scholarly journals Subcronic Toxicity Effect of Ethanol Extract of Bitter Melon Fruit (Momordica charantia L.) on Kidney Function and Histopathological Changes in Wistar Rat

2021 ◽  
Vol 11 (6) ◽  
pp. 143-149
Author(s):  
Welly Ratwita
Keyword(s):  
Kidney Function ◽  
Ethanol Extract ◽  
Momordica Charantia ◽  
Organ Weight ◽  
Female Rats ◽  
Toxic Effects ◽  
Male Rats ◽  
Bitter Melon ◽  
Relative Organ Weight ◽  
Melon Fruit

Bitter melon is a fruit that was famous for its bitter taste and contains chemical compounds that are proven to have effects such as antidiabetic, anti-inflammatory, antipyretic, and antioxidant. One of the prerequisites of a plant to be developed into a drug that is by testing the subchronic toxicity. This study goal to determine the presence of toxic effects that are not detected in the acute toxicity test. A total of 40 wistar rats were divided into group control and 3 treatment groups. Each group consisted of 5 male rats and female rats. Group 1 (control) is only given food (pellets) and drinking water. Group 2, 3 and 4 groups were given ethanol extract of bitter melon pulp (Momordica charantia L.) with multilevels doses 250 mg/kgbw, 500 mg/kgbw, and 1000 mg/kgbw. The observations made for 28 days included the number of death animals, relative organ weight (ROW), ureum, creatinine levels and histopatological changes in kidney. There were no deaths in the entire group, decreasing ureum (p=0,022), creatinine levels (p=0,033) in male group. While in female group ureum decrease not significantly (0.878) and creatinine (0.845). There were no significant changes in ROW (p=0,370 and p=0,394), but there were changes in microscopic structure. In conclusion ethanol extract of bitter melon fruit (Momordica charantia L.) at a  of 250 mg/kgbw, 500 mg/kgbw, and 1000 mg/kgbw does not showed any toxic effects on kidney function and relative organ weight. But microscopically, the administration of ethanol extract of bitter melon fruit caused a toxic effect on the kidney tubules in both male and female groups. Keywords: Momordica charantia L., sub chronic toxicity, ureum, creatinine, histopathological change

scholarly journals UJI AKTIVITAS PENURUNAN KADAR ASAM URAT EKSTRAK ETANOL BUAH PARE (Momordica charantia L.) PADA TIKUS PUTIH JANTAN (Rattus norvegicus) YANG DIINDUKSI KAFEIN

PHARMACON ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 479
Author(s):  
Minar N. S. Gultom ◽  
Edwin De Queljoe ◽  
Elly J. Suoth
Keyword(s):  
Uric Acid ◽  
Rattus Norvegicus ◽  
Ethanol Extract ◽  
Positive Control ◽  
Momordica Charantia ◽  
Male Rats ◽  
Flavonoid Compound ◽  
Bitter Melon ◽  
White Rats ◽  
Melon Fruit

