UJI AKTIVITAS PENURUNAN KADAR ASAM URAT EKSTRAK ETANOL BUAH PINANG YAKI (Areca vestiaria) PADA TIKUS  PUTIH GALUR WISTAR (Rattus norvegicus)  YANG DIINDUKSI KALIUM OKSONAT

ABSTRACTAn unhealthy diet in a high society protein, especially animal protein that contains a lot of purine, causes hyperuricemia (excess uric acid) to increase. The use of allopurinol that is too frequent or excessive can cause dangerous side effects. Based on these things, it is necessary to do research on antihyperuricemia substances that have high effectiveness and guaranted safety. This study aims to determine the potential for decreasing uric acid levels of areca nut fruit. This type of research as laboratory experiments using a Completely Randomized Design (CRD), with 5 types of treatment each treatment with 3 replications. Group 1 as a negative control was given induction of potassium oxonate, Group 2 as a positive control was given induction of potassium oxonate and allopurinol. Group 3-5 was trated with betel nut extract with different doses of 3,6 mg; 7,2 mg and 14,4 mg. Based on the results of the ANOVA statistical test, serum uric acid levels were significantly different from p = 0,002 (< 0,05). The results of the ethanol extracts of pinangyaki fruit at doses of 3,6 mg / 200gr BW, 7.2mg / 200gr BW and 14,4mg / 200gr BW can reduce serum uric acid levels by anova statistical test with a confidence level of 95%. Keywords: Yaki Areca Fruit, Anthihiperurisemia, Anova ABSTRAKPola makan yang tidak sehat dalam masyarakat yang berprotein tinggi,terutama protein hewani yang banyak mengandung purin, menyebabkan penyakit hiperurisemia (kelebihan asam urat) semakin meningkat. Penggunaan allopurinol yang terlalu sering atau berlebihan dapat menimbulkan efek samping yang berbahaya. Berdasarkan hal-hal tersebut, perlu dilakukan penelitian mengenai zat antihiperurisemia yang memiliki efektivitas tinggi dan terjamin keamanannya . Penelitian ini bertujuan untuk mengetahui potensi aktivitas penurunan kadar asam urat dari Buah Pinang Yaki. Jenis penelitian ini ialah eksperimen laboratorium menggunakan Rancangan Acak Lengkap (RAL), dengan 5 macam perlakuan masing-masing perlakuan dengan 3 ulangan. Kelompok 1 sebagai kontrol negatif diberi induksi kalium oksonat), Kelompok 2 sebagai kontrol positif diberi induksi kalium oksonat dan Allopurinol. Kelompok3-5 diberi perlakuan ekstrak buah pinang yaki dengan dosis yang berbeda-beda yaitu 3,6 mg; 7,2 mg dan 14,4 mg. Berdasarkan hasil uji statistik Anova didapatkan kadar asam urat serum berbeda secara bermakna dengan p = 0,002 (<0,05). Hasil dari ekstrak etanol buah pinang yaki di dosis 3,6mg/200grBB, 7,2mg/200grBB dan 14,4mg/200grBB dapat menurunkan kadar asam urat serum dengan uji statistik Anova dengan tingkat kepercayaan 95% Kata kunci : Buah Pinang Yaki, Antihiperurisemia, Anova

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Effect of Apium Graveolens (Celery) Seed Extract on Serum Uric Acid Level of Hyperuricemic Rats and its Comparison with Allopurinol

Journal of Shalamar Medical & Dental College - JSHMDC ◽

10.53685/jshmdc.v2i2.5 ◽

2021 ◽

Vol 2 (2) ◽

pp. 85-91

Author(s):

Abdul Karim ◽

Shabbir Ali bhatti ◽

Mahreen Akhtar ◽

Zartasha Safdar ◽

Noman Johnson ◽

...

Keyword(s):

Uric Acid ◽

Serum Uric Acid ◽

Positive Control ◽

Negative Control ◽

Seed Extract ◽

Control Group ◽

Lowering Effect ◽

Celery Seed ◽

Group B ◽

Potassium Oxonate

Background: Plant derived medicines are widely used in traditional culture all over the world. Objectives: To determine the effect of Celery Seed Extract (CSE) on uric acid levels in hyperuricemic rats and to compare the effect of allopurinol and CSE. Methods: It was an animal experimental research study. Group A served as negative control whereas Group B served as positive control. CSE was given orally to three groups of rats (C, D, and E). One hour prior to administration of CSE; potassium oxonate was injected intraperitoneally in all groups except negative control to induce hyperuricemia. Similarly, group F was given allopurinol one hour after injection of potassium oxonate. Blood samples were collected for uric acid estimation. Results: It was found that administration of both CSE (group C, D, E) and allopurinol (group F) significantly lowered serum uric acid levels (p<0.001) as compared to positive control (group B). Serum uric acid lowering effect of both drugs CSE and allopurinol was found to be statistically significant on day 3rd and day 7th and was almost comparable. Conclusions: Celery seed extract significantly reduces serum uric acid levels in potassium oxonate-induced hyperuricemic rats and its uric acid lowering effect was comparable with that of allopurinol.

