Lipoxygenase activity and antioxidant capacity in pepper (Capsicum annum) exposed to high concentration of copper sulphate

Citrus fruit is preferred in the choice of consumers. Kumquat (F. margarita) is an unconventional citrus of increasing consumer interest because of its exotic flavor, and its functional potential that offers health benefits to consumers. It is a fruit traditionally consumed by whole fruit (peel and pulp), giving this fruit a distinctive flavor. For this reason, this study analyzed the physical, chemical, and nutritional characteristics of kumquat (peel and pulp). The physicochemical analysis was performed according to the Adolfo Lutz Institute. Analysis of moisture, ashes, macronutrients, and total dietary fiber was carried out according to AOAC. Minerals were analyzed by inductively coupled plasma optical emission spectrometry. Vitamins C and E, carotenoids and flavonoids were analyzed by HPLC. Phenolic compounds (Folin-Ciocalteu) and antioxidant capacity (DPPH) were determined by spectrophotometry. The kumquat had low pH, soluble solids content and low caloric value. It was a source of dietary fiber, minerals (K, Ca, P, Mg) and carotenoids; the most expressive was α-carotene (661.81 μg 100 g−1). The ascorbic acid concentration was 2326.24 μg 100 g−1. α-tocopherol (569.00 μg 100 g−1) was the most expressive component of vitamin E. There was a presence of apigenin and eriodictyol. The fruit (peel and pulp) has a high concentration of total phenolic compounds (98.55 ± 1.93 mg GAE 100 g−1) and good antioxidant capacity (62%) was found. Kumquat is a good source of fiber and vitamin A, and due to its antioxidant capacity and the presence of other essential and beneficial nutrients for a diet, consumption of kumquat can be suggested to complement the diet. This fruit is a viable food alternative, and its consumption should be encouraged, contributing a source of income, sovereignty, and food security.

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Growth Performance, Antioxidant Capacity, Lipid-Related Transcript Expression and the Economics of Broiler Chickens Fed Different Levels of Rutin

Animals ◽

10.3390/ani9010007 ◽

2018 ◽

Vol 9 (1) ◽

pp. 7 ◽

Cited By ~ 3

Author(s):

Fardos Hassan ◽

Elshimaa Roushdy ◽

Asmaa Kishawy ◽

Asmaa Zaglool ◽

Hammed Tukur ◽

...

Keyword(s):

Antioxidant Capacity ◽

Growth Performance ◽

Broiler Chickens ◽

Fatty Acid Synthase ◽

Body Weight Gain ◽

Density Lipoprotein ◽

Basal Diet ◽

Peroxisome Proliferator ◽

High Concentration ◽

Peroxisome Proliferator Activated Receptor

The effects of rutin on growth performance, hematological and biochemical profiles, antioxidant capacity, economics and the relative expression of selected antioxidants and lipid-related genes were studied in broiler chickens over 42 days. A total of 200 one-day-old female Ross-308 broiler chickens were distributed into four groups, with five replicates of 10 individuals per replicate. They were fed with 0 (control), 0.25, 0.5 or 1 g rutin/kg supplementation in their basal diet. Dietary rutin supplementation, especially the 1 g/kg diet, increased body weight gain, the protein efficiency ratio (p < 0.001) and both white blood cell and lymphocyte counts (p < 0.001). However, it had no effect on total protein, albumin, globulin, or alanine transaminase. A high concentration of rutin (0.5 and 1 g/kg) also significantly reduced serum total cholesterol, triacylglycerol and low-density lipoprotein cholesterol concentrations (p < 0.001), as well as malondialdehyde concentrations (p = 0.001). A high concentration diet also increased the activity of superoxide dismutase, catalase and glutathione peroxidase. Of the lipid-related genes examined, acetyl CoA carboxylase and fatty acid synthase were significantly down-regulated in the livers of rutin-fed individuals, whereas carnitine palmitoyl transferase 1 and peroxisome proliferator-activated receptor alpha were significantly up-regulated. Therefore, rutin supplementation at 1 g/kg has the potential to improve the productive performance and health status of broiler chickens.