ABSTRACTBitter melon fruit (Momordica charantia L.) is one of the plants that contains flavonoid compound supposedly as anti-hyperuricemic activity. This study aims to determine the potential activity of reducing uric acid levels from the ethanol extract of bitter melon fruit (Momordica charantia L.) on white wistar male rats (Rattus norvegicus) induced with caffeine. This study is a laboratory experiment using a complete randomized design. A total of 15 rats were divided into 5 groups : negative control (CMC 1%), positive control (Allopurinol), and treatment group of bitter melon fruit (Momordica charantia L.) extract 0,9 mg, 1,8 mg, and 3,6 mg. Hyperuricemic condition was induced by oral administration of  27 mg/200 gr caffeine for 6 days. The uric acid levels was measured before the rats was induced, 6 days after induced and every 3 days of 9 days of treatment. The data obtained were analyzed by ANOVA (Analisis of Variance) and LSD (Least Significant Different). The results show that the dose group of the ethanol extrack of bitter melon fruit had comparable with positive control of Allopurinol in decrease uric acid levels. The conclusion was the ethanol extract of bitter melon fruit (Momordica charantia L.) had anti-hyperuricemic activity in male white rats (Rattus norvegicus) dose 0,9 mg, 1,8 mg and 3,6 mg. Keywords  : Momordica charantia L., antihiperuricemia, Rattus norvegicus, caffeine.ABSTRAK Buah pare (Momordica charantia L.) adalah salah satu tanaman yang mengandung flavonoid yang diduga sebagai senyawa yang mempunyai aktivitas antihiperurisemia. Penelitian ini bertujuan untuk mengetahui potensi aktivitas penurunan kadar asam urat dari ekstrak etanol buah pare pada tikus putih jantan galur wistar (Rattus norvegicus). Jenis penelitian ini ialah eksperimen laboratorium menggunakan Rancangan Acak Lengkap. Sebanyak 15 ekor tikus dibagi dalam 5 kelompok perlakuan : kontrol negatif (CMC 1%), kontrol positif (Allopurinol), dan kelompok dosis uji yaitu pemberian ekstrak etanol buah pare 0,9 mg/200 gr BB; 1,8 mg/200 gr BB; 3,6 mg/200 gr BB. Induksi hiperurisemia dilakukan dengan kafein dosis 27 mg/200 gr selama 6 hari. Pengukuran kadar asam urat dilakukan sebelum induksi, 6 hari setelah diinduksi dan setiap 3 hari selama 9 hari perlakuan. Data yang diperoleh kemudian dianalisis secara statistik menggunakan ANOVA (Analysis of Variance) dan LSD (Least Significant Different). Hasil penelitian menunjukkan bahwa kelompok dosis ekstrak etanol buah pare memiliki aktivitas yang sebanding dengan kontrol positif allopurinol dalam menurunkan kadar asam urat. Kesimpulannya ekstrak etanol buah pare (Momordica charantia L.) memiliki aktivitas antihiperurisemia pada tikus putih jantan (Rattus norvegicus) pada dosis 0,9 mg/200 gr BB; 1,8 mg/200 gr BB; 3,6 mg/200 gr BB. Kata kunci : Momordica charantia L., antihiperurisemia, Rattus norvegicus, kafein.

scholarly journals Phytochemical Test of Bitter Melon (Momordica charantia L) Herb and its Effect on Rat Blood Sugar Levels

2021 ◽  
Vol 6 (7) ◽  
pp. 153-163
Author(s):  
Ijazati Alfitroh ◽  
Suhatri Suhatri ◽  
Harrizul Rivai
Keyword(s):  
Blood Sugar ◽  
Negative Control ◽  
Momordica Charantia ◽  
Male Rats ◽  
Bitter Gourd ◽  
Bitter Melon ◽  
Phytochemical Content ◽  
Saponin Content ◽  
Sugar Levels ◽  
Melon Fruit

This study aims to determine the class of chemical compounds, phytochemical levels, antihyperglycemic effects, and histopathology of the pancreas of rats treated with bitter gourd (Momordica charantia L) for the treatment of diabetes mellitus. Bitter gourd is made by adding water, then mashed and squeezed. The ingredients were tested for their phytochemical content and antidiabetic power. 15 male rats were divided into 5 groups. Group 1 as a negative control, namely normal rats without treatment, group 2 as a positive control given alloxan, group 3, namely rats given alloxan and bitter melon fruit 0.9 mL, group 4, namely rats given alloxan and bitter melon ingredients 1.8 mL, and group 5, namely rats, were given alloxan and a mixture of bitter melon 3.6 mL. The phytochemical test results of bitter melon fruit contain carbohydrates, alkaloids, saponins, and terpenoids. The total alkaloid content of the bitter melon fruit is 0.232%, the saponin content of the bitter melon fruit is 0.6375%, and the terpenoid content of the bitter melon fruit is 0.5317%. The administration of bitter melon fruit to diabetic rats can reduce blood sugar levels of rats and the histopathological observations of the pancreas of rats show a repair effect on pancreatic damage after administration of bitter melon herb.

