scholarly journals Formulation of Anti Acne Sheet Mask from Bandotan Leaf Extract (Ageratum conyzoides L.) against Propionibacterium acnes

2021 ◽  
Vol 11 (6-S) ◽  
pp. 123-127
Author(s):  
Pricella Ginting ◽  
Leny Leny ◽  
Ihsanul Hafiz ◽  
Romauli Hasibuan
Keyword(s):  
Leaf Extract ◽  
Propionibacterium Acnes ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Ageratum Conyzoides ◽  
Mueller Hinton ◽  
Mueller Hinton Agar ◽  
Inhibitory Power ◽  
The One

Acne can occur due to increased sebum excretion, inflammation of the skin triggered by the bacteria Propionibacterium acnes, Staphylococcus epidermidis, Staphylococcus aureus. The purpose of this study was to determine the formulation of sheet mask preparations of bandotan leaf extract (Ageratum conyzoides L.) in inhibiting the growth of Propionibacterium acnes bacteria. This type of research is an experimental study which include plant identification, making ethanol extract of bandotan leaves, making sheet mask formulations, evaluating the characteristics of the preparation and testing antibacterial activity using disc paper method using Mueller Hinton Agar media. The extract was carried out by maceration using 70% ethanol as a solvent. Testing the effectiveness of antibacterial by measuring the diameter of the inhibition zone, after that the data were analyzed using the One Way ANOVA statistical test. The results of the evaluation of the preparation showed that the preparation was homogenous, with pH ranging from 4.5 to 6.5 and the preparation did not cause irritation on volunteers skin. The results of the inhibition zone measurements showed that the inhibition zones at 2.5% concentration is 4.7mm, 5% (6.83mm), 7.5% (10.2mm) and positive control (20.57mm). This means that the higher the concentration, the larger the diameter of the inhibition zone obtained. The conclusion in this study is bandotan leaf extract (Ageratum conyzoides L.) can be formulated into anti acne sheet mask which is stable during storage with a strong inhibitory power at 7.5% concentration, which is 10.2 mm. Keywords: Ageratum conyzoides L., bandotan leaf, Propionibacterium acnes, sheet mask

scholarly journals EFFECTIVENESS OF ANTIBACTERIAL EXTRACT OF KENOP (GOMPHRENA GLOBOSA) FLOWER EXTRACT AGAINST GROWTH OF PROPIONIBACTERIUM ACNES BACTERIA

2020 ◽  
Vol 4 (2) ◽  
pp. 115
Author(s):  
Elvina Veronica ◽  
Sang Ayu Arta Suyantari ◽  
Widia Danis Swari ◽  
Ni Made Ari Purwaningrum ◽  
Agung bagus sista Satyarsa ◽  
...  
Keyword(s):  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Treatment Groups ◽  
Gomphrena Globosa ◽  
Mueller Hinton ◽  
Mueller Hinton Agar ◽  
Herbal Plants ◽  
Agar Media ◽  
Inhibitory Power

Acne is a chronic inflammation of the pilosebaceous tissue caused by the bacterium Propionibacterium acne (P.acnes) which often experiences antibiotic resistance. Research on the benefits of herbal plants in dealing with acne is still needed, one of which is the use of Knobs flowers (Gomphrena globosa) which is easily found and has potential as an antioxidant and antibacterial. This study aims to determine the effectiveness of the extract of the flower knob in inhibiting the growth of P.acnes bacteria. The design of this study was purely experimental with the Kirby-Bauer method using MHA (Mueller Hinton Agar) media. A positive control using clindamycin antibiotics and negative control using aquadest. The treatment group consisted of 25%, 50%, 75%, and 100% knob methanol extract with 3 replications. Data analysis using One Way ANOVA to find out the average difference between treatment groups. Based on the results of the study, it was found that the higher the concentration of the extract, the greater the inhibitory power as long as the extract had not reached saturated concentration (p <0.05). There is a decrease in the inhibition zone diameter at 100% concentration because it is influenced by the diffusion rate of a compound and other factors.

scholarly journals Effect of ethanol extract of robusta coffee leaves (Coffea canephora var. robusta) against Streptococcus mutans growth

2021 ◽  
Vol 5 (2) ◽  
pp. 75-79
Author(s):  
Putri Rahmi Noviyandri ◽  
Diotama Nuza ◽  
Suzanna Sungkar
Keyword(s):  
Leaf Extract ◽  
Antibacterial Effect ◽  
Ethanol Extract ◽  
Coffea Canephora ◽  
A Value ◽  
Mueller Hinton ◽  
Significant Difference ◽  
Mueller Hinton Agar ◽  
The One ◽  
Inhibition Potency