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LC-ESI-QTOF/MS Characterization of Phenolic Compounds from Medicinal Plants (Hops and Juniper Berries) and Their Antioxidant Activity

Foods ◽

10.3390/foods9010007 ◽

2019 ◽

Vol 9 (1) ◽

pp. 7 ◽

Cited By ~ 9

Author(s):

Jiafei Tang ◽

Frank R. Dunshea ◽

Hafiz A. R. Suleria

Keyword(s):

Antioxidant Activity ◽

Phenolic Compounds ◽

Medicinal Plants ◽

Antioxidant Capacity ◽

Phenolic Acids ◽

Phenolic Content ◽

Total Phenolic ◽

High Concentration ◽

Antioxidant Power ◽

Pharmaceutical Industries

Hops (Humulus lupulus L.) and juniper berries (Juniperus communis L.) are two important medicinal plants widely used in the food, beverage, and pharmaceutical industries due to their strong antioxidant capacity, which is attributed to the presence of polyphenols. The present study is conducted to comprehensively characterize polyphenols from hops and juniper berries using liquid chromatography coupled with electrospray-ionization quadrupole time-of-flight mass spectrometry (LC-ESI-QTOF/MS) to assess their antioxidant capacity. For polyphenol estimation, total phenolic content, flavonoids and tannins were measured, while for antioxidant capacity, three different antioxidant assays including the 2,2-diphenyl-1-picrylhydrazyl (DPPH) antioxidant assay, the 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical cation decolorization assay and the ferric reducing-antioxidant power (FRAP) assay were used. Hops presented the higher phenolic content (23.11 ± 0.03 mg/g dw) which corresponded to its strong antioxidant activity as compared to the juniper berries. Using LC-ESI-QTOF/MS, a total of 148 phenolic compounds were tentatively identified in juniper and hops, among which phenolic acids (including hydroxybenzoic acids, hydroxycinnamic acids and hydroxyphenylpropanoic acids) and flavonoids (mainly anthocyanins, flavones, flavonols, and isoflavonoids) were the main polyphenols, which may contribute to their antioxidant capacity. Furthermore, the HPLC quantitative analysis showed that both samples had a high concentration of phenolic acids and flavonoids. In the HPLC quantification, the predominant phenolic acids in hops and juniper berries were chlorogenic acid (16.48 ± 0.03 mg/g dw) and protocatechuic acid (11.46 ± 0.03 mg/g dw), respectively. The obtained results highlight the importance of hops and juniper berries as a rich source of functional ingredients in different food, beverage, and pharmaceutical industries.

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Physical-chemical properties of exotic and native Brazilian fruits

Acta Agronómica ◽

10.15446/acag.v68n3.55934 ◽

2019 ◽

Vol 68 (3) ◽

pp. 175-181 ◽

Cited By ~ 1

Author(s):

Veridiana Zocoler de Mendonça ◽

Rogério Lopes Vieites

Keyword(s):

Phenolic Compounds ◽

Antioxidant Capacity ◽

Chemical Properties ◽

Titratable Acidity ◽

Soluble Solids ◽

High Concentration ◽

Bioactive Substances ◽

Fruit Species ◽

Physical Chemical ◽

Spondias Purpurea

Many fruit species are still not well-studied, despite being rich in bioactive substances that have functional properties. The objective of this article was to evaluate the antioxidant potential and characterize the physical-chemical characteristics of unconventional brazilian fruits (cabeludinha - Myrciaria glazioviana, sapoti - Manilkara zapota, pitomba - Talisia esculenta, yellow gumixama - Eugenia brasiliensis var. Leucocarpus and seriguela - Spondias purpurea). Total soluble solids, pH, titratable acidity, sugars, pigments, phenolic compounds and antioxidant capacity were measured. Mature fruits were used in the analyses. Pitomba had high levels of soluble solids, 24.6 °Brix, while sapoti had 0.05 g malic acid 100 g-1 pulp. Yellow grumixama and seriguela had the highest concentrations of anthocyanins and carotenoids. Cabeludinha had a high concentration of phenolic compounds, 451.60 mg gallic acid 100 g-1 pulp. With the exception of sapoti, all fruits had a high antioxidant capacity (> 95%).