scholarly journals STRATEGY TO MASK THE BITTER TASTE OF MOMORDICA CHARANTIA EXTRACT USING ALGINATE–GELATIN BEADS

2018 ◽  
Vol 10 (1) ◽  
pp. 381
Author(s):  
Sutriyo . ◽  
Raditya Iswandana ◽  
Fauzana Fauzi
Keyword(s):  
Particle Distribution ◽  
Bitter Taste ◽  
Momordica Charantia ◽  
Process Efficiency ◽  
Cross Linking ◽  
Ionic Gelation ◽  
Bitter Melon ◽  
Fruit Extract ◽  
Round Shape ◽  
Melon Fruit

Objective: This study is aimed to develop a strategy to mask the bitter taste of Momordica charantia Linn. extract using alginate–gelatin beads.Methods: Beads of bitter melon fruit extract (M. charantia Linn) were prepared using an ionic gelation method in which cross-linking occurs betweensodium alginate and calcium chloride that serves to mask the bitter taste. Beads were prepared using sodium alginate (1.5% w/v) with variousconcentrations of bitter melon fruit extract (1:2, 1:1, and 1:0.5), gelatin (2% w/v), and CaCl2 3%. The obtained beads were then characterized bothphysically and functionally, and their morphology, process efficiency, particle distribution, swelling index, and water content were recorded.Results: Formula 1 beads, with a 1:2 ratio of extract: alginate, displayed a near-round shape with a diameter of 600–1 200 μm, a swelling index of113.21%, and a moisture content of 15.34%. In addition, these beads were able to significantly cover the bitter taste (p<0.05).Conclusion: Formula 1 beads with a 1:2 ratio of extract: alginate can effectively mask the bitter taste of M. charantia Linn.

Safety Evaluation of Zinc Threoninate Chelate

2010 ◽  
Vol 29 (4) ◽  
pp. 372-379 ◽  
Author(s):  
Xiao-bo Hu ◽  
Yi Gong ◽  
Lei Li ◽  
Shao-ping Nie ◽  
Yuan-xing Wang ◽  
...  
Keyword(s):  
Micronucleus Test ◽  
Lethal Dose ◽  
Clinical Signs ◽  
Organ Weight ◽  
Female Rats ◽  
Male Rats ◽  
Repeat Dose ◽  
Pregnant Rats ◽  
Sperm Abnormality ◽  
Daily Dose

The acute toxicity of zinc threoninate chelate was assessed. The oral lethal dose 50% (LD50) was 2710 mg/kg in female rats and 3160 mg/kg in male rats. Genotoxicity was assessed by Ames test in Salmonella typhimurium strains TA97, TA98, TA100, and TA102, by bone marrow mouse micronucleus test and a sperm abnormality test with mice. Thirty-day repeat dose toxicity study was conducted at oral daily doses of 0, 42, 169, and 675 mg/kg in rats. Teratogenicity was assessed at the same daily dose in pregnant rats by gavage. No significant changes in body weight, food consumption, organ weight, relative organ weight, hematology, blood biochemistry, histopathology, behavior, mortality, sperm abnormality, mutagenicity, and micronucleus formation were observed and no clinical signs or adverse effects were detected. Zinc threoninate chelate had no significant teratogenic effect at a daily dose of 42 mg/kg.

scholarly journals Effect of Tiger Nut Meal on PSA, Relative Organ Weight Sperm Cell and Histological Changes in Androgen-induced Benign Prostate Hyperplasia in Adult Male Wistar Rats

2020 ◽  
pp. 1-10
Author(s):  
D. I. Izunwanne ◽  
J. N. Egwurugwu ◽  
C. L. Emegano
Keyword(s):  
Benign Prostate Hyperplasia ◽  
Relative Weight ◽  
Organ Weight ◽  
Male Rats ◽  
Histological Changes ◽  
Prostate Hyperplasia ◽  
Morphological Studies ◽  
Significant Difference ◽  
Relative Organ Weight ◽  
Benign Prostate