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Efektivitas Ekstrak Etanol Daun Durian (Durio zibethinus (Linn.)) sebagai Antihiperurisemia

Jurnal Kefarmasian Indonesia ◽

10.22435/jki.v10i2.2148 ◽

2020 ◽

pp. 130-139

Author(s):

Rela Sonia ◽

Yusnelti Yusnelti ◽

Fitrianingsih Fitrianingsih

Keyword(s):

Uric Acid ◽

Percentage Reduction ◽

Durio Zibethinus ◽

Randomized Design ◽

Oxidase Enzyme ◽

Completely Randomized Design ◽

Ethanol Extracts ◽

Post Hoc ◽

One Way Anova ◽

Potassium Oxonate

Durian (Durio zibethinus Linn.) leaves are known to inhibit the xanthine oxidase enzyme which can reduce uric acid levels in the blood. The purpose of study is to determine the effectiveness of ethanol extracts of durian leaves in reducing uric acid levels in hyperuricemic mice. The mice were induced with chicken’s liver juice 0,2 mL/20 gBW and potassium oxonate 250 mg/kgBW. The animals were mice with uric acid levels above 3,3 mg/dL or 6,2-7,1 mg/dL. The study was used a completely randomized design (CRD) with six treatments and three replications, with three mice in each replications. The treatments group were K+: Allopurinol 10 mg/kgBW; K-: CMC-Na 0.5%; extracts in group P1,P2, P3, P4 are 50; 100; 200; 250 mg/kgBW. The measurements of uric acid levels were performed on day-0, day-6 after induction, and day-15 after administering each group. The data obtained were analyzed by One Way Anova test followed by Duncan's Post Hoc further test. The results showed that durian leaves could reduce uric acid levels in mice blood with the highest percentage reduction was in P2 (100 mg/kgBW) group of 40,53%, followed by P1 (50 mg/kgBW) of 38,68%. The lowest percentage was in the P4 group (250 mg/kgBW) of 22,36%, followed by the P3 group (200 mg/kgBW) of 27,71% and the ED50 value was 61,65 mg/kgBW. In conclusion, ethanol extracts of durian leaves has a significant effect on decreasing total uric acid levels in mice and has potential as an antihyperuricemia agent.

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PENGHAMBATAN KSANTIN OKSIDASE OLEH KOMBINASI EKSTRAK TEMPUYUNG (Sonchus Arvensis) DAN SALAM (Syzygium Polyanthum) PADA MENCIT HIPERURISEMIA

Biomedika ◽

10.23917/biomedika.v4i1.259 ◽

2012 ◽

Vol 5 (1) ◽

Author(s):

Dr. Muhtadi . ◽

Idi Retnani ◽

Nurcahyanti Wahyuningtyas

Keyword(s):

Uric Acid ◽

Xanthine Oxidase ◽

Oxidase Activity ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Xanthine Oxidase Activity ◽

Group I ◽

Sonchus Arvensis ◽

Potassium Oxonate

Tempuyung (Sonchus arvensis) and salam (Syzygium polyanthum) leaves can lower uric acid levels were studied. The purpose of this research was to determine mechanism of combined o f tempuyung and salam extracts to reduce uric acid levels by inhibiting xanthine oxidase in hyperuricemic mice. This research used completely randomized design using mice. Fifteen mice were made hiperuricemic by 250 mg/kgBW potassium oxonate p.o then the mice were divided into 3 groups. Group I induced 10 mg/kgBW allopurinol p.o as positive control, group II induced tempuyung-salam p.o each 100 mg/kgBW as the treatment group, group th III induced 0.5 mL/20 gBW distilled water p.o as negative control. Liver was taken 4 day after 2 hours induced potassium oxonate. Data inhibition xanthine oxidase activity was tested with Mann-Whitney. The result showed that the combination of tempuyung-salam extract each 100 mg/kgBW could inhibit xanthine oxidase activity of 51,16%±2,76%, while allopurinol 90,20%±0,16% (p = 0.000).Keyword: Hyperuricemia, Xanthine Oxidase, Sonchus arvensis, Syzygium polyanthum