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Application of high-dose propofol during ischemia improves postischemic function of rat hearts: effects on tissue antioxidant capacity

Canadian Journal of Physiology and Pharmacology ◽

10.1139/y04-097 ◽

2004 ◽

Vol 82 (10) ◽

pp. 919-926 ◽

Cited By ~ 18

Author(s):

Zhengyuan Xia ◽

David V Godin ◽

David M Ansley

Keyword(s):

Lipid Peroxidation ◽

Antioxidant Capacity ◽

Functional Recovery ◽

Isolated Heart ◽

Cardiac Mechanics ◽

Heart Tissue ◽

Global Ischemia ◽

High Dose ◽

High Concentration ◽

Heart Preparation

Previous studies have shown that reactive oxygen species mediated lipid peroxidation in patients undergoing cardiac surgery occurs primarily during cardiopulmonary bypass. We examined whether application of a high concentration of propofol during ischemia could effectively enhance postischemic myocardial functional recovery in the setting of global ischemia and reperfusion in an isolated heart preparation. Hearts were subjected to 40 min of global ischemia followed by 90 min of reperfusion. During ischemia, propofol (12 µg/mL in saline) was perfused through the aorta at 60 µL/min. We found that application of high-concentration propofol during ischemia combined with low-concentration propofol (1.2 µg/mL) administered before ischemia and during reperfusion significantly improved postischemic myocardial functional recovery without depressing cardiac mechanics before ischemia, as is seen when high-concentration propofol was applied prior to ischemia and during reperfusion. The functional enhancement is associated with increased heart tissue antioxidant capacity and reduced lipid peroxidation. We conclude that high-concentration propofol application during ischemia could be a potential therapeutic and anesthetic strategy for patients with preexisting myocardial dysfunction.Key words: propofol, ischemia, heart, rat, oxidative stress.

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Comprehensive multivariate correlations between climatic effect, metabolite-profile, antioxidant capacity and antibacterial activity of Brazilian red propolis metabolites during seasonal study

Scientific Reports ◽

10.1038/s41598-019-54591-3 ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 3

Author(s):

Ticiano Gomes do Nascimento ◽

Rodolfo Elleson dos Santos Arruda ◽

Erika Tayse da Cruz Almeida ◽

José Marcos dos Santos Oliveira ◽

Irinaldo Diniz Basílio-Júnior ◽

...

Keyword(s):

Antibacterial Activity ◽

Antioxidant Capacity ◽

Metabolite Profile ◽

Climatic Effect ◽

High Concentration ◽

Climate Effect ◽

Drought Period ◽

Rainy Period ◽

Vip Analysis ◽

Multivariate Correlations

AbstractThe standardization of apiceutical products like as propolis extracts has been widely debated worldwide and variations in the propolis chemical composition are still very relevant topics for use-standardized of different propolis-type as medication by much of the world’s population. The present manuscript discuss important issues related to the climate effect and variations in propolis metabolite-profiling changes, antioxidant capacity and variations of the antibacterial activity of the Brazilian red propolis metabolites using comprehensive multivariate correlations. It was observed the increasing of guttiferones concentrations during the intense drought period and drastic decreasing in rainy period. The climate variation induced the high concentration of flavonoids in rainy period with pronounced dropped in some rainy months. The Pearson´s analysis demonstrated correlation between IC50 from DPPH and guttiferones and flavonoids concentrations. The PCA-X and Hotelling T2 test showed outliers during the months with lowest concentrations of formononetin and isoliquiritigenin was observed in antibacterial tests. The PLS-DA, OPLS-DA and VIP analysis demonstrate guttiferone E, guttiferone B, liquiritigenin, naringenin are considered important substances responsible by anti-staphylococcal activity in red propolis composition during the rainy season and drought period, but a synergistic effect with other flavonoids and isoflavonoids are not ruled out.