Introduction: It is generally believed that Cyperus esculentus (tiger nut) has some fertility boosting effects. However, scientific validation of some the fertility boosting belief concerning tiger nut is lacking. Objective: The aim of this project was to study the effects of tiger nuts on PSA, Sperm midpiece, relative organ weight and histological changes in BPH induced rats. Method: A total of sixty (60) male rats weighing between 160 – 200 g were used in this study. They were divided into six groups of ten rats per group. Benign prostate hyperplasia was induced in three groups of the rats (as stated in methodology) with 30 mg/kg sub-cutaneous injections of hormones containing dihydrotestosterone (DHT) and estradiol valerate dissolved in olive oil in the ratio of 10:1 (three times in a week, one day interval). Administration of tiger nut meal commenced immediately and lasted for two months. At the end of administration, blood sample was collected from the rat via cardiac puncture for the determination of PSA. Semen sample was also collected for semen morphological studies. Internal organs notably, the prostate and the testes of the rats were also removed for histological examination. Results: The study showed that the induction of BPH brought about some adverse effects. On PSA, the administration of the tiger nut meal ameliorated the BPH by significantly reducing the increased level of the PSA which is a biomarker for prostate hyperplasia (P<0.05). The effect of the tiger nut on sperm morphological toxicities were also examined. Sperm abnormalities like those with bent midpiece was examined. The result showed that the administration of tiger nut meal significantly ameliorated the abnormality and thus, restored the morphology of the sperm cells such that it can enhance fertility. A significant difference was also seen in the relative weight of the prostate. The enlarged prostate in the induced + treated group was later observed through histological studies to have reduced significantly following the administration of the tiger nut. Conclusion: Tiger nut meal ameliorates BPH by reducing the PSA and enlarged prostate. It also ameliorates semen toxicities in the BPH induced + treated rats.

scholarly journals Perbedaan Toksisitas Larutan Buah Pare (Momordica charantia Linnaeus) dan Buah Belimbing Wuluh (Averrhoa bilimbi Linnaeus) Sebagai Biolarvasida Culex Sp

2020 ◽  
Vol 11 ◽  
pp. 60
Author(s):  
Alifatun Khunafa' ◽  
Ngadino Ngadino ◽  
Hadi Suryono ◽  
Aries Prasetyo
Keyword(s):  
Momordica Charantia ◽  
Control Group ◽  
Bitter Melon ◽  
Control Group Design ◽  
Group Design ◽  
Significant Difference ◽  
Tree Fruit ◽  
Post Test ◽  
Culex Sp ◽  
Melon Fruit

