scholarly journals Postharvest Treatment of Chinese Kale (Brassica oleracea var. alboglabra) by Pulse Light to Removal of Microbial Load, Pesticide Residue and Integrity of Physicochemical Quality and Phytochemical Constituent

Author(s):  
Nguyen Phuoc Minh

Existence of microorganisms, pesticide residue on fresh vegetables has a potential hazard to human health. The demand for safe green Chinese kale (Brassica oleracea var. alboglabra) has increased recently. Chinese kale is a healthy botanical attached to the Brassicaceae class. It contains numerous nutritional and phytochemical constituents beneficial for human health. Besides health benefits, this green vegetable also poses food safety concerns due to pathogen and pesticide residue during cultivation. Non-thermal physical technology like pulsed light (PL) will be a promising alternative to eradicate microbial and pesticide residue while preserving the best physicochemical properties and phytochemical components. This research evaluated the influence of different pulsed light intensities (1.2-10.8 J/cm2) on the removal of microbial load and pesticide residue as well as weight attrition, texture hardness, dry matter, vitamin C, total phenolic content in the treated Chinese kale. Results showed that pulsed light intensity 8.4 J/cm2 was appropriate to completely eliminate pathogenic bacteria such as Escherichia coli, Staphylococcus aureus, Salmonella; pesticide substances such as carbendazim, abamectin, cypermethrin, chlorpyrifos ethyl, mancozeb. At pulsed light intensity 8.4 J/cm2, weight attrition in the treated sample was lower than weight attrition in the untreated; meanwhile textural hardness, dry matter, ascorbic acid and total phenolic content remained higher in the treated sample compared to the untreated. The results reveals that the pulsed light technique should be applied as a promising decontamination approach for removal of the pathogen as well as pesticide residue with minor impact on physicochemical properties and phytochemical constituents.

Nova Scientia ◽  
2020 ◽  
Vol 12 (24) ◽  
Author(s):  
Rey David Vargas Sánchez ◽  
Evelin Martínez Benavidez ◽  
Javier Hernández ◽  
Gastón Ramón Torrescano Urrutia ◽  
Armida Sánchez Escalante

In this study the effect of pollen source (mesquite and catclaw) on the sensory characteristics (appearance, color, aroma, taste, consistency and visible impurities), and physicochemical properties of raw propolis, and the phenolic content and biological activities of propolis extracts (PEs) was determined. The phenolic composition of PEs was determined by the total phenolic (TPC), flavone and flavonol (FFC), and flavanone and dihydroflavonol content (FDC). The individual phenolic components were analyzed by HPLC-DAD. The antioxidant activity was determined by the ferric-reducing power (FRAP) and free-radical scavenging activity (FRS). The antibacterial activity was evaluated against Gram-positive (Staphylococcus aureus and Listeria innocua) and Gram-negative (Echerichia coli and Salmonella thyphimurium) bacteria. The results showed that sensory characteristic and physicochemical properties of mesquite and catclaw propolis complied with international quality regulations. Fifteen phenolic compounds were identified, of which pinocembrin, naringenin, galangin, chrysin and quercetin were found a high concentration (> 3 mg/g). Mesquite propolis had the highest phenolic content (TFC and FDC), as well as antioxidant activity (> 2.5 mg Fe (II) equivalent/g; > 40% of DPPH radical inhibition) and antibacterial activity against Gram-positive bacterias in the order S. aureus > L. innocua (> 50% of inhibition for both bacterias at 500 µg/mL). These results indicating that pollen source affect the sensory characteristics and physicochemical properties of propolis, as well as the biological activity of their extracts.


2007 ◽  
Vol 2007 ◽  
pp. 223-223 ◽  
Author(s):  
Atiyeh Bohluli ◽  
Abasali Naserian ◽  
Reza Valizadeh ◽  
Fereydon Eftekarshahroodi

Pistachio (pistachio vera) is from Anacardiaceae family. According to the FAO (2003) report, Iran is the largest pistachio producer in the world (more than 310,000 tons). The most three important exported pistachio nuts of Iran are Ohadi, Akbari and Kaleghuchi (Mohammadi, 2005). About 150,000 tons in DM of pistachio by-product (PB) is produced from dehulling process in Iran, annually. This by-product is mainly consisted of pistachio hulls (PH), and then peduncles, leaves and a little amount of mesocarp and kernels. In this experiment the chemical composition, Total Phenolic Content (TPC) and tannin amount of PB and PH of Ohadi, Kaleghuchi and white varieties were determined; also, In Vitro Dry Matter and Organic Matter Digestibility (IVDMD and IVOMD) were measured.