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Characterization and Evaluation of Ternary Complexes of Ascorbic Acid with γ-Cyclodextrin and Poly(vinyl Alcohol)

International Journal of Molecular Sciences ◽

10.3390/ijms21124399 ◽

2020 ◽

Vol 21 (12) ◽

pp. 4399

Author(s):

Phennapha Saokham ◽

Kanokporn Burapapadh ◽

Pitsiree Praphanwittaya ◽

Thorsteinn Loftsson

Keyword(s):

Ascorbic Acid ◽

Antioxidant Capacity ◽

Vinyl Alcohol ◽

Ternary Complex ◽

Ternary Complexes ◽

Hydroxyl Group ◽

Poly Vinyl Alcohol ◽

Hydrodynamic Diameter ◽

High Concentration ◽

Drug Stabilization

Ascorbic acid (AA) is a general antioxidant used in aqueous pharmaceutical formulations. However, in aqueous solutions, AA is unstable and easily oxidized when exposed to air, light and/or heat. Cyclodextrins are well known for their ability to form inclusion complexes with various compounds to improve their solubility and stability. Previous studies demonstrate that cyclodextrins preserve the antioxidant capacity of AA but data for γ-cyclodextrin (γCD) have not been reported. Poly(vinyl alcohol) (PVA) is a hydrophilic polymer widely used as a drug matrix in various pharmaceutical fields, but its application for drug stabilization is limited. This study aimed to investigate the protective ability of γCD on AA through the formation of ternary complexes with PVA. Binary (i.e., AA/γCD, AA/PVA and γCD/PVA) and ternary (i.e., AA/γCD/PVA) complexes were first confirmed. It was reported that those complexes were formed through interactions between the heterocyclic ring of AA, hydroxyl group of PVA and hydrophobic cavity of γCD. The hydrodynamic diameter of complexes was then studied. It was found that the diameter of γCD/PVA complexes increased with respect to the concentration of γCD. Higher γCD concentrations also resulted in increasing hydrodynamic diameters of the ternary complex. The presence of AA in ternary complexes interfered with the aggregation tendency of γCD/PVA binary complexes. Furthermore, the antioxidant capacity of AA in binary and ternary complexes was investigated. It was found that the presence of γCD preserved the antioxidant activity of AA, whereas PVA showed a contrasting effect. The influence of γCD and PVA concentration on antioxidant capacity was then studied through central composite design (CCD). Even though the concentration of γCD significantly affected the inhibition efficiency of the ternary complex, the insignificant influence of PVA could not be ignored. A promising protective ternary complex should consist of an optimized concentration of PVA and a high concentration of γCD.

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Root inoculation of green bell pepper ( Capsicum annum ) with Bacillus amyloliquefaciens BBC047: effect on biochemical composition and antioxidant capacity

Journal of the Science of Food and Agriculture ◽

10.1002/jsfa.9758 ◽

2019 ◽

Vol 99 (11) ◽

pp. 5131-5139 ◽

Cited By ~ 2

Author(s):

Jonathan Cisternas‐Jamet ◽

Ricardo Salvatierra‐Martínez ◽

Antonio Vega‐Gálvez ◽

Elsa Uribe ◽

María G Goñi ◽

...

Keyword(s):

Antioxidant Capacity ◽

Biochemical Composition ◽

Bacillus Amyloliquefaciens ◽

Bell Pepper ◽

Capsicum Annum

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Nutritional characterization of some natural plants used in poultry nutrition

Archiva Zootechnica ◽

10.2478/azibna-2020-0013 ◽

2020 ◽

Vol 23 (2) ◽

pp. 58-72

Author(s):

Turcu Raluca Paula ◽

Olteanu Margareta ◽

Untea Arabela Elena ◽

Saracila Mihaela ◽

Varzaru Iulia ◽

...