One strategy to overcome the case of filariasis is by turning off the vector, namely Culex sp. Mosquitoes, and usually using chemicals as larvicides, in the long run, can cause resistance to larvae and environmental pollution. Bitter melon (Momordica charantia Linnaeus) and cucumber tree fruit (Averrhoa bilimbi Linnaeus) can cause death in larvae because they contain chemical compounds alkaloids, flavonoids, saponins, tannins, and are safe for animals and the environment. This study aims to determine the differences in the toxicity of Bitter melon fruit (Momordica charantia Linnaeus) and cucumber tree fruti (Averrhoa bilimbi Linnaeus) as Culex sp bio-larvacides. The research method was experimental, with the research design used is the post-test only control group design. Samples were Culex sp. third instar mosquito larvae. The study used 4 replications and 7 treatments with 25 larvae of each treatment. The concentration of bitter melon fruit (Momordica charantia Linnaeus) and cucumber tree fruit (Averrhoa bilimbi Linnaeus) were 0% (control); 11%; 22%; 44%. Data analysis was performed analytically using a probit test and a different test (Two Way Anova). The results showed a solution of bitter melon fruit (Momordica charantia Linnaeus) and cucumber tree fruit (Averrhoa bilimbi Linnaeus) had the potential as a bio-larvacide and had a mortality rate at 0% concentration (control), in bitter melon fruit concentrations of 11%; 22%; 44% of 73%, 89%, 100%. Whereas in cucumber tree fruit concentration 11%; 22%; 44; by 95%, 99%, 100%. The different test showed that there was a significant difference between control, bitter melon (Momordica charantia Linnaeus) and cucumber tree fruit (Averrhoa bilimbi Linnaeus) solution to Culex sp larvae mortality (α <0.05). Suggestions that can be given is that bitter melon fruit (Momordica charantia Linnaeus) and cucumber tree fruit (Averrhoa bilimbi Linnaeus) can be used as bio-larvacides and used as alternative larvacides as substitutes for chemical larvacides, and further research using different larvae is needed. Keywords: bio-larvacide; bitter melon fruit solution; cucumber tree solution; Culex sp ABSTRAK Salah satu strategi menanggulangi kasus filariasis adalah dengan cara mematikan vektor yaitu nyamuk Culex sp, dan biasanya menggunakan bahan kimiawi sebagai larvasida dalam jangka panjang dapat menyebabkan resistensi pada larva dan pencemaran lingkungan. Buah pare (Momordica charantia linnaeus) dan buah belimbing wuluh (Averrhoa bilimbi linneus) dapat menyebabkan kematian pada larva karena mengandung senyawa kimia alkaloid, flavonoid, saponin, tanin, serta bersifat aman terhadap hewan dan bagi lingkungan. Penelitian ini bertujuan untuk mengetahui perbedaan toksisitas larutan buah pare (Momordica charantina linnaeus) dan buah belimbing wuluh (Averrhoa bilimbi linnaeus) sebagai biolarvasida Culex sp. Metode penelitian adalah eksperimental, dengan rancangan penelitian yang digunakan adalah post test only control group design. Sampel berupa larva nyamuk Culex sp instar III. Penelitian menggunakan 4 replikasi dan 7 perlakuan dengan jumlah larva uji masing-masing perlakuan sebanyak 25 ekor. Konsentrasi buah pare (Momordica charantia linnaeus) dan buah belimbing wuluh (Averrhoa bilimbi linneus) yaitu 0% (kontrol); 11% ; 22% ; 44%. Analisis data dilakukan secara analitik menggunakan uji probit dan uji beda (Two Way Anova).Hasil penelitian menunjukkan larutan buah pare (Momordica charantia linnaeus) dan buah belimbing wuluh (Averrhoa bilimbi linnaeus) berpotensi sebagai biolarvasida dan memiliki angka kematian pada konsentrasi 0% (kontrol), pada buah pare konsentrasi 11% ; 22% ; 44% sebesar 73%, 89%, 100%. Sedangkan pada buah belimbing wuluh konsentrasi 11% ; 22% ; 44; sebesar 95%, 99%, 100%. Uji beda menunjukkan terdapat perbedaan yang signifikan antara kontrol, larutan buah pare (Momordica charantia linnaeus) dan larutan buah belimbing wuluh (Averrhoa bilimbi linnaeus) terhadap kematian larva nyamuk Culex sp (α < 0,05). Saran yang dapat diberikan adalah agar buah pare (Momordica charantia linnaeus) dan buah belimbing wuluh (Averrhoa bilimbi linnaeus) dapat dimanfaatkan sebagai biolarvasida dan dijadikan sebagai larvasida alternatif sebagai pengganti larvasida kimia, serta diperlukan penelitian lebih lanjut dengan menggunakan larva yang berbeda. Kata kunci: biolarvasida; larutan buah pare; larutan buah belimbing wuluh; Culex sp.

scholarly journals Studies of dietary phytoestrogen from momordica charantia Linn. Seeds

2020 ◽  
pp. 26-33
Author(s):  
Sharanabasappa Patil
Keyword(s):  
Spectral Analysis ◽  
Ethanol Extract ◽  
Momordica Charantia ◽  
Human Consumption ◽  
Preparative Hplc ◽  
Bitter Melon ◽  
Plant Metabolites ◽  
Secondary Plant Metabolites ◽  
Amorphous Compound ◽  
Melon Seeds