Author(s):  
Rasna Gupta ◽  
Ram Lakhan Singh ◽  
Neeraja Dwivedi

Objective: To investigate the antioxidant activity of ethanolic extract of Terminalia bellerica fruit pulp (TBFP), seed (TBS) and bark (TBB), and identification of phytochemical constituents of said extracts.Methods: The antioxidant potential of T. bellerica (TB) parts was evaluated by free radical scavenging activity (FRSA), superoxide anion radical scavenging activity (SARSA), hydroxyl radical scavenging activity (HRSA) and compared with reference standard quercetin. Lipid peroxidation (LPO), ferric thiocyanate activity (FTC) and reducing power (RP) of the plant extracts were also examined. The phytochemical constituents of said extracts have been quantified by gas chromatography-mass spectrometer (GC-MS) method.Results: Ethanolic extract of TBFP exhibited high phenolic content (254.33) followed by TBS (227.33) and TBB (185) mg/g of GAE. TBFP showed lowest IC50 for FRSA (62 µg/ml), SARSA (39 µg/ml) and HRSA (27 µg/ml) and highest RP (3.39 ascorbic acid equivalents/ml). TBB showed lowest IC50 for LPO (48 µg/ml) whereas TBS showed lowest IC50 for FTC (109 µg/ml). Predominant phyto-compounds present in TB extracts were quinic acid, gallic acid, ethyl galate, 9, 12 octadecadienoic acid and glucopyranose in varying concentrations as analyzed by GC-MS.Conclusion: On the basis of correlation of antioxidant studies with total phenolic content (TPC) and GC-MS analysis of different part of TB, it may be concluded that high concentration of total phenolics and other bioactive phytochemicals of TB extracts make it a potential source of nutraceutical antioxidants.


2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Afrizal Itam ◽  
Mutia Siska Wati ◽  
Vina Agustin ◽  
Nursal Sabri ◽  
Rafika Aris Jumanah ◽  
...  

Syzygium aqueum, consisting of various fruit colors, is one of the plants that have been used as traditional medicine. This study aims to evaluate and compare phytochemical, antioxidant, and cytotoxic activities and total phenolic content of leaves and stem bark extracts of S. aqueum with pink and red fruits, in order to identify the best extract that can be used as a natural antioxidant. Phytochemical constituents were evaluated qualitatively using chemicals, while cytotoxic activities were identified using the brine shrimp lethality test. Total phenolic content was determined via the Folin–Ciocalteu method. Leaves and stem bark of S. aqueum contained flavonoids, phenolics, and triterpenoids, but the stem bark also contained saponins and alkaloids. Methanol and ethyl acetate extracts of leaves and stem bark were categorized as very powerful antioxidants to DPPH (IC50 9.71–38.69 μg/mL) and hydrogen peroxide (IC50 16.44–44.02 μg/mL), while hexane extracts were inactive. Methanol, ethyl acetate, and hexane extracts of leaves and stem bark were categorized as moderately cytotoxic to A. salina larvae (LC50 104.04–440.65 μg/mL). Comparing leaves and stem barks, antioxidant and cytotoxic activities of stem bark extracts were higher than those of leaves extracts. Total phenolic content of leaves extracts was higher than that of stem bark extracts where the order of total phenolic content progressed from methanol extracts > ethyl acetate extracts > hexane extracts. Therefore, the stem bark of S. aqueum was identified as the better source of natural antioxidant compared with the leaves.


2018 ◽  
Vol 47 (4) ◽  
pp. 847-854
Author(s):  
Seyed Mahdi Tabei ◽  
Ardalan Alizadeh

The essential oil of Salvia nemorosa L. was analyzed by GC-MS. The major constituents of the oil were E-caryophyllene (26.37%), phytol (16.92%), germacrene-D (15.34%), sabinene (12.86%), caryophyllene oxide (6.33%), bicyclogermacrene (4.71%) and n-tetradecane (2.45%). The total phenolic content and the antioxidant activity of methanolic extracts were determined with the Folin-Ciocalteau reagent and by the 2,2- diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay, respectively. The antimicrobial activity of the essential oil was individually evaluated against Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli and Candida albicans using disc diffusion and serial dilution methods. The oil showed good antimicrobial activity against four medically important pathogens.