Keyword(s):

Antioxidant Capacity ◽

Animal Feed ◽

Proximate Analysis ◽

Point Of View ◽

Antioxidant Compounds ◽

High Concentration ◽

Total Polyphenols ◽

Animal Products ◽

Natural Plants ◽

Poultry Nutrition

Abstract Current nutritional strategies of livestock industry are focused on evaluating the effects of terrestrial sources rich in natural bioactive compounds that can be used in farm animal feed and the subsequent implications on the quality of resulting animal products. In this context, the present study aimed to characterize from a nutritional point of view some natural plants used as phyto-additives in poultry nutrition: oregano, mint, basil, sage, fenugreek, thyme, turmeric, cumin and rosemary. The results of this study on plants nutritional evaluation showed a varied proximate analysis. Of all the plants, cumin, fenugreek and basil were the richest source of crude protein. Thyme had a large ether extractives content, followed by rosemary, sage and cumin. The obtained results revealed that oregano has the strongest antioxidant capacity (849.77 mmols equiv. asc. acid; 863.57 mmols equiv. vit. E), the highest total polyphenols concentration (86.77 mg GAE/g) and lutein and zeaxanthin (304.23 μg/g) of the analysed plants. Nevertheless, all plants had high concentration of total polyphenols, except cumin, a large amount of xanthophylls and vitamin E. After oregano, sage and thyme have been noted for their antioxidant capacity and major antioxidant compounds. Basil and sage revealed the highest amount of essential trace elements.

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Physicochemical quality, antioxidant capacity and nutritional value of edible flowers of some wild dahlia species

Folia Horticulturae ◽

10.2478/fhort-2019-0026 ◽

2019 ◽

Vol 31 (2) ◽

pp. 331-342 ◽

Cited By ~ 2

Author(s):

Esteban Arturo Rivera Espejel ◽

Oscar Cruz Alvarez ◽

José Merced Mejía Muñoz ◽

María del Rosario García Mateos ◽

María Teresa Beryl Colinas León ◽

...

Keyword(s):

Antioxidant Capacity ◽

Nutritional Value ◽

Eating Habits ◽

Titratable Acidity ◽

Nutritional Composition ◽

Soluble Solids ◽

Fresh Weight ◽

High Concentration ◽

Physicochemical Quality ◽

Centre Of Origin

AbstractMexico is the centre of origin and domestication of the dahlia, which has the widest distribution worldwide as an ornamental plant; however, due to changes in eating habits in some sectors of the population (vegetarians and vegans), it has also been incorporated as an ingredient in the gastronomic field. The aim of this research was to determine the attributes of physicochemical quality, antioxidant capacity and nutritional value of ligulate flowers of some wild dahlia species. A completely randomized experimental design with five repetitions was used. Fresh weight, total titratable acidity (TTA), total soluble solids (TSS), ascorbic acid (AA), colour, total phenols (TP), total flavonoids (TFl), total anthocyanins (TAn), antioxidant capacity (AC) and proximate composition were evaluated. The highest fresh weight was observed in D. × hortorum, with similar values among its different flower colours. Likewise, D. merckii and D. coccinea stood out for their high TTA value; however, D. coccinea also had the highest AA content (0.77-0.9 mg g−1 FW), TP (12.87-19.92 mg GAE g−1 FW), TFl (6.78-11.32 mg QE g−1 DW) and AC (47.28-60.43 mg AAEVC g−1 FW). On the other hand, D. campanulata was notable for its high levels of moisture, fat and raw fibre; similarly, D. coccinea stood out for its high amounts of dry matter and total carbohydrates. The ligulate flowers of wild dahlias have a high concentration of bioactive compounds and a nutritional composition with beneficial contributions to health care, so they could be used for consumption as fresh products.

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