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Aktivitas Antihiperurisemia Ekstrak Etanol Daun Sungkai (Peronema canescens Jack) Pada Mencit Putih Jantan

Pharmacon Jurnal Farmasi Indonesia ◽

10.23917/pharmacon.v18i01.12880 ◽

2021 ◽

Vol 18 (1) ◽

pp. 23-37

Author(s):

Madyawati Latief ◽

Indra Lasmana Tarigan ◽

Putri Maya Sari ◽

Fiolita Etsa Aurora

Keyword(s):

Uric Acid ◽

Point Of Care ◽

Ethanol Extract ◽

Positive Control ◽

Negative Control ◽

Test Method ◽

Point Of Care Test ◽

Randomized Design ◽

Wistar Strain ◽

Percent Decline

Hyperuricemia is a disease caused by an increase in uric acid levels in the blood. Currently, various treatments are developed by utilizing natural ingredients as an anti-hyperuricemia. Sungkai (Peronema canescens Jack) is one of the plants that contain a natural compound that can use as an anti-hyperuricemia treatment is the leaves of sungkai (Peronema canescens Jack.). The aim of this study to determine the anti-hyperuricemia activity of the ethanol extract of Sungkai leaves. The test animals used in this study were male white mice Wistar strain. This study used a completely randomized design (CRD) with 5 groups consisting of negative control (Na CMC 0.5%) positive control (allopurinol 10 mg / kg BW), treatment 1 (extract 125 mg / kg BW), treatment 2 (extract 250 mg / kg BW) and treatment 3 (extract 500 mg / kg BW). The parameters seen were uric acid levels measured using the POCT (Point of Care Test) method and analyzed using One Way Anova statistical analysis and Duncan's continued test. Our results showed that the treatment group with a dose of 125-500 mg / Kg BW reduce uric acid levels in mice. The best dose was a dose of 500 mg / Kg BW in reducing uric acid levels in mice with percent decline of 38.66%.

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18. POTENSI EKSTRAK AIR DAUN SERNAI (Wedelia biflora) SEBAGAI ANTINYERI PADA MENCIT (Mus musculus) The Potency of Wedelia biflora Leaf Water Extract as Pain killer on Mice (Mus musculus)

Jurnal Medika Veterinaria ◽

10.21157/j.med.vet..v10i2.4625 ◽

2016 ◽

Vol 8 (1) ◽

Author(s):

Abdul Hayi Amarta Ali

Keyword(s):

Mus Musculus ◽

Water Extract ◽

Positive Control ◽

Negative Control ◽

Hot Plate ◽

Plate Method ◽

Treatment Groups ◽

Randomized Design ◽

Group 2 ◽

Analgesic Potency

The purpose of this research was to find out the potency of Wedelia biflora water extract as pain killer. Completely randomized design (CDR) was used in this study. A total of 30 mice (Mus musculus) were divided into five treatment groups. Mice in group 1 (P0) were administered with mefenamat (positive control) and mice group 2 (P1) were only given aquadest (negative control). Mice in another3 groups (P2, P3, and P4) were administered with Wedelia biflora water extract with the concentration of 100, 75, and 50%, respectively. The painful response time was done using hot plate method and observed at 0, 30, 60, and 90 minutes, whereas abdominal writhing method was applied to count the total of writhing. Data were analyzed using ANOVA followed by Duncan. Result showed that Wedelia biflora water extract with various levels of concentration were affect significantly (P0.05) compared to negative control but no significant different (P0.05) with positive control. The analgesic potency showed significant effect (P0.05) at concentration of 100 and 75% compared to negative control, but no significant different (P0.05) compare to positive control on both hot plate methods and abdominal writhing method, whereas 50% concentration showed analgesic potency (P0.05) only on hot plate method. In conclusion, Wedelia biflora water extract is potential as analgesic on mouse and shows no significant different with mefenamat. ____________________________________________________________________________________________________________________ Key words: analgesik, Wedelia biflora, painful, total of writhe

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Pengaruh Pemberian Kombinasi Ekstrak Etanol Daun Sidaguri (Sida rhombifolia L.) dan Rimpang Jahe Merah (Zingiber officinale Rosc.) pada Mencit Jantan Hiperurisemia