The study to determine the inhibition capability of robusta coffee's (Coffea canephora var. robusta) leaf extract against S. mutans growth. Robusta coffee's (Coffea canephora var. robusta) leaf extract was made by maceration method using 96% ethanol. The antibacterial examination using well diffusion on Mueller Hinton Agar (MHA) media. The concentration of robusta coffee's (Coffea canephora var. robusta) leaf extract used in this study was 25%, 50%, and 75%. The study was analyzed using the One Way Analysis of Variance (ANOVA) test and continued with the Least Significant Difference (LSD) test. The results showed a value of p0.05, which proves that robusta coffee's (Coffea canephora var. robusta) leaf extract has an antibacterial effect on the growth of S. mutans with low inhibition potency

scholarly journals Uji Daya Hambat Ekstrak Etanol Batang Saluang Belum (Luvunga sarmentosa Kurz) Terhadap Bakteri Propionibacterium acnes

2021 ◽  
Vol 8 (1) ◽  
pp. 65
Author(s):  
Rezqi Handayani ◽  
Nurul Qamariah ◽  
Muhammad Izmiansyah
Keyword(s):  
Secondary Metabolites ◽  
Inhibitory Activity ◽  
Propionibacterium Acnes ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Inhibition Test ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Percolation Method

Saluang Belum (Luvunga sarmentosa Kurz) adalah tumbuhan yang dikenal sebagai obat tradisional di Kalimantan Tengah. Batang L. sarmentosa Kurz dipercayai memiliki manfaat secara empiris sebagai antioksidan yang dimanfaatkan sebagai anti-aging (anti penuaan dini). Batang L. sarmentosa Kurz mengandung metabolit sekunder yaitu tanin, saponin, steroid dan flavonoid yang diduga memiliki aktivitas sebagai antibakteri. Berdasarkan khasiat empiris dan kandungan metabolit sekunder yang ada dalam tumbuhan L. sarmentosa Kurz, maka dilakukan penelitian tentang aktivitas daya hambat ekstrak etanol tumbuhan ini terhadap bakteri P. acnes yang merupakan salah satu bakteri penyebab jerawat. Tujuan dari penelitian ini adalah untuk mengetahui aktivitas daya hambat ekstrak etanol batang L. sarmentosa Kurz terhadap bakteri P.acnes. Metode penelitian yang dilakukan pada penelitian ini dimulai dari pengambilan simplisia, pembuatan ekstrak etanol dengan metode perkolasi dan uji daya hambat dengan menggunakan metode Disk Difusion yang menggunakan kontrol positif antibiotik klindamicin. Analisis data dilakukan dengan membandingkan lebar zona hambat dari ekstrak etanol batang L. sarmentosa Kurz  dengan kategori penghambatan antimikroba klindamisin berdasarkan diameter zona hambat dari Clinical and Laboratory Standards Institute.  Hasil uji daya hambat didapatkan zona hambat ekstrak etanol batang Saluang Belum pada semua konsentrasi 0,5%, 1%, 5%, 10% dan 15% secara berturut-turut yaitu  Intermediate, Resistant, Intermediate, Susceptible dan Susceptible dan hasil dari zona hambat klindamicin pada semua konsentrasi 0,5%,1%,5%,10%,dan 15% adalah susceptible. Simpulan dari penelitian ini adalah ekstrak etanol batang L. sarmentosa Kurz mempunyai kemampuan dalam menghambat pertumbuhan bakteri P.acnes.           Kata Kunci: Obat Tradisional, Jerawat, Uji Daya Hambat, Propionibacterium acnes, Batang Saluang Belum Saluang belum (Luvunga sarmentosa Kurz) is one of traditional medicine in Central Kalimantan. The stem of L. sarmentosa Kurz is believed to have empirical benefits as an antioxidant which is used as anti-ageing (anti-ageing). The stem of L. sarmentosa Kurz contains secondary metabolites, namely tannins, saponins, steroids and flavonoids which are thought to have antibacterial activity. Based on the empirical properties and the content of secondary metabolites in L. sarmentosa Kurz, a study was conducted on the inhibitory activity of this plant's ethanol extract against P. acnes, which is one of the bacteria that causes acne. The purpose of this study was to determine the inhibitory activity of the ethanol extract of L. sarmentosa Kurz stem against P.acnes bacteria. The research method carried out in this study started from taking simplicia, making ethanol extract using the percolation method and the inhibition test using the disk diffusion method which used a positive control of the clindamycin antibiotic. Data analysis was performed by comparing the inhibition zone width of the ethanol extract of L. sarmentosa Kurz stem with the clindamycin antimicrobial inhibition category based on the inhibition zone diameter of the Clinical and Laboratory Standards Institute. The results of the inhibition test obtained the inhibition zone of Saluang Belum stem ethanol extract at all concentrations of 0.5%, 1%, 5%, 10% and 15% respectively, Intermediate, Resistant, Intermediate, Susceptible and Susceptible and the results of the inhibition zone. Clindamycin at all concentrations of 0.5%, 1%, 5%, 10%, and 15% are susceptible. This research concludes that the ethanol extract of L. sarmentosa Kurz stem can inhibit the growth of P.acnes bacteria.

