drugs, such as dolastatin, cryptophycin 1, curacin toyocamycin, phytoalexin, cyanovirin-N and phycocyanin. Some of these compounds have displayed promising results in successful Phase I, II, III and IV clinical trials. Additionally, the cyanobacterial compounds applied to medical research have demonstrated an exciting future with great potential to be developed into new medicines. Most of these compounds have exhibited strong pharmacological activities, including neurotoxicity, cytotoxicity and antiviral activity against HCMV, HSV-1, HHV-6 and HIV-1, so these metabolites could be promising candidates for COVID-19 treatment. Therefore, the effective large-scale production of natural marine products through synthesis is important for resolving the existing issues associated with chemical isolation, including small yields, and may be necessary to better investigate their biological activities. Herein, we highlight the total synthesized and stereochemical determinations of the cyanobacterial bioactive compounds. Furthermore, this review primarily focuses on the biotechnological applications of cyanobacteria, including applications as cosmetics, food supplements, and the nanobiotechnological applications of cyanobacterial bioactive compounds in potential medicinal applications for various human diseases are discussed.

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