Pustaka Kesehatan ◽

10.19184/pk.v6i2.7566 ◽

2018 ◽

Vol 6 (2) ◽

pp. 205

Author(s):

Kinanthi Putri Rizki ◽

Siti Muslichah ◽

Indah Yulia Ningsih

Keyword(s):

Uric Acid ◽

Zingiber Officinale ◽

Positive Control ◽

Negative Control ◽

Male Mice ◽

Treatment Groups ◽

Ginger Rhizome ◽

Red Ginger ◽

Sida Rhombifolia ◽

Potassium Oxonate

This study aimed to determine the effect of the combination of sidaguri leaves and red ginger rhizome extracts in uric acid levels of hyperuricemic male mice. Thirty-six mice were divided into nine groups including normal group, the positive control (+), negative control (-), extracts of four treatment groups with a variety of combinations and two groups of single extract. Hyperuricemia induction was performed by administering mixture melinjo 10% of the standard feed mice for 7 days dan potassium oxonate 250 mg/kg BB intraperitoneally at 2 hours before blood sampling. The results showed that the combination dose of sidaguri leaves of 50 mg/kg BB and red ginger rhizome 400 mg/kg BB had higher antihyperuricemia activity than other combinations in lowering uric acid levels of mice. Compounds may have antihyperuricemic activity were flavonoids, alkaloids, saponins, tannins, and terpenoids. Keywords: sidaguri leaves, red ginger rhizome, antihyperuricemic activity, uric acid

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UJI AKTIVITAS ANTIHIPERURISEMIA EKSTRAK ETANOL DAUN GEDI HIJAU (Abelmoschus manihot (L.) Medik) PADA TIKUS PUTIH JANTAN GALUR WISTAR (Rattus norvegicus)

PHARMACON ◽

10.35799/pha.8.2019.29365 ◽

2019 ◽

Vol 8 (4) ◽

pp. 878

Author(s):

Defritsevani Y. Umboh ◽

Edwin De Queljoe ◽

Paulina V. Y. Yamlean

Keyword(s):

Uric Acid ◽

Statistical Tests ◽

Ethanol Extract ◽

Positive Control ◽

Negative Control ◽

Group Iii ◽

Group I ◽

Significant Difference ◽

Potential Activity ◽

Ethanol Extracts

ABSTRACTIn the southeast Asian Region showing prevalence for the incidence of hyperuricemia, Indonesia ranks second with 18%. Diseases resulting from hyperuricemia (Excess uric acid) are known as gout. The use of excessive or uric acid lowering drugs such as Allopurinol can cause side effects. Flavonoids are known to inhibit xanthine oxidase, so flavonoids have the potential to be used as antyhiperuricemia agents. Green gedi leaves that have been extracted with 96% ethanol have a total flavonoid content of 41.56%. This study aims to determine the potential activity of reducing uric acid levels from green gedi leaves. This type of research is an experimental study carried out in a laboratory with rat test animals with 5 kind of treatments with 3 treatments per repetition. Hyperuricemia conditioning is done by inducing potassium oxonate intraperitoneally. Group I as negative control was given 1% CMC, group II as positive control was given Allopurinol, group III-V was treated with ethanol extract of green gedi leaves with defferent doses of 3.6mg, 7.2mg and 14.4mg. Based on the ANOVA test results obtained there is a significantly different levels of uric acid with p = 0.038 (<0.05) where the results of decreased of uric acid levels with the ethanol extracts of green gedi have a significant difference (p<0.05) with ANOVA statistical tests with level of confidence 95%. Keywords : Anova, antyhiperuricemiaa, green gedy leaves. ABSTRAKDi Kawasan Asia Tenggara menunjukan prevelensi untuk kejadian hiperurisemia, Indonesia menduduki peringkat kedua dengan angka 18%. Penyakit akibat hiperurisemia (kelebihan asam urat) dikenal sebagai gout atau pirai. Penggunaan obat penurun asam urat seperti Allopurinol yang berlebihan atau terlalu sering dapat menimbulkan efek samping. Flavonoid diketahui dapat menghambat xantin oksidase, sehingga flavonoid berpotensi untuk dijadikan sebagai agen antihiperurisemia. Daun gedi hijau yang telah diekstraksi dengan etanol 96% memiliki total kandungan flavonoid sebesar 41,56%. Penelitian ini bertujuan untuk mengetahui potensi aktivitas penurunan kadar asam urat dari daun gedi hijau. Jenis penelitian ini merupakan penelitian eksperimental yang dilakukan didalam laboratorium dengan hewan uji tikus dengan 5 macam perlakuan setiap perlakuan dengan 3 kali pengulanan. Pengkondisian hiperurisemia dilakukan dengan menginduksi kalium oksonat secara intreperitoneal. Kelompok I sebagai kontrol negatif diberi CMC 1%, kelompok II sebagai kontrol positif diberi Allopurinol, kelompok III- V diberi perlakuan ekstrak etanol daun gedi hijau dengan dosis yang berbeda-beda yaitu 3,6mg, 7,2mg, dan 14,4mg. Berdasarkan hasil uji ANOVA didapatkan kadar asam urat berbeda secara bermakna dengan p = 0,038 (<0,05) dimana hasil penurunan kadar asam urat ekstrak etatnol daun gedi hijau terdapat perbedaan yang signifikan (p<0,05) dengan uji statistik ANOVA dengan tingkat kepercayaan 95%. Kata kunci : Daun gedi hijau, antihiperurisemia, Anova