scholarly journals The Inhibitory Power Extract Of Balsamica Plant (Impatiens balsamica L) on Aeromonas hydrophila Bacteria Growth

2014 ◽  
Vol 2 (2) ◽  
pp. 40
Author(s):  
Galih Arif Kusuma ◽  
Sammy N. J. Longdong ◽  
Reiny A. Tumbol
Keyword(s):  
Antibacterial Activity ◽  
Aeromonas Hydrophila ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Bacteria Growth ◽  
Activity Test ◽  
Inhibitory Power

This research aimed to assess the potential use of extracts of leaves, flowers, and stems of the balsamica plant increase inhibitory effect on the growth of Aeromonas hydrophila. The content of the plant contains a compound balsamica naphthoquinone, coumarin derivatives, tannins, flavonoids, and steroids. The active compounds have the ability as an antimicrobial. A hidrophyla bacteria, including opportunistic pathogens are almost always found in water and often cause disease when the fish in adverse conditions. Antibacterial activity test in this research using the spread plate method. Data obtained in the form of the extract, the results of bacterial inoculation, and the inhibition test results, will be analyzed, displayed with pictures and described descriptively. The result of the process of maceration extraction using ethanol 70% was obtained three extracts are concentrated leaf extract: 28.75 g, flower: 12.82 g, and rods: 29.48 g. The result of antibacterial extracts of leaves, stems, and flowers balsamica plant showed inhibitory activity on the A hydrophila bacteria that seems to be indicated by a clear zone around the paper disc. Based on the classification of leaf extract and extract of the stem can be classified in the class of strong, because the inhibition zone formed by the leaf extract is equal to 11.2 mm, and extract stem with 13.7 mm inhibition zone. Ability flower extract in inhibiting the growth of A hidrophila bacteria with 21.4 mm inhibition zone showed that the extract was included in a very strong class. Keywords: The ethanol extract balsamica, Impatiens balsamica L, Aeromonas hydrophila, antibacterial activity test.

scholarly journals The Effect of Various Concentrations of Ethanol Extract of the Leaves of Paederia foetida L. on the Growth of Escherichia Coli Bacteria

2021 ◽  
Vol 11 (6) ◽  
pp. 61-67
Author(s):  
Hertina Silaban
Keyword(s):  
Escherichia Coli ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Rare Plants ◽  
E Coli ◽  
Paederia Foetida ◽  
Escherichia Coli Bacteria ◽  
The One

Bacterial infection of Escherichia coli (E. coli) as the cause of gastrointestinal disorders in humans has increased their prevalence. Treatment using natural ingredients can be a choice of therapy because of the minimal side effects. One of the rare plants believed by the community as an antibacterial is stinking vin’e known as the ‘leaf fart’. The purpose of this research is for knowing the activity of the ethanol extract of Paederia foetida L can affect the growth of E.coli. The serial diffusion disc method is being used as the antibacterial activity test. The concentration  of this extract are 10%, 20%, 40%, 80%, 100% with positive control (ciprofloxacin) and negative control (aqua dest). The inhibition zone diameter characterized the effect of Extract on bacterial growth were 6.16 mm of the concentration 10%, 6.667 mm of the concentration 20%, 7.10 mm of the concentration 40 %, 7.78 mm of the concentration 80%, and 10.03 mm of the concentration 100%. As for the negative control has no effect. The study stated that the higher concentration of antibacterial agent used, the greater the inhibition zone formed. Based on the result of the analysis of the data by using the One-Way ANOVA Test showed a probability value (p) = 0.000 or value (p) < 0.05, that H0 is rejected and H1 is accepted. The conclusion is that the Extract of stinking vin’e has an antibacterial effect on the growth of E.coli. Keywords: Antibacterial, E.coli, Extract of  Sembukan leaf

scholarly journals Uji Daya Hambat Isolat Actinomycetes sebagai Antibakteri terhadap Pertumbuhan Pseudomonas aeruginosa ATCC 27853 secara In Vitro