Phytoestrogens are secondary plant metabolites produced by plants that are similar in structure of estrogen. Human consumption of these compounds has been associated with many health benefits. In this study, we investigated the potential phytoestrogen content of dietary Momordica charantia Linn. (Bitter melon) seeds were collected from the Hyderabad-Karnataka regions of India. A phenolic dietary phytoestrogen has been isolated from the crude ethanol extract of Bitter melon seeds. After preparative HPLC whitish amorphous compound was yielded. The HPLC purified compound is subjected to spectral analysis using IR, NMR and MS. The spectral data revealed that the phenolphthalein a phytoestrogenic molecule is present in the ethanol extract of dietary Bitter melon seeds.

scholarly journals Bitter Melon (Momordica charantia L) Fruit Decreased Blood Glucose Level and Improved Lipid Profile of Streptozotocin Induced Hyperglycemia Rats

2018 ◽  
Vol 6 (2) ◽  
pp. 359-370 ◽  
Author(s):  
Ummi Rohajatien ◽  
Harijono Harijono ◽  
Teti Estiasih ◽  
Endang Sriwahyuni
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Lipid Profile ◽  
Dietary Fiber ◽  
Glucose Level ◽  
Cholesterol Absorption ◽  
Momordica Charantia ◽  
Bitter Melon ◽  
Insoluble Dietary Fiber ◽  
Melon Fruit

Bitter melon (Momordica charantia, L) is a fruit that traditionally believe has benefits on health. The objective of this study is to identify bitter melon bioactive and nutritional compounds, and their effect on blood glucose level and lipid profile of streptozotocin induced hyperglycemia rats. Rats were divided into three group, those were normal group; hyperglycemia group without bitter melon fruit feeding; and hyperglycemia group with bitter melon fruit administration. Hyperglycemia condition was achieved by STZ induction. The experiment was conducted for 4 weeks. The results showed that fresh bitter melon fruit contains β-sitosterol 348.16+1.66 ppm, stigmasterol 183.08+0.8 ppm, campesterol 130.79+0.4 ppm, diosgenin 16.42+0.06 ppm, soluble dietary fiber 2.99+0.07%, insoluble dietary fiber 0.55+0.01%, and pectin 1.41+0.05%. At week 4 of experiment, bitter melon fruit fed hyperglycemia group showed a decrease of 56% blood glucose level compared to blood glucose level at week 0. Body weight of this group also increased. The improvement of lipid profile of bitter melon fed group was indicated by decreasing blood total cholesterol of 49%, triglyceride of 35%, LDL cholesterol of 42%, and increasing HDL of 133% compared to initial level at week 0. Bitter melon also increased fecal cholesterol secretion and effectively inhibited cholesterol absorption in hyperglycemia rats. Bitter melon fruit is suggested for hyperglycemia management due to its ability to reduce glucose and improve lipid profile simultaneously.

scholarly journals Terapi Infusa Pekat Buah Pare (Momordica charantia L.) terhadap Kadar Glukosa Darah dan SOD pada Ginjal Tikus DM Tipe 1

ALCHEMY ◽  
2019 ◽  
Vol 6 (2) ◽  
pp. 43
Author(s):  
Nuraeni Uswatun Hasanah ◽  
Hafidatul Hasanah ◽  
Hummatul Barroroh
Keyword(s):  
Diabetes Mellitus ◽  
Blood Sugar ◽  
Blood Sugar Level ◽  
Rat Kidney ◽  
Momordica Charantia ◽  
Bitter Melon ◽  
Sod Activity ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Melon Fruit