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UJI AKTIVITAS ANTIHIPERURISEMIA EKSTRAK ETANOL TUMBUHAN SURUHAN (Peperomia pellucida L.Kunth) TERHADAP TIKUS PUTIH JANTAN (Gallur wistar) YANG DI INDUKSI KAFEIN

PHARMACON ◽

10.35799/pha.8.2019.29375 ◽

2019 ◽

Vol 8 (4) ◽

pp. 953

Author(s):

Agista C. Imbar ◽

Edwin De Queljoe ◽

Henki Rotinsulu

Keyword(s):

Uric Acid ◽

Ethanolic Extract ◽

Positive Control ◽

Negative Control ◽

White Rats ◽

Wistar Strain ◽

Ethanol Extracts ◽

Levene Test ◽

Dose Variation ◽

Smirnov Test

ABSTRACTPepper elder plant (Peperomia pellucida L.Kunth) is one of the plants that has the potential to cure various diseases, one of which is to reduce blood uric acid levels. The order contains chemical compounds namely flavonoids and saponins whose mechanism of action inhibits the activity of xantin oxsidase. The aim of this study was to determine the antihyperurismic activity of ethanol extracts of pepper elder plants (Peperomia pellucida L.Kunth) aginst male white rats wistar strain induced with caffeine. This research is experimental. Fifteen rats were divided into 5 treatment groups; each group consisted of 3 rats. The first group was the negative control (K-) given CMC suspension, the second group was positive control (K+) given allopurinol, the three groups were 50mg dose variation groups, the four groups were 100 mg dose variation groups, and the five groups were variations a dose 200 mg. The rats were examined their fasting uric acid levels, then rats were induced by caffeine at dose 300 mg/kgBW. Orally. On the 6th day the uric acid level was examined and then treated according to the group for 9 days, the measurement of uric acid levels was done 3 days. The data obtained were then analyzed statistic using SPSS. Includes test (Levene), test (One sample kolmogrove smirnov-test), ANNOVA, kruskall wallis test. The results showed that the ethanolic extract of the pepper elder plant (Peperomia pellucida L.Kunth) could have a great effect on reducing hyperuricemia at a dose 200mg. Keywords: Antihyperuricemia, Peperomia pellucida L.Kunth.ABSTRAKSuruhan (Peperomia pellucida L.Kunth) merupakan salah satu tanaman yang berpotensi untuk menyembuhkan berbagai penyakit, salah satunya adalah dapat menurunkan kadar asam urat darah. Suruhan mengandung senyawa kimia yaitu flavonoid dan saponin yang mekanisme kerjanya menghambat aktivitas Xantin Oksidase. Penelitian ini bertujuan untuk mengetahui aktivitas Antihiperurisemia ekstrak etanol tumbuhan suruhan (Peperomia pellucida L.Kunth) terhadap tikus putih jantan galur wistar yang di induksi kafein. Penelitian ini bersifat eksperimental. 15 ekor tikus dibagi ke dalam 5 kelompok perlakuan masing- masing kelompok terdiri dari 3 ekor tikus. Kelompok pertama kelompok Kontrol negatif (K-) yang diberikan suspensi CMC, Kelompok kedua kontrol positif (K+) yang diberikan allopurinol, kelompok tiga kelompok variasi dosis 50 mg, kelompok empat kelompok variasi dosis 100 mg, dan kelompok lima kelompok variasi dosis 200 mg. Sebelumnya tikus dilakukan pemeriksaan kadar asam urat puasa, selanjutnya tikus diinduksi kafein dosis 300 mg. secara oral. Pada hari ke 6 diperiksa kadar asam urat kemudian dilakukan perlakuan sesuai kelompok selama 9 hari, pengukuran kadar asam urat dilakukan 3 hari sekali. Data yang diperoleh kemudian dianalisis secara statistik menggunakan SPSS. Meliputi uji (Levene), uji (One-Sample kolmogorove Smirnov-test), ANNOVA, uji kruskall wallis. Hasil penelitian menunjukan bahwa ekstrak etanol tumbuhan Suruhan (Peperomia pellucida L.Kunth) dapat memberikan efek penurunan Hiperurisemia yang besar pada dosis 200 mgKata kunci: Antihiperurisemia, Peperomia pellucida L.Kunth.