2021 ◽  
Vol 10 (1) ◽  
pp. 16
Author(s):  
Saskia Arientika Wahyuningrum ◽  
Meiskha Bahar ◽  
Andri Pramesyanti Pramono
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
P Value ◽  
Gram Positive Bacteria ◽  
Mueller Hinton ◽  
Mueller Hinton Agar ◽  
The One ◽  
One Way Anova

Pneumonia is a lung parenchymal infection caused by Pseudomonas aeruginosa.It is Gram negative bacteria that have developed antibiotic resistance. Actinomycetes are Gram-positive bacteria that produce secondary metabolites which have the ability as antimicrobial. Objectives: To identified the ability of Actinomycetes isolates to inhibit the growth of the bacterium Pseudomonas aeruginosa ATCC 27853. The samples in this experiment were from Kebun Raya Bogor that had been rejuvenated on Starch Casein Agar (SCA). Methods: Six dilution series 10-1; 10-2; 10-3; 10-4; 10-5; 10-6 Actinomycetes isolates were used to observe the inhibition zone of P.aeruginosa growth on Mueller Hinton Agar (MHA) media by diffusion method. Results: The effective incubation time occurred at 24 hours, and then it resulted in the average clear zone diameter of 14.70 mm, 10.57 mm, 8.53 mm, 8.47 mm, 6.97 mm, and 5.30 mm. The results of the One – Way Anova test with p-value = 0.000 (p < 0.005) showed some differences at each concentration to inhibit the growth of P.aeruginosa ATCC 27853 at 24 hours incubation period. Conclusion: The most effective concentration of Actinomycetes isolates that can potentially be antibacterial was the concentration of 10-1 with potential solid inhibitory power.Keywords: Actinomycetes, antibacterial, Pseudomonas aeruginosa

scholarly journals Antibacterial Activity of Cold Powder Preparation of (Ethanol Extract) Starfruit Leaf (Averrhoa bilimbi Linn.) Against Propionibacterium acnes

2020 ◽  
Vol 6 (2) ◽  
pp. 120-128
Author(s):  
Helen Anjelina Simanjuntak ◽  
Kasta Gurning ◽  
Verawati Br. Sinaga
Keyword(s):  
Antibacterial Activity ◽  
Medicinal Plant ◽  
Research Method ◽  
Propionibacterium Acnes ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Distilled Water ◽  
Powder Preparation

Acne is a skin disease that occurs due to chronic trade that is triggered by the bacterium Propionibacterium acnes. Starfruit leaf (Averrhoa bilimbi Linn.) as a traditional medicinal plant which has antibacterial activity. The purpose of this study was to determine the antibacterial activity from ethanol extract in the preparations cold powder of starfruit leaf (Averrhoa bilimbi Linn.) againts Propionibacterium acnes. The research method was carried out experimentally, and testing for antibacterial activity was carried out using agar diffusion methods. Cold powder formulation such as F1 (5%), F2 (10%), F3 (15%), F4 (20%), positive control with clindamycin, negative control with distilled water. The results showed that the F3 (15%) and F4 (20%) formulations were more effective in inhibiting the growth of Propionibacterium acnes bacteria with inhibition zone diameters of 13.66 mm and 15.16 mm in the strong category

scholarly journals ANTIBACTERIAL ACTIVITY OF MIANA LEAF ESTRACT (Coleus atropurpereus) ON Steptococcus aureus

2020 ◽  
Vol 15 (1) ◽  
pp. 1
Author(s):  
Anita Anita ◽  
Mujahidah Basarang ◽  
Rahmawati Rahmawati
Keyword(s):  
Antibacterial Activity ◽  
Leaf Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Laboratory Observation ◽  
Extract Concentration ◽  
Observation Method ◽  
The One ◽  
Growth Of Bacteria