<p>Bitter melon (<em>Momordica charantia</em> L.) is one of the <em>Cucurbitaceae</em> families which is useful as traditional antidiabetic medicine. Diabetes mellitus (DM) is an autoimmune disease indicated by increasing blood sugar levels. The aim of this study to determine the effect of concentrated infusions therapy of bitter melon fruit on blood glucose levels and SOD activity in three diabetic levels (low, medium, acute) in DM Type 1 of rat kidney. The doses of therapy used were 0.15, 0.30, 0.45, 0.60, 0.80 and 1 mL/200 g body weight (BW). Blood sugar level was measured using enzymatic (glucose oxidase) method and SOD activity using NBT (Nitroblue tetrazolium) test. The results showed that the concentrated infusions therapy of bitter melon fruit was able to decrease blood glucose levels and increase the SOD activity of DM 1 in the rat kidney. The average ability of the therapy to reduce DM in low, medium, and acute level was 87.21, 84.5 and 55,56%, respectively. In 0.3 mL/200 g Bw dose, concentrated infusions therapy of bitter melon fruit reduced blood sugar level to all diabetes levels. The average value of concentrated infusions therapy of bitter melon fruit in increasing SOD activity was 144.76%.</p><p> </p><p>Keywords:  Diabetes mellitus, infuse, SOD (superoxide dismutase), bitter melon fruit <em>(Momordica charantia L.)</em></p><p> </p><p>Buah pare (<em>Momordica charantia </em>L.) merupakan salah satu keluarga <em>Cucurbitaceae</em> yang bermanfaat sebagai obat tradisional, salah satunya untuk antidiabetes. Diabetes mellitus (DM) merupakan salah satu penyakit autoimun yang ditandai dengan meningkatnya kadar gula darah (KGD). Penelitian ini bertujuan untuk mengetahui pengaruh terapi infusa pekat buah pare terhadap kadar glukosa darah dan SOD pada tiga tingkat diabetes yaitu ringan, sedang dan akut pada ginjal tikus DM tipe 1. Dosis yang digunakan yakni 0,15; 0,30; 0,45; 0,60; 0,80; dan 1 mL/200 g BB. KGD diiukur dengan metode enzimatik (glukosa oksidase) dan aktivitas SOD diukur dengan uji NBT (<em>Nitroblue tetrazolium</em>). Hasil penelitian menunjukkan terapi infusa pekat buah pare berpengaruh terhadap penurunan kadar glukosa darah dan peningkatan kadar SOD pada ginjal tikus DM 1. Kemampuan rata-rata terapi dalam menurunkan DM tingkat ringan, sedang, dan akut masing-masing sebesar 87,21; 84,5 dan 55,56%. Pada dosis 0,3 mL/200 g BB, terapi infusa pekat buah pare mampu menurunkan KGD terhadap seluruh tingkat DM. Kemampuan rata-rata infusa pekat buah pare dalam meningkatkan kadar SOD sebesar 144,76%.</p><p> </p>Kata Kunci: Diabetes mellitus, infusa, SOD (superoksida dismutase), buah pare (<em>Momordica charantia</em> L.)

scholarly journals Efecto anticonceptivo y postcoital del extracto etanólico de lashojas del Desmodium molliculum (HBK).DC “Manayupa” en ratas hembras Holtzmann

2019 ◽  
pp. 33-41
Keyword(s):  
Statistical Tests ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Alcoholic Solution ◽  
Female Rats ◽  
Male Rats ◽  
Experimental Conditions ◽  
Contraceptive Effect ◽  
Postcoital Contraception ◽  
Group 2