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Antihyperuricemia Activity of the Ethyl Acetate Fraction of Bilimbi Leaves (Averrhoa bilimbi L.)

Jurnal Farmasi Udayana ◽

10.24843/jfu.2021.v10.i02.p10 ◽

2021 ◽

pp. 168

Author(s):

Fitra Fauziah ◽

Ifora Ifora ◽

Silvi Karlila Sari

Keyword(s):

Uric Acid ◽

Ethyl Acetate ◽

Biological Marker ◽

Ethyl Acetate Fraction ◽

Positive Control ◽

Negative Control ◽

The Body ◽

Flavonoid Compounds ◽

Traditional Medicinal Plants ◽

Potassium Oxonate

Hyperuricemia is a condition where there is an increase in uric acid levels above normal. An increase in the number of leukocytes is also a biological marker of hyperuricemia. The leaves of bilimbi (Averrhoa bilimbi L.) are one of the traditional medicinal plants which contain alkaloids, tannins, steroids, and flavonoid compounds. Flavonoid compounds are thought to inhibit the formation of uric acid in the body. This study investigates the antihyperuricemia activity of the ethyl acetate fraction of bilimbi leaves. The animals were male white mice grouped into six groups: the normal group, negative control, positive control (allopurinol), and the group was given the ethyl acetate fraction of bilimbi leaves at doses of 100, 200, and 400 mg/kg BW. Hyperuricemia induction is by giving high-purine diets and potassium oxonate. The results showed that the variation of the ethyl acetate fraction of bilimbi leaves significantly reduce uric acid levels and the number of leukocyte cells (sig <0.05). Giving ethyl acetate fraction at a dose of 400 mg/kg BW showed the best reduction in uric acid and leukocyte levels.

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Hydrogel Activity Test from Kirinyu Leaf Ethanol Extract (Chromolaenaodorata (L.) R. King & H. Rob) and Combination of Kirinyu Leaf Ethanol Extract with Collagen As A Healing Wound Excision.

Asian Journal of Pharmaceutical Research and Development ◽

10.22270/ajprd.v8i1.651 ◽

2020 ◽

Vol 8 (1) ◽

pp. 35-37

Author(s):

Thursina C S ◽

Julia R ◽

Sumaiyah S

Keyword(s):

Ethanol Extract ◽

Positive Control ◽

Negative Control ◽

Activity Test ◽

Collagen Group ◽

Healing Wound ◽

Wound Excision ◽

Group 2 ◽

Ethanol Extracts ◽

Group 1

Objective: the purpose of this study was to determine the activity of the kirinyu leaf ethanol extract hydrogel preparation and the combination of the kirinyu leaf ethanol extract with collagen as a healing wound excision. Method: This research is experimental, where the ethanol extracts of Kirinyu leaves and collagen are formulated in the form of hydrogel preparations and tested on the healing of excision wounds in rats. Testing wound healing activities were divided into 6 groups. The Group 1 (negative control) were given hydrogel base without Kirinyu leaf ethanol extract in combination with collagen, group 2 (positive control) were given Octenilin®gel, groups 3 and 4, were given hydrogel of Kirinyu leaf ethanol extract while groups 5 and 6 were given hydrogel extract of kirinyu leaves combined with collagen. Result: The results of percent reduction in wound diameter showed that groups 3 and 4 gave significant differences compared to group 1 which began to be seen on the 10th day (p <0.05). While groups 5 and 6 gave significant differences that began to be seen on the 8th day (p <0.05). Conclusion:Hydrogel preparation in group 6 has the most potential in healing excision wounds.

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