                                               ABSTRACTMiana leaf(Coleus atrropurpureus) is the one of biofarmacology plant which using for antibacterial activity.Miana leaf contains flavonoid, tanin, saponin  which can inhibit growth of bacteria. The aims this research were study antibacterial activity of miana leaf extract on Streptococcus aureus.This research used laboratory observation method which used miana leaf extract concentration 250 mg/ml, 125 mg/ml,  62.5 mg/ml, 31,25 mg/ml, and 15,625 mg/ml. This research used tetracylin for positive control and aquadest for negative control which growth 0n 37o C  used difusion agar method. The results showed antibactrila activity  miana leaf  extract with a concentration 250 mg/ml with inhibition zone 0.00 mm, 125 mg/ml with inhibition zone 0,00 mm, 62,5 mg/ml with inhibition zone 0,00 mm, 31,25 mg/ml with inhibition zone 0,00 mm, and 15,62 mg/ml with inhibition zone 0,00 mm. For positive control showed inhibition zone 27,6 mm and negative control with inhibition zone 0,00 mm. Miana leaf extract concentration 250 mg/ml, 125 mg/ml, 62,5 mg/ml, 31,25 mg/ml and 15,62 mg/ml were not effectively inhibit Streptococcus sp.

scholarly journals UJI BIOAKTIVITAS EKSTRAK DAUN BIDARA BIDARA (ZIZIPHUS MAURITIANA LAM) TERHADAP PERTUMBUHAN STAPHYLOCOCCUS AUREUS

2019 ◽  
Vol 4 (1) ◽  
pp. 49-57
Author(s):  
Aan Yulianingsih ◽  
Dzikra Arwie
Keyword(s):  
Staphylococcus Aureus ◽  
Leaf Extract ◽  
Average Diameter ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Ziziphus Mauritiana ◽  
Diffusion Test ◽  
Normal Microflora ◽  
Inhibitory Power

The background of this study is Staphylococcus aureus is an anaerobic facultative bacterium and one of the normal microflora in the mouth. However, if it is influenced by predisposing factors, it will become a pathogen. Bidara leaves have antimicrobial activity against Staphylococcus aureus, because Bidara leaves contain various compounds including alkaloids, flavonoids, steroids, tannins and essential oils. The purpose of this study was to determine how much inhibitory power produced by Bidara leaf extract on the growth of Staphylococcus aureus bacteria. This research method is a laboratory experimental research using the diffusion test (wells) method. The sample of this study was Staphylococcus aureus. Dilution of Bidara leaf extract consists of 5 concentrations including: 20%, 40%, 60%, 80%, and 100%. Chlorampheniocol is used for Positive control while Negative control uses aquadest. Based on observations show that the average diameter of inhibitory zone Bidara leaf extract at a concentration of 20% with a diameter of 10.5 mm (R), at a concentration of 60% and 80% with a diameter of 16 mm and 18 mm (I), at a concentration of 100% with a diameter of 24 mm (S). It can be concluded that the higher the concentration of Bidara leaf extract, the inhibition zone formed will increase. So Bidara leaves are good for use in treating infections caused by Staphylococcus aureus bacteria.

UJI AKTIVITAS ANTIBAKTERI DARI EKSTRAK ETANOL DAUN PERIA LAUT (Colubrina Asiatica L.) TERHADAP BAKTERI STAPHYLOCOCCUS AUREUS DAN ESCHERICHIA COLI

2019 ◽  
Vol 2 (1) ◽  
pp. 14-18
Author(s):  
Firdaus Fahdi ◽  
Harwitavia Harwitavia ◽  
Herviani Sari
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Antibiotic Drugs ◽  
Escherichia Coli Bacteria

The discovery of new antibiotic drugs is getting more and more reactive. The plant of the peria laut leaf is one of the drugs that is often used as a traditional medicine and contains bioactive compounds of polyphenols, flavonoids, and saponins, which can inhibit antibacterial growth. Purpose of this study was to determine the antibacterial activity of ethanol extract of peria laut leaves (Colubrina asiatica L.) against Staphylococcus aureus and Escherichia coli bacteria. Method the experimental, of the sample used was concentrated marine peria laut leaf extract of 25mg/ml, 50mg/ml, 75mg/ml, 100mg/ml, 200mg/ml, 300mg/ml, 400mg/ml, and 500mg/ml, positive control of amoxicillin tablet 500 mg, negative control of dimethylsulfoxide with the method of disc diffusion testing using media Nutrient Agar. Results the showed thet the peria laut leaf extract positively contained bioactive alkaloid compounds, flavonoids, saponins, steroids, and tannins, and had inhibitory effects on Staphylococcus aureus and Escgerichia coli bacteria with various concentrations which had been tested on the average area of the highest inhibition zone of 18,6 mm in Staphylococcus aureus bacteria, and 10,2 mm in Escherichia coli bacteria. Conclusion peria laut leaf extract (Colubrina asiatica L.) has antibacterial activity against Staphylococcus aureus and Escherichia coli bacteria in the most inhibitory zone at a concentration of 500mg/ml with a diameter of 18,6 in Staphylococcus aureus bacteria and 10,2 in Escherichia coli.

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