2012 ◽  
Vol 90 (3) ◽  
pp. 433-441 ◽  
Author(s):  
Nadia Djendoubi Mrad ◽  
Nourhène Boudhrioua ◽  
Nabil Kechaou ◽  
Francis Courtois ◽  
Catherine Bonazzi

Author(s):  
Ion Trandafir ◽  
Sina Cosmulescu ◽  
Violeta Nour

Abstract Total phenolic content, total flavonoid content, antioxidant activity and individual phenolic compounds were assessed in full fat and defatted walnut kernel. For quantification of phenolic fraction of walnut kernels, two different solvents (methanol and ethanol) and two methods of extraction (ultrasonic-assisted extraction and Soxhlet extraction) were tested. Total phenolics, flavonoid content and antioxidant capacity of alcoholic extracts varied depending on the solvent used and extraction methods. Seventeen phenolic compounds were detected and the study provides evidence on high phenolic contents and high antioxidant potential of full fat walnut kernel and defatted walnut kernel. The Soxhlet extraction is the best in terms of the amounts of total phenolic content (2,089.2 mg gallic acid equivalent/100 g dry matter), while the ultrasonic assisted extraction is a fast method but resulted in significantly lower phenolic content (667.3–1,426.8 mg gallic acid equivalent /100 g dry matter). The concentrations of phenolics (especially (+)-catechin hydrate, juglone, vanillic acid, caffeic acid, ferulic acid, sinapic acid, salicylic acid and ellagic acid) are many fold lower in ultrasonic-assisted extraction as compare to the Soxhlet method using the same extraction solvent. The results of this study provide evidence on high phenolic contents and high antioxidant potential of full fat and defatted walnut kernel.


2018 ◽  
Vol 24 (2) ◽  
pp. 93-100
Author(s):  
Le Quoc ◽  
Nguyen Muoi

Polyphenol is a valuable compound found in plants. Unfortunately, it is quite sensitive to heat, light and oxygen in the air. This is a disadvantage making the storage of these compounds for longer periods of time difficult. However, this problem can be overcome by encapsulation with carrier agents as maltodextrin, gum arabic, modified starch, etc. The efficiency of maltodextrin (MD, DE16-19) and gum arabic (GA) on spray drying of Polygonum multiflorum Thunb. root extract was investigated. The incorporation of gum arabic to the extract had the total polyphenol content (TPC) and antioxidant capacity (AC) higher than maltodextrin. The obtained powders from gum arabic and maltodextrin were analyzed for encapsulation yield, moisture content, color parameters, total phenolic content, antioxidant capacity, bulk density, wettability, hygroscopicity, water solubility index, particle size and microstructure. The results showed the types of carrier agents which significantly affected the physicochemical properties of powders produced by spray drying.


2021 ◽  
Vol 33 (2) ◽  
pp. 399-403
Author(s):  
G. Vinayak ◽  
N. Praveen

In present study, the samples collected from different locations of Southern India viz., Yellapur Beltargadde, Siddapur, Joida, Ankola, Sirsi Kangod, Yellapur Shalabail, Sirsi Bairumbe, Karwar of Karnataka and Kasaragod from Kerala were analyzed for the andrographolide content, total phenolic and flavonoid content and screened for their antioxidant potential. The A. paniculata leaves were extracted with three different solvents (chloroform, methanol and water) and methanolic extract of Siddapur showed highest (8.82 ± 0.25 mg/g DW) amount of phenolic content whereas, aqueous extract of Ankola (3.00 ± 1.18 mg/g DW) showed the least amount. Chloroform extract of Yellapur Beltargadde village showed highest quantity i.e. 1.87 ± 0.50 mg/g DW of flavonoid content and aqueous extract of Yellapur Beltargadde showed 0.30 ± 0.20 mg/g DW which was least among all the tested samples. The sample collected from Karwar was found to have highest andrographolide content (9.36 ± 0.02 mg/g DW) followed by Yellapur Beltargadde sample with 7.29 ± 0.01 mg/g DW and Sirsi Kasaragod has the lowest contents of 1.54 ± 0.1 mg/g DW when analyzed through HPLC. Among the nine locations, methanol extract from Joida showed highest percentage of scavenging activity (91.95%) followed by methanol extract of Ankola (90.42%) and chloroform extract of Siddapur (77.31%) which was the lowest value of all samples tested.


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