Efecto anticonceptivo y postcoital del extracto etanólico de lashojas del Desmodium molliculum (HBK).DC “Manayupa” en ratas hembras Holtzmann Postcoital contraceptive effect of ethanolic extract of the leaves of Desmodium molliculum (HBK).DC "Manayupa" in Holtzmann female rats Fidel Ernesto Acaro Chuquicaña Facultad de Medicina, Universidad Nacional Mayor de San Marcos DOI: https://doi.org/10.33017/RevECIPeru2012.0018/ Resumen Nuestro objetivo fue determinar el efecto anticonceptivo y postcoital del extracto etanólico de las hojas de Desmodium molliculum (HBK).DC en ratas hembras adultas Holtzmann. Las hojas fueron recolectadas en el valle de Baños del Inca, departamento de Cajamarca, la cual es utilizada como diurética, depurativa de la sangre, antihemorrágica, antiinflamatoria de las vías urinarias, hígado y riñones. Se utilizaron 80 ratas hembras fueron seleccionadas aleatoriamente de acuerdo a los criterios de inclusión, y divididas en 2 grandes grupos, cada uno conformada por 5 subgrupos de 8 ratas hembras y ratas machos para el emparejamiento (1 macho: 2 hembras). Grupo 1: 40 ratas para la evaluación del efecto anticonceptivo del extracto de Desmodium molliculum a una solución 100mg/ml vía oral en dosis 200mg/kg, 600mg/kg y 1000mg/kg. Se utilizaron dos grupos como controles suero fisiológico y Medroxiprogesterona en dosis 15mg/kg; Grupo 2: 40 ratas para la evaluación del efecto postcoital del extracto Desmodium molliculum a una solución de 100mg/ml vía oral a dosis 200mg/kg, 600mg/kg y 1000mg/kg. Se utilizaron dos grupos control suero fisiológico y Levonorgestrel a dosis de 50ug/kg. El efecto anticonceptivo se evaluó mediante la cuantificación de los indicadores gravidez, número de implantaciones y número de fetos; el efecto post-coital se evaluó los indicadores gravidez, número de implantaciones, número de fetos vivos y número de fetos muertos. Del escreening fitoquímico se observa que las muestras en estudio, presentan una considerable cantidad de metabolitos secundarios, destacándose cantidades apreciables de flavonoides, compuestos fenólicos, esteroides, alcaloides y taninos estuvieron en mayor cantidad en el extracto etanólico. De las evaluaciones las ratas que recibieron la solución alcohólica a dosis de 200mg/kg.600mg/kg,1000mg/kg disminuyeron la gravidez, el número fetos e implantaciones en la actividad anticonceptiva y poscoital. Se realizaron análisis descriptivos y pruebas estadísticas de significancia ANOVA y Tukey (p<0.05).Concluyendo que en condiciones experimentales los resultados encontrados demuestran que el extracto etanólico de Desmodium molliculum tiene efecto anticonceptivo y postcoital en ratas hembras Holtzmann. Descriptores: Anticonceptivo, Postcoital, Desmodium molliculum, Plantas Anticonceptiva. Abstract Our objective was to determine the effect of postcoital contraception and ethanol extract of the leaves of Desmodium molliculum (HBK). DC Holtzmann in adult female rats. The leaves were collected in the valley of Baños del Inca, Cajamarca department, which is used as a diuretic, purifying the blood, prevent bleeding, inflammatory urinary tract, liver and kidneys. We used 80 female rats were randomly selected according to the inclusion criteria, and divided into 2 groups, each consisting of 5 subgroups of 8 male rats and female rats for pairing (1 male: 2 females). Group 1: 40 rats to evaluate the contraceptive effect of the extract of Desmodium molliculum a 100mg/ml solution orally at 200mg/kg, 600mg/kg and 1000mg/kg. Two groups as saline controls and 15mg/kg dose medroxyprogesterone. Group 2: 40 rats to evaluate the effect of post-coital Desmodium molliculum extract a 100mg/ml solution orally at 200mg/kg dose, 600 mg / kg and 1000mg/kg. Two control groups were used and Levonorgestrel saline at doses of 50ug/kg. The contraceptive effect was assessed by quantifying indicators pregnancy, number of locations and number of fetuses; the effect was evaluated post-coital pregnancy indicators, number of implantations, number of live fetuses and dead fetuses number. Phytochemical screening shows that the samples under study show a considerable amount of secondary metabolites, highlighting significant amounts of flavonoids, phenolic compounds, steroids, alkaloids and tannins were in greater amounts in the ethanol extract. Evaluation of rats receiving a dose of alcoholic solution 200mg/kg.600mg/kg, 1000mg/kg decreased pregnancy, fetuses and the number deployments and postcoital contraceptive activity. Descriptive analyzes were performed and statistical tests of significance ANOVA and Tukey (p <0.05). Concluding that under experimental conditions the results show that the ethanol extract of Desmodium molliculum effect and postcoital contraception in female rats Holtzmann. Keywords: Anticonceptivo, Postcoital, Desmodium molliculum, Plantas Anticonceptiva.

Sign in / Sign up

Export Citation Format